CN105661114A - Plasma degradation method of aflatoxin B1 - Google Patents
Plasma degradation method of aflatoxin B1 Download PDFInfo
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- CN105661114A CN105661114A CN201410651104.9A CN201410651104A CN105661114A CN 105661114 A CN105661114 A CN 105661114A CN 201410651104 A CN201410651104 A CN 201410651104A CN 105661114 A CN105661114 A CN 105661114A
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Abstract
The present invention provides a plasma degradation method of aflatoxin B1. The steps of the plasma degradation method are as follows: aflatoxin is prepared into solutions with appropriate treating concentrations; the prepared aflatoxin solution is subjected to plasma treatment under a power of 100-300 W for 2-10 min; and after the treatment, degraded aflatoxins are obtained and a degradation rate is measured. A plasma generator uses vacuum plasma to conduct active particle bombardment on aflatoxin B1 in acetonitrile and conduct degradation by destroying aflatoxin active sites. The plasma degradation method has the three significant advantages: first, the method only uses plasma to treat aflatoxin B1, no other chemical agents are needed in the aflatoxin degradation process, so that no safety risk exists and the method is high in safety; secondly, the operation is simple and convenient; and thirdly, the method is free of pollution and an environment-friendly modification method.
Description
Technical field
The present invention relates to the new biodegrading process of toxin, particularly relate to a kind of plasma degradation method of AFB1.
Background technology
Aflatoxin be a class by the mycetogenetic secondary metabolite such as Aspergillus flavus and aspergillus parasiticus, be have been found that the one that mycotoxin toxic is maximum, there is extremely strong toxicity and carcinogenecity. The basic structure of AFB1 is two furan nucleuss and coumarin, and molecular weight 312 is soluble in acetonitrile, methanol, ethanol, chloroform, oil, is insoluble in water, insoluble in ether, petroleum ether, hexane.
Plasma technique is widely used to various fields as a kind of emerging technology. Nickelson etc. utilize high energy electron to process the waste liquid containing benzene and aromatic hydrocarbon, reach the organic purpose of fast degradation by producing substantial amounts of free radical and strong oxidizing property molecule; The research such as Patel uses hydrogen peroxide and gamma ray to irradiate the toxicity that then can more effectively reduce AFT simultaneously, as the gamma ray of 4kGy and the hydrogen peroxide of 5% can make the AFT complete inactivation of 100ug.
With acetonitrile for medium, measure AFB1 content, research different disposal time, plasma power, the different initial concentration impact on low temperature radio frequency plasma degrading aflatoxin B 1 effect by HPLC method.
Summary of the invention
The order of the present invention is the deficiency for existing toxin biodegrading process, offer physically based deformation processes, no solvent residue, AFB1 efficient, simple and direct detoxicating method, the AFB1 that the method can effectively be degraded in acetonitrile, and without any chemical composition, safety is high, and this method is simple to operate simultaneously, and environment does not cause any pollution.
In order to solve above-mentioned technical problem, the present invention is achieved by the following technical solutions:
Compared with prior art, advantages of the present invention and have the technical effect that the present invention is by simple reaction system, utilize vacuum plasma that AFB1 is bombarded, by the avtive spot of the action breaks down aflatoxin such as the active particle produced and free radical. The present invention has three remarkable advantages: one is that AFB1 is carried out radiation treatment by this method, need not add other chemical agent, be absent from chemical agent residue in processing procedure, and safety is high;Two is simple to operation; Three is pollution-free, is a kind of environmentally friendly method of modifying.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention will be further described, but these examples do not limit the scope of the invention. In addition any change that the present invention is done by those of ordinary skill in the related art, without departing from the essence of the present invention, all drops on equivalence in claims of the present invention limited range.
Embodiment 1
AFB1 used in the present invention is Israel's import high-purity toxin (> 99.99%).
Plasma apparatus used in the present invention is the radio-frequency (RF) plasma generator that model is Cesar1310 that AE PLC of the U.S. manufactures.
The compound vacuum gauge that the present invention can use the model that Chengdu Zheng Hua Electronic Instrument, Limited produces to be ZDF-III-LED controls pressure.
In the specific embodiment of the invention, degradation rate is Ct/C0(Ct is the concentration of t toxin, and C0 is the concentration of initial time toxin).
Blank group: AFB1 is without RF plasma processing, and all the other steps are all identical with experimental group.
Experimental group: AFB1 is made into 2ppm, three kinds of variable concentrations of 10ppm and 50ppm, the toxin soiutions prepared is placed on the pallet of plasma generator in weighing botle, the power supply of connection radio frequency plasma generating equipment and compound vacuum gauge, open vacuum pump and slowly open the diaphragm valve evacuation connected on vacuum pump, the process time simultaneously arranging plasma is 10min, processing power is respectively 200W, plasma generation button is pressed when vacuum reaches 15Pa, process completes post plasma generating means and is automatically stopped work, now slowly open reset valve, when vacuometer to be composite display occurs cavity pressure to reach 105Pa, weighing botle is taken out, process completes. the degradation rate best result obtaining aflatoxin is not 79.1%, 80.4% and 78.7%.
Embodiment 2
AFB1 is made into the solution of 10ppm concentration, the toxin soiutions prepared is placed on the pallet of plasma generator in weighing botle, the power supply of connection radio frequency plasma generating equipment and compound vacuum gauge, open vacuum pump and slowly open the diaphragm valve evacuation connected on vacuum pump, the process time simultaneously arranging plasma is 10min, processing power is respectively 100W, 200W and 300W, plasma generation button is pressed when vacuum reaches 15Pa, process completes post plasma generating means and is automatically stopped work, now slowly open reset valve, when vacuometer to be composite display occurs cavity pressure to reach 105Pa, weighing botle is taken out, process completes, the degradation rate best result obtaining aflatoxin is not 58.2%, 80.4% and 88.3%.
Embodiment 3
AFB1 is made into the solution of 10ppm concentration, the toxin soiutions prepared is placed on the pallet of plasma generator in weighing botle, the power supply of connection radio frequency plasma generating equipment and compound vacuum gauge, open vacuum pump and slowly open the diaphragm valve evacuation connected on vacuum pump, the process time simultaneously arranging plasma is 10min, processing power is respectively 200W, plasma generation button is pressed when vacuum reaches 15Pa, process completes post plasma generating means and is automatically stopped work, now slowly open reset valve, when vacuometer to be composite display occurs cavity pressure to reach 105Pa, weighing botle is taken out, process completes.Arranging the device of logical oxygen, all the other steps are identical with vacuum process simultaneously. The degradation rate best result obtaining aflatoxin is not 80.4% and 87.2%.
Above example is only in order to illustrate technical scheme, but not is limited; Although the present invention being described in detail with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or wherein portion of techniques feature is carried out equivalent replacement; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of present invention technical scheme required for protection.
Claims (5)
1. the plasma degradation method of AFB1 one kind novel, it is characterized in that, (1) toxin is made into a series of suitable concentration, (2) the variable concentrations toxin time of carrying out that step (1) processes gained is 2min ~ 10min, power is the Cement Composite Treated by Plasma of 100W ~ 300W, and (3) are disposed the AFB1 after being degraded.
2. plasma degradation method according to claim 1, it is characterised in that AFB1 solution is dissolved by acetonitrile and is made in step (1).
3. the method for Cement Composite Treated by Plasma AFB1 according to claim 1, it is characterised in that processing in step (2) plasma is process by radio-frequency (RF) plasma generator.
4. plasma degradation method according to claim 1, it is characterised in that processing mode is vacuum plasma treatment in step (2), and processing pressure controls at below 15Pa.
5. the method for Cement Composite Treated by Plasma AFB1 according to claim 1, it is characterised in that described Stress control be the following is at 15Pa and realized by compound vacuum gauge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410651104.9A CN105661114A (en) | 2014-11-17 | 2014-11-17 | Plasma degradation method of aflatoxin B1 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410651104.9A CN105661114A (en) | 2014-11-17 | 2014-11-17 | Plasma degradation method of aflatoxin B1 |
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| CN105661114A true CN105661114A (en) | 2016-06-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410651104.9A Pending CN105661114A (en) | 2014-11-17 | 2014-11-17 | Plasma degradation method of aflatoxin B1 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110698534A (en) * | 2019-10-25 | 2020-01-17 | 郑州轻工业学院 | Method for improving functional characteristics of peanut meal protein and degrading aflatoxin |
| CN110907241A (en) * | 2019-11-19 | 2020-03-24 | 上海市农业科学院 | Method for degrading deoxynivalenol in aqueous solution by using plasma |
| EP4406420A1 (en) | 2023-01-26 | 2024-07-31 | Jozef Stefan Institute | A method and a device for the degradation of aflatoxins using vacuum ultraviolet radiation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485408A (en) * | 2009-03-02 | 2009-07-22 | 中国农业科学院农产品加工研究所 | Method for degrading aflatoxin |
| CN102334624A (en) * | 2011-08-31 | 2012-02-01 | 青岛农业大学 | Device for degrading aflatoxin by using plasma and application of device |
-
2014
- 2014-11-17 CN CN201410651104.9A patent/CN105661114A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485408A (en) * | 2009-03-02 | 2009-07-22 | 中国农业科学院农产品加工研究所 | Method for degrading aflatoxin |
| CN102334624A (en) * | 2011-08-31 | 2012-02-01 | 青岛农业大学 | Device for degrading aflatoxin by using plasma and application of device |
Non-Patent Citations (2)
| Title |
|---|
| 张岩，等: ""等离子体降解黄曲霉毒素B1的试验研究"", 《中国粮油学报》 * |
| 李玉鹏，等: "低温射频等离子体降解农产品中黄曲霉毒素B1效果的研究", 《粮油食品科技》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110698534A (en) * | 2019-10-25 | 2020-01-17 | 郑州轻工业学院 | Method for improving functional characteristics of peanut meal protein and degrading aflatoxin |
| CN110698534B (en) * | 2019-10-25 | 2021-09-10 | 郑州轻工业大学 | Method for improving functional characteristics of peanut meal protein and degrading aflatoxin |
| CN110907241A (en) * | 2019-11-19 | 2020-03-24 | 上海市农业科学院 | Method for degrading deoxynivalenol in aqueous solution by using plasma |
| EP4406420A1 (en) | 2023-01-26 | 2024-07-31 | Jozef Stefan Institute | A method and a device for the degradation of aflatoxins using vacuum ultraviolet radiation |
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Application publication date: 20160615 |
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