CN105613288B - A kind of construction method of Phnom Penh Chinese littleleaf box rapid propagation system - Google Patents
A kind of construction method of Phnom Penh Chinese littleleaf box rapid propagation system Download PDFInfo
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- CN105613288B CN105613288B CN201510999046.3A CN201510999046A CN105613288B CN 105613288 B CN105613288 B CN 105613288B CN 201510999046 A CN201510999046 A CN 201510999046A CN 105613288 B CN105613288 B CN 105613288B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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Abstract
The invention belongs to field of plant cell engineering technology, disclose a kind of method that tissue-culturing rapid propagation is carried out using golden-rimmed Chinese littleleaf box stem apex and stem section as explant.Stem section of this method to stem apex and with axillary bud is by inducing Multiple Buds, culture of rootage to obtain complete regenerated plant；For the stem section part without axillary bud successively by the induction of callus, the Proliferation, Differentiation of callus, culture of rootage, so as to obtain regeneration plant.This method can obtain regeneration plant by two kinds of approach, it is easy to operate, pollution rate is low in incubation, melting brown rate is low, a large amount of seedlings can not be obtained in a short time by season and environmental restrictions, the shortcomings that overcoming cutting propagation, at the same be also its genetic transformation, somaclonal variation breeding provides condition, great practical value is respectively provided in theoretical research and landscape application.
Description
Technical field
It a kind of is carried out the invention belongs to field of plant cell engineering technology more particularly to using golden-rimmed Chinese littleleaf box stem apex and stem section
The method of tissue-culturing rapid propagation.
Background technology
Golden-rimmed Chinese littleleaf box (Euonymus Japonicus cv.Aureo-ma), is Celastraceae burning buss, and alias is golden-rimmed
Euonymus japonicus, is one of mutation of Euonymus japonicus, and single leaf is to life, obovate or ellipse, edge tool cognate, surface bottle green,
Limb edge is golden yellow, glossy, very with ornamental value.Golden-rimmed Chinese littleleaf box light, slightly shade tolerant, it is adaptable, rudiment power and
Hair principal stresses is strong, and resistance to trimming is excellent Landscape Trees, has a large amount of plantations in each big city, park, colleges and universities and cell,
Seedling demand is big.Golden-rimmed Chinese littleleaf box is often bred at present in the method for cuttage, but cutting propagation is by limits such as season, materials
System, easy introduced disease during cuttage, and the plant service life obtained can shorten, and especially xylophyta cuttage there is also
The phenomenon that being preced with partially, so long-term cutting propagation can cause plant to degenerate, the age of tree shortens and a large amount of introduced diseases.
And carried out by tissue cultures fast numerous, it is not influenced by season environment not only, xylophyta growth week can be overcome
The shortcomings that phase is long, and can be that golden-rimmed Chinese littleleaf box somaclonal variation breeding, mutation breeding and genetic engineering breeding are established
Basis provides condition to create the strong new varieties of multicolour, resistance.On the other hand, axis nose part institute band virus
Seldom, a large amount of detoxic seedlings can be obtained by carrying out tissue cultures using stem apex, can reduce Pesticide use to a certain extent, not only
It is cost-effective to reduce air pollution, also to golden-rimmed Chinese littleleaf box germplasm pure keeping, rejuvenation important in inhibiting.
Invention content
Technical problem solved by the invention is that provide one kind carries out group using golden-rimmed Chinese littleleaf box stem apex and stem section as explant
The fast numerous method of training, this method can obtain golden-rimmed Chinese littleleaf box regeneration plant by two kinds of approach of callus and non-callus,
To industrial seedling rearing important in inhibiting.
Technical solution of the present invention is as follows：
A kind of Phnom Penh Chinese littleleaf box rapid propagation system construction method, this method carry out according to the following steps：
1) pretreatment and disinfection of explant
The current-year branch with the tender terminal bud of children is taken, then surrounding blade is cut off, is pre-processed and is sterilized, it is spare；
2) explant Initial culture and callus induction
The stem apex disinfected, the stem section with axillary bud, the stem section without axillary bud are inoculated into primary culture medium and carry out primary
Culture is generated evoked callus on callus inducing medium is inoculated into without the stem section of axillary bud；Condition of culture is light
Strong 800~1000lx, 16~18h/d of illumination, 25 DEG C of temperature；
3) induction of Multiple Buds and callus proliferation differentiation
A, the young sprout that 2~3cm long is grown in step 2) is cut, be transferred on inducing clumping bud culture medium, 30~45 days
Axillary bud, which is largely sprouted, forms Multiple Buds, and Multiple Buds are cut into multiple budlets clump every 4 weeks, be seeded on identical culture medium after
It is commissioned to train foster；In incubation, 2500~3000lx of intensity of illumination, 16~18h/d of illumination, 25~27 DEG C of temperature；
B, the callus of diameter 3cm or so sizes in step 2) is cut into 1cm fritters and is seeded in callus subculture
In culture medium, when the callus of subculture switch to it is emerald green can be inoculated on callus differential medium, induced synthesis is not
Normal bud；In incubation, 2500~3000lx of intensity of illumination, 16~18h/d of illumination, 25~27 DEG C of temperature；
4) culture of rootage
The healthy and strong young sprout that step 3) is grown into 2~3cm or so is cut, and is transferred on root media, is induced
It takes root, finally obtains complete regenerated plant, condition of culture is intensity of illumination 2000lx, illumination in 16 hours, 8 hours dark, temperature
25℃。
A kind of 2. construction method of golden-rimmed Chinese littleleaf box rapid propagation system according to claim 1, it is characterised in that step 1)
Explant preprocess method is to cut off blade to leave behind naked bub and naked stem, is impregnated 20~40 minutes in washing powder water, then flow
Water rinses 1~2 hour；Sterilization method is that explant is placed in 20~30s of immersion in 75% alcohol of mass fraction on super-clean bench,
Aseptic water washing one is to twice, then is placed in 0.1% mercuric chloride solution of 250mL mass fractions and impregnates 6 minutes, wherein in mercuric chloride solution
0.5~1mL Tween-80s need to be added in, the explant of disinfection will ensure to be submerged by mercuric chloride solution, and sterile water is used after mercuric chloride sanitized
It rinses 4~5 times, is finally blotted excessive moisture with aseptic filter paper.
Preferably, primary culture medium is in the step 2)：MS+6-BA2.0~3.0mg/L+NAA0.2~0.6mg/L
+ 20~30g/L sucrose+6~8g/L agar+0.1~0.2g/L activated carbons, Medium's PH Value are 5.8~6.0；Callus lures
Leading medium component is：MS+6-BA1.0~2.0mg/L+NAA0.5~1.0mg/L+30g/L sucrose+6g/L agar+0.1~
0.2g/L activated carbons, Medium's PH Value are 5.6~5.8；During callus induction, horizontal insertion culture medium is wanted without the stem section of axillary bud
In.
Preferably, inducing clumping bud culture medium is in the step 3)：MS+6-BA1.0mg/L+NAA0.05~
0.2mg/L+20~30g/L sucrose+6~8g/L agar, Medium's PH Value are 5.6~5.8；Callus proliferation medium is：
MS+6-BA2.0mg/L+NAA0.5~0.8mg/L+20~30g/L sucrose+6g/L agar, Medium's PH Value are 5.6~5.8；More
Injured tissue differential medium is：6-BA2.0~3.0mg/L+NAA0.1~0.5mg/L+20~30g/L+30g sucrose+6g/L fine jades
Fat, Medium's PH Value are 5.6~6.0.
Preferably, root media is in the step 4)：1/2MS+NAA0.1~0.5mg/L+IBA0.5~
1.0mg/L+20~30g/L sucrose+6g/L agar+0.2g/L activated carbons, Medium's PH Value are 5.6~6.0；The new slightly stem of inoculation
Section base portion will have notch, light culture be taken after inoculation 3 days.
Compared with the prior art, advantage is embodied in the present invention：
It is quickly bred 1. the present invention carries out golden-rimmed Chinese littleleaf box using the means of tissue cultures, it, can be with not by season environmental restrictions
The shortcomings that overcoming easy introduced disease during golden-rimmed Chinese littleleaf box seedling cutting propagation, and cutting propagation often makes the plant of acquisition
The phenomenon that service life can shorten, and xylophyta there is also inclined hat.
2nd, not only has a detoxification efficiency using small Shoot Tip Culture, and stem apex can direct seedling differentiation, growth cycle is short
(2 to 3 months can rooting and transplant survive), Phenotypic Observation aberration rate be low, so as to generate a large amount of disease-free and inhereditary features one
The seedling of cause.And test tube seedling is obtained by callus approach, it may occur that somaclonal variation, it is not isophenic so as to generate
Mutant can expand germ plasm resource, promote breeding of new variety.
3. this method can be regenerated by different explants, stock utilization is high, and hormone in medium proportioning is reasonable, examination
Pipe seedling germination rate is high, and growing way is vigorous.
4. the sterilization method that the present invention uses can take into account survival rate, pollution rate and melting brown rate, high germination rate can be reached,
Low stain rate and melting brown rate.
Description of the drawings
Fig. 1 is the method for the present invention flow chart；
Fig. 2 is the golden-rimmed Chinese littleleaf box Multiple Buds of the method for the present invention induction；
Fig. 3 is the golden-rimmed Chinese littleleaf box callus of the method for the present invention induction；
Fig. 4 is the golden-rimmed Chinese littleleaf box adventitious bud of the method for the present invention callus differentiation.
Fig. 5 is the golden-rimmed Chinese littleleaf box test tube seedling of the method for the present invention root induction.
Specific embodiment
Embodiment 1
1. explant pre-processes and disinfection：
The branch of robust growth, no disease and pests harm is chosen in nursery, chooses tender stem apex and the stem section conduct with axillary bud
Explant.Explant is removed into mature leaf, is impregnated 40 minutes in 4 DEG C of washing powder water, then is rinsed 1 hour with 4 DEG C of flowing water,
Explant is placed in 75% alcohol on super-clean bench and impregnates 30s, aseptic water washing one is to twice, then is placed in 4 DEG C of mass fractions
It is impregnated in 0.1% mercuric chloride solution, soaking time setting five gradients of 4min, 6min, 8min, 15min and 25min, wherein mercuric chloride
It needs to add in 1mL Tween-80s in solution, aseptic water washing 4~5 times is blotted excessive moisture with aseptic filter paper；It waits to be transferred to and just be commissioned to train
Support base culture.
In order to select most suitable pretreatment condition, the explant that different disinfecting times are handled is seeded in ingredient as MS+6-
On the culture medium of BA2.5mg/L+NAA0.5mg/L+30g/L sucrose+8g/L agar+0.2g/L activated carbons, Medium's PH Value is
5.6, condition of culture be light intensity 800lx, illumination 16h/d, 25 DEG C of temperature, after two weeks count melting brown rate, pollution rate, germination rate (table
1)。
Influence of the different mercuric chloride disinfecting times of table 1 to golden-rimmed Chinese littleleaf box terminal bud and stem segment with axillary bud Initial culture
From this result of the test it can be found that disinfection mercuric chloride sterilize 6 minutes can ensure high germination rate, low melting brown rate it is same
When, reach preferable bactericidal effect.
2. Initial culture：
Stem apex or the stem section with axillary bud are cut into the segment of 1~2cm on super-clean bench, morphology upper end is seeded in upwards
In primary culture medium；3~4 explants of every bottle of inoculation.It is 25 DEG C, light application time 18h/d to cultivate room temperature, intensity of illumination
1000lx；Primary culture based component be MS+6-BA2.5+NAA0.5mg/L+30g/L sucrose+8g/L agar, pH value 5.6, separately
Outside using stem segment with axillary bud as object, best golden-rimmed Chinese littleleaf box browning prevention and control measure (table 2) is probed by three kinds of processing；After inoculation 10 days
The terminal bud of stem apex or the axillary bud of stem section are sprouted, and 30~40 days young sprouts of culture can grow to 2~3cm；
The golden-rimmed Chinese littleleaf box tissue cultures He mushroom mode of table 2
Note：Material to be tested mercuric chloride disinfecting time is 6min.
This result of the test is understood：Primary culture based component for MS+6-BA1.0mg/L+NAA0.5mg/L+30g/L sucrose+
8g/L agar adds 0.2g/L AC, and pH value 5.6, melting brown rate is only 2.5%, and the melting brown rate for adding PVP is 17.6；Dark training
Melting brown rate is 22.6% under the conditions of supporting
3. induction and the squamous subculture of Multiple Buds：
When the explant of Initial culture newly slightly grows to 2~3cm, cut and be connected on inducing clumping bud culture medium,
30~45 days a large amount of axillary bud sproutings are simultaneously grown, every 4 weeks or so by big bud clump divide and the squamous subculture on identical culture medium, i.e.,
A large amount of Multiple Buds can be obtained；Inducing clumping bud culture medium is MS+6-BA1.0mg/L+NAA0.05mg/L+30g/L sucrose+7g/
L agar, Medium's PH Value 5.8, in incubation, intensity of illumination 3000lx, 16~18h/d of illumination, 25 DEG C of temperature；
4. culture of rootage：The test tube seedling for selecting 3~4cm high is transferred on root media, and dark treatment is placed on normally for 3 days
It is cultivated under photoperiod, the 25-32 days rooted seedlings that can obtain 5 or more more than root long 4cm, as shown in Figure 5；Root media is
1/2MS+NAA0.2mg/L+IBA0.5mg/L+20g/L sucrose+6g/L agar+0.2g/L activated carbons.Condition of culture is：Illumination is strong
Spend 2000lx, illumination 18h/d, 25 DEG C of temperature.Culture of rootage stage, the melting brown rate of tissue-cultured seedling are less than 2.5%.
Embodiment 2
1. explant pre-processes and disinfection：
The young tender stem section without axillary bud of golden-rimmed Chinese littleleaf box is chosen as explant, is impregnated 30 minutes in 4 DEG C of washing powder water, then
It is rinsed 1 hour with 4 DEG C of flowing water, explant is placed in 75% alcohol of mass fraction on super-clean bench and impregnates 30s, aseptic water washing
One to twice, then is placed in 4 DEG C of 0.1% mercuric chloride solution and impregnates, soaking time setting 5min, 8min, 12min, 15min, 25min
Five gradients wherein need to add in 1mL Tween-80s, aseptic water washing 4~5 times, with aseptic filter paper by excessive moisture in mercuric chloride solution
It blots.Explant is seeded in ingredient as MS+6-BA2.0+NAA0.5mg/L+30g/L sucrose+6g/L agar+0.2g/L activity
On the culture medium of charcoal.Condition of culture is the same as embodiment 1, two weeks statistics pollution rates, melting brown rate and healing rate.
The different mercuric chloride disinfecting times of table 3 are to influence of the golden-rimmed Chinese littleleaf box without axillary bud stem section Initial culture
By this experiment it is found that mercuric chloride disinfection 8min is golden-rimmed Chinese littleleaf box without the best disinfecting time of axillary bud stem section.
2. the induction of callus：
Stem section is cut into the segment of 1~2cm on super-clean bench, horizontal to be connected on callus inducing medium, every bottle of inoculation 3
~4 explants.It is 25 DEG C, light application time 18h/d, intensity of illumination 800lx to cultivate room temperature；Callus inducing medium into
It is divided into MS+6-BA2.0+NAA1.0mg/L+30g/L sucrose+6g/L agar+0.2g/L activated carbons, Medium's PH Value 5.6.3.
The subculture of callus and differentiation：
The callus lines for growing to diameter 3cm or so are cut into the fritter of diameter 1cm or so, are placed in callus proliferation training
Support base on, every 30 days or so in same medium subculture it is primary, subculture medium MS+6-BA2.0mg/L+NAA0.5mg/L
+ 30g/L sucrose+6g/L agar, Medium's PH Value 5.8；Callus can be switched to emerald green by yellow green during subculture, will
It is transferred in differential medium, may occur in which bud point within 20~35 days, is subsequently differentiate into adventitious bud, callus differential medium
For 6-BA2.5mg/L+NAA0.1mg/L+20~30g/L sucrose+6g/L agar, Medium's PH Value 5.8, condition of culture is：Light
According to intensity 2500lx, illumination illumination in 16 hours, 8 hours dark, 25 DEG C of temperature.
4. culture of rootage：
The young sprout for cutting 3cm or so robust growths is transferred on root media, and dark treatment after 3 days is transferred and trained under light again
It supports, culture obtains 4 or more the complete regrowths of more than root long 4cm in 3 weeks or so；Root media is 1/2MS+NAA0.2mg/L+
IBA0.5mg/L+20g/L sucrose+6g/L agar+0.2g/L activated carbons, Medium's PH Value 5.8.Condition of culture is：Illumination is strong
2000lx is spent, illumination 18h/d, 25 DEG C of temperature, in the culture of rootage stage, the melting brown rate of tissue-cultured seedling is less than 2.5%.
The regrowth of 2 gained of above-described embodiment 1 and embodiment can be obtained transplanted seedling to distinguish after conventional hardening
Transplanting.The transplanted seedling of embodiment 1 finds that the hereditary shape of phenotype and seedling keeps one substantially after strain through Phenotypic Observation
It causes, therefore a large amount of disease-free and consistent inhereditary feature seedlings can be generated.And the transplanted seedling obtained through 2 method of embodiment is in strain
It finds that aberration rate is higher by Phenotypic Observation, available for expanding germ plasm resource, promotes breeding of new variety.
Claims (2)
1. a kind of Phnom Penh Chinese littleleaf box rapid propagation system construction method, it is characterised in that this method carries out according to the following steps：
1）The pretreatment and disinfection of explant
The current-year branch with the tender terminal bud of children is taken, surrounding blade is cut off, is pre-processed and is sterilized, it is spare；
2）Explant Initial culture and callus induction
The stem apex disinfected, the stem section with axillary bud are inoculated into primary culture medium and carry out Initial culture, it will be without the stem of axillary bud
Section is inoculated into evoked callus on callus inducing medium and generates；Condition of culture is light intensity 800-1000lx, illumination 16-
18 h/d, 25 DEG C of temperature；
3）The induction of Multiple Buds and callus proliferation differentiation
A, by step 2）In grow into the young sprout of 2-3cm long and cut, be transferred on inducing clumping bud culture medium, 30-45 days axillary buds
A large amount of sprout forms Multiple Buds, and Multiple Buds are cut into multiple budlets clump every 4 weeks, is seeded on identical culture medium after being commissioned to train
It supports；In incubation, intensity of illumination 2500-3000 lx, illumination 16-18 h/d, 25-27 DEG C of temperature；
B, by step 2）The callus of middle diameter 3cm sizes cuts into 1cm fritters and is seeded in callus subculture medium,
When the callus of subculture switch to it is emerald green can be inoculated on callus differential medium, induced synthesis adventitious bud；Culture
In the process, intensity of illumination 2500-3000 lx, illumination 16-18 h/d, 25-27 DEG C of temperature；
4）Culture of rootage
By step 3）The healthy and strong young sprout for growing into 2-3cm or so is cut, and is transferred on root media, carries out root induction,
Final to obtain complete regenerated plant, condition of culture is intensity of illumination 2000lx, illumination in 16 hours, 8 hours dark, temperature 25
℃；
The step 2）Middle primary culture medium is：MS+6-BA2.0-3.0mg/L+NAA0.2-0.6mg/L+20-30g/L sucrose+
6-8g/L agar+0.1-0.2g/L activated carbons, Medium's PH Value 5.8-6.0；Callus inducing medium ingredient is：MS+
6-BA1.0-2.0mg/L+NAA0.5-1.0mg/L+30g/L sucrose+6g/L agar+0.1-0.2g/L activated carbons, Medium's PH Value
For 5.6-5.8；During callus induction, wanted in horizontal insertion culture medium without the stem section of axillary bud；
The step 3）Middle inducing clumping bud culture medium is：MS+6-BA1.0mg/L+NAA0.05-0.2mg/L+20-30 g/L
Sucrose+6-8 g/L agar, Medium's PH Value 5.6-5.8；Callus subculture medium is：MS+6-BA2.0mg/L+
NAA0.5-0.8mg/L+20-30 g/L sucrose+6g/L agar, Medium's PH Value 5.6-5.8；Callus differential medium
For：6-BA2.0-3.0mg/L+NAA0.1-0.5mg/L+20-30 g/L+30g sucrose+6g/L agar, Medium's PH Value are
5.6-6.0；
The step 4）Middle root media is：1/2MS+NAA0.1-0.5mg/L+IBA0.5-1.0mg/L+20-30 g/L sugarcanes
Sugar+6g/L agar+0.2g/L activated carbons, Medium's PH Value 5.6-6.0；The new slightly stem section base portion of inoculation will have notch, be inoculated with
Light culture is taken afterwards 3 days.
A kind of 2. construction method of golden-rimmed Chinese littleleaf box rapid propagation system according to claim 1, it is characterised in that step 1）Explant
Body preprocess method is that blade removing is left behind naked bub and naked stem, is impregnated 20-40 minutes in washing powder water, then flowing water rinses
1-2 hours；Sterilization method impregnates 20-30s for explant is placed in 75% alcohol of mass fraction on super-clean bench, sterile water punching
One is washed to twice, then is placed in 250 mL mass fractions, 0.1% mercuric chloride solution and impregnates 6 minutes, wherein needs to add in mercuric chloride solution
0.5-1mL Tween-80s are again finally inhaled excessive moisture with aseptic filter paper with aseptic water washing 4-5 times after mercuric chloride sanitized
It is dry.
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| CN106258973B (en) * | 2016-08-17 | 2018-08-24 | 北京林业大学 | A kind of red autumnal leaves winged euonymus polyploid cell breeding technique |
| CN106508680B (en) * | 2016-11-09 | 2018-07-10 | 河南红枫种苗股份有限公司 | A kind of culture medium and method of golden armor tissue cultures |
| CN110358790B (en) * | 2019-07-12 | 2023-02-24 | 河南科技学院 | Method for quickly genetically transforming or infecting plant with virus |
| CN112106656A (en) * | 2020-09-24 | 2020-12-22 | 安徽农业大学 | Direct organ generation type regeneration method of rhizoma acori graminei |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2055782A1 (en) * | 2007-10-30 | 2009-05-06 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Method for improving the crop of laticiferous plants |
| CN102293150A (en) * | 2010-06-22 | 2011-12-28 | 上海上房园艺有限公司 | Method for culturing tissues of Buxus sempervives |
| CN104969747A (en) * | 2015-06-18 | 2015-10-14 | 滁州市瑞景园林建设有限公司 | Method for rapidly cultivating spindle trees indoors |
-
2015
- 2015-12-24 CN CN201510999046.3A patent/CN105613288B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2055782A1 (en) * | 2007-10-30 | 2009-05-06 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Method for improving the crop of laticiferous plants |
| CN102293150A (en) * | 2010-06-22 | 2011-12-28 | 上海上房园艺有限公司 | Method for culturing tissues of Buxus sempervives |
| CN104969747A (en) * | 2015-06-18 | 2015-10-14 | 滁州市瑞景园林建设有限公司 | Method for rapidly cultivating spindle trees indoors |
Non-Patent Citations (3)
| Title |
|---|
| 大叶黄杨带腋芽茎段组织培养研究;吴广宇等;《安徽农业科学》;20051231;第33卷(第5期);第815,817页 * |
| 大叶黄杨无芽茎段不定芽再生及其起源;王茂良等;《林业科学》;20050531;第41卷(第3期);第179-181页，尤其是第179页第1节至第180页第2.1节 * |
| 金叶大叶黄杨离体培养技术研究;薛会雯等;《广东农业科学》;20121231(第3期);第46-48页，尤其是摘要，第46-47页第1.2节 * |
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