CN105606573B - A kind of System and method for of early diagnosis diagnosis - Google Patents
A kind of System and method for of early diagnosis diagnosis Download PDFInfo
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- CN105606573B CN105606573B CN201510967267.2A CN201510967267A CN105606573B CN 105606573 B CN105606573 B CN 105606573B CN 201510967267 A CN201510967267 A CN 201510967267A CN 105606573 B CN105606573 B CN 105606573B
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- 238000013399 early diagnosis Methods 0.000 title claims abstract description 16
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- 238000010186 staining Methods 0.000 claims description 14
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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Abstract
The present invention is suitable for medical information technical field, provides a kind of System and method for of early diagnosis diagnosis, the system comprises: sample preparation module, optical imagery module and data analysis module；The sample preparation module first extracts lesion sample tissue from patient's body, then using the fixed lesion sample tissue of chemical reagent, then carries out light transparent processing to lesion sample tissue using light clarifier, finally carry out fluorescent staining processing to lesion sample tissue；The lesion sample tissue that the optical imagery module is first placed and fixed fluorescent staining is handled, optical scanner imaging is carried out to the lesion sample tissue that fluorescent staining is handled is carried out using functional form micro imaging system again, obtains the functional diagram and optical section figure of lesion sample tissue；The data analysis module compares and analyzes the functional diagram and optical section figure with existing illness database data, generates diagnostic result report.The present invention, film-making speed block and slice-making quality are high.
Description
Technical field
The invention belongs to the System and method fors that medical information technical field more particularly to a kind of early diagnosis diagnose.
Background technique
Pathological diagnosis is diagnosis most authoritative in current modern medicine, it refers to the needs for answering Clinics and Practices, from trouble
Pathological tissues are taken out in person's body, carry out the technology of pathologic finding.By analysis, the identification to pathological tissues and cellular morphology, then
In conjunction with visually observing and clinically relevant data, the diagnosis of various diseases is made.
Pathological diagnosis in art just can determine that lesion is good pernicious, can be according to diagnostic result because its required time is short in art
Clinic formulates further operation plan and provides foundation, is increasingly subject to the welcome of domestic and international various big hospital clinical operation department.In art
Pathological diagnosis is exactly that unknown to property lesion in operation carries out is made a definite diagnosis, if being diagnosed as malignant tumour, so that it may straight
It connects and modus operandi and range is selected according to result, optimize operation plan, to be effectively prevented from second operation and damage.State at present
In the art that inside and outside various big hospital generally uses there are mainly three types of pathological diagnosis methods: (1) in art quick frozen-section pathologic finding,
It is will the pathological tissue quick film-making in the frozen state that cut off in operation, after conventional rapid dyeing for Pathology Doctors ' into
Row rapid pathological diagnosis, then report result is issued into clinic.Pathology Doctors ' generally does bid being connected to sample 30-50 minutes or so
This quick diagnosis.But it above has a certain limitation in application, and organizing cannot be too small, is unable to mass simultaneous and provides pathology to examine
It is disconnected as a result, and frozen section be easy to appear ice crystal, phenomena such as cellular swelling deforms.(2) routine paraffin wax is sliced pathologic finding, answers
It is wide with range, it is pathology excellent diagnostics, but the film-making time is very long, makes pathological diagnosis and need 3-5 days, much can not meet clinic
Demand with patient to early diagnosis early treatment.(3) quick paraffin sections pathologic finding, it is to allow sample group by high-temperature heating
It knits and is completed in a short time fast dewatering and waxdip process, principle is similar to routine paraffin wax slice pathologic finding to effect.Root
The factors such as the diagnostic level according to materials, production effect and Pathology Doctors ', it needs can issue report within -24 hours 50 minutes.
Although chipping qualities is sliced no less than routine paraffin wax, due to simplifying the manufacturing process of routine paraffin wax slice, film-making matter
Amount is difficult to control.
Summary of the invention
In view of this, the embodiment of the present invention provides a kind of System and method for of early diagnosis diagnosis, it is existing to solve
The problem of method of pathological diagnosis in the art that technology provides, film-making speed is slow and slice-making quality is difficult control.
In a first aspect, providing a kind of system of early diagnosis diagnosis, which is characterized in that the system comprises: sample
Prepare module, optical imagery module and data analysis module；
The sample preparation module includes:
Tissue collecting's unit, for extracting lesion sample tissue from patient's body；
Fixed cell is organized, the lesion sample tissue for extracting using the fixed tissue collecting's unit of chemical reagent；
Organize light transparent cell, for using the light clarifier lesion sample tissue fixed to the tissue fixed cell into
Row light transparent processing；
Tissue staining unit, for carrying out the lesion sample tissue that light transparent processing obtains to the tissue light transparent cell
Carry out fluorescent staining processing；
The optical imagery module includes:
Sample placement unit carries out the lesion that fluorescent staining is handled for placing and fixing the tissue staining unit
Sample tissue；
Scanning and imaging unit, for carrying out fluorescent staining to the tissue staining unit using functional form micro imaging system
It handles obtained lesion sample tissue and carries out optical scanner imaging, obtain the functional diagram and optical section of lesion sample tissue
Figure；
The data analysis module, the functional diagram that the scanning and imaging unit is obtained and optical section figure and existing illness
Database data compares and analyzes, and generates diagnostic result report.
Further, the tissue light transparent cell lesion sample tissue fixed to the tissue fixed cell carries out light
Transparent processing specifically: the refractive index changed in lesion sample tissue mismatches, and increases light penetrating in lesion sample tissue
Power.
Further, the scanning and imaging unit includes:
First imaging subelement, for passing through lateral two-dimensional scanning of the functional form micro imaging system to lesion sample tissue
Obtain the functional diagram of lesion sample tissue；
Second imaging subelement, for being obtained by longitudinal scanning of the functional form micro imaging system to lesion sample tissue
The optical section figure of lesion sample tissue.
Further, the scanning and imaging unit further include:
Image storage and display unit, for storing and showing the first imaging subelement and the second imaging subelement scanning
The functional diagram and optical section figure of obtained lesion sample tissue；
Image processing unit, functional diagram and optical section figure for storing to described image storage and display unit count
Word processing.
Further, the data analysis module includes:
Pathogeny analytical unit is obtained for comparing the illness data in existing database with the optical imagery module scans
Functional diagram and optical section figure, infer disease source and evolutionary process；
Lesion nature analytical unit, for determining and analyzing lesion nature；
Diagnostic analysis unit provides diagnostic comments for carrying out medical diagnosis on disease analysis；
Report generation unit, for generating and sending diagnosis report.
Second aspect provides a kind of method of early diagnosis diagnosis, which comprises
Lesion sample tissue is extracted from patient's body；
Using the fixed lesion sample tissue of chemical reagent；
Light transparent processing is carried out to the fixed lesion sample tissue using light clarifier；
Fluorescent staining processing is carried out to the lesion sample tissue that light transparent processing obtains is carried out；
It places and fixes and carry out the lesion sample tissue that fluorescent staining is handled；
It is swept using functional form micro imaging system to the lesion sample tissue progress optics that fluorescent staining is handled is carried out
Imaging is retouched, the functional diagram and optical section figure of lesion sample tissue are obtained；
Functional diagram and optical section figure that scanning imagery is handled and existing illness database data are compared point
Analysis generates diagnostic result report.
Further, described specific to fixed lesion sample tissue progress light transparent processing using light clarifier
Are as follows: the refractive index changed in lesion sample tissue mismatches, and increases penetration power of the light in lesion sample tissue.
Further, the lesion sample group that progress fluorescent staining is handled using functional form micro imaging system
It knits and carries out optical scanner imaging, obtain the functional diagram and optical section figure of lesion sample tissue, comprising:
Lesion sample tissue is obtained to the lateral two-dimensional scanning of lesion sample tissue by functional form micro imaging system
Functional diagram；
The optics of lesion sample tissue is obtained by longitudinal scanning of the functional form micro imaging system to lesion sample tissue
Slice map.
Further, in the lesion sample handled using functional form micro imaging system progress fluorescent staining
Tissue carries out optical scanner imaging, after obtaining the functional diagram and optical section figure of lesion sample tissue, further includes:
Store and show the functional diagram and optical section figure of the lesion sample tissue that scanning obtains；
Digital processing is carried out to the functional diagram and optical section figure.
Further, the functional diagram that scanning imagery is handled and optical section figure and existing illness database number
According to comparing and analyzing, diagnostic result report is generated, comprising:
The functional diagram and optics that illness data in comparison existing database are obtained with the optical imagery module scans are cut
Piece figure infers disease source and evolutionary process；
It determines and analyzes lesion nature；
Medical diagnosis on disease analysis is carried out, diagnostic comments are provided；
Generate and send diagnosis report.
In the embodiment of the present invention, lesion sample tissue is fixed by sample preparation module, light is transparent, fluorescent staining
After processing, then the lesion sample tissue progress optical imaging scanners by optical imagery module prepared by sample preparation module, it can
To obtain the functional diagram and optical section figure of lesion sample tissue.It is this to pass through introducing light transparent technology and optical image technology phase
In conjunction with mode, the optical section function of similar actual slice may be implemented, further avoid actual slice it is occupied when
Between.Also, since there is no actual slice, lesion sample tissue form and structure save complete, optical imaging effect clarity
The effect that pathologic finding can be sliced with routine paraffin wax compares favourably, and provides accurate diagnostic data for doctor.In addition, and mesh
Pathologic finding-quick frozen-section pathologic finding is compared in the most art of preceding application, quick disease in a kind of art provided by the invention
Diagnostic system is managed, 60 minutes quick diagnosis theoretically most may be implemented fastly.
Detailed description of the invention
Fig. 1 is the structural block diagram of the system embodiment of early diagnosis diagnosis of the present invention；
Fig. 2 is the implementation flow chart of the embodiment of the method for early diagnosis diagnosis of the present invention.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
In embodiments of the present invention, provide early diagnosis diagnosis system include: sample preparation module, optics at
As module and data analysis module；The sample preparation module includes: tissue collecting's unit, for extracting lesion from patient's body
Sample tissue；Fixed cell is organized, the lesion sample tissue for extracting using the fixed tissue collecting's unit of chemical reagent；
Light transparent cell is organized, it is transparent for carrying out light using the light clarifier lesion sample tissue fixed to the tissue fixed cell
Processing；Tissue staining unit, for the tissue light transparent cell carry out the obtained lesion sample tissue of light transparent processing into
Row fluorescent staining processing；The optical imagery module includes: sample placement unit, for placing and fixing the tissue staining list
Member carries out the lesion sample tissue that fluorescent staining is handled；Scanning and imaging unit, for using functional form micro imaging system
The lesion sample tissue that fluorescent staining is handled is carried out to the tissue staining unit and carries out optical scanner imaging, is obtained
The functional diagram and optical section figure of lesion sample tissue；The data analysis module, the function that the scanning and imaging unit is obtained
It can scheme and optical section figure is compared and analyzed with existing illness database data, generate diagnostic result report.
Realization of the invention is described in detail below in conjunction with specific embodiment:
Embodiment one
Fig. 1 shows the specific block diagram of the system of the early diagnosis diagnosis of the offer of the embodiment of the present invention one,
For ease of description, only parts related to embodiments of the present invention are shown.The early diagnosis diagnosis system 1 include:
Sample preparation module 11, optical imagery module 12 and data analysis module 13；
Wherein, the sample preparation module 11 includes:
Tissue collecting's unit 111, for extracting lesion sample tissue from patient's body；
Fixed cell 112 is organized, the lesion sample group for extracting using the fixed tissue collecting's unit of chemical reagent
It knits；
Light transparent cell 113 is organized, for the lesion sample group fixed to the tissue fixed cell using light clarifier
It knits and carries out light transparent processing；
Tissue staining unit 114, for carrying out the lesion sample that light transparent processing obtains to the tissue light transparent cell
Tissue carries out fluorescent staining processing.
Specifically, tissue collecting's unit 111 can extract lesion sample tissue from patient's body, it is subsequent to lesion sample
Tissue treatment provides must material.The operation of extraction includes but is not limited to cut, clamp and take, pierce through, removing.
Fixed lesion sample tissue refers to the component in quick solidification lesion sample tissue, and when keeping its living body
Structure can be conducive to subsequent to the transparent place of the light of lesion sample tissue after tissue fixed cell 112 fixes lesion sample tissue
Reason is handled with fluorescent staining.The chemical reagent of fixed lesion sample tissue includes but is not limited to formalin, formaldehyde, phosphoric acid buffer
Liquid etc. controls at 10 minutes or so the fixation used time of lesion sample tissue.
Light transparent processing refers to that the refractive index changed in lesion sample tissue mismatches, and increases light in lesion sample tissue
Penetration power, to lesion sample tissue carry out light transparent processing after, can be convenient subsequent optical image-forming module 12 to lesion sample
Tissue carries out the operation of profound optical section.Answering when hereafter lesion sample tissue no longer needs to carry out similar production paraffin section
Miscellaneous operation greatly reduces the time of sample production.To different lesion sample tissues can with specific aim using different formulations,
The clarifier of different ratio, can the case where according to lesion sample tissue such as the clarifier in number of patent application CN104568553A
The ratio of appropriate change fructose, polyethylene glycol, urea, glycerol etc..Exist to the light transparent processing used time control of lesion sample tissue
10 minutes or so.
Fluorescent staining processing to lesion sample tissue, can be convenient subsequent optical image-forming module 12 to lesion sample tissue
Carry out the operation of optical scanner imaging.The micro imaging system that the selection of fluorescent dye selects when can be imaged according to subsequent optical
Depending on, staining method includes but is not limited to fluorescence immunization coloration, fluorescent antibody staining etc..Fluorescent staining to lesion sample tissue
Used time control is handled at 10 minutes or so.
The optical imagery module 12 includes:
Sample placement unit 121 carries out what fluorescent staining was handled for placing and fixing the tissue staining unit
Lesion sample tissue；
Scanning and imaging unit 122, for carrying out fluorescence to the tissue staining unit using functional form micro imaging system
The lesion sample tissue that dyeing is handled carries out optical scanner imaging, obtains the functional diagram and optics of lesion sample tissue
Slice map.
Wherein, functional form micro imaging system includes but is not limited to confocal microscopic image system, two-photon micro-imaging
System, mating plate formula illumination micro imaging system, Structured Illumination micro imaging system etc..
Specifically, lesion sample tissue can be anchored on the load of functional form micro imaging system by sample placement unit 121
On object platform, during ensuring subsequent optical imaging, lesion sample tissue will not be moved.Wherein, the firm side of sample
Formula includes but is not limited to screw locking, spring leaf press etc..
Scanning and imaging unit 122 includes:
First imaging subelement, for passing through lateral two-dimensional scanning of the functional form micro imaging system to lesion sample tissue
Obtain the functional diagram of lesion sample tissue；
Second imaging subelement, for being obtained by longitudinal scanning of the functional form micro imaging system to lesion sample tissue
The optical section figure of lesion sample tissue.
Wherein, the selection of functional form micro imaging system determines that the mode of transversal scanning includes but is not limited to Structured Illumination
Face imaging, progressive scan, spiral scan etc., the precision of longitudinal scanning is according to the thickness of lesion sample tissue and slice thickness
Demand can be selected in 0.5 μm of -5 μm of fluctuation.The optical scanner imaging used time of lesion sample tissue is controlled on 10 minutes left sides
It is right.
Preferably, the optical imagery module 12 can also include:
Image storage and display unit 123, for storing and showing that the scanning and imaging unit 122 carries out optical scanner imaging
Handle the functional diagram and optical section figure of obtained lesion sample tissue.
Preferably, the optical imagery module 12 can also include:
Image processing unit 124, functional diagram and optical section figure for being stored to described image storage and display unit 123
Carry out digital processing.
Wherein, the image data that image storage and display unit 123 obtains batch scanning imaging is according to customer parameter
Setting is stored in local computer, and is shown to operation user for intuitive judgment.User can customize setting image and store
Format, naming method, display mode etc..
Image processing unit 124 can do digital processing to the image data that batch scanning imaging obtains, to improve
The contrast and display effect of image.User can make smooth, filtering according to self-demand to image, go the operation such as background.
The data analysis module 13, the functional diagram and optical section figure that the optical imagery module 12 is obtained with it is existing
Illness database data compares and analyzes, and generates diagnostic result report.
Specifically, the data analysis module 13 includes:
Pathogeny analytical unit 131 is obtained for comparing the illness data in existing database and the optical imagery module 12
The functional diagram and optical section figure arrived to determine the illness close with detection lesion sample tissue most probable, and then infers disease
Source and evolutionary process.
Wherein, with when existing database data comparison operation include but is not limited to image characteristics extraction, Image Feature Matching,
Characteristics of image search etc..
Lesion nature analytical unit 132, for determining and analyzing lesion nature.It may determine that the generation of lesion sample tissue
Position, clinical symptoms, biological behaviour etc., and further analyze the range size of operation plan, the diffusion of disorders post surgery or root
Control situation etc..Such as neoplastic lesion, whether including but not limited to understanding tumour is benign, if it is malignant tumour, life
Long spread condition, Infiltrating extrent, depth, whether there is or not transfers, if tumor resection edge tissues etc..
Diagnostic analysis unit 133 provides diagnostic comments for carrying out medical diagnosis on disease analysis.By combining Such analysis knot
Fruit and algorithms library provide tentative diagnosis result.Diagnostic result provides including but not limited to algorithms library optimum results, empirical disease
It manages doctor and analyzes result etc..
Report generation unit 134, for generating and sending diagnosis report.Complete diagnosis report can be formed and be sent, is given
The final scheme performed the operation out.User can be with customized report template, parameter setting, naming method etc..Report content includes pathogeny
Analysis, lesion nature analysis, diagnostic result etc..
The present embodiment is fixed lesion sample tissue by sample preparation module, light is transparent, fluorescent staining processing
Afterwards, then by optical imagery module to lesion sample tissue prepared by sample preparation module optical imaging scanners are carried out, can obtained
To the functional diagram and optical section figure of lesion sample tissue.It is this to be combined by introducing light transparent technology with optical image technology
Mode, the optical section function of similar actual slice may be implemented, further avoid the actual slice occupied time.And
And since there is no actual slice, lesion sample tissue form and structure save complete, and optical imaging effect clarity can be with
The effect of routine paraffin wax slice pathologic finding compares favourably, and provides accurate diagnostic data for doctor.In addition, with current application
Pathologic finding-quick frozen-section pathologic finding is compared in most arts, a kind of early diagnosis diagnosis provided by the invention
60 minutes quick diagnosis theoretically most may be implemented in system fastly.
Embodiment two
Fig. 2 shows the implementation processes of the method for early diagnosis provided by Embodiment 2 of the present invention diagnosis, are described in detail such as
Under:
In step s 201, lesion sample tissue is extracted from patient's body.
In the embodiment of the present invention, can from patient's body extract lesion sample tissue, for it is subsequent to lesion sample tissue at
Reason provides must material.The operation of extraction includes but is not limited to cut, clamp and take, pierce through, removing.
In step S202, using the fixed lesion sample tissue of chemical reagent.
In the embodiment of the present invention, fixed lesion sample tissue refers to the component in quick solidification lesion sample tissue,
And keep structure when its living body that can be conducive to subsequent transparent to the light of lesion sample tissue after fixing lesion sample tissue
Processing is handled with fluorescent staining.The chemical reagent of fixed lesion sample tissue includes but is not limited to formalin, formaldehyde, and phosphoric acid is slow
Fliud flushing etc. controls at 10 minutes or so the fixation used time of lesion sample tissue.
In step S203, light transparent processing is carried out to the fixed lesion sample tissue using light clarifier.
In the embodiment of the present invention, light transparent processing refers to that the refractive index changed in lesion sample tissue mismatches, and increases light
Penetration power in lesion sample tissue after carrying out light transparent processing to lesion sample tissue, can be convenient subsequent optical imaging
Module carries out the operation of profound optical section to lesion sample tissue.Hereafter lesion sample tissue no longer needs to carry out similar production
Complex operations when paraffin section greatly reduce the time of sample production.Different lesion sample tissues can be directed to
Property using different formulations, the clarifier of different ratio, such as the clarifier in number of patent application CN104568553A, according to lesion sample
The case where this tissue, can suitably change the ratio of fructose, polyethylene glycol, urea, glycerol etc..It is transparent to the light of lesion sample tissue
Used time control is handled at 10 minutes or so.
In step S204, fluorescent staining processing is carried out to the lesion sample tissue that light transparent processing obtains is carried out.
In the embodiment of the present invention, the fluorescent staining of lesion sample tissue is handled, can be convenient subsequent optical image-forming module
The operation of optical scanner imaging is carried out to lesion sample tissue.The selection when selection of fluorescent dye can be imaged according to subsequent optical
Micro imaging system depending on, staining method includes but is not limited to fluorescence immunization coloration, fluorescent antibody staining etc..To lesion sample
The fluorescent staining processing used time of tissue controls at 10 minutes or so.
In step S205, the lesion sample tissue that progress fluorescent staining is handled is placed and fixed.
In the embodiment of the present invention, lesion sample tissue can be anchored on the objective table of functional form micro imaging system,
During ensuring subsequent optical imaging, lesion sample tissue will not be moved.Wherein, the firm mode of sample include but
It is not limited to screw locking, spring leaf press etc..
In step S206, using functional form micro imaging system to carrying out the lesion sample group that handles of fluorescent staining
It knits and carries out optical scanner imaging, obtain the functional diagram and optical section figure of lesion sample tissue.
In the embodiment of the present invention, the lateral two-dimensional scanning of lesion sample tissue is obtained by functional form micro imaging system
The functional diagram of lesion sample tissue；Lesion sample is obtained by longitudinal scanning of the functional form micro imaging system to lesion sample tissue
The optical section figure of this tissue.
It preferably, can be with after the functional diagram and optical section figure for obtaining lesion sample tissue: storing and show and sweep
The functional diagram and optical section figure for the lesion sample tissue retouched, and the functional diagram and optical section figure are carried out at number
Reason.
Specifically, the image data that batch scanning imaging obtains can be stored in local according to customer parameter setting
In computer, and operation user is shown to for intuitive judgment.User can customize format, the name side that setting image stores
Formula, display mode etc..
The image data obtained to batch scanning imaging does digital processing, and the contrast and display of image can be improved
Effect.User can make smooth, filtering according to self-demand to image, go the operation such as background.
In step S207, functional diagram and optical section figure and existing illness database number that scanning imagery is handled
According to comparing and analyzing, diagnostic result report is generated.
In the embodiment of the present invention, the function that illness data and optical imagery module in existing database obtain can be first compared
Can figure and optical section figure, with determine with the close illness of detection lesion sample tissue most probable, and then infer disease source with
Evolutionary process.
Wherein, with when existing database data comparison operation include but is not limited to image characteristics extraction, Image Feature Matching,
Characteristics of image search etc..
It determines again and analysis lesion nature.
Specifically, may determine that the happening part of lesion sample tissue, clinical symptoms, biological behaviour etc., and further
Analyze the range size of operation plan, the diffusion of disorders post surgery or radical cure situation etc..Such as neoplastic lesion, including but not
It is limited to understand whether tumour is benign, and if it is malignant tumour, growth spread condition, Infiltrating extrent, depth, whether there is or not transfers, are
It is no to want tumor resection edge tissues etc..
Then medical diagnosis on disease analysis is carried out, diagnostic comments are provided.
Specifically, by combining Such analysis result and algorithms library to provide tentative diagnosis result.Diagnostic result provides packet
Include but be not limited to algorithms library optimum results, empirical Pathology Doctors ' analysis result etc..
It ultimately produces and sends diagnosis report.
Specifically, complete diagnosis report can be formed and be sent, the final scheme of operation is provided.User customized can report
Slide former, parameter setting, naming method etc..Report content includes pathogeny analysis, lesion nature analysis, diagnostic result etc..
It should be understood that in embodiments of the present invention, magnitude of the sequence numbers of the above procedures are not meant to the elder generation of execution sequence
Afterwards, the execution sequence of each process should be determined by its function and internal logic, the implementation process structure without coping with the embodiment of the present invention
At any restriction.
Those of ordinary skill in the art will appreciate that all or part of the steps in realization the various embodiments described above method is can
It is completed with instructing relevant hardware by program, corresponding program can store in a computer-readable storage medium
In, the storage medium, such as ROM/RAM, disk or CD.
Those of ordinary skill in the art may be aware that list described in conjunction with the examples disclosed in the embodiments of the present disclosure
Member and algorithm steps can be realized with the combination of electronic hardware or computer software and electronic hardware.These functions are actually
It is implemented in hardware or software, the specific application and design constraint depending on technical solution.Professional technician
Each specific application can be used different methods to achieve the described function, but this realization is it is not considered that exceed
The scope of the present invention.
It is apparent to those skilled in the art that for convenience and simplicity of description, the system of foregoing description,
The specific work process of device and unit, can refer to corresponding processes in the foregoing method embodiment, and details are not described herein.
In several embodiments provided herein, it should be understood that disclosed systems, devices and methods, it can be with
It realizes by another way.For example, the apparatus embodiments described above are merely exemplary, for example, the unit
It divides, only a kind of logical function partition, there may be another division manner in actual implementation, such as multiple units or components
It can be combined or can be integrated into another system, or some features can be ignored or not executed.Another point, it is shown or
The mutual coupling, direct-coupling or communication connection discussed can be through some interfaces, the indirect coupling of device or unit
It closes or communicates to connect, can be electrical property, mechanical or other forms.
The unit as illustrated by the separation member may or may not be physically separated, aobvious as unit
The component shown may or may not be physical unit, it can and it is in one place, or may be distributed over multiple
In network unit.It can select some or all of unit therein according to the actual needs to realize the mesh of this embodiment scheme
's.
It, can also be in addition, the functional units in various embodiments of the present invention may be integrated into one processing unit
It is that each unit physically exists alone, can also be integrated in one unit with two or more units.
It, can be with if the function is realized in the form of SFU software functional unit and when sold or used as an independent product
It is stored in a computer readable storage medium.Based on this understanding, technical solution of the present invention is substantially in other words
The part of the part that contributes to existing technology or the technical solution can be embodied in the form of software products, the meter
Calculation machine software product is stored in a storage medium, including some instructions are used so that a computer equipment (can be a
People's computer, server or network equipment etc.) it performs all or part of the steps of the method described in the various embodiments of the present invention.
And storage medium above-mentioned includes: that USB flash disk, mobile hard disk, read-only memory (ROM, Read-Only Memory), arbitrary access are deposited
The various media that can store program code such as reservoir (RAM, Random Access Memory), magnetic or disk.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (5)
1. a kind of system of early diagnosis diagnosis, which is characterized in that the system comprises: sample preparation module, optics at
As module and data analysis module；
The sample preparation module includes:
Tissue collecting's unit, for extracting lesion sample tissue from patient's body；
Fixed cell is organized, the lesion sample tissue for extracting using the fixed tissue collecting's unit of chemical reagent, to institute
The fixation used time for stating lesion sample tissue controlled at 10 minutes, and the chemical reagent of the fixed lesion sample tissue includes formal
Woods, formaldehyde and phosphate buffer；
Light transparent cell is organized, for carrying out light using the light clarifier lesion sample tissue fixed to the tissue fixed cell
Transparent processing, also, for different lesion sample tissues using the clarifier of different formulations and different ratio, the light is transparent
Used time control is handled at 10 minutes；
Tissue staining unit is carried out for carrying out the lesion sample tissue that light transparent processing obtains to the tissue light transparent cell
Fluorescent staining processing, the fluorescent staining processing used time control was at 10 minutes；
The optical imagery module includes:
Sample placement unit carries out the lesion sample that fluorescent staining is handled for placing and fixing the tissue staining unit
Tissue；
Scanning and imaging unit, for carrying out fluorescent staining processing to the tissue staining unit using functional form micro imaging system
Obtained lesion sample tissue carries out optical scanner imaging, and the optical scanner imaging used time controlled at 10 minutes,
Obtain the functional diagram and optical section figure of lesion sample tissue；
The data analysis module, the functional diagram that the scanning and imaging unit is obtained and optical section figure and existing illness data
Library data compare and analyze, and generate diagnostic result report.
2. the system as claimed in claim 1, which is characterized in that the tissue light transparent cell is solid to the tissue fixed cell
Fixed lesion sample tissue carries out light transparent processing specifically: the refractive index changed in lesion sample tissue mismatches, and increases light
Penetration power in lesion sample tissue.
3. the system as claimed in claim 1, which is characterized in that the scanning and imaging unit includes:
First imaging subelement, for being obtained by functional form micro imaging system to the lateral two-dimensional scanning of lesion sample tissue
The functional diagram of lesion sample tissue；
Second imaging subelement, for obtaining lesion by longitudinal scanning of the functional form micro imaging system to lesion sample tissue
The optical section figure of sample tissue.
4. system as claimed in claim 3, which is characterized in that the scanning and imaging unit further include:
Image storage and display unit, for storing and showing that the first imaging subelement and the second imaging subelement scan to obtain
Lesion sample tissue functional diagram and optical section figure；
Image processing unit, functional diagram and optical section figure for storing to described image storage and display unit carry out at number
Reason.
5. the system as claimed in claim 1, which is characterized in that the data analysis module includes:
Pathogeny analytical unit, the function obtained for comparing the illness data in existing database and the optical imagery module scans
It can scheme and optical section figure, deduction disease source and evolutionary process；
Lesion nature analytical unit, for determining and analyzing lesion nature；
Diagnostic analysis unit provides diagnostic comments for carrying out medical diagnosis on disease analysis；
Report generation unit, for generating and sending diagnosis report.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN106548038A (en) * | 2016-12-09 | 2017-03-29 | 江西中科九峰智慧医疗科技有限公司 | A kind of Knowledge based engineering medical imaging acquisition analysis system |
| KR102542371B1 (en) * | 2017-08-22 | 2023-06-13 | 라이카 바이오시스템즈 멜버른 피티와이 엘티디 | Systems and methods for monitoring tissue samples to be processed by a tissue processor |
| CN109979588A (en) * | 2019-01-22 | 2019-07-05 | 北京金智拓科技有限公司 | Image scanning pathological section system |
| CN110398503A (en) * | 2019-02-27 | 2019-11-01 | 广西壮族自治区农业科学院 | A kind of plant pest method of inspection based on geometric shape transmission measurement |
| CN111795879A (en) * | 2020-07-14 | 2020-10-20 | 青岛大学附属医院 | Application of pathological specimen manufactured by dehydration set in digital evaluation system |
| CN112529907B (en) * | 2020-10-19 | 2024-06-14 | 江苏珂玛麒生物科技有限公司 | Rat lung fiber analysis system based on SR dyeing and working method thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0994388A1 (en) * | 1998-10-14 | 2000-04-19 | Eastman Kodak Company | Medical diagnostic film for soft tissue imaging (II) |
| US6102928A (en) * | 1990-03-02 | 2000-08-15 | General Surgical Innovations, Inc. | Method of dissecting tissue layers |
| US6353753B1 (en) * | 1998-05-05 | 2002-03-05 | Stephen Thomas Flock | Optical imaging of deep anatomic structures |
| US7194118B1 (en) * | 2000-11-10 | 2007-03-20 | Lucid, Inc. | System for optically sectioning and mapping surgically excised tissue |
| CN101788709A (en) * | 2010-03-10 | 2010-07-28 | 广西大学 | Digital confocal microscope optical section collecting device |
| CN101487775B (en) * | 2009-01-20 | 2010-12-29 | 天津大学 | Use of borneol preparation in tissue optical clearing agent preparation |
| CN102284066A (en) * | 2011-05-09 | 2011-12-21 | 南开大学 | Novel application of whiteruss as optical transparency reinforcing agent |
| CN102301269A (en) * | 2008-12-05 | 2011-12-28 | 优尼森索股份公司 | Optical Sectioning Of A Sample And Detection Of Particles In A Sample |
| CN102749231A (en) * | 2011-10-14 | 2012-10-24 | 华中科技大学 | Optical clearing agent for bone tissue |
| CN104568553A (en) * | 2014-12-30 | 2015-04-29 | 深圳先进技术研究院 | Tissue optical clearing agent and application thereof |
-
2015
- 2015-12-22 CN CN201510967267.2A patent/CN105606573B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6102928A (en) * | 1990-03-02 | 2000-08-15 | General Surgical Innovations, Inc. | Method of dissecting tissue layers |
| US6353753B1 (en) * | 1998-05-05 | 2002-03-05 | Stephen Thomas Flock | Optical imaging of deep anatomic structures |
| EP0994388A1 (en) * | 1998-10-14 | 2000-04-19 | Eastman Kodak Company | Medical diagnostic film for soft tissue imaging (II) |
| US7194118B1 (en) * | 2000-11-10 | 2007-03-20 | Lucid, Inc. | System for optically sectioning and mapping surgically excised tissue |
| CN102301269A (en) * | 2008-12-05 | 2011-12-28 | 优尼森索股份公司 | Optical Sectioning Of A Sample And Detection Of Particles In A Sample |
| CN101487775B (en) * | 2009-01-20 | 2010-12-29 | 天津大学 | Use of borneol preparation in tissue optical clearing agent preparation |
| CN101788709A (en) * | 2010-03-10 | 2010-07-28 | 广西大学 | Digital confocal microscope optical section collecting device |
| CN102284066A (en) * | 2011-05-09 | 2011-12-21 | 南开大学 | Novel application of whiteruss as optical transparency reinforcing agent |
| CN102749231A (en) * | 2011-10-14 | 2012-10-24 | 华中科技大学 | Optical clearing agent for bone tissue |
| CN104568553A (en) * | 2014-12-30 | 2015-04-29 | 深圳先进技术研究院 | Tissue optical clearing agent and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 光透明剂对生物组织二次谐波成像的影响;陈煌林;《中国优秀硕士学位论文全文数据库 基础科学辑》;20111215(第S1期);第10、18~20页 |
| 现代医学成像技术新进展;陈丹 等;《滨州医学院学报》;20010430;第24卷(第4期);第333~335页 |
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