CN105614638A - DHA nanoemulsion, preparation method thereof and application of DHA nanoemulsion in healthy health-care beverages - Google Patents
DHA nanoemulsion, preparation method thereof and application of DHA nanoemulsion in healthy health-care beverages Download PDFInfo
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- CN105614638A CN105614638A CN201410711815.0A CN201410711815A CN105614638A CN 105614638 A CN105614638 A CN 105614638A CN 201410711815 A CN201410711815 A CN 201410711815A CN 105614638 A CN105614638 A CN 105614638A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/20—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
- A61K31/202—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids having three or more double bonds, e.g. linolenic
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Abstract
The invention relates to DHA nanoemulsion, a preparation method thereof and application of the DHA nanoemulsion in healthy health-care beverages, and discloses the DHA nanoemulsion. The DHA nanoemulsion is prepared from the following components in parts by weight: 0.5-2 parts of DHA, 1-4 parts of VE, 4-8 parts of a surfactant I, 10-20 parts of a surfactant II, 1-4 parts of VC, 1.5-6 parts of EDTA and 56-82 parts of water, wherein the surfactant I is a surfactant of which the HLB(hydrophilic-lipophilic balance ) range is 1.8-4.7; the surfactant II is a surfactant of which the HLB range is 14.9-16.7; the particle diameter of the DHA nanoemulsion is within 100nm. The invention further discloses the preparation method of the DHA nanoemulsion and the application of the DHA nanoemulsion in the healthy health-care beverages. The DHA nanoemulsion can be directly mixed with water and fruit juice to make a transparent beverage, can also be mixed with emulsions so as to make an emulsion with varied new functions, is stable in nature and is not liable to be oxidated by air.
Description
Technical field
The present invention relates to food technology field, particularly to a kind of DHA nano-emulsion and preparation method thereof and its application in health care beverage.
Background technology
DHA and docosahexenoic acid, belong to Omega-3 type polyunsaturated fatty acid, is that human body can not self synthesize, only by the necessary fatty acid of food intake. The DHA growth important role to the brain of baby, vision and nerve, the fat that can reduce body, the sickness rate reducing ischemic heart desease, the enhancing anti-immunologic function of human body, antiinflammatory, maintain brain function and delay brain aging, and suppress the physiological function that the multiple polyunsaturated fatty acid such as anaphylaxis possesses, increasingly it is subject to people's attention in recent years, and achieves a large amount of achievement under study for action. Current domestic DHA main application or simple emulsion applications in milk powder in solid form, abroad have begun to fashionable with the DHA transparent beverage being composition, but the domestic method that there is no is made the basic reason of transparent beverage and is in that uncontrollable emulsion particle diameter size, naked eyes to the requirement of transparent beverage grain diameter of components generally within 100nm.
DHA contains five active methylene, and very easily the impact of light, oxygen, heat, metallic element and free radical, aoxidizes. Oxidized DHA not only loses original nutrition and pharmaceutical value, and harmful. Wherein peroxide can destroy the DNA in human body and cause canceration, and oxidation product malonaldehyde can make body protein interlinkage make muscle follow the string, and melanin increases, and is the key factor of human senescence; Malonaldehyde and aminated compounds very easily generate the imines class chemical substance with very strong fishy smell, and the smell is awful; The oxide of DHA can also make blood vessel atherosclerotic, damage blood vessel so as to become fragile, and this will be the major reason directly resulting in hypertension and cerebral hemorrhage. Therefore, it is extremely important for making DHA stablize, keep original physiologically active in storage, application process, oils DHA makes the nano-emulsion that stability is high, also has great importance to conveniently storing, widen range of application.
Summary of the invention
It is an object of the invention to provide a kind of with DHA for active component, and stable in properties, the nano-emulsion product used can be added to drinkable water solution/emulsion.
For achieving the above object, the invention provides a kind of DHA nano-emulsion, described DHA nano-emulsion is made up of following parts by weight of component:
DHA:0.5��2 part, VE:1��4 part, surfactant I:4��8 part, surfactant II:10��20 part, VC:1��4 part, EDTA:1.5��6 part, water: 56��82 parts;
Described surfactant I be HLB value scope 1.8��4.7 surfactant; Described surfactant II be HLB value scope 14.9��16.7 surfactant; The particle diameter of described DHA nano-emulsion is within 100nm.
Preferably, the application vector material of described DHA nano-emulsion is drinkable water solution/emulsion, can dissolve each other formation transparency liquid with any ratio aqueous solution.
Preferably, described DHA nano-emulsion is made up of following parts by weight of component:
DHA:0.5g, VE:1g, sorbitan trioleate: 4g, polyoxyethylene 20 sorbitan monooleate: 10g, VC:1g, EDTA:1.5g, water: 82g.
Preferably, described DHA nano-emulsion is made up of following parts by weight of component:
DHA:1.5g, VE:1.5g, sorbitan monooleate: 6g, polyoxyethylene 20 sorbitan monooleate: 14g, VC:3g, EDTA:4g, water: 70g.
Additionally, for achieving the above object, the preparation method that the present invention also provides for a kind of DHA nano-emulsion, described preparation method comprises the steps of
A, DHA, VE and surfactant I are at room temperature stirred mixing by preset proportioning;
B, mixed liquor and surfactant II, VC, EDTA of being formed and water are at room temperature stirred by preset proportioning mix in step A;
C, the mixed liquor homogenizer homogenizing that will be formed in step B, obtain homogenizing fluid, and homogenization pressure is 200-250bar, and flow velocity is 10dm3/h;
D, being sent in microjet instrument by the homogenizing fluid after homogenizing, control pressure is 1350-1400bar, and flow velocity is 40ml/min, and preparing particle diameter is the DHA nano-emulsion within 100nm;
E, adopt aperture be the microporous filter membrane of 0.22 ��m by the above-mentioned DHA nano-emulsion filtration sterilization prepared, obtain purpose DHA nano-emulsion.
Additionally, for achieving the above object, the present invention also provides for a kind of DHA nano-emulsion and is preparing with the application in the transparent and dairy beverage that DHA is active component.
DHA nano-emulsion provided by the invention is a kind of oil-in-water type nano-emulsion, the DHA stable in properties in this nano-emulsion, not easily oxidation by air. Being experimentally verified that, adopt aqueous solution or emulsion that this DHA nano-emulsion makes, its stability relatively DHA oils itself is greatly improved. DHA nano-emulsion provided by the invention can dissolve each other formation transparency liquid with the water of arbitrary proportion, it is possible to adds in the beverages such as daily any a existing water, fruit juice, milk, is greatly broadened the range of application of DHA. Except DHA, possibly together with nutritional benefit compositions such as vitamin E (VE), vitamin Cs (VC), it is achieved the coexistent purpose of multiple nutritional components.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail, and following example are explanation of the invention, the invention is not limited in following example.
DHA nano-emulsion provided by the present invention, adopts following proportioning:
DHA:0.5��2 part, VE:1��4 part, surfactant I:4��8 part, surfactant II:10��20 part, VC:1��4 part, EDTA:1.5��6 part, water: 56��82 parts; Wherein, surfactant I be HLB value scope 1.8��4.7 surfactant; Surfactant II be HLB value scope 14.9��16.7 surfactant; The particle diameter of DHA nano-emulsion is within 100nm.
Surfactant I can select HLB value scope 1.8��4.7 surfactant, the one in the surfactants such as sorbitan trioleate (Span85), dehydration sorbic alcohol tristearate (Span65), propylene glycol monostearate, glyceryl monostearate, sorbitan monooleate (Span80) or sorbitan monostearate (Span60); Surfactant II can select HLB value scope in the surfactant of (14.9��20.0), the one in the surfactants such as polyoxyethylene (20EO) sorbitan monooleate (Tween80), polyoxyethylene (20EO) sorbitan monopalmitate (Tween40), polyoxyethylene (20EO) sorbitan mono-laurate (Tween20) or polyoxyethylene monostearate.
Embodiment 1
The component of DHA nano-emulsion is as follows:
DHA:0.5g, VE:1g, sorbitan trioleate (Span85): 4g, polyoxyethylene (20EO) sorbitan monooleate (Tween80): 10g, VC:1g, EDTA:1.5g, water: 82g.
Embodiment 2
The component of DHA nano-emulsion is as follows:
DHA:1.5g, VE:1.5g, sorbitan monooleate (Span80): 6g, polyoxyethylene (20EO) sorbitan monooleate (Tween80): 14g, VC:3g, EDTA:4g, water: 70g.
Embodiment 3
The component of DHA nano-emulsion is as follows:
DHA:1g, VE:1.5g, propylene glycol monostearate: 4g, polyoxyethylene (20EO) sorbitan monooleate (Tween80) 12g, VC:4g, EDTA:2.5g, water: 75g.
Embodiment 4
The component of DHA nano-emulsion is as follows:
DHA:2g, VE:2g, glyceryl monostearate: 6g, polyoxyethylene (20EO) sorbitan monooleate (Tween80): 15g, VC:2g, EDTA:3g, water: 70g.
Embodiment 5
The component of DHA nano-emulsion is as follows:
DHA:0.5g, VE:3g, sorbitan monooleate (Span80): 6g, polyoxyethylene (20EO) sorbitan monooleate (Tween80): 10g, VC:1.5g, EDTA:2g, water: 77g.
Embodiment 6
The component of DHA nano-emulsion is as follows:
DHA:1g, VE:2.5g, sorbitan monostearate (Span60): 5g, polyoxyethylene (20EO) sorbitan monooleate (Tween80): 13g, VC:2g, EDTA:2.5g, water: 74g.
Embodiment 7
The component of DHA nano-emulsion is as follows:
DHA:2g, VE:1.5g, sorbitan monostearate (Span60): 5.5g, polyoxyethylene (20EO) sorbitan monopalmitate (Tween40): 11g, VC:1g, EDTA:3g, water: 76g.
Embodiment 8
The component of DHA nano-emulsion is as follows:
DHA:1.5g, VE:1g, sorbitan monostearate (Span60): 7g, polyoxyethylene (20EO) sorbitan mono-laurate (Tween20): 10g, VC:2.5g, EDTA:4g, water: 75g.
Embodiment 9
The component of DHA nano-emulsion is as follows:
DHA:0.5g, VE:3g, sorbitan monostearate (Span60): 6g, polyoxyethylene monostearate: 13g, VC:2g, EDTA:1.5g, water: 74g.
DHA nano-emulsion in above example 1-9 all adopts following method to prepare:
A, DHA, VE and surfactant I are at room temperature stirred mixing by preset proportioning;
B, mixed liquor and surfactant II, VC, EDTA of being formed and water are at room temperature stirred by preset proportioning mix in step A;
C, the mixed liquor homogenizer homogenizing that will be formed in step B, obtain homogenizing fluid, and the model of the homogenizer used is ATSEngineeringIncNANOHOMOGENIZEMACHINE, and homogenization pressure is 200-250bar, and flow velocity is 10dm3/h;
D, the homogenizing fluid after homogenizing is sent in microjet instrument, the microjet instrument model used is the instrument of NanoDeBEE45, the control pressure of described microjet instrument is 1350-1400bar, and flow velocity is 40ml/min, and preparing particle diameter is the DHA nano-emulsion within 100nm;
E, adopt aperture be the microporous filter membrane of 0.22 ��m by the above-mentioned DHA nano-emulsion filtration sterilization prepared, obtain purpose DHA nano-emulsion.
Above-mentioned steps E adopts microporous filter membrane by as follows for the operation in detail of DHA nano-emulsion filtration sterilization:
(1) assembling, sterilizing;
The filter membrane in 0.22 ��m of aperture is loaded in the plastics filter cleaned up, screws pressing, stand-by after packaging sterilizing (0.lMPa, 121.5 DEG C of sterilizing 20min).
(2) connect;
By the entrance of the plastics filter after sterilizing aseptically, it is connected on the syringe equipped with solution to be filtered in sterile working's mode, syringe needle is connected with exit and inserts with in the sterile test tube of rubber closure.
(3) filter pressing;
Pressurizeing slowly to clamp-on by the solution to be filtered in syringe is filled in sterile test tube, and filter is finished, and is transferred to by syringe needle. During filter pressing, firmly want suitably, can not be too violent too fast, in order to avoid antibacterial is squeezed through shallow lake glue.
(4) sterility test;
Sterile working draws degerming filtrate 0.1ml on meat soup peptone flat board, and coating uniformly, puts cultivation 24h, check whether there is bacteria growing in 37 DEG C of greenhouses.
(5) clean;
Discard the microporous filter membrane on plastics filter, plastics filter is cleaned up, and change a new microporous filter membrane, assemble wrapping, use after aseptic.
Whole process should aseptically strict aseptic technique, with anti-pollution, during filtration, should avoid each junction that Seepage occurs.
The DHA nano-emulsion adopting above-mentioned preparation method to prepare is carried out stability test, and the condition of stability test is: temperature 25 degree, humidity 70%, climatic chamber. DHA content analyzes method: take measuring samples 1ml, is added thereto to demulsifier dodecyl sodium sulfate 1g, and heating, to 50 DEG C, extracts three times with 10ml petroleum ether after being cooled to room temperature, combining extraction liquid repeatedly. Utilize gas phase analysis content, adopt external standard method. Measurement result is in Table 1, and wherein the data in DHA nano-emulsion one hurdle are the meansigma methods prepared in above-described embodiment 1-9.
Table 1.DHA nano-emulsion stability test synopsis
Result shows: compared to DHA oil solution and DHA neat liquid, DHA nano-emulsion provided by the invention, the content of 88% is still had, hence it is evident that the oil solution (soybean oil or Oleum Arachidis hypogaeae semen) 54% higher than DHA, DHA neat liquid 9%, excellent stability after placing six months.
Application Example 1
The DHA nano-emulsion prepared in above-described embodiment 1-9 is added in the production technology of the beverages such as pure water, fruit juice, milk, carbothiolic acid breast, to detect the interpolation DHA nano-emulsion impact on new product form, the content of the nano-emulsion of the DHA added at the trial is 0.5 ��. Testing result is in Table 2.
Table 2DHA nano-emulsion adds the impact on pure water, fruit juice, milk and carbothiolic acid breast
Test result indicate that the use of DHA nano-emulsion of the present invention will not change the taste of pure water, fruit juice, milk and carbothiolic acid breast; And after six months, content is without significant change.
Except being applied in the beverages such as pure water, fruit juice, milk and carbothiolic acid breast by above-mentioned DHA nano-emulsion, the DHA nano-emulsion of the present invention also can be applicable to the other fields such as food, cosmetics and medicine.
Claims (6)
1. a DHA nano-emulsion, it is characterised in that described DHA nano-emulsion is made up of following parts by weight of component:
DHA:0.5��2 part, VE:1��4 part, surfactant I:4��8 part, surfactant II:10��20 part, VC:1��4 part, EDTA:1.5��6 part, water: 56��82 parts;
Described surfactant I be HLB value scope 1.8��4.7 surfactant; Described surfactant II be HLB value scope 14.9��16.7 surfactant; The particle diameter of described DHA nano-emulsion is within 100nm.
2. DHA nano-emulsion according to claim 1, it is characterised in that the application vector material of described DHA nano-emulsion is drinkable water solution/emulsion, can dissolve each other formation transparency liquid with any ratio aqueous solution.
3. DHA nano-emulsion according to claim 1, it is characterised in that described DHA nano-emulsion is made up of following parts by weight of component:
DHA:0.5g, VE:1g, sorbitan trioleate: 4g, polyoxyethylene 20 sorbitan monooleate: 10g, VC:1g, EDTA:1.5g, water: 82g.
4. DHA nano-emulsion according to claim 1, it is characterised in that described DHA nano-emulsion is made up of following parts by weight of component:
DHA:1.5g, VE:1.5g, sorbitan monooleate: 6g, polyoxyethylene 20 sorbitan monooleate: 14g, VC:3g, EDTA:4g, water: 70g.
5. the preparation method of the DHA nano-emulsion according to any one of a Claims 1-4, it is characterised in that described preparation method comprises the steps of
A, DHA, VE and surfactant I are at room temperature stirred mixing by preset proportioning;
B, mixed liquor and surfactant II, VC, EDTA of being formed and water are at room temperature stirred by preset proportioning mix in step A;
C, the mixed liquor homogenizer homogenizing that will be formed in step B, obtain homogenizing fluid, and homogenization pressure is 200-250bar, and flow velocity is 10dm3/h;
D, being sent in microjet instrument by the homogenizing fluid after homogenizing, the control pressure of described microjet instrument is 1350-1400bar, and flow velocity is 40ml/min, and preparing particle diameter is the DHA nano-emulsion within 100nm;
E, adopt aperture be the microporous filter membrane of 0.22 ��m by the above-mentioned DHA nano-emulsion filtration sterilization prepared, obtain purpose DHA nano-emulsion.
6. the DHA nano-emulsion as according to any one of Claims 1-4 is being prepared with the application in the transparent and dairy beverage that DHA is active component.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410711815.0A CN105614638A (en) | 2014-11-28 | 2014-11-28 | DHA nanoemulsion, preparation method thereof and application of DHA nanoemulsion in healthy health-care beverages |
| PCT/CN2014/093203 WO2016082241A1 (en) | 2014-11-28 | 2014-12-06 | Dha nanoemulsion, preparation method therefor, and applications thereof in healthy drinks |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410711815.0A CN105614638A (en) | 2014-11-28 | 2014-11-28 | DHA nanoemulsion, preparation method thereof and application of DHA nanoemulsion in healthy health-care beverages |
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| Publication Number | Publication Date |
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| CN105614638A true CN105614638A (en) | 2016-06-01 |
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| CN201410711815.0A Withdrawn CN105614638A (en) | 2014-11-28 | 2014-11-28 | DHA nanoemulsion, preparation method thereof and application of DHA nanoemulsion in healthy health-care beverages |
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| WO (1) | WO2016082241A1 (en) |
Citations (5)
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Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010008475A2 (en) * | 2008-06-23 | 2010-01-21 | Virun, Inc. | Compositions containing nono-polar compounds |
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2014
- 2014-11-28 CN CN201410711815.0A patent/CN105614638A/en not_active Withdrawn
- 2014-12-06 WO PCT/CN2014/093203 patent/WO2016082241A1/en active Application Filing
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| CN102423297A (en) * | 2011-12-23 | 2012-04-25 | 厦门金达威集团股份有限公司 | Self-microemulsion and preparation method thereof |
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| WO2016082241A1 (en) | 2016-06-02 |
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Application publication date: 20160601 |