CN105684967A - Fish bold and shy behavior assessment method - Google Patents
Fish bold and shy behavior assessment method Download PDFInfo
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- CN105684967A CN105684967A CN201610054518.2A CN201610054518A CN105684967A CN 105684967 A CN105684967 A CN 105684967A CN 201610054518 A CN201610054518 A CN 201610054518A CN 105684967 A CN105684967 A CN 105684967A
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- 238000000034 method Methods 0.000 title claims abstract description 28
- 241000251468 Actinopterygii Species 0.000 claims abstract description 70
- 230000003578 releasing effect Effects 0.000 claims abstract description 24
- 238000002474 experimental method Methods 0.000 claims abstract description 17
- 235000013305 food Nutrition 0.000 claims abstract description 15
- 238000002372 labelling Methods 0.000 claims abstract description 12
- 230000009747 swallowing Effects 0.000 claims abstract description 5
- 239000011521 glass Substances 0.000 claims description 30
- 244000062645 predators Species 0.000 claims description 13
- 238000003556 assay Methods 0.000 claims description 10
- 230000003031 feeding effect Effects 0.000 claims description 9
- 230000003203 everyday effect Effects 0.000 claims description 8
- 239000004512 granular bait Substances 0.000 claims description 6
- 238000004458 analytical method Methods 0.000 claims description 4
- 235000012631 food intake Nutrition 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 238000004445 quantitative analysis Methods 0.000 claims description 4
- 241001439627 Schistostoma Species 0.000 claims description 3
- 241000122938 Strongylus vulgaris Species 0.000 claims description 3
- 230000006978 adaptation Effects 0.000 claims description 3
- 239000011324 bead Substances 0.000 claims description 3
- 230000003542 behavioural effect Effects 0.000 claims description 3
- 238000009395 breeding Methods 0.000 claims description 3
- 230000001488 breeding effect Effects 0.000 claims description 3
- 230000007613 environmental effect Effects 0.000 claims description 3
- 230000013011 mating Effects 0.000 claims description 3
- 238000002560 therapeutic procedure Methods 0.000 claims description 3
- 239000003086 colorant Substances 0.000 claims description 2
- 238000011084 recovery Methods 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 2
- 230000006399 behavior Effects 0.000 abstract 6
- 230000003044 adaptive effect Effects 0.000 abstract 1
- 230000037406 food intake Effects 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 238000012360 testing method Methods 0.000 description 6
- 238000009792 diffusion process Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical group [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- 241000264847 Siniperca chuatsi Species 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 239000008239 natural water Substances 0.000 description 2
- 238000004080 punching Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000013139 quantization Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
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- 230000001228 trophic effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a fish bold and shy behavior assessment method and relates to a method for obtaining fish personality characteristic behavior data. The assessment method includes the following steps that firstly, experiment fishes are selected and bred; secondly, maximum intake and swallowing time of individuals are determined; thirdly, paired labeling, adaptive restoration and setting of food abundance and predation risks are conducted; fourthly, behavior data is obtained; fifthly, behaviors are quantitatively analyzed and calculated. The assessment method is applied to artificial releasing of fishes. Bold fishes are selected, and a releasing place which is high in food abundance and low in predation risk is selected. The assessment method is simple and interference is eliminated. The assessment method is standard and unified, risky ingestion behaviors of the fishes are precisely quantified, and persuasive and quantitative risk behavior precise parameters are provided for comprehensive assessment of bold and shy personality characteristics. Making of raised fish artificial releasing technology rules and assessment of releasing effect of the rules are facilitated, releasing efficiency is improved, and influences of artificial releasing on local fishes are reduced.
Description
Technical field
The present invention relates to a kind of method obtaining Fish personal characteristics behavioral data, particularly relate to the assay method of a kind of Fish courageously shy behavior。
Background technology
Fish have mobile trend and explore the performance of strange things (resource and space) and be courageously, and it is the personal characteristics that animal is important, a major incentive for individual diffusivity variation in being kind。The personal characteristics of Fish " bold-shy " not only influences whether whether they spread or settle down, it is also possible to can affect the diffusion length of Fish。Therefore, more bold Fish are likely to also can be better diffusion person。
The research of fitness before artificial domestication fish-releasing is not only the focus of current international research, and for raising and train the specification of releasing of Fish, quantity of releasing, rationally the selecting and determine offer scientific basis of release season and place etc. of releasing。Fish are courageously or shyly important personal characteristics closely-related with fitness, obtain the Fish the most direct environment of personal characteristics and come from natural water, but because natural water habitat is complicated and changeable, observe difficulty, therefore should not design related experiment in the wild accurately to measure, rely only on lab simulation natural environment and take proper method indirectly to measure。More existing bold personal characteristics utilizing the interior individual risk of straightforward procedure mensuration population to enter strange habitat, but there is obvious shortcoming in its mensuration:
1. compellent quantizating index parameter is lacked；
2. do not take effective anti-interference method to ensure the reliability of data；
3. unified mensuration specification is lacked。
Accurately measuring of Fish " bold-shy " personal characteristics is all created adverse effect by disadvantage mentioned above, it is difficult to accurate evaluation Fish diffusion potentiality, the determination that the Fish specification when artificial releasing is chosen, released place and season of releasing is raised and train in interference, affects the foundation of artificial releasing's effect evaluation system。Therefore, unified and standard quantitative determination method is needed badly for the Fish of different building shape, different size。By grasping Fish " bold-shy " personal characteristics, predict and determine that it raises and train the diffusion potentiality of population, be conducive to formulating scientific and effective, unified and standard raise and train Technique in Fishes liberation techniques code, and artificial releasing's effect offer scientific basis of Fish is provided for assessment, it is favorably improved efficiency of releasing, reduces artificial releasing's impact on indigenous strains。
Summary of the invention
It is an object of the invention to for raising and train the fitness evaluation before Technique in Fishes is released, accurately measure the risk taking behavior parameter of Fish, develop the assay method of a kind of Fish courageously shy behavior。
One, the assay method (abbreviation method) of Fish courageously shy behavior
This method comprises the following steps:
1. selecting and raising of fish is tested
A, raising and train fish parent in pond culture, wild fish parent of the same race is trapped in field, and the two all cultivates in pond with reference to the cultivation density raising and train fish；
B, mating period synchronize breeding, and opening fry is separately raised in two ponds that environmental condition is identical by different lines；
C, after 30 days, choose the similar type of raising and train of size and wild type and separately support temporarily in two indoor culture cylinders, being uniformly the satiated with food every day frost blood worm twice of throwing something and feeding；
D, maintain this throw something and feed mode until experiment fish schistostoma be suitable for sunk type granular bait for fish of ingesting；
2. individual maximum food consumption and the determination swallowing the time
A, choose 10 similar tails of size and raise and train fish and 10 tail wild fishes, separately raise in 10 glass jars；
B, Therapy lasted 3 days, throw something and feed 2 times, when reaching fed state every day, it is determined that raise and train fish and wild fish is on average ingested the maximum of sunk type granular bait for fish and swallow the time every time；
3. match labelling and adapt to recovery and food abundance and predation risk setting
A, formal first 5 days measured, measure body length to the fish of two strains and weigh；
B, pick out size similar raise and train fish and wild fish forms matched group at random, two kinds of fish quantity of each matched group are equal；
C, labelling and adaptation recover
With the different types of fish in the small plastic of the different colours beads with holes same cylinder of random labelling；
The pairing fish of labelling is transferred in the relief area of determinator and adapt to；
D, food abundance and predation risk set
Determinator is respectively provided with two kinds of experimental conditions:
A, high abundance without Predator or low abundance without Predator；
B, high abundance have Predator or low abundance without Predator；
4. the acquisition of behavioral data
Throwing something and feeding with photographic head record and raise and train the feeding activity of fish and wild fish in process, data are recorded in real time in the hard disk of computer through video frequency collection card, in order to follow-up video recording analysis；
5. behavior quantitative analysis and calculating:
Computer is transferred Video Document, observe and add up following parameter: in 20 minutes of bait throwing in, every 30 seconds as a timing node, record the two kinds of fishes of each timing node individual amount of ingesting in different bait throwing in districts, calculate same gene type fish and participate in the ratio P of feeding activityf=F/4, wherein F is the quantity participating in ingesting。
The present invention has following advantages and good effect:
1. assay method is simple, disturb elimination；
2. assay method is unified and standard, the precise quantification risk trophic behavior of Fish, provides the risk taking behavior accurate parameters of the quantization of conviction power for the personal characteristics of synthesis measuring " courageously-shy "；
3. be conducive to raising and train the evaluation of the formulation of Technique in Fishes liberation techniques code and effect of releasing thereof, improve efficiency of releasing, reduce artificial releasing's impact on indigenous strains。
Accompanying drawing explanation
Fig. 1 is the structural representation of former determinator；
Fig. 2 .1 is the structural representation (without predation risk) of this determinator；
Fig. 2 .2 is the structural representation (having predation risk) of this determinator。
Fig. 2 .1, in 2.2:
0 glass test cylinder；
BS Predator；
The CS person of participating in the experiment；
D place of safety；
F1The calm danger zone of high abundance；
F2High abundance high risk area,
E relief area；
The left and right funnel of throwing something and feeding of A1, A2；
The left and right glass culture dish of B1, B2。
Detailed description of the invention
Describe in detail below in conjunction with drawings and Examples:
One, determinator
This determinator is to improve on the basis of former determinator (CN204047626U)。
Such as Fig. 1, former determinator includes cross bar 1, hook 2, fluorescent lamp 3, glass jar 4, copper ring 5, black plastic plate 6, punching glass plate 7, uncovered glass culture dish 8, funnel 9, photographic head 10, data line 11, display 12, display transmission line 13 and host computer 14；
Rectangular glass jar 4 is divided into trisection left, center, right glass jar 4.1,4.2,4.3 by two pieces of punching glass plates 7；
Left and right sides at glass jar 4 are respectively arranged with fluorescent lamp 3；
Being provided above with cross bar 1 at glass jar 4, be respectively arranged with copper ring 5 and funnel 9 thereof at left and right two place of the mid-point of cross bar 1, the lower outlet alignment of two funnels 9 is arranged on the upper inlet of two uncovered glass culture dishs 8 bottom middle glass jar 4.2 both sides；
Mid-point at cross bar 1 is connected to hook 2, and the photographic head 10 being suspended on hook 2 is directed at the water that glass jar 4 contains；
With 4 blocks of black plastic plates 6, glass jar surrounding is wrapped up, with black plastic plate 6, glass jar top is sealed；
Photographic head 10, data line 11, host computer 14, display transmission line 13 and display 12 are sequentially connected with。
As Fig. 2 .1,2.2, this determinator has a rectangle glass test cylinder (jar) 0, long × wide × high: 1.75 × 0.35 × 0.35m, it is provided with groove gap in the both sides, inside of glass test cylinder 0, with 4 transparent 1,2,3,4 glass baffle plate C1, C2, C3, C4, glass test cylinder 0 is divided into 5 compartments, both end of which is the long big compartment for 0.5m, intends putting Predator BS；All the other 3 equal-sized boothes, long is 0.25m, intends putting the person of participating in the experiment CS；The left booth being close to big compartment is considered as place of safety D, and the right booth being close to big compartment is considered as the calm danger zone F of high abundance1Or high abundance high risk area F2, middle booth is considered as relief area E；
A1, A2 are left and right funnel of throwing something and feeding (food source)；
B1, B2 are left and right glass culture dish；
Ratio B1:B2=1:2(120:240 sunk type bait of food abundance)。
Two, assay method
For Oncorhynchi, implement specifically comprising the following steps that of the present invention
1. the source of fish is tested
A, the Oncorhynchi parent raised and train are in pond culture, and wild Oncorhynchi parent of the same race is trapped in field, and the two all cultivates in pond with reference to the cultivation density raising and train Oncorhynchi；
B, mating period synchronize breeding, and the opening fry of Oncorhynchi is separately raised in two ponds that environmental condition is identical by different lines, if desired through Molecular Identification to confirm the difference of its strain；
C, after 30 days, choose the similar type of raising and train of size and wild type some (individual weight is about 10g), separately support temporarily in two indoor culture cylinders by the difference of strain, being uniformly the satiated with food every day frost blood worm twice of throwing something and feeding；
D, maintain this throw something and feed mode until experiment fish schistostoma be suitable for sunk type granular bait for fish of ingesting, diameter: 1.54mm, length: 2.89mm, weight: 5.52mg；Protein: 36.0%, fat: 12.1%, energy: 17.8KJg-1, moisture: 10.7%；Water temperature during this period controls at 25.0 ° of C。
2. individual maximum food consumption and the determination swallowing the time
A, choose 10 similar tails of size and raise and train Oncorhynchi and the wild Oncorhynchi of 10 tails, separately raise in 10 glass jars (long × wide × high: 0.2 × 0.2 × 0.2m, the depth of water: 0.15m), it is determined that maximum food consumption that two strains are individual and swallow the time；
B, Therapy lasted 3 days, throw something and feed 2 times, when reaching fed state every day, it is determined that raising and train Oncorhynchi bait of on average every time ingesting is 35.3, and wild Oncorhynchi on average ingests every time, bait is 21.0；
In C, experiment, because not having any tail fish manipulation to process the time of (swallowing) single bait more than 30 seconds, therefore, when intercepting video clip and analyzing, adopt 30 seconds timing nodes as counting；
D, in whole experimentation, experimental water through filtration, precipitation and uv disinfection process, during experiment, water temperature controls at 25 ° of C, dissolved oxygen 8.5mgl-1, pH value 7.7。
3. the setting of assay device and the labelling of experiment fish process and adapt to recover
A, formal first 5 days measured, measure body length (being accurate to 0.01cm) weigh (being accurate to 0.01g) to the Oncorhynchi of two strains；
B, pick out 48 similar tails of size and raise and train Oncorhynchi and the wild Oncorhynchi of 48 tails forms 12 matched groups (average body long 6.62cm, average weight 3.41g), each 4 tails of two kinds of Fish of each matched group at random；
C, mensuration
Determinator 12 is set, as Fig. 2 .1,2.2；
Before a, mensuration start, place 4 tails in the relief area E in every 1 glass jar and raise and train Oncorhynchi and 4
The wild Oncorhynchi of tail；In order to enable substantially to distinguish the identity of two strain fishes, with the small plastic of black and white beads with holes (diameter: 2.3 ± 0.2mm；Weight: 14.2 ± 0.8mg) different types of Oncorhynchi in the same cylinder of random labelling；The pairing fish of labelling is transferred in the relief area E of 16 said determination devices and adapt to；Each glass jar has two fluorescent lamps (8W) in order to the simulating nature photoperiod from 15cm place above the water surface, glass jar surrounding all has black plastic plate to block, make light homogeneous, carry out, with videocorder, the shooting record that takes a crane shot during experiment, the surrounding black plastic cloth of whole determinator seals, in case artificial disturbance；Depth of water 0.2m, bottom is paved with the white corallite that about 2cm is thick, it is the glass culture dish white, that diameter the is 12cm district of ingesting as experiment fish using two bottoms, it is respectively embedded in the bottom corallite of two place of safety/risk area, it is close to big compartment, in case the granular bait for fish not being ingested spreads because of the stirring of Fish, it is also convenient for the clear view experiment fish picked-up situation to food；Being fixed with left and right funnel A1, A2 of throwing something and feeding above it for as bait thrower, each institute bait throwing in material all can fall in left and right glass culture dish B1, B2 exactly；During processing adaptation (the 2nd to the 4th day), carry out 2 times throw something and feed (8:00h-11:00h and 14:00h-17:00h) every day, mode of throwing something and feeding is as follows: before throwing something and feeding, and first 2 piece the 2nd in relief area E two end slot gap, 3 glass baffle plate C2, C3 is slowly removed, throws something and feeds after 3 minutes；When throwing something and feeding, throw something and feed for two and a little throw something and feed simultaneously, but the bait that one end that bait abundance is high (high abundance district) throws something and feeds every time is 2, and the other end (low abundance area) is 1,10 seconds, interval, each Feeding time 20 minutes；So, the total feeding volume in place of safety is 120, and the total feeding volume in hazardous area is 240；With baffle plate, experiment fish is driven to relief area E again after having thrown something and fed, and baffle plate C is reinserted groove gap, with the residual bait of glass siphon sucking-off and feces；After having thrown something and fed the last time, randomly choose 6 tail Siniperca chuatsis (the long 25cm of average body, average weight 260g) one of them big compartment (high abundance-excessive risk) of being respectively put into 6 test cylinder (jar)s processes as the experiment having predation risk, the big compartment of the other end does not put into any Predator (low abundance-devoid of risk), and the big compartment of other 6 cylinders does not put Siniperca chuatsi (low abundance-devoid of risk, high abundance-devoid of risk), process as the experiment without predation risk；
4. measure during behavior observation and record
Continuing above-mentioned mode of throwing something and feeding, every day 2 times (08:00h-11:00h and 14:00h-17:00h), raise and train Oncorhynchi and the feeding activity of wild Oncorhynchi by photographic head record process of throwing something and feeding in 20 minutes, test period is limited to 3 days；So, to each matched group, 6 replications all can be produced；Data are recorded in real time in the hard disk of computer through video frequency collection card, in order to follow-up video recording analysis；
5. behavior quantitative analysis and calculating:
Computer is transferred Video Document, observe and add up following parameter: in 20 minutes of bait throwing in, every 30 seconds as a timing node, record the two kinds of fishes of each timing node individual amount of ingesting in different bait throwing in districts, calculate same gene type Oncorhynchi and participate in the ratio P of feeding activityf=F/4, wherein F is the quantity participating in ingesting；Can judging whether it ingests by some active situation of fish, robbing including scudding eats or go slowly in the position that food falls ingests；The Fish not ingested generally can keep a kind of fixing attitude, unless it is chased by another fish。
Three, measurement result analysis and application example
Based on above research method, to raise and train with wild Oncorhynchi as object of study, it be observed that record statistics, result is as follows:
(1) on the whole, there is significant difference (P < 0.05) in the individual ratio that Oncorhynchi participation that different genotype (raises and train type, wild type) is ingested, and the existence of Predator makes the feeding activity of two kinds of fishes substantially reduce (P < 0.01)；When not having predation risk, the type of raising and train is ingested the ratio P shared by individualityfAverage out to 79.0 ± 5.7%, wild type is 68.7 ± 7.9%, and both do not have significant difference (P > 0.05)；When there being predation risk, the type of raising and train is ingested the ratio average out to 59.0 ± 6.2% shared by individuality, the significantly high 39.8%(P < 0.05 than participating in the wild type (42.2 ± 6.6%) ingested)；
(2) in the experiment having predation risk, no matter it is in low abundance-devoid of risk bait district, or in high abundance-excessive risk bait district, the individual amount raising and train Oncorhynchi is all remarkably higher than the wild type Oncorhynchi in same habitat (P < 0.05)；When predation risk occurs, two kinds of fishes all significantly reduce feeding activity (P < 0.001), but, in high abundance-excessive risk bait district, the Oncorhynchi of raising and train having 5.4 ± 0.7% take part in feeding activity, and the wild Oncorhynchi ingested in this habitat only has 1.1 ± 0.4%, both significant differences (P < 0.05), this shows to raise and train Oncorhynchi and is more willing to risk and ingests, in order to meet the fast-growth demand to energy。
Synthetic data shows: in the experimental system in such a population effect considering Fish and variation habitat, and the type Oncorhynchi of raising and train more gladly takes a risk to ingest；Although predation risk causes that raising and train Oncorhynchi feeding activity is all decreased obviously, but, in high abundance-high risk district of ingesting, the feeding activity raising and train Oncorhynchi is significantly higher than the feeding activity of wild Oncorhynchi；It is to say, it is relatively low to the sensitivity of predation risk to raise and train Fish, more " courageously "；Wild Oncorhynchi is higher to the sensitivity of predation risk, and more " shyly ", it is relatively low that this personal characteristics may result in the survival fitness raising and train Oncorhynchi。Therefore, when artificial releasing through artificial propagation when raising and train Oncorhynchi, tackle its acclimatization training carrying out predation risk, as put into Predator, Oncorhynchi is raised and train in good time disturbance, predation so that it is be transformed into " shyly " Fish by " courageously " Fish, it is thus achieved that more field survivorship technical ability。
Therefore, this method can be applicable to the artificial releasing of Fish:
A, selection individual character are compared with the Fish of " courageously "；
B, the selection place of releasing that food abundance is higher and predation risk is relatively low。
Claims (2)
1. the assay method of a Fish courageously shy behavior, it is characterised in that comprise the following steps:
1. selecting and raising of fish is tested
A, raising and train fish parent in pond culture, wild fish parent of the same race is trapped in field, and the two all cultivates in pond with reference to the cultivation density raising and train fish；
B, mating period synchronize breeding, and opening fry is separately raised in two ponds that environmental condition is identical by different lines；
C, after 30 days, choose the similar type of raising and train of size and wild type and separately support temporarily in two indoor culture cylinders, being uniformly the satiated with food every day frost blood worm twice of throwing something and feeding；
D, maintain this throw something and feed mode until experiment fish schistostoma be suitable for sunk type granular bait for fish of ingesting；
2. individual maximum food consumption and the determination swallowing the time
A, choose 10 similar tails of size and raise and train fish and 10 tail wild fishes, separately raise in 10 glass jars；
B, Therapy lasted 3 days, throw something and feed 2 times, when reaching fed state every day, it is determined that raise and train fish and wild fish is on average ingested the maximum of sunk type granular bait for fish and swallow the time every time；
3. match labelling and adapt to recovery and food abundance and predation risk setting
A, formal first 5 days measured, measure body length to the fish of two strains and weigh；
B, pick out size similar raise and train fish and wild fish forms matched group at random, two kinds of fish quantity of each matched group are equal；
C, labelling and adaptation recover
With the different types of fish in the small plastic of the different colours beads with holes same cylinder of random labelling；
The pairing fish of labelling is transferred in the relief area of determinator and adapt to；
D, food abundance and predation risk set
Determinator is respectively provided with two kinds of experimental conditions:
A, high abundance without Predator or low abundance without Predator；
B, high abundance have Predator or low abundance without Predator；
4. the acquisition of behavioral data
Throwing something and feeding with photographic head record and raise and train the feeding activity of fish and wild fish in process, data are recorded in real time in the hard disk of computer through video frequency collection card, in order to follow-up video recording analysis；
5. behavior quantitative analysis and calculating:
Computer is transferred Video Document, observe and add up following parameter: in 20 minutes of bait throwing in, every 30 seconds as a timing node, record the two kinds of fishes of each timing node individual amount of ingesting in different bait throwing in districts, calculate same gene type fish and participate in the ratio P of feeding activityf=F/4, wherein F is the quantity participating in ingesting。
2. by the application of the courageously assay method of shy behavior of Fish described in claim 1, it is characterised in that be applied to the artificial releasing of Fish:
1. the Fish of individual character relatively " courageously " are selected；
2. the place of releasing that food abundance is higher and predation risk is relatively low is selected。
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| CN201610054518.2A CN105684967A (en) | 2016-01-27 | 2016-01-27 | Fish bold and shy behavior assessment method |
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| CN112314479A (en) * | 2020-11-11 | 2021-02-05 | 北京师范大学 | Monitoring and analyzing method for foraging-refuge balanced behavior of reef fishes |
| CN112753623A (en) * | 2021-01-22 | 2021-05-07 | 交通运输部天津水运工程科学研究所 | Experimental device and testing method for brave and exploration behaviors of fishes |
| CN117770173A (en) * | 2024-01-25 | 2024-03-29 | 长江勘测规划设计研究有限责任公司 | Method for observing fish anti-predation behavior |
| CN118553020A (en) * | 2024-07-26 | 2024-08-27 | 中国海洋大学 | Rainbow trout character classification method based on behaviors |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110089467A (en) * | 2019-04-28 | 2019-08-06 | 中国科学院水生生物研究所 | A kind of device and method of measurement shrimp crab perception feed ability |
| CN112314479A (en) * | 2020-11-11 | 2021-02-05 | 北京师范大学 | Monitoring and analyzing method for foraging-refuge balanced behavior of reef fishes |
| CN112314479B (en) * | 2020-11-11 | 2021-08-31 | 北京师范大学 | Monitoring and analyzing method for foraging-refuge balanced behavior of reef fishes |
| CN112753623A (en) * | 2021-01-22 | 2021-05-07 | 交通运输部天津水运工程科学研究所 | Experimental device and testing method for brave and exploration behaviors of fishes |
| CN112753623B (en) * | 2021-01-22 | 2022-03-29 | 交通运输部天津水运工程科学研究所 | Experimental device and testing method for brave and exploration behaviors of fishes |
| CN117770173A (en) * | 2024-01-25 | 2024-03-29 | 长江勘测规划设计研究有限责任公司 | Method for observing fish anti-predation behavior |
| CN117770173B (en) * | 2024-01-25 | 2024-09-10 | 长江勘测规划设计研究有限责任公司 | Method for observing fish anti-predation behavior |
| CN118553020A (en) * | 2024-07-26 | 2024-08-27 | 中国海洋大学 | Rainbow trout character classification method based on behaviors |
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