CN105688499A - Membrane method extraction refining technology of inosine fermentation liquor - Google Patents
Membrane method extraction refining technology of inosine fermentation liquor Download PDFInfo
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- CN105688499A CN105688499A CN201610148630.2A CN201610148630A CN105688499A CN 105688499 A CN105688499 A CN 105688499A CN 201610148630 A CN201610148630 A CN 201610148630A CN 105688499 A CN105688499 A CN 105688499A
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- inosine
- fermentation broth
- inosine fermentation
- method extraction
- fermentation liquor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D36/00—Filter circuits or combinations of filters with other separating devices
- B01D36/003—Filters in combination with devices for the removal of liquids
- B01D36/006—Purge means
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D36/00—Filter circuits or combinations of filters with other separating devices
- B01D36/02—Combinations of filters of different kinds
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/167—Purine radicals with ribosyl as the saccharide radical
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- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a membrane method extraction refining technology of inosine fermentation liquor. The technology comprises the steps that a, large-particle impurities in inosine fermentation liquor are filtered away through a pipeline filter; b, the inosine fermentation liquor is added into a liquor storage pot to be heated to 60 DEG C for preheating, and pH is adjusted to be 3.5; c, the inosine fermentation liquor is pumped into a ceramic membrane assembly ultrafiltration device for classified concentration and filtration; d, when 30% of flux is left, compressed air in a backflush device jacks in pure water backflushing for pure water top washing; e, the obtained concentrated liquor is subjected to spray drying after entering a concentrated liquor pot to obtain the inosine powder finished product, and clear liquid obtained after filtration is recycled as technology cooling water. The membrane method extraction refining technology of the inosine fermentation liquor is high in filter efficiency, free of pollution and low in production and operation cost.
Description
Technical field
The present invention relates to chemical technology field, particularly relate to the embrane method extraction and purification process of a kind of inosine fermentation broth。
Background technology
Inosine (Inosine) is the nucleic acid product that China's yield is maximum, in medical industry, inosine can be processed into injection, oral liquid or tablet, cell membrane can be directed through and enter human body cell, ATP is produced in cell, making to be in the cellular-restoring normal condition of mental retardation anoxia. the commercial production of inosine is generally adopted microbe fermentation method, then by separating, extract and the refining fine work obtaining inosine from fermentation liquid。Inosine fermentation broth mainly contains inosine, pigment, inorganic salt, residual sugar, thalline and side-product albumen etc.。
Traditional inosine fermentation broth extraction and purification process is to complete through three steps of lowering the temperature, be centrifuged, wash, temperature-fall period is, in the short time, the temperature of product is dropped to less than 5 DEG C, make its fractional crystallization then through centrifuge, be then centrifuged again through pure water, finally obtain just inosine product。First inosine is re-fed into refining plant and refines further, bleacher decolours, mainly by the pigment of activated carbon adsorption product surface and impurity in bleacher, again through the air pressure filter below bleacher by activated carbon elimination, after these techniques, the product of output is exactly inosine。
Owing to treating capacity is relatively big, the temperature-fall period in traditional handicraft needs to consume substantial amounts of energy；Activated carbon decolorizing process cannot thoroughly be removed due to activated carbon, can affect the purity of final products, and activated carbon also cannot reclaim；Washing is relatively big by pure water amount, and owing to can contain a certain amount of just inosine product in water after washing, direct discharge can cause environmental pollution, will also result in the loss of product, and the inosine yield of traditional handicraft is approximately in about 90%。
Summary of the invention
The present invention is directed to the above technical problem existed in prior art, it is proposed to the embrane method extraction and purification process of a kind of inosine fermentation broth, filter efficiency is high, pollution-free and production run cost is low。
The technical solution adopted for the present invention to solve the technical problems is: the embrane method extraction and purification process of a kind of inosine fermentation broth, and it includes step:
A. inosine fermentation broth filters large granular impurity through piping filter；
B. enter fluid reservoir to be warmed to 60 DEG C and carry out preheating and prepare, and adjust pH to 3.5；
C. it is pumped to ceramic film component ultrafiltration apparatus and carries out multi-stage PCE filtration, ceramic film component ultrafiltration apparatus adopts two-stage series connection, the first order adopts the tubular ceramic membrane module of three 50nm specifications in parallel, and the second level adopts the tubular ceramic membrane module of three 20nm specifications in parallel, and two-stage is internal recycle；
D. when flux surplus 30% time by the device that recoils in compression air head into pure water recoil and carry out pure water top and wash, for the pollution of phase shift films, the flux remained stable for, the dope finally discharged concentrates three times, and dope amount is controlled in the 25% of stock solution by dense hydraulic control valve；
E. gained dope obtains inosine powder finished product through spray drying after entering dope tank, and after filter, clear liquid recycles as process cooling water。
As a kind of improvement to technical scheme of the present invention, in described step a, inosine fermentation broth reaches the tank volume late gate of 70% in storage tank and automatically turns on, sensor carry out the volume of inosine fermentation broth in storage tank and be monitored and autocontrol valve unlatching。
As a kind of improvement to technical scheme of the present invention, in described step b, operation pressure is 0.8MPa, and cycles of concentration is 3 times, crossflow velocity 3.8m/s, average flux 40L/m2/ h, operation temperature is 70 DEG C, and membrane operations pressure reduction is 0.2MPa。
As a kind of improvement to technical scheme of the present invention, circulating pump provide the pressure loss in flow velocity and flow process；Required inosine fermentation broth and pressure are provided by a frequency conversion charging pump system；Two-stage penetrating fluid flow is by governor valve control。
As a kind of improvement to technical scheme of the present invention, one batch of production carries out ceramic film component ultrafiltration apparatus cleaning after terminating, after cleaning film 40min with mass fraction for 1%NaOH and 0.2%NaClO mixed solution, then cleaning 5min with the HNO3 solution of 0.5%, membrane flux can recover rapidly。
The embrane method extraction and purification process of inosine fermentation broth provided by the invention, filter efficiency is high, pollution-free and production run cost is low。
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearly understand, below in conjunction with specific embodiment, the present invention is further elaborated。Should be appreciated that specific embodiment described herein is only in order to explain the present invention, is not intended to limit the present invention。
The embrane method extraction and purification process of the inosine fermentation broth of the specific embodiment of the invention, it includes step:
A. inosine fermentation broth filters large granular impurity through piping filter；
B. enter fluid reservoir to be warmed to 60 DEG C and carry out preheating and prepare, and adjust pH to 3.5；
C. it is pumped to ceramic film component ultrafiltration apparatus and carries out multi-stage PCE filtration, ceramic film component ultrafiltration apparatus adopts two-stage series connection, the first order adopts the tubular ceramic membrane module of three 50nm specifications in parallel, and the second level adopts the tubular ceramic membrane module of three 20nm specifications in parallel, and two-stage is internal recycle；
D. when flux surplus 30% time by the device that recoils in compression air head into pure water recoil and carry out pure water top and wash, for the pollution of phase shift films, the flux remained stable for, the dope finally discharged concentrates three times, and dope amount is controlled in the 25% of stock solution by dense hydraulic control valve；
E. gained dope obtains inosine powder finished product through spray drying after entering dope tank, and after filter, clear liquid recycles as process cooling water。
In the specific embodiment of the invention, in described step a, inosine fermentation broth reaches the tank volume late gate of 70% in storage tank and automatically turns on, sensor carry out the volume of inosine fermentation broth in storage tank and be monitored and autocontrol valve unlatching。In described step b, operation pressure is 0.8MPa, and cycles of concentration is 3 times, crossflow velocity 3.8m/s, average flux 40L/m2/ h, operation temperature is 70 DEG C, and membrane operations pressure reduction is 0.2MPa。The pressure loss in flow velocity and flow process is provided by circulating pump；Required inosine fermentation broth and pressure are provided by a frequency conversion charging pump system；Two-stage penetrating fluid flow is by governor valve control。
Preferably, a batch of production carries out ceramic film component ultrafiltration apparatus cleaning after terminating, and after cleaning film 40min with mass fraction for 1%NaOH and 0.2%NaClO mixed solution, then cleans 5min with the HNO3 solution of 0.5%, and membrane flux can recover rapidly。
The present invention adopts the tubular ceramic membrane of two specifications of 20nm and 50nm for extracting the ceramic membrane aperture filtering inosine fermentation broth, and every membrane tube has 19, passage。This device adopts two-stage series connection mode, and every grade all adopts three parallel connections, and total of six membrane module connects to form。
Inosine fermentation broth filters large granular impurity through piping filter, it is warmed to 60 DEG C subsequently into fluid reservoir and carries out preheating preparation, and adjust pH to 3.5, two grades that are pumped to that membrane aperture is 50nm/20um and 6 membrane module monomers of allying the communists carry out multi-stage PCE filtration, operation pressure is 0.8MPa, cycles of concentration is 3 times, crossflow velocity 3.8m/s, average flux 40L/m2/h, operation temperature can obtain higher face permeation flux when being about 70 DEG C, membrane operations pressure reduction is 0.2MPa, pure water recoil is headed into by compression air in the device that recoils when flux remains 30%, pollution for phase shift films, the flux remained stable for, the concentration finally discharging dope is controlled in about the 25% of stock solution by dense hydraulic control valve, gained dope obtains inosine powder finished product through spray drying after entering dope tank, high selectivity due to ceramic membrane aperture, the rejection of effective ingredient reaches more than 99.6%, after filter, clear liquid clear can recycle as process cooling water。
Dope is partially separated the process that concentration can refilter through three washing dilutions, as much as possible to isolate effective ingredient, because all active substance all cannot pass through high selective atom level filters fenestra, so the rejection of theory can reach 100%, after making filter, clear liquid impurities is preferably minimized simultaneously, gained clear liquid can recycle as process cooling water, and environment will not cause any pollution。
Advantages of the present invention and effect be: 1, due to the high selectivity of this art ceramics membrane aperture, the rejection of effective ingredient can reach more than 99.6%；Production process does not use centrifugal separation equipment, decreases equipment investment；Without adsorbents such as activated carbons in subtractive process, improve product quality and yield concentration；Leach clear liquid to recycle as cooling water, environment will not be caused any pollution；2, inorganic ceramic film has the advantages such as high temperature resistant, acid-alkali-corrosive-resisting, pore-size distribution are narrow, mechanical strength is high, the good and resistance to microbial attack of chemical stability, renewable long service life；3, this process device structure is simple, floor space is little, energy consumption level is low, can close continuous and automatic and run；
It should be appreciated that for those of ordinary skills, it is possible to improved according to the above description or converted, and all these are improved and convert the protection domain that all should belong to claims of the present invention。
Claims (5)
1. the embrane method extraction and purification process of an inosine fermentation broth, it is characterised in that include step:
A. inosine fermentation broth filters large granular impurity through piping filter；
B. enter fluid reservoir to be warmed to 60 DEG C and carry out preheating and prepare, and adjust pH to 3.5；
C. it is pumped to ceramic film component ultrafiltration apparatus and carries out multi-stage PCE filtration, ceramic film component ultrafiltration apparatus adopts two-stage series connection, the first order adopts the tubular ceramic membrane module of three 50nm specifications in parallel, and the second level adopts the tubular ceramic membrane module of three 20nm specifications in parallel, and two-stage is internal recycle；
D. when flux surplus 30% time by the device that recoils in compression air head into pure water recoil and carry out pure water top and wash, for the pollution of phase shift films, the flux remained stable for, the dope finally discharged concentrates three times, and dope amount is controlled in the 25% of stock solution by dense hydraulic control valve；
E. gained dope obtains inosine powder finished product through spray drying after entering dope tank, and after filter, clear liquid recycles as process cooling water。
2. the embrane method extraction and purification process of inosine fermentation broth according to claim 1, it is characterized in that, in described step a, inosine fermentation broth reaches the tank volume late gate of 70% in storage tank and automatically turns on, sensor carry out the volume of inosine fermentation broth in storage tank and be monitored and autocontrol valve unlatching。
3. the embrane method extraction and purification process of inosine fermentation broth according to claim 1, it is characterised in that in described step b, operation pressure is 0.8MPa, and cycles of concentration is 3 times, crossflow velocity 3.8m/s, average flux 40L/m2/ h, operation temperature is 70 DEG C, and membrane operations pressure reduction is 0.2MPa。
4. the embrane method extraction and purification process of inosine fermentation broth according to claim 1, it is characterised in that the pressure loss in flow velocity and flow process is provided by circulating pump；Required inosine fermentation broth and pressure are provided by a frequency conversion charging pump system；Two-stage penetrating fluid flow is by governor valve control。
5. the embrane method extraction and purification process of inosine fermentation broth according to claim 1, it is characterized in that, one batch of production carries out ceramic film component ultrafiltration apparatus cleaning after terminating, after cleaning film 40min with mass fraction for 1%NaOH and 0.2%NaClO mixed solution, cleaning 5min with the HNO3 solution of 0.5% again, membrane flux can recover rapidly。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610148630.2A CN105688499A (en) | 2016-03-16 | 2016-03-16 | Membrane method extraction refining technology of inosine fermentation liquor |
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| CN201610148630.2A CN105688499A (en) | 2016-03-16 | 2016-03-16 | Membrane method extraction refining technology of inosine fermentation liquor |
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| CN105688499A true CN105688499A (en) | 2016-06-22 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113185563A (en) * | 2021-04-26 | 2021-07-30 | 济南明鑫制药股份有限公司 | Method for purifying inosine from fermentation liquor |
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2016
- 2016-03-16 CN CN201610148630.2A patent/CN105688499A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113185563A (en) * | 2021-04-26 | 2021-07-30 | 济南明鑫制药股份有限公司 | Method for purifying inosine from fermentation liquor |
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Application publication date: 20160622 |
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