CN105675571A - Tiopronin concentration determination fluorescence sensor and preparation method thereof - Google Patents
Tiopronin concentration determination fluorescence sensor and preparation method thereof Download PDFInfo
- Publication number
- CN105675571A CN105675571A CN201610127996.1A CN201610127996A CN105675571A CN 105675571 A CN105675571 A CN 105675571A CN 201610127996 A CN201610127996 A CN 201610127996A CN 105675571 A CN105675571 A CN 105675571A
- Authority
- CN
- China
- Prior art keywords
- tiopronin
- concentration
- optical sensor
- preparation
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a tiopronin concentration determination fluorescence sensor and a preparation method thereof. After hydrotalcite is delaminated, a laminate has positive charges, a guest molecule alizarin red-boric acid system has negative charges, and the hydrotalcite laminate and the guest molecule are self-assembled layer by layer through an electrostatic attraction layer to form a super-molecular structure system and then to be prepared into films; a molecular structure of boric acid is changed by adjusting the layer quantity of the thin films, the concentration of boric acid and the pH value of an alizarin red-boric acid solution system in an assembling process; and a fluorescence sensor thin film with high fluorescence intensity and high stability is obtained through optimized treatment. The fluorescence sensor thin film has the advantages that the preparation method is simple, the cost is low, and quantitative detection of tiopronin is realized. The fluorescence sensor provided by the invention can be used for detecting concentration of tiopronin conveniently and rapidly, and is high in sensitivity and low in detection limit; in the detection process, the sensor does not pollute a system to be detected, and has no reagent loss; and regeneration and repeated utilization of the sensor can be realized.
Description
Technical field
The invention belongs to sensor preparing technical field, measure fluorescent optical sensor and preparation method thereof particularly to a kind of concentration of tiopronin.
Background technology
At biochemical field, tiopronin, as a kind of glycine derivative, has removing toxic substances, protects hepatic tissue, improves the effects such as liver function. But, when over administration, the ill symptomses such as blood pressure drops, accelerated breathing in the short time, can be caused. Therefore, the means of efficient quick detection concentration of tiopronin are the heat subjects of biochemical field research. About the detection of tiopronin, most of producers adopt method and the high performance liquid chromatography of titration, and titrimetry specificity is poor, and high performance liquid chromatography is relatively costly, complicated operation. Development need according to medical industry, more quickly, efficiently, simple operation detection concentration of tiopronin method be in recent years medical treatment, analysis field research direction.
Studies of Fluorescent Film Sensors is a kind of emerging detection technique, obtains the special concern of people in recent years. Comparing traditional detection method, Studies of Fluorescent Film Sensors has the advantage that (1) preparation method is simple, with low cost; (2) can Reusability, do not pollute system to be measured, without reagent consumption; (3) swift to operate, efficient. But, up to the present, the research of Studies of Fluorescent Film Sensors is also very weak, and most researchs are still in conceptual phase. Meanwhile, real device, it is possible to the Studies of Fluorescent Film Sensors being put to practical application is few in number. Therefore, the main contents that existing applied research is the research of future thin film fluorescent optical sensor are expanded.
Alizarin red (ARS) 1,2-dihydroxy-9,10-anthraquinone analog compound, due in molecule and the effect of intermolecular hydrogen bonding make the flexible vibrations of hydrogen cause the non-radiative relaxation process being exceedingly fast, cause its Quenching of fluorescence, therefore ARS itself send extremely weak fluorescence. Boron is very easily with 1,2 and 1, and 3 dihydroxy compounds combine and form stable chelate.And exist in a variety of forms in aqueous, its existence form is limited mainly by the impact of pH value and concentration, it was reported that boric acid is in aqueous mainly with B (OH)3Monomer exists, but has small part B (OH)3Molecule and OH in water-In conjunction with, form B (OH)4 -. When concentration is less than or equal to 0.025M, boric acid is with B (OH)3With B (OH)4 -Form exists. But when higher concentration, trimerization boric acid B can be formed3O3(OH)4 -。
It is a kind of novel multi-functional layered material that LDHs is also called brucite (LayeredDoubleHydroxides is abbreviated as LDHs), and its chemical stability is good, has strong heat resistanceheat resistant performance.
Summary of the invention
It is an object of the invention to provide a kind of method utilizing Supramolecular self assembly and prepare Studies of Fluorescent Film Sensors, and achieve the good fluorescence response of concentration of tiopronin, detection sensitivity is high, efficient quick.
After the present invention utilizes brucite delamination, laminate is positively charged, guest molecule alizarin red-boric acid system is with negative charge, brucite laminate prepares film forming after forming supramolecular structure system with guest molecule by electrostatic attraction LBL self-assembly, and by the number of plies of modulation thin film, the concentration of boric acid, and the pH value of alizarin red-boric acid solution system in assembling process, namely change the molecular structure of boric acid, after being processed by optimization, obtain the fluorescent optical sensor thin film of high fluorescent, high stability. Finally investigate the thin film sensor fluorescence response to variable concentrations tiopronin.
Concentration of tiopronin of the present invention measures the preparation method of fluorescent optical sensor:
A). preparing hydrotalcite precursor, the chemical formula of hydrotalcite precursor is Mg1-xAlx(OH)2(NO3)x·mH2O, wherein 0.2≤x≤0.33, m is water of crystallization quantity, and span is 0.5-9;
B). take the hydrotalcite precursor 0.1-0.2g of preparation in step a), be equipped with in the there-necked flask of 100-200ml Methanamide, at N2The lower stirring reaction 45-48h of protection, obtains the brucite laminate colloid solution after delamination after centrifugal;
C). preparing alizarin red concentration with deionized water is 5.0 × 10-4-1×10-3Mol/L, boric acid concentration is the mixed aqueous solution of 0.15-0.25mol/L, regulates after pH value is 6.5-7.5 with NaOH, keeps in Dark Place in volumetric flask;
D). piezoid carries out pre-assembled in PDDA solution;
E). the piezoid of the pre-assembled PDDA obtained by step d) is placed in the step c) alizarin red prepared and immersion 9-11min in the mixed aqueous solution of boric acid, cleans with deionized water and at room temperature dry up after taking-up; Being subsequently placed in step b) the brucite laminate colloid solution prepared and soak 9-11min, after taking-up, deionized water cleans and at room temperature dries up;
F). repeat step e) 0-39 time, obtain assembling the laminated film of 1-40 layer, be concentration of tiopronin and measure fluorescent optical sensor.
The preparation method of described hydrotalcite precursor is hydrothermal synthesis method.
On described step d) piezoid, the method for pre-assembled PDDA is: by the dense H that piezoid volume ratio is 7:32SO4And H2O2Mixed solution clean, then replace ultrasonic each twice with ethanol and deionized water, each 15-20min, be finally placed in PDDA solution immersion 25-30min, with deionized water rinsing and at room temperature dry up after taking-up, make one layer of positive charge of load in quartz substrate.
The above-mentioned concentration of tiopronin prepared measures fluorescent optical sensor and is applied to quantitative assay tiopronin. Its assay method is: first concentration of tiopronin is measured fluorescent optical sensor and is soaked in CuSO4In solution, after taking-up, detecting its fluorescence intensity, result shows that fluorescent quenching can occur this fluorescent optical sensor;Then, this fluorescent optical sensor is soaked in tiopronin solution, after taking-up, detect its fluorescence intensity, result shows that the fluorescence of this fluorescent optical sensor recovers, tiopronin is in 0-80 μ g/ml concentration range, and its fluorescence recovery value and concentration of tiopronin have linear relationship, thus realizing the detection to concentration of tiopronin.
Concentration of tiopronin prepared by the present invention measures fluorescent optical sensor detection mechanism: copper ion is typical fluorescence quencher, and when adding copper ion, copper ion can be combined with the carboxyl of alizarin red molecule thus causing fluorescence generation quencher; Being subsequently added drug molecule tiopronin, owing to the mercapto in tiopronin molecule has the binding ability higher with copper ion, copper ion can from ARS-Cu2+In discharge, thus the fluorescence of fluorescent optical sensor is recovered. By the fluorescence spectrophotometer test to fluorescence intensity, and then the content of tiopronin can be detected. Successively repeatedly, it is possible to realize concentration of tiopronin and measure reusing of fluorescent optical sensor.
It is an advantage of the current invention that:
1. the preparation method of the fluorescent optical sensor thin film of the present invention is simple, with low cost, it is easy to operation, it is achieved that the quantitative detection to tiopronin.
2. the fluorescent optical sensor that prepared by the present invention is convenient and swift to the Concentration Testing process of tiopronin, highly sensitive, and detection limit is low; In detection process, this sensor does not pollute system to be measured, and without reagent loss; And the regeneration of sensor can be realized and reuse.
3. the present invention introduces boric acid in brucite/alizarin red laminated film, the maximum dispersion of alizarin red molecule can be kept, can effectively suppress the Fluorescence-quenching owing to alizarin red is intermolecular or the flexible vibrations of intramolecular hydrogen bond cause nonradiative relaxation causes, be remarkably improved the optical stability of its fluorescence intensity and fluorescence molecule.
4. the present invention is with brucite for carrier, and thin film can keep the characteristic of brucite itself simultaneously, namely nontoxic, stable, have good biocompatibility, and the inorganic laminate of brucite is not easily aging, it is possible to permanent preservation.
Accompanying drawing explanation
Fig. 1 is Mg in embodiment 1 step 10.67Al0.33(OH)2(CO3)0.33·2H2O brucite and Mg0.67Al0.33(OH)2(NO3)0.33·2H2The X-ray powder diffraction figure of O brucite; Wherein abscissa is 2 θ values, unit: degree; Vertical coordinate is intensity; A is Mg0.67Al0.33(OH)2(CO3)0.33·2H2O brucite, B is Mg0.67Al0.33(OH)2(NO3)0.33·2H2O brucite.
Fig. 2 is the SEM figure of the hydrotalcite precursor of embodiment 1 preparation.
Fig. 3 A figure is the ultraviolet-ray visible absorbing collection of illustrative plates of the tiopronin fluorescence intensity determination sensor of the different numbers of plies of embodiment 1 preparation, wherein illustration is the different thin film linear graphs in 510nm absorption intensity value assembling the number of plies, wherein abscissa is the assembling number of plies of laminated film, and vertical coordinate is the absorption intensity at 510nm place; B is the fluorescence spectrum figure of the tiopronin fluorescence intensity determination sensor of the different numbers of plies of embodiment 1 preparation, wherein illustration is the different thin film linear graphs in 624nm place fluorescence intensity assembling the number of plies, wherein abscissa is the assembling number of plies of thin film, and vertical coordinate is the fluorescence intensity at 624nm place.
Fig. 4 is that under embodiment 1 reaction condition, different boric acid concentrations carry out the 40 layer film sensors assembled, and A is ultraviolet-ray visible absorbing figure; B figure is fluorescence spectrum figure.
Fig. 5 is the fluorescence spectrum figure that the alizarin red of different pH value under embodiment 1 reaction condition and boric acid mixed solution carry out the 40 layer film sensors assembled.
Fig. 6 is the fluorescent optical sensor of 40 layers of the embodiment 1 preparation fluorescence response value to the copper-bath of variable concentrations;Wherein abscissa is wavelength, unit: nM; Vertical coordinate is fluorescent value; From top to bottom the concentration of copper sulfate be followed successively by 0,10,40,80,320,480,720,1500, the fluorescence response value figure of the solution of 3600nM. Illustration is the thin film linear graph in the fluorescence intensity at 624nm place, and abscissa is the concentration of copper sulfate, unit: nM; Vertical coordinate is fluorescence intensity.
The concentration of tiopronin that Fig. 7 is 40 layers of embodiment 1 preparation measures the fluorescent optical sensor fluorescence response value to the tiopronin solution of variable concentrations; Wherein abscissa is wavelength, unit: μ g/ml; Vertical coordinate is fluorescent value; The concentration of copper sulfate is followed successively by 0,10,30,50,70,120 μ g/ml from bottom to up, the fluorescence response value figure of solution. Illustration is the thin film linear graph in the fluorescence intensity at 624nm place, and abscissa is the concentration of tiopronin, unit: μ g/ml; Vertical coordinate is fluorescence intensity.
The concentration of tiopronin that Fig. 8 A is 40 layers of embodiment 1 preparation measures fluorescent optical sensor fluorescence intensity after ultra violet lamp, the i.e. test of fluorescent stability; B is the reperformance test figure of fluorescent optical sensor.
Fig. 9 is the interference test of the concentration of tiopronin mensuration fluorescent optical sensor of 40 layers of embodiment 1 preparation.
Detailed description of the invention
Embodiment 1
1. prepare hydrotalcite precursor: by 0.002mol solid Mg (NO3)2·6H2O, 0.01mol solid Al (NO3)3·9H2O and 0.012mol carbamide is dissolved in the deionized water of 70ml, in the pressure reacting container of the polytetrafluoroethylsubstrate substrate of 90ml, react 24 hours under 100 DEG C of conditions, it is about 7 to pH with deionized water centrifuge washing, dry in atmosphere under 60 DEG C of conditions, obtain the brucite crystallite Mg that carbonate form is anion intercalated0.67Al0.33(OH)2(CO3)0.33·2H2O. Take the anion intercalated brucite crystallite 0.3g of above-mentioned carbonate form and solid NaNO363.75g be dissolved in 300mL except CO2Deionized water in dispersed after, add after 0.08mL concentrated nitric acid at 25 DEG C, stir when nitrogen atmosphere, carry out ion-exchange reactions after 24 hours with except CO2Deionization hot water centrifuge washing to pH be about 7,60 DEG C dry 12 hours, obtain the hydrotalcite precursor of magnalium nitrate anion intercalation, its chemical formula is Mg0.67Al0.33(OH)2(NO3)0.33·2H2O;
2. taking above-mentioned hydrotalcite precursor 0.1g, when nitrogen atmosphere, stir 48 hours in 100mL formamide solvent, mixing speed is 2000 revs/min, by the brucite solution centrifugal after stripping, discards precipitate, obtains clear brucite laminate colloid solution;
3. compound concentration is 10-3The alizarin red of mol/L and the boric acid mixed solution that concentration is 0.2mol/L, regulating pH value with NaOH is after 7, keeps in Dark Place in volumetric flask;
4. take the piezoid of 3cm × 1cm size, with the dense H that volume ratio is 7:32SO4And H2O2Mixed solution clean, then replace ultrasonic 2 times with ethanol and deionized water, each 20min, be then placed in the PDDA solution of 20 times of volumes of dilution and soak 30min, with deionized water rinsing and at room temperature dry up after taking-up; This process is pre-assembled process, makes one layer of positive charge of load in quartz substrate;
5. the piezoid of pre-assembled PDDA step 4 obtained puts into immersion 10min in alizarin red-boric acid mixed solution that step 3 configures, and cleans with deionized water and at room temperature dry up after taking-up; Being subsequently placed in the brucite laminate colloid solution of step 2 preparation and soak 10min, after taking-up, deionized water cleans and at room temperature dries up;
6. respectively repeat steps 5,4,9,14,19,24,29,34,39 times, assembled the laminated film of 5,10,15,20,25,30,35,40 layers respectively, be concentration of tiopronin and measure fluorescent optical sensor.
By UV-vis, XRD, fluorogram it can be seen that be successfully assembled into regular laminated film.
Fluorescent optical sensor performance test:
(1) the fluorescence response value of variable concentrations copper-bath is changed:
Obtained tiopronin fluorescent optical sensor is soaked in the copper-bath that concentration is 10nM, 40nM, 80nM, 320nM, 480nM, 720nM, 1500nM, 3600nM successively; Its fluorescence intensity is surveyed after taking-up. Result shows, along with the increase of concentration of copper sulfate, fluorescence intensity is gradually lowered.
(2) the fluorescent value response value of the tiopronin of variable concentrations is changed:
Tiopronin sensor after fluorescent quenching is soaked in successively in the tiopronin solution that concentration is 10 μ g/ml, 30 μ g/ml, 50 μ g/ml, 70 μ g/ml, 120 μ g/ml, surveys its fluorescence intensity. Result shows, along with the increase of concentration of tiopronin, fluorescence strengthens gradually, and within the scope of 0-80 μ g/ml, it is shown that linear relationship preferably.
It is shown that this concentration of tiopronin measures fluorescent optical sensor, and tiopronin has good selectivity, wider detection range, higher sensitivity, and present linear relationship within the specific limits.
(3) regeneration of sensor and reusing:
The concentration of tiopronin carrying out fluorescence recovery through tiopronin measures fluorescent optical sensor, and again after copper-bath soaks, quencher can occur again fluorescence. Then again by it after tiopronin solution soaking, fluorescence can recover again again, it is thus possible to realize reusing of fluorescent optical sensor.
(4) interference test:
Obtained concentration of tiopronin fluorescent optical sensor is respectively placed in K+, Ca2+, Na+, Mg2+, Al3+, Fe2+, Zn2+, Ag+, Ba2+In solution, after taking-up, survey its fluorescence intensity. Result shows, sensor fluorescence intensity has cancellation in various degree.
Then, above-mentioned fluorescent optical sensor is respectively placed in tiopronin solution, after taking-up, surveys its fluorescence intensity. Result shows, fluorescence intensity has almost no change. Show that tiopronin is had good selectivity by this fluorescence sense.
Embodiment 2
1. with case study on implementation 1;
2. take above-mentioned nitrate anion intercalated houghite 0.11g, when nitrogen atmosphere, stir 47 hours in 110mL formamide solvent, mixing speed is 2500 revs/min, by the brucite solution centrifugal after stripping, discard precipitate, obtain clear brucite laminate colloid solution;
3. compound concentration is 10-3The alizarin red of mol/L and the boric acid mixed solution that concentration is 0.15mol/L, regulating pH value with NaOH is after 6.5, keeps in Dark Place in volumetric flask;
4. take the piezoid of 3cm × 1cm size, with the dense H that volume ratio is 7:32SO4And H2O2Mixed solution clean, then replace ultrasonic 2 times with ethanol and deionized water, each 15min, be then placed in the PDDA solution of 19 times of volumes of dilution and soak 28min, with deionized water rinsing and at room temperature dry up after taking-up; This process is pre-assembled process, makes one layer of positive charge of load in quartz substrate;
5. the piezoid that step 4 obtains is put into immersion 9min in alizarin red-boric acid mixed solution that step 3 is prepared, cleans with deionized water after taking-up and at room temperature dry up; Being subsequently placed in the brucite laminate colloid solution of step 2 preparation and soak 9min, after taking-up, deionized water cleans and at room temperature dries up;
6. repeat step 5,37 times, assemble 38 layers of laminated film, be concentration of tiopronin and measure fluorescent optical sensor.
Embodiment 3
1. with case study on implementation 1;
2. taking above-mentioned nitrate anion intercalated houghite 0.15g, when nitrogen atmosphere, stir 46 hours in 150mL formamide solvent, mixing speed is 2600 revs/min, by the brucite solution centrifugal after stripping, discards precipitate, obtains clear colloid solution.
3. compound concentration is 10-3The alizarin red of mol/L and the boric acid mixed solution that concentration is 0.25mol/L, regulating pH value with NaOH is after 7.5, keeps in Dark Place in volumetric flask;
4. take the piezoid of 3cm × 1cm size, with the dense H that volume ratio is 7:32SO4And H2O2Mixed solution clean, then replace ultrasonic 2 times with ethanol and deionized water, each 18min, be then placed in the PDDA solution of 21 times of volumes of dilution and soak 25min, with deionized water rinsing and at room temperature dry up after taking-up; This process is pre-assembled process, makes one layer of positive charge of load in quartz substrate;
5. the piezoid that step 4 obtains is put into immersion 11min in alizarin red-boric acid mixed solution that step 3 configures, cleans with deionized water after taking-up and at room temperature dry up; Being subsequently placed in the brucite laminate colloid solution of step 2 preparation and soak 11min, after taking-up, deionized water cleans and at room temperature dries up;
6. repeat step 5,34 times, assemble 35 layers of laminated film, be concentration of tiopronin and measure fluorescent optical sensor.
Claims (5)
1. the preparation method that a concentration of tiopronin measures fluorescent optical sensor, it is characterised in that its concrete operation step is:
A). preparing hydrotalcite precursor, the chemical formula of hydrotalcite precursor is Mg1-xAlx(OH)2(NO3)x·mH2O, wherein 0.2≤x≤0.33, m is water of crystallization quantity, and span is 0.5-9;
B). take the hydrotalcite precursor 0.1-0.2g of preparation in step a), be equipped with in the there-necked flask of 100-200ml Methanamide, at N2The lower stirring reaction 45-48h of protection, obtains the brucite laminate colloid solution after delamination after centrifugal;
C). preparing alizarin red concentration with deionized water is 5.0 × 10-4-1×10-3Mol/L, boric acid concentration is the mixed aqueous solution of 0.15-0.25mol/L, regulates after pH value is 6.5-7.5 with NaOH, keeps in Dark Place in volumetric flask;
D). piezoid carries out pre-assembled in PDDA solution;
E). the piezoid of the pre-assembled PDDA obtained by step d) is placed in the step c) alizarin red prepared and immersion 9-11min in the mixed aqueous solution of boric acid, cleans with deionized water and at room temperature dry up after taking-up; Being subsequently placed in step b) the brucite laminate colloid solution prepared and soak 9-11min, after taking-up, deionized water cleans and at room temperature dries up;
F). repeat step e) 0-39 time, obtain assembling the laminated film of 1-40 layer, be concentration of tiopronin and measure fluorescent optical sensor.
2. preparation method according to claim 1, it is characterised in that the preparation method of described hydrotalcite precursor is hydrothermal synthesis method.
3. preparation method according to claim 1, it is characterised in that on described step d) piezoid, the method for pre-assembled PDDA is: by the dense H that piezoid volume ratio is 7:32SO4And H2O2Mixed solution clean, then replace ultrasonic each twice with ethanol and deionized water, each 15-20min, be finally placed in PDDA solution immersion 25-30min, with deionized water rinsing and at room temperature dry up after taking-up, make one layer of positive charge of load in quartz substrate.
4. the concentration of tiopronin that method according to claim 1 prepares measures the application of fluorescent optical sensor quantitative assay tiopronin.
5. application according to claim 4, it is characterised in that its assay method is: first concentration of tiopronin is measured fluorescent optical sensor and is soaked in CuSO4In solution, after taking-up, detecting its fluorescence intensity, result shows that fluorescent quenching can occur this fluorescent optical sensor;Then, this fluorescent optical sensor is soaked in tiopronin solution, after taking-up, detect its fluorescence intensity, result shows that the fluorescence of this fluorescent optical sensor recovers, tiopronin is in 0-80 μ g/ml concentration range, and its fluorescence recovery value and concentration of tiopronin have linear relationship, thus realizing the detection to concentration of tiopronin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610127996.1A CN105675571A (en) | 2016-03-07 | 2016-03-07 | Tiopronin concentration determination fluorescence sensor and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610127996.1A CN105675571A (en) | 2016-03-07 | 2016-03-07 | Tiopronin concentration determination fluorescence sensor and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105675571A true CN105675571A (en) | 2016-06-15 |
Family
ID=56306955
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610127996.1A Pending CN105675571A (en) | 2016-03-07 | 2016-03-07 | Tiopronin concentration determination fluorescence sensor and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105675571A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107044971A (en) * | 2017-03-06 | 2017-08-15 | 北京化工大学 | A kind of cysteine assay ratio fluorescent sensor, preparation method and application |
| CN107543811A (en) * | 2017-09-20 | 2018-01-05 | 江苏理工学院 | A kind of fluorescence analysis of boron and its application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102053079A (en) * | 2010-12-01 | 2011-05-11 | 北京化工大学 | Fluorescent sensor for magnesium ions and preparation method thereof |
| US20130040329A1 (en) * | 2010-04-20 | 2013-02-14 | ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES=CIC biomaGUNE | Analytical applications of enzymatic growth of fluorescent quantum dots |
| CN103163114A (en) * | 2013-03-27 | 2013-06-19 | 北京化工大学 | Fluorescent sensor for determining serum protein concentration and manufacturing method thereof |
| CN103245662A (en) * | 2013-05-20 | 2013-08-14 | 北京化工大学 | F<-> colorimetric sensor and preparation method thereof |
-
2016
- 2016-03-07 CN CN201610127996.1A patent/CN105675571A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130040329A1 (en) * | 2010-04-20 | 2013-02-14 | ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES=CIC biomaGUNE | Analytical applications of enzymatic growth of fluorescent quantum dots |
| CN102053079A (en) * | 2010-12-01 | 2011-05-11 | 北京化工大学 | Fluorescent sensor for magnesium ions and preparation method thereof |
| CN103163114A (en) * | 2013-03-27 | 2013-06-19 | 北京化工大学 | Fluorescent sensor for determining serum protein concentration and manufacturing method thereof |
| CN103245662A (en) * | 2013-05-20 | 2013-08-14 | 北京化工大学 | F<-> colorimetric sensor and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| LAN JIN,ET AL: "Assembly of layered double hydroxide ants ultrathin film and its application as a biosensing material", 《SENSORS AND ACTUATORS B》 * |
| 吴海芹 等: "ARS-硼酸/LDH复合薄膜的可控制备及性能研究", 《中国化学会第九届全国无机化学学术会议论文集—D无机材料化学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107044971A (en) * | 2017-03-06 | 2017-08-15 | 北京化工大学 | A kind of cysteine assay ratio fluorescent sensor, preparation method and application |
| CN107044971B (en) * | 2017-03-06 | 2019-10-29 | 北京化工大学 | A kind of cysteine assay ratio fluorescent sensor, preparation method and application |
| CN107543811A (en) * | 2017-09-20 | 2018-01-05 | 江苏理工学院 | A kind of fluorescence analysis of boron and its application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ahmadijokani et al. | Simultaneous detection and removal of fluoride from water using smart metal-organic framework-based adsorbents | |
| Zeng et al. | Dual-emissive metal–organic framework as a fluorescent “switch” for ratiometric sensing of hypochlorite and ascorbic acid | |
| Zhao et al. | Synthesis of porphyrin-based two-dimensional metal–organic framework nanodisk with small size and few layers | |
| Xu et al. | Eu (III) functionalized Zr-based metal-organic framework as excellent fluorescent probe for Cd2+ detection in aqueous environment | |
| Che et al. | A portable logic detector based on Eu-MOF for multi-target, on-site, visual detection of Eu3+ and fluoride in groundwater | |
| Zhang et al. | Ultrasensitive detection of Cr (VI)(Cr2O72−/CrO42−) ions in water environment with a fluorescent sensor based on metal-organic frameworks combined with sulfur quantum dots | |
| Zhu et al. | A novel sensitive fluorescent probe of S 2 O 8 2− and Fe 3+ based on covalent post-functionalization of a zirconium (iv) metal–organic framework | |
| Wu et al. | Highly selective separation of rare earth elements by Zn-BTC metal–organic framework/nanoporous graphene via in situ green synthesis | |
| Jiang et al. | Eu-MOF and its mixed-matrix membranes as a fluorescent sensor for quantitative ratiometric pH and folic acid detection, and visible fingerprint identifying | |
| Chen et al. | A highly sensitive and multi-responsive Tb-MOF fluorescent sensor for the detection of Pb2+, Cr2O72−, B4O72−, aniline, nitrobenzene and cefixime | |
| Rozveh et al. | Effect of functionalization of metal-organic frameworks on anion sensing | |
| Wu et al. | Metal–organic frameworks (MOFs) as fluorescence sensors: principles, development and prospects | |
| Zhan et al. | Highly fluorescent scandium-tetracarboxylate frameworks: selective detection of nitro-aromatic compounds, sensing mechanism, and their application | |
| Guo et al. | Lanthanide–Organic Frameworks with Uncoordinated Lewis Base Sites: Tunable Luminescence, Antibiotic Detection, and Anticounterfeiting | |
| CN105675571A (en) | Tiopronin concentration determination fluorescence sensor and preparation method thereof | |
| Qin et al. | Ultrathin 2D Eu3+@ Zn-MOF nanosheets: A functional nanoplatform for highly selective, sensitive, and visualized detection of organochlorine pesticides in a water environment | |
| Lu et al. | Multifunctional lanthanide MOFs with active sites as new platforms for smart sensing of methylmalonic acid and anti-counterfeiting applications | |
| Liu et al. | Luminescent porous metal–organic gels for efficient adsorption and sensitive detection of chlortetracycline hydrochloride assisted by smartphones and a test paper-based analytical device | |
| CN108854974A (en) | A kind of difunctional core-shell nano flower composite material of intelligence and preparation method and application | |
| Shao et al. | A Mn-based LMOF with an AIEgens ligand for selective detection of Fe3+, CrO42− and Cr2O72− ions in aqueous solution | |
| Zhang et al. | Co2+ and nitrobenzene sensing using indium-based metal-organic framework | |
| CN106001598A (en) | Method for synthesizing piece-shaped gold nanoparticles through hydrotalcite interlamination | |
| You et al. | Cage Bismuth Metal–Organic Framework Materials Based on a Flexible Triazine–Polycarboxylic Acid: Subgram Synthesis, Application for Sensing, and White Light Tuning | |
| Wen et al. | Wavelength-shift-based visual fluorescence sensing of aspartic acids using Eu/Gd-MOF through pH triggering | |
| CN107044971B (en) | A kind of cysteine assay ratio fluorescent sensor, preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160615 |
|
| RJ01 | Rejection of invention patent application after publication |