CN105678113A - Phenotypic character based approximate variety screening method in peanut specificity test - Google Patents
Phenotypic character based approximate variety screening method in peanut specificity test Download PDFInfo
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- CN105678113A CN105678113A CN201610061376.2A CN201610061376A CN105678113A CN 105678113 A CN105678113 A CN 105678113A CN 201610061376 A CN201610061376 A CN 201610061376A CN 105678113 A CN105678113 A CN 105678113A
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- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
Abstract
The invention discloses a phenotypic character based approximate variety screening method in a peanut specificity test. The method comprises the steps of establishing a peanut variety phenotypic character database at first and then importing numbers, names and character expression state observation values of applied varieties provided by an applicant or character data acquired by a test mechanism into the database; corresponding to an expression state of each character of each applied variety, inputting a corresponding expression state range in the database according to a preset safety value range of a character expression state of a known variety; and screening out approximate varieties meeting the conditions by the database. According to the method, the corresponding expression state selection range of the known variety is flexibly set for different types of characters and different character expression state observation values according to a characteristic that the influence degrees of environmental conditions and personnel to different characters and character expression state observation values are different, so that the omission of the approximate varieties is prevented and the problem of selection of excessive approximate varieties is prevented.
Description
Technical field
The present invention relates to the approximate method for screening varieties in the test of a kind of peanut specificity based on phenotypic character, belong to DUS technical field of measurement and test.
Background technology
Peanut is important oil crops; it is various in style, along with the development of plant breeding and seed industry, and Plant new variety protection (ProtectionofNewVarietiesofPlant; it is called for short PVP) as one of 10 kinds of contents of intellecture property, its importance is more and more outstanding. The prerequisite of new variety authorization and protection is not only in the accurate qualification of new variety, and also for distinguishing fake and forged seed, protection its knowledge property right, safeguards that the vital interests of breeding person provide safeguard and scientific basis. Specificity, consistence and stability are the basic demands to plant variety, are the preconditions that new variety of plant acquisition variety right and main variety of crops are registered by variety certification and non-principal variety of crops.
Specificity refers to that application kind should obviously be different from the applying date all known belonging to together or kind of the same race in the past. For identifying that a kind possesses specificity, it is necessary to prove that this kind is different from known each kind of similar crop on phenotypic character. The known kind of staple crop is often ten hundreds of, each applying for, kind (" application the kind ") kind known to these of DUS test carries out field planting and compare, practice is infeasible. For this reason, need by some Filtering system, those do not need plantation compare can determine to get rid of with the application visibly different kind of kind phenotypic character, only plant those and then do not can not determine by field comparison test and apply for whether kind has the kind of obvious phenotypic difference, be i.e. approximate kind. Tested by field planting, compare and apply for whether kind and approximate kind exist notable difference application kind, show whether application kind possesses the conclusion of specificity accordingly. Therefore, the selection of approximate kind is again the key link of specificity test.
Although the known kind One's name is legion of staple crop, but for most of known kind, the kind of especially early stage seed selection, because the reasons such as yielding ability can not meet current need of production, can not consider when approximate screening varieties.The screening of approximate kind mainly through comparing application kind and still has the trait expression state of the known kind of application potential (kind being such as bred as nearly ten years) to carry out. If can determining that a known kind and the expression status difference of application kind is enough big to such an extent as to inherently showing notable difference between the two during field planting, then can this known kind being got rid of, remaining kind is as approximate kind. For quantitative character and false qualitative character (expression status in the consecutive variations region of false qualitative character), its expression status observed value can present following feature because of the impact of envrionment conditions and human factor: (1) is by environmental influence, the same proterties of same kind, when different year and different areas are planted, can there is certain fluctuation in expression status observed value (expression status code); (2) the degree difference that different trait expressions is affected by environment, shows as expression status degree of fluctuation different because of proterties; (3) different tester is to the observation result of same proterties expression status, it is easy to produce deviation. The size of deviation, had both depended on proterties, also by the impact of expression status present position in character variation scope. Therefore, applying for the expression status observed value of the different proterties of kind according to one, when how to determine approximate screening varieties, known kind expression status span, is the key of approximate screening varieties. Existing way adopts fixing known kind expression status scope: for false qualitative character, adopts expression status identical with it; For quantitative character, adopt the expression status scope differing with it three codes. Adopt fixing known kind expression status scope way, affect bigger proterties and expression status for by environment and personnel's observation, it is easy to omit approximate kind, cause specificity test result unreliable; Otherwise, affect less proterties and expression status for by environment and personnel's observation, cause being selected into too much approximate kind, increase testing cost, reduce test tolerance range.
Summary of the invention
Easily cause omitting or being selected into too much the problem of approximate kind for screening varieties approximate in above-mentioned DUS test, the present invention provides the approximate method for screening varieties in the test of a kind of peanut specificity based on phenotypic character. The method is different according to kind, proterties and trait expression state observe different this feature of influence degree by environment with personnel, by each expression status observed value of application kind being arranged the expression status scope of a corresponding known kind, greatly improve approximate screening varieties efficiency.
The technical scheme of the present invention is: the approximate method for screening varieties in the test of a kind of peanut specificity based on phenotypic character, is characterized in that,
(1) foundation of peanut varieties phenotypic character database
Gather the phenotypic character data (DUS tests whole 31 proterties in guide, and each kind generally tests two growth cycles) of the peanut varieties (kind being such as bred as nearly ten years) currently still with application potential; The phenotypic character data gathered are arranged, sets up peanut varieties phenotypic character database;
(2) apply for that kind trait data imports
Peanut application kind numbering, title and trait expression State Viewpoint measured value (i.e. technology questionnaire trait data) or the trait data of mechanism for testing collection that applicant peanut specificity tested provides import peanut phenotypic character database; Input application variety name (or numbering), the expression status of the correlated character of display application kind;
(3) the expression status scope of known kind is set
The expression status (code) of each application each proterties of kind corresponding, safe span according to the known kind trait expression state set in advance is (as shown in table 1, comprise whole safe spans), input corresponding expression status span (code range) in a database;
(4) approximate screening varieties
Peanut varieties phenotypic character database is by qualified approximate screening varieties out.
The different DUS of table 1 peanut tests the expression status scope that the approximate screening varieties of proterties adopts
Remarks: 1.QN: quantitative character, PQ: false qualitative character; QL: qualitative character;
2. "-" represent there is no (this code) or do not consider (this proterties);
3. " * " represents during DUS tests the proterties specifying to survey.
For the proterties of approximate screening varieties, it is possible to few to several groupings proterties, many to all test guide proterties, how many degree affected by environment with test data of proterties have relation, such as: from the data of same ecotope, affected by environment relatively little, the most multiplex whole test guide proterties; From the test data of planted in different ecological areas, greatly affected by environment, at least adopt whole qualitative characters, on this basis can the stable quantitative character of selection and comparison and false qualitative character.
The present invention expresses according to peanut trait expression mode and proterties and expression status and observes influence degree by environment and personnel, and each application kind expression status observed value corresponding, setting is similar to the known safe span of kind expression status during screening varieties. The safe span of trait expression state sets according to the testing data of multiple years, and the known kind exceeding this scope exists obvious phenotypic difference with application kind, it is not necessary to plant as approximate kind.
The safe span of table 1 of the present invention is according to following rule setting: for qualitative character, it is known that the safe span of kind expression status adopts the expression status identical with application kind, for quantitative character, according to trait expression State Viewpoint measured value by environment and personnel's effect, the safe span of known kind expression status adopts " application kind expression status ± 1 code ", the span of " application kind expression status ± 2 code " or " application kind expression status ± 3 code ", and the difficulty or ease produced according to the position of expression status in range of variation and observed deviation adjust accordingly (when such as applying for kind proterties code for " 1 ", the known safe span of kind expression status is adjusted to "+1 code " by " ± 3 codes "). for false qualitative character, to the expression status being in discontinuous variation region, adopt the mode of qualitative character, to the expression status being in voriability scope, adopt the mode of quantitative character. for the proterties of approximate screening varieties, it is possible to few to several groupings proterties, many to all test guide proterties.
The advantage of the inventive method: the existing approximate method for screening varieties based on phenotypic character, does not fully take into account the impact of different proterties because observing by environment and personnel, the feature that its expression status observed value (code) degree of fluctuation is different. When approximate screening varieties, it is provided with the false qualitative character of fixing known kind and quantitative character expression status scope, it is easy to cause the problem omitting or being selected into too much approximate kind. The method of the present invention, according to envrionment conditions and personnel to different proterties this feature different from the influence degree of trait expression State Viewpoint measured value, for dissimilar proterties and different trait expression State Viewpoint measured values, it is provided with the corresponding expression status range of choice (such as table 1) of known kind flexibly, prevent the omission of approximate kind, it is to increase the preciseness of approximate screening varieties;Avoid because quantity character determination scope is excessive, cause being selected into too much the problem of approximate kind, reduce the cost of specificity test, it is to increase the accuracy of specificity test. By the approximate kind of database technology screening, greatly improve approximate screening varieties efficiency.
Accompanying drawing explanation
Fig. 1 is the approximate screening varieties schematic diagram of peanut application kind 2011-0886A.
Embodiment
Embodiment 1: set up peanut varieties phenotypic character database
2004 to 2014, yellow Huaihai Zone more than 200 part time peanut application kind was carried out DUS test by applicant altogether. Each kind generally tests two growth cycles, in each cycle, whole 31 proterties in test guide is acquired data, gathers phenotype trait data more than 6000 altogether. To the phenotypic character data gathered, arrange according to test proterties phraseology, data type. On this basis, establishing yellow Huaihai Zone peanut varieties phenotypic character database, the DUS storing 192 kinds altogether tests trait data. The screening of the approximate kind of the present embodiment relies on above-mentioned phenotypic character database to carry out.
Embodiment 2: the screening of the approximate kind of peanut
1. peanut application kind totally 6 kinds, code name and title are as shown in table 2;
2. will apply for that kind gathers each trait data (such as table 3) by plantation in a year, import peanut phenotypic character database;
3. click application kind code name, the trait information of display application kind. According to application kind trait expression State Viewpoint measured value, inputting the span (input method is such as Fig. 1) of known kind correlated character expression status, its safe span is as shown in table 1.
4. clicking " screening " button, Database Systems list the approximate kind inventory (such as table 4) of each application kind corresponding.
Kind applied for by table 2
The data (expression status of each proterties, i.e. code value) that kind gathers for 1 year applied for by table 3
| Proterties sequence number | 2011-0886A | 2011-0887A | 2013-0377A | 2013-0434A | 2013-0448A | 2013-1174A |
| 1 | 4 | 4 | 5 | 6 | 6 | 6 |
| 2 | 2 | 2 | 2 | 2 | 2 | 2 5 --> |
| 3 | 3 | 3 | 3 | 3 | 3 | 3 |
| 4 | 2 | 2 | 2 | 2 | 2 | 2 |
| 5 | 4 | 5 | 4 | 5 | 5 | 5 |
| 6 | 2 | 2 | 2 | 2 | 1 | 2 |
| 7 | 1 | 2 | 2 | 2 | 2 | 1 |
| 10 | 4 | 5 | 5 | 5 | 5 | 5 |
| 11 | 1 | 1 | 1 | 1 | 1 | 1 |
| 12 | 1 | 1 | 2 | 2 | 2 | 2 |
| 13 | 3 | 3 | 3 | 3 | 3 | 4 |
| 14 | 5 | 5 | 5 | 4 | 5 | 4 |
| 15 | 2 | 3 | 3 | 3 | 3 | 4 |
| 16 | 3 | 5 | 5 | 5 | 4 | 5 |
| 17 | 1 | 1 | 1 | 1 | 1 | 1 |
| 18 | 3 | 3 | 4 | 4 | 3 | 3 |
| 19 | 2 | 2 | 4 | 5 | 5 | 5 |
| 20 | 5 | 1 | 4 | 6 | 6 | 7 |
| 21 | 5 | 5 | 2 | 3 | 4 | 4 |
| 22 | 3 | 3 | 2 | 3 | 3 | 5 |
| 23 | 2 | 2 | 2 | 2 | 2 | 2 |
| 24 | 3 | 3 | 3 | 3 | 3 | 4 |
| 25 | 7 | 6 | 8 | 7 | 7 | 6 |
| 26 | 4 | 5 | 5 | 7 | 6 | 8 |
| 27 | 2 | 2 | 1 | 2 | 2 | 2 |
| 28 | 1 | 1 | 1 | 1 | 1 | 1 |
| 29 | 4 | 4 | 4 | 4 | 4 | 4 |
| 30 | 2 | 2 | 1 | 1 | 1 | 2 |
| 31 | 1 | 2 | 3 | 1 | 1 | 3 |
Remarks: proterties sequence number is with table 1.
The approximate kind that table 4 filters out
During DUS tests, the approximate kind of application kind and screening to be carried out adjacent plantation, by the observation of application kind and selected approximate this trait expression state of kind is verified, it was demonstrated that the method is suitable, the approximate kind filtered out not only exhaustive but also be not selected into too much approximate kind.
The method of the present invention, especially the span in table 1, to be applicant according to peanut trait expression mode and proterties and expression status express observes influence degree by environment and personnel, testing data according to multiple years verifies the result obtained, and the approximate screening varieties in testing for instructing peanut specificity has important meaning.
Claims (3)
1., based on the approximate method for screening varieties in the peanut specificity test of phenotypic character, it is characterized in that,
(1) foundation of peanut varieties phenotypic character database
Gather the phenotypic character data of the peanut varieties currently still with application potential; The phenotypic character data gathered are arranged, sets up peanut varieties phenotypic character database;
(2) apply for that kind trait data imports
Application kind numbering, title and trait expression State Viewpoint measured value or the trait data of mechanism for testing collection that applicant peanut specificity tested provides import peanut phenotypic character database; Input application variety name or numbering, the expression status of the correlated character of display application kind;
(3) the expression status scope of known kind is set
The expression status of each application each proterties of kind corresponding, according to the safe span of the known kind trait expression state shown in table 1, inputs corresponding expression status span in peanut phenotypic character database;
(4) approximate screening varieties
Peanut varieties phenotypic character database is by qualified approximate screening varieties out;
Table 1 expression status scope
2. as claimed in claim 1 a kind of peanut specificity based on phenotypic character test in approximate method for screening varieties, it is characterized in that, the phenotypic character data of the peanut of described step (1) are that DUS tests whole 31 proterties in guide, and each kind generally tests two growth cycles.
3. as claimed in claim 1 or 2 a kind of peanut specificity based on phenotypic character test in approximate method for screening varieties, it is characterized in that, it is the peanut varieties being bred as in 10 years just pass by that described step (1) currently still has the peanut varieties of application potential.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109409696A (en) * | 2018-10-08 | 2019-03-01 | 南京林业大学 | A kind of cutting flower variety evaluation method and device |
| CN109599150A (en) * | 2018-12-03 | 2019-04-09 | 山东省农业科学院作物研究所 | A kind of peanut specific test approximation method for screening varieties based on phenotype distance |
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| CN103607884A (en) * | 2011-03-17 | 2014-02-26 | 生物探索（新西兰）有限公司 | Screening methods |
| CN104293892A (en) * | 2013-07-16 | 2015-01-21 | 北京林业大学 | Method of detecting phenotypic character related genes in nuclear genome |
| WO2016007632A1 (en) * | 2014-07-08 | 2016-01-14 | Altria Client Services Llc | The genetic locus imparting a low anatabine trait in tobacco and methods of using |
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| CN103607884A (en) * | 2011-03-17 | 2014-02-26 | 生物探索（新西兰）有限公司 | Screening methods |
| CN104293892A (en) * | 2013-07-16 | 2015-01-21 | 北京林业大学 | Method of detecting phenotypic character related genes in nuclear genome |
| WO2016007632A1 (en) * | 2014-07-08 | 2016-01-14 | Altria Client Services Llc | The genetic locus imparting a low anatabine trait in tobacco and methods of using |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109409696A (en) * | 2018-10-08 | 2019-03-01 | 南京林业大学 | A kind of cutting flower variety evaluation method and device |
| CN109409696B (en) * | 2018-10-08 | 2021-05-18 | 南京林业大学 | Cut flower variety evaluation method and device |
| CN109599150A (en) * | 2018-12-03 | 2019-04-09 | 山东省农业科学院作物研究所 | A kind of peanut specific test approximation method for screening varieties based on phenotype distance |
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