CN105671057A - Application of gene OsTPPC in regulating total phosphorus and phytic acid levels in rice kernels - Google Patents
Application of gene OsTPPC in regulating total phosphorus and phytic acid levels in rice kernels Download PDFInfo
- Publication number
- CN105671057A CN105671057A CN201610157873.2A CN201610157873A CN105671057A CN 105671057 A CN105671057 A CN 105671057A CN 201610157873 A CN201610157873 A CN 201610157873A CN 105671057 A CN105671057 A CN 105671057A
- Authority
- CN
- China
- Prior art keywords
- gene
- ostppc
- rice
- total phosphorus
- phytic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses application of gene OsTPPC in regulating total phosphorus and phytic acid levels in rice kernels. A nucleotide sequence of the gene OsTPPC is shown as in SEQ ID No. 1. By using low total phosphorus and low phytic acid kernel phenotypes of two rice mutants and cloning by means of map-based cloning, the gene OsTPPC for regulating total phosphorus and phytic acid levels of rice kernels is obtained, and the action of the gene OsTPPC in regulating the total phosphorus and phytic acid levels in rice kernels is verified through techniques such as genetic complementary transformation and RNA interference.
Description
Technical field
The present invention relates to field of plant genetic, particularly relate to the application in adjusting and controlling rice seed total phosphorus and phytic acid content of a kind of OsTPPC gene.
Background technology
Oryza sativa L. is one of topmost cereal crops in the world, particularly there are about the population of more than half in Asia with rice for staple food. And the micronutrient element deficiency diseases such as rice country of consumption resident ubiquity ferrum, zinc, especially China is the major country that world rice produces and consumes, and is again that one of more serious country occurs for iron deficiency and iron deficiency anemia in the world.
Phosphorus (P) element in rice mainly exists with the form of phytate, due to people and the internal phytase gene without degraded phytate of nonruminant (chicken, pig, fish etc.), not only result in the phytate in food cannot be absorbed by the body utilization, simultaneously phytate enter after human body also can further with the metal micronutrient element complexation such as the ferrum in other sources, zinc, reduce its biological effectiveness.
In addition, the rice bran comprising silverskin, aleurone and rice germ is important animal feed, but nonruminant is the same with people cannot utilize Physic Acid in Foods salt (phosphorus element and metal trace element), and also because contained phosphorus content exceedes needs after other class animal edible, these unavailable phosphorus elements are finally discharged in environment and go to cause severe contamination. Therefore, always strive in the world cultivate total phosphorus and all significantly reduced variety of crops of phytic acid content, to improve its nutritive value and environmental friendliness performance (RaboyV.2009.Approachesandchallengestoengineeringseedphyt ateandtotalphosphorus.PlantScience177:281 296).
In Fructus Hordei Vulgaris, it is found that mutant (the RaboyV of low total phosphorus and low phytic acid so far, CichyK, PetersonK, ReichmanS, SompongU, SrinivesP, SaneokaH.2014.Barley (HordeumvulgareL.) LowPhyticAcid1-1:anendosperm-specific, filialdeterminantofseedtotalphosphorus.JournalofHeredity 105:656 665.), but not yet find to control the gene of this " double; two low " characteristic so far in plant, thus also but without a kind of reliable method producing double; two low seed.
Summary of the invention
The invention provides the application in adjusting and controlling rice seed total phosphorus and phytic acid content of a kind of OsTPPC gene, in this OsTPPC gene, the disappearance of base can reduce the content of total phosphorus in rice grain and the content of phytic acid, for the rice varieties of the low total phosphorus of selection-breeding and low phytic acid.
Present invention finds the application in adjusting and controlling rice seed total phosphorus content of the OsTPPC gene, the nucleotide sequence of described OsTPPC gene is such as shown in SEQIDNO.1.
It has also been found that OsTPPC gene application in adjusting and controlling rice seed phytic acid content, the nucleotide sequence of described OsTPPC gene is such as shown in SEQIDNO.1.
The aminoacid sequence of the above-mentioned OsTPPC gene protein that number is KT188441, OsTPPC gene code in GenBank is such as shown in SEQIDNO.2, and the number in GenBank is KT188445; The sequence of OsTPPC gene coding region is such as shown in SEQIDNO.3.
Present invention also offers the breeding method of a kind of low total phosphorus and phytic acid rice, including:
(1) the RNAi hairpin structure unit of OsTPPC gene is built;
(2) described hairpin structure unit is connected into intermediate carrier, builds plant expression vector;
(3) by described plant expression vector by agrobacterium mediation converted Rice Callus;
(4) being transferred to by Rice Callus and continue on selective medium to cultivate, treat seedling differentiation, transplant land for growing field crops, screening obtains low total phosphorus and phytic acid rice plant;
The nucleotide sequence of described OsTPPC gene is such as shown in SEQIDNO.1.
RNAi hairpin structure unit mentioned above refers to the hairpin structure of middle one " genes of interest fragment-intron-genes of interest fragment (the reversely) " form formed for the genes of interest fragment reverse complemental of intron, two ends; Wherein genes of interest fragment is the CDS sequence of OsTPPC gene, and sequence is such as shown in SEQIDNO.3; And intron is without strict demand, in the present invention, intron is from pBSSK-IN carrier, and base sequence is such as shown in SEQIDNO.8.
Specifically, described plant expression vector is pCAMBIA1301-35SN. Described Agrobacterium is EHA105. The kind of described Oryza sativa L. is that Japan is fine.
Present invention also offers the breeding method of a kind of low total phosphorus and phytic acid rice, including:
(1) with the rice plant containing OsTPPC gene for female parent, mutant MH86-lpa is male parent, hybridizes, it is thus achieved that F1Generation;
(2)F1After selfing, it is thus achieved that F2Generation;
(3) to F2Pcr gene detection is carried out, from F for seedling2In generation, picks out the individual plant only with 178bp characteristic strip, it is thus achieved that the Oryza sativa L. of low total phosphorus and low phytic acid.
Wherein, described female parent is Jiahe 218.
The present invention is by the seed phenotype of two low total phosphorus of rice mutant, low phytic acid, map-based cloning clone is adopted to obtain the OsTPPC gene of adjusting and controlling rice seed total phosphorus and phytic acid content, and by technology such as genetic complementation conversion, RNA interference, demonstrate the effect in adjusting and controlling rice seed total phosphorus and phytic acid content of the OsTPPC gene.
Accompanying drawing explanation
Fig. 1 is the gene mapping and the clone figure that control the low total phosphorus of MH86-lpa and Z9B-lpa, low phytic acid characteristic;
A () MH86-lpa mutant gene Primary Location is at Rice Chromosome 4, chain with SSR molecular marker RM5478 and RM17567;
B () MH86-lpa mutant gene is finely positioned in the 112kb region between molecular marker ID2 and C1;
14 predicted genes are comprised in (c) candidate region;
(d) OsTPPC gene structure and mutational site schematic diagram, wherein there is a base deletion (+1828) at the 12nd exon of LOC_Os04g55800 in MH86-lpa; Z9B-lpa is 6 bases (+297-303) of the 1st Exon deletion;
(e) OsTPPC protein structure domain and mutation effect thereof, wherein in MH86-lpa, codon translation terminates in advance, produces the albumen of a truncate; Z9B-lpa then lacks 2 aminoacid;
Fig. 2 is the phytate phosphorus chromatography of ions detection peak figure of OsTPPC functional complementation material;
(a) MH86 wild type;(b) MH86-lpa mutant; (c) MH86-lpa complementation transfer-gen plant; The incomplementarity sister system of (d) MH86-lpa transgenic complementary materials.
Fig. 3 is embodiment 3 Middle molecule marker assisted selection electrophoretogram, it may be assumed that MH86-lpa, Jiahe 218 and their F2Separate the gel electrophoresis gene type figure of offspring;
M:DNA molecular weight standard; 1,2 and 3 wild type, mutant and hybridization F are represented respectively1For plant sample; 4-18 is F2Hybridization separates offspring.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is described further.
Molecular biology and biochemical method that following example use are known technology, and experiment material used in following example is commercially available purchase product if no special instructions.
The discovery of embodiment 1OsTPPC gene function
Rice mutant MH86-lpa and Z9B-lpa is obtained (LiuQL by bright extensive 86 (MH86) of rice variety and " middle 9B " by gamma-ray and mutagenesis respectively, XuXH, RenXL, FuHW, WuDX, ShuQY.2007.Generationandcharacterizationoflowphyticacidg ermplasminrice (oryzasativaL.) .TheoreticalandAppiedlGenetics114:803-814).
Through experiment, we have found that this two parts of rice mutant MH86-lpa and Z9B-lpa, plant when multiple years varying environment, total phosphorus and phytic acid content all pole in they seeds are substantially less than bright extensive 86 (MH86) of its corresponding wild-type parent and middle 9B (Z9B), namely showing as the seed phenotype of low total phosphorus, low phytic acid, result is as shown in table 1.
Total phosphorus in table 1 rice mutant and wild-type parent brown rice thereof and phytate phosphorus content
1Numerical value is that mean+SD (n=3) .** and * represents that its corresponding wild type of value of mutant exists extremely notable (P < 0.01) and significant difference (P < 0.05) respectively.
Adopting map-based cloning to identify the gene controlling the low total phosphorus of above-mentioned Oryza sativa L., low phytic acid, identified, its gene number in Oryza sativa L. functional genome is LOC_Os04g55800, and annotation is a gene OsTPPC possible coding sulfate transport gene family 3. The particular content of above-mentioned qualification process is as follows:
MH86-lpa material is fine with normal phenotype japonica rice variety Japan hybridizes, F1For selfing, obtain F2Colony, at each individual plant labelling of tillering regularity and take the tender leaf of about 1 gram, then individual plant results F2:3Seed; Adopt the phosphorus coherent element content (ZhaoHJ of the method detection rice paddy seeds such as Zhao, CuiHR, XuXH, TanYY, FuJJ, LiuGZ, PoirierY, ShuQY.2013.Characterizationofosmikinaricemutantwithreduc edphytatecontentrevealsaninsertionofarearrangedretrotran sposon.TheoreticalandAppiedlGenetics126:3009-3020).
Therefrom select 1181 strains and there is the low total phosphorus individuality with low phytic acid mutation phenotype as locator material, find out the numbering blade of correspondence, adopt conventional CTAB method to extract STb gene; BSA method is adopted to combine between the 350 pairs of Oryza sativa L. SSR molecular marker Primary Location announced RM5478 and RM17567 molecular marker on Chromosome 4 in Rice is long-armed (Fig. 1 a).
According to the fine Sequencing of Rice Genome information with long-grained nonglutinous rice 9311 of japonica rice Japan announced, we have designed and developed new molecular marker further, OsTPPC gene is accurately located at No. BAC within the scope of 112kb on AL606690 section the most at last, the molecular marker on both sides respectively ID2 and C2, and be divided into from (Fig. 1 b) with molecular marker C1.
To the gene of 14 Bioinformatics Predictions in this region, amplification order-checking comparison analysis one by one respectively in MH86-lpa mutant and wild type MH86, allelic variant is there is in MH86-lpa and Z9B-lpa in gene LOC_Os04g55800, there is the disappearance of a 1bp in the former, cause terminator codon in advance occur and then cause that encoding proteins shortens on LOC_Os04g55800; There is the disappearance of a 6bp in the latter, cause its coding 2 aminoacid of protein delation (Fig. 1 d, e).
Embodiment 2OsTPPC functional complementation is tested
For determining that low total phosphorus in above-mentioned rice grain, low phytic acid characteristic disappearance is caused by LOC_Os04g55800 sudden change, carry out transgenic complementary assay.
Particular content is as follows:
Rice genome BAC is cloned AL606690, with restricted enzyme KpnI and EcoRI complete degestion, after electrophoretic separation, purification reclaims the DNA fragmentation of about 12kb, and this fragment is connected in expression vector pCAMBIA1301, this clone covers the genome area of this ORF, including the regulating and controlling sequence (also comprising the nucleotide sequence shown in SEQIDNO.1) of start codon ATG upstream 3.96kb promoter sequence and the 2.6kb in termination codon TGA downstream, thus being built into binary plasmid carrier pCAMBIA1301+promoter+OsTPPC+nos.
This plasmid is proceeded to rice transformation MH86-lpa in Agrobacterium EHA105 by the method for thermal shock.
Idiographic flow is as follows: utilize MH86-lpa mature embryo-derived callus, through inducing culture subculture 2-3 generation, select growth vigorous, color cadmium yellow, the close embryo callus granule of quality does the receptor converted, rice callus is contaminated with the Agrobacterium EHA105 bacterial strain containing above-mentioned binary plasmid carrier, 25 DEG C of light culture are after 3 days, screening culture medium containing 30mg/LHygromycin is cultivated 14 days, repeat screening and culturing 1 time, then the callus filtered out is gone to and the screening culture medium containing 50mg/LHygromycin continues screening 14 days, the kanamycin-resistant callus tissue continuing merisis is turned and illumination cultivation on division culture medium, after about 7 days, wound healing starts to turn green, start to put out new shoots after 21 days, then move to and root media grows 14 days, land for growing field crops is proceeded to after seedling exercising.
The analysis of complementary transfer-gen plant and incomplementarity sister's system's seed total phosphorus and phytic acid content is confirmed; the low total phosphorus of MH86-lpa, low phytic acid phenotype are caused by LOC_Os04g55800 sudden change, and namely complementary successfully MH86-lpa transgenic paddy rice seed has recovered normal total phosphorus and phytate phosphorus content (as shown in table 2).
Table 2 Oryza sativa L. MH86-lpa transgenic is complementary and total phosphorus in control material brown rice and phytate phosphorus content
1MH86-lpaCL1, MH86-lpaCL2 are MH86-lpa complementary wild-type LOC_Os04g55800 genetic material; MH86-lpa-NCS1 and MH86-lpaNCS2 is its incomplementarity sister system.2Numerical value is that mean+SD (n=3) .** and * represents that the corresponding incomplementarity material of the value of complementary materials exists extremely notable (P < 0.01) and significant difference (P < 0.05).
The application in paddy rice cross breeding breeding of the embodiment 3OsTPPC gene
The present embodiment adopt molecular marker assisted selection means, it is thus achieved that low total phosphorus, low phytic acid rice new strain.
Jiahe 218 is normal water rice varieties, does not namely have the character of low total phosphorus and low phytic acid, so that this kind to carry out low total phosphorus and low phytic acid improvement, illustrates.
Particular content is as follows:
With Jiahe 218 for maternal, the MH86-lpa containing OsTPPC gene is that male parent carries out hybridization acquisition F1, after selfing, obtain F2Colony.
Adopting dCAPSFinder2.0 software design dCAPS molecular marker according to MH86-lpaOsTPPC gene 1bp disappearance, its primer sequence is as follows:
Forward primer sequence: ZH1F is 5 '-CGACTTGAAGAAATCAACAGAGAAGCCTG-3 ';
Reverse primer sequences: ZH1R is 5 '-GGGGTTTACTATGCCACATAACT-3 '.
Containing the recognition site of restricted enzyme ECORII in the 179bpPCR amplified production in Jiahe 218, can be digested for 152bp, and without ECORII recognition site in the 178bp amplified production of MH86-lpa, therefore can not enzyme action. The difference that the two fragment is shown in can be easy to carry out effectively differentiation (as shown in Figure 2) by the polyacrylamide gel electrophoresis of 10.0%.
Utilize design synthetic primer ZH1, the F to the homozygous material MH86-lpa of the gene mutation containing OsTPPC and the homozygous material Jiahe 218 without OsTPPC gene mutation2Filial generation carries out genotype detection in seedling stage, and result as in figure 2 it is shown, be divided into 3 kinds of genotype by material: has the material of 178bp characteristic strip, has the material that the material of 152bp characteristic strip has concurrently with both.
Results F2F on plant2:3Seed, analyze total phosphorus and the phytate phosphorus content of each individual plant, it is shown that the individual plant of all 178bp of having characteristic strips is all low total phosphorus and low phytic acid individual plant, and there is 152bp characteristic strip and be total phosphorus and the normal individual plant of phytic acid content (table 2) with the two material having concurrently. Prove can the low total phosphorus of OsTPPC Gene Handling and low phytic acid character stably be transferred in other rice materials by cross-breeding means.
The genotype of table 2OsTPPC gene recombination offspring's individual plant is identified with Phenotype
Embodiment 4OsTPPC gene silencing reduces rice paddy seed phytic acid content
After clone's OsTPPC gene, it is possible to adopt multiple hereditism's means that composition and the expression of this gene are operated so that it is produce sudden change, or make its gene expression dose decline, thus cultivating low total phosphorus, phytic acid rice new varieties.
These genetic manipulation means include but are not limited to brings out process and mutant gene orthoselection (TILLING technology) (KimSI, TaiTH.2014.Identificationofnovelricelowphyticacidmutatio nsviaTILLINGbysequencing.MolecularBreeding34:1717-1729), and such as the directed mutagenesis techniques (ZhangH of CRISPR/Cas9 mediation, ZhangJ, WeiP, ZhangB, GouF, FengZ, MaoY, YangL, ZhangH, XuN, ZhuJK.2014.TheCRISPR/Cas9systemproducesspecificandhomozy goustargetedgeneeditinginriceinonegeneration.PlantBiotec hnolJ12:797-807.), and adopt the RNA perturbation technique (LiWX such as artificial Microrna and hairpin RNA, HuangJZ, ZhaoHJ, TanYY, CuiHR, PoirierY, ShuQY.2014.ProductionoflowphyticacidricebyhairpinRNAanda rtificialmicrorna-mediatedsilencingofOsMIKinseeds.PlantC ell, TissueandOrganCulture (PCTOC) 119:15-25) suppress the expression of OsTPPC, thus cultivating reduction rice grain total phosphorus and phytate phosphorus content.
The present embodiment hinders and damage the rice Os TPPC gene function low total phosphorus of acquisition for RNA and low phytic acid material illustrates.
Extracting the total serum IgE in bright extensive 86 seeds of Oryza sativa L., reverse transcription becomes cDNA, designs the Partial Fragment of primer amplification OsTPPC gene coding region according to sequence SEQIDNO.3, and primer information is as follows:
ZH2F:5 '-CAGTTGGTAGGACATTTGCTTCA-3 ';
ZH2R:5’-TGCCTTTGATGTTCCCTTGA-3’。
Pcr amplification product is after cloning and sequencing is verified, the RNAi hairpin structure of " genes of interest fragment, intron, genes of interest fragment (reversely) " is formed respectively through the pBSSK-INRNAi intermediate carrier being connected into same process after restricted enzyme NotI+BamHI and KpnI+XhoI enzyme action, finally, it is connected into expression vector pCAMBIA1301-35SN (being obtained by pCAMBIA1301 vector modification) by NotI+KpnI double enzyme site. Identifying that the positive clone obtained imports in Agrobacterium EHA105, rice transformation japonica rice variety Japan is fine. Transgenic procedures is with reference to embodiment 2.
In 12 the transgenic independence strains obtained, after after GUS colour developing and gene expression molecule, RT-PCR analyzes, it is thus achieved that 8 positive T1Plant, continues field plot test and obtains 5 stable progeny transgenic strains. P elements analysis shows: compares total phosphorus and phytate phosphorus content in comparison, RNA interfering material brown rice and all declines to a great extent, respectively up to 28%, 57%, (as shown in table 3).
Total phosphorus in table 3OsTPPC gene silencing brown rice and phytate phosphorus content
1RP1, RP2 are OsTPPC gene RNA interfering material; RN1, RN2 are the incomplementarity sister system of its correspondence.2Numerical value is that mean+SD (n=3) .** and * represents that the corresponding incomplementarity material of the value of complementary materials exists extremely notable (P < 0.01) and significant difference (P < 0.05).
Claims (8)
- The application in adjusting and controlling rice seed total phosphorus content of the 1.OsTPPC gene, the nucleotide sequence of described OsTPPC gene is such as shown in SEQIDNO.1.
- The application in adjusting and controlling rice seed phytic acid content of the 2.OsTPPC gene, the nucleotide sequence of described OsTPPC gene is such as shown in SEQIDNO.1.
- 3. the breeding method of a low total phosphorus and phytic acid rice, it is characterised in that including:(1) the RNAi hairpin structure unit of OsTPPC gene is built;(2) described hairpin structure unit is connected into intermediate carrier, builds plant expression vector;(3) by described plant expression vector by agrobacterium mediation converted Rice Callus;(4) being transferred to by Rice Callus and continue on selective medium to cultivate, treat seedling differentiation, transplant land for growing field crops, screening obtains low total phosphorus and phytic acid rice plant;The nucleotide sequence of described OsTPPC gene is such as shown in SEQIDNO.1.
- 4. breeding method as claimed in claim 3, it is characterised in that described plant expression vector is pCAMBIA1301-35SN.
- 5. breeding method as claimed in claim 3, it is characterised in that described Agrobacterium is EHA105.
- 6. breeding method as claimed in claim 3, it is characterised in that the kind of described Oryza sativa L. is that Japan is fine.
- 7. the breeding method of a low total phosphorus and phytic acid rice, it is characterised in that including:(1) with the rice plant containing OsTPPC gene for female parent, mutant MH86-lpa is male parent, hybridizes, it is thus achieved that F1Generation;(2)F1After selfing, it is thus achieved that F2Generation;(3) to F2Pcr gene detection is carried out, from F for seedling2In generation, picks out the individual plant only with 178bp characteristic strip, it is thus achieved that the Oryza sativa L. of low total phosphorus and low phytic acid.
- 8. breeding method as claimed in claim 7, it is characterised in that described female parent is Jiahe 218.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610157873.2A CN105671057A (en) | 2016-03-18 | 2016-03-18 | Application of gene OsTPPC in regulating total phosphorus and phytic acid levels in rice kernels |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610157873.2A CN105671057A (en) | 2016-03-18 | 2016-03-18 | Application of gene OsTPPC in regulating total phosphorus and phytic acid levels in rice kernels |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105671057A true CN105671057A (en) | 2016-06-15 |
Family
ID=56310999
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610157873.2A Pending CN105671057A (en) | 2016-03-18 | 2016-03-18 | Application of gene OsTPPC in regulating total phosphorus and phytic acid levels in rice kernels |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105671057A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112708629A (en) * | 2021-02-25 | 2021-04-27 | 江西农业大学 | Phytase gene phy1883 from aspergillus niger and application thereof in improving tillering number and effective spike number of rice |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1528124A (en) * | 2003-10-15 | 2004-09-15 | 浙江大学 | Method for breeding corn with low phytic acid content |
| CN1765915A (en) * | 2005-09-26 | 2006-05-03 | 中国农业大学 | High yield rice cultivation method and specific molecular mark |
| CN102864156A (en) * | 2012-09-26 | 2013-01-09 | 浙江大学 | Rice phytic acid related gene ZJU-LPA1 and method for cultivating low-phytic-acid rice |
-
2016
- 2016-03-18 CN CN201610157873.2A patent/CN105671057A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1528124A (en) * | 2003-10-15 | 2004-09-15 | 浙江大学 | Method for breeding corn with low phytic acid content |
| CN1765915A (en) * | 2005-09-26 | 2006-05-03 | 中国农业大学 | High yield rice cultivation method and specific molecular mark |
| CN102864156A (en) * | 2012-09-26 | 2013-01-09 | 浙江大学 | Rice phytic acid related gene ZJU-LPA1 and method for cultivating low-phytic-acid rice |
Non-Patent Citations (2)
| Title |
|---|
| ZHAO,H ET AL.: "Zhao et al.，Oryza sativa Indica Group isolate MH86 sulfate transporter (SULTR3:3) gene, complete cds，GenBank: KT188441.1", 《GENBANK》 * |
| 王玉华等: "水稻高无机磷突变体的筛选和培育技术研究", 《中国水稻科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112708629A (en) * | 2021-02-25 | 2021-04-27 | 江西农业大学 | Phytase gene phy1883 from aspergillus niger and application thereof in improving tillering number and effective spike number of rice |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106998665A (en) | The generation of haplophyte | |
| CN102634522B (en) | Gene for controlling rice fertility, encoded protein and application thereof | |
| CN108291234A (en) | Multiple sporinite forms gene | |
| CN101128591A (en) | Expression cassettes for seed-preferential expression in plants | |
| UA128618C2 (en) | Modified plants with enhanced traits | |
| CN103555711A (en) | Non-transgenic genome directed molecule improvement method and application of main crops | |
| CN109152344A (en) | Genetically modified plants with enhancing character | |
| CN102782141A (en) | Expression cassettes for embryo-specific expression in plants | |
| AU2012234910A1 (en) | Stress tolerant wheat plants | |
| CN109477091A (en) | Construct and carrier for the conversion of gene implants | |
| CN102477092B (en) | Protein used for controlling anthocyanidin content, coding gene thereof, and application thereof | |
| CN114946639B (en) | Application of two wheat induction lines in identification of wheat haploid embryo and grain | |
| CN103820480B (en) | The application of a kind of rice transcription factor in improvement rice high yield proterties | |
| CN105671057A (en) | Application of gene OsTPPC in regulating total phosphorus and phytic acid levels in rice kernels | |
| CA2300681C (en) | Tolerance of trichothecene mycotoxins in plants and animals through the modification of the ribosomal protein l3 gene | |
| Wang et al. | Transformation of LRP gene into Brassica napus mediated by Agrobacterium tumefaciens to enhance lysine content in seeds | |
| CN108409844A (en) | Applications of the protein TaNRT2.5 in regulating and controlling plant products | |
| JP5190928B2 (en) | Phospholipase D-deficient rice line | |
| CN102321633B (en) | Pleiotropic gene for controlling vegetative growth and development of floral organs of rice and application thereof | |
| CN110891416B (en) | Genetic basis of Pythium resistance | |
| CN102206652B (en) | Rice gel consistency control gene GC6 and application thereof | |
| CN105969796A (en) | Method creating rice high-yield material by utilizing TALENs (transcription activator-like effector nucleases) technology for site-directed mutagenesis of gene GW2 | |
| CN116041466B (en) | Barley grain black character HvBlp gene and related molecular marker and application thereof | |
| CN104837335A (en) | Wheat with new alleles of Rht-b1 | |
| CN104450739B (en) | A kind of paddy rice source anti insect related gene OsHR1 and coded product thereof and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160615 |
|
| RJ01 | Rejection of invention patent application after publication |