CN105699145A - Method for preparing transmission electron microscope sample by embedding drosophila melanogaster in agar - Google Patents
Method for preparing transmission electron microscope sample by embedding drosophila melanogaster in agar Download PDFInfo
- Publication number
- CN105699145A CN105699145A CN201610103594.8A CN201610103594A CN105699145A CN 105699145 A CN105699145 A CN 105699145A CN 201610103594 A CN201610103594 A CN 201610103594A CN 105699145 A CN105699145 A CN 105699145A
- Authority
- CN
- China
- Prior art keywords
- embedding
- fruit bat
- agar
- drosophila melanogaster
- conditions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2806—Means for preparing replicas of specimens, e.g. for microscopal analysis
Abstract
The invention provides a method for preparing a transmission electron microscope sample by embedding a drosophila melanogaster in agar. The method comprises the steps that the drosophila melanogaster with no head is embedded in an agar powder solution with the mass fraction of 2%, a solidified agar block is pruned by a two-face blade to be a cuboid according to the body of the drosophila melanogaster, the size of the cuboid is three times that of the drosophila melanogaster, and the back of the drosophila melanogaster is attached to the face, with the smallest area, of the cuboid and is parallel to the face; the drosophila melanogaster is fixed with osmic acid again; gradient ethanol-acetone dehydration and embedding medium soakage are performed; during embedding, the face to which the back of the drosophila melanogaster is tightly attached and parallel abuts against the headmost end of an embedding plate module; 2-micrometer semithin section is cut after polymerization, the section is dyed by toluidine blue with the mass fraction of 1%, a longitudinal section of the muscle of the back of the drosophila melanogaster is obtained, and positioning of a target position is achieved. The method for positioning the longitudinal section of the muscle of the back of the drosophila melanogaster by embedding the drosophila melanogaster with no head in the agar is simpler and more accurate compared with a conventional electron microscope processing method, and can be widely applied to any sample with the specific position needing to be positioned in the technical field of electron microscopes.
Description
Technical field
The invention belongs to cell biology, specifically the method for preparing transmission electron microscopy of agar embedding fruit bat。
Background technology
At present for having horny layer, the transmission electron microscope sample that the glutaraldehydes such as heavy wall not easily penetrate, generally take to use 4% paraformaldehyde (or 2% paraformaldehyde)-2.5% glutaraldehyde mixed stationary liquid to replace single glutaraldehyde fixative, or in glutaraldehyde solution, add the penetration power of TritonX-100 raising fixative, so during preparation fruit bat sample, being directly lose fruit bat in fixative to process。Embedding medium embedding uses the embedding pipe of capsule, centrifuge tube or centrifugal tubular type often, fruit bat back is attached at the bottom of capsule bottom or centrifuge tube pipe during embedding, but finding after being polymerized that most fruit bat has been waftd on the centre of resin mass or surface all, the back, target location that namely we need varies by。Most embedding mediums need the high temperature of 60 DEG C, 70 DEG C when polymerization, and at these elevated temperatures, the air in pipe is constantly discharged outward, owing to fruit bat body is light, can move under the drive of air。For this kind of situation, that commonly uses at present has two kinds of solutions, and one is the angle of diamant on rotary microtome, makes the edge of a knife parallel with fruit bat back, is so not easy to have a shave and ultrathin section, and the diamond cutter used when returning ultrathin section buries potential safety hazard；Two is cut off from resin mass by sample, readjusts position and carries out secondary or three embeddings, and this to use small-sized rustless steel saw blade, relatively elaborate。Only processing one or two such sample more relatively easily, but each experimental group has several repetition, two kinds of processing methods obviously all waste time and energy, efficiency is low。
At present the orientation positions of fruit bat muscle of back is also still lacked to method simple, Fast Practical。
Summary of the invention
It is an object of the invention to provide the method for preparing transmission electron microscopy of a kind of agar embedding fruit bat。
The technical scheme solving above-mentioned technical problem of the present invention is as follows:
The method for preparing transmission electron microscopy of agar embedding fruit bat, is prepare transmission electron microscope sample with agar solution after being embedded in advance by fruit bat to realize the method quickly positioning fruit bat muscle of back, and operating procedure is as follows:
1. fruit bat is taken out after ether is smoked and swooned, and with double-edged razor blade, head quickly cuts (artificial tear fracture head portion), is lost by fruit bat immediately in the glutaraldehyde fixative that volume fraction is 3%, and evacuation makes it sink at the bottom of pipe, fixes 2 hours under 4 DEG C of conditions。
2. preparing the agar powder solution that mass fraction is 2% in advance, put into 4 DEG C of Refrigerator stores, used time microwave-oven-heating dissolves, and temperature is down to during non-scald on hand stand-by。Take out a microscope slide, the fruit bat in step 1 fixative is pressed from both sides out gently with tweezers, being placed on microscope slide, adjustment direction is the state of lying on one's side, and drips two or three agar solution on fruit bat, repair with double-edged razor blade after agar solidification, according to fruit bat health, agar block being accomplished cuboid, cuboid is sized to three times of fruit bat volume, and fruit bat back is attached on a face of cuboid minimum area, and parallel with it, the distance of fruit bat back and cuboid agar block minimal face is 0～5 millimeter。
3. fruit bat embedded for step 2 agar sequentially passes through 0.1mol/L phosphate buffer clean 3 times, clean 10 minutes every time；2 hours are fixed with the osmic acid that mass fraction is 1%；With the Gradient elution using ethanol that volume fraction is 50%, 70%, 90%, each 1 time, the time is respectively 10 minutes；With the acetone volume ratio 1:1 dehydration 10 minutes of the ethanol of volume fraction 90% and volume fraction 90%；With pure acetone dehydration 3 times, the time is respectively 10 minutes；Permeate 1 hour with pure acetone volume ratio 1:1 with epoxy resin 618 embedding medium, permeate 3 hours with pure acetone volume ratio 3:1 with epoxy resin 618 embedding medium；Permeate overnight with pure embedding medium；Pure embedding medium embeds, and during embedding, one side parallel for fruit bat back withstands on the top of embedding plate module, and namely fruit bat back is parallel with the top end face of embedding plate module；In polymerization case, infiltration 24 hours under 36 DEG C of conditions, are polymerized 12 hours under 45 DEG C of conditions, be polymerized 48h and obtain the transmission electron microscope embedded block of agar embedding fruit bat under 65 DEG C of conditions。
Described embedding medium:
Epoxy resin 618 embedding medium formula:
Polymerizing condition: permeate 12～24 hours under 36 DEG C of conditions, is polymerized 12～48 hours under 45 DEG C of conditions, is polymerized 24～48 hours under 65 DEG C of conditions。
Epon-812 embedding medium formula:
Polymerizing condition: be polymerized 24 hours under 60 DEG C of conditions。
Spurr resin embedding agent prescription:
Polymerizing condition: be polymerized 12 hours under 70 DEG C of conditions。
4. obtain the transmission electron microscope embedded block of agar embedding fruit bat from step 3, it has been found that the position consistency put when the position of fruit bat is with resin embedding, namely back is close to embedded block top or parallel with top。Microtome cuts out the semithin section of 2 μ m thick, clean microscope slide drips distilled water, carefully semithin section is clipped on water droplet with tweezers, be placed on electric boiling plate and heat dry, drip the toluidine blue dye liquor that mass fraction is 1% again, dyeing 30 seconds, distilled water rinses, and electric boiling plate is dried, basis of microscopic observation, obtaining longitudinal tangent plane of fruit bat muscle of back, complete location, the ultrathin section for this longitudinal direction muscle position is ready。
The invention has the beneficial effects as follows: by the present invention in that embedding fruit bat with agar completes the localization method of fruit bat muscle of back longitudinal direction tangent plane, compared with conventional fruit bat sample process simpler, accurate and effective, save manpower and time, multiple repetitions of sample can be processed simultaneously。The method according to the invention, we can be widely applied to any sample needing specific part to position in electron microscopy field。
Accompanying drawing explanation
Fig. 1 is the schematic top plan view that agar of the present invention embedding fruit bat is trimmed to cuboid。
Fig. 2 is schematic diagram after fruit bat of the present invention is polymerized in embedding plate。
Fig. 3 is semithin section of the present invention, Toluidine blue staining result。
Detailed description of the invention
Fruit bat is first quickly decaptitated by the present invention, lose the glutaraldehyde fixative into volume fraction 3% and fix 2 hours, then take out and be placed on microscope slide, it is adjusted to the state of lying on one's side, drip the agar powder solution of mark 2% of improving quality, after agar solidification, be trimmed to cuboid with double-edged razor blade, be sized to three times of fruit bat, back is attached on a face of cuboid minimum area, and parallel with it；Then the osmic acid secondary of volume fraction 1% is fixed；Graded ethanol, acetone dehydration；Embedding medium gradient penetration and the embedding of pure embedding medium, during embedding, fruit bat back is pressed close to and parallel face withstands on embedding plate module foremost；It is finally putting into polymerization case polymerization；Taking out embedded block after polymerization embedding plate, microtome cuts out the semithin section of 2 μm, the Toluidine blue staining of mass fraction 1%, obtains longitudinal tangent plane of fruit bat muscle of back。This method is simple and convenient。
Embodiment 1
Required instrument comes card UC7 ultramicrotome, LIFEEVOSFLAuto full-automatic cell imaging system, microwave oven, fume hood, embedding polymerization case。
Other material required has 2ml round bottom centrifuge tube, disposable 5ml plastic suction pipe, double-edged razor blade, diamant, embedding plate, common microscope slide。
Required solution has ether, 3% glutaraldehyde solution, 2% agar powder solution, 1% osmic acid, 0.1mol/L phosphate buffer (pH value 7.2), dehydrated alcohol, and anhydrous propanone, epoxy resin 618 embedding medium is a set of, 1% toluidine blue。
Preparation 0.1mol/L phosphate buffer: A liquid, weighs 71.64gNa2HPO4·12H2O dissolves in 1000ml distilled water；B liquid, weighs 31.21gNaH2PO4·2H2O dissolves in 1000ml distilled water；Weigh the B liquid mixing of the A liquid of 36ml and 14ml, add distilled water to 100ml。4 DEG C of preservations。
The glutaraldehyde solution of preparation 3%: weigh the A liquid of 40.5ml, the B liquid of 9.5ml and the glutaraldehyde solution of 12ml25% and mix, add distilled water to 100ml, 4 DEG C of preservations。
Prepare 2% agar powder solution: weighing 1g agar powder and add in 49g distilled water, microwave-oven-heating dissolves, 4 DEG C of preservations。
Prepare 1% osmic acid: weigh 99g distilled water, load in the brown, wide-mouth bottle of 200ml, the ampere bottle that 1g osmic acid fills is knocked out tubule place, throws away in distilled water rapidly and fully dissolve, airtight preservation in glass jar。This operation carries out in fume hood。
Preparation graded ethanol: weigh 50ml, 70ml, 90ml dehydrated alcohol respectively and be separately added into 50ml, 30ml, 10ml distilled water and be made into 50%, 70%, 90% ethanol。
Prepare 90% acetone: weigh 90ml acetone and add 10ml distilled water。
Preparation embedding medium: weighing 12ml epoxy resin 618,8mlDDSA, 0.9mlDBP, 7 DMP-30 fully stir evenly。
Prepare 1% toluidine blue dye liquor: weigh in the 0.1mol/L phosphate buffer of 0.5g toluidine blue addition 49.5g and dissolve。
The method for preparing transmission electron microscopy of agar embedding fruit bat, operating procedure is as follows:
(1) fruit bat is taken out after ether is smoked and swooned, and with double-edged razor blade, head quickly cuts (artificial tear fracture head portion), is lost by fruit bat immediately in the glutaraldehyde fixative that volume fraction is 3%, and evacuation makes it sink at the bottom of pipe, fixes 2 hours for 4 DEG C。
(2) preparing the agar powder solution that mass fraction is 2% in advance, put into 4 DEG C of Refrigerator stores, used time microwave-oven-heating dissolves, and temperature is down to during non-scald on hand stand-by。Take out a microscope slide, the fruit bat in fixative is pressed from both sides out gently with tweezers, being placed on microscope slide, adjustment direction is the state of lying on one's side, and drips two or three agar solution on fruit bat, repair with double-edged razor blade after agar solidification, according to fruit bat health, agar block being accomplished cuboid, cuboid is sized to three times of fruit bat volume, and fruit bat back is attached on a face of cuboid minimum area, and it is parallel with it as it is shown in figure 1, the distance of fruit bat back and cuboid agar block minimal face is 0～5 millimeter。
(3) fruit bat embedded for step (2) agar sequentially passes through 0.1mol/L phosphate buffer clean 3 times, clean 10 minutes every time；2 hours are fixed with the osmic acid that mass fraction is 1%；With the Gradient elution using ethanol that volume fraction is 50%, 70%, 90%, each 1 time, the time is respectively 10 minutes；With the acetone volume ratio 1:1 dehydration 10 minutes of the ethanol of volume fraction 90% and volume fraction 90%；With pure acetone dehydration 3 times, the time is respectively 10 minutes；Permeate 1 hour with pure acetone volume ratio 1:1 with epoxy resin 618 embedding medium, permeate 3 hours with pure acetone volume ratio 3:1 with epoxy resin 618 embedding medium；Permeate overnight with pure embedding medium；Pure embedding medium embeds, and during embedding, one side parallel for fruit bat back withstands on the top of embedding plate module, and namely fruit bat back is parallel with the top end face of embedding plate module；In polymerization case, infiltration 24 hours under 36 DEG C of conditions, are polymerized 12 hours under 45 DEG C of conditions, be polymerized 48h and obtain the transmission electron microscope embedded block of agar embedding fruit bat as shown in Figure 2 under 65 DEG C of conditions。
(4) the transmission electron microscope embedded block of agar embedding fruit bat is obtained from step (3), it has been found that the position consistency put when the position of fruit bat is with resin embedding, namely back is close to embedded block top or parallel with top。Microtome cuts out the semithin section of 2 μ m thick, clean microscope slide drips distilled water, carefully semithin section is clipped on water droplet with tweezers, be placed on electric boiling plate and heat dry, drip the toluidine blue dye liquor that mass fraction is 1% again, dyeing 30 seconds, distilled water rinses, and electric boiling plate is dried, basis of microscopic observation, obtaining longitudinal tangent plane of fruit bat muscle of back as it is shown on figure 3, complete location, the ultrathin section for this longitudinal direction muscle position is ready。
When conventional method generally processes multiple repetition, being finally likely to only one of which embedded block back position desirable, most fruit bats are waftd in the course of the polymerization process from original position, even waft to the centre of resin mass and surface, and the back position that we want changes direction。In this case, or the angle of rotation diamant, allow the edge of a knife to coordinate the direction at fruit bat back, but provide inconvenience for follow-up ultrathin section；Being cut off from resin mass by sample, readjust position and carry out secondary, three embeddings, both processing methods all waste time and energy, loaded down with trivial details, efficiency is low。The present invention use agar embed the method that fruit bat realizes the location of back longitudinal direction muscle in advance, compared with the conventional Electronic Speculum processing method of fruit bat sample, simple to operate, efficiency is high。
Claims (1)
1. the method for preparing transmission electron microscopy of agar embedding fruit bat, is the method preparing transmission electron microscope sample with agar solution after being embedded in advance by fruit bat to realize quickly positioning fruit bat muscle of back, it is characterised in that: operating procedure is as follows:
(1) fruit bat is taken out after ether is smoked and swooned, with double-edged razor blade, quickly head is cut (inartificial tear fracture head portion), being lost by fruit bat immediately in the glutaraldehyde fixative that volume fraction is 3%, evacuation makes it sink at the bottom of pipe, fixes 2 hours under 4 DEG C of conditions；
(2) the agar powder solution that mass fraction is 2% is prepared in advance, put into 4 DEG C of Refrigerator stores, used time microwave-oven-heating dissolves, temperature is down to during non-scald on hand stand-by, take out a microscope slide, the fruit bat in step (1) fixative is pressed from both sides out gently with tweezers, it is placed on microscope slide, adjustment direction is the state of lying on one's side, fruit bat is dripped two or three agar solution, repair with double-edged razor blade after agar solidification, according to fruit bat health, agar block is accomplished cuboid, cuboid is sized to three times of fruit bat volume, fruit bat back is attached on a face of cuboid minimum area, and it is parallel with it, the distance of fruit bat back and cuboid agar block minimal face is 0～5 millimeter；
(3) fruit bat embedded for step (2) agar sequentially passes through 0.1mol/L phosphate buffer clean 3 times, clean 10 minutes every time；2 hours are fixed with the osmic acid that mass fraction is 1%；With the Gradient elution using ethanol that volume fraction is 50%, 70%, 90%, each 1 time, the time is respectively 10 minutes；With the acetone volume ratio 1:1 dehydration 10 minutes of the ethanol of volume fraction 90% and volume fraction 90%；With pure acetone dehydration 3 times, the time is respectively 10 minutes；Permeate 1 hour with pure acetone volume ratio 1:1 with epoxy resin 618 embedding medium, permeate 3 hours with pure acetone volume ratio 3:1 with epoxy resin 618 embedding medium；Permeate overnight with pure embedding medium；Pure embedding medium embeds, and during embedding, one side parallel for fruit bat back withstands on the top of embedding plate module, and namely fruit bat back is parallel with the top end face of embedding plate module；In polymerization case, infiltration 24 hours under 36 DEG C of conditions, are polymerized 12 hours under 45 DEG C of conditions, and under 65 DEG C of conditions, polymerization obtains the transmission electron microscope embedded block of agar embedding fruit bat for 48 hours；
Described embedding medium:
Epoxy resin 618 embedding medium formula:
Polymerizing condition: permeate 12～24 hours under 36 DEG C of conditions, is polymerized 12～48 hours under 45 DEG C of conditions, is polymerized 24～48 hours under 65 DEG C of conditions；
Epon-812 embedding medium formula:
Polymerizing condition: be polymerized 24 hours under 60 DEG C of conditions；
Spurr resin embedding agent prescription:
Polymerizing condition: be polymerized 12 hours under 70 DEG C of conditions；
(4) the transmission electron microscope embedded block of agar embedding fruit bat is obtained from step (3), it has been found that the position consistency put when the position of fruit bat is with resin embedding, namely back is close to embedded block top or parallel with top。Microtome cuts out the semithin section of 2 μ m thick, clean microscope slide drips distilled water, carefully semithin section is clipped on water droplet with tweezers, be placed on electric boiling plate and heat dry, drip the toluidine blue dye liquor that mass fraction is 1% again, dyeing 30 seconds, distilled water rinses, and electric boiling plate is dried, basis of microscopic observation, obtaining longitudinal tangent plane of fruit bat muscle of back, complete location, the ultrathin section for this longitudinal direction muscle position is ready。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610103594.8A CN105699145A (en) | 2016-02-25 | 2016-02-25 | Method for preparing transmission electron microscope sample by embedding drosophila melanogaster in agar |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610103594.8A CN105699145A (en) | 2016-02-25 | 2016-02-25 | Method for preparing transmission electron microscope sample by embedding drosophila melanogaster in agar |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105699145A true CN105699145A (en) | 2016-06-22 |
Family
ID=56223170
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610103594.8A Pending CN105699145A (en) | 2016-02-25 | 2016-02-25 | Method for preparing transmission electron microscope sample by embedding drosophila melanogaster in agar |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105699145A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106442529A (en) * | 2016-09-26 | 2017-02-22 | 华南农业大学 | Fresh tobacco leaf slices and making method and application thereof |
| CN109141997A (en) * | 2018-08-03 | 2019-01-04 | 福建农林大学 | A kind of flaking method of microexamination Aphytis |
| CN113686643A (en) * | 2021-08-23 | 2021-11-23 | 中国人民解放军陆军特色医学中心 | Embedding kit for paraffin section of organoid tissue and preparation method of paraffin section |
| CN114459851A (en) * | 2021-12-28 | 2022-05-10 | 苏州药明检测检验有限责任公司 | Method for detecting viruses in UPB (ultra-thin sheet) by using ultrathin section electron microscope technology |
| CN117554150A (en) * | 2023-11-21 | 2024-02-13 | 中国科学院南京地质古生物研究所 | Directional embedding method and detection method for radiofossa |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD129238A1 (en) * | 1976-11-22 | 1978-01-04 | Peter Deicke | METHOD FOR PREPARING FINE-CONCERNED FABRICS FOR HISTOLOGICAL STUDIES |
| CN1749730A (en) * | 2004-09-17 | 2006-03-22 | 中国科学院海洋研究所 | A kind of tissue section method of seawater left-eyed flounder zygote |
| CN104977202A (en) * | 2015-08-07 | 2015-10-14 | 广西医科大学 | Preparation method of transmission electron microscope sample of paraffin-embedded section tissue |
| CN105258999A (en) * | 2015-11-06 | 2016-01-20 | 中南大学湘雅医院 | Single-cell transmission electron microscope sample preparation method |
-
2016
- 2016-02-25 CN CN201610103594.8A patent/CN105699145A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD129238A1 (en) * | 1976-11-22 | 1978-01-04 | Peter Deicke | METHOD FOR PREPARING FINE-CONCERNED FABRICS FOR HISTOLOGICAL STUDIES |
| CN1749730A (en) * | 2004-09-17 | 2006-03-22 | 中国科学院海洋研究所 | A kind of tissue section method of seawater left-eyed flounder zygote |
| CN104977202A (en) * | 2015-08-07 | 2015-10-14 | 广西医科大学 | Preparation method of transmission electron microscope sample of paraffin-embedded section tissue |
| CN105258999A (en) * | 2015-11-06 | 2016-01-20 | 中南大学湘雅医院 | Single-cell transmission electron microscope sample preparation method |
Non-Patent Citations (3)
| Title |
|---|
| 曾晓蓓 等: "制备组织石蜡切片琼脂预包埋法的应用性探讨", 《实验技术与管理》 * |
| 浦涛 等: "果蝇胚胎石蜡切片制作方法的改良", 《生物学通报》 * |
| 辜清 等: "《人体组织学与解剖学实验》", 30 June 1999 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106442529A (en) * | 2016-09-26 | 2017-02-22 | 华南农业大学 | Fresh tobacco leaf slices and making method and application thereof |
| CN109141997A (en) * | 2018-08-03 | 2019-01-04 | 福建农林大学 | A kind of flaking method of microexamination Aphytis |
| CN113686643A (en) * | 2021-08-23 | 2021-11-23 | 中国人民解放军陆军特色医学中心 | Embedding kit for paraffin section of organoid tissue and preparation method of paraffin section |
| CN114459851A (en) * | 2021-12-28 | 2022-05-10 | 苏州药明检测检验有限责任公司 | Method for detecting viruses in UPB (ultra-thin sheet) by using ultrathin section electron microscope technology |
| CN117554150A (en) * | 2023-11-21 | 2024-02-13 | 中国科学院南京地质古生物研究所 | Directional embedding method and detection method for radiofossa |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105699145A (en) | Method for preparing transmission electron microscope sample by embedding drosophila melanogaster in agar | |
| Tizro et al. | Sample preparation for transmission electron microscopy | |
| Paiva et al. | Large plant samples: how to process for GMA embedding? | |
| US20220299407A1 (en) | Method and apparatus for faster pathology | |
| US7767434B2 (en) | Ultrasound-mediated high-speed biological reaction and tissue processing | |
| CN104977202A (en) | Preparation method of transmission electron microscope sample of paraffin-embedded section tissue | |
| Hegazy | Hegazy’simplified method of tissue processing (consuming time and chemicals) | |
| CN103558055B (en) | A kind of locating super-thin dicing method for infecting viral sick cell | |
| EP3229007B1 (en) | Method for preparing liquid-state dropping or coating pathological quality control product, and uses thereof | |
| Carrara et al. | Histological examination of the diabetic kidney | |
| US11467070B2 (en) | Porous material for the inclusion of cytologic preparations, process for obtaining the same and its use | |
| JP5274509B2 (en) | Frozen thin section preparation device | |
| Ul-Hamid et al. | Sample preparation | |
| CN109459262A (en) | A kind of preparation method that maturation maize root system is temporarily sliced | |
| JP3147841U (en) | Super easy microtome | |
| Orchard et al. | Dermatofibrosarcoma protuberans: dealing with slow Mohs procedures employing formalin-fixed, paraffin wax-embedded tissue in a busy diagnostic laboratory | |
| Sathyakumar et al. | Tissue processing of oral biopsy specimens: An adjunct to diagnosis | |
| Estrada et al. | TEM of paraffin-embedded H&E-stained sections for viral diagnosis (an unusual papovavirus case) | |
| RU2797125C1 (en) | Method for preparing bone tissue samples with implanted metal for histological study | |
| Auvinen | Manual for hard tissue research laboratory | |
| Flores et al. | Improved high resolution light microscopy and transmission electron microscopy techniques in diagnostic pathology | |
| WO1996040506A1 (en) | Method of optimizing tissue for histopathologic examination | |
| Selvamani et al. | Microwave Histotechnology Vs Conventional Histotechnology: A Review | |
| Mun et al. | 3D Microscopy of Murine Bone Marrow Hematopoietic Tissues | |
| Sanders | Recent Advances in Microwave Assisted Specimen Processing: Keeping it Cool. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160622 |
|
| WD01 | Invention patent application deemed withdrawn after publication |