CN105624060A - Thuringiensis bacillus fermentation medium - Google Patents
Thuringiensis bacillus fermentation medium Download PDFInfo
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- CN105624060A CN105624060A CN201511016054.8A CN201511016054A CN105624060A CN 105624060 A CN105624060 A CN 105624060A CN 201511016054 A CN201511016054 A CN 201511016054A CN 105624060 A CN105624060 A CN 105624060A
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- bacillus thuringiensis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a thuringiensis bacillus fermentation medium, prepared from the following materials in percentage by weight: 0.5-1.2 percent of sweet potato powder, 2.3-4 percent of powder of distiller's yeast and the balance of water. The medium is simple in composition, and can promote thallus growth and spore formation, and additional adjustment of the pH is not needed when the medium is prepared, thus improving product quality and reducing production cost.
Description
Technical field
The invention belongs to field of microbial culture technology, it relates to a kind of bacillus thuringiensis industrialization liquid forms the Technology of gemma, is specifically related to a kind of bacillus thuringiensis submerged fermentation culture medium being applied to microbial fertilizer.
Background technology
Bacillus thuringiensis is promoting to have vital role in plant growth, enhancing crop disease-resistant ability etc., and is the microorganism of use safety, is therefore widely used in microbial fertilizer production.
Because gemma has the advantage of strong stress resistance, so the microorganism contained in microbial fertilizer exists mainly with spore form. With solid fermentation method easily obtained bacillus thuringiensis gemma, but labour intensity is big, and spore concentration is not high, is generally 0.1 hundred million-1.0 hundred million CFU/g. And effective bacterial content of regulation pulvis and granulated fertilizer is not less than every gram 0.2 hundred million in People's Republic of China (PRC) agricultural industry criteria NY/T798-2004 (composite microbiological fertilizer), effective bacterial content every milliliter of liquid fertilizer is not less than 0.5 hundred million. Utilize liquid submerged fermentation method to cultivate bacillus thuringiensis, cell concentration height, but not easily form gemma, it is necessary to strictly control culture condition, spore forming rate could be improved. The formation of gemma is subject to the impact of each side such as pH, carbon source material concentration, nitrogen source concentration, dissolved oxygen, therefore how to improve the gordian technique that Number of spores in fermented liquid just becomes bacillus thuringiensis fermentation.
The substratum (taking mass ratio range) of general liquid culture bacillus thuringiensis gemma is as starch 2%, corn steep liquor 0.1%, dipotassium hydrogen phosphate 0.5%, ammonium sulfate 0.1%, calcium carbonate 0.25%, manganous sulfate 0.2-0.5%, pH7.2-7.4. Culture condition: 37 �� 2 DEG C, tank pressure 0.05MPa, air flow 1:1.0-1.2 volume/volume/point, inoculum size 1-10%, fermentation 48-72h, spore forming rate 90%. Mainly substratum preparation is comparatively complicated for the shortcoming of this kind of fermention medium, and raw materials cost is higher.
Therefore prior art haves much room for improvement and develops.
Summary of the invention
The present invention provides a kind of bacillus thuringiensis fermention medium, improves culture medium prescription, reduces production cost.
The present invention is found by research, and the ion added in tradition substratum does not need other interpolation by changing substratum composition.
The main nutrient composition of bacillus thuringiensis fermentative medium formula of the present invention is sweet potato powder, distiller's yeast, does not need to add other inorganic salt or trace element.
A kind of bacillus thuringiensis submerged fermentation culture medium for microbial fertilizer, it is characterised in that: by weight percentage, sweet potato powder 0.8-1.5%, distiller's yeast powder 1.5-4%, surplus is water.
Sweet potato powder fineness is not less than 60 orders, and distiller's yeast powder fineness is not less than 40 orders.
Distiller's yeast is flour processing byproduct, cheap. But sweet potato powder wheat directly grinds powder, it is possible to be red dog flour, substratum of the present invention is not containing soybean cake powder, and foam is less, it is not necessary to uses defoamer, reduces production cost.
The Medium's PH Value of the present invention is about 6.0 (after sterilizings), and bacillus thuringiensis is well-grown within the scope of pH5.5-pH8.5, and therefore this substratum is before sterilization without the need to using acid-alkali accommodation pH, simplifies operation.
Carbon-nitrogen ratio in substratum of the present invention is relatively more reasonable, and the potential of hydrogen change of whole fermenting process is little when to stationary phase, and pH is about 5.3-5.5, is conducive to the formation of gemma.
Adopting the substratum of the present invention, make fermentation time from traditional 48-60 hour, shorten to 40-48 hour, the spore concentration of 1m3 fermentor tank is 20-50 hundred million CFU/ml, it is to increase quality product, reduces production cost.
Embodiment
The present invention being described in more detail below by embodiment, these embodiments are only the descriptions to best mode for carrying out the invention, that protection scope of the present invention is not restricted.
Embodiment 1
Adopting (in weight) substratum of following proportioning: sweet potato powder 8 kilograms, 16 kilograms, distiller's yeast, is settled to 0.8M3 with water. Stir after evenly, 121 DEG C of sterilizings 25 minutes, be cooled to 25-40 DEG C, access bacillus thuringiensis seed liquor, fermentation condition by 5% (weight ratio): 37 �� 2 DEG C, ventilation than 1:1.0 volume/volume/point, rotating speed 200-250 rev/min, fermenting 40 hours, residual sugar (Anthrone-sulfuricacid method mensuration) is 5% before inoculation, bacteria concentration (plate dilution assay method, parallel three times) it is on average 38.5 hundred million CFU/ml, spore forming rate is about 90%.
Embodiment 2
Adopting (in weight) substratum of following proportioning: sweet potato powder 12 kilograms, 14 kilograms, distiller's yeast, is settled to 0.8M3 with water. Stir after evenly, 121 DEG C of sterilizings 25 minutes, be cooled to 25-40 DEG C, access bacillus thuringiensis seed liquor, fermentation condition by 5% (weight ratio): 37 �� 2 DEG C, ventilation than 1:1.0 volume/volume/point, rotating speed 280-320 rev/min, fermenting 48 hours, residual sugar (Anthrone-sulfuricacid method mensuration) is 8% before inoculation, bacteria concentration (plate dilution assay method, parallel three times) it is on average 42.4 hundred million CFU/ml, spore forming rate is about 95%.
Embodiment 3
Adopting (in weight) substratum of following proportioning: sweet potato powder 7.2 kilograms, 17 kilograms, distiller's yeast, is settled to 0.8M3 with water. Stir after evenly, 121 DEG C of sterilizings 25 minutes, be cooled to 25-40 DEG C, access bacillus thuringiensis seed liquor, fermentation condition by 5% (weight ratio): 37 �� 2 DEG C, ventilation than 1:1.0 volume/volume/point, rotating speed 300-350 rev/min, fermenting 38 hours, residual sugar (Anthrone-sulfuricacid method mensuration) is 3% before inoculation, bacteria concentration (plate dilution assay method, parallel three times) it is on average 45.6 hundred million CFU/ml, spore forming rate is about 90%. In the description of this specification sheets, at least one embodiment that the description of term " embodiment " etc. means to be contained in the present invention in conjunction with concrete feature, structure, material or feature that this embodiment or example describe or example. In this manual, the schematic representation of above-mentioned term is not necessarily referred to identical embodiment or example. And, the concrete feature of description, structure, material or feature can combine in an appropriate manner in any one or more embodiment or example.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations. Within the spirit and principles in the present invention all, any amendment of doing, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (3)
1. a bacillus thuringiensis fermention medium, it is characterised in that: by weight percentage, sweet potato powder 0.5-1.2%, distiller's yeast powder 2.3-4%, surplus is water.
2. substratum according to claim 1, it is characterised in that: sweet potato powder fineness is not less than 60 orders, and distiller's yeast powder fineness is not less than 40 orders.
3. substratum according to claim 1, it is characterised in that: Medium's PH Value described after sterilizing is about 6.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511016054.8A CN105624060A (en) | 2015-12-30 | 2015-12-30 | Thuringiensis bacillus fermentation medium |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511016054.8A CN105624060A (en) | 2015-12-30 | 2015-12-30 | Thuringiensis bacillus fermentation medium |
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| CN105624060A true CN105624060A (en) | 2016-06-01 |
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| CN201511016054.8A Pending CN105624060A (en) | 2015-12-30 | 2015-12-30 | Thuringiensis bacillus fermentation medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112501083A (en) * | 2020-12-22 | 2021-03-16 | 武汉科诺生物科技股份有限公司 | Bacillus thuringiensis liquid fermentation medium and fermentation culture method |
-
2015
- 2015-12-30 CN CN201511016054.8A patent/CN105624060A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112501083A (en) * | 2020-12-22 | 2021-03-16 | 武汉科诺生物科技股份有限公司 | Bacillus thuringiensis liquid fermentation medium and fermentation culture method |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160601 |
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| WD01 | Invention patent application deemed withdrawn after publication |