CN105648015A - Extraction method of Rhodiola rosea entity small molecule peptide - Google Patents
Extraction method of Rhodiola rosea entity small molecule peptide Download PDFInfo
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- CN105648015A CN105648015A CN201610190964.6A CN201610190964A CN105648015A CN 105648015 A CN105648015 A CN 105648015A CN 201610190964 A CN201610190964 A CN 201610190964A CN 105648015 A CN105648015 A CN 105648015A
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- radix rhodiolae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
Abstract
The invention discloses an extraction method of Rhodiola rosea entity small molecule peptide. The extraction method includes following steps: drying Rhodiola rosea, and smashing the same into Rhodiola rosea powder; mixing the Rhodiola rosea powder with water to form mixed liquid, heating the mixed liquid, holding temperature for a certain period of time, and cooling; adding alkaline protease into the mixed liquid after being cooled, adjusting pH, heating the mixed liquid, and holding temperature for a certain period of time to obtain an alkaline solution; adding neutral protease into the alkaline solution, adjusting pH, heating, and holding temperature for a certain period of time to obtain a neutral solution; adding pepsase into the neutral solution, adjusting pH, heating, and holding temperature to obtain an acidic solution; heating the acidic solution, holding temperature for a certain period of time, and filtering to obtain filtrate; decoloring the filtrate to obtain clear liquid, concentrating the clear liquid, and drying to obtain Rhodiola rosea entity small molecule peptide powder. The extraction method has the advantage that the small molecule peptide extracted from Rhodiola rosea entity has efficacy of improving immunity, neurological function and memory if being eaten frequently.
Description
Technical field
The present invention relates to a kind of extracting method, be specifically related to the extracting method of a kind of Radix Rhodiolae entity small-molecular peptides.
Background technology
Small-molecular peptides is a kind of classification of polypeptide, molecular weight section is typically in below 1000 dalton, also referred to as little peptide, oligopeptide or small active peptides, generally it is made up of 2-6 aminoacid, it has and need not digest, directly absorb, body energy need not be consumed during absorption, human intestines and stomach's function burden and the advantage that absorption of human body can be forced when absorption of human body afunction will not be increased, in addition it also has the function of carrier, can other nutrition that people eats be loaded on its body, it is transported to the cell of human body, tissue, various trace element are transported each position of human body.
But current naturally occurring peptide is little, producing of present small-molecular peptides is mainly obtained by Proteolytic enzyme, proteoclastic method mainly has acid system, alkaline process and enzyme process, the shortcomings such as wherein acid, alkaline process has cost height, complex process, hydrolysis degree is wayward, environmental pollution is serious, and when high-alkali or peracid, albumen and the impurity such as sugar and oils and fats react, and produce brown material and even produce harmful substance, and they can affect the quality of small-molecular peptides. The hydrolysis of enzyme is gentleer, occurs without bad side reaction, but the unicity of enzyme effect makes to be extremely difficult to desirable hydrolysis effect by a kind of enzyme, and protein extracting ratio is low.
In recent years, the using value of small-molecular peptides is increasingly subject to people's attention, its correlational study also more to be had been reported that, such as the Chinese patent CN103409491A preparation method disclosing a kind of rice protein small-molecular peptides, it comprises the following steps: pretreatment of raw material, pulverizing, tune oar, wash, size mixing, react, concentrate, the step such as dehydration. And the extraction about Radix Rhodiolae entity small-molecular peptides also rarely has report.
Summary of the invention
It is an object of the invention to provide the extracting method of a kind of Radix Rhodiolae entity small-molecular peptides.
It is an object of the invention to be achieved through the following technical solutions:
The extracting method of a kind of Radix Rhodiolae entity small-molecular peptides, comprises the following steps,
(1) take Radix Rhodiolae to be dried, more dried Radix Rhodiolae is ground into Radix Rhodiolae powder;
(2) Radix Rhodiolae powder being uniformly mixed into mixed liquor with water, heating mixed liquor is to after 90-100 DEG C, being incubated a period of time, then cool down;
(3) in the mixed liquor after cooling, add alkaline protease, and regulate pH to 8-10, then heating mixed liquor to 45-55 DEG C, be incubated a period of time, obtain alkaline solution;
(4) in alkaline solution, add neutral protease, and regulate pH to 6.8-7.0, be then heated to 45-55 DEG C, be incubated a period of time, obtain neutral solution;
(5) in neutral solution, add pepsin, and regulate pH to 3.5-3.7, be then heated to 45-55 DEG C, be incubated a period of time, obtain acid solution;
(6) acid solution is heated to 90-100 DEG C, be incubated a period of time, filter to get filtrate;
(7) filtrate carrying out desolventing technology and obtains supernatant liquid, concentrating clarifying liquid also dries to obtain Radix Rhodiolae entity small-molecular peptides powder.
Further, dry described in step (1) as by Radix Rhodiolae dry 15-18h at the temperature of 50-60 DEG C.
Further, described in step (1), the fineness of Radix Rhodiolae powder is 80-120 order.
Further, in step (2), the weight ratio of Radix Rhodiolae and water is 1:12.5, mixing and stirring when being 60-70r/min by Radix Rhodiolae and water low whipping speed; The heating rate of described heating is 20-30 DEG C/min; The time of described insulation is 10-15min; Described cooling is for being cooled to 45-55 DEG C.
Further, described in step (3), the vigor of alkaline protease is 500,000 u/g, and the weight ratio of the weight of described alkaline protease and Radix Rhodiolae powder is 1:100; The sodium hydrate aqueous solution using mass fraction to be 10% in step (3) regulates pH; Temperature retention time described in step (3) is 3-3.5h. Alkaline protease refers in the basic conditions can the enzyme of aminosal peptide bond, it can generate polypeptide or aminoacid by aminosal molecule peptide chain, the pH that alkaline protease is suitable for ranges for 8-10, suitable temperature range is 50-60 DEG C, the time of reaction is probably at 3-6 hour, so to regulate pH after alkaline protease adding and heat so that it is reach to be suitable for pH and the temperature of alkaline protease, and the temperature survived at applicable alkaline protease and pH conditioned response 3-3.5h.
Further, the vigor of described neutral protease is 150,000 u/g, and the weight ratio of the weight of described neutral protease and Radix Rhodiolae powder is 1:100; The aqueous hydrochloric acid solution using mass fraction to be 10% in step (4) regulates pH; Temperature retention time described in step (4) is 3-3.5h. Neutral protease is obtained by the fermented extraction of bacillus subtilis, belongs to a kind of restriction endonuclease, can be used for various proteolysis and processes, and suitable temperature range is 45-50 DEG C, suitable pH ranges for 6.8-7.0. It can be hydrolyzed into vegeto-animal macro-molecular protein small-molecular peptides or aminoacid, is beneficial to effectively absorbing and utilizing of protein, and degree of hydrolysis is high, local flavor is good.
Further, pepsic vigor described in step (5) is 50,000 u/g, and the weight ratio of described pepsic weight and Radix Rhodiolae powder is 1:100; The aqueous hydrochloric acid solution using mass fraction to be 10% in step (5) regulates pH; Temperature retention time described in step (5) is 3-3.5h.Pepsin is a kind of digestible protein enzyme, and secreted by the gastric mucosa chief cell in stomach, function is to be little fragments of peptides by the breaks down proteins in food.
Further, described in step (6), the time of insulation is 15-20min; Described being filtered into is filtered with the defecator of 300 order-500 orders. It is incubated 15-20min when 90-100 DEG C in order to enzyme is carried out inactivation treatment, is filtered with the Coarse Mesh Gauze of 300-500 order or filter paper after enzyme is carried out inactivation treatment, obtains filtrate.
Further, adding the activated carbon accounting for described filtrate weight 2%-3% in step (7) in filtrate, at the temperature of 65-75 DEG C, stirring decolouring 25-35min, then filters to obtain supernatant liquid; Concentrating clarifying liquid is to its former ponderable 15%-25%, then is dried to obtain Radix Rhodiolae entity small-molecular peptides powder. Activated carbon is the solid carbonaceous of a kind of black porous, is mainly composed of carbon and containing elements such as a small amount of oxygen, hydrogen, sulfur, nitrogen chlorine, has very strong absorption property, for a kind of industrial adsorbents that purposes is extremely wide. During concentration, supernatant liquid is heated to about 60 DEG C and adopts vacuum concentration, time dry, adopt spray drying or lyophilization.
The invention have the benefit that the extracting method of Radix Rhodiolae entity small-molecular peptides of the present invention utilizes compound bio enzyme resolving tech, and by regulating enzymolysis parameter, farthest play the effect of various enzyme preparation, thus greatly reducing the consumption of enzyme preparation, save production cost, and the molecular weight of products obtained therefrom is mainly distributed between 100-700D, the small-molecular peptides of gained mostly be dipeptides, tripeptides, tetrapeptide etc. very easily be absorbed by the body utilize small molecule structure, molecular weight distribution accounts for more than the 90% of Tot Prot in the small-molecular peptides of 100-700D;
Often the small-molecular peptides of edible Radix Rhodiolae entity extraction of the present invention has raising immunity, improves effect of function of nervous system, memory reinforcing, and there is no any known side effect, too much absorption can be discharged with urine and bile rapidly, therefore can be widely used in medical material and health food.
Detailed description of the invention
Embodiment one
The extracting method of a kind of Radix Rhodiolae entity small-molecular peptides, comprises the following steps:
(1) by Radix Rhodiolae dry 15h at the temperature of 50 DEG C, dried Radix Rhodiolae is ground into the Radix Rhodiolae powder that fineness of the particles is 80 orders; Radix Rhodiolae is pulverized, is conducive to being sufficiently mixed of Radix Rhodiolae and water, it is simple to the precipitation of effective ingredient in Radix Rhodiolae;
(2) when being 60r/min by the Radix Rhodiolae that weight ratio is 1:12.5 with water low whipping speed, mixing and stirring becomes mixed liquor, then 90 DEG C it are heated to, and after being incubated 10min, it is cooled to 45 DEG C, keeping, when heating, the speed that temperature raises is 20 DEG C/min, because being conducive to the precipitation of effective ingredient in Radix Rhodiolae when heating temperatures to 90 DEG C;
(3) toward cooling after mixed liquor in addition vigor be 500,000 u/g alkaline proteases, the added weight of alkaline protease is 1:100 with the weight ratio of Radix Rhodiolae powder, the pH being suitable for because of alkaline protease ranges for 8-11, so after adding alkaline protease, pH to 8 need to be regulated, then heating mixed liquor to 45 DEG C, insulation 3h obtains alkaline solution; As the preferred scheme of one, when regulating pH operating weight mark be 10% sodium hydrate aqueous solution be adjusted;
(4) toward addition vigor in alkaline solution be the neutral protease of 150,000 u/g, the added weight of neutral protease and the weight ratio of Radix Rhodiolae powder are 1:100, the pH suitable because of neutral protease ranges for 6.8-7.0, so needing to regulate pH to 6.8 after adding neutral protease, it is then heated to 45 DEG C, the insulation 3h time, obtain neutral solution;As the preferred scheme of one, when regulating pH operating weight mark be 10% aqueous hydrochloric acid solution be adjusted;
(5) being the pepsin of 50,000 u/g toward addition vigor in neutral solution, the weight ratio of added pepsic weight and Radix Rhodiolae powder is 1:100, then regulates pH to 3.5, and heats to 45 DEG C, is incubated 3h, obtains acid solution; As the preferred scheme of one, the aqueous hydrochloric acid solution using mass fraction to be 10% regulates pH;
(6) heating acid solution to 90 DEG C, enzyme is carried out inactivation treatment by insulation 15min, is then filtered obtaining filtrate with the filter paper of 300 orders;
(7) filtrate carrying out desolventing technology and obtains supernatant liquid, concentrating clarifying liquid also dries to obtain Radix Rhodiolae entity small-molecular peptides powder. Adding in filtrate and account for described filtrate weight 2% activated carbon, at the temperature of 65 DEG C, stirring decolouring 25min, then filters to obtain supernatant liquid; Supernatant liquid is heated to 60 DEG C and adopts vacuum concentration, supernatant liquid is concentrated into its former ponderable 15%, then adopt spray drying to obtain Radix Rhodiolae entity small-molecular peptides powder.
Embodiment two
The extracting method of a kind of Radix Rhodiolae entity small-molecular peptides, comprises the following steps:
(1) by Radix Rhodiolae dry 16h at the temperature of 55 DEG C, dried Radix Rhodiolae is ground into the Radix Rhodiolae powder that fineness of the particles is 100 orders; Radix Rhodiolae is pulverized, is conducive to being sufficiently mixed of Radix Rhodiolae and water, it is simple to the precipitation of effective ingredient in Radix Rhodiolae;
(2) when being 65r/min by the Radix Rhodiolae that weight ratio is 1:12.5 with water low whipping speed, mixing and stirring becomes mixed liquor, then 95 DEG C it are heated to, and after being incubated 12min, it is cooled to 50 DEG C, keeping, when heating, the speed that temperature raises is 25 DEG C/min, because being conducive to the precipitation of effective ingredient in Radix Rhodiolae when heating temperatures to 95 DEG C;
(3) toward cooling after mixed liquor in addition vigor be 500,000 u/g alkaline proteases, the added weight of alkaline protease is 1:100 with the weight ratio of Radix Rhodiolae powder, the pH being suitable for because of alkaline protease ranges for 8-11, so after adding alkaline protease, pH to 9 need to be regulated, then heating mixed liquor to 50 DEG C, insulation 3.2h obtains alkaline solution; As the preferred scheme of one, when regulating pH operating weight mark be 10% sodium hydrate aqueous solution be adjusted;
(4) toward addition vigor in alkaline solution be the neutral protease of 150,000 u/g, the added weight of neutral protease and the weight ratio of Radix Rhodiolae powder are 1:100, the pH suitable because of neutral protease ranges for 6.8-7.0, so needing to regulate pH to 6.8 after adding neutral protease, it is then heated to 50 DEG C, insulation 3.2h, obtains neutral solution; As the preferred scheme of one, when regulating pH operating weight mark be 10% aqueous hydrochloric acid solution be adjusted;
(5) being the pepsin of 50,000 u/g toward addition vigor in neutral solution, the weight ratio of added pepsic weight and Radix Rhodiolae powder is 1:100, then regulates pH to 3.6, and heats to 50 DEG C, is incubated 3.2h, obtains acid solution; As the preferred scheme of one, the aqueous hydrochloric acid solution using mass fraction to be 10% regulates pH;
(6) heating acid solution to 95 DEG C, enzyme is carried out inactivation treatment by insulation 18min, is then filtered obtaining filtrate with the Coarse Mesh Gauze of 400 orders;
(7) filtrate carrying out desolventing technology and obtains supernatant liquid, concentrating clarifying liquid also dries to obtain Radix Rhodiolae entity small-molecular peptides powder. Adding the activated carbon accounting for described filtrate weight 2.5% in filtrate, at the temperature of 70 DEG C, stirring decolouring 30min, then filters to obtain supernatant liquid;Supernatant liquid is heated to 60 DEG C and adopts vacuum concentration, supernatant liquid is concentrated into its former ponderable 20%, then adopt lyophilization to obtain Radix Rhodiolae entity small-molecular peptides powder.
Embodiment three
The extracting method of a kind of Radix Rhodiolae entity small-molecular peptides, comprises the following steps:
(1) by Radix Rhodiolae dry 18h at the temperature of 60 DEG C, dried Radix Rhodiolae is ground into the Radix Rhodiolae powder that fineness of the particles is 120 orders; Radix Rhodiolae is pulverized, is conducive to being sufficiently mixed of Radix Rhodiolae and water, it is simple to the precipitation of effective ingredient in Radix Rhodiolae;
(2) when being 70r/min by the Radix Rhodiolae that weight ratio is 1:12.5 with water low whipping speed, mixing and stirring becomes mixed liquor, then 100 DEG C it are heated to, and after being incubated 15min, it is cooled to 55 DEG C, keeping, when heating, the speed that temperature raises is 30 DEG C/min, because being conducive to the precipitation of effective ingredient in Radix Rhodiolae when heating temperatures to 100 DEG C;
(3) toward cooling after mixed liquor in addition vigor be 500,000 u/g alkaline proteases, the added weight of alkaline protease is 1:100 with the weight ratio of Radix Rhodiolae powder, the pH being suitable for because of alkaline protease ranges for 8-11, so after adding alkaline protease, pH to 10 need to be regulated, then heating mixed liquor to 55 DEG C, insulation 3.5h obtains alkaline solution; As the preferred scheme of one, when regulating pH operating weight mark be 10% sodium hydrate aqueous solution be adjusted;
(4) toward addition vigor in alkaline solution be the neutral protease of 150,000 u/g, the added weight of neutral protease and the weight ratio of Radix Rhodiolae powder are 1:100, the pH suitable because of neutral protease ranges for 6.8-7.0, so needing to regulate pH to 7.0 after adding neutral protease, it is then heated to 55 DEG C, the insulation 3.5h time, obtain neutral solution; As the preferred scheme of one, when regulating pH operating weight mark be 10% aqueous hydrochloric acid solution be adjusted;
(5) being the pepsin of 50,000 u/g toward addition vigor in neutral solution, the weight ratio of added pepsic weight and Radix Rhodiolae powder is 1:100, then regulates pH to 3.7, and heats to 55 DEG C, is incubated 3.5h, obtains acid solution; As the preferred scheme of one, the aqueous hydrochloric acid solution using mass fraction to be 10% regulates pH;
(6) heating acid solution to 100 DEG C, enzyme is carried out inactivation treatment by insulation 20min, is then filtered obtaining filtrate with the Coarse Mesh Gauze of 500 orders;
(7) filtrate carrying out desolventing technology and obtains supernatant liquid, concentrating clarifying liquid also dries to obtain Radix Rhodiolae entity small-molecular peptides powder. Adding the activated carbon accounting for described filtrate weight 3% in filtrate, at the temperature of 75 DEG C, stirring decolouring 35min, then filters to obtain supernatant liquid; Supernatant liquid is heated to 60 DEG C and adopts vacuum concentration, supernatant liquid is concentrated into its former ponderable 25%, then adopt spray drying to obtain Radix Rhodiolae entity small-molecular peptides powder.
The present invention is not limited to above-mentioned preferred forms; anyone can draw other various forms of products under the enlightenment of the present invention; no matter but in its details, do any change, every have same or like with the application like technical scheme, all fall within protection scope of the present invention.
Claims (9)
1. the extracting method of a Radix Rhodiolae entity small-molecular peptides, it is characterised in that comprise the following steps:
(1) take Radix Rhodiolae to be dried, more dried Radix Rhodiolae is ground into Radix Rhodiolae powder;
(2) Radix Rhodiolae powder being uniformly mixed into mixed liquor with water, heating mixed liquor is to after 90-100 DEG C, being incubated a period of time, then cool down;
(3) in the mixed liquor after cooling, add alkaline protease, and regulate pH to 8-10, then heating mixed liquor to 45-55 DEG C, be incubated a period of time, obtain alkaline solution;
(4) in alkaline solution, add neutral protease, and regulate pH to 6.8-7.0, be then heated to 45-55 DEG C, be incubated a period of time, obtain neutral solution;
(5) in neutral solution, add pepsin, and regulate pH to 3.5-3.7, be then heated to 45-55 DEG C, be incubated a period of time, obtain acid solution;
(6) acid solution is heated to 90-100 DEG C, be incubated a period of time, filter to get filtrate;
(7) filtrate carrying out desolventing technology and obtains supernatant liquid, concentrating clarifying liquid also dries to obtain Radix Rhodiolae entity small-molecular peptides powder.
2. the extracting method of Radix Rhodiolae entity small-molecular peptides according to claim 1, it is characterised in that dry described in step (1) as by Radix Rhodiolae dry 15-18h at the temperature of 50-60 DEG C.
3. the extracting method of Radix Rhodiolae entity small-molecular peptides according to claim 1, it is characterised in that described in step (1), the fineness of Radix Rhodiolae powder is 80-120 order.
4. the extracting method of Radix Rhodiolae entity small-molecular peptides according to claim 1, it is characterized in that, in step (2), the weight ratio of Radix Rhodiolae and water is 1:12.5, mixing and stirring when being 60-70r/min by Radix Rhodiolae and water low whipping speed; The heating rate of described heating is 20-30 DEG C/min; The time of described insulation is 10-15min; Described cooling is for being cooled to 45-55 DEG C.
5. the weight ratio of the extracting method of Radix Rhodiolae entity small-molecular peptides according to claim 1, it is characterised in that described in step (3), the vigor of alkaline protease is 500,000 u/g, the weight of described alkaline protease and Radix Rhodiolae powder is 1:100; The sodium hydrate aqueous solution using mass fraction to be 10% in step (3) regulates pH; Temperature retention time described in step (3) is 3-3.5h.
6. the weight ratio of the extracting method of Radix Rhodiolae entity small-molecular peptides according to claim 1, it is characterised in that described in step (4), the vigor of neutral protease is 150,000 u/g, the weight of described neutral protease and Radix Rhodiolae powder is 1:100; The aqueous hydrochloric acid solution using mass fraction to be 10% in step (4) regulates pH; Temperature retention time described in step (4) is 3-3.5h.
7. the weight ratio of the extracting method of Radix Rhodiolae entity small-molecular peptides according to claim 1, it is characterised in that pepsic vigor described in step (5) is 50,000 u/g, described pepsic weight and Radix Rhodiolae powder is 1:100; In step (5), operating weight mark is the aqueous hydrochloric acid solution adjustment pH of 10%; Temperature retention time described in step (5) is 3-3.5h.
8. the extracting method of Radix Rhodiolae entity small-molecular peptides according to claim 1, it is characterised in that described in step (6), the time of insulation is 15-20min; Described being filtered into is filtered with the defecator of 300 order-500 orders.
9. the extracting method of Radix Rhodiolae entity small-molecular peptides according to claim 1, it is characterized in that, adding the activated carbon accounting for described filtrate weight 2%-3% in step (7) in filtrate, at the temperature of 65-75 DEG C, stirring decolouring 25-35min, then filters to obtain supernatant liquid; Concentrating clarifying liquid is to its former ponderable 15%-25%, then is dried to obtain Radix Rhodiolae entity small-molecular peptides powder.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112999316A (en) * | 2019-12-20 | 2021-06-22 | 近晟（上海）医药科技有限公司 | Activated cell cold compress patch prepared by CF activated cell extraction method |
| CN114796089A (en) * | 2022-05-26 | 2022-07-29 | 格莱康美生物医学技术(北京)有限公司 | Application of umbilical cord mesenchymal stem cell exosome and rhodiola rosea polypeptide in beauty treatment and face nourishing |
| CN114796089B (en) * | 2022-05-26 | 2024-05-14 | 格莱康美生物医学技术(北京)有限公司 | Application of umbilical cord mesenchymal stem cell exosome combined rhodiola rosea polypeptide in maintaining beauty and keeping young |
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| CN102000121A (en) * | 2010-11-05 | 2011-04-06 | 北京中生奥普寡肽技术研究所 | Cordyceps militaris oligopeptide composition as well as preparation method and use thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102000121A (en) * | 2010-11-05 | 2011-04-06 | 北京中生奥普寡肽技术研究所 | Cordyceps militaris oligopeptide composition as well as preparation method and use thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112999316A (en) * | 2019-12-20 | 2021-06-22 | 近晟（上海）医药科技有限公司 | Activated cell cold compress patch prepared by CF activated cell extraction method |
| CN114796089A (en) * | 2022-05-26 | 2022-07-29 | 格莱康美生物医学技术(北京)有限公司 | Application of umbilical cord mesenchymal stem cell exosome and rhodiola rosea polypeptide in beauty treatment and face nourishing |
| CN114796089B (en) * | 2022-05-26 | 2024-05-14 | 格莱康美生物医学技术(北京)有限公司 | Application of umbilical cord mesenchymal stem cell exosome combined rhodiola rosea polypeptide in maintaining beauty and keeping young |
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