CN105671122A - Bacteria identifying method and device - Google Patents

Bacteria identifying method and device Download PDF

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CN105671122A
CN105671122A CN201610188737.XA CN201610188737A CN105671122A CN 105671122 A CN105671122 A CN 105671122A CN 201610188737 A CN201610188737 A CN 201610188737A CN 105671122 A CN105671122 A CN 105671122A
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colony
identified
bacterium colony
image
bacterium
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李辰
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Priority to PCT/CN2016/102094 priority patent/WO2017166778A1/en
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    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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    • G06V20/00—Scenes; Scene-specific elements
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Abstract

The invention provides a bacteria identifying method and device. The method comprises the following steps: obtaining an image of a to-be-identified bacterial colony; extracting the bacterial colony feature information from the image of the to-be-identified bacterial colony; and determining the strain information corresponding to the to-be-identified bacterial colony according to the extracted bacterial colony feature information and a pre-established bacterial colony feature library. In the invention, non-contact strain identification of the bacterial colony is realized, and the safety of the identifying personnel is improved; the bacteria identifying method and device impose very low requirements on the professionality of the identifying personnel in bacteria and medical treatment; the identifying process can be quickly finished and the identifying result can be quickly obtained through an automatic information processing process, the identifying efficiency is greatly improved, and the accuracy of the identifying result is very high; and moreover, the image of the to-be-identified bacterial colony can be obtained through multiple ways, the cost of the bacteria identifying device is very low, and thus the identifying cost is remarkably lowered.

Description

Method for determining bacteria and device
Technical field
The present invention relates to image processing techniques and Bacteria Identification technical field, specifically, it relates to a kind of method for determining bacteria and device.
Background technology
At present, in hospital, R&D institution and school, due to objects such as treatment or researchs, it is often necessary to carry out Bacteria Identification, identified the kind of this bacterium by certain method for determining bacteria, with requirements such as satisfied treatment, research or teaching.
Currently, conventional method for determining bacteria, identifies bacterium mainly through the method for biochemistry according to the physiological activity feature of bacterium self, before qualification, bacterium is divided pure with substratum, obtain pure bacterium colony, then get the biochemical pipe of bacterium colony puncture, observe the change of its color, concrete bacterium colony kind is judged with this, or bacterium colony is made suspension, according to special identification card and specialized analyzer, suspension is identified, it is determined that bacterium colony kind.
More than by biochemical method qualification bacterium colony kind, need bacterium colony can make suspension or bacterium colony can fully scatter in a liquid, bacterium colony purity is required very high, the authenticate technology of appraiser is required very high, and in qualification process, when appraiser touches bacterium, there is certain security risk, thus cause Bacteria Identification complexity, cost height and there is potential safety hazard.
Summary of the invention
In view of this, the object of the embodiment of the present invention is to provide a kind of method for determining bacteria and device, it is achieved by the mode of noncontact formula, bacterium colony is carried out strain identification, it is to increase the security of appraiser; This method for determining bacteria and device are very low to the professional requirement of the bacterium of appraiser and medical treatment aspect; Qualification process can be completed fast by the information process of automatization, and obtain qualification result fast, greatly improve determination rates, and qualification result accuracy is very high; Can obtaining the image of bacterium colony to be identified in several ways, Bacteria Identification device expense is very low, thus greatly reduces appraisal cost.
First aspect, embodiments provides a kind of method for determining bacteria, and described method comprises:
Obtain the image of bacterium colony to be identified;
Colony characteristics information is extracted from the image of described bacterium colony to be identified;
According to the described colony characteristics information extracted and the colony characteristics storehouse set up in advance, it is determined that the bacterial classification information that described bacterium colony to be identified is corresponding.
In conjunction with first aspect, embodiments provide the first possible implementation of above-mentioned first aspect, wherein, described from the image of described bacterium colony to be identified, extract colony characteristics information, comprising:
From the image of described bacterium colony to be identified, extract size, color, shape, the glossiness of described bacterium colony to be identified and do wet degree;
Size according to described bacterium colony to be identified, color, shape, glossiness and dry wet degree generate the colony characteristics information of described bacterium colony to be identified.
In conjunction with first aspect, embodiments provide the 2nd kind of possible implementation of above-mentioned first aspect, wherein, described according to the described colony characteristics information extracted and the colony characteristics storehouse set up in advance, determine to comprise the bacterial classification information that described bacterium colony to be identified is corresponding:
From the described colony characteristics storehouse set up in advance, it is determined that go out the colony characteristics the highest with the colony characteristics information matches degree of described bacterium colony to be identified;
By bacterial classification information corresponding for colony characteristics the highest for described matching degree, it is determined that be the bacterial classification information that described bacterium colony to be identified is corresponding.
In conjunction with first aspect, embodiments providing the third possible implementation of above-mentioned first aspect, wherein, the image of described acquisition bacterium colony to be identified, comprising:
The bacterium colony picture collection device carried by terminal gathers the image of bacterium colony to be identified, or the image of the bacterium colony to be identified of the device transmission received except described terminal, or transfers the image of the bacterium colony to be identified of storage from this locality.
In conjunction with first aspect, embodiments providing the 4th kind of possible implementation of above-mentioned first aspect, wherein, described method also comprises:
The colony characteristics that the bacterial classification information that acquisition various species bacterium colony is corresponding, described bacterial classification information bacterium colony kind and described bacterium colony kind are corresponding;
The bacterial classification information corresponding according to described various species bacterium colony sets up colony characteristics storehouse.
Second aspect, embodiments provides a kind of Bacteria Identification device, and described device comprises:
First acquisition module, for obtaining the image of bacterium colony to be identified;
Extraction module, for extracting colony characteristics information from the image of described bacterium colony to be identified;
Determination module, for according to the described colony characteristics information extracted and the colony characteristics storehouse set up in advance, it is determined that the bacterial classification information that described bacterium colony to be identified is corresponding.
In conjunction with second aspect, embodiments providing the first possible implementation of above-mentioned second aspect, wherein, described extraction module comprises:
Extraction unit, for extracting size, color, shape, the glossiness of described bacterium colony to be identified and do wet degree from the image of described bacterium colony to be identified;
Generate unit, generate the colony characteristics information of described bacterium colony to be identified for the size according to described bacterium colony to be identified, color, shape, glossiness and dry wet degree.
In conjunction with second aspect, embodiments providing the 2nd kind of possible implementation of above-mentioned second aspect, wherein, described determination module comprises:
First determining unit, for from the described colony characteristics storehouse set up in advance, it is determined that go out the colony characteristics the highest with the colony characteristics information matches degree of described bacterium colony to be identified;
2nd determining unit, for by bacterial classification information corresponding for colony characteristics the highest for described matching degree, it is determined that is the bacterial classification information that described bacterium colony to be identified is corresponding.
In conjunction with second aspect, embodiments providing the third possible implementation of above-mentioned second aspect, wherein, described first acquisition module comprises:
Collecting unit, the bacterium colony picture collection device for being carried by terminal gathers the image of bacterium colony to be identified;
Receive unit, for the image of the bacterium colony to be identified of device transmission received except described terminal;
Transfer unit, for transferring the image of the bacterium colony to be identified of storage from this locality.
In conjunction with second aspect, embodiments providing the 4th kind of possible implementation of above-mentioned second aspect, wherein, described device also comprises:
2nd acquisition module, for obtaining bacterial classification information corresponding to various species bacterium colony, described bacterial classification information bacterium colony kind and colony characteristics corresponding to described bacterium colony kind;
Set up module, set up colony characteristics storehouse for the bacterial classification information corresponding according to described various species bacterium colony.
In embodiments of the present invention, method for determining bacteria comprises: the image obtaining bacterium colony to be identified; Colony characteristics information is extracted from the image of described bacterium colony to be identified; According to the described colony characteristics information extracted and the colony characteristics storehouse set up in advance, it is determined that the bacterial classification information that described bacterium colony to be identified is corresponding; Bacteria Identification device comprises the first acquisition module, for obtaining the image of bacterium colony to be identified; Extraction module, for extracting colony characteristics information from the image of described bacterium colony to be identified; Determination module, for according to the described colony characteristics information extracted and the colony characteristics storehouse set up in advance, it is determined that the bacterial classification information that described bacterium colony to be identified is corresponding. Bacterium colony carries out strain identification by the mode by noncontact formula that achieves, it is to increase the security of appraiser; This method for determining bacteria and device are very low to the professional requirement of the bacterium of appraiser and medical treatment aspect; Qualification process can be completed fast by the information process of automatization, and obtain qualification result fast, substantially increase determination rates, and qualification result accuracy is very high; Can obtaining the image of bacterium colony to be identified in several ways, Bacteria Identification device expense is very low, thus greatly reduces appraisal cost.
For making above-mentioned purpose, the feature and advantage of the present invention become apparent, better embodiment cited below particularly, and coordinate appended accompanying drawing, it is described in detail below.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme of the embodiment of the present invention, it is briefly described to the accompanying drawing used required in embodiment below, it is to be understood that, the following drawings illustrate only some embodiment of the present invention, therefore should not be counted as is the restriction to scope, for those of ordinary skill in the art, under the prerequisite not paying creative work, it is also possible to obtain other relevant accompanying drawings according to these accompanying drawings.
Fig. 1 shows the schema of a kind of method for determining bacteria that the embodiment of the present invention 1 provides;
Fig. 2 shows the structural representation of a kind of Bacteria Identification device that the embodiment of the present invention 2 provides.
Embodiment
Below in conjunction with accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is only the present invention's part embodiment, instead of whole embodiments. The assembly of the embodiment of the present invention usually describing in accompanying drawing herein and illustrating can be arranged with various different configuration and design. Therefore, below to the detailed description of the embodiments of the invention provided in the accompanying drawings and the scope of the claimed the present invention of not intended to be limiting, but only represent the selected embodiment of the present invention. Based on embodiments of the invention, other embodiments all that those skilled in the art obtain under the prerequisite not making creative work, all belong to the scope of protection of the invention.
Consider in relevant authenticate technology, by the method for biochemistry qualification bacterium colony kind, need bacterium colony can make suspension or bacterium colony can fully scatter in a liquid, bacterium colony purity is required very high, the authenticate technology of appraiser is required very high, and in qualification process, when appraiser touches bacterium, there is certain security risk, thus cause Bacteria Identification complexity, cost height and there is potential safety hazard. Based on this, the present invention provides a kind of method for determining bacteria and device, it is achieved by the mode of noncontact formula, bacterium colony is carried out strain identification, it is to increase the security of appraiser; This method for determining bacteria and device are very low to the professional requirement of the bacterium of appraiser and medical treatment aspect; Qualification process can be completed fast by the information process of automatization, and obtain qualification result fast, greatly improve determination rates, and qualification result accuracy is very high; Can obtaining the image of bacterium colony to be identified in several ways, Bacteria Identification device expense is very low, thus greatly reduces appraisal cost. It is described below by embodiment.
Embodiment 1
See Fig. 1, embodiments provide a kind of method for determining bacteria. The method comprises following S103-S105 step.
In embodiments of the present invention, before obtained the image of bacterium colony to be identified by S103 step, first need to set up colony characteristics storehouse by following S101-S102 step.
The colony characteristics that step S101: obtain the bacterial classification information that various species bacterium colony is corresponding, bacterial classification information bacterium colony kind and bacterium colony kind are corresponding;
Step S102: the bacterial classification information corresponding according to various species bacterium colony sets up colony characteristics storehouse.
In above-mentioned steps S101, according to biology with medically, the classification of bacterium colony can be obtained bacterial classification information corresponding to various species bacterium colony. Above-mentioned bacterial classification information also comprises living environment and the activity information of various species bacterium colony. Wherein, the colony characteristics in bacterial classification information comprises size, color, shape, the glossiness of bacterium colony and does wet degree; Bacterium colony kind and colony characteristics one_to_one corresponding.
After determining the colony characteristics that various species bacterium colony is corresponding, such that it is able to set up the one-to-one relationship of bacterium colony kind and colony characteristics, namely the bacterial classification information of various species bacterium colony is set up, by the bacterial classification information of this various species bacterium colony set up stored in corresponding database, using comprise the bacterial classification information of various species bacterium colony database as colony characteristics storehouse.
After setting up colony characteristics storehouse, identify the bacterial classification of bacterium colony to be identified by following S103-S105 step.
Step S103: the image obtaining bacterium colony to be identified;
Step S104: extract colony characteristics information from the image of bacterium colony to be identified;
Step S105: according to the colony characteristics information extracted and the colony characteristics storehouse set up in advance, it is determined that the bacterial classification information that bacterium colony to be identified is corresponding.
Before obtained the image of bacterium colony to be identified by above-mentioned steps S103, need first to be carried out by this bacterium colony to be identified by substratum point pure, point pure object is the consistent bacterium colony of the bacterium colony in order to obtain the reference culture with relevant bacteria species, so that identifying the bacterium colony kind of this bacterium colony to be identified. In embodiments of the present invention, the purity of the bacterium colony to be identified after point pure is required very low, is suitable for the qualification of most of bacterium colony.
Bacterium colony to be identified is carried out point pure after can by following three kinds of modes in any one mode obtain the image of bacterium colony to be identified.
First kind of way: the bacterium colony picture collection device carried by terminal gathers the image of bacterium colony to be identified.
Above-mentioned terminal can be the mobile terminal such as mobile phone or panel computer, and above-mentioned bacterium colony picture collection device can be the camera being arranged on above-mentioned mobile terminal; Above-mentioned bacterium colony picture collection device can also be CMOS (ComplementaryMetalOxideSemiconductor, complementary metal oxide semiconductor) photographic camera. The image of this bacterium colony to be identified, by the image of shooting bacterium colony to be identified, is transferred to bacterium colony evaluation apparatus by this mobile terminal or CMOS camera automatically.
2nd kind of mode: the image receiving the bacterium colony to be identified of the device transmission except terminal.
Above-mentioned equipment except terminal can be the mobile terminal such as mobile phone, panel computer, it is also possible to is the fixed terminals such as desktop computer. The Colony hybridization to be identified taken in advance is transferred to bacterium colony evaluation apparatus by wired, wireless or transmission interface by this mobile terminal or fixed terminal.
The third mode: the image transferring the bacterium colony to be identified of storage from this locality.
Before bacterium colony to be identified is identified, it is possible in advance the image of the bacterium colony to be identified gathered is stored in local storer, when certain bacterium colony is identified by needs, it is possible to transfer the image of this bacterium colony to be identified from local storer.
Got the image of bacterium colony to be identified by above method after, it is possible to extract colony characteristics information by above-mentioned steps S104 from the image of bacterium colony to be identified, specifically extract by following S1041-S1042 step.
Step S1041: extract size, color, shape, the glossiness of bacterium colony to be identified from the image of bacterium colony to be identified and do wet degree;
Step S1042: the colony characteristics information generating bacterium colony to be identified according to the size of bacterium colony to be identified, color, shape, glossiness and dry wet degree.
In above-mentioned steps S1041, extract the size of bacterium colony to be identified, first the position of bacterium colony to be identified in the image of this bacterium colony to be identified is needed to locate, then determine, according to the position of this bacterium colony to be identified, the region that bacterium colony to be identified is shared in the picture, and calculate the area in this region, the size of bacterium colony to be identified is determined by the area calculated.
Extract the color of bacterium colony to be identified, first the region that bacterium colony to be identified is shared in the picture is determined, then the pixel value of each pixel in this region is counted, and pixel value is converted to corresponding numerical value, the probability of the last numerical value corresponding according to each pixel, color comparison card and each pixel distribution, it is determined that go out the color of bacterium colony to be identified. Wherein, color comparison card comprises the one-to-one relationship of each color and numerical value.
Extract the shape of bacterium colony to be identified, first determine the region that bacterium colony to be identified is shared in the picture, then to be measured the boundary line in this region, determine, according to measuring result and shape computational algorithm, the shape that this bacterium colony to be identified is corresponding.
Extract the glossiness of bacterium colony to be identified, first the region that bacterium colony to be identified is shared in the picture is determined, then contrast gradient and the bright-dark degree of image in this region is calculated, finally according to the contrast gradient calculated, bright-dark degree and image gloss judging criterion, it is determined that go out the glossiness of bacterium colony to be identified.
Extract the dry wet degree of bacterium colony to be identified, first determine then to calculate the reflectance of image in this region in the region that bacterium colony to be identified is shared in the picture, according to the reflectance calculated and dry wet degree synopsis, it is determined that go out the dry wet degree of this bacterium colony to be identified; Wherein, dry wet degree synopsis comprises reflective to the one-to-one relationship with dry wet degree.
In embodiments of the present invention, extract size, color, shape, the glossiness of bacterium colony to be identified and do the characteristics of image such as wet degree, by in conventional images process technology, the image feature extraction techniques such as the size of image, color, shape, glossiness and dry wet degree can also be realized.
Extracted the colony characteristics information of bacterium colony to be identified by above method after, by above-mentioned steps S105, according to the colony characteristics information extracted and the colony characteristics storehouse set up in advance, it is determined that the bacterial classification information that bacterium colony to be identified is corresponding, determine especially by following S1051-S1052 step.
Step S1051: from the colony characteristics storehouse set up in advance, it is determined that go out the colony characteristics that the colony characteristics information matches degree with bacterium colony to be identified is the highest;
Step S1052: by bacterial classification information corresponding for colony characteristics the highest for matching degree, it is determined that be the bacterial classification information that bacterium colony to be identified is corresponding.
In above-mentioned steps S1051, when determining that the colony characteristics information matches with bacterium colony to be identified is spent, first need to compare each colony characteristics in the colony characteristics information of bacterium colony to be identified and this colony characteristics storehouse, wherein, by each feature in the colony characteristics information of bacterium colony to be identified and in colony characteristics storehouse each feature in each colony characteristics compare, comprise the bacterium colony size in the size of comparison bacterium colony to be identified and each colony characteristics; Colony colour in the color of comparison bacterium colony to be identified and each colony characteristics; Colony shape in the shape of comparison bacterium colony to be identified and each colony characteristics; Bacterium colony glossiness in the glossiness of comparison bacterium colony to be identified and each colony characteristics; And the dry wet degree of the dry bacterium colony wet in degree and each colony characteristics of comparison bacterium colony to be identified. Then, the quantity of the characteristic item that is consistent of this colony characteristics is added up according to the comparison result of the various features in each colony characteristics in colony characteristics storehouse. Wherein, the data that comparison and statistics obtain are schematically as shown in table 1 below.
Table 1
Finally, by colony characteristicses maximum for the characteristic item that is consistent counted, it is determined that be the colony characteristics the highest with the matching degree of the colony characteristics of bacterium colony to be identified.
After determining the colony characteristics the highest with the colony characteristics matching degree of bacterium colony to be identified, it is possible to by above-mentioned steps S1052, by bacterial classification information corresponding for colony characteristics the highest for matching degree, it is determined that be the bacterial classification information that bacterium colony to be identified is corresponding.
In embodiments of the present invention, when determining that the colony characteristics information matches with bacterium colony to be identified is spent, if when in colony characteristics storehouse, the matching degree of the colony characteristics information of all colony characteristicses and bacterium colony to be identified is all lower than default matching degree, the bacterial classification going out bacterium colony to be identified now accurately cannot determined according to the colony characteristics in colony characteristics storehouse, also need the concise and to the point Biochemical Information supplementing the corresponding bacterium of qualification bacterium colony to be identified in conjunction with corresponding authenticate technology, the concise and to the point Biochemical Information of the corresponding bacterium of the bacterium colony to be identified according to supplementary qualification determines the bacterial classification of bacterium colony to be identified further, when after the bacterial classification finally determining this bacterium colony to be identified, can according to the bacterial classification determined, the concise and to the point Biochemical Information supplementing the corresponding bacterium of bacterium colony to be identified of qualification generates the bacterial classification information of this bacterium colony to be identified, and this bacterial classification information can be added to colony characteristics storehouse, for the qualification of follow-up bacterium colony to be identified provides appraisal basis, thus the determination rates of bacterium colony can also be improved.
In embodiments of the present invention, can also stored in a large amount of Colony hybridization in above-mentioned colony characteristics storehouse, the corresponding unique bacterial classification information of each Colony hybridization. When it needs to be determined that during the bacterial classification information of bacterium colony to be identified, first obtain the image of bacterium colony to be identified, then extract colony characteristics information from the image of bacterium colony to be identified; Finally according to the Colony hybridization in the colony characteristics information extracted and colony characteristics storehouse, it is determined that the bacterial classification information that bacterium colony to be identified is corresponding.
Above-mentioned according to extract colony characteristics information and colony characteristics storehouse in Colony hybridization, it is determined that the bacterial classification information that bacterium colony to be identified is corresponding, it is possible to realized by following process.
From in the Colony hybridization colony characteristics storehouse, it is determined that go out the Colony hybridization that the colony characteristics information matches degree with bacterium colony to be identified is the highest; By bacterial classification information corresponding for Colony hybridization the highest for matching degree, it is determined that be the bacterial classification information that described bacterium colony to be identified is corresponding.
Above-mentioned bacterial classification information bacterium colony kind and colony characteristics corresponding to bacterium colony kind. When determining the highest Colony hybridization of the colony characteristics information matches degree with bacterium colony to be identified, first the colony characteristics of all Colony hybridization in colony characteristics storehouse is needed to extract, then the colony characteristics of the colony characteristics information of bacterium colony to be identified and each Colony hybridization is compared, wherein, each feature in the colony characteristics of each feature in the colony characteristics information of bacterium colony to be identified and each Colony hybridization is compared, comprises the bacterium colony size in the size of comparison bacterium colony to be identified and the colony characteristics of each Colony hybridization; Colony colour in the color of comparison bacterium colony to be identified and the colony characteristics of each Colony hybridization; Colony shape in the shape of comparison bacterium colony to be identified and the colony characteristics of each Colony hybridization; Bacterium colony glossiness in the glossiness of comparison bacterium colony to be identified and the colony characteristics of each Colony hybridization; And the dry wet degree of the bacterium colony in the colony characteristics of the dry wet degree of comparison bacterium colony to be identified and each Colony hybridization. Then, the quantity of the characteristic item that is consistent of this colony characteristics is added up according to the comparison result of the various features in the colony characteristics of each Colony hybridization. Wherein, the data that comparison and statistics obtain are schematically as shown in Table 1.
Finally, by colony characteristicses maximum for the characteristic item that is consistent counted, it is determined that be the colony characteristics the highest with the matching degree of the colony characteristics of bacterium colony to be identified.
After determining the colony characteristics the highest with the colony characteristics matching degree of bacterium colony to be identified, according to the corresponding relation of Colony hybridization and bacterial classification information, by bacterial classification information corresponding for the Colony hybridization comprising the highest colony characteristics of matching degree, it is determined that be the bacterial classification information that bacterium colony to be identified is corresponding.
In embodiments of the present invention, when identifying the bacterial classification information of bacterium colony to be identified, only need to obtaining the image of bacterium colony to be identified, appraiser does not need actual contact bacterium colony to be identified, avoid and the bacteriological infection that bacterium colony to be identified contacts and cause, thus improve the security of appraiser; In whole qualification process, appraiser does not need to be grasped very professional bacterium and medical knowledge, it is only necessary to grasp the operation of relevant equipment and related software, and the professional requirement of appraiser is very low; The bacterial classification information of bacterium colony is identified by method provided by the invention, the image of bacterium colony to be identified can be got fast, and complete qualification process fast by the information processing device of automatization and software, therefore qualification result can be obtained fast, substantially increase determination rates, and qualification result accuracy is very high; In the present invention, the image of bacterium colony to be identified can be obtained in several ways, whole qualification process does not need the expense that bacterium colony to be identified carries out the payments such as preservation, transport and management, the required expense paid of appraiser is also very low, the cost of Bacteria Identification device is very low, thus greatly reduces appraisal cost.
In embodiments of the present invention, bacterium colony authentication method comprises the image obtaining bacterium colony to be identified; Colony characteristics information is extracted from the image of bacterium colony to be identified; According to the colony characteristics information extracted and the colony characteristics storehouse set up in advance, it is determined that the bacterial classification information that bacterium colony to be identified is corresponding.Bacterium colony carries out strain identification by the mode by noncontact formula that achieves, it is to increase the security of appraiser; This method for determining bacteria and device are very low to the professional requirement of the bacterium of appraiser and medical treatment aspect; Qualification process can be completed fast by the information process of automatization, and obtain qualification result fast, substantially increase determination rates, and qualification result accuracy is very high; Can obtaining the image of bacterium colony to be identified in several ways, Bacteria Identification device expense is very low, thus greatly reduces appraisal cost.
Embodiment 2
See Fig. 2, embodiments providing a kind of Bacteria Identification device, this device comprises:
First acquisition module S1, for obtaining the image of bacterium colony to be identified;
Extraction module S2, for extracting colony characteristics information from the image of bacterium colony to be identified;
Determination module S3, for according to the colony characteristics information extracted and the colony characteristics storehouse set up in advance, it is determined that the bacterial classification information that bacterium colony to be identified is corresponding.
In embodiments of the present invention, obtained the image of bacterium colony to be identified by above-mentioned first acquisition module S1 before, first need by following 2nd acquisition module and set up module and set up colony characteristics storehouse.
Said apparatus also comprises the 2nd acquisition module and sets up module;
2nd acquisition module, for obtaining bacterial classification information corresponding to various species bacterium colony, bacterial classification information bacterium colony kind and colony characteristics corresponding to bacterium colony kind;
Set up module, set up colony characteristics storehouse for the bacterial classification information corresponding according to various species bacterium colony.
Above-mentioned acquisition module, can obtain bacterial classification information corresponding to various species bacterium colony according to biology with medically to the classification of bacterium colony. Above-mentioned bacterial classification information also comprises living environment and the activity information of various species bacterium colony. Wherein, the colony characteristics in bacterial classification information comprises size, color, shape, the glossiness of bacterium colony and does wet degree; Bacterium colony kind and colony characteristics one_to_one corresponding.
After above-mentioned 2nd acquisition module determines the colony characteristics that various species bacterium colony is corresponding, above-mentioned set up module, the colony characteristics that the various species bacterium colony can determined according to the 2nd acquisition module is corresponding, set up the one-to-one relationship of bacterium colony kind and colony characteristics, namely the bacterial classification information of various species bacterium colony is set up, by the bacterial classification information of this various species bacterium colony set up stored in corresponding database, using comprise the bacterial classification information of various species bacterium colony database as colony characteristics storehouse.
Set up after module sets up colony characteristics storehouse when above-mentioned, when needing the bacterial classification information identifying bacterium colony to be identified, need first to be carried out by this bacterium colony to be identified by substratum point pure, pure object is divided to be in order to the bacterium colony consistent with the bacterium colony of the reference culture of relevant bacteria species, so that identifying the bacterium colony kind of this bacterium colony to be identified. In embodiments of the present invention, the purity of the bacterium colony to be identified after point pure is required very low, is suitable for the qualification of most of bacterium colony.
When bacterium colony to be identified is carried out point pure after, above-mentioned first acquisition module S1 can by following collecting unit, receive unit or transfer the image that unit obtains this bacterium colony to be identified.
Above-mentioned first acquisition module S1 comprises collecting unit, receives unit or transfer unit;
Collecting unit, the bacterium colony picture collection device for being carried by terminal gathers the image of bacterium colony to be identified;
Receive unit, for the image of the bacterium colony to be identified of device transmission received except terminal,
Transfer unit, for transferring the image of the bacterium colony to be identified of storage from this locality.
Above-mentioned collecting unit can be the mobile terminal such as mobile phone or panel computer being embedded with camera, it is also possible to be CMOS camera.The image of this bacterium colony to be identified, by the image of shooting bacterium colony to be identified, is transferred to bacterium colony evaluation apparatus by this collecting unit automatically.
Above-mentioned equipment except terminal can be the mobile terminal such as mobile phone, panel computer, it is also possible to is the fixed terminals such as desktop computer. The Colony hybridization to be identified taken in advance is transferred to above-mentioned reception unit by wired, wireless or transmission interface by this mobile terminal or fixed terminal.
Before being identified by bacterium colony to be identified, it is possible to be in advance stored in local storer by the image of the bacterium colony to be identified gathered, when certain bacterium colony is identified by needs, above-mentioned transfer the image that unit can transfer this bacterium colony to be identified from local storer.
After above-mentioned first acquisition module S1 gets the image of bacterium colony to be identified, it is possible to extract colony characteristics information by said extracted module S2 from the image of bacterium colony to be identified, specifically can be extracted by following extraction unit and generation unit.
Said extracted module S2 comprises extraction unit and generates unit;
Extraction unit, for extracting size, color, shape, the glossiness of bacterium colony to be identified and do wet degree from the image of bacterium colony to be identified;
Generate unit, generate the colony characteristics information of bacterium colony to be identified for the size according to bacterium colony to be identified, color, shape, glossiness and dry wet degree.
Said extracted unit extracts the size of bacterium colony to be identified, first the position of bacterium colony to be identified in the image of this bacterium colony to be identified is needed to locate, then determine, according to the position of this bacterium colony to be identified, the region that bacterium colony to be identified is shared in the picture, and calculate the area in this region, the size of bacterium colony to be identified is determined by the area calculated.
Said extracted unit extracts the color of bacterium colony to be identified, first the region that bacterium colony to be identified is shared in the picture is determined, then the pixel value of each pixel in this region is counted, and pixel value is converted to corresponding numerical value, the probability of the last numerical value corresponding according to each pixel, color comparison card and each pixel distribution, it is determined that go out the color of bacterium colony to be identified. Wherein, color comparison card comprises the one-to-one relationship of each color and numerical value.
Said extracted unit extracts the shape of bacterium colony to be identified, first determines the region that bacterium colony to be identified is shared in the picture, then to be measured the boundary line in this region, determine, according to measuring result and shape computational algorithm, the shape that this bacterium colony to be identified is corresponding.
Said extracted unit extracts the glossiness of bacterium colony to be identified, first the region that bacterium colony to be identified is shared in the picture is determined, then contrast gradient and the bright-dark degree of image in this region is calculated, finally according to the contrast gradient calculated, bright-dark degree and image gloss judging criterion, it is determined that go out the glossiness of bacterium colony to be identified.
Said extracted unit extracts the dry wet degree of bacterium colony to be identified, first the region that bacterium colony to be identified is shared in the picture is determined, then the reflectance of image in this region is calculated, according to the reflectance calculated and dry wet degree synopsis, it is determined that go out the dry wet degree of this bacterium colony to be identified; Wherein, dry wet degree synopsis comprises reflective to the one-to-one relationship with dry wet degree.
In embodiments of the present invention, said extracted unit extracts size, color, shape, the glossiness of bacterium colony to be identified and does the characteristics of image such as wet degree, by in conventional images process technology, the image feature extraction techniques such as the size of image, color, shape, glossiness and dry wet degree can also be realized.
After said extracted unit extracts the size of bacterium colony to be identified, color, shape, glossiness and dry wet degree, the size of bacterium colony to be identified, color, shape, glossiness and dry wet degree are formed the colony characteristics of bacterium colony to be identified by above-mentioned generation module, and the bacterial classification of this bacterium colony to be identified is determined according to the colony characteristics of bacterium colony to be identified, finally using colony characteristics information as bacterium colony to be identified of the colony characteristics of this bacterium colony to be identified and bacterial classification.
After said extracted module S2 extracts the colony characteristics information of bacterium colony to be identified, determine, by above-mentioned determination module S3, the bacterial classification information that bacterium colony to be identified is corresponding. Wherein, above-mentioned determination module S3 determines by following first determining unit and the 2nd determining unit.
Above-mentioned determination module comprises the first determining unit and the 2nd determining unit;
First determining unit, for from the colony characteristics storehouse set up in advance, it is determined that go out the colony characteristics that the colony characteristics information matches degree with bacterium colony to be identified is the highest;
2nd determining unit, for by bacterial classification information corresponding for colony characteristics the highest for matching degree, it is determined that is the bacterial classification information that bacterium colony to be identified is corresponding.
Above-mentioned first determining unit is when determining that the colony characteristics information matches with bacterium colony to be identified is spent, first need to compare each colony characteristics in the colony characteristics information of bacterium colony to be identified and this colony characteristics storehouse, wherein, by each feature in the colony characteristics information of bacterium colony to be identified and in colony characteristics storehouse each feature in each colony characteristics compare, comprise the bacterium colony size in the size of comparison bacterium colony to be identified and each colony characteristics; Colony colour in the color of comparison bacterium colony to be identified and each colony characteristics; Colony shape in the shape of comparison bacterium colony to be identified and each colony characteristics; Bacterium colony glossiness in the glossiness of comparison bacterium colony to be identified and each colony characteristics; And the dry wet degree of the dry bacterium colony wet in degree and each colony characteristics of comparison bacterium colony to be identified. Then, the first determining unit, adds up the quantity of the characteristic item that is consistent of this colony characteristics according to the comparison result of the various features in each colony characteristics in colony characteristics storehouse. Wherein, the data that comparison and statistics obtain are schematically as shown in table 1 below.
Table 1
Finally, the first determining unit, by colony characteristicses maximum for the characteristic item that is consistent that counts, it is determined that be the colony characteristics the highest with the matching degree of the colony characteristics of bacterium colony to be identified.
After the first determining unit determines the colony characteristics the highest with the colony characteristics matching degree of bacterium colony to be identified, it is possible to by above-mentioned 2nd determining unit, by bacterial classification information corresponding for colony characteristics the highest for matching degree, it is determined that be the bacterial classification information that bacterium colony to be identified is corresponding.
In embodiments of the present invention, first determining unit is when determining that the colony characteristics information matches with bacterium colony to be identified is spent, if when in colony characteristics storehouse, the matching degree of the colony characteristics of all colony characteristicses and bacterium colony to be identified is all lower than default matching degree, now the 2nd determining unit cannot accurately determine the bacterial classification of bacterium colony to be identified according to the colony characteristics in colony characteristics storehouse, also need the concise and to the point Biochemical Information supplementing the corresponding bacterium of qualification bacterium colony to be identified in conjunction with corresponding authenticate technology, the concise and to the point Biochemical Information of the corresponding bacterium of the bacterium colony to be identified according to supplementary qualification determines the bacterial classification of bacterium colony to be identified further, after the 2nd determining unit finally determines the bacterial classification of this bacterium colony to be identified, can according to the bacterial classification determined, the concise and to the point Biochemical Information supplementing the corresponding bacterium of bacterium colony to be identified of qualification generates the bacterial classification information of this bacterium colony to be identified, and this bacterial classification information can be added to colony characteristics storehouse, for the qualification of follow-up bacterium colony to be identified provides appraisal basis, thus the determination rates of bacterium colony can also be improved.
In embodiments of the present invention, can also stored in a large amount of Colony hybridization in above-mentioned colony characteristics storehouse, the corresponding unique bacterial classification information of each Colony hybridization.When it needs to be determined that during the bacterial classification information of bacterium colony to be identified, first, above-mentioned first acquisition module S1 obtains the image of bacterium colony to be identified, and then, said extracted module S2 extracts colony characteristics information from the image of bacterium colony to be identified; Finally, above-mentioned determination module S3 is according to Colony hybridization in the colony characteristics information extracted and colony characteristics storehouse, it is determined that the bacterial classification information that bacterium colony to be identified is corresponding. Wherein, first acquisition module S1 obtains the image of bacterium colony to be identified, can being obtained by the Colony hybridization acquisition methods of above-mentioned offer, extraction module S2 extracts colony characteristics information from the image of bacterium colony to be identified, it is possible to extracted by the colony characteristics extracting method of above-mentioned offer.
Above-mentioned determination module S3 is according to the Colony hybridization in the colony characteristics information extracted and colony characteristics storehouse, it is determined that the bacterial classification information that bacterium colony to be identified is corresponding, it is possible to realized by following process.
From colony characteristics storehouse in Colony hybridization, it is determined that go out the Colony hybridization that the colony characteristics information matches degree with bacterium colony to be identified is the highest; By bacterial classification information corresponding for Colony hybridization the highest for matching degree, it is determined that be the bacterial classification information that described bacterium colony to be identified is corresponding.
Above-mentioned bacterial classification information bacterium colony kind and colony characteristics corresponding to bacterium colony kind. determination module S3 is when determining the highest Colony hybridization of the colony characteristics information matches degree with bacterium colony to be identified, first the colony characteristics being extracted all Colony hybridization in colony characteristics storehouse by said extracted module S2 is needed, then the colony characteristics of the colony characteristics information of bacterium colony to be identified and each Colony hybridization is compared, wherein, each feature in the colony characteristics of each feature in the colony characteristics information of bacterium colony to be identified and each Colony hybridization is compared, comprise the bacterium colony size in the size of comparison bacterium colony to be identified and the colony characteristics of each Colony hybridization, colony colour in the color of comparison bacterium colony to be identified and the colony characteristics of each Colony hybridization, colony shape in the shape of comparison bacterium colony to be identified and the colony characteristics of each Colony hybridization, bacterium colony glossiness in the glossiness of comparison bacterium colony to be identified and the colony characteristics of each Colony hybridization, and the dry wet degree of the bacterium colony in the colony characteristics of the dry wet degree of comparison bacterium colony to be identified and each Colony hybridization. then, the quantity of the characteristic item that is consistent of this colony characteristics is added up according to the comparison result of the various features in the colony characteristics of each Colony hybridization. wherein, the data that comparison and statistics obtain are schematically as shown in Table 1.
Finally, it is determined that module S3 is by colony characteristicses maximum for the characteristic item that is consistent that counts, it is determined that be the colony characteristics the highest with the matching degree of the colony characteristics of bacterium colony to be identified.
After determination module S3 determines the colony characteristics the highest with the colony characteristics matching degree of bacterium colony to be identified, determination module S3 is according to the corresponding relation of Colony hybridization and bacterial classification information, by bacterial classification information corresponding for the Colony hybridization comprising the highest colony characteristics of matching degree, it is determined that be the bacterial classification information that bacterium colony to be identified is corresponding.
In embodiments of the present invention, when identifying the bacterial classification information of bacterium colony to be identified, only need to obtaining the image of bacterium colony to be identified, appraiser does not need actual contact bacterium colony to be identified, avoid and the bacteriological infection that bacterium colony to be identified contacts and cause, thus improve the security of appraiser; In whole qualification process, appraiser does not need to be grasped very professional bacterium and medical knowledge, it is only necessary to grasp the operation of relevant equipment and related software, and the professional requirement of appraiser is very low; The bacterial classification information of bacterium colony is identified by method provided by the invention, the image of bacterium colony to be identified can be got fast, and complete qualification process fast by the information processing device of automatization and software, therefore qualification result can be obtained fast, substantially increase determination rates, and qualification result accuracy is very high; In the present invention, the image of bacterium colony to be identified can be obtained in several ways, whole qualification process does not need the expense that bacterium colony to be identified carries out the payments such as preservation, transport and management, the required expense paid of appraiser is also very low, the cost of Bacteria Identification device is very low, thus greatly reduces appraisal cost.
In embodiments of the present invention, Bacteria Identification device comprises the first acquisition module, for obtaining the image of bacterium colony to be identified;Extraction module, for extracting colony characteristics information from the image of bacterium colony to be identified; Determination module, for according to the colony characteristics information extracted and the colony characteristics storehouse set up in advance, it is determined that the bacterial classification information that bacterium colony to be identified is corresponding. Bacterium colony carries out strain identification by the mode by noncontact formula that achieves, it is to increase the security of appraiser; This method for determining bacteria and device are very low to the professional requirement of the bacterium of appraiser and medical treatment aspect; Qualification process can be completed fast by the information process of automatization, and obtain qualification result fast, substantially increase determination rates, and qualification result accuracy is very high; Can obtaining the image of bacterium colony to be identified in several ways, Bacteria Identification device expense is very low, thus greatly reduces appraisal cost.
The above; it is only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, any it is familiar with those skilled in the art in the technical scope that the present invention discloses; change can be expected easily or replace, all should be encompassed within protection scope of the present invention. Therefore, protection scope of the present invention should be as the criterion with the protection domain of described claim.

Claims (10)

1. method for determining bacteria, it is characterised in that, described method comprises:
Obtain the image of bacterium colony to be identified;
Colony characteristics information is extracted from the image of described bacterium colony to be identified;
According to the described colony characteristics information extracted and the colony characteristics storehouse set up in advance, it is determined that the bacterial classification information that described bacterium colony to be identified is corresponding.
2. method according to claim 1, it is characterised in that, described from the image of described bacterium colony to be identified, extract colony characteristics information, comprising:
From the image of described bacterium colony to be identified, extract size, color, shape, the glossiness of described bacterium colony to be identified and do wet degree;
Size according to described bacterium colony to be identified, color, shape, glossiness and dry wet degree generate the colony characteristics information of described bacterium colony to be identified.
3. method according to claim 1, it is characterised in that, described according to the described colony characteristics information extracted and the colony characteristics storehouse set up in advance, it is determined that the bacterial classification information that described bacterium colony to be identified is corresponding, comprising:
From the described colony characteristics storehouse set up in advance, it is determined that go out the colony characteristics the highest with the colony characteristics information matches degree of described bacterium colony to be identified;
By bacterial classification information corresponding for colony characteristics the highest for described matching degree, it is determined that be the bacterial classification information that described bacterium colony to be identified is corresponding.
4. method according to claim 1, it is characterised in that, the image of described acquisition bacterium colony to be identified, comprising:
The bacterium colony picture collection device carried by terminal gathers the image of bacterium colony to be identified, or the image of the bacterium colony to be identified of the device transmission received except described terminal, or transfers the image of the bacterium colony to be identified of storage from this locality.
5. method according to claim 1, it is characterised in that, described method also comprises:
The colony characteristics that the bacterial classification information that acquisition various species bacterium colony is corresponding, described bacterial classification information bacterium colony kind and described bacterium colony kind are corresponding;
The bacterial classification information corresponding according to described various species bacterium colony sets up colony characteristics storehouse.
6. Bacteria Identification device, it is characterised in that, described device comprises:
First acquisition module, for obtaining the image of bacterium colony to be identified;
Extraction module, for extracting colony characteristics information from the image of described bacterium colony to be identified;
Determination module, for according to the described colony characteristics information extracted and the colony characteristics storehouse set up in advance, it is determined that the bacterial classification information that described bacterium colony to be identified is corresponding.
7. device according to claim 6, it is characterised in that, described extraction module comprises:
Extraction unit, for extracting size, color, shape, the glossiness of described bacterium colony to be identified and do wet degree from the image of described bacterium colony to be identified;
Generate unit, generate the colony characteristics information of described bacterium colony to be identified for the size according to described bacterium colony to be identified, color, shape, glossiness and dry wet degree.
8. device according to claim 6, it is characterised in that, described determination module comprises:
First determining unit, for from the described colony characteristics storehouse set up in advance, it is determined that go out the colony characteristics the highest with the colony characteristics information matches degree of described bacterium colony to be identified;
2nd determining unit, for by bacterial classification information corresponding for colony characteristics the highest for described matching degree, it is determined that is the bacterial classification information that described bacterium colony to be identified is corresponding.
9. device according to claim 6, it is characterised in that, described first acquisition module comprises:
Collecting unit, the bacterium colony picture collection device for being carried by terminal gathers the image of bacterium colony to be identified;
Receive unit, for the image of the bacterium colony to be identified of device transmission received except described terminal;
Transfer unit, for transferring the image of the bacterium colony to be identified of storage from this locality.
10. device according to claim 6, it is characterised in that, described device also comprises:
2nd acquisition module, for obtaining bacterial classification information corresponding to various species bacterium colony, described bacterial classification information bacterium colony kind and colony characteristics corresponding to described bacterium colony kind;
Set up module, set up colony characteristics storehouse for the bacterial classification information corresponding according to described various species bacterium colony.
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