CN1056412C

CN1056412C - Chemically modified chitin activating carriers

Info

Publication number: CN1056412C
Application number: CN96120646A
Authority: CN
Inventors: 宋杨; 侯司; 汪姗霖; 赵辉; 陶沪蓉; 袁克文
Original assignee: BIOLOGICAL INSTITUTE ANHUI PROVINCE
Current assignee: BIOLOGICAL INSTITUTE ANHUI PROVINCE
Priority date: 1996-11-15
Filing date: 1996-11-15
Publication date: 2000-09-13
Anticipated expiration: 2016-11-15
Also published as: CN1155581A

Abstract

The present invention relates to a chemically modified chitosan active carrier. Chitosan, each monomolecular glucose structure of which only has one acetamido or amino, is chemically modified by formaldehyde, epichlorohydrin and ammonia, and then is activated by glutaraldehyde to form a chemically modified chitosan active carrier with four active aminos on each monomolecular glucose structure. Particularly, the chemically modified chitosan active carrier has the highest activity and is very suitable for preparing trasylol with high purity.

Description

The chitin of chemical modification and modification and chitosan

The present invention relates to the chemical modification chitin activating carriers, particularly the chitosan of chemical modification and modification or chitin and chitosan.

As everyone knows, the molecular formula of chitosan or chitin such as figure below.

There is active-NH on the glucose unit structure in their molecules ₂The base or-NH-CO-CH ₃Base, and do not have two adjacent-OH bases, if without chemical modification, and adopt the activation method of existing various carriers, as C.A.Vol.109,1988,88858m and C.A.Vol.96,1982, the ordinary method that 195855V mentioned, the former only adopts and defends dialdehyde and carry out modification, and the latter adopts the calcium salt method of removing, be be difficult to prepare have large vol, high reactivity, carrier that the non-specific adsorption ability is low, thereby limited its effect and purposes.

The object of the present invention is to provide the chitin and the chitosan of a kind of chemical modification and modification.The chitosan kind carrier of resulting chemical modification and modification has active height, capacity is big, so can become the novel active carrier of affinity adsorbent and immobilized enzyme effectively.

Embodiment of the present invention are as follows: (chitin is a kind of natural polymer, generally contain the chitosan about 20%, and general chitosan generally contains 20% chitin, below example be example with the chitosan.)

(1) chemical modification: chitosan.On its modular construction glucose molecule of solution usefulness copper sulfate complex protection-NH ₂Base, crosslinked with defending dialdehyde then, make it to become the chitosan kind complex compound that has insoluble amino;

(2) chemically modified: go complexing with hydrochloric acid, react with excess formaldehyde then, make C on the glucose unit structure ₂On-NH ₂Change into-NH (CH ₂(OH)) or-N (CH ₂(OH)) ₂, its reaction formula is as follows:

R represents and removes hydroxyl and amino residue (as follows) on the glucosyl group in the formula.

With sodium borohydride remove superfluous formaldehyde again with Epicholorohydrin and ammoniacal liquor elder generation afterreaction, make-the OH base changes into-NH ₂, its reaction formula is as follows:

The glucose unit structure has only one-NH from above-mentioned original as can be seen chitosan molecule ₂Become four and have the active amine groups that contains.For chitin because C ₂On be not amino but acetamido, so need not with the copper sulfate complex protection and need not to add hydrochloric acid to go complexing and add oxidation of formaldehyde, directly and sodium borohydride react and form-NH (CH ₂(OH)) structurally slightly different with Epicholorohydrin and the reacted product of ammoniacal liquor, promptly be

Only contain three amino as can be seen.

(3) activation: will the above-mentioned modification that contains three or four amino, the chitosan (or chitin) after modifying and defend two aldehyde reactions, the residual dialdehyde of defending of flush away promptly can be used as a plurality of-NH of coupling protein matter or polypeptide (enzyme, antibody hormone, enzyme inhibitors etc.) molecule ₂The novel active carrier.

With preference the present invention is described in detail below, but does not mean that limitation of the scope of the invention:

Example 1: the acetic acid that takes by weighing 1000 milliliter 1% of 10 gram chitosans adding in one 2000 milliliters beaker stirs and makes its dissolving, filtered liquid adds 10% copper sulfate, 1500 ml solns, stir and left standstill 20 hours in 30 minutes, supernatant liquor inclines, the throw out distilled water wash, add 10% then and defend 10 milliliters of dialdehyde solution, restir 2 hours, leach solid substance, use distilled water wash, vacuum-drying in general moisture eliminator, pulverized 100 mesh sieve branches, obtain granulous modified chitosan complex compound 12 grams, get wherein and 10 restrain 6% hydrochloric acid of putting into 40 milliliters of another beaker addings, stir and went complexing in 2 hours, add again 20 milliliter 36% formaldehyde solution at room temperature stirring reaction placed 20 hours down in 5 ℃ after 2 hours, add 2 milligrams of sodium borohydrides, stirring at normal temperature reaction 2 hours adds 10 milliliters of 2N sodium hydroxide again, stirs 2 hours, be washed till neutrality with deionized water, boiled 1 hour, and leached solid substance, put into another beaker, add 6 milliliters of Epicholorohydrins, be heated to 60 ℃ of reactions 3 hours, use deionized water wash then, 20 milliliters of adding distil waters, add 4 milliliters of strong aquas, be incubated 4 hours down at 60 ℃, filter, the chitosan solid substance that gets chemical modification and modification 13.5 restrains totally, get wherein 10 grams put into beaker add 50 milliliter 10% defend dialdehyde and 20 milliliters of borate buffer solutions (0.025 mole, P ^H=7.8), mixing, vacuum lower pumping at 93332 crust, stirring reaction 4 hours is used a large amount of deionized water wash then, again with above-mentioned borate buffer solution washing, be immersed in behind the suction filtration in the same above-mentioned damping fluid, get the chitin carrier of 25 milliliters of high reactivity large vol chemistry modifications and modification, record on each unit molecule glucose structure and on average contain 4 active aminos, the refrigerator that is stored in 0～10 ℃ is interior standby.

Example 2: except that replacing 10 to restrain the chitosans with chitin 12 grams, all the other conditions are identical with example 1, also obtain the chitin carrier of 25 milliliters of chemical modifications and modification, record on each unit molecule glucose structure on average to contain 3.8 active aminos.

The activated carrier that forms of the chitosan kind of chemical modification of the present invention and modification, its advantage are active Amino number was by only having an amino to be increased to four amino on original each unimolecule glucose structure, thereby Improved widely its activity and capacity, be suitable for as the affine adsorbent of preparation, immobilised enzymes novel Active carrier. Such as: can be used as the affine suction that the preparation high-purity presses down peptase, high-purity alpha-interferon etc. Attached dose active carrier.

Claims

1. the chitin of chemical modification and modification is characterized in that following structure:

R represents the residue that removes hydroxyl and kharophen on the glucosyl group in the formula.

2. the chitosan of chemical modification and modification is characterized in that following structure:

R represents to remove hydroxyl and amino residue on the glucosyl group in the formula.