CN105628482A - Method and device for diluting concentrated diluent used by blood cell analyzer - Google Patents
Method and device for diluting concentrated diluent used by blood cell analyzer Download PDFInfo
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- CN105628482A CN105628482A CN201610059586.8A CN201610059586A CN105628482A CN 105628482 A CN105628482 A CN 105628482A CN 201610059586 A CN201610059586 A CN 201610059586A CN 105628482 A CN105628482 A CN 105628482A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
Abstract
The invention relates to a method and device for diluting a concentrated diluent used by a blood cell analyzer. The device comprises an upper water tank (8) connected with a lower water tank (11) through a connecting solenoid valve (9), wherein the upper water tank (8) is connected with a pure water maker (2) through a water injection solenoid valve (3) and a concentrated diluent bag (13) through a peristaltic pump (16); an upper water tank liquid level probe (14), a stirring device (22), a detecting system and a constant temperature system are arranged on the upper water tank (8); the detecting system is composed of a conductivity detecting electrode (15) and a PH meter probe (19); the constant temperature system is composed of a heater (23), a temperature protection switch (5) and a temperature sensor (6); and a lower outlet (12) of the lower water tank (11) is connected with a diluent inlet (1) of the blood cell analyzer through a pipe and is provided with a lower water tank liquid level floater sensor (10). The concentrated diluent in small bags can be automatically diluted into common diluent applicable to the analyzer at a user terminal, so that the logistics cost is greatly saved, and the user experience is not influenced through the automatic diluting process.
Description
Technical field
The present invention relates to blood of human body detecting instrument technical field, particularly a kind of blood cell analyzer uses dilution process and the device of concentration and dilution liquid.
Background technology
Blood cell analyzer belongs to medical precision instrument, is used for measuring the relevant information of blood cell. In routine use, diluent is the Principal diluent in its detection process, is also the main wash agent in instrument maintenance process, so the daily consumption of diluent is relatively larger. Current instrument is all directly insert instrument pipette in the common diluent bucket that drum is packed to use when using, and often makes to be finished one barrel and just changes once. The common packaging of common diluent is every barrel of 20L, within general 5-6 days, one barrel will be changed, thus user often to buy common diluent or once make a big purchase in large quantities, calculate according to the weight and volume of common diluent, its logistics transportation is costly, adds the use cost of user undoubtedly. And the concentration and dilution liquid packaging volume of high concentration is little, convertible dilution liquid measure is many, and then its cost of transportation will greatly reduce. If user has the pure water making machine of other instrument adapteds, then select the testing cost using the concentration and dilution liquid of high concentration will be substantially reduced blood cell analyzer.
Summary of the invention
It is an object of the invention to provide a kind of blood cell analyzer reducing blood cell analyzer use cost and use dilution process and the device of concentration and dilution liquid.
It is an object of the invention to be realized by following approach:
Blood cell analyzer uses the air mix facilities of concentration and dilution liquid, it includes being connected lower header 11 by upper water box 8 through connection electromagnetic valve 9, described upper water box 8 connects pure water making machine 2 equipped with through water injecting electromagnetic valve 3, concentration and dilution liquid bag 13 is connected through peristaltic pump 16, and equipped with upper water box level probe 14 and agitating device 22, the detection system that probe 19 forms is counted, equipped with the constant temperature system being made up of heater 23, temperature protection switch 5, temperature sensor 6 equipped with by conductivity sensing electrodes 15 and PH; Lower part outlet 12 at described lower header 11 connects blood cell analyzer diluent import 1 by the road, and equipped with lower header liquid level float sensor 10.
It is further that described peristaltic pump 16 connects on the pipeline of upper water box 8 equipped with detection optocoupler 17.
It is further that connecting tee joint 18 is followed by air vent by the road equipped with upper water box gas outlet 20 and lower header gas outlet 24 on described upper water box 8 and lower header 11.
It is further that described agitating device 22 is made up of driven by motor stirring paddle.
It is further that the volume of described lower header 11 is more than upper water box 8.
Blood cell analyzer uses the dilution process of concentration and dilution liquid, and it includes being connected lower header 11 by upper water box 8 through connection electromagnetic valve 9, and dilution process is as follows:
A. in upper water box 8, dilute concentration and dilution liquid:
1.. when the diluent in lower header 11 uses setting value, in lower header 11, liquid level float sensor 10 will send request signal, open peristaltic pump 16 and quantitatively add concentration and dilution liquid in upper water box 8, open water injecting electromagnetic valve 3 simultaneously and be automatically injected pure water in upper water box 8, level probe 14 cuts out water injecting electromagnetic valve 3 immediately after signal being detected, opening agitating device 22 to mix, when liquid level covers heater 23, constant temperature system is started working;
2.. when mixing completes, when heating temperatures is to design temperature T, the conductivity sensing electrodes 15 of detection system starts to detect the electrical conductivity of diluent, and PH counts probe 19 and detects diluent pH value, parameter meets range of set value and then completes the dilution of concentration and dilution liquid, otherwise reports to the police;
3.. after completing dilution, open the connection electromagnetic valve 9 connecting lower header 11, the diluent in upper water box 8 is discharged into lower header 11 for instrument;
B. when concentration and dilution liquid bag needs after being finished to change concentration and dilution liquid bag:
1.. detect just report to the police after without concentration and dilution liquid when peristaltic pump 16 connects the detection optocoupler 17 on upper water box 8 pipeline, close the request signal of lower header liquid level sensor 10, manually change concentration and dilution liquid bag;
2.. click upper computer software and change diluent packet function, start peristaltic pump 16 and irrigate concentration and dilution liquid to its pipeline, when in blood cell analyzer, diluent detection sensor detects after without diluent, emptying upper water box 8 and lower header 11, open water injecting electromagnetic valve 3, in upper water box 8 inject pure water rinsing, then open connection electromagnetic valve 9 liquid in upper water box 8 is poured lower header 11, take the liquid in upper water box 8 and lower header 11 away emptying with the waste drains pump in blood cell analyzer;
3., after diluting concentration and dilution liquid in upper water box 8, the diluent in upper water box 8 is discharged into lower header 11 for instrument.
Being further described pure water when being automatically injected upper water box 8, pure water injection rate is controlled by feed liquor flow resistance, and when the diluent in upper water box 8 is discharged into lower header 11, rate of release is controlled by flow resistance, it is achieved liquid level steadily rises.
The positive effect of the present invention: the scheme of present invention proposition and blood cell analyzer thereof use dilution process and the device of concentration and dilution liquid, common diluent can be become for instrument at user side automatic dilution the concentration and dilution liquid of fractional pack, which save the common diluent of the long-time high-frequency buying ship bulky of user, it is greatly saved logistics cost, the dilution of automatization also makes Consumer's Experience unaffected, the daily common diluent being supplied to blood cell analyzer use is changed into concentration and dilution liquid and realizes user side automatic dilution, and instrument interior structure need not be changed, not only reduce user cost but also do not affect Consumer's Experience, realize the serial dilution with bag concentration and dilution liquid and use, waste diluent when need not be interrupted instrument test and avoid changing, change concentration and dilution liquid bag dilution process, sufficiently and reasonably consider the occupation mode under concentration and dilution liquid different situations, the accuracy of the diluent parameter after dilution can be detected under isoperibol, one-time detection is carried out again under the premise guaranteeing performance of dispatching from the factory at concentration and dilution liquid, it is further ensured that the performance of diluent.
Accompanying drawing explanation
Fig. 1 is technical scheme schematic diagram
Fig. 2 is the concentration and dilution liquid dilution flow chart of the present invention
Fig. 3 is the replacing concentration and dilution liquid bag dilution flow chart of the present invention
Fig. 4 is assembly of the invention schematic diagram
In figure: 1. the import of blood cell analyzer diluent, 2. pure water making machine, 3. water injecting electromagnetic valve; 4. joint, 5. temperature protection switch, 6. temperature sensor; 7. dilution water tank, 8. upper water box, 9. connection electromagnetic valve; 10. liquid level float sensor, 11. lower headers, 12. lower header bottom fittings; 13. concentration and dilution liquid bag; 14. upper water box level probe, 15. conductivity sensing electrodes, 16. peristaltic pumps; 17. detection optocoupler; 18. three way cock, 19.PH counts probe, 20. upper water box gas outlets; 21. upper water box joint; 22. agitating device, 23. heaters, 24. lower header gas outlets
Detailed description of the invention
Below in conjunction with Figure of description, the present invention is elaborated:
For making the purpose of the present invention, technical scheme and effect clearly, clearly, devise a kind of device so that the present invention program to be described in detail further. Should be appreciated that described device is only the one that this technical scheme realizes, be not intended to limit the present invention.
The overall plan realization means of the present invention is: designs a kind of blood cell analyzer and uses air mix facilities and the dilution process of concentration and dilution liquid, make user side adopt quantitative pure water to add quantitative concentration and dilution liquid fully mix in a vessel and complete parameter detecting, become common diluent, resupply blood cell analyzer to use, as shown in Figure 1.
The blood cell analyzer that the present invention program relates to uses dilution process and the device of concentration and dilution liquid, its device is as shown in Figure 4, including: blood cell analyzer diluent import 1, pure water making machine 2, water injecting electromagnetic valve 3, temperature protection switch 5, temperature sensor 6, dilution water tank 7 wherein comprises upper water box 8 and lower header 11, connection electromagnetic valve 9, lower header liquid level float sensor 10, concentration and dilution liquid bag 13, upper water box level probe 14, conductivity sensing electrodes 15, peristaltic pump 16, detection optocoupler 17, PH counts probe 19, agitating device 22, heater 23, joint and pipeline. blood cell analyzer diluent import 1 is connected with lower header bottom fitting 12 by pipeline, pure water making machine 2 is connected with water injecting electromagnetic valve 3 by pipeline, and then connect with upper water box top contact 4, when water injecting electromagnetic valve 3 is opened, pure water is injected in upper water box 8 by the pressure in pure water making machine 2 by pure water, be embedded with a upper water box level probe 14 at upper water box end face, when pure water inject liquid level reach upper water box level probe 14 detect position time, be automatically switched off water injecting electromagnetic valve 3, it is achieved pure water quantitatively injects, concentration and dilution liquid bag 13 is connected with upper water box top contact 21 by pipeline, and on its connecting pipeline around the home a peristaltic pump 16 and one detection optocoupler 17, detection optocoupler 17 is near water tank joint 21 end, being used for detecting with or without concentration and dilution liquid, peristaltic pump 16 is used for quantitatively adding concentration and dilution liquid, upper water box 8 top is also embedded with an agitating device 22, a driven by motor stirring paddle, in order to mix pure water and concentration and dilution liquid, upper water box top is also embedded with one group of conductivity sensing electrodes 15, detects the electrical conductivity of mixed liquor after mixing fully, one PH counts probe 19, detects the pH value of mixed liquor after mixing fully, upper water box end face is additionally provided with a gas outlet 20 and connects air. it is embedded with a heater 23 in upper water box 8 side, one temperature sensor 6, one temperature protection switch 5, three constitutes thermostatic control system, in heater 23 heating process, heating is just stopped immediately, if just cutting off heating power supply when temperature control reaches the temperature set by temperature protection switch 5 immediately and reporting to the police when temperature sensor 6 detects set temperature T. between upper water box 8 with lower header 11, arrange one connect electromagnetic valve 9 and connected by upper lower header. it is embedded with a liquid level float sensor 10 at lower header 11 top, and is provided with a joint 24 and connects with the open to atmosphere joint 20 of upper water box and three way cock 18. after upper water box diluent mixes and detects well, after lower header liquid level float sensor 10 triggers signal simultaneously, system automatically opens up connection electromagnetic valve 9 and the diluent of upper water box 8 is put into lower header 11. lower header 11 side bottom has individual joint 12 to connect with blood cell analyzer diluent inlet 1, and blood cell analyzer proportioning device directly draws diluent from lower header. lower header 11 volume is greater than upper water box 8, upper water box level probe 14 arrange status requirement be: its report to the police time, the water injection rate of upper water box 8 just with add concentration and dilution liquid into about certainty ratio relation, the status requirement that the liquid level float sensor 10 of lower header 11 is arranged is: the dilution liquid measure enough time diluting once concentration diluent to upper water box 8 of lower header 11 when it is reported to the police, thus realizing the continuous use of same bag concentration and dilution liquid. heater 23 is to be heated to design temperature T to upper water box 8 liquid after adding pure water, and temperature sensor 6 is to monitor the temperature in upper water box 8 in real time, allows heater 23 stop heating when the set temperature is reached.This device can be external in outside blood cell analyzer, it is possible to is inside connected to the internal use of blood cell analyzer.
Blood cell analyzer uses the dilution process of concentration and dilution liquid, and this dilution process carries out on device as above, specifically includes following content:
Dilution flow process in same bag concentration and dilution liquid. As shown in Figure 2
Because dilution water tank will not be made very big, same bag concentration and dilution liquid will dilute and just can be finished several times. Its step is as follows:
A. request upper water box dilution: when after the diluent only remaining set amount of lower header 11, set amount is analyser used diluent amount in the upper water box dilution time once, lower header liquid level float sensor 10 is with regard to trigger request signal, then upper water box starts mixed diluting liquid.
B. upper water box dilution concentration and dilution liquid: after receiving the request of lower header liquid level float sensor 10, peristaltic pump 16 is opened and is quantitatively added concentration and dilution liquid in upper water box 8, optocoupler 17 on its path monitor in real time with or without, terminate operation once find that concentration and dilution liquid is just reported to the police. Water injecting electromagnetic valve 3 is opened simultaneously, pure water is automatically injected upper water box 8, and pure water injection rate is controlled by feed liquor flow resistance, it is achieved liquid level steadily rises, when upper water box level probe 14 cuts out first liang of three-way electromagnetic valve 3 immediately after signal being detected, thus it is fixed to realize joining of dilution ratio. When liquid level covers heater 23 time, heater is started working simultaneously.
C. stirring and evenly mixing: after dilution proportioning completes, opens agitating device 22 and mixes.
D. parameter detecting: when mixing complete and heating temperatures to design temperature T allowable error �� 1 DEG C time, conductivity sensing electrodes 15 starts to detect the electrical conductivity of the diluent after mixing, and PH counts probe 19 and detects the diluent pH value after mixing simultaneously. Only two parameters all meet this dilution of range of set value and are just counted as merit, otherwise report to the police.
E. transferring diluent: after the whole proportioning of the diluent of upper water box has detected and met requirement, open connection electromagnetic valve 9 and the diluent of upper water box is discharged into lower header for instrument, rate of release controls to avoid lower header to bubble by flow resistance.
Change the dilution flow process of concentration and dilution liquid bag. As shown in Figure 3
Because the concentration and dilution liquid of different bags effect duration and batch between variant so dilution flow process when it is changed is also different. Its step is as follows:
A. concentration and dilution liquid bag is changed in request: after the optocoupler 17 on concentration and dilution liquid bag 13 path detects without concentration and dilution liquid, alarm manually changes concentration and dilution liquid bag, the diluent of lower header is continuing with simultaneously, and shields the request of the liquid level sensor 10 of lower header.
B. concentration and dilution perfusion: after manually changing concentration and dilution liquid bag, clicks and changes diluent packet function on software, and peristaltic pump 16 rotates full for the perfusion of concentration and dilution liquid path.
C. upper lower header is rinsed in request: when in blood cell analyzer machine, diluent sensor detects without diluent, just open water injecting electromagnetic valve 3 i.e. water purification machine feed water valve to rinse to water filling in upper water box 8, open connection electromagnetic valve 9 again, the liquid of upper water box 8 is poured lower header 11, then utilizes the waste drains pump in blood cell analyzer to take the liquid of upper lower header away emptying. In pure water rinsing, lower header repeatedly and empties and can clean up.
D. upper water box dilution concentration and dilution liquid: after upper lower header clean-drain, shuts connection electromagnetic valve 9; Peristaltic pump 16 is opened and is quantitatively added concentration and dilution liquid in upper water box 8, the optocoupler 17 on its path monitor in real time with or without, terminate operation once find that concentration and dilution liquid is just reported to the police. Water injecting electromagnetic valve 3 is opened simultaneously, pure water is automatically injected upper water box 8, and pure water injection rate is controlled by feed liquor flow resistance, it is achieved liquid level steadily rises, when upper water box level probe 14 cuts out water injecting electromagnetic valve 3 immediately after signal being detected, thus it is fixed to realize joining of dilution ratio. When liquid level covers heater 23 time, heater is started working simultaneously.
E. stirring and evenly mixing: after dilution proportioning completes, opens agitating device 22 and mixes.
F. parameter detecting: when mixing complete and heating temperatures to design temperature allowable error �� 1 DEG C time, conductivity sensing electrodes 15 starts to detect the electrical conductivity of the diluent after mixing, and PH counts probe 19 and detects the diluent pH value after mixing simultaneously. Only two parameters all meet this dilution of range of set value and are just counted as merit, otherwise report to the police.
G. transfer diluent: after the whole proportioning of the diluent of upper water box 8 has detected, open second liang of three-way electromagnetic valve 9 diluent of upper water box 8 is discharged into lower header 11 for instrument perfusion and, rate of release by flow resistance control avoid lower header to bubble.
Not only the present invention program proposes changed into concentration and dilution liquid and realize user side automatic dilution by the daily common diluent being supplied to blood cell analyzer use, and need not change instrument interior structure, reduces user cost but also do not affect Consumer's Experience. Dilution water tank is divided into upper and lower two parts water tank by assembly of the invention, while lower header supplies diluent to blood cell analyzer, upper water box can carry out concentration and dilution liquid dilution action in advance, thus realizing the serial dilution with bag concentration and dilution liquid and use, waste diluent when need not be interrupted instrument test and avoid changing. Upper water box is provided with heater, temperature sensor and temperature protection switch; conductivity sensing electrodes and PH is also had to count electrode; former three constitutes constant temperature system; it is rear that both can detect the accuracy of the diluent parameter after diluting under isoperibol; carry out again one-time detection under the premise guaranteeing performance of dispatching from the factory at concentration and dilution liquid, be further ensured that the performance of diluent. Upper water box is provided with liquid level detection electrode and ensures dilution dosing total amount, concentration and dilution liquid peristaltic pump quantitative filling simultaneously, so that it is guaranteed that the proportion relation of concentration and dilution liquid and pure water. Upper water box is embedded with an evenly mixing device and may insure that concentration and dilution liquid and pure water fully mix. The concentration and dilution liquid bag path with upper water box can detect in real time the presence or absence of concentration and dilution liquid equipped with detection optocoupler. It is embedded with a liquid level float sensor at lower header end face, when lower header diluent is diluted liquid proportioning less than automatically triggering request upper water box time a certain amount of, thus supplementing the dilution liquid measure of lower header in time, and then uninterrupted instrument test. " in same bag concentration and dilution liquid the dilution flow process " and " changing concentration and dilution liquid bag dilution flow process " that the present invention program proposes, sufficiently and reasonably consider the occupation mode under concentration and dilution liquid different situations, compare with common mode and do not affect instrument test and Consumer's Experience.
Claims (7)
1. the air mix facilities of a blood cell analyzer use concentration and dilution liquid, it includes being connected lower header (11) by upper water box (8) through connection electromagnetic valve (9), it is characterized in that connecting pure water making machine (2) equipped with through water injecting electromagnetic valve (3) on described upper water box (8), concentration and dilution liquid bag (13) is connected through peristaltic pump (16), and equipped with upper water box level probe (14) and agitating device (22), the detection system that probe (19) forms is counted equipped with by conductivity sensing electrodes (15) and PH, equipped with by heater (23), temperature protection switch (5), the constant temperature system that temperature sensor (6) forms, lower part outlet (12) in described lower header (11) connects blood cell analyzer diluent import (1) by the road, and equipped with lower header liquid level float sensor (10).
2. blood cell analyzer uses the air mix facilities of concentration and dilution liquid according to claim 2, it is characterised in that described peristaltic pump (16) connects on the pipeline of upper water box (8) equipped with detection optocoupler (17).
3. blood cell analyzer uses the air mix facilities of concentration and dilution liquid according to claim 2, it is characterized in that connecting tee joint (18) is followed by air vent by the road equipped with upper water box gas outlet (20) and lower header gas outlet (24) on described upper water box (8) and lower header (11).
4. blood cell analyzer uses the air mix facilities of concentration and dilution liquid according to claim 2, it is characterised in that described agitating device (22) is made up of driven by motor stirring paddle.
5. blood cell analyzer uses the air mix facilities of concentration and dilution liquid according to claim 2, it is characterised in that the volume of described lower header (11) is more than upper water box (8).
6. blood cell analyzer uses a dilution process for concentration and dilution liquid, and it includes being connected lower header (11) by upper water box (8) through connection electromagnetic valve (9), it is characterized in that dilution process is as follows:
A. in upper water box (8), dilute concentration and dilution liquid:
1.. when the diluent in lower header (11) uses setting value, the interior liquid level float sensor (10) of lower header (11) will send request signal, open peristaltic pump (16) and quantitatively add concentration and dilution liquid in upper water box (8), open water injecting electromagnetic valve (3) simultaneously and be automatically injected pure water in upper water box (8), level probe (14) cuts out water injecting electromagnetic valve (3) immediately after signal being detected, open agitating device (22) to mix, when liquid level covers heater (23), constant temperature system is started working;
2.. when mixing completes, when heating temperatures is to design temperature T, the conductivity sensing electrodes (15) of detection system starts to detect the electrical conductivity of diluent, PH counts probe (19) detection diluent pH value, parameter meets range of set value and then completes the dilution of concentration and dilution liquid, otherwise reports to the police;
3.. after completing dilution, open the connection electromagnetic valve (9) connecting lower header (11), the diluent in upper water box (8) is discharged into lower header (11) for instrument;
B. when concentration and dilution liquid bag needs after being finished to change concentration and dilution liquid bag:
1.. just report to the police after without concentration and dilution liquid when the detection optocoupler (17) on peristaltic pump (16) connection upper water box (8) pipeline detects, close the request signal of lower header liquid level sensor (10), manually change concentration and dilution liquid bag;
2.. click upper computer software and change diluent packet function, start peristaltic pump (16) perfusion concentration and dilution liquid to its pipeline, when in blood cell analyzer, diluent detection sensor detects after without diluent, emptying upper water box (8) and lower header (11), open water injecting electromagnetic valve (3), pure water rinsing is injected in upper water box (8), open connection electromagnetic valve (9) again and the liquid in upper water box (8) is poured lower header (11), the liquid in upper water box (8) and lower header (11) is taken away emptying with the waste drains pump in blood cell analyzer,
3., after diluting concentration and dilution liquid in upper water box (8), the diluent in upper water box (8) is discharged into lower header (11) for instrument.
7. blood cell analyzer uses the dilution process of concentration and dilution liquid according to claim 6, it is characterized in that when described pure water is automatically injected upper water box (8), pure water injection rate is controlled by feed liquor flow resistance, when diluent in upper water box (8) is discharged into lower header (11), rate of release is controlled by flow resistance, it is achieved liquid level steadily rises.
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