CN105660402A - Method for saving Jiyichao chrysanthemum tissue culture seedling from mold contamination - Google Patents
Method for saving Jiyichao chrysanthemum tissue culture seedling from mold contamination Download PDFInfo
- Publication number
- CN105660402A CN105660402A CN201610080339.6A CN201610080339A CN105660402A CN 105660402 A CN105660402 A CN 105660402A CN 201610080339 A CN201610080339 A CN 201610080339A CN 105660402 A CN105660402 A CN 105660402A
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- CN
- China
- Prior art keywords
- bottle
- seedling
- chrysanthemum
- tissue culture
- chao
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
Abstract
The invention discloses a method for saving a Jiyichao chrysanthemum tissue culture seedling from mold contamination. By means of the method, contamination on the tissue culture seedling can be removed, the seedling can continue to grow, meanwhile, the rare seedling is saved, and losses are reduced. The method comprises the main steps of placing a Jiyichao chrysanthemum tissue culture contamination bottle on an ultra-clean workbench, then opening a bottle cap, pouring mercuric chloride into the bottle till the surface of a culture medium is immersed, conducting disinfection, finally directly cutting off the stem tip of the seedling with scissors, and transferring the stem tip into another culture medium bottle for culture.
Description
Technical field
The present invention is the processing method about tissue culture seedling pollution, in particular, is a kind of processing method saving discipline Yi Chao chrysanthemum tissue cultured seedling germ contamination.
Background technology
Plant tissue culture technique is one of key areas of modern biotechnology, be also be most widely used in agricultural production, the most successful field, play an important role in fields such as the preservation of plant elite germplasm, breeding, detoxification rejuvenation. Tissue culture technique is work organ, tissue or cell to be placed in culture medium, and be placed in suitable environment under sterile conditions, carries out the cell of cultivation, tissue or individuality continuously. Adopt plant tissue culture quick breeding technology, both can keep the feature of improved seeds, characteristic, the breeding of improved seeds can be accelerated again. In aseptic culture, always more or less having part young plant because a variety of causes causes that pollution causes tissue cultured seedling dead, the material causing part rare is lost. This method is aiming at polluting Seedling and takes corresponding disinfection way, at utmost saves and pollutes Seedling, reduces loss.
Summary of the invention
It is an object of the invention to overcome and pollute the death causing tissue cultured seedling, it is provided that a kind of technology processing group training pollution seedling-growing method, save the death of rare seedling, reduce loss.
The present invention is achieved in that
. a kind of method saving discipline Yi Chao chrysanthemum tissue cultured seedling germ contamination includes techniques below link:
1. pollution bottle is sterilized: discipline Yi Chao chrysanthemum group is trained pollution bottle and is placed on superclean bench, open bottle cap and 0.1% mercuric chloride is poured in bottle to flooding media surface disinfection;
2. inoculation: cut the stem apex of seedling in pollution bottle with shears on superclean bench, is inoculated in another medium bottle with tweezers by stem apex and cultivates, 1 stem apex of every bottle graft kind;
3. cultivate: the tissue culture bottle inoculated is put in group training room and cultivate, incubator temperature 20-25 DEG C, intensity of illumination 2000lx, light application time 12h/d.
Described mercuric chloride floods media surface height and is preferably 0.2-0.5cm, described in the time of disinfecting be preferably 5-8min.
Beneficial effects of the present invention:
The present invention, by disinfecting tissue culture's pollution bottle and Seedling, effectively eliminates the pollution of tissue cultured seedling so that seedling preserves cultivation preferably and gets off, and decreases loss.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is conducted further description;
Embodiment:
A kind of method saving discipline Yi Chao chrysanthemum tissue cultured seedling germ contamination, is characterized in that, it includes techniques below link:
1. pollution bottle is sterilized: discipline Yi Chao chrysanthemum group is trained pollution bottle and is placed on superclean bench, open bottle cap and 0.1% mercuric chloride is poured in bottle to flooding media surface 0.3cm, disinfect 6min;
2. inoculation: cut the stem apex of seedling in pollution bottle with shears on superclean bench, is inoculated in another medium bottle with tweezers by stem apex and cultivates, 1 stem apex of every bottle graft kind;
3. cultivate: the tissue culture bottle inoculated is put in group training room and cultivate, incubator temperature 20-25 DEG C, intensity of illumination 2000lx, light application time 12h/d.
Claims (3)
1. the method saving discipline Yi Chao chrysanthemum tissue cultured seedling germ contamination, is characterized in that, it includes techniques below link:
1. pollution bottle is sterilized: discipline Yi Chao chrysanthemum group is trained pollution bottle and is placed on superclean bench, open bottle cap and 0.1% mercuric chloride is poured in bottle to flooding media surface disinfection;
2. inoculation: cut the stem apex of seedling in pollution bottle with shears on superclean bench, is inoculated in another medium bottle with tweezers by stem apex and cultivates, 1 stem apex of every bottle graft kind;
3. cultivate: the tissue culture bottle inoculated is put in group training room and cultivate, incubator temperature 20-25 DEG C, intensity of illumination 2000lx, light application time 12h/d.
2. a kind of method saving discipline Yi Chao chrysanthemum tissue cultured seedling germ contamination according to claim 1, is characterized in that, it is 0.2-0.5cm that described mercuric chloride floods media surface.
3. a kind of method saving discipline Yi Chao chrysanthemum tissue cultured seedling germ contamination according to claim 1, is characterized in that, the described time of disinfecting is 5-8min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610080339.6A CN105660402A (en) | 2016-02-05 | 2016-02-05 | Method for saving Jiyichao chrysanthemum tissue culture seedling from mold contamination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610080339.6A CN105660402A (en) | 2016-02-05 | 2016-02-05 | Method for saving Jiyichao chrysanthemum tissue culture seedling from mold contamination |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105660402A true CN105660402A (en) | 2016-06-15 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610080339.6A Pending CN105660402A (en) | 2016-02-05 | 2016-02-05 | Method for saving Jiyichao chrysanthemum tissue culture seedling from mold contamination |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105660402A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598098A (en) * | 2013-11-19 | 2014-02-26 | 四川农业大学 | Rapid seedling culturing method for tissue culture of pachyrhizua angulatus |
| CN104396735A (en) * | 2014-09-22 | 2015-03-11 | 广西壮族自治区药用植物园 | Method for eliminating bacterial contamination in Momordica grosvenori tissue culture |
-
2016
- 2016-02-05 CN CN201610080339.6A patent/CN105660402A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598098A (en) * | 2013-11-19 | 2014-02-26 | 四川农业大学 | Rapid seedling culturing method for tissue culture of pachyrhizua angulatus |
| CN104396735A (en) * | 2014-09-22 | 2015-03-11 | 广西壮族自治区药用植物园 | Method for eliminating bacterial contamination in Momordica grosvenori tissue culture |
Non-Patent Citations (2)
| Title |
|---|
| 史绍林等: "山新杨组培苗污染后的处理与利用", 《防护林科技》 * |
| 熊丽等: "《观赏花卉的组织培养与大规模生产》", 31 January 2003 * |
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| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160615 |