CN105688721A - Micro-fluidic chip for generating spherical microbubbles - Google Patents

Micro-fluidic chip for generating spherical microbubbles Download PDF

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CN105688721A
CN105688721A CN201610002404.3A CN201610002404A CN105688721A CN 105688721 A CN105688721 A CN 105688721A CN 201610002404 A CN201610002404 A CN 201610002404A CN 105688721 A CN105688721 A CN 105688721A
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microbubble
chip
microchannel
micro
gas
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CN105688721B (en
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包福兵
项素娟
凃程旭
林建忠
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China Jiliang University
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    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/3034—Micromixers using induced convection or movement in the mixture to mix or move the fluids without mechanical means, e.g. thermodynamic instability, strong gradients, etc.
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/301—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions
    • B01F33/3012—Interdigital streams, e.g. lamellae
    • B01F33/30121—Interdigital streams, e.g. lamellae the interdigital streams being concentric lamellae
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
    • B01F33/3022—Micromixers the materials to be mixed flowing in the form of droplets the components being formed by independent droplets which are alternated, the mixing of the components being achieved by diffusion between droplets
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
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    • B01L2200/0636—Focussing flows, e.g. to laminate flows
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    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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Abstract

The invention discloses a micro-fluidic chip for generating spherical microbubbles.The micro-fluidic chip is formed by bonding two chip bodies which are different in internal microchannel structure, and microchannels with certain thickness and width are etched in the bonding surface of each chip body through a photoetching technology.The first chip body is internally provided with two liquid microchannels, a gas microchannel, a microbubble generation channel and a microbubble output channel, and an included angle with a certain angle exists on the junction of each liquid microchannel and the gas microchannel; the second chip body is internally provided with liquid microchannels, a gas microchannel and a microbubble output channel which all have the same structural sizes as those of the first chip body, but the microbubble generation channel does not exist in the second chip body.After the two chip bodies are bonded to form the micro-fluidic chip, liquid and gas are injected into the microchannels simultaneously, and the microbubbles with different diameter sizes are generated by adjusting the liquid flow and controlling the gas pressure.According to the micro-fluidic chip for generating the spherical microbubbles, the limitation of the microbubbles generated by a micro-fluidic element on the aspects of structure and shape is broken through.

Description

For generating the micro-fluidic chip of spherical microbubble
Technical field
The present invention relates to a kind of micro-fluidic chip, particularly relate to a kind of micro-fluidic chip generating spherical micron dimension bubble, belong to field of fluid machinery。
Background technology
In recent years, micron dimension bubble, because of its unique fluid mechanical characteristic having and scale effect, is applied widely in fields such as medical science, oil, environment, mining, chemical industry, power, metallurgy, nuclear energy。Especially at biomedical sector, various obtain develop rapidly based on the ultrasonic measurement technology of microbubble, targeted drug delivery and gene mediated treatment technology。The microbubble size of generation, stability etc. all can affect its effect in every field application。Therefore, further investigate the formation mechenism of microbubble and flow behavior, it is possible to establish microbubble multi-field practical application theoretical basis。
Along with the Chinese scholars further investigation to microbubble, occur in that increasing microbubble technology of preparing, as: coaxial electrostatic atomization, ultrasonic cavitation method, emulsion process, freeze-drying, atomization, high pressure homogenization method, interfacial polymerization and microfiuidic elements method etc.。Microfiuidic elements method namely adopts micro-fluidic chip to prepare microbubble, it it is the new mode of comparison, this mode can be passed through to be accurately controlled liquid with gas thus controlling the structure of bubble, composition and size, owing to the mode of each bubble of preparation is almost consistent, it is ensured that the size of microbubble and polydispersity。
But adopt micro-fluidic chip to prepare in the process of micron dimension bubble, due to the restriction of MCA in existing micro-fluidic chip, experimentation compare very long and loaded down with trivial details by controlling the process of liquid and gas acquisition microbubble, not easily operate, and the microbubble generated is flat and the spherical microbubble of non-cubic。And spherical microbubble is more suitable for the further research and analysis of microbubble flow behavior and movement mechanism。Therefore, for the deficiency of existing micro-fluidic chip internal channel structure。
Summary of the invention
It is an object of the invention to generate the deficiency in microbubble process for existing micro-fluidic chip, it is provided that a kind of new micro-fluidic chip。Microchannel in chip is improved by this micro-fluidic chip, the structure of microchannel is re-started design, changes the size of passage each several part。Thus reach experimentation just can be generated the purpose of microbubble more spherical than existing smaller micron dimension by simple operations。
In order to realize foregoing invention purpose, the technical solution used in the present invention is: a kind of micro-fluidic chip for generating spherical microbubble。Micro-fluidic chip entirety is formed by the chip bonding that MCA in two panels is different, and the bonding face of two panels chip all adopts optical etching technology to etch the microchannel of certain thickness and width。Wherein having two fluid microchannels in the first chip, gas microchannel, microbubble generate passage and microbubble output channel, two fluid microchannels and gas microchannel intersection exist the angle of certain angle；There are the fluid microchannels equivalently-sized with the first chip structure, gas microchannel and microbubble output channel in second chip, but do not have microbubble to generate microchannel。After two panels chip bonding forms, in microchannel, inject liquids and gases simultaneously, by regulating fluid flow, control gas pressure intensity, adopt the liquid of gas with various or different viscosities can generate the microbubble of different-diameter size。
Furtherly, the long 40mm of micro-fluidic chip, wide 20mm, in every chip, two fluid microchannels are equivalently-sized, fluid microchannels wide 30 μm；Gas microchannel is wide 40 μm, and microbubble output channel is wide 100 μm, and fluid microchannels, gas microchannel etching depth are 20 μm, microbubble output channel etching depth 40 μm, and microbubble generates channel width 10 μm, etching depth 10 μm；Microbubble generates the identical width of channel etch and the degree of depth constitutes foursquare, and the angle between fluid microchannels and gas microchannel is 70 °。
The present invention compared with prior art, has the beneficial effect that
1, in existing micro-fluidic chip, the structure of microchannel is all one side etching, and the microbubble of generation is round pie Two-Dimensional Bubble。The present invention proposes micro-fluidic chip and is formed by the first chip and the second chip 2 bonding of different MCAs, in chip, fluid passage, gas passage, microbubble generation passage and the width of microbubble output channel, etching depth are all different, particularly it is etched with microbubble in the first chip and generates passage, wide 10 μm, the degree of depth 10 μm, it is a foursquare cross section that side, sectional microbubble generates passage；Second chip does not etch microbubble and generates passage, and the width degree of depth equidimension of other passages is all identical with chip one。Micro-fluidic chip after interior MCA and size-modify can generate three-dimensional spherical micron dimension microbubble, breaches microbubble that microfiuidic elements the generates limitation in planform。
2, the drawback brought in experimentation for the vertical stratification between existing fluid microchannels and gas microchannel, it is proposed to the angle between fluid microchannels and gas microchannel is changed into the improvement of 70 ° of angles。Microbubble generates in experimentation, liquid phase is flowed out by both sides, gas is produced the effect that folder stream focuses on, gas phase is subject to the symmetrical shear action from both sides liquid phase, broken generation bubble, the MCA after improvement compares original structure, and the generation process of microbubble is more stable, the controlled range generating microbubble size is wider, can generate the diameter microbubble less than gas microchannel size。
3, the present invention is by changing the structure of microchannel in micro-fluidic chip, regulate microchannel each several part different size, adopt this micro-fluidic chip for generating in the experimentation of micron dimension microbubble, operate easier, and the microbubble controlled diameter scope generated is bigger, the microbubble of more minor diameter can be generated, minimum diameter about 20 μm。The microbubble generated is three-dimensional spherical bubble, and spherical microbubble is more suitable for the further research of microbubble movement mechanism and flow behavior。
Accompanying drawing explanation
Fig. 1 is the first chip schematic top plan view in micro-fluidic chip；
Fig. 2 is the second chip schematic top plan view in micro-fluidic chip；
Fig. 3 is micro-fluidic chip side sectional view；
In figure: 1, liquid injection channel, 2 gas injection channels, 3, liquid injection channel, 4 microbubbles generate passages, and 5, microbubble output channel, the 6, first chip, the 7, second chip。
Detailed description of the invention
As Figure 1-3, the micro-fluidic chip being made with PDMS for material is for example, and specific embodiments of the present invention are:
Micro-fluidic chip, cuboid, long 40mm, wide 20mm, high 5mm, it is bonded by the first chip 6 and the second chip 7 and forms, chip surface adopts optical etching technology to etch microchannel, first chip includes liquid injection channel 1, 3, channel width 30 μm, the degree of depth 20 μm, gas injection channel 2, channel width 40 μm, the degree of depth 20 μm, fluid passage 1, 3 with gas passage 2 intersection oblique angle design, angle is 70 °, microbubble generates passage 4, passage length 40 μm, channel width 10 μm, the degree of depth 10 μm, microbubble generates passage side, sectional and may make up the square that the length of side is 10 μm, microbubble output channel 5, channel width 100 μm, the degree of depth 40 μm。Second chip fluid passage, gas passage and microbubble output channel are all identical with the first chip structure size, do not etch the microbubble in the first chip and generate passage 4。By the micro-fluidic chip of the first chip and the second chip bonding, it is square structure that microbubble generates channel cross-section, and the degree of depth that liquid, gas passage generate output channel with microbubble is different, can generate the spherical microbubble of solid。It is simpler quickly that fluid passage and the improvement of gas passage intersection angle make to generate microbubble operation in experimentation。
When adopting the PDMS micro-fluidic chip being made to carry out microbubble generation experiment, owing to PDMS material has elasticity, and the upper and lower two-layer of PDMS micro-fluidic chip is firmly bonded together。Therefore having only in the tack syringe needle insertion chip gently that is equipped with before using, the other end of syringe needle is connected with plastic flexible pipe。Then, by injecting alcohol flushing microchannel in chip under injector for medical purpose low flow velocity, using deionized water rinsing microchannel again, pass into air to microchannel, squeeze out by fluid in chip after a few minutes after rinsing a few minutes, hereafter PDMS chip is in resting state。
In experimentation, the plastic flexible pipe being connected with microchannel 1,3 is connected with injector for medical purpose, the deionized water of 5ml is filled in injector for medical purpose, two injector for medical purpose are installed on precise injection pump, advance syringe to inject liquid with a firm discharge in fluid passage with syringe pump, be installed on another precise injection pump with the plastic flexible pipe being connected of gas passage 2 and injector for medical purpose, in injector for medical purpose, be filled 5ml air, gas flow is set, uses syringe pump propelling gas。Chip other end microbubble output channel is inserted tack syringe needle and is connected in plastic flexible pipe access microbubble reception device。Micro-fluidic chip is positioned under inverted microscope, it can be observed that the process that microbubble generates。Adopt high-speed camera can record not the different shape in microbubble generation process in the same time。Meanwhile, in experiment, it is possible to regulate different fluid floies and gas flow, use the liquid of different viscosities, such as silicone oil, liquid wax etc., or use different gas, such as nitrogen etc., it is possible to control speed that microbubble generates, the microbubble quantity of generation and size。It is smaller that micro-fluidic chip of the present invention relates to, it is possible to produces the spherical microbubble of diameter about 20 μm。
To sum up, microchannel in chip is designed and improves by the present invention, makes experimentation simple to operation, and the microbubble diameter of generation is less and be three-dimensional spherical
The above; it it is only presently preferred embodiments of the present invention; not the present invention is imposed any restrictions, every any simple modification, change and equivalent structure change above example made according to the technology of the present invention essence, all still fall within the protection domain of technical solution of the present invention。

Claims (2)

1. for generating the micro-fluidic chip of spherical microbubble, it is characterised in that: micro-fluidic chip entirety is formed by the chip bonding that MCA in two panels is different, and the bonding face of two panels chip all adopts optical etching technology to etch the microchannel of certain thickness and width；Wherein having two fluid microchannels in the first chip, gas microchannel, microbubble generate passage and microbubble output channel, two fluid microchannels and gas microchannel intersection exist the angle of certain angle；There are the fluid microchannels equivalently-sized with the first chip structure, gas microchannel and microbubble output channel in second chip, but do not have microbubble to generate microchannel；After two panels chip bonding forms, in microchannel, inject liquids and gases simultaneously, by regulating fluid flow, control gas pressure intensity, adopt the liquid of gas with various or different viscosities can generate the microbubble of different-diameter size。
2. the micro-fluidic chip for generating spherical microbubble according to claim 1, it is characterised in that: the long 40mm of micro-fluidic chip, wide 20mm, in every chip, two fluid microchannels are equivalently-sized, fluid microchannels wide 30 μm；Gas microchannel is wide 40 μm, and microbubble output channel is wide 100 μm, and fluid microchannels, gas microchannel etching depth are 20 μm, microbubble output channel etching depth 40 μm, and microbubble generates channel width 10 μm, etching depth 10 μm；Microbubble generates the identical width of channel etch and the degree of depth constitutes foursquare, and the angle between fluid microchannels and gas microchannel is 70 °。
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CN106110340A (en) * 2016-07-12 2016-11-16 陈传品 A kind of method and device preparing target drug-carrying microbubble
CN109030368A (en) * 2018-09-08 2018-12-18 重庆科技学院 Micro-fluidic chip application method associated with a kind of and cuvette
CN109701430A (en) * 2019-01-21 2019-05-03 中国计量大学 A method of vibration pipeline control T-type micro-fluidic chip generates microbubble
CN109833921A (en) * 2017-11-28 2019-06-04 中国科学院大连化学物理研究所 A kind of preparation method of the controllable aqueous two-phase drop of high throughput based on microflow control technique
CN110387313A (en) * 2019-08-07 2019-10-29 哈尔滨工业大学 A kind of magnetophoresis micro-fluidic chip based on bubble mixing
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