CN105664243B - A kind of three-dimensional rack and preparation method thereof of polylactic acid nano micron fibre structure - Google Patents
A kind of three-dimensional rack and preparation method thereof of polylactic acid nano micron fibre structure Download PDFInfo
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- CN105664243B CN105664243B CN201610106725.8A CN201610106725A CN105664243B CN 105664243 B CN105664243 B CN 105664243B CN 201610106725 A CN201610106725 A CN 201610106725A CN 105664243 B CN105664243 B CN 105664243B
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- polylactic acid
- micron
- fibre structure
- dimensional rack
- acid nano
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- 239000000835 fiber Substances 0.000 title claims abstract description 49
- 239000004626 polylactic acid Substances 0.000 title claims abstract description 49
- 229920000747 poly(lactic acid) Polymers 0.000 title claims abstract description 48
- 238000002360 preparation method Methods 0.000 title abstract description 8
- 238000010041 electrostatic spinning Methods 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 16
- 150000002012 dioxanes Chemical class 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 9
- 238000009987 spinning Methods 0.000 claims description 9
- 241000209094 Oryza Species 0.000 claims description 5
- 235000007164 Oryza sativa Nutrition 0.000 claims description 5
- 235000009566 rice Nutrition 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 230000005611 electricity Effects 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 10
- 239000002253 acid Substances 0.000 abstract description 7
- 229920002101 Chitin Polymers 0.000 abstract 2
- 238000004108 freeze drying Methods 0.000 abstract 1
- 239000005030 aluminium foil Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 229920006381 polylactic acid film Polymers 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000002062 molecular scaffold Substances 0.000 description 1
- 239000002121 nanofiber Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
- D01D5/003—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4326—Condensation or reaction polymers
- D04H1/435—Polyesters
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Textile Engineering (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Medicinal Chemistry (AREA)
- Dermatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Mechanical Engineering (AREA)
- Materials For Medical Uses (AREA)
- Artificial Filaments (AREA)
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Abstract
The present invention relates to a kind of three-dimensional racks and preparation method thereof of polylactic acid nano micron fibre structure, it is used distinguishes electrostatic spinning nano and micron chitin fiber film under certain condition, by nanometer and micron chitin fiber film, high speed shear divides the three-dimensional rack of available polylactic acid nano micron fibre structure after sample, freeze forming and freeze-drying at staple fiber in water according to a certain ratio.The preparation method simple process, the acid fiber by polylactic of different-diameter can be obtained using method of electrostatic spinning, the ratio and concentration of adjustable nanometer and micrometer fibers during freeze forming, the polylactic acid being prepared receive micron three-dimensional rack with good biocompatibility and mechanical property.
Description
Technical field
The present invention relates to a kind of three-dimensional racks and preparation method thereof of polylactic acid nano micron fibre structure, belong to bio-medical
Field of material technology.
Background technique
A kind of synthetic polymer timbering material that polylactic acid is ratified earliest as U.S. FDA, have it is nontoxic, with human body phase
Capacitive is good, in vivo can degradable absorption the advantages that, be widely used in the tissues such as bone, cartilage, blood vessel and skin and device
Official reparation (Lee EJ etc., Ann Biomed Eng, 2014;42 (2): 323).The microstructure of bracket has its comprehensive performance
Large effect can enhance the absorption of protein research shows that nano fiber scaffold has biggish specific surface area and adsorption site,
The adsorption site for increasing cell adherence, to can promote the reparation of tissue and organ.But simple nanofiber mechanical strength compared with
It is low, it can not apply in the reparation of the more demanding tissue of mechanical strength and organ.In comparison, micrometer fibers bracket have compared with
Good mechanical property, and degradation rate is slow, the structural stability being able to maintain that in stent implantation procedure.Therefore, in the bracket
It there is nanometer and the microstructure of micrometer fibers then can overcome the contradiction between biocompatibility and mechanical property simultaneously.At present
The method that polylactic acid mostly uses Thermal inactive during preparing three-dimensional rack, using tetrahydrofuran or dioxanes equal solvent
Nanometer and micrometer fibers shape bracket can be respectively obtained, but the control of its fibre diameter is more difficult, while obtaining nanometer and micron
Fiber is then more difficult (CN103159977A).It is thin that the method that researcher passes through electrostatic spinning at present also can get acid fiber by polylactic
Film, but it is also very difficult for obtaining three-dimensional rack by method of electrostatic spinning.
Summary of the invention
Main imagination of the invention is to prepare nanometer and micron polylactic acid film respectively using the method for electrostatic spinning, by one
Fixed proportion mixes nanometer and micron polylactic acid fiber membrane, and by polylactic acid film high speed shear at staple fiber, freeze-dried
Obtain three-dimensional nano micron fibre polylactic acid bracket.
The preparation method of three wiener micron polylactic acid brackets is described in detail below in the present invention.
(1) electrostatic spinning nano polylactic acid fiber membrane, prepares the solution of polylactic acid 10.0%, and solvent is chloroform and dioxanes
Mixture, volume ratio 70:30, spinning voltage is between 20~25kV.
(2) electrostatic spinning micron polylactic acid fiber membrane, prepares the solution of polylactic acid 20.0%, and solvent is chloroform and dioxanes
Mixture, volume ratio 90:10, spinning voltage is between 12~15kV.
(3) quantitative nanometer and micron polylactic acid fiber membrane are weighed respectively, is added in quantitative water, in water high speed
Cut into staple fiber.
(4) divide sample into weighing bottle, the freeze forming at -18 DEG C, available polylactic acid is received micro- after being lyophilized in freeze dryer
The three-dimensional rack of rice fibre structure.
Polylactic acid molecule amount used above is between 5~100,000, the mass ratio of nanometer polylactic acid and micron polylactic acid
Between 20:80 to 80:20, the total concentration of polylactic acid nano micrometer fibers in water is between 1~3%.
The three-dimensional rack of polylactic acid nano micron fibre structure produced by the present invention is white disk, and shape and height can be
Divide in sample and uses different containers and sample volume is divided to adjust.The structure that nano micron fibre coexists assigns the good bio-compatible of bracket
Property and mechanical strength.The preparation method simple process is also convenient to adjustment spinning condition in preparation and obtains receiving for different-diameter
Rice and micron acid fiber by polylactic, compared to simple method of electrostatic spinning, which can easily prepare the bracket of three-dimensional structure.
Specific embodiment
Combined with specific embodiments below, the content of present invention is further described, but implementation of the invention is not
It is confined to this.
Embodiment 1: taking the chloroform of 7ml and the dioxanes of 3ml respectively, is uniformly mixed, and weighs 1.0g polylactic acid, stirs molten
Solution stands 12 hours.PLA solution is added in syringe, the voltage spinning of 20kV is passed to, collects the aluminium foil in ground connection
On, it can get acid fiber by polylactic diameter in the film of 50-750nm.The chloroform of 9ml and the dioxanes of 1ml are taken respectively, and mixing is equal
It is even, 2.0g polylactic acid is weighed, stirring and dissolving stands 12 hours.PLA solution is added in syringe, the electricity of 15kV is passed to
Pressure spinning silk is collected on the aluminium foil of ground connection, can get film of the acid fiber by polylactic diameter at 1-10 μm.Weigh respectively 0.2g and
0.8g nanometers and micron polylactic acid fiber membrane, cut into staple fiber in 100ml water high speed, divide sample into weighing bottle, at -18 DEG C
Lower freeze forming can get the three-dimensional rack of polylactic acid nano micron fibre structure after being lyophilized in freeze dryer.
Embodiment 2: taking the chloroform of 21ml and the dioxanes of 9ml respectively, is uniformly mixed, and weighs 3.0g polylactic acid, stirs molten
Solution stands 12 hours.PLA solution is added in syringe, the voltage spinning of 25kV is passed to, collects the aluminium foil in ground connection
On, it can get acid fiber by polylactic diameter in the film of 50-300nm.The chloroform of 9ml and the dioxanes of 1ml are taken respectively, and mixing is equal
It is even, 2.0g polylactic acid is weighed, stirring and dissolving stands 12 hours.PLA solution is added in syringe, the electricity of 12kV is passed to
Pressure spinning silk is collected on the aluminium foil of ground connection, can get film of the acid fiber by polylactic diameter at 1-10 μm.Weigh respectively 2.4g and
0.6g nanometers and micron polylactic acid fiber membrane, cut into staple fiber in 100ml water high speed, divide sample into weighing bottle, at -18 DEG C
Lower freeze forming can get the three-dimensional rack of polylactic acid nano micron fibre structure after being lyophilized in freeze dryer.
Claims (5)
1. a kind of three-dimensional rack of polylactic acid nano micron fibre structure, it is characterised in that electrostatic spinning is received respectively under certain condition
Rice and micron polylactic acid fiber membrane, according to a certain ratio by nanometer and micron polylactic acid fiber membrane in water high speed shear at short fibre
Dimension, divides sample and the freeze forming at -18 DEG C, can get the three-dimensional branch of polylactic acid nano micron fibre structure after being lyophilized in freeze dryer
Frame.
2. the three-dimensional rack of polylactic acid nano micron fibre structure according to claim 1, it is characterised in that used poly-
Lactic acid molecules amount is between 5~100,000.
3. the three-dimensional rack of polylactic acid nano micron fibre structure according to claim 1, it is characterised in that electrostatic spinning is received
When rice tunica fibrosa, PLA concentration 10.0%, solvent is the mixture of chloroform and dioxanes, volume ratio 70:30, spinning electricity
It is pressed between 20~25kV.
4. the three-dimensional rack of polylactic acid nano micron fibre structure according to claim 1, it is characterised in that electrostatic spinning is micro-
When rice polylactic acid fiber membrane, PLA concentration 20.0%, mixture of the solvent for chloroform and dioxanes, volume ratio 90:10,
Spinning voltage is between 12~15kV.
5. the three-dimensional rack of polylactic acid nano micron fibre structure according to claim 1, it is characterised in that nanometer polylactic acid
And the mass ratio of micron polylactic acid is between 20:80 to 80:20, the total concentration of polylactic acid nano micrometer fibers in water 1~
Between 3%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610106725.8A CN105664243B (en) | 2016-02-27 | 2016-02-27 | A kind of three-dimensional rack and preparation method thereof of polylactic acid nano micron fibre structure |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610106725.8A CN105664243B (en) | 2016-02-27 | 2016-02-27 | A kind of three-dimensional rack and preparation method thereof of polylactic acid nano micron fibre structure |
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| Publication Number | Publication Date |
|---|---|
| CN105664243A CN105664243A (en) | 2016-06-15 |
| CN105664243B true CN105664243B (en) | 2018-12-04 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103285424A (en) * | 2013-05-27 | 2013-09-11 | 东华大学 | Three-dimensional fiber-based aerogel tissue engineering scaffold and preparation method thereof |
| CN104888278A (en) * | 2015-05-20 | 2015-09-09 | 东华大学 | Nanometer/micrometer fiber three-dimensional porous structure support material and preparation and application of support material |
| CN105107022A (en) * | 2015-09-21 | 2015-12-02 | 东华大学 | Preparation method for nanofiber porous scaffold having compression elasticity in wet state |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2967833A4 (en) * | 2013-03-14 | 2016-11-09 | Lifenet Health | Aligned fiber and method of use thereof |
| CN103691005B (en) * | 2013-12-24 | 2015-10-28 | 华东交通大学 | A kind of micro--Na fibrous tissue engineering rack and preparation method thereof |
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2016
- 2016-02-27 CN CN201610106725.8A patent/CN105664243B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103285424A (en) * | 2013-05-27 | 2013-09-11 | 东华大学 | Three-dimensional fiber-based aerogel tissue engineering scaffold and preparation method thereof |
| CN104888278A (en) * | 2015-05-20 | 2015-09-09 | 东华大学 | Nanometer/micrometer fiber three-dimensional porous structure support material and preparation and application of support material |
| CN105107022A (en) * | 2015-09-21 | 2015-12-02 | 东华大学 | Preparation method for nanofiber porous scaffold having compression elasticity in wet state |
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