CN1056852C - Method for extracting water-soluble spirulina polyose - Google Patents
Method for extracting water-soluble spirulina polyose Download PDFInfo
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- CN1056852C CN1056852C CN94105389A CN94105389A CN1056852C CN 1056852 C CN1056852 C CN 1056852C CN 94105389 A CN94105389 A CN 94105389A CN 94105389 A CN94105389 A CN 94105389A CN 1056852 C CN1056852 C CN 1056852C
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- spirulina
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- polyose
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Abstract
The present invention relates to a method for extracting water soluble spirulina polysaccharide, which comprises the steps of extracting spirulina by alkaline solution, utilizing organic acid for neutralization and precipitating water soluble polysaccharide by an organic solvent. The method can fully utilize spirulina resources, and a reagent with no toxicity nor volatility is adopted, so that the environment is not polluted. The yield is as high as 1.9%, and the method also has the advantages of convenient operation and industrialized production.
Description
The present invention relates to extract from algae the method for water-soluble polysaccharide, this water-soluble polysaccharide can be used for making medicine, healthcare products, food etc.
Spirulina is found in the African Chad lake the earliest, is now carried out artificial culture by people widely.Find through years of researches, spirulina has very strong radiation resistance, its radiation-resistant biologically active substance is a kind of water-soluble polysaccharide of being made up of D-glucose, D-seminose, D-semi-lactosi, four kinds of monose of D-glucuronic acid, this water-soluble polysaccharide also has the effect of enhance immunity function and to the restraining effect of transplantability cancer cells except radiation resistance is arranged.Roll up the 5th phase 445 pages of methods of purification that disclose the spirulina antiradiation polysaccharide " Acta Biochimica et Biophysica Sinica " September the 21st in 1989, promptly from spirulina, extract polysaccharide with warm water, filter then, remove the gred, concentrate, remove Deproteinization with chloroform-propyl carbinol method, go out polysaccharide with ethanol sedimentation, yield is a thousandth, and there is following shortcoming in this method:
1, extremely bothers with the warm water extraction polysaccharide, only be applicable to laboratory scale to be unfavorable for suitability for industrialized production;
2, yield is low, can not make full use of resource;
3, the natural blue pigment material in the spirulina is destroyed, causes the polysaccharide protein activity destroyed;
4, chloroform-propyl carbinol costs an arm and a leg, and because chloroform is poisonous, therefore highly volatile, contaminate environment are removed albumen with chloroform-propyl carbinol method and be restricted again.
The objective of the invention is to above-mentioned defective, a kind of extracting method of water-soluble spirulina polyose is provided, adopt nontoxic reagent at prior art, can significantly improve the yield of polysaccharide, not destroy the natural blue pigment material, free from environmental pollution, easy to operate, thus help suitability for industrialized production.
Method provided by the invention is with alkaline solution extracting spirulina, with the organic acid neutralization, goes out water-soluble polysaccharide with organic solvent deposit.
Alkaline solution of the present invention is that consumption is 5～10 parts of weight of spirulina powder, concentration is 2～5% potassium hydroxide, sodium hydroxide, sodium hydrogen carbonate solution or its mixture, the extraction temperature of spirulina powder in alkaline solution is 40～80 ℃, and soaking time is 4～8 hours; The organic acid that the present invention adopts comprises formic acid, acetate, propionic acid, citric acid solution or its mixture, and this organic acid is regulated pH value to 5～6 of said mixture; Organic solvent is ethanol, acetone, ethyl acetate solution or its mixture, and its consumption is 2～4 times of concentrated solution volume.
With in the organic acid and after mixture need leave standstill throw out is precipitated fully to take out clear liquid, this clear liquid is concentrated into original ～ volume, uses organic solvent deposit then, dry sediment gets water-soluble polysaccharide.Above-mentioned organic acid and organic solvent can directly use commercially available industrial reagent.
The natural blue pigment material is dissolved in the organic solvent, and it is reusable to reclaim organic solvent, and simultaneously recyclable natural blue pigment material imitating element is used.With in the organic acid and after drying precipitate after can be used as the high-grade feed additive and use.
The present invention compared with prior art has following advantage:
1, make full use of the spirulina resource, with in the organic acid and after throw out can be used as fodder additives, be dissolved in the recyclable imitating element usefulness of natural blue pigment material in the organic solvent, the also recyclable repeated use of organic solvent;
2, use nontoxic, non-volatility reagent, free from environmental pollution;
3, the yield height reaches 1.9%;
4, easy to operate, but suitability for industrialized production.
Be described in further detail method of the present invention below by embodiment.
Embodiment 1
Getting dry spirulina powder 100 grams washes with water 3 times, add concentration and be 800 milliliters of the sodium hydroxide solutions of 2.5% weight, be incubated 8 hours down at 40 ℃, adding industrial acetate then, to be neutralized to the solution pH value be 5, left standstill 24 hours, the taking-up clear liquid is concentrated into times of original volume, adds the industrial ethyl alcohol that is equivalent to 2 times of volumes of concentrated solution and precipitates, dry sediment gets 1.9 gram water-soluble polysaccharides, so yield is 1.9%.
Be dissolved in the recyclable imitating element usefulness of natural blue pigment material in the ethanolic soln, the also recyclable repeated use of ethanol.
Add in the acetic acid solution and after drying precipitate after can be used as the additive of high-grade feed.
Embodiment 2
Getting 10 kilograms of dry spirulina powders washes with water 2 times, add concentration and be 50 liters of the sodium hydrogen carbonate solutions of 5% weight, be incubated 7 hours down at 50 ℃, being neutralized to pH value with industrial formic acid then is 6, left standstill 24 hours, the taking-up clear liquid is concentrated into times of original volume, adds the industrial acetone that is equivalent to 4 times of volumes of concentrated solution and precipitates, dry sediment gets 0.18 kg of water soluble polysaccharide, so yield is 1.8%.
Embodiment 3
Getting 20 kilograms of dry spirulina powders washes with water 2 times, add concentration and be 200 liters of the potassium hydroxide solutions of 3% weight, be incubated 5 hours down at 70 ℃, be that to be neutralized to pH value be 6 for the citric acid solution of 10% weight with concentration then, left standstill 24 hours, the taking-up clear liquid is concentrated into times of original volume, adds the industrial ethyl acetate solution that is equivalent to 3 times of volumes of concentrated solution and precipitates, dry sediment gets 0.32 kg of water soluble polysaccharide, so yield is 1.6%.
Embodiment 4
Getting 50 kilograms of dry spirulina powders washes with water 3 times, adding concentration is 300 liters of 2% weight sodium hydroxide solutions, be incubated 4 hours down at 80 ℃, be neutralized to pH value 5.5 with industrial propionic acid then, left standstill 24 hours, the taking-up clear liquid is concentrated into times of original volume, adds the industrial alcohol that is equivalent to concentrated 3 times of volumes and precipitates, dry sediment gets 0.75 kg of water soluble polysaccharide, so polysaccharide yield is 1.5%.
Claims (3)
1, a kind of extracting method of water-soluble spirulina polyose is characterized in that with the organic acid neutralization, going out water-soluble polysaccharide with organic solvent deposit with alkaline solution extracting spirulina; Used alkaline solution is that consumption is 5～10 parts of weight of spirulina powder, and concentration is 2～5% potassium hydroxide, sodium hydroxide, sodium hydrogen carbonate solution or its mixture, and the extraction temperature of spirulina powder in alkaline solution is 40～80 ℃, and soaking time is 4～8 hours; Used organic acid comprises formic acid, acetate, propionic acid, citric acid solution or its mixture, and this organic acid is regulated pH value to 5～6 of said mixture.
2, according to the extracting method of the described water-soluble spirulina polyose of claim 1, it is characterized in that organic solvent is ethanol, acetone, ethyl acetate solution or its mixture, its consumption is 2～4 times of concentrated solution volume.
3, according to the extracting method of the described water-soluble spirulina polyose of claim 1, it is characterized in that with in the organic acid and after mixture leave standstill throw out precipitated fully, take out the stillness of night then to be concentrated into 1/4～1/2 times of original volume.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94105389A CN1056852C (en) | 1994-05-16 | 1994-05-16 | Method for extracting water-soluble spirulina polyose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94105389A CN1056852C (en) | 1994-05-16 | 1994-05-16 | Method for extracting water-soluble spirulina polyose |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1112128A CN1112128A (en) | 1995-11-22 |
| CN1056852C true CN1056852C (en) | 2000-09-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN94105389A Expired - Fee Related CN1056852C (en) | 1994-05-16 | 1994-05-16 | Method for extracting water-soluble spirulina polyose |
Country Status (1)
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067404C (en) * | 1997-05-26 | 2001-06-20 | 汕头经济特区鮀滨化学药业总公司 | Method for extraction water soluble polyose of spirulina |
| CN103694370B (en) * | 2013-12-31 | 2015-10-28 | 福建省神六保健食品有限公司 | A kind of preparation method of spirulina polysaccharide |
| US10070506B2 (en) * | 2014-04-07 | 2018-09-04 | Lumileds Llc | Ignitor-arrangement |
| CN105837703A (en) * | 2016-05-25 | 2016-08-10 | 丽江格林斯通食品有限公司 | Method for extracting spirulina polysaccharide |
| CN111732671A (en) * | 2020-07-17 | 2020-10-02 | 王志忠 | Preparation method of spirulina polysaccharide |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2131802A (en) * | 1982-12-08 | 1984-06-27 | Procter & Gamble | Solvent decolourisation of alkyl saccharides |
| JPS6131095A (en) * | 1984-07-24 | 1986-02-13 | Kazutaka Shinohara | Mucilaginous polysaccharide and preparation thereof |
| JPS61167620A (en) * | 1985-01-19 | 1986-07-29 | Toa Nenryo Kogyo Kk | Carcinostatic agent containing polysaccharide |
| EP0514890A2 (en) * | 1991-05-22 | 1992-11-25 | Shin-Etsu Chemical Co., Ltd. | Method for purifying polysaccharides |
-
1994
- 1994-05-16 CN CN94105389A patent/CN1056852C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2131802A (en) * | 1982-12-08 | 1984-06-27 | Procter & Gamble | Solvent decolourisation of alkyl saccharides |
| JPS6131095A (en) * | 1984-07-24 | 1986-02-13 | Kazutaka Shinohara | Mucilaginous polysaccharide and preparation thereof |
| JPS61167620A (en) * | 1985-01-19 | 1986-07-29 | Toa Nenryo Kogyo Kk | Carcinostatic agent containing polysaccharide |
| EP0514890A2 (en) * | 1991-05-22 | 1992-11-25 | Shin-Etsu Chemical Co., Ltd. | Method for purifying polysaccharides |
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| Publication number | Publication date |
|---|---|
| CN1112128A (en) | 1995-11-22 |
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