CN105586383A - Method for rapidly identifying or screening microorganisms producing bacteriostatic active substances - Google Patents
Method for rapidly identifying or screening microorganisms producing bacteriostatic active substances Download PDFInfo
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- CN105586383A CN105586383A CN201511001121.9A CN201511001121A CN105586383A CN 105586383 A CN105586383 A CN 105586383A CN 201511001121 A CN201511001121 A CN 201511001121A CN 105586383 A CN105586383 A CN 105586383A
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- microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
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- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for rapidly identifying or screening microorganisms producing bacteriostatic active substances. The method comprises steps as follows: a solid culture medium culture dish (an LB culture medium or a PDA culture medium and the like) is prepared; tested microorganisms and indicator microorganisms are inoculated to the same culture dish under the sterile condition with a cross-streak method; after inoculation, the culture dish is put in a culture box for inverted culture under the condition of 26-40 DEG C for 12-240 h; whether the tested microorganisms produce the active substances for inhibiting the microorganisms is determined. By means of the method, whether the tested microorganisms produce the active substances for inhibiting the indicator microorganisms can be rapidly identified or the microorganisms producing the active substances for inhibiting the indicator microorganisms can be rapidly screened, the steps are simple, and the efficiency is high.
Description
Technical field
The present invention relates to Experiment on Microbiology technical field, relate in particular to a kind of Rapid identification and produce SubstanceMicroorganism or screening produce indicator microoraganism had to the method for microorganism of inhibiting active material.
Background technology
At present, identifying or screen a kind of microorganism, whether to produce the method for antibacterial or Substance mainBe agar diffusion method, disk diffusion method, drug sensitive test (being inhibition zone method), growth rate method, face-off method,Cylinder plate method etc.
In prior art, be all often first to operate respectively, first obtain for trying the tunning of microorganism or having thisThe agar block of microorganism, re-uses corresponding methods experiment, if can suppress indicator bacteria for examination microorganismsThe active material of growth, thus the phenomenons such as transparent circle produced, determine accordingly the pressing down indicator microoraganism for examination bacteriumFunction processed. But so need need operate respectively for examination microorganism and indicator microoraganism, first obtain for the micro-life of examinationThe tunning of thing or have the agar block of this microorganism, re-uses corresponding methods experiment, if for the micro-life of examinationDeposits yields can suppress the active material of indicator bacteria growth, thereby produces the phenomenons such as transparent circle, determines accordingly for examinationThe inhibit feature of bacterium to indicator microoraganism. Prior art complex steps, efficiency is low.
Summary of the invention
The object of the invention is Rapid identification for examination microorganism whether produce indicator microoraganism is had inhibitingThe microorganism that indicator microoraganism is had to inhibiting active material is produced in active material or screening.
The method of microorganism of Substance is produced in a kind of Rapid identification that the present invention proposes or screening, comprisesFollowing steps:
S1, prepare solid medium culture dish (LB culture medium or PDA culture medium etc.);
The streak inoculation that intersects on same culture dish under S2, aseptic condition is for examination microorganism and indicator microoraganism;
After S3, inoculation, culture dish is placed in incubator and cultivates (condition of culture: 26-40 DEG C, incubation time 12-240h)；
S4, definite active material that whether produces inhibition indicator microoraganism for examination microorganism.
The present invention has simplified traditional qualification and whether has produced the active material step that suppresses indicator bacteria for examination microorganismSuddenly, shorten the experiment used time, can multiplely identify for examination microorganism simultaneously, improved efficiency, saved screeningCost.
Brief description of the drawings
Fig. 1 is the schematic diagram of the embodiment of the present invention 1;
Fig. 2 is the schematic diagram of the embodiment of the present invention 2;
Fig. 3 is the result photo of the embodiment of the present invention 3.
Detailed description of the invention
Below, by specific embodiment, technical scheme of the present invention is elaborated.
Embodiment 1
The method of microorganism of Substance is produced in a kind of Rapid identification that the present invention proposes or screening, comprisesFollowing steps:
S1, prepare solid medium culture dish (LB culture medium or PDA culture medium etc.);
The streak inoculation that intersects on same culture dish under S2, aseptic condition is for examination microorganism and indicator microoraganism;
After S3, inoculation, culture dish is placed in incubator and cultivates (condition of culture: 26-40 DEG C, incubation time 12-240h)；
S4, definite active material that whether produces inhibition indicator microoraganism for examination microorganism.
As shown in Figure 1, Fig. 1 is the schematic diagram of the embodiment of the present invention 1. As shown in Figure 1 for examination microorganism ADo not produce the active material that suppresses indicator microoraganism O, supply examination microorganism A and indicator microoraganism at infallO coexists.
Embodiment 2
The method of microorganism of Substance is produced in a kind of Rapid identification that the present invention proposes or screening, comprisesFollowing steps:
S1, prepare solid medium culture dish (LB culture medium or PDA culture medium etc.);
The streak inoculation that intersects on same culture dish under S2, aseptic condition is for examination microorganism and indicator microoraganism;
After S3, inoculation, culture dish is placed in incubator and cultivates (condition of culture: 26-40 DEG C, incubation time 12-240h)；
S4, definite active material that whether produces inhibition indicator microoraganism for examination microorganism.
As shown in Figure 2, Fig. 2 is the schematic diagram of the embodiment of the present invention 2. As shown in Figure 2 for examination microorganism BProduce the active material that suppresses indicator microoraganism O, only had the growth for examination microorganism B at infall, and refer toShow that microorganism O is suppressed.
Embodiment 3
The method of microorganism of Substance is produced in a kind of Rapid identification that the present invention proposes or screening, comprisesFollowing steps:
S1, prepare solid medium culture dish (LB culture medium or PDA culture medium etc.);
The streak inoculation that intersects on same culture dish under S2, aseptic condition is for examination Microbial biomass C (BacillusAnd indicator microoraganism D (Escherichiacoli) amyloliquefaciens);
S3, by the culture dish after microbe inoculation be placed in incubator cultivate (condition of culture: 26-40 DEG C, trainingSupport time 12-240h);
S4, definite active material that whether produces inhibition indicator microoraganism for examination microorganism.
As shown in Figure 3, Fig. 3 is the result photo of the embodiment of the present invention 3. As shown in Figure 3 for examination microorganismC (Bacillusamyloliquefaciens) has produced the activity of inhibition indicator microoraganism D (Escherichiacoli)Material, only has for examination Microbial biomass C (Bacillusamyloliquefaciens) growth at infall, and indicates micro-lifeThing D (Escherichiacoli) is suppressed.
The above, be only preferably detailed description of the invention of the present invention, but not office of protection scope of the present inventionBe limited to this, any be familiar with those skilled in the art the present invention disclose technical scope in, according to thisThe technical scheme of invention and inventive concept thereof are equal to replaces or changes, and all should be encompassed in protection of the present inventionWithin scope.
Claims (1)
1. a method of microorganism for Substance is produced in Rapid identification or screening, it is characterized in that bagDraw together following steps:
S1, prepare solid medium culture dish (LB culture medium or PDA culture medium etc.);
The streak inoculation that intersects on same culture dish under S2, aseptic condition is for examination microorganism and indicator microoraganism;
After S3, inoculation, culture dish is placed in incubator and cultivates (condition of culture: 26-40 DEG C, incubation time 12-240h)；
S4, determine for examination microorganism whether produce suppress indicator microoraganism active material or screening produce to instructionMicroorganism has the microorganism of inhibiting active material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511001121.9A CN105586383A (en) | 2015-12-28 | 2015-12-28 | Method for rapidly identifying or screening microorganisms producing bacteriostatic active substances |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511001121.9A CN105586383A (en) | 2015-12-28 | 2015-12-28 | Method for rapidly identifying or screening microorganisms producing bacteriostatic active substances |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105586383A true CN105586383A (en) | 2016-05-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201511001121.9A Pending CN105586383A (en) | 2015-12-28 | 2015-12-28 | Method for rapidly identifying or screening microorganisms producing bacteriostatic active substances |
Country Status (1)
| Country | Link |
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| CN (1) | CN105586383A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4946777A (en) * | 1973-05-31 | 1990-08-07 | Gist-Brocades N.V. | Method for determination of the presence of antibiotics |
| CN103667149A (en) * | 2013-12-12 | 2014-03-26 | 台州职业技术学院 | Strain with antibacterial activity as well as screening method and application thereof |
-
2015
- 2015-12-28 CN CN201511001121.9A patent/CN105586383A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4946777A (en) * | 1973-05-31 | 1990-08-07 | Gist-Brocades N.V. | Method for determination of the presence of antibiotics |
| CN103667149A (en) * | 2013-12-12 | 2014-03-26 | 台州职业技术学院 | Strain with antibacterial activity as well as screening method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| RAYK A.SALOMON ET AL: "The Peptide Antibiotic Microcin 25 Is Imported through the TonB Pathway and the SbmA Protein", 《JOURNAL OF BACTERIOLOGY》 * |
| 肖建根: "PB6活性菌株对产气荚膜梭菌和弧形杆菌的抑制能力的研究", 《中国畜牧杂志》 * |
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Application publication date: 20160518 |