CN105664862A - Porphyridium extracellular polysaccharide adsorbent and preparation method thereof - Google Patents
Porphyridium extracellular polysaccharide adsorbent and preparation method thereof Download PDFInfo
- Publication number
- CN105664862A CN105664862A CN201410664269.XA CN201410664269A CN105664862A CN 105664862 A CN105664862 A CN 105664862A CN 201410664269 A CN201410664269 A CN 201410664269A CN 105664862 A CN105664862 A CN 105664862A
- Authority
- CN
- China
- Prior art keywords
- extracellular polysaccharide
- porphyridium cruentum
- obtains
- porphyridium
- adsorbent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to a porphyridium extracellular polysaccharide adsorbent and a preparation method thereof. The method comprises the steps of porphyridium extracellular polysaccharide separation purification treatment, porphyridium extracellular polysaccharide sulfonation treatment and porphyridium extracellular polysaccharide immobilization treatment. The adsorbent prepared with the method can selectively adsorb heavy metal ions and precious metal ions in water body. The porphyridium extracellular polysaccharide adsorbent has the advantages of fast reaction, high adsorbent capacity, high removal rate, and easy elution. The adsorbent can be repeatedly used, such that secondary pollution to the environment can be reduced. Therefore, the adsorbent is environment-friendly. With the adsorbent, ecological risks caused by pollution residue and species introduction are reduced. The adsorbent and the method are suitable for industrialized productions.
Description
Technical field
The present invention relates to biochemical absorbent fields, the preparation method relating to a kind of Porphyridium cruentum extracellular polysaccharide adsorbent particularly.
Background technology
Along with social development and technological progress, new requirement is proposed: adsorbance wants big, adsorption rate is fast, selects absorbability big, the desired substance in solution or harmful substance can be adsorbed selectively and be separated to separating material, desorption is easy, energy Reusability; Low price, durable in use, wide material sources; Can use in a variety of forms; Isolation technics is simple, less energy consumption, wide in variety, can require to use different cultivars according to difference.
In view of requirements above, biological adsorption material enters the visual field of people with its exclusive advantage, and domestic and international many scholars have carried out extensive and deep research around biological adsorption agent. Biological adsorption agent is a kind of special ion-exchanger, and different from conventional ion-exchanger, what work is biological cell, also mainly has thalline, algae and cell extract etc.
The loose structure that alga cells wall is made up of multi-layer micro-fibers such as cellulose, pectic substance, Alginic acid ammonium salt polysaccharide and half poly lactose sulfuric esters, has bigger surface area. Simultaneously, the Gene therapies such as polysaccharide on cell wall, protein, phospholipid provide the functional group can being combined in a large number with metal ion to algae, such as amino, sulfenyl, sulfydryl, hydroxyl, carboxyl, imidazole radicals, sulfate radical, sulfate radical, phenol, aldehyde radical, amide groups etc., these functional groups' energy Rational Arrangements are on the alga cells wall with larger surface. Being fully contacted with metal ion, some of which can lose proton and electronegative, by electrostatic attraction adsorbing metal ions; Some band lone pair electrons, can form coordinate bond and Absorptive complex wave metal ion with metal ion. Meanwhile, also with certain electric charge and viscosity on cell wall, further increase it to metal biosorption ability.
Porphyridium cruentum is as the original Rhodophyta unicellular alga of a kind of comparison, it is possible to produce many bioactive substances, such as phycobniliprotein, polyunsaturated fatty acid and extracellular polysaccharide material. The polymer that the monosaccharide such as Porphyridium cruentum cell can accumulate the polysaccharide of 20%-50% Biomass, and this polysaccharide is by xylose, glucose, galactose are constituted, has the colloidal property of uniqueness, and viscosity is big, its structure and Algin, and laminaran is similar.
Based on the absorbability that alga cells is good, and there is adsorption rate soon, do not cause the plurality of advantages of secondary pollution.So in recent years the research of algae extracellular polysaccharide being increased gradually. But yield poorly, adsorption capacity is little, resistance is poor and regeneration capacity is poor, it is still the leading factor restricting its large-scale production.
Because above-mentioned defect, the design people, actively in addition research and innovation, to the preparation method founding Porphyridium cruentum extracellular polysaccharide adsorbent.
Summary of the invention
For solving above-mentioned technical problem, it is an object of the invention to provide Porphyridium cruentum extracellular polysaccharide adsorbent and preparation method thereof. This preparation method includes the separating-purifying process of Porphyridium cruentum extracellular polysaccharide, the sulfonation process of Porphyridium cruentum extracellular polysaccharide and the immobilization of Porphyridium cruentum extracellular polysaccharide and processes. By the heavy metal ion in the adsorbent optionally adsorbed water body that the method prepares and precious metal ion, and Porphyridium cruentum extracellular polysaccharide sorbent reactions is rapid, and adsorption capacity is big, and clearance is high, and is easy to eluting. Can Reusability, decrease the secondary pollution to environment, environmental friendliness; Reduce and pollute the ecological risk that residual causes with biological species introduction.
This invention address that the technical scheme of described technical problem is to provide Porphyridium cruentum extracellular polysaccharide adsorbent, it is characterized in that described adsorbent is prepared in the following manner:
(1) separating-purifying of Porphyridium cruentum extracellular polysaccharide processes:
1) taking fresh Porphyridium cruentum and add distilled water or phosphate buffer solution mixing, carry out ultrasonication, the pH value of described phosphate buffer solution is 7.5, and concentration is 40～105mmol/L;
2) by step 1) the broken liquid that obtains is centrifuged, abandons precipitation, takes supernatant;
3) by step 2) supernatant that obtains sloughs albumen;
4) by step 3) ethanol that adds 2～3 times of volumes in the supernatant sloughing albumen that obtains precipitates, centrifugal collecting precipitation, washing with acetone, dry, obtains crude polysaccharides;
5) step 4 is taken) crude polysaccharides that obtains is dissolved in distilled water or tris-HCI buffer, make saturated solution, it is centrifuged and abandons precipitation, supernatant is splined on DEAE-SepharoseFastFlow post, with Tris-HCl for eluent, carry out NaCl gradient elution, phend-sulphuric acid tracing detection, collect simple spike, it is splined on sephadexG-100 or sephadexG-75 post after dialysis deionization, carrying out eluting with deionized water, collect simple spike, lyophilization obtains purification Porphyridium cruentum extracellular polysaccharide;
(2) sulfonation of Porphyridium cruentum extracellular polysaccharide processes: by step 5) the purification Porphyridium cruentum extracellular polysaccharide that obtains is in inert gas environment, with DMF or DMAc for solvent, response time 1-3h, temperature 80-110 DEG C, carry out sulfonation process by sulfonated reagent, obtain the Porphyridium cruentum extracellular polysaccharide after sulfonation processes;
(3) immobilization of Porphyridium cruentum extracellular polysaccharide processes: the carrier of the Porphyridium cruentum extracellular polysaccharide after processing using polyacrylamide, sodium alginate or silica gel as sulfonation, realize the immobilization of Porphyridium cruentum extracellular polysaccharide is processed, finally give the Porphyridium cruentum extracellular polysaccharide adsorbent with high adsorption and high stability.
Described sulfonated reagent is selected from SO3One in-pyridine complex, chlorosulfonic acid, piperidines-N-sulfonic acid, concentrated sulphuric acid, oleum, chlorosulfonic acid-pyridine complex and chlorosulfonic acid-boranepiperidine complex.
The preparation method that present invention also offers described Porphyridium cruentum extracellular polysaccharide adsorbent, is characterized in that described preparation method specifically comprises the following steps that
(1) separating-purifying of Porphyridium cruentum extracellular polysaccharide processes:
1) taking fresh Porphyridium cruentum and add distilled water or phosphate buffer solution mixing, carry out ultrasonication, the pH value of described phosphate buffer solution is 7.5, and concentration is 40～105mmol/L;
2) by step 1) the broken liquid that obtains is centrifuged, abandons precipitation, takes supernatant;
3) by step 2) supernatant that obtains sloughs albumen;
4) by step 3) ethanol that adds 2～3 times of volumes in the supernatant sloughing albumen that obtains precipitates, centrifugal collecting precipitation, washing with acetone, dry, obtains crude polysaccharides;
5) step 4 is taken) crude polysaccharides that obtains is dissolved in distilled water or tris-HCI buffer, make saturated solution, it is centrifuged and abandons precipitation, supernatant is splined on DEAE-SepharoseFastFlow post, with Tris-HCl for eluent, carry out NaCl gradient elution, phend-sulphuric acid tracing detection, collect simple spike, it is splined on sephadexG-100 or sephadexG-75 post after dialysis deionization, carrying out eluting with deionized water, collect simple spike, lyophilization obtains purification Porphyridium cruentum extracellular polysaccharide;
(2) sulfonation of Porphyridium cruentum extracellular polysaccharide processes: by step 5) the purification Porphyridium cruentum extracellular polysaccharide that obtains is in inert gas environment, with DMF or DMAc for solvent, response time 1-3h, temperature 80-110 DEG C, carry out sulfonation process by sulfonated reagent, obtain the Porphyridium cruentum extracellular polysaccharide after sulfonation processes;
(3) immobilization of Porphyridium cruentum extracellular polysaccharide processes: the carrier of the Porphyridium cruentum extracellular polysaccharide after processing using polyacrylamide, sodium alginate or silica gel as sulfonation, realize the immobilization of Porphyridium cruentum extracellular polysaccharide is processed, finally give the Porphyridium cruentum extracellular polysaccharide adsorbent with high adsorption and high stability;
Described sulfonated reagent is selected from SO3One in-pyridine complex, chlorosulfonic acid, piperidines-N-sulfonic acid, concentrated sulphuric acid, oleum, chlorosulfonic acid-pyridine complex and chlorosulfonic acid-boranepiperidine complex.
Compared with prior art, the beneficial effects of the present invention is:
(1) the Porphyridium cruentum algae kind that acquisition growth is fast, strong stress resistance, polysaccharide yield are high is adopted;
(2) heavy metal ion in the Porphyridium cruentum extracellular polysaccharide adsorbent optionally adsorbed water body produced and precious metal ion, and Porphyridium cruentum extracellular polysaccharide sorbent reactions is rapid, adsorption capacity is big, and clearance is high, and is easy to eluting. Porphyridium cruentum extracellular polysaccharide adsorbent can Reusability, decrease the secondary pollution to environment, environmental friendliness; Reduce and pollute the ecological risk that residual causes with biological species introduction;
(3) present invention adopts the separation purification sulfonation of Porphyridium cruentum extracellular polysaccharide, and finally obtains immobilized biological adsorbent technology, efficiently solves that the adsorption efficiency that current chemosorbent produces is low, eluting is difficult, easily cause the problem of secondary pollution;
Detailed description of the invention
Below in conjunction with embodiment, the specific embodiment of the present invention is described in further detail. Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.
Embodiment 1
(1) separating-purifying of Porphyridium cruentum extracellular polysaccharide processes:
1) taking fresh Porphyridium cruentum and add distilled water or phosphate buffer solution mixing, carry out ultrasonication, the pH value of described phosphate buffer solution is 7.5, and concentration is 60mmol/L;
2) by step 1) the broken liquid that obtains is centrifuged, abandons precipitation, takes supernatant;
3) by step 2) supernatant that obtains sloughs albumen;
4) by step 3) ethanol that adds 2 times of volumes in the supernatant sloughing albumen that obtains precipitates, centrifugal collecting precipitation, washing with acetone, dry, obtains crude polysaccharides;
5) step 4 is taken) crude polysaccharides that obtains is dissolved in distilled water, make saturated solution, be centrifuged and abandon precipitation, supernatant is splined on DEAE-SepharoseFastFlow post, with Tris-HCl for eluent, carry out NaCl gradient elution, phend-sulphuric acid tracing detection, collect simple spike, it is splined on sephadexG-75 post after dialysis deionization, carrying out eluting with deionized water, collect simple spike, lyophilization obtains purification Porphyridium cruentum extracellular polysaccharide;
(2) sulfonation of Porphyridium cruentum extracellular polysaccharide processes: by step 5) the purification Porphyridium cruentum extracellular polysaccharide that obtains is in inert gas environment, with DMF for solvent, response time 1h, temperature 80 DEG C, pass through SO3-pyridine complex carries out sulfonation process, obtains the Porphyridium cruentum extracellular polysaccharide after sulfonation processes;
(3) immobilization of Porphyridium cruentum extracellular polysaccharide processes: the carrier of the Porphyridium cruentum extracellular polysaccharide after processing using polyacrylamide as sulfonation, realize the immobilization of Porphyridium cruentum extracellular polysaccharide is processed, finally give the Porphyridium cruentum extracellular polysaccharide adsorbent with high adsorption and high stability.
Embodiment 2
(1) separating-purifying of Porphyridium cruentum extracellular polysaccharide processes:
1) taking fresh Porphyridium cruentum and add distilled water or phosphate buffer solution mixing, carry out ultrasonication, the pH value of described phosphate buffer solution is 7.5, and concentration is 100mmol/L;
2) by step 1) the broken liquid that obtains is centrifuged, abandons precipitation, takes supernatant;
3) by step 2) supernatant that obtains sloughs albumen;
4) by step 3) ethanol that adds 3 times of volumes in the supernatant sloughing albumen that obtains precipitates, centrifugal collecting precipitation, washing with acetone, dry, obtains crude polysaccharides;
5) step 4 is taken) crude polysaccharides that obtains is dissolved in tris-HCI buffer, make saturated solution, be centrifuged and abandon precipitation, supernatant is splined on DEAE-SepharoseFastFlow post, with Tris-HCl for eluent, carry out NaCl gradient elution, phend-sulphuric acid tracing detection, collect simple spike, it is splined on sephadexG-100 post after dialysis deionization, carrying out eluting with deionized water, collect simple spike, lyophilization obtains purification Porphyridium cruentum extracellular polysaccharide;
(2) sulfonation of Porphyridium cruentum extracellular polysaccharide processes: by step 5) the purification Porphyridium cruentum extracellular polysaccharide that obtains is in inert gas environment, with DMAc for solvent, response time 2h, temperature 90 DEG C, carry out sulfonation process by piperidines-N-sulfonic acid, obtain the Porphyridium cruentum extracellular polysaccharide after sulfonation processes;
(3) immobilization of Porphyridium cruentum extracellular polysaccharide processes: the carrier of the Porphyridium cruentum extracellular polysaccharide after processing using sodium alginate as sulfonation, realize the immobilization of Porphyridium cruentum extracellular polysaccharide is processed, finally give the Porphyridium cruentum extracellular polysaccharide adsorbent with high adsorption and high stability.
Embodiment 3
(1) separating-purifying of Porphyridium cruentum extracellular polysaccharide processes:
1) taking fresh Porphyridium cruentum and add distilled water or phosphate buffer solution mixing, carry out ultrasonication, the pH value of described phosphate buffer solution is 7.5, and concentration is 40mmol/L;
2) by step 1) the broken liquid that obtains is centrifuged, abandons precipitation, takes supernatant;
3) by step 2) supernatant that obtains sloughs albumen;
4) by step 3) ethanol that adds 2.5 times of volumes in the supernatant sloughing albumen that obtains precipitates, centrifugal collecting precipitation, washing with acetone, dry, obtains crude polysaccharides;
5) step 4 is taken) crude polysaccharides that obtains is dissolved in tris-HCI buffer, make saturated solution, be centrifuged and abandon precipitation, supernatant is splined on DEAE-SepharoseFastFlow post, with Tris-HCl for eluent, carry out NaCl gradient elution, phend-sulphuric acid tracing detection, collect simple spike, it is splined on sephadexG-100 post after dialysis deionization, carrying out eluting with deionized water, collect simple spike, lyophilization obtains purification Porphyridium cruentum extracellular polysaccharide;
(2) sulfonation of Porphyridium cruentum extracellular polysaccharide processes: by step 5) the purification Porphyridium cruentum extracellular polysaccharide that obtains is in inert gas environment, with DMF for solvent, response time 3h, temperature 110 DEG C, carry out sulfonation process by chlorosulfonic acid-boranepiperidine complex, obtain the Porphyridium cruentum extracellular polysaccharide after sulfonation processes;
(3) immobilization of Porphyridium cruentum extracellular polysaccharide processes: the carrier of the Porphyridium cruentum extracellular polysaccharide after processing using silica gel as sulfonation, realize the immobilization of Porphyridium cruentum extracellular polysaccharide is processed, finally give the Porphyridium cruentum extracellular polysaccharide adsorbent with high adsorption and high stability;
Embodiment 4
(1) separating-purifying of Porphyridium cruentum extracellular polysaccharide processes:
1) taking fresh Porphyridium cruentum and add distilled water or phosphate buffer solution mixing, carry out ultrasonication, the pH value of described phosphate buffer solution is 7.5, and concentration is 70mmol/L;
2) by step 1) the broken liquid that obtains is centrifuged, abandons precipitation, takes supernatant;
3) by step 2) supernatant that obtains sloughs albumen;
4) by step 3) ethanol that adds 2 times of volumes in the supernatant sloughing albumen that obtains precipitates, centrifugal collecting precipitation, washing with acetone, dry, obtains crude polysaccharides;
5) step 4 is taken) crude polysaccharides that obtains is dissolved in distilled water buffer, make saturated solution, be centrifuged and abandon precipitation, supernatant is splined on DEAE-SepharoseFastFlow post, with Tris-HCl for eluent, carry out NaCl gradient elution, phend-sulphuric acid tracing detection, collect simple spike, it is splined on sephadexG-100 post after dialysis deionization, carrying out eluting with deionized water, collect simple spike, lyophilization obtains purification Porphyridium cruentum extracellular polysaccharide;
(2) sulfonation of Porphyridium cruentum extracellular polysaccharide processes: by step 5) the purification Porphyridium cruentum extracellular polysaccharide that obtains is in inert gas environment, with DMF for solvent, response time 2h, temperature 85 DEG C, carry out sulfonation process by chlorosulfonic acid-pyridine complex, obtain the Porphyridium cruentum extracellular polysaccharide after sulfonation processes;
(3) immobilization of Porphyridium cruentum extracellular polysaccharide processes: the carrier of the Porphyridium cruentum extracellular polysaccharide after processing using polyacrylamide as sulfonation, realize the immobilization of Porphyridium cruentum extracellular polysaccharide is processed, finally give the Porphyridium cruentum extracellular polysaccharide adsorbent with high adsorption and high stability;
By adopting the electroplating wastewater of Tianjin lighting Co. Ltd. to test, the technical specification that the present invention reaches is:
A. the recovering effect of low content precious metal in Adsorbent For Removal of Heavy: gold, silver, copper, nickel the response rate be all higher than 90%.
B. adsorbent reproducible utilization.
The above is only the preferred embodiment of the present invention; it is not limited to the present invention; should be understood that; for those skilled in the art; under the premise without departing from the technology of the present invention principle; can also making some improvement and modification, these improve and modification also should be regarded as protection scope of the present invention.
Claims (3)
1. Porphyridium cruentum extracellular polysaccharide adsorbent, is characterized in that described adsorbent is prepared in the following manner:
(1) separating-purifying of Porphyridium cruentum extracellular polysaccharide processes:
1) taking fresh Porphyridium cruentum and add distilled water or phosphate buffer solution mixing, carry out ultrasonication, the pH value of described phosphate buffer solution is 7.5, and concentration is 40～105mmol/L;
2) by step 1) the broken liquid that obtains is centrifuged, abandons precipitation, takes supernatant;
3) by step 2) supernatant that obtains sloughs albumen;
4) by step 3) ethanol that adds 2～3 times of volumes in the supernatant sloughing albumen that obtains precipitates, centrifugal collecting precipitation, washing with acetone, dry, obtains crude polysaccharides;
5) step 4 is taken) crude polysaccharides that obtains is dissolved in distilled water or tris-HCI buffer, make saturated solution, it is centrifuged and abandons precipitation, supernatant is splined on DEAE-SepharoseFastFlow post, with Tris-HCl for eluent, carry out NaCl gradient elution, phend-sulphuric acid tracing detection, collect simple spike, it is splined on sephadexG-100 or sephadexG-75 post after dialysis deionization, carrying out eluting with deionized water, collect simple spike, lyophilization obtains purification Porphyridium cruentum extracellular polysaccharide;
(2) sulfonation of Porphyridium cruentum extracellular polysaccharide processes: by step 5) the purification Porphyridium cruentum extracellular polysaccharide that obtains is in inert gas environment, with DMF or DMAc for solvent, response time 1-3h, temperature 80-110 DEG C, carry out sulfonation process by sulfonated reagent, obtain the Porphyridium cruentum extracellular polysaccharide after sulfonation processes;
(3) immobilization of Porphyridium cruentum extracellular polysaccharide processes: the carrier of the Porphyridium cruentum extracellular polysaccharide after processing using polyacrylamide, sodium alginate or silica gel as sulfonation, it is achieved the immobilization process of Porphyridium cruentum extracellular polysaccharide is obtained Porphyridium cruentum extracellular polysaccharide adsorbent.
2. Porphyridium cruentum extracellular polysaccharide adsorbent according to claim 1, it is characterised in that the sulfonated reagent that the immobilization of described Porphyridium cruentum extracellular polysaccharide processes one in SO3-pyridine complex, chlorosulfonic acid, piperidines-N-sulfonic acid, concentrated sulphuric acid, oleum, chlorosulfonic acid-pyridine complex and chlorosulfonic acid-boranepiperidine complex.
3. the preparation method of the Porphyridium cruentum extracellular polysaccharide adsorbent as described in as arbitrary in claim 1 or 2, is characterized in that described preparation method specifically comprises the following steps that
(1) separating-purifying of Porphyridium cruentum extracellular polysaccharide processes:
1) taking fresh Porphyridium cruentum and add distilled water or phosphate buffer solution mixing, carry out ultrasonication, the pH value of described phosphate buffer solution is 7.5, and concentration is 40～105mmol/L;
2) by step 1) the broken liquid that obtains is centrifuged, abandons precipitation, takes supernatant;
3) by step 2) supernatant that obtains sloughs albumen;
4) by step 3) ethanol that adds 2～3 times of volumes in the supernatant sloughing albumen that obtains precipitates, centrifugal collecting precipitation, washing with acetone, dry, obtains crude polysaccharides;
5) step 4 is taken) crude polysaccharides that obtains is dissolved in distilled water or tris-HCI buffer, make saturated solution, it is centrifuged and abandons precipitation, supernatant is splined on DEAE-SepharoseFastFlow post, with Tris-HCl for eluent, carry out NaCl gradient elution, phend-sulphuric acid tracing detection, collect simple spike, it is splined on sephadexG-100 or sephadexG-75 post after dialysis deionization, carrying out eluting with deionized water, collect simple spike, lyophilization obtains purification Porphyridium cruentum extracellular polysaccharide;
(2) sulfonation of Porphyridium cruentum extracellular polysaccharide processes: by step 5) the purification Porphyridium cruentum extracellular polysaccharide that obtains is in inert gas environment, with DMF or DMAc for solvent, response time 1-3h, temperature 80-110 DEG C, carry out sulfonation process by sulfonated reagent, obtain the Porphyridium cruentum extracellular polysaccharide after sulfonation processes;
(3) immobilization of Porphyridium cruentum extracellular polysaccharide processes: the carrier of the Porphyridium cruentum extracellular polysaccharide after processing using polyacrylamide, sodium alginate or silica gel as sulfonation, it is achieved the immobilization process of Porphyridium cruentum extracellular polysaccharide is obtained Porphyridium cruentum extracellular polysaccharide adsorbent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410664269.XA CN105664862A (en) | 2014-11-20 | 2014-11-20 | Porphyridium extracellular polysaccharide adsorbent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410664269.XA CN105664862A (en) | 2014-11-20 | 2014-11-20 | Porphyridium extracellular polysaccharide adsorbent and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105664862A true CN105664862A (en) | 2016-06-15 |
Family
ID=56944951
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410664269.XA Pending CN105664862A (en) | 2014-11-20 | 2014-11-20 | Porphyridium extracellular polysaccharide adsorbent and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105664862A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107055720A (en) * | 2017-03-31 | 2017-08-18 | 嘉兴学院 | A kind of sulfonation modifying biological flocculant and its preparation method and application |
| CN107285478A (en) * | 2017-06-21 | 2017-10-24 | 浙江海洋大学 | The absorption that a kind of drinking water heavy metal depth is removed brews agent |
| CN107383222A (en) * | 2017-06-21 | 2017-11-24 | 磐安县派普特生物科技有限公司 | For the preparation method for the ball algae extract for improving immunity |
| CN107626285A (en) * | 2017-09-18 | 2018-01-26 | 浙江海洋大学 | A kind of method for preparing heavy metal absorbent using the cold Bacillus genus strain in ocean |
| CN108246273A (en) * | 2018-02-08 | 2018-07-06 | 天津大学 | Sulfonated sodium alginate grafting Ago-Gel chromatographic media and preparation method and application |
| CN115554990A (en) * | 2022-09-22 | 2023-01-03 | 四川大学 | Nano starch soft particle modified macroporous polysaccharide medium for improving protein adsorption performance |
-
2014
- 2014-11-20 CN CN201410664269.XA patent/CN105664862A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107055720A (en) * | 2017-03-31 | 2017-08-18 | 嘉兴学院 | A kind of sulfonation modifying biological flocculant and its preparation method and application |
| CN107285478A (en) * | 2017-06-21 | 2017-10-24 | 浙江海洋大学 | The absorption that a kind of drinking water heavy metal depth is removed brews agent |
| CN107383222A (en) * | 2017-06-21 | 2017-11-24 | 磐安县派普特生物科技有限公司 | For the preparation method for the ball algae extract for improving immunity |
| CN107285478B (en) * | 2017-06-21 | 2020-08-25 | 浙江海洋大学 | Adsorption foam flushing agent for deeply removing heavy metals in drinking water |
| CN107626285A (en) * | 2017-09-18 | 2018-01-26 | 浙江海洋大学 | A kind of method for preparing heavy metal absorbent using the cold Bacillus genus strain in ocean |
| CN108246273A (en) * | 2018-02-08 | 2018-07-06 | 天津大学 | Sulfonated sodium alginate grafting Ago-Gel chromatographic media and preparation method and application |
| CN108246273B (en) * | 2018-02-08 | 2021-01-22 | 天津大学 | Sulfonated sodium alginate grafted agarose gel chromatographic medium, preparation method and application |
| CN115554990A (en) * | 2022-09-22 | 2023-01-03 | 四川大学 | Nano starch soft particle modified macroporous polysaccharide medium for improving protein adsorption performance |
| CN115554990B (en) * | 2022-09-22 | 2023-10-27 | 四川大学 | Preparation method of nano-starch modified macroporous polysaccharide chromatographic medium |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105664862A (en) | Porphyridium extracellular polysaccharide adsorbent and preparation method thereof | |
| CN101928828B (en) | Method for extracting lithium from salt lake brine by adsorption method | |
| CN104289188B (en) | A kind of preparation of the modified Pericarpium Musae adsorbent of pyromellitic acid anhydride | |
| CN105344331A (en) | Spherical heavy metal capture and adsorption material and preparation method thereof | |
| CN103693711A (en) | Method for treating nickel/copper-containing electroplating wastewater by using weak acid ion exchange fibers | |
| CN103614916B (en) | A kind of SPE composite nano-fiber material preparation method | |
| CN103566908B (en) | A kind of preparation method of pyromellitic acid anhydride modification coconut shell adsorbent and application | |
| CN106632519B (en) | Process for separating nucleotide by adopting continuous ion exchange chromatography technology | |
| CN111905695A (en) | Cellulose-based hollow porous carbon fiber microsphere adsorbent and preparation method thereof | |
| CN102908998B (en) | Preparation method of xanthate macro-pore dextrangel adsorbent | |
| Haijia et al. | Biosorption of Ni2+ by the surface molecular imprinting adsorbent | |
| CN104353424A (en) | Amine-containing functional mesoporous material and application thereof to separation of rhenium and molybdenum | |
| CN103191702A (en) | Preparation method and application of sulfydryl palm bark adsorbent | |
| CN104815565A (en) | Preparation method for compound cellulose film for adsorbing heavy metal ions | |
| CN110642962B (en) | Separation and purification method of hybrid bean pectin polysaccharide | |
| CN105363417A (en) | Preparation method for cross-linked carboxymethylated agarose-base gel microsphere | |
| CN106861648A (en) | A kind of preparation method and product for vulcanizing chelate modified stalk fibre adsorbent | |
| CN108752405A (en) | A kind of method of ion exchange resin combination chromatography separating nucleotide | |
| CN103566904A (en) | Cellulose-chitosan compound and preparation method thereof | |
| CN104045725B (en) | D301G resin anion(R.A) is adopted to refine the method for Phaeopoms obliquus Crude polysaccharides | |
| CN101085797B (en) | Cryogel adsorption chromatography separation method for adenosine triphosphate | |
| CN102228823B (en) | Modified beer waste yeast adsorbent, preparation method and application thereof | |
| CN108707248B (en) | Preparation method of flame-retardant super-hydrophobic melamine sponge | |
| CN109852591A (en) | A kind of method that soya whey wastewater extracts lipoxygenase against pH gradient | |
| CN103331146B (en) | Modified bean pulp adsorbent as well as preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160615 |