CN105606829A - Newborn blood type identification card - Google Patents
Newborn blood type identification card Download PDFInfo
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- CN105606829A CN105606829A CN201511010701.4A CN201511010701A CN105606829A CN 105606829 A CN105606829 A CN 105606829A CN 201511010701 A CN201511010701 A CN 201511010701A CN 105606829 A CN105606829 A CN 105606829A
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
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Abstract
The invention relates to a newborn blood type identification card, comprising a detection card with six micro-columns, wherein the six micro-columns are respectively filled with an equivalent quantity of anti-A gel, anti-B gel, anti-AB gel, anti-D gel, anti-human globule gel and blank gel. The newborn blood type identification card is formed according to the following steps of swelling sephadex gels; preparing all antibody diluent; preparing all antibody working solutions; preparing the gels; subpackaging. The newborn blood type identification card has the advantages that the sephadex gels of different sizes and different screening ranges are mixed, and are swelled by utilization of purified water, so that the specificity is effectively guaranteed while a higher sensitivity is also obtained, and the ionic strength of a system is effectively guaranteed, therefore important clinical significance for diagnosing, preventing and treating hemolytic disease of the newborn is realized.
Description
Technical field
The present invention relates to the medical domain of transfusing blood, relate in particular to a kind of neonate's blood group identification card.
Background technology
Neonatal hemolytic disease (HDN) refers to the blood group incompatibility due to mothers and sons, in mother's body, produce the blood group antibody being unworthy of with fetal blood group antigen, this antibody is entered into and in fetus body, is caused isoimmunization haemolysis by placenta, be common in the blood group incompatibility of ABO blood group system and Rh blood group system, therefore neonatal bracket for blood grouping is for the diagnosis important in inhibiting of neonatal hemolytic disease.
Neonate is because abiogenous antibody amount is less, and after major part will arrive birth, some months just can form blood group antibody, and conventionally also can have the antibody that derives from parent, and therefore neonatal abo blood group detects and is fixed to master, without carrying out reverse type.
Micro-column gel immunoassay technology is a kind of immunoassay technology producing the nineties in 20th century, its general principle is to utilize sieve technology, centrifugation technique and specific immune response know-why, blood group serology technology and gel molecular sieve technology are combined, can detect delicately the faint blood group antigens antibody response that may exist, be considered to the milestone of blood group serology inspection.
Have at present some manufacturer production neonate blood group identification cards both at home and abroad, but have following some shortcomings: the one, sephadex or the polypropylene sephadex of the single model of employing, cannot take into account sensitivity and specificity; The 2nd, adopt physiological saline or antibody buffer solution swell gel, make fully complete swelling of gel, and destroy the ionic strength of reaction system; The 3rd, to adding the gel of different antibodies, gel detergent buffer solution is in full accord, cannot effectively protect antibody stability; The 4th, still need to do reverse type test, increase operating personnel's work load.
Summary of the invention
The present invention will overcome above deficiency exactly, provide a kind of highly sensitive, Idiotype good, stability is strong, neonate's blood group identification card of long shelf-life.
For achieving the above object, the present invention solves by the following technical programs:
A kind of neonate's blood group identification card, comprises the test card that contains six microtrabeculaes, and the anti-A gel of equivalent, anti-B gel, anti-AB gel, anti-D gel, anti-human ball gel and blank gel are housed respectively in described six microtrabeculaes, and it is prepared from by the following method:
(1) sephadex is swelling: select at least two kinds of sephadexs to mix, then add and in purified water, carry out abundant suspension swelling, remove crushed particles with purified water washing again, obtain size evenly, be complete spherical sephadex particle;
(2) prepare each antibody diluent:
Anti-A dilution: taking physiological saline as base fluid, wherein contain EDTA Dipotassium salt (EDTA di-potassium) 10-15g/L, bovine serum albumin(BSA) 20-25g/L, sucrose 15-20g/L; The pH value of anti-A dilution is 6.6-6.8;
Anti-B dilution: taking physiological saline as base fluid, wherein contain EDTA Dipotassium salt 15-20g/L, bovine serum albumin(BSA) 15-20g/L, sucrose 25-30g/L, the pH value of anti-B dilution is 6.6-6.8;
Anti-AB dilution: taking physiological saline as base fluid, wherein contain EDTA Dipotassium salt 15-20g/L, bovine serum albumin(BSA) 10-15g/L, sucrose 18-26g/L, the pH value of dilution is 6.6-6.8;
Anti-D dilution: taking phosphate buffer (PBS) as base fluid, wherein contain EDTA Dipotassium salt 10-15g/L, bovine serum albumin(BSA) 35-40g/L, the pH value of dilution is 6.6-6.8;
Blank gel detergent buffer solution: taking purified water as solvent, contain sodium chloride 1.75g-2.07g/L, sodium hydrogen phosphate 0.185-0.219g/L, sodium dihydrogen phosphate 0.203-0.24g/L, glycine 18-21g/L in buffer solution; And regulate pH value for 6.6-6.8;
(3) prepare each antibody working solution:
Get anti-A monoclonal antibody concentrate and mix with anti-A dilution, make the tiring of the anti-A monoclonal antibody working solution that obtains >=128;
Get anti-B monoclonal antibody concentrate and mix with anti-B dilution, make the tiring of the anti-B monoclonal antibody working solution that obtains >=128;
Get anti-AB monoclonal antibody concentrate and mix with anti-AB dilution, make the tiring of the anti-AB monoclonal antibody working solution that obtains >=128;
Get anti-D monoclonal antibody concentrate and mix with anti-D dilution, make the tiring of the anti-D monoclonal antibody working solution that obtains >=64;
Choose tire >=32 broad spectrum antihumanglobulin reagent of the red blood cell of anti-D immunoglobulin (IgG) sensitization;
(4) prepare gel:
The sephadex particle of getting six parts of equivalent washs at least three times with the anti-A dilution of equivalent, anti-B dilution, anti-AB dilution, anti-D dilution, blank gel detergent buffer solution and blank gel detergent buffer solution respectively, add again corresponding anti-A monoclonal antibody working solution, anti-B monoclonal antibody working solution, anti-AB monoclonal antibody working solution, anti-D monoclonal antibody working solution, broad spectrum antihumanglobulin reagent and blank gel detergent buffer solution to mix, obtain anti-A gel, anti-B gel, anti-AB gel, anti-D gel, anti-human ball gel and blank gel;
(5) packing:
According to the amount of every pipe 24-32 microlitre, will resist A gel, anti-B gel, anti-AB gel, anti-D gel, anti-human ball gel and blank gel to be filled into respectively in six microtrabeculaes, form neonate's blood group identification card.
Further scheme, in described step (1), sephadex is sephadex G-25, sephadex G-50, sephadex G-75 or sephadex G-100.
Further scheme, carries out abundant suspension swelling in adding purified water in described step (1) in sephadex mixture, and wherein the mass volume ratio of sephadex mixture and purified water is 1:2-1:3.
Further scheme, in described step (1), the time of suspension swelling is 48-72 hour.
Further scheme, in described step (4) anti-A monoclonal antibody working solution, anti-B monoclonal antibody working solution, anti-AB monoclonal antibody working solution, anti-D monoclonal antibody working solution, broad spectrum antihumanglobulin reagent, blank gel detergent buffer solution respectively with sephadex particle after washing by volume for 1:2-1:3 mixes.
The present invention selects six equal portions by the sephadex particle that carries out swelling gained in purified water at least two kinds of sephadexs, use respectively more anti-A dilution, anti-B dilution, anti-AB dilution, anti-D dilution, blank gel detergent buffer solution and blank gel detergent buffer solution wash at least three times it respectively, add again corresponding anti-A monoclonal antibody working solution, anti-B monoclonal antibody working solution, anti-AB monoclonal antibody working solution, anti-D monoclonal antibody work, broad spectrum antihumanglobulin reagent and blank gel detergent buffer solution mix, obtain anti-A gel, anti-B gel, anti-AB gel, anti-D gel, anti-human ball gel and blank gel. again they are packed into successively in six microtrabeculaes in test card, form neonate's blood group identification card. this test card is used for detecting neonatal bracket for blood grouping, diagnoses, prevents and treat to have important clinical meaning thereby realize for neonatal hemolytic disease.
So advantage of the present invention is:
1, the present invention adopts the sephadex of different sizes, different screening scopes to mix, the specificity of single sephadex and the feature that sensitivity can not take into account are effectively avoided, can realize ensureing the specific while, still have very high sensitivity;
2, the present invention adopts the swelling solution of purified water as sephadex, can make gel particle swelling cmpletely;
3, for the present invention, each antibody diluent washs sephadex particle, thereby can not destroy the protection liquid composition of antibody, the stability of the more effective guarantee antibody of energy; And ensured the pure property of buffer solution system not to be subject to the impact of other ions, effectively ensure the ionic strength of system.
4, the present invention, without carrying out the test of neonate ABO reverse type, can alleviate medical personnel and operate burden, and gets rid of the antibody that derives from parent for the interference of test.
Concrete implementing method
Embodiment mono-:
Neonate's blood group identification card comprises a test card that contains six microtrabeculaes, and the anti-A gel of equivalent, anti-B gel, anti-AB gel, anti-D gel, anti-human ball gel and blank gel are housed respectively in described six microtrabeculaes, and it is prepared from by the following method:
(1) sephadex is swelling:
Select sephadex G-25, sephadex G-50 and sephadex G-75 to be mixed to get sephadex mixture according to mass ratio 1:2:1, then be that 1:3 adds purified water according to the mass volume ratio of sephadex mixture and purified water, carry out suspension swelling after 48 hours; Again by purified water to its washing three times, remove crushed particles, obtain size evenly, be complete spherical solidifying glucan glue particle;
(2) preparation of antibody diluent:
Anti-A dilution: taking physiological saline as base fluid, wherein contain EDTA Dipotassium salt (EDTA di-potassium) 10g/L, bovine serum albumin(BSA) 20g/L, sucrose 20g/L, the pH value of dilution is 6.6-6.8;
Anti-B dilution: taking physiological saline as base fluid, wherein contain EDTA Dipotassium salt (EDTA di-potassium) 15g/L, bovine serum albumin(BSA) 15g/L, sucrose 30g/L, the pH value of dilution is 6.6-6.8;
Anti-AB dilution: taking physiological saline as base fluid, wherein contain EDTA Dipotassium salt (EDTA di-potassium) 16g/L, bovine serum albumin(BSA) 12g/L, sucrose 20g/L, the pH value of dilution is 6.6-6.8;
Anti-D dilution: taking phosphate buffer (PBS) as base fluid, wherein contain EDTA Dipotassium salt (EDTA di-potassium) 15g/L, bovine serum albumin(BSA) 40g/L, the pH value of dilution is 6.6-6.8;
Blank gel detergent buffer solution: taking purified water as solvent, contain sodium chloride 1.75gg/L, sodium hydrogen phosphate 0.185g/L, sodium dihydrogen phosphate 0.203g/L, glycine 18g/L in buffer solution; And regulate pH value for 6.6-6.8;
(3) prepare each antibody working solution:
To get anti-A dilution prepared by anti-A monoclonal antibody concentrate and step (2) and be mixed to get anti-A monoclonal antibody working solution, its anti-A monoclonal antibody working solution tires >=and 128;
To get anti-B dilution prepared by anti-B monoclonal antibody concentrate and step (2) and be mixed to get anti-B monoclonal antibody working solution, its anti-B monoclonal antibody working solution tires >=and 128;
To get anti-AB dilution prepared by anti-AB monoclonal antibody concentrate and step (2) and be mixed to get anti-AB monoclonal antibody working solution, its anti-AB monoclonal antibody working solution tires >=and 128;
To get anti-D dilution prepared by anti-D monoclonal antibody concentrate and step (2) and be mixed to get anti-D monoclonal antibody working solution, its anti-D monoclonal antibody working solution tires >=and 64;
Choose tire >=32 broad spectrum antihumanglobulin reagent of the red blood cell of anti-D immunoglobulin (IgG) sensitization;
(4) prepare gel:
Sephadex particle swelling in step (1) is divided into six equal portions, a copy of it washs three times with the anti-A dilution of equivalent, mix according to volume ratio 1:2 with the sephadex particle after washing with anti-A monoclonal antibody working solution again, obtain anti-A gel;
Second part of washing of the anti-B dilution by equivalent three times, then with anti-B monoclonal antibody working solution with wash after sephadex particle mix according to volume ratio 1:2 ratio, obtain anti-B gel;
The 3rd part of washing of the anti-AB dilution by equivalent three times, then with anti-AB monoclonal antibody working solution with wash after sephadex particle mix according to volume ratio 1:2 ratio, obtain anti-AB gel;
The 4th part with the anti-D dilution of equivalent washing three times, then with anti-D monoclonal antibody working solution with wash after sephadex particle mix according to volume ratio 1:2 ratio, obtain anti-D gel;
The 5th part with the blank gel detergent buffer solution washing of equivalent three times, then with broad spectrum antihumanglobulin reagent with wash after sephadex particle mix according to volume ratio 1:2 ratio, obtain anti-human ball gel;
The 6th part with the blank gel detergent buffer solution washing of equivalent three times, then with blank gel detergent buffer solution with wash after sephadex particle mix according to volume ratio 1:2 ratio, obtain blank gel;
(5) packing:
According to the amount of every pipe 32 microlitres, each gel of preparation in step (4) is joined in six microtrabeculaes in blank test card successively, wherein first microtrabeculae adds anti-A gel, second microtrabeculae adds anti-B gel, the 3rd microtrabeculae adds anti-AB gel, and the 4th microtrabeculae adds anti-D gel, and the 5th microtrabeculae adds anti-human ball gel, the 6th microtrabeculae adds blank gel, forms neonate's blood group identification card.
Embodiment bis-:
A kind of neonate's blood group identification card comprises the test card that contains six microtrabeculaes, in described six microtrabeculaes, first three microtrabeculae is equipped with respectively the anti-A gel of equivalent, anti-B gel and anti-D gel, its excess-three microtrabeculae is equipped with the blank gel of equivalent, and it is prepared from by the following method:
(1) sephadex is swelling:
Select sephadex G-75 and sephadex G-100 to be mixed to get sephadex mixture according to mass ratio 1:1, then be that 1:2 adds purified water according to the mass volume ratio of sephadex mixture and purified water, carry out suspension swelling after 48 hours; Again by purified water to its washing three times, remove crushed particles, obtain size evenly, be complete spherical solidifying glucan glue particle;
(2) preparation of antibody diluent:
Anti-A dilution: taking physiological saline as base fluid, wherein contain EDTA Dipotassium salt (EDTA di-potassium) 15g/L, bovine serum albumin(BSA) 22g/L, sucrose 18g/L, the pH value of dilution is 6.6-6.8;
Anti-B dilution: taking physiological saline as base fluid, wherein contain EDTA Dipotassium salt (EDTA di-potassium) 18g/L, bovine serum albumin(BSA) 20g/L, sucrose 25g/L, the pH value of dilution is 6.6-6.8;
Anti-AB dilution: taking physiological saline as base fluid, wherein contain EDTA Dipotassium salt (EDTA di-potassium) 19g/L, bovine serum albumin(BSA) 15g/L, sucrose 25g/L, the pH value of dilution is 6.6-6.8;
Anti-D dilution: taking phosphate buffer (PBS) as base fluid, wherein contain EDTA Dipotassium salt (EDTA di-potassium) 12g/L, bovine serum albumin(BSA) 35g/L, the pH value of dilution is 6.6-6.8;
Blank gel detergent buffer solution: taking purified water as solvent, contain sodium chloride 1.8g/L, sodium hydrogen phosphate 0.2g/L, sodium dihydrogen phosphate 0.22g/L, glycine 20g/L in buffer solution; And regulate pH value for 6.6-6.8;
(3) prepare each antibody working solution:
Get anti-A dilution in anti-A monoclonal antibody concentrate and step (2) and be mixed to get anti-A monoclonal antibody working solution, wherein anti-A monoclonal antibody working solution tire >=128;
Get anti-B dilution in anti-B monoclonal antibody concentrate and step (2) and be mixed to get anti-B monoclonal antibody working solution, wherein anti-B monoclonal antibody working solution tire >=128;
Get anti-AB dilution in anti-AB monoclonal antibody concentrate and step (2) and be mixed to get anti-AB monoclonal antibody working solution, wherein anti-AB monoclonal antibody working solution tire >=128;
Get anti-D dilution in anti-D monoclonal antibody concentrate and step (2) and be mixed to get anti-D monoclonal antibody working solution, wherein anti-D monoclonal antibody working solution tire >=64;
Choose tire >=32 broad spectrum antihumanglobulin reagent of the red blood cell of anti-D immunoglobulin (IgG) sensitization;
(4) prepare gel:
Sephadex particle swelling in step (1) is divided into six equal portions, a copy of it washs three times with the anti-A dilution of equivalent, mix according to volume ratio 1:3 with the sephadex particle after washing with anti-A monoclonal antibody working solution again, obtain anti-A gel;
Second part of washing of the anti-B dilution by equivalent three times, then with anti-B monoclonal antibody working solution with wash after sephadex particle mix according to volume ratio 1:3 ratio, obtain anti-B gel;
The 3rd part of washing of the anti-AB dilution by equivalent three times, then with anti-AB monoclonal antibody working solution with wash after sephadex particle mix according to volume ratio 1:3 ratio, obtain anti-AB gel;
The 4th part with the anti-D dilution of equivalent washing three times, then with anti-D monoclonal antibody working solution with wash after sephadex particle mix according to volume ratio 1:3 ratio, obtain anti-D gel;
The 5th part with the blank gel detergent buffer solution washing of equivalent three times, then with broad spectrum antihumanglobulin reagent with wash after sephadex particle mix according to volume ratio 1:3 ratio, obtain anti-human ball gel;
The 6th part with the blank gel detergent buffer solution washing of equivalent three times, then with blank gel detergent buffer solution with wash after sephadex particle mix according to volume ratio 1:3 ratio, obtain blank gel;
(5) packing:
According to the amount of every pipe 24 microlitres, wherein first microtrabeculae adds anti-A gel, and second microtrabeculae adds anti-B gel, the 3rd microtrabeculae adds anti-AB gel, and the 4th microtrabeculae adds anti-D gel, and the 5th microtrabeculae adds anti-human ball gel, the 6th microtrabeculae adds blank gel, forms neonate's blood group identification card.
Above-described is only the preferred embodiment of the present invention, it should be pointed out that for the person of ordinary skill of the art, without departing from the concept of the premise of the invention, can also make some distortion and improvement, and these all belong to protection scope of the present invention.
Claims (5)
1. neonate's blood group identification card, comprise the test card that contains six microtrabeculaes, the anti-A gel of equivalent, anti-B gel, anti-AB gel, anti-D gel, anti-human ball gel and blank gel are housed respectively in described six microtrabeculaes, it is characterized in that: it is prepared from by the following method:
(1) sephadex is swelling: select at least two kinds of sephadexs to mix, then add and in purified water, carry out abundant suspension swelling, remove crushed particles with purified water washing again, obtain size evenly, be complete spherical sephadex particle;
(2) prepare each antibody diluent:
Anti-A dilution: taking physiological saline as base fluid, wherein contain EDTA Dipotassium salt (EDTA di-potassium) 10-15g/L, bovine serum albumin(BSA) 20-25g/L, sucrose 15-20g/L; The pH value of anti-A dilution is 6.6-6.8;
Anti-B dilution: taking physiological saline as base fluid, wherein contain EDTA Dipotassium salt 15-20g/L, bovine serum albumin(BSA) 15-20g/L, sucrose 25-30g/L, the pH value of anti-B dilution is 6.6-6.8;
Anti-AB dilution: taking physiological saline as base fluid, wherein contain EDTA Dipotassium salt 15-20g/L, bovine serum albumin(BSA) 10-15g/L, sucrose 18-26g/L, the pH value of dilution is 6.6-6.8;
Anti-D dilution: taking phosphate buffer (PBS) as base fluid, wherein contain EDTA Dipotassium salt 10-15g/L, bovine serum albumin(BSA) 35-40g/L, the pH value of dilution is 6.6-6.8;
Blank gel detergent buffer solution: taking purified water as solvent, contain sodium chloride 1.75g-2.07g/L, sodium hydrogen phosphate 0.185-0.219g/L, sodium dihydrogen phosphate 0.203-0.24g/L, glycine 18-21g/L in buffer solution; And regulate pH value for 6.6-6.8;
(3) prepare each antibody working solution:
Get anti-A monoclonal antibody concentrate and mix with anti-A dilution, make the tiring of the anti-A monoclonal antibody working solution that obtains >=128;
Get anti-B monoclonal antibody concentrate and mix with anti-B dilution, make the tiring of the anti-B monoclonal antibody working solution that obtains >=128;
Get anti-AB monoclonal antibody concentrate and mix with anti-AB dilution, make the tiring of the anti-AB monoclonal antibody working solution that obtains >=128;
Get anti-D monoclonal antibody concentrate and mix with anti-D dilution, make the tiring of the anti-D monoclonal antibody working solution that obtains >=64;
Choose tire >=32 broad spectrum antihumanglobulin reagent of the red blood cell of anti-D immunoglobulin (IgG) sensitization;
(4) prepare gel:
The sephadex particle of getting six parts of equivalent washs at least three times with the anti-A dilution of equivalent, anti-B dilution, anti-AB dilution, anti-D dilution, blank gel detergent buffer solution and blank gel detergent buffer solution respectively, add again corresponding anti-A monoclonal antibody working solution, anti-B monoclonal antibody working solution, anti-AB monoclonal antibody working solution, anti-D monoclonal antibody working solution, broad spectrum antihumanglobulin reagent and blank gel detergent buffer solution to mix, obtain anti-A gel, anti-B gel, anti-AB gel, anti-D gel, anti-human ball gel and blank gel;
(5) packing:
According to the amount of every pipe 24-32 microlitre, will resist A gel, anti-B gel, anti-AB gel, anti-D gel, anti-human ball gel and blank gel to be filled into respectively in six microtrabeculaes, form neonate's blood group identification card.
2. neonate's blood group identification card according to claim 1, is characterized in that: in described step (1), sephadex is sephadex G-25, sephadex G-50, sephadex G-75 or sephadex G-100.
3. neonate's blood group identification card according to claim 1, it is characterized in that: in adding purified water in described step (1) in sephadex mixture, carry out abundant suspension swelling, wherein the mass volume ratio of sephadex mixture and purified water is 1:2-1:3.
4. neonate's blood group identification card according to claim 1, is characterized in that: in described step (1), the time of suspension swelling is 48-72 hour.
5. neonate's blood group identification card according to claim 1, is characterized in that: in described step (4) anti-A monoclonal antibody working solution, anti-B monoclonal antibody working solution, anti-AB monoclonal antibody working solution, anti-D monoclonal antibody working solution, broad spectrum antihumanglobulin reagent, blank gel detergent buffer solution respectively with washing after sephadex particle by volume for 1:2-1:3 mixes.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511010701.4A CN105606829A (en) | 2015-12-30 | 2015-12-30 | Newborn blood type identification card |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511010701.4A CN105606829A (en) | 2015-12-30 | 2015-12-30 | Newborn blood type identification card |
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| CN105606829A true CN105606829A (en) | 2016-05-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201511010701.4A Pending CN105606829A (en) | 2015-12-30 | 2015-12-30 | Newborn blood type identification card |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106970233A (en) * | 2017-05-02 | 2017-07-21 | 临沂大学 | A kind of newborn mule coltfoal kit for testing hemolytic disease and preparation method thereof |
| CN112684176A (en) * | 2020-12-04 | 2021-04-20 | 上海润普生物技术有限公司 | Rh blood group antigen detection card and preparation method thereof |
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| CN101718783A (en) * | 2009-11-25 | 2010-06-02 | 江阴力博医药生物技术有限公司 | Method for preparing broad spectrum antihumanglobulin cards |
| CN102507961A (en) * | 2011-10-21 | 2012-06-20 | 江苏中盛医学诊断试剂有限公司 | Newborn ABO/Rh blood grouping reagent card and preparation method thereof |
| CN104569443A (en) * | 2014-12-22 | 2015-04-29 | 合肥天一生物技术研究所 | Rh blood type detecting card |
| CN104569444A (en) * | 2014-12-22 | 2015-04-29 | 合肥天一生物技术研究所 | Forward and reverse ABO typing and RhD blood type detecting card |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101718783A (en) * | 2009-11-25 | 2010-06-02 | 江阴力博医药生物技术有限公司 | Method for preparing broad spectrum antihumanglobulin cards |
| CN102507961A (en) * | 2011-10-21 | 2012-06-20 | 江苏中盛医学诊断试剂有限公司 | Newborn ABO/Rh blood grouping reagent card and preparation method thereof |
| CN104569443A (en) * | 2014-12-22 | 2015-04-29 | 合肥天一生物技术研究所 | Rh blood type detecting card |
| CN104569444A (en) * | 2014-12-22 | 2015-04-29 | 合肥天一生物技术研究所 | Forward and reverse ABO typing and RhD blood type detecting card |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106970233A (en) * | 2017-05-02 | 2017-07-21 | 临沂大学 | A kind of newborn mule coltfoal kit for testing hemolytic disease and preparation method thereof |
| CN106970233B (en) * | 2017-05-02 | 2019-06-14 | 临沂大学 | A kind of new life mule coltfoal kit for testing hemolytic disease and preparation method thereof |
| CN112684176A (en) * | 2020-12-04 | 2021-04-20 | 上海润普生物技术有限公司 | Rh blood group antigen detection card and preparation method thereof |
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