CN105651652A - Oil spreading quantifying box - Google Patents
Oil spreading quantifying box Download PDFInfo
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- CN105651652A CN105651652A CN201610157877.0A CN201610157877A CN105651652A CN 105651652 A CN105651652 A CN 105651652A CN 201610157877 A CN201610157877 A CN 201610157877A CN 105651652 A CN105651652 A CN 105651652A
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- China
- Prior art keywords
- quantitative
- box
- dial plate
- hole
- biosurfactant
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N13/00—Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
- G01N13/02—Investigating surface tension of liquids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N13/00—Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
- G01N13/02—Investigating surface tension of liquids
- G01N2013/0275—Investigating surface tension of liquids involving surface-active agents
Abstract
The invention discloses an oil spreading quantifying box, comprising a quantifying box, a sample injection column, sealing plugs and a quantitative dial, wherein the quantifying box is a circular transparent box body; the sample injection column is a solid glass column; the lower end of the sample injection column is fixed in the middle of the bottom in the quantifying box, and a 3cm of gap is reserved between the upper end of the sample injection column and a cover body of the quantifying box; a sample injection hole is formed in the middle of the cover body of the quantifying box and is positioned above the sample injection column; a material injection hole is formed in one side of the cover body of the quantifying box; one of the two sealing plugs is connected with the sample injection hole, and the other sealing plug is connected with the material injection hole; the quantitative dial is made of transparent material; the quantitative dial is provided with ten equally divided rings which are marked with numerical symbols. Compared with the prior art, the oil spreading quantifying box is capable of conveniently, rapidly, accurately and stably measuring the content of a biological surfactant; after the oil spreading quantifying box is used, the quantitation standards for different types of biological surfactants or one biological surfactant in different environments can be established; the oil spreading quantifying box is convenient to use and has popularization and application values.
Description
Technical field
The present invention relates to the device of a kind of quantitative biosurfactant class material, particularly relate to a kind of oil extraction delineation amount box.
Background technology
When biosurfactant is microorganism or plant cultivates under certain condition, the oozy metabolite with certain surface activity in its metabolic process. The molecular structure of biosurfactant is mainly made up of two parts: a part is the polar group that oleophobic is hydrophilic, the non-polar group that another part is made up of the hydrocarbon chain of hydrophobic oleophilic oil. The kind of biosurfactant include the glycolipid synthesized by pseudomonas, the lipopeptid synthesized by bacillus cereus, by the phospholipid of other Microbe synthesis, lipopolysaccharide or neutral fat derivant etc. Biosurfactant has good solubilising, significantly reduces the feature such as surface tension, stable emulsion. Additionally, also have biodegradable, low toxicity or nontoxic, the not advantage such as sensitization. Compared with synthetic surfactant, biosurfactant has unique environmentally friendly characteristic. Therefore, biosurfactant obtains relatively broad application at each industrial circle, particularly in environment remediation and field of petroleum exploitation, such as microbe oil production, repairs the environment etc. that crude oil leakage pollutes.
In recent years, the development and application attention of biosurfactant, the fast quantification of Bio-surface active material in separation screening biosurfactant production microorganism and monitoring microbe survival environment in metabolite biosurfactant content there is extremely important meaning. At present, the quantitative approach of biosurfactant mainly has blood plate method, oil droplet to subside method and oil spreading. Wherein, blood plate method error is relatively big, and oil droplet subsides method complex operation. Comparatively speaking, oil spreading is simple to operate, and universality is strong. Tradition oil extraction circle method is to add a certain amount of distilled water and oil film material in culture dish, for instance the dodecane hydrocarbon that dyeed by Sudan red III, paraffin etc. Oil film material, after distilled water surface spreading is uniform, drips a certain amount of biosurfactant solution at oil film center, relies on it to reduce capillary characteristic and forms an oil extraction circle in oil film centre. Then the content of biosurfactant is judged according to oil extraction loop diameter size.
Tradition oil spreading is primarily present following problem: 1. culture dish is open, and the impact by surrounding is bigger.When surrounding is more complicated, than if any relatively air blast or variation of ambient temperature bigger time, the oil extraction circle of formation is unstable and difference in size is bigger; 2. the more difficult Quick uniform of oil film is sprawled, and makes experimental repeatability and accuracy there is limitation. The oil film free spreading rate on distilled water surface is relatively slow, and waiting oil film to sprawl completely needs to consume the longer time. When the oil film material volume added is identical, the oil film size every time formed is also inconsistent, illustrates that the thickness of oil film is different, and the oil extraction circle size that the oil film Surfactant of different-thickness generates has obvious impact. ; 3. oil extraction circle is shorter for stabilization time, often observes and inaccurate even cannot observe. Observing immediately after adding biosurfactant solution, the oil extraction circle formed is also unstable, the actual content of the very difficult size accurate response biosurfactant by oil extraction circle. It addition, the oil extraction circle formed is very short for stabilization time, oil extraction circle is easy to break oil film and cause and cannot observe for experimental result.
Additionally, Bio-surface active material is often the mixture of two kinds or more of biosurfactant or the multiple mixture with surfactant to have to be concerned with in prior art, traditional method all cannot realize the mensuration of its total content, therefore cannot provide its surface-active ability.
Summary of the invention
The purpose of the present invention is that provides a kind of oil extraction delineation amount box to solve the problems referred to above.
The present invention is achieved through the following technical solutions above-mentioned purpose:
The present invention includes quantitative box, note sample post, sealing-plug and quantitative dial plate, described quantitative box is circular non-opaque box body, described note sample post is solid glass post, the centre of described quantitative box inner bottom part is fixed in the lower end of described note sample post, the upper end of described note sample post has the gap of 3cm from the lid of described quantitative box, note sample hole it is provided with in the middle part of the lid of described quantitative box, described note sample hole is positioned at the top of described note sample post, the lid side of described quantitative box is provided with material hole, described sealing-plug is two, one described sealing-plug is connected with described note sample hole, another described sealing-plug is connected with described material hole, described quantitative dial plate is transparent material, described quantitative dial plate is provided with ten annulars of decile and is marked with numeral labelling.
Further, the annulet manufacture method of described quantitative dial plate is:
A prepares a certain biosurfactant standard solution of variable concentrations gradient;
B injects distilled water and the oil film material of certain volume in quantitative box, circular hole is sealed, acutely concussion, oil film material is static by it after being uniformly dispersed, uniformly spread in after distilled water upper surface until oil film material, utilize the pipettor standard solution noting a certain concentration being uniformly injected into certain volume on sample post to quantitative box center;
The oil extraction circle of formation, after it is stable, by quantitative dial plate as quantitative box upper surface, is compared with the graduation mark on quantitative dial plate, and degree line that time of coincidence is put on corresponding concentration numbers word mark by c;
D repeat the above steps, and the concentration scale on scale dial plate is supplemented complete.
Further, described oil extraction delineation amount box comprises the following steps for the quantitative approach of biosurfactant sample:
A injects distilled water and the oil film material of certain volume in quantitative box, will note sample hole and material hole seals, and acutely shake, and oil film material treats after being uniformly dispersed that it is static;
B uniformly spreads in after distilled water upper surface until oil film material, pipettor is utilized to be uniformly injected into a certain biosurfactant sample of certain volume on the note sample post at quantitative box center, after it is stable, by quantitative dial plate as quantitative box upper surface, the oil extraction circle of formation is compared with the graduation mark on quantitative dial plate, quantitative dial plate directly reads the content of biosurfactant in sample.
The beneficial effects of the present invention is:
The present invention is a kind of oil extraction delineation amount box, compared with prior art, the present invention can be convenient, fast, accurate, stable measure biosurfactant content, and use the present invention can set up under different types of biosurfactant or varying environment the quantitative criterion for biosurfactant, easy to use, there is the value of popularization and application.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention;
Fig. 2 is the quantitative index dial structure schematic diagram of the present invention.
In figure: the quantitative box of 1-, 2-note sample post, 3-notes sample hole, 4-sealing-plug, 5-material hole, the quantitative dial plate of 6-.
Detailed description of the invention
Below in conjunction with accompanying drawing, the invention will be further described:
As depicted in figs. 1 and 2: the present invention includes quantitative box 1, note sample post 2, sealing-plug 4 and quantitative dial plate 6, described quantitative box 1 is circular non-opaque box body, described note sample post 2 is solid glass post, the centre of described quantitative box 1 inner bottom part is fixed in the lower end of described note sample post 2, the upper end of described note sample post 2 has the gap of 3cm from the lid of described quantitative box 1, note sample hole 3 it is provided with in the middle part of the lid of described quantitative box 1, described note sample hole 3 is positioned at the top of described note sample post 2, the lid side of described quantitative box 1 is provided with material hole 5, described sealing-plug 4 is two, one described sealing-plug 4 is connected with described note sample hole 3, another described sealing-plug 4 is connected with described material hole 5, described quantitative dial plate 6 is transparent material, described quantitative dial plate 6 is provided with ten annulars of decile and is marked with numeral labelling.
Further, the annulet manufacture method of described quantitative dial plate 6 is:
A prepares a certain biosurfactant standard solution of variable concentrations gradient;
B injects distilled water and the oil film material of certain volume in quantitative box 1, circular hole is sealed, acutely concussion, oil film material is static by it after being uniformly dispersed, uniformly spread in after distilled water upper surface until oil film material, utilize the pipettor standard solution noting a certain concentration being uniformly injected into certain volume on sample post 2 to quantitative box 1 center;
The oil extraction circle of formation, after it is stable, by quantitative dial plate 6 as quantitative box 1 upper surface, is compared with the graduation mark on quantitative dial plate 6, and degree line that time of coincidence is put on corresponding concentration numbers word mark by c;
D repeat the above steps, and the concentration scale on scale dial plate is supplemented complete.
Further, described oil extraction delineation amount box 1 comprises the following steps for the quantitative approach of biosurfactant sample:
A injects distilled water and the oil film material of certain volume in quantitative box 1, is sealed in note sample hole 3 and material hole 5, acutely shakes, and oil film material treats that it is static after being uniformly dispersed;
B uniformly spreads in after distilled water upper surface until oil film material, pipettor is utilized to be uniformly injected into a certain biosurfactant sample of certain volume on the note sample post 2 at quantitative box 1 center, after it is stable, by quantitative dial plate 6 as quantitative box 1 upper surface, the oil extraction circle of formation is compared with the graduation mark on quantitative dial plate 6, quantitative dial plate 6 directly reads the content of biosurfactant in sample.
The present invention includes quantitative box 1 and 6 two parts of quantitative dial plate, and quantitative box 1 is semiclosed glass device, is the interference in order to get rid of extraneous complex environment to greatest extent when carrying out the experiment of oil extraction circle, so that the oil extraction circle stable existence formed; Note sample post 2 is solid glass post, it is possible to the oil extraction circle of formation is fixed on the center of quantitative box 1, and namely oil extraction circle with solid glass post for center of circle stable existence in quantitative box 1, and will can improve the stabilization time of oil extraction circle.It addition, when injecting sample, make sample slowly note sample post 2 wall uniformly and stay, it is possible to avoid sample to add the too fast oil extraction circle splintering problem forcing surface tension of oil membrane to be suddenlyd change and to cause; Shake quantitative box 1, after making oil film material mix homogeneously with distilled water it is static, shorten oil film material with this and sprawl the time on distilled water surface, and the oil film material of the same volume degree of sprawling in quantitative box 1 can be made suitable, thus solve oil film freely sprawl time length and oil film sprawls problem in uneven thickness afterwards; The effect of quantitative dial plate 6 is to provide quantitative criterion for biosurfactant content under quantitative a certain type biological surfactant or varying environment, the drafting of standard curve is replaced with this quantitative dial plate 6, the standard concentration recorded is marked in quantitative dial plate 6, and then directly measure the content of biosurfactant in sample as scale, thus eliminate data to calculate this step.
The ultimate principle of the present invention and principal character and advantages of the present invention have more than been shown and described. Skilled person will appreciate that of the industry; the present invention is not restricted to the described embodiments; described in above-described embodiment and description is that principles of the invention is described; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements both fall within the claimed scope of the invention. Claimed scope is defined by appending claims and equivalent thereof.
Claims (3)
1. an oil extraction delineation amount box, it is characterized in that: include quantitative box, note sample post, sealing-plug and quantitative dial plate, described quantitative box is circular non-opaque box body, described note sample post is solid glass post, the centre of described quantitative box inner bottom part is fixed in the lower end of described note sample post, the upper end of described note sample post has the gap of 3cm from the lid of described quantitative box, note sample hole it is provided with in the middle part of the lid of described quantitative box, described note sample hole is positioned at the top of described note sample post, the lid side of described quantitative box is provided with material hole, described sealing-plug is two, one described sealing-plug is connected with described note sample hole, another described sealing-plug is connected with described material hole, described quantitative dial plate is transparent material, described quantitative dial plate is provided with ten annulars of decile and is marked with numeral labelling.
2. oil extraction delineation amount box according to claim 1, it is characterised in that: the annulet manufacture method of described quantitative dial plate is:
A prepares a certain biosurfactant standard solution of variable concentrations gradient;
B injects distilled water and the oil film material of certain volume in quantitative box, circular hole is sealed, acutely concussion, oil film material is static by it after being uniformly dispersed, uniformly spread in after distilled water upper surface until oil film material, utilize the pipettor standard solution noting a certain concentration being uniformly injected into certain volume on sample post to quantitative box center;
The oil extraction circle of formation, after it is stable, by quantitative dial plate as quantitative box upper surface, is compared with the graduation mark on quantitative dial plate, and degree line that time of coincidence is put on corresponding concentration numbers word mark by c;
D repeat the above steps, and the concentration scale on scale dial plate is supplemented complete.
3. oil extraction delineation amount box according to claim 1, it is characterised in that: described oil extraction delineation amount box comprises the following steps for the quantitative approach of biosurfactant sample:
A injects distilled water and the oil film material of certain volume in quantitative box, will note sample hole and material hole seals, and acutely shake, and oil film material treats after being uniformly dispersed that it is static;
B uniformly spreads in after distilled water upper surface until oil film material, pipettor is utilized to be uniformly injected into a certain biosurfactant sample of certain volume on the note sample post at quantitative box center, after it is stable, by quantitative dial plate as quantitative box upper surface, the oil extraction circle of formation is compared with the graduation mark on quantitative dial plate, quantitative dial plate directly reads the content of biosurfactant in sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610157877.0A CN105651652B (en) | 2016-03-18 | 2016-03-18 | Oil extraction delineation amount box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610157877.0A CN105651652B (en) | 2016-03-18 | 2016-03-18 | Oil extraction delineation amount box |
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| Publication Number | Publication Date |
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| CN105651652A true CN105651652A (en) | 2016-06-08 |
| CN105651652B CN105651652B (en) | 2018-08-28 |
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| CN201610157877.0A Active CN105651652B (en) | 2016-03-18 | 2016-03-18 | Oil extraction delineation amount box |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2070282C1 (en) * | 1992-02-21 | 1996-12-10 | Татарский научно-исследовательский и проектный институт нефтяной промышленности | Method for development of oil formation |
| CN101928686A (en) * | 2010-05-20 | 2010-12-29 | 黑龙江大学 | Bacillus subtilis secreting surfactant |
| CN103160452A (en) * | 2013-01-27 | 2013-06-19 | 盐城师范学院 | Method capable of selecting and screening surfactant hydrocarbon degradation bacteria on surface of forming material |
| CN103940706A (en) * | 2014-05-08 | 2014-07-23 | 中国石油天然气股份有限公司 | Quantitative analysis method for interfacial tension performance of petroleum crude oil ternary compound system and determination method of ternary compound system |
| CN104372064A (en) * | 2014-10-21 | 2015-02-25 | 中国石油化工股份有限公司 | Rapid high-throughput screening method for oil-recovery microorganisms |
| CN205620268U (en) * | 2016-03-18 | 2016-10-05 | 中国海洋大学 | Arrange oil ring ration box |
-
2016
- 2016-03-18 CN CN201610157877.0A patent/CN105651652B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2070282C1 (en) * | 1992-02-21 | 1996-12-10 | Татарский научно-исследовательский и проектный институт нефтяной промышленности | Method for development of oil formation |
| CN101928686A (en) * | 2010-05-20 | 2010-12-29 | 黑龙江大学 | Bacillus subtilis secreting surfactant |
| CN103160452A (en) * | 2013-01-27 | 2013-06-19 | 盐城师范学院 | Method capable of selecting and screening surfactant hydrocarbon degradation bacteria on surface of forming material |
| CN103940706A (en) * | 2014-05-08 | 2014-07-23 | 中国石油天然气股份有限公司 | Quantitative analysis method for interfacial tension performance of petroleum crude oil ternary compound system and determination method of ternary compound system |
| CN104372064A (en) * | 2014-10-21 | 2015-02-25 | 中国石油化工股份有限公司 | Rapid high-throughput screening method for oil-recovery microorganisms |
| CN205620268U (en) * | 2016-03-18 | 2016-10-05 | 中国海洋大学 | Arrange oil ring ration box |
Non-Patent Citations (3)
| Title |
|---|
| MASAAKI MORIKAWA ETC.: "A study on the structure^function relationship of lipopeptide biosurfactants", 《BIOCHIMICA ET BIOPHYSICA ACTA》 * |
| 张凡 等: ""排油圈法对生物表面活性剂的定性与定量"", 《化学工程师》 * |
| 张飞龙 等: ""生物表面活性剂高产菌用排油圈法初筛时的影响因素分析"", 《化学工程师》 * |
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| CN105651652B (en) | 2018-08-28 |
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