CN105688765A - Novel encoding microsphere and preparation method thereof - Google Patents
Novel encoding microsphere and preparation method thereof Download PDFInfo
- Publication number
- CN105688765A CN105688765A CN201610084734.1A CN201610084734A CN105688765A CN 105688765 A CN105688765 A CN 105688765A CN 201610084734 A CN201610084734 A CN 201610084734A CN 105688765 A CN105688765 A CN 105688765A
- Authority
- CN
- China
- Prior art keywords
- microsphere
- coding
- fluorescent
- raman
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
- B01J13/02—Making microcapsules or microballoons
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
- C09K2211/10—Non-macromolecular compounds
- C09K2211/1018—Heterocyclic compounds
- C09K2211/1025—Heterocyclic compounds characterised by ligands
- C09K2211/1088—Heterocyclic compounds characterised by ligands containing oxygen as the only heteroatom
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
- C09K2211/14—Macromolecular compounds
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Materials Engineering (AREA)
- Engineering & Computer Science (AREA)
- Dispersion Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a novel encoding microsphere and a preparation method thereof and belongs to the technical field of composites.The preparation method includes: using melamine resin as a base material and using Raman spectrum and fluorescent spectrum as encoding elements, wrapping fluorescent probe molecules in situ into a melamine resin microsphere by means of sol-gel method to obtain an MF fluorescent microsphere, coating the surface of the microsphere with an Ag nanoparticle layer to form an MF/Ag-NPs composite microsphere so as to impart good biocompatibility, and preparing the novel encoding microsphere responsive to both surface enhanced Raman and fluorescence.The obtained encoding microsphere has extended encoding capacity, can greatly reduce encoding signal interference due to spectral overlap so as to enhance the stability of encoding signals, and has a good application prospect in terms of biological detection.
Description
Technical field
The invention belongs to technical field of composite materials, be specifically related to a kind of novel coding microsphere and preparation method thereof。
Background technology
Medical diagnosis on disease and treatment tool are of great significance by the detection quick, high-sensitive of the biomolecule such as antibody, antigen, DNA。But, typically require in detection process and target molecule is carried out substantial amounts of screening and analysis, accordingly, it would be desirable to suitable method tackles substantial amounts of biomarker analysis。Coding microball detection technique is target molecule to be fixed on the microsphere surface with specific coding, then the target molecule being loaded with further according to coding its surface of signal identification of coding microball, when analyzing, only need recognition coding signal, it is possible to determine corresponding testing molecule。At present, the coded system of microsphere mainly has location graphic coding and optical spectrum encoded。The molecule of various luminescences or other microgranules can the mode passed through covalently or non-covalently be combined with microsphere, thus preparing optical spectrum encoded microsphere。These luminescent substances include fluorescent colloid particle, luminescent dye molecule, semiconductor nanocrystal, rare earth element etc., and wherein with the most use is quantum dot and fluorescent dye。Quantum dot has excitation wave length and width, launches the characteristic that wavelength is narrow, therefore, is the good selection preparing fluorescence-encoded micro-beads。Although the quantum yield of quantum dot is high, but its preparation of dyestuff and modification are all more difficult relatively, and relatively costly。Fluorescent dye abundance, low price, and the fluorescence-encoded micro-beads of current bibliographical information still luminous based on organic dyestuff, as long as selecting the suitable dye just can the fluorescence-encoded micro-beads of processability excellence。Except fluorescence-encoded, Raman spectrum is also attempted and is used as coding, and for Multiple detection。
Code capacity is one of significant challenge of current encoder microsphere preparation and application。Encoding for Raman, owing to structure is similar, the Raman shift of Raman reporter molecules is generally focused on 500cm-1-2000cm-1In scope, and a kind of Raman molecular is usually constructed with several characteristic peaks。Therefore, when several Raman moleculars are used as coding together, the inevitably overlap at occurrence characteristics peak, thus causing that the capacity of coding is substantially reduced。It addition, for fluorescence-encoded micro-beads, there is also the problem that emission peak is overlapping。Such as fluorescence energy transfer etc. also tend to cause to excite, emission peak is overlapping and fluorescence signal power changes, therefore it is much fewer than theoretic to can be used for the fluorescent dye of actual coding and quantum dot, thus the signal that can be used for label coding so far still far can not practical requirement。Therefore, develop the coding microball with big code capacity and coded method has great significance。
The present invention utilizes Raman spectrum and fluorescence spectrum jointly to encode, and adopts binary mode to be encoded, thus greatly expanding the code capacity of microsphere。If being used alone two kinds of Raman moleculars or two kinds of fluorescence molecules preparing coding microball, 3 kinds of coding microballs can be obtained respectively, altogether just can obtain 6 kinds of coding microballs。And two kinds of Ramans and two kinds of fluorescence molecules are encoded jointly, 15 kinds of different dual coding microspheres of signal can be prepared through permutation and combination, say, that Raman and fluorescence combine the code capacity greatly adding coding microball。Further, it is also possible to the spectral signal intensity according to various signals is encoded, obtain more coding microball, it can be seen that, the coding microball of present invention exploitation has great application potential in Multiple detection。
Summary of the invention
It is an object of the invention to provide a kind of novel coding microsphere and preparation method thereof。Gained coding microball has big code capacity and its feature that coding signal disturbing is weak, coding signal is strong and stable, has a good application prospect in biological detection。
For achieving the above object, the present invention adopts the following technical scheme that
A kind of novel coding microsphere is employing melamine resin is base material, makes described coding microball with Raman spectrum and fluorescence spectrum collectively as code element。
The preparation method of described novel coding microsphere, is adopt sol-gel process in the preparation process of melamine resin microsphere, using hydrochloric acid as catalyst, by fluorescent probe molecule in-stiu coating in microsphere, only prepares MF fluorescent microsphere by single step reaction；Then adopt chemical precipitation method, with sodium borohydride for reducing agent, by Ag nanoparticle in-stiu coating at microsphere surface, form MF/Ag-NPs complex microsphere；After forming FMF/Ag microsphere in conjunction with Raman labels molecule, recycling supercritical ultrasonics technology is at one layer of SiO of its finishing2Shell, prepares and has a surface-enhanced Raman and coding microball that fluorescence double-bang firecracker is answered。
It specifically includes following steps:
1) 0.2 ~ 3g tripolycyanamide and 0.1 ~ 2g formaldehyde are joined in the deionized water of 100mL, it is 4 with hydrochloric acid regulation system pH value, it is subsequently adding 5-80mg fluorescent probe molecule, stand after reaction 2h, removing upper strata emulsion, the products in water obtained and ethanol are washed three times respectively, and centrifugation goes out product, then in 50 DEG C of vacuum dryings, size uniformity, monodispersed MF fluorescent microsphere are obtained；
2) MF fluorescent microsphere step 1) obtained joins 20mL containing AgNO3In the alcoholic solution of 2-10mg, add 2-15mg sodium borohydride, then by mixture supersound process 2min, be then placed in shaking table, oscillating reactions 60min at 50 DEG C, use ethanol purge 2-3 time after centrifugal, 50 DEG C of vacuum drying 2h, obtain MF/Ag-NPs complex microsphere；
3) by 1-20mg step 2) to be dispersed in Raman labels molecule content be 10 for the MF/Ag-NPs complex microsphere that obtains-3In the alcoholic solution of mol/L, put into and shaking table at room temperature reacts 1.5h, obtain the FMF/Ag microsphere of Raman labels molecular modification；
4) FMF/Ag microsphere step 3) obtained joins in the mixed solution containing 20-100mL ethanol, 6-30mL deionized water, 0.5-4mL ammonia and 0.1-2mLTEOS, 1.0h is reacted under ul-trasonic irradiation, with ethanol and washing successively washing 3 times after product centrifugation, obtain described novel coding microsphere。
Described fluorescent probe molecule is any one or two kinds in FITC, PHB；
Described Raman labels molecule be p-aminophenyl thiophenol (ABT), to chloro phenylmercaptan. (CBT), thiohydroquinone (HBT) and 5,5-dithio double; two-(2-Nitrobenzol hydrochloric acid) (DTNB) in any one or two kinds。
The present invention has the great advantage that
(1) fluorescent probe molecule and Raman labels molecule are separately fixed at the different shells of microsphere by the present invention, it is possible to greatly reduce the coding signal disturbing caused because of spectra overlapping, strengthen stablizing of coding signal simultaneously；
(2) present invention adopts the mode that two kinds of code elements are encoded to considerably increase the code capacity of microsphere, it is adaptable to the detection of a large amount of target molecules。
(3) present invention is using hydrochloric acid as catalyst, and a step prepares MF fluorescent microsphere, enhances the microsphere coating function to fluorescent probe molecule so that fluorescence molecule is not easily revealed, and improves stability and the preparation efficiency of fluorescent microsphere。
(4) present invention is using sodium borohydride as reducing agent, shortens the response time；And adopt supercritical ultrasonics technology to replace mechanical mixing method coated Si O2Shell, makes this reaction required time shorten to 1h。
Accompanying drawing explanation
Fig. 1 is the shape appearance figure of each microsphere of gained in embodiment 1 preparation process, the SEM scanning figure that wherein (a) is MF fluorescent microsphere；(b is the fluorescence microscopy figure of MF fluorescent microsphere；C SEM scanning figure that () is MF/Ag-NPs microsphere；D SEM scanning figure that () is coding microball。
Fig. 2 is surface enhanced Raman spectroscopic signals, fluorescence spectrum signal and the binary coded signal figure that 15 kinds of coding microballs are corresponding。
Detailed description of the invention
In order to make content of the present invention easily facilitate understanding, below in conjunction with detailed description of the invention, technical solutions according to the invention are described further, but the present invention is not limited only to this。
Embodiment 1:There is a kind of Raman and the coding microball of a kind of fluorescence-encoded signal
What it was prepared specifically comprises the following steps that
1) 0.2g tripolycyanamide and 0.6g formaldehyde are joined in the deionized water of 100mL, it is 4 with hydrochloric acid regulation system pH value, it is separately added into fluorescent probe molecule and is subsequently adding 5mg fluorescent probe molecule FITC, standing after reaction 2h, remove upper strata emulsion, the products in water obtained and ethanol wash three times respectively, centrifugation goes out product, then in 50 DEG C of vacuum dryings, obtaining size uniformity, monodispersed MF fluorescent microsphere, its SEM scanning figure and fluorescence microscopy figure is shown in Fig. 1 (a) and Fig. 1 (b) respectively；
2) MF fluorescent microsphere step 1) obtained joins 20mL containing AgNO3In the alcoholic solution of 2mg, add 2mg sodium borohydride, then by mixture supersound process 2min, it is then placed in shaking table, oscillating reactions 60min at 50 DEG C, uses ethanol purge 2 times after centrifugal, 50 DEG C of vacuum drying 2h, obtain MF/Ag-NPs complex microsphere, and its SEM scanning figure is shown in Fig. 1 (c)；
3) by 5mg step 2) the MF/Ag-NPs complex microsphere that obtains is dispersed in chlorothio-phenol (CBT) content is 10-3In the alcoholic solution of mol/L, put into and shaking table at room temperature reacts 1.5h, obtain the FMF/Ag microsphere of Raman labels molecular modification；
4) FMF/Ag microsphere step 3) obtained joins in the mixed solution containing 20mL ethanol, 6mL deionized water, 0.5mL ammonia and 0.1mLTEOS, 1.0h is reacted under ul-trasonic irradiation, with ethanol and washing successively washing 3 times after product centrifugation, obtain Raman-fluorescence dual coding microsphere。
Its SEM scanning figure is shown in Fig. 1 (d)。
Prepared dual coding microsphere being carried out Raman and fluoroscopic examination, and adopts binary mode to be encoded, it is encoded to 1010。
Embodiment 2:There is a kind of Raman and the coding microball of two kinds of fluorescence-encoded signals
1) 0.625g tripolycyanamide and 0.9g formaldehyde are joined in the deionized water of 100mL, it is 4 with hydrochloric acid regulation system pH value, it is separately added into 30mg fluorescent probe molecule FITC and PHB, stand after reaction 2h, removing upper strata emulsion, the products in water obtained and ethanol are washed three times respectively, and centrifugation goes out product, then in 50 DEG C of vacuum dryings, size uniformity, monodispersed MF fluorescent microsphere are obtained；
2) MF fluorescent microsphere step 1) obtained joins 20mL containing AgNO3In the alcoholic solution of 8mg, add 8mg sodium borohydride, then by mixture supersound process 2min, be then placed in shaking table, oscillating reactions 60min at 50 DEG C, use ethanol purge 2 times after centrifugal, 50 DEG C of vacuum drying 2h, obtain MF/Ag-NPs complex microsphere；
3) by 10mg step 2) to be dispersed in p-aminophenyl thiophenol (ABT) content be 10 for the MF/Ag-NPs complex microsphere that obtains-3In the alcoholic solution of mol/L, put into and shaking table at room temperature reacts 1.5h, obtain the FMF/Ag microsphere of Raman labels molecular modification；
4) FMF/Ag microsphere step 3) obtained joins in the mixed solution containing 40mL ethanol, 18mL deionized water, 2mL ammonia and 1.0mLTEOS, 1.0h is reacted under ul-trasonic irradiation, with ethanol and washing successively washing 3 times after product centrifugation, obtain Raman-fluorescence dual coding microsphere。
Prepared dual coding microsphere being carried out Raman and fluoroscopic examination, and adopts binary mode to be encoded, it is encoded to 1011。
Embodiment 3:There is the dual coding microsphere of two kinds of Ramans and two kinds of fluorescence-encoded signals
What it was prepared specifically comprises the following steps that
1) 3g tripolycyanamide and 2g formaldehyde are joined in the deionized water of 100mL, it is 4 with hydrochloric acid regulation system pH value, it is separately added into 40mg fluorescent probe molecule FITC and PHB, stand after reaction 2h, removing upper strata emulsion, the products in water obtained and ethanol are washed three times respectively, and centrifugation goes out product, then in 50 DEG C of vacuum dryings, size uniformity, monodispersed MF fluorescent microsphere are obtained；
2) MF fluorescent microsphere step 1) obtained joins 20mL containing AgNO3In the alcoholic solution of 10mg, add 15mg sodium borohydride, then by mixture supersound process 2min, be then placed in shaking table, oscillating reactions 60min at 50 DEG C, use ethanol purge 3 times after centrifugal, 50 DEG C of vacuum drying 2h, obtain MF/Ag-NPs complex microsphere；
3) by 20mg step 2) the MF/Ag-NPs complex microsphere that obtains is dispersed in and chlorothio-phenol (CBT) and thiohydroquinone (HBT) content is 10-3In the alcoholic solution of mol/L, put into and shaking table at room temperature reacts 1.5h, obtain the FMF/Ag microsphere of Raman labels molecular modification；
4) FMF/Ag microsphere step 3) obtained joins in the mixed solution containing 100mL ethanol, 30mL deionized water, 4mL ammonia and 2.0mLTEOS, 1.0h is reacted under ul-trasonic irradiation, with ethanol and washing successively washing 3 times after product centrifugation, namely obtain novel Raman-fluorescence dual coding microsphere。
Prepared dual coding microsphere being carried out Raman and fluoroscopic examination, and adopts binary mode to be encoded, it is encoded to 1111。
Claims (4)
1. a novel coding microsphere, it is characterised in that: with melamine resin for base material, adopt Raman spectrum and fluorescence spectrum to make described coding microball collectively as code element。
2. the preparation method of a novel coding microsphere as claimed in claim 1, it is characterised in that: adopt sol-gel process in the preparation process of melamine resin microsphere, using hydrochloric acid as catalyst, fluorescent probe molecule in-stiu coating is formed in microsphere MF fluorescent microsphere；Then adopt chemical precipitation method, with sodium borohydride for reducing agent, by Ag nanoparticle in-stiu coating at microsphere surface, form MF/Ag-NPs complex microsphere；After forming FMF/Ag microsphere in conjunction with Raman labels molecule, then adopt supercritical ultrasonics technology at one layer of SiO of its finishing2Shell, prepares and has a surface-enhanced Raman and coding microball that fluorescence double-bang firecracker is answered。
3. the preparation method of novel coding microsphere according to claim 2, it is characterised in that: specifically include following steps:
1) 0.2 ~ 3g tripolycyanamide and 0.1-2g formaldehyde are joined in the deionized water of 100mL, it is 4 with hydrochloric acid regulation system pH value, it is subsequently adding 5-80mg fluorescent probe molecule, after reaction 2h, the products in water obtained and ethanol are washed three times respectively, centrifugation goes out product, then in 50 DEG C of vacuum dryings, size uniformity, monodispersed MF fluorescent microsphere are obtained；
2) MF fluorescent microsphere step 1) obtained joins 20mL containing AgNO3In the alcoholic solution of 2-10mg, add 2-15mg sodium borohydride, then by mixture supersound process 2min, be then placed in shaking table, oscillating reactions 60min at 50 DEG C, use ethanol purge 2-3 time after centrifugal, 50 DEG C of vacuum drying 2h, obtain MF/Ag-NPs complex microsphere；
3) by 1-20mg step 2) to be dispersed in Raman labels molecule content be 10 for the MF/Ag-NPs complex microsphere that obtains-3In the alcoholic solution of mol/L, put into and shaking table at room temperature reacts 1.5h, obtain the FMF/Ag microsphere of Raman labels molecular modification；
4) the FMF/Ag microsphere surface that supercritical ultrasonics technology obtains in step 3) is adopted to modify SiO2Shell, obtains described novel coding microsphere。
4. the preparation method of novel coding microsphere according to Claims 2 or 3, it is characterised in that: described fluorescent probe molecule is any one or two kinds in FITC, PHB；
Described Raman labels molecule be p-aminophenyl thiophenol, to chloro phenylmercaptan., thiohydroquinone, 5,5-dithio double; two-(2-Nitrobenzol hydrochloric acid) in any one or two kinds。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610084734.1A CN105688765A (en) | 2016-02-15 | 2016-02-15 | Novel encoding microsphere and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610084734.1A CN105688765A (en) | 2016-02-15 | 2016-02-15 | Novel encoding microsphere and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105688765A true CN105688765A (en) | 2016-06-22 |
Family
ID=56222995
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610084734.1A Pending CN105688765A (en) | 2016-02-15 | 2016-02-15 | Novel encoding microsphere and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105688765A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107674669A (en) * | 2017-09-26 | 2018-02-09 | 福州大学 | A kind of macromolecule composite coding microballoon and preparation method thereof |
| CN110760301A (en) * | 2018-07-26 | 2020-02-07 | 合皓股份有限公司 | Siloxane organic fluorescent powder and preparation method thereof |
| CN110893334A (en) * | 2018-09-12 | 2020-03-20 | 福州大学 | Polyphosphazene fluorescence-surface enhanced Raman coding microsphere and preparation method thereof |
| CN111206081A (en) * | 2018-11-21 | 2020-05-29 | 思纳福(北京)医疗科技有限公司 | Nucleic acid detection microsphere, preparation method, kit and high-throughput nucleic acid detection method |
| CN113281317A (en) * | 2021-05-14 | 2021-08-20 | 北京指真生物科技有限公司 | Coded microsphere containing cyanine compounds, and preparation method and application thereof |
| CN114414546A (en) * | 2022-01-28 | 2022-04-29 | 福州大学 | High-flux liquid-phase biomolecule detection method and device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102323412A (en) * | 2011-08-09 | 2012-01-18 | 中国科学院合肥物质科学研究院 | A kind of purposes of Raman coding microball and utilize the Raman coding microball to detect the method for tumor markers |
| CN102608099A (en) * | 2012-02-29 | 2012-07-25 | 哈尔滨工业大学 | Method for preparing surface-enhanced Raman spectroscopy substrate of silver self-assembly under assistance of amino acids |
| WO2012071428A3 (en) * | 2010-11-22 | 2012-08-02 | Solulink, Inc. | Methods and/or use of oligonucleotide conjugates for assays and detections |
| CN103645172A (en) * | 2013-12-13 | 2014-03-19 | 江南大学 | Spherical surface enhanced Raman scattering (SERS) active substrate and preparation method thereof |
| US20140170698A1 (en) * | 2009-05-07 | 2014-06-19 | Nodality, Inc. | Microbead Kit and Method for Quantitative Calibration and Performance Monitoring of a Fluorescence Instrument |
-
2016
- 2016-02-15 CN CN201610084734.1A patent/CN105688765A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140170698A1 (en) * | 2009-05-07 | 2014-06-19 | Nodality, Inc. | Microbead Kit and Method for Quantitative Calibration and Performance Monitoring of a Fluorescence Instrument |
| WO2012071428A3 (en) * | 2010-11-22 | 2012-08-02 | Solulink, Inc. | Methods and/or use of oligonucleotide conjugates for assays and detections |
| CN102323412A (en) * | 2011-08-09 | 2012-01-18 | 中国科学院合肥物质科学研究院 | A kind of purposes of Raman coding microball and utilize the Raman coding microball to detect the method for tumor markers |
| CN102608099A (en) * | 2012-02-29 | 2012-07-25 | 哈尔滨工业大学 | Method for preparing surface-enhanced Raman spectroscopy substrate of silver self-assembly under assistance of amino acids |
| CN103645172A (en) * | 2013-12-13 | 2014-03-19 | 江南大学 | Spherical surface enhanced Raman scattering (SERS) active substrate and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 游力军: "基于酚醛和密胺树脂的新型核壳结构复合微球的制备与应用研究", 《中国博士学位论文全文数据库 工程科技Ⅰ辑》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107674669A (en) * | 2017-09-26 | 2018-02-09 | 福州大学 | A kind of macromolecule composite coding microballoon and preparation method thereof |
| CN110760301A (en) * | 2018-07-26 | 2020-02-07 | 合皓股份有限公司 | Siloxane organic fluorescent powder and preparation method thereof |
| CN110893334A (en) * | 2018-09-12 | 2020-03-20 | 福州大学 | Polyphosphazene fluorescence-surface enhanced Raman coding microsphere and preparation method thereof |
| CN111206081A (en) * | 2018-11-21 | 2020-05-29 | 思纳福(北京)医疗科技有限公司 | Nucleic acid detection microsphere, preparation method, kit and high-throughput nucleic acid detection method |
| CN113281317A (en) * | 2021-05-14 | 2021-08-20 | 北京指真生物科技有限公司 | Coded microsphere containing cyanine compounds, and preparation method and application thereof |
| CN114414546A (en) * | 2022-01-28 | 2022-04-29 | 福州大学 | High-flux liquid-phase biomolecule detection method and device |
| CN114414546B (en) * | 2022-01-28 | 2023-07-28 | 福州大学 | High-flux liquid-phase biomolecule detection method and device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105688765A (en) | Novel encoding microsphere and preparation method thereof | |
| Fan et al. | Optical multiplexed bioassays for improved biomedical diagnostics | |
| JP4786096B2 (en) | Surface-enhanced spectroscopy-active composite nanoparticles | |
| Vo‐Dinh et al. | SERS nanosensors and nanoreporters: golden opportunities in biomedical applications | |
| Hennig et al. | Scope and limitations of surface functional group quantification methods: exploratory study with poly (acrylic acid)-grafted micro-and nanoparticles | |
| Capehart et al. | Controlled integration of gold nanoparticles and organic fluorophores using synthetically modified MS2 viral capsids | |
| US7443489B2 (en) | Surface enhanced spectroscopy-active composite nanoparticles | |
| Guo et al. | Conformational details of quantum dot-DNA resolved by forster resonance energy transfer lifetime nanoruler | |
| CN1767941A (en) | Fluorescent silica-based nanoparticles | |
| CN104926981B (en) | A kind of preparation method based on molecular engram carbon point fluorescent sensing material | |
| CN106867509A (en) | A kind of Nd3+Conversion nano crystalline substance material and preparation method thereof and water detect application on sensitization nucleocapsid | |
| CN104907019A (en) | Magnetic fluorescent Raman double-encoding composite microspheres and preparation method and application thereof | |
| CN102721680A (en) | Method for high-sensitivity detection for t-DNA (transfer-deoxyribose nucleic acid) by aid of SERS (surface enhanced Raman spectroscopy) liquid chip | |
| WO2008001978A1 (en) | Surface enhanced raman scattering nano-tagging particle and method for preparing thereof | |
| JP2005534931A (en) | Combinatorial analysis library and colored coded laminated microcapsules as specific optical sensors | |
| CN107238709A (en) | AFP detection methods based on FRET | |
| Zhu et al. | Luminescence amplification strategies integrated with microparticle and nanoparticle platforms | |
| CN107238708A (en) | A kind of method based on the homogeneous immune detection CEA of quantum dot | |
| CN103091300B (en) | TNT (Trinitrotoluene) detection method based on surface-enhanced resonance Raman scattering | |
| CN103234951A (en) | Preparation method for precious metal nanoparticle-cladded photonic crystal-encoded microsphere | |
| CN109504366A (en) | A kind of rare-earth complex cladding nano-hollow SiO2With cladded type rare-earth complex and preparation method thereof | |
| CN107674669A (en) | A kind of macromolecule composite coding microballoon and preparation method thereof | |
| CN101824477A (en) | Substrate, gene chip and preparation method thereof and target detection method | |
| You et al. | Synthesis of high quality dual-mode encoded phenolic resin microspheres based on Fluorescence and Surface Enhanced Raman Spectra | |
| Liu et al. | Preparation of fluorescence-encoded microspheres based on hydrophobic conjugated polymer–dye combination and the immunoassay |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160622 |