CN105676265A

CN105676265A - Sample preparation method of nail electron paramagnetic resonance technical detection for radiation dose estimation

Info

Publication number: CN105676265A
Application number: CN201410658092.2A
Authority: CN
Inventors: 张慧芳; 任越; 李幼忱; 胡波; 段志凯; 党旭红; 刘建功; 王宏力; 杨彪; 张忠新; 王超
Original assignee: China Institute for Radiation Protection
Current assignee: China Institute for Radiation Protection
Priority date: 2014-11-18
Filing date: 2014-11-18
Publication date: 2016-06-15

Abstract

The present invention belongs to the field of the radiation dose measurement technology, and specifically relates to a sample preparation method of nail electron paramagnetic resonance technical detection for radiation dose estimation. The method comprises: a nail sample is cut at a time into the size, wherein the size includes a width of from 1 to 2mm and a length of from 5 to 10mm; a filter paper is wetted by distilled water, and the wetted filter paper is used for rapidly warp cut nail sample; and after the nail sample is naturally dried and used for the electron paramagnetic resonance technology detection. The sample preparation method of nail electron paramagnetic resonance technical detection for radiation dose estimation is able to ensure the uniformity of the loading samples and rapidly eliminate MIS, and has high operability.

Description

The sample preparation methods that a kind of nail the Technique of Electron Paramagnetic Resonance for evaluation of radiation dose detects

Technical field

The invention belongs to radiation dose field of measuring technique, it is specifically related to the sample preparation methods that a kind of nail the Technique of Electron Paramagnetic Resonance for evaluation of radiation dose detects.

Background technology

The potentially dangerous occurred along with popularizing of Application of Nuclear Technology, core and radiation accident is also increasing. Radiation accident personnel are quick, correct dose estimation is the important prerequisite implementing effective treatment, and the quantimeter measured based on EPR (electron paramagnetic resonance) is the effective tool that fast dose is estimated. Radiation-induced free radical is stable in the non-aqueous medias such as tooth, bone, nail, and the amount of radiation-induced stabilized radical becomes positive correlation with dosage, it is possible to detected by EPR. Enamel, bone draw materials limited limit to a great extent its application, and nail samples is collected and it is simple and easy, quick to prepare and painless without wound, external academia has reported for work the research of nail EPR method for dose conversion, and point out its limiting factor mainly with radiation-induced signal (radiationinducedsignal, RIS) there is the machinery that overlapping mechanical induction signal cutting nail causes stress (mechanicallyinducedsignal, and the background signal (backgroundsignal, BKS) of nail self MIS). BKS can be deducted by detection nail background, and MIS needs to be eliminated by certain mode.

Up-to-date result of study shows, MIS is produced by multiple free radical, and time stability is also different, covers RIS to a certain extent. Owing to the nail of Chinese is bigger than the nail camber of westerner, in the sample preparation methods of foreign literature report, the nail sample 1～2mm × 10～20mm size of direct shearing cannot put into the sample hose of maximum 5mm diameter, it is thus desirable to sheared by the nail sample of direct shearing again. In addition, with distilled water immersion, flushing or with moist hairbrush one by one brush sheared edge eliminate the MIS processing mode poor operability caused owing to shearing. Consequently, it is desirable to set up a kind of nail sample processing method for Chinese's nail EPR evaluation of radiation dose, can ensure that the homogeneity filling sample can eliminate again MIS fast, simultaneously workable.

Summary of the invention

It is an object of the invention to for problems of the prior art, it is provided that a kind of can be used for neutron measurement mix boron optical fiber, and use the method for this kind of optical fiber measurement neutron.

It is an object of the invention to provide the sample processing method of a kind of the Technique of Electron Paramagnetic Resonance detection being suitable for utilizing Chinese's nail to carry out evaluation of radiation dose, can ensure that the homogeneity filling sample can eliminate again MIS fast, simultaneously workable.

The technical scheme of the present invention is as follows: the sample preparation methods that a kind of nail the Technique of Electron Paramagnetic Resonance for evaluation of radiation dose detects, and comprises the steps:

(1) nail sample is cut into 1～2mm × 5～10mm size through single time;

(2) qualitative filter paper distilled water is soaked, wrap up the nail sample sheared with the filter paper of humidity fast;

(3), after naturally being dried by nail sample, it is used for the Technique of Electron Paramagnetic Resonance detection as sample.

Further, the sample preparation methods detected for the nail the Technique of Electron Paramagnetic Resonance of evaluation of radiation dose as above, wherein, in step (2), on the filter paper of diameter 10cm, inject 0.5ml distilled water with syringe, distilled water is spread rapidly, realize the wetting of filter paper.

The useful effect of the present invention is as follows: the nail sample collected is cut into 1～2mm × 5～10mm size by the present invention from centre, ensures that nail sample can put into maximum diameter 5mm sample hose, can ensure to fill the homogeneity of sample simultaneously. In addition, wrapped up the nail sample sheared by wetting filter paper fast, the effect with moist hairbrush brush sheared edge that document is reported can be reached, turn avoid and contact water overlong time with distilled water immersion, greatly save the time of operating process simultaneously, workable.

Accompanying drawing explanation

Fig. 1 is the nail sample EPR spectrogram (in figure, X-coordinate is magneticstrength, and ordinate zou is strength of signal) of different cut mode;

In Fig. 1, a is that nail background EPR composes; B shears nail EPR for single time to compose; C repeatedly shears nail EPR to compose, and 3550G place is g standard specimen fignal center.

Fig. 2 is repeatedly shear sample changing conditions spectrogram (in figure, X-coordinate is magneticstrength, and ordinate zou is strength of signal) in time after water treatment;

In Fig. 2 a, b, c, d represent successively nail sample repeatedly shear rear 5min, background, repeatedly shear after the EPR of water treatment immediately and seasoning detect collection of illustrative plates.

Embodiment

Below in conjunction with drawings and Examples, the present invention is described in detail.

The sample preparation methods that nail the Technique of Electron Paramagnetic Resonance for evaluation of radiation dose provided by the present invention detects, comprises the steps:

(1) nail sample is cut into 1～2mm × 5～10mm size through single time;

(2) on the qualitative filter paper of diameter 10cm, inject 0.5ml distilled water with syringe, distilled water is spread rapidly, realize the wetting of filter paper, wrap up the nail sample sheared with the filter paper of humidity fast;

(3), after nail sample being dried naturally, detect for the Technique of Electron Paramagnetic Resonance.

First test the nail sample collected and cut into 1～2mm × 5～10mm size dress sample homogeneity through single time, then the qualitative filter paper of observation humidity wraps up the effect that nail eliminates MIS fast. The nail sample collected is cut into from centre nail sample 1～2mm × 1～2cm size that 1～2mm × 5～10mm size changes document report and cannot put into the fact of maximum diameter 5mm sample hose, can ensure to fill the homogeneity of sample simultaneously. In addition, injecting 0.5mL distilled water with syringe on the filter paper of diameter 10cm, distilled water spreads rapidly, then wraps up the nail sample sheared fast with qualitative filter paper. The effect with moist hairbrush brush sheared edge that document is reported can be reached, turn avoid and contact water overlong time with distilled water immersion, greatly save the time of operating process simultaneously, workable.

The technique effect that the present invention is described specifically is tested below by some.

One, the sample uniformity test that single time is sheared

Getting 3 parts of nail samples, every sheet nail cuts into 1～2mm × 5～10mm size from centre, places and treats that mechanical signal decays completely in 1 day. It is respectively charged into 3 EPR pipes, carries out EPR detection. Sample is poured out and repeats dress sample, duplicate detection, amount to 10 times, record error and be respectively 5.6%, 3.7%, 6.3%, be on average 5.2%. Illustrate and sample is cut into the homogeneity that 1～2mm × 0.5～1cm size can ensure sample.

Two, the impact of MIS is tested by cut mode

Getting 2 parts, the nail sample of same person, one part cuts into 1～2mm × 5～10mm size through single time, and one part through repeatedly shearing 1～2mm × 1～2mm size. Filling sample after shearing and directly carry out EPR detection, next day carries out EPR detection again, and the EPR collection of illustrative plates of nail detection next day is background collection of illustrative plates. As shown in Figure 1, can be observed, the EPR collection of illustrative plates (in figure shown in c) through repeatedly shearing is in obviously bimodal, and its strength of signal is obviously higher than the sample sheared through single time, and deviation background EPR collection of illustrative plates (in figure shown in a) degree is bigger. In order to effectively avoid the impact of MIS, sample process process adopts and shears for single time.

Three, moist filter paper wraps up mode fast and the impact of MIS strength of signal is tested

Owing to nail sample MIS strength of signal after repeatedly shearing is higher, therefore observe moist filter paper and wrap up mode fast to the mode that when affecting, employing is repeatedly sheared of MIS strength of signal.

The nail sample getting same person is through repeatedly cutting into 1～2mm × 1～2mm size. One part directly fills sample and carries out EPR detection, and next day is detected again. Inject the distilled water of 0.5mL on the filter paper of diameter 10cm with syringe, distilled water spreads rapidly, then wraps up fast with filter paper and shear good another part of nail sample, immediately and carry out EPR detection after naturally drying. As shown in Figure 2, can be observed, the sample repeatedly sheared is compared with background, have obvious MIS fignal center at 3525G place, after wrapping up fast with moist filter paper, this fignal center disappears, after seasoning, this peak does not occur again, proves that the mode wrapped up fast with moist filter paper can eliminate major part MIS fast.

Obviously, the present invention can be carried out various change and modification and not depart from the spirit and scope of the present invention by the technician of this area. Like this, if these amendments of the present invention and modification belong within the scope of the claims in the present invention and equivalent technology thereof, then the present invention also is intended to comprise these change and modification.

Claims

1. the sample preparation methods detected for the nail the Technique of Electron Paramagnetic Resonance of evaluation of radiation dose, comprises the steps:

(1) nail sample is cut into 1～2mm × 5～10mm size through single time;

2. the sample preparation methods detected for the nail the Technique of Electron Paramagnetic Resonance of evaluation of radiation dose as claimed in claim 1, it is characterized in that: in step (2), on the filter paper of diameter 10cm, 0.5ml distilled water is injected with syringe, distilled water is spread rapidly, realizes the wetting of filter paper.