CN105628835A - Method for testing contents of multiple components of medicinal material inula cappa - Google Patents
Method for testing contents of multiple components of medicinal material inula cappa Download PDFInfo
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- CN105628835A CN105628835A CN201510576659.6A CN201510576659A CN105628835A CN 105628835 A CN105628835 A CN 105628835A CN 201510576659 A CN201510576659 A CN 201510576659A CN 105628835 A CN105628835 A CN 105628835A
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Abstract
The invention discloses a method for testing contents of multiple components of a medicinal material inula cappa. The method comprises the following step: testing contents of 6 components, namely, scopoli, 1,3-O-dicaffeoyl-quinic acid, galuteolin, 3,4-O-dicaffeoyl-quinic acid, 3,5-O-dicaffeoyl-quinic acid and 4,5-O-dicaffeoyl-quinic acid in the medicinal material inula cappa by using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), wherein puerarin is taken as internal standard in ultra-high performance liquid chromatography-tandem mass spectrometry analysis. The method for testing the contents of multiple components of the medicinal material inula cappa is established for a first time, the contents of the 6 components in inula cappa can be accurately tested at the same time within 6 minutes through UPLC-MS/MS, and a rapid, sensitive, stable and reliable novel method is provided for quality control on inula cappa.
Description
Technical field
The present invention relates to pharmaceutical compositions to differentiate and technical field of quality detection, be specifically related to a kind of measure the method for Multiple components content in Herba Inulae cappae medical material.
Background technology
Herba Inulae cappae is feverfew Herba Inulae cappae Inulacappa(Buch.-Ham.exD.Don) the dry herb of DC., there is wind-dispelling heat-dissipating, removing toxic substances and promoting subsidence of swelling effect, it is Miao in Guizhou medicinal herbs most in use, Miao medicine is called " Bexniouxdant " (approximate Chinese transliteration is " Herba Inulae cappae "), in " Miao Minority ", " China's book on Chinese herbal medicine Miao Ethnomedicine volume ", 2003 editions " Guizhou Province's Chinese medicines, ethnic drug quality standard " in all record, " the seven logical granules of god's sore throat " that Guizhou Province develops with Herba Inulae cappae for raw material, " BIKANG PIAN ", various Miao Ethnomedicine preparation list marketings in succession such as " the sweet drop pill of sheep ", evident in efficacy, create good economic results in society. in the Dai nationality, Herba Inulae cappae has another name called " surprised ", cures the principal agent of recipe " Yajiao Hadun San " (record in 2010 version Chinese Pharmacopoeia one) for the Dai Nationality. " China National medicine will " is recorded: except Miao ethnic group, the Dai nationality, all there is abundant medicinal experience the minority areas such as Herba Inulae cappae is strong in China, Dong, Jingpo, Lahu, Lisu, Seedling, Yi nationality, Was, it is usually used in cold, fever, laryngopharynx swelling and pain, rheumatalgia, ulcerative carbuncle furunculosis, the disease such as acute mastitis, there is unique curative effect. but the quality control level that Herba Inulae cappae basic research weakness is always up technical problem urgently to be resolved hurrily, particularly Herba Inulae cappae is low, it is difficult to ensure that the quality of Herba Inulae cappae and Related product thereof and curative effect.
Reporting more to the chemical constitution study of Herba Inulae cappae in recent years, its main component has phenols, sesquiterpenoids, organic acid, flavonoid and volatile oil etc., but less about the quality research of Herba Inulae cappae at present. " Guizhou Province's Chinese medicine, ethnic drug quality standard " not yet records the assay project of Herba Inulae cappae, through Literature Consult, also only has Herba Inulae cappae single component and Determination of Total Flavonoids, it is impossible to reflect its inherent quality comprehensively. The prior art indicate that; scopolin in Herba Inulae cappae, 1; 3-O-dicaffeoyl-quinic acid, luteoloside; 3,4-O-dicaffeoyl-quinic acid, 3,5-O-dicaffeoyl-quinic acid and 4; six kinds of compositions such as 5-O-dicaffeoyl-quinic acid; there is the pharmacological actions such as antiinflammatory, antibacterial, antioxidation and antiviral, therefore, set up the content assaying method of above-mentioned 6 compositions in Herba Inulae cappae and the inherent quality for effectively controlling Herba Inulae cappae can provide scientific basis.
Summary of the invention
The invention aims to more comprehensively differentiate and control the quality of Herba Inulae cappae medical material; thering is provided a kind of adopts UPLC-MS/MS method to measure the method for six kinds of compositions in Herba Inulae cappae medical material; with determine in Herba Inulae cappae scopolin in Herba Inulae cappae, 1; 3-O-dicaffeoyl-quinic acid, luteoloside; 3; six kinds of component contents such as 4-O-dicaffeoyl-quinic acid, 3,5-O-dicaffeoyl-quinic acid and 4,5-O-dicaffeoyl-quinic acid.
Based on above-mentioned purpose; the present invention is the multicomponent content assaying method of such a Herba Inulae cappae medical material; the method include adopt Ultra Performance Liquid Chromatography tandem mass spectrum (UPLC-MS/MS) measure simultaneously scopolin in Herba Inulae cappae medical material, 1; 3-O-dicaffeoyl-quinic acid, luteoloside, 3; 4-O-dicaffeoyl-quinic acid, 3; 5-O-dicaffeoyl-quinic acid, 4; the step of the content of 6 compositions of 5-O-dicaffeoyl-quinic acid, includes with puerarin for interior mark at Ultra Performance Liquid Chromatography Tandem Mass Spectrometry Analysis.
Ultra Performance Liquid Chromatography Tandem Mass Spectrometry Analysis adopts WatersBEHC18(2.1mm �� 100mm, 1.7 ��m) chromatographic column.
Ultra Performance Liquid Chromatography Tandem Mass Spectrometry Analysis adopt 0.1% formic acid acetonitrile (A)-0.1% formic acid water (B) carry out gradient elution for mobile phase. The program of this gradient elution is as follows: 0 ~ 1.0min, 5% ~ 15% (A); 1.0 ~ 3.8min, 15% ~ 18% (A); 3.8 ~ 4.0min, 18% ~ 90% (A); 4.0 ~ 5.0min, 90% ~ 5% (A).
Include also including electron spray ionisation source (ESI) at Ultra Performance Liquid Chromatography Tandem Mass Spectrometry Analysis, monitor, with many reactive ions, the step that (MRM) detects. Its Mass Spectrometry Conditions is: adopt electron spray ionisation source (ESI), capillary tube ionization voltage 3kV, ion source temperature 120 DEG C; Remove solvent gas N2, flow velocity 650L/h, removes solvent gas temperature 350 DEG C, ion source temperature 120 DEG C; Collision gas Ar, flow velocity 0.16mL/min; Scan mode is many reactive ions monitoring (MRM).
In the method, the preparation method of inner mark solution is as follows: precision weighs puerarin, by methanol constant volume, it is thus achieved that the storing solution of puerarin; Taking interior mark storing solution in right amount to volumetric flask, by methanol constant volume to scale, be configured to the inner mark solution of 20 �� g/mL, freezen protective is standby.
Need testing solution preparation method is as follows: takes Herba Inulae cappae fine powder and is about 1.0000g, accurately weighed, puts in round-bottomed flask, add 25% ethanol 25mL, weighed weight (is accurate to 0.01g), and heating and refluxing extraction 2h lets cool, weighed weight again, supply the weight of less loss with extracting solution, shake up, filter, the accurate subsequent filtrate 5mL that draws, in evaporating dish, volatilizes solvent; Residue is transferred to 25mL volumetric flask, adds methanol to scale, shakes up, and microporous filter membrane (0.45 ��m) filters, and takes subsequent filtrate, to obtain final product;
Reference substance solution preparation method is as follows:: precision weighs 6 kinds of reference substances such as scopolin respectively, respectively puts in 10mL volumetric flask, adds methanol constant volume to scale place, shakes up; Scopolin (0.504mg/mL), 1; 3-O-dicaffeoyl-quinic acid (1.037mg/mL), luteoloside (0.856mg/mL), 3; 4-O-dicaffeoyl-quinic acid (1.007mg/mL), 3; 5-O-dicaffeoyl-quinic acid (0.502mg/mL), 4,5-O-dicaffeoyl-quinic acid (1.073mg/mL) storing solution.
UPLC-MS/MS specificity is strong, highly sensitive, quick and precisely, it is increasingly being applied in the research of each side such as Chinese crude drug quality control, therefore present invention application UPLC-MS/MS, with puerarin for interior mark, sets up the content assaying method of 6 compositions in Herba Inulae cappae, having no pertinent literature report both at home and abroad, the inherent quality for effectively controlling Herba Inulae cappae provides scientific basis.
In the present invention, UPLC uses the chromatographic column filler of 1.7 ��m of granularities, can significantly improve the efficiency of separation, and UPLC-MS/MS method substantially increases the analysis speed of each composition in sample when not affecting separating effect. The present invention establishes the multi-target ingredient content assaying method of Herba Inulae cappae medical material first, and adopt UPLC-MS/MS method, the content of 6 compositions in Accurate Determining Herba Inulae cappae while of getting final product in 6min, the quality control for Herba Inulae cappae provides a kind of rapid sensitive, reliable and stable new method.
Present invention experiment has been investigated with methanol, dehydrated alcohol, 25% ethanol, 30% ethanol, 50% ethanol, 70% second alcohol and water for Extraction solvent, respectively by surname extraction, reflux, extract, and supersound process, and Herba Inulae cappae is extracted by different time, after measurement result is comprehensively analyzed, finally adopting 25% alcohol heating reflux to extract 2h, the extraction efficiency of 6 kinds of compositions is better.
Accompanying drawing explanation
Fig. 1 is the UPLC-MS/MS collection of illustrative plates of mixing reference substance solution (A) and need testing solution (B); Wherein: 1. scopolin, 2. puerarin, 3.1,3-O-dicaffeoyl-quinic acid, 4. luteoloside, 5.3,4-O-dicaffeoyl-quinic acid, 6.3,4-O-dicaffeoyl-quinic acid, 7.4,5-O-dicaffeoyl-quinic acid.
Detailed description of the invention
In order to clearly the present invention will be described, below by way of being embodied as example, the specific embodiment of the present invention is described in more detail. However, it should be understood that, it is only illustrative for the present invention that the following stated is embodied as example, carrying out any character restriction not for the present invention, wherein material therefor, reagent, instrument and operating condition are only representational, and it is not limited to cited situation. The present invention can be made the change without departing from the claims in the present invention protection defined and improvement by reading following description by person of ordinary skill in the field, and these are changed and improvement is also in present invention scope required for protection.
1 instrument and reagent
1.1 instruments
ACQUITY system Ultra Performance Liquid Chromatography-triple quadrupole rods tandem mass spectrometry instrument, including binary gradient pump, automatic sampler, column oven, triple level Four bar mass analyzer and Masslynx4.1 mass spectrum work station (waters company of the U.S.).
1.2 reagents
Scopolin reference substance (lot number 13061442, purity >=98%) is provided by Shanghai Tongtian Biotechnology Co., Ltd.; 1,3-O-dicaffeoyl-quinic acid, 3,4-O-dicaffeoyl-quinic acid, 3,5-O-dicaffeoyl-quinic acid, 4,5-bis--O-caffeoyl quinic acid reference substance (provides by solid preparation of Chinese medicine manufacturing technology National Engineering Research Centre, lot number respectively 1384-101215,1384-101215, S34-110121,1384-101215, purity >=98%); Puerarin and luteoloside reference substance (providing by National Institute for Food and Drugs Control, lot number is 111-090623,111720-200602 purity >=98% respectively); Methanol (Tianjin Ke Miou chemical reagent company limited), acetonitrile (Merck company of Germany), formic acid (TEDIA company limited of the U.S.) are chromatographically pure; Other reagent are analytical pure, and water is ultra-pure water. Herba Inulae cappae medical material: totally 6 batch sample, derives from the ground such as Guizhou Gao Po, Longli and Luodian, Guiyang Medical College associate professor Long Qingde is accredited as the dry herb of feverfew Herba Inulae cappae Inulacappa (Buch.-Ham.exD.Don) DC..
2 methods and result
2.1 solution preparations
Reference substance solution: precision weighs 6 kinds of reference substances such as scopolin respectively, respectively puts in 10mL volumetric flask, adds methanol constant volume to scale place, shakes up. Scopolin (0.504mg/mL), 1; 3-O-dicaffeoyl-quinic acid (1.037mg/mL), luteoloside (0.856mg/mL), 3; 4-O-dicaffeoyl-quinic acid (1.007mg/mL), 3; 5-O-dicaffeoyl-quinic acid (0.502mg/mL), 4,5-O-dicaffeoyl-quinic acid (1.073mg/mL) storing solution.
Inner mark solution: precision weighs puerarin (1.148mg), by methanol constant volume to 10mL. Obtain the storing solution of puerarin (0.1148mg/mL). Take interior mark storing solution in right amount to 10mL volumetric flask, by methanol constant volume to scale, be configured to the inner mark solution of 20 �� g/mL, put refrigerator (-20 DEG C) and preserve, standby.
Need testing solution: take Herba Inulae cappae fine powder and be about 1.0000g, accurately weighed, put in round-bottomed flask, add 25% ethanol 25mL, weighed weight (is accurate to 0.01g), and heating and refluxing extraction 2h lets cool, weighed weight again, supply the weight of less loss with extracting solution, shake up, filter, the accurate subsequent filtrate 5mL that draws, in evaporating dish, volatilizes solvent; Residue is transferred to 25mL volumetric flask, adds methanol to scale, shakes up, and microporous filter membrane (0.45 ��m) filters, and takes subsequent filtrate, to obtain final product.
2.2 chromatographic conditions
Chromatographic column: WatersBEHC18(2.1mm �� 100mm, 1.7 ��m) post, guard column: WatersVanGuardBEHC18(2.1m �� 5mm, 1.7 ��m); Column temperature: 45 DEG C; Flow velocity: 0.35mL/min; Sampling volume is 1 �� L; Mobile phase: 0.1% formic acid acetonitrile (A)-0.1% formic acid water (B), gradient elution, elution program is as follows: 0 ~ 1.0min, 5% ~ 15% (A); 1.0 ~ 3.8min, 15% ~ 18% (A); 3.8 ~ 4.0min, 18% ~ 90% (A); 4.0 ~ 5.0min, 90% ~ 5% (A).
2.3 Mass Spectrometry Conditions
Adopt electron spray ionisation source (ESI), capillary tube ionization voltage 3kV, ion source temperature 120 DEG C; Remove solvent gas N2, flow velocity 650L/h, removes solvent gas temperature 350 DEG C, ion source temperature 120 DEG C; Collision gas Ar, flow velocity 0.16mL/min. Scan mode is many reactive ions monitoring (MRM), and monitoring ion is in Table 1.
Table 1 Mass Spectrometry Conditions
Table 1 note: analyte 1-7 respectively scopolin, 1,3-O-dicaffeoyl-quinic acid, luteoloside, 3,4-O-dicaffeoyl-quinic acid, 3,5-O-dicaffeoyl-quinic acid, 4,5-O-dicaffeoyl-quinic acid and puerarin.
2.4 specificity tests
Accurate absorption mixing reference substance solution and need testing solution in right amount and add interior mark (puerarin) solution, and 15000rpm, centrifugal 5min takes supernatant 1 �� L sample introduction, and result is shown in Fig. 1. As seen from the figure, separating good, do not have impurity to disturb, compare need testing solution and the parent ion of reference substance, daughter ion mass spectrum figure between determined composition, both are consistent, it was shown that this law specificity is good.
2.5 linear tests
Under accurate absorption " 2.4 " item, the mixing reference substance solution of preparation is appropriate, dilutes successively, prepares the series concentration linear work liquid of 6 concentration, respectively sample introduction mensuration. With reference substance concentration (c) for abscissa X, chromatographic peak area and interior mark peak area ratio (A/Ai) are vertical coordinate Y, carry out linear regression, obtain regression equation in Table 2.
The linear relationship of 26 kinds of compositions of table
| Compound Group | Regression equation Regression equation | r | Range of linearity Liner range (�� g/mL) |
| 1 | Y=1.6460X+0.0839 | 0.9998 | 0.058��42.000 |
| 2 | Y=0.3294X-0.1302 | 0.9998 | 0.178��129.630 |
| 3 | Y=0.9855X-0.1096 | 0.9997 | 0.087��63.400 |
| 4 | Y=0.2931X+0.0542 | 0.9994 | 0.288~69.930 |
| 5 | Y=3.6463X+1.4049 | 0.9993 | 0.172��125.500 |
| 6 | Y=0.5351X-0.4644 | 0.9997 | 0.184��134.130 |
Table 2 note: analyte 1-6 respectively scopolin, 1,3-O-dicaffeoyl-quinic acid, luteoloside, 3,4-O-dicaffeoyl-quinic acid, 3,5-O-dicaffeoyl-quinic acid and 4,5-O-dicaffeoyl-quinic acid
22.6 replica test
Take with batch Herba Inulae cappae medical material, by method 6 parts of need testing solutions of parallel preparation under " 2.1 " item, after process, sample introduction measures respectively, 1 ~ 6 average content respectively 0.211,2.083 is calculated according to internal standard method, 0.036,2.573,0.087 and 0.233mg/g, RSD respectively 5.27%, 4.24%, 3.16%, 1.51%, 1.53% and 2.96%, it was shown that this method repeatability is good.
2.7 precision tests
Take the need testing solution of the same lot number consistent with repeatability, METHOD FOR CONTINUOUS DETERMINATION 3d, every day, sample introduction 6 times, calculated content according to internal standard method, as a result 1 ~ 6 in a few days and day to day precision (n=6) RSD respectively 1.02% ~ 3.31% and 1.35% ~ 3.11%, it was shown that instrument precision is good.
2.8 stability tests
Take same need testing solution, after processing respectively in 0,2,4,8,12h sample introduction analyze, the RSD of 1 ~ 6 content respectively 1.94%, 1.54%, 3.49%, 1.87%, 2.83%, 5.79%, it was shown that need testing solution is good at 12h internal stability.
2.9 average recovery tests
The Herba Inulae cappae sample that precision weighs 6 parts of known content is appropriate, it is equally divided into 3 groups, respectively precision add 0.5,1.0,2.0mL mix reference substance solution (1 ~ 6 concentration respectively 0.042,0.130,0.063,0.210,0.126,0.134mg/mL), make the quality-control sample of basic, normal, high concentration, by legal system available test sample solution below " 2.1 " item, measure by above-mentioned condition sample introduction, calculate average recovery, result table 3.
Table 3 response rate experimental result (%, n=3)
2.10 sample determinations
Prepare the need testing solution of 6 batch samples by method below " 2.1 " item, after process, sample introduction measures respectively, calculates the content of each composition in sample according to internal standard method, and result is in Table 4.
The content (mg/g, n=3) of 6 compositions in table 46 batch sample
3 discuss
UPLC uses the chromatographic column filler of 1.7 ��m of granularities, can significantly improve the efficiency of separation, and UPLC-MS/MS method substantially increases the analysis speed of each composition in sample when not affecting separating effect. This research establishes the multi-target ingredient content assaying method of Herba Inulae cappae medical material first, and adopt UPLC-MS/MS method, the content of 6 compositions in Accurate Determining Herba Inulae cappae while of getting final product in 6min, the quality control for Herba Inulae cappae provides a kind of rapid sensitive, reliable and stable new method.
Experiment is investigated with methanol, dehydrated alcohol, 25% ethanol, 30% ethanol, 50% ethanol, 70% second alcohol and water for Extraction solvent, respectively by surname extraction, reflux, extract, and supersound process, and Herba Inulae cappae is extracted by different time, after measurement result is comprehensively analyzed, finally adopting 25% alcohol heating reflux to extract 2h, the extraction efficiency of 6 kinds of compositions is better.
Be can be seen that by the 6 of Guizhou different sources batches of Herba Inulae cappae medical material assay results, in Herba Inulae cappae there is notable difference in the content of 6 kinds of compositions, the medical material content that wherein is produced from Longli, Guizhou is higher, illustrates that ecological environment, collecting time and condition of storage etc. are likely to content is produced impact. The above results is pointed out, and for ensureing the concordance of quality between Herba Inulae cappae related preparations batch, should strengthen the quality control of Herba Inulae cappae, fix the source of medical material if desired.
Claims (8)
1. the multicomponent content assaying method of a Herba Inulae cappae medical material; it is characterized in that: described method include adopt Ultra Performance Liquid Chromatography tandem mass spectrum (UPLC-MS/MS) measure simultaneously scopolin in Herba Inulae cappae medical material, 1; 3-O-dicaffeoyl-quinic acid, luteoloside, 3; 4-O-dicaffeoyl-quinic acid, 3; 5-O-dicaffeoyl-quinic acid, 4; the step of the content of 6 compositions of 5-O-dicaffeoyl-quinic acid, includes with puerarin for interior mark at Ultra Performance Liquid Chromatography Tandem Mass Spectrometry Analysis.
2. the multicomponent content assaying method of Herba Inulae cappae medical material according to claim 1, it is characterised in that: in Ultra Performance Liquid Chromatography Tandem Mass Spectrometry Analysis, adopt WatersBEHC18(2.1mm �� 100mm, 1.7 ��m) chromatographic column.
3. the multicomponent content assaying method of Herba Inulae cappae medical material according to claim 1, it is characterised in that: adopt 0.1% formic acid acetonitrile (A)-0.1% formic acid water (B) to carry out gradient elution for mobile phase in Ultra Performance Liquid Chromatography Tandem Mass Spectrometry Analysis.
4. the multicomponent content assaying method of Herba Inulae cappae medical material according to claim 3, it is characterised in that: the program of described gradient elution is as follows: 0 ~ 1.0min, 5% ~ 15% (A); 1.0 ~ 3.8min, 15% ~ 18% (A); 3.8 ~ 4.0min, 18% ~ 90% (A); 4.0 ~ 5.0min, 90% ~ 5% (A).
5. the multicomponent content assaying method of Herba Inulae cappae medical material according to claim 1, it is characterized in that: include also including electron spray ionisation source (ESI) at Ultra Performance Liquid Chromatography Tandem Mass Spectrometry Analysis, monitor, with many reactive ions, the step that (MRM) detects.
6. the multicomponent content assaying method of Herba Inulae cappae medical material according to claim 1, it is characterised in that:
The preparation method of described inner mark solution is as follows: precision weighs puerarin, by methanol constant volume, it is thus achieved that the storing solution of puerarin; Taking interior mark storing solution in right amount to volumetric flask, by methanol constant volume to scale, be configured to the inner mark solution of 20 �� g/mL, freezen protective is standby.
7. the multicomponent content assaying method of Herba Inulae cappae medical material according to claim 5, it is characterised in that:
Described electron spray ionisation source (ESI), the Mass Spectrometry Conditions monitoring the step that (MRM) detects with many reactive ions is: adopt electron spray ionisation source (ESI), capillary tube ionization voltage 3kV, ion source temperature 120 DEG C; Remove solvent gas N2, flow velocity 650L/h, removes solvent gas temperature 350 DEG C, ion source temperature 120 DEG C; Collision gas Ar, flow velocity 0.16mL/min; Scan mode is many reactive ions monitoring (MRM).
8. the multicomponent content assaying method of the Herba Inulae cappae medical material according to any one of claim 1-7, it is characterised in that: the method also includes the step of the preparation of need testing solution and reference substance solution;
Prepared by need testing solution: take Herba Inulae cappae fine powder and be about 1.0000g, accurately weighed, puts in round-bottomed flask, add 25% ethanol 25mL, weighed weight (is accurate to 0.01g), and heating and refluxing extraction 2h lets cool, weighed weight again, supply the weight of less loss with extracting solution, shake up, filter, the accurate subsequent filtrate 5mL that draws, in evaporating dish, volatilizes solvent; Residue is transferred to 25mL volumetric flask, adds methanol to scale, shakes up, and microporous filter membrane (0.45 ��m) filters, and takes subsequent filtrate, to obtain final product;
Prepared by reference substance solution: precision weighs 6 kinds of reference substances such as scopolin respectively, respectively puts in 10mL volumetric flask, adds methanol constant volume to scale place, shakes up; Scopolin (0.504mg/mL), 1; 3-O-dicaffeoyl-quinic acid (1.037mg/mL), luteoloside (0.856mg/mL), 3; 4-O-dicaffeoyl-quinic acid (1.007mg/mL), 3; 5-O-dicaffeoyl-quinic acid (0.502mg/mL), 4,5-O-dicaffeoyl-quinic acid (1.073mg/mL) storing solution.
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| CN108508102A (en) * | 2018-02-05 | 2018-09-07 | 贵州医科大学 | The assay method of nine kinds of effect components plasma protein binding rates in Sheepear Inula Herb extract |
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| áRPÁD KöNCZöL 等: "Antioxidant activity-guided phytochemical investigation of Artemisia gmelinii Webb. ex Stechm.: Isolation and spectroscopic challenges of 3,5-O-dicaffeoyl (epi?) quinic acid and its ethyl ester", 《JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS》 * |
| TAO YI 等: "Comparison of the Chemical Composition and Pharmacological Effects of the Aqueous and Ethanolic Extracts from a Tibetan "Snow Lotus" (Saussurea laniceps) Herb", 《MOLECULES》 * |
| 何迅 等: "羊耳菊药材中4,5-O-二咖啡酰基奎宁酸的含量测定", 《中国新药杂志》 * |
| 熊荻菲菲 等: "羊耳菊药材的 HPLC 指纹图谱研究", 《中国中药杂志》 * |
| 郑林 等: "UPLC-MS 法同时测定大鼠血浆中氧化芍药苷、没食子酸及咖啡酰基奎宁酸类成分", 《中成药》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107561196A (en) * | 2017-09-04 | 2018-01-09 | 贵州医科大学 | HPLC methods that are a kind of while determining 7 kinds of component contents in Blumea balsamifera |
| CN108508102A (en) * | 2018-02-05 | 2018-09-07 | 贵州医科大学 | The assay method of nine kinds of effect components plasma protein binding rates in Sheepear Inula Herb extract |
| CN109100432A (en) * | 2018-02-05 | 2018-12-28 | 贵州医科大学 | Nine kinds of effective components are absorbed and the research method of transporting mechanism in Caco-2 cell model in Sheepear Inula Herb extract |
| CN110954622A (en) * | 2019-12-20 | 2020-04-03 | 成都普思检验检测有限公司 | Method for determining content of 1, 3-dicaffeoylquinic acid in inula flower |
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|---|---|
| CN105628835B (en) | 2018-02-09 |
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