CN105586332A - Method for immobilized microorganisms by utilization of porous ore - Google Patents
Method for immobilized microorganisms by utilization of porous ore Download PDFInfo
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- CN105586332A CN105586332A CN201610136236.7A CN201610136236A CN105586332A CN 105586332 A CN105586332 A CN 105586332A CN 201610136236 A CN201610136236 A CN 201610136236A CN 105586332 A CN105586332 A CN 105586332A
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- ore deposit
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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Abstract
The invention discloses a method for immobilized microorganisms by the utilization of porous ore. The method comprises the following steps that the porous ore is subjected to activation treatment; a prepared microorganism bacterium agent is added into the activated porous ore; then a cross-lining agent and a binding agent are added and stirred and mixed uniformly; a porous ore carrier matrix containing the microorganism bacterium agent is subjected to palletizing treatment to be prepared into an immobilized microorganism carrier in a certain shape. The prepared microorganism immobilized carrier is high in mechanical strength and good in permeability, the immobilized microorganisms do not fall off easily, and the service life is long.
Description
Technical field
The present invention relates to sewage purification technology field, particularly one is utilized porous ore deposit process for immobilizing enzyme/microbe.
Background technology
Porous ore deposit there is a large amount of holes and duct, strong ion exchangeable and compared with strong adsorption capacity, specific area is large, physicochemical properties are stable,, Resistance to microbes nontoxic to microorganism, can not be to discharging the advantages such as poisonous and harmful substance, price be lower in water. Such as medical stone adsorption rate is 20000 times of equal surface area activated carbon, colibacillary adsorption rate is reached more than 96%.
Microbial immobilized technology refers to utilizes chemistry or physical means that free microorganism is fixed in a certain local space region, keeps the activity of microorganism simultaneously, and a biotechnology that it can be repeated and use continuously. Microbial immobilized method comprises: absorption method, covalent coupling method, cross-linking method and investment. Immobilized microorganism has that microbe density is high, treatment effeciency is high, resistance to murder by poisoning and capacity of resisting impact load is strong, stability strong, can purifying and keep the advantages such as efficient dominant bacteria. Aspect sewage disposal, studied widely and applied. The present invention uses porous ore deposit as Immohilized microorganism carrier, the compound process for fixation immobilized microorganism combining by the absorption of porous ore deposit, glutaraldehyde cross-linking, adhesive embedding.
Summary of the invention
The shortcoming existing for solving prior art, the invention provides a kind of porous ore deposit process for immobilizing enzyme/microbe that utilizes.
One is utilized porous ore deposit process for immobilizing enzyme/microbe, it is characterized in that, it comprises the following steps:
The activation processing in porous ore deposit: porous ore deposit is pulverized, and with the salt acid soak 1-3 hour of 5%-15%, after cleaning up by purified water, high-temperature calcination 3-12 hour, is cooled to normal temperature;
The preparation of microbial inoculum: the microorganism of centrifugal collection is added to SPSS, clean after 2-3 time, making concentration with SPSS is 2 × 1011-9×1011Cfu/mL bacterium liquid;
Immobilization: microbial inoculum is added in the porous ore deposit after activation processing, stir and evenly mix, adsorb 30 minutes, then adding concentration is 1.5% crosslinking agent glutaraldehyde solution, after stirring and evenly mixing, add again adhesive, do not add a certain amount of water, stir and evenly mix, granulation, makes the effigurate fixed microorganism carrier of tool.
Described porous ore deposit is any one or combination of medical stone, zeolite, volcanic rock, diatomite, attapulgite, montmorillonite.
The broken granularity of described porous breeze is 100-200 order.
Described calcining heat is 200-400 degree Celsius.
The addition of described crosslinking agent glutaraldehyde solution is the 5%-35% of porous ore deposit weight.
Described adhesive is a kind of or combination of polyvinyl alcohol, polyethylene glycol, polyacrylamide, sodium alginate; The addition of described adhesive is the 5%-20% of porous ore deposit weight.
Technical scheme of the present invention, in preparation process, taking porous ore deposit as carrier, by compound process for fixation, microorganism is combined with carrier firmly, difficult drop-off, mechanical strength is high, and permeability is good, long service life. The method technique is simple, is applicable to suitability for industrialized production. When immobilized microorganism purifies for aquarium water quality, can also make the shape of certain ornamental value, there is purification of water quality and ornamental value dual-use function.
Brief description of the drawings
Fig. 1 utilizes the immobilized nitrobacteria figure of the present invention.
Detailed description of the invention
Below in conjunction with example, the present invention is done to further concrete description, but enforcement of the present invention is not limited to this.
Embodiment 1
Medical stone powder is broken to 100 orders, adds 5% hydrochloric acid solution to invade bubble 3 hours, after cleaning up by purified water, 300 celsius temperatures calcinings 4 hours, are cooled to normal temperature, for subsequent use. The nitrobacteria of centrifugal collection is added to SPSS, clean after 3 times, making concentration with SPSS is 5 × 1011Cfu/mL bacterium liquid, gets 2ml bacterium liquid and adds in the medical stone after 100g activation, stirs and evenly mixs, and adsorbs 30 minutes, then add the glutaraldehyde solution of 8ml, after mixing, add 10g polyvinyl alcohol, mix, add a certain amount of purified water, mix granulation, obtain the fixed microorganism carrier of definite shape.
Embodiment 2
Diatomite in powder is broken to 150 orders, adds 8% hydrochloric acid solution to invade bubble 2 hours, after cleaning up by purified water, 200 DEG C of high-temperature calcinations 6 hours, are cooled to normal temperature, for subsequent use. The photosynthetic bacteria of centrifugal collection is added to SPSS, clean after 2 times, making concentration with SPSS is 4 × 1011Cfu/mL bacterium liquid, gets 3ml bacterium liquid and adds in the medical stone after 100g activation, stirs and evenly mixs, and adsorbs 30 minutes, then add the glutaraldehyde solution of 6ml, after mixing, add 8g polyethylene glycol, mix, add a certain amount of purified water, mix granulation, obtain the fixed microorganism carrier of definite shape.
Embodiment 3
Volcanic rock is crushed to 200 orders, adds 10% hydrochloric acid solution to invade bubble 1 hour, after cleaning up by purified water, 400 DEG C of high-temperature calcinations 3 hours, are cooled to normal temperature, for subsequent use. The photosynthetic bacteria of centrifugal collection is added to SPSS, clean after 3 times, making concentration with SPSS is 8 × 1011Cfu/mL bacterium liquid, gets 5ml bacterium liquid and adds in the medical stone after 100g activation, stirs and evenly mixs, and adsorbs 30 minutes, then add the glutaraldehyde solution of 10ml, after mixing, add 12g polyethylene glycol, mix, add a certain amount of purified water, mix granulation, obtain the fixed microorganism carrier of definite shape.
Claims (6)
1. utilize a porous ore deposit process for immobilizing enzyme/microbe, it is characterized in that, it comprises the following steps:
The activation processing in porous ore deposit: porous ore deposit is pulverized, and with the salt acid soak 1-3 hour of 5%-15%, after cleaning up by purified water, high-temperature calcination 3-12 hour, is cooled to normal temperature;
The preparation of microbial inoculum: the microorganism of centrifugal collection is added to SPSS, clean after 2-3 time, making concentration with SPSS is 2 × 1011-9×1011Cfu/mL bacterium liquid;
Immobilization: microbial inoculum is added in the porous ore deposit after activation processing, stir and evenly mix, adsorb 30 minutes, then adding concentration is 1.5% crosslinking agent glutaraldehyde solution, after stirring and evenly mixing, add again adhesive, do not add a certain amount of water, stir and evenly mix, granulation, makes the effigurate fixed microorganism carrier of tool.
2. a kind of method of utilizing porous ore deposit fixation of microbe according to claim 1, is characterized in that: described porous ore deposit is any one or combination of medical stone, zeolite, volcanic rock, diatomite, attapulgite, montmorillonite.
3. a kind of method of utilizing porous ore deposit fixation of microbe according to claim 1, is characterized in that: the broken granularity of described porous breeze is 100-200 order.
4. a kind of method of utilizing porous ore deposit fixation of microbe according to claim 1, is characterized in that: described calcining heat is 200-400 degree Celsius.
5. a kind of method of utilizing porous ore deposit fixation of microbe according to claim 1, is characterized in that: the addition of described crosslinking agent glutaraldehyde solution is the 5%-35% of porous ore deposit weight.
6. a kind of method of utilizing porous ore deposit fixation of microbe according to claim 1, is characterized in that: described adhesive is a kind of or combination of polyvinyl alcohol, polyethylene glycol, polyacrylamide, sodium alginate; The addition of described adhesive is the 5%-20% of porous ore deposit weight.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610136236.7A CN105586332A (en) | 2016-03-11 | 2016-03-11 | Method for immobilized microorganisms by utilization of porous ore |
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| CN201610136236.7A CN105586332A (en) | 2016-03-11 | 2016-03-11 | Method for immobilized microorganisms by utilization of porous ore |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106993720A (en) * | 2017-05-18 | 2017-08-01 | 颍上县博陵家庭农场 | A kind of mixed feed for improving black pig appetite |
| CN107183377A (en) * | 2017-05-18 | 2017-09-22 | 颍上县博陵家庭农场 | A kind of black pig feed for promoting to grow |
| CN107674869A (en) * | 2017-11-06 | 2018-02-09 | 中国科学院寒区旱区环境与工程研究所 | A kind of preparation, measure and the experimental method of attapulgite immobilization oil degradation microbial inoculum |
| CN111333200A (en) * | 2020-03-18 | 2020-06-26 | 运城学院 | Embedded immobilized microorganism particles, preparation method and sewage treatment method |
| CN111534507A (en) * | 2020-06-17 | 2020-08-14 | 武汉工程大学 | Immobilized microbial agent for raw ore of clay mineral, preparation method and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1982447A (en) * | 2005-12-16 | 2007-06-20 | 中国科学院沈阳应用生态研究所 | Fungus and bacterium combined curing method with roseite as carrier |
| CN101319212A (en) * | 2008-07-18 | 2008-12-10 | 北京化工大学 | Immobilization method for anaerobic fermentation bacterial active sludge |
| CN101701216A (en) * | 2009-11-27 | 2010-05-05 | 安徽农业大学 | Preparation method of microbe immobilized particles for restoring pesticide contaminated soil |
| CN102653754A (en) * | 2012-05-29 | 2012-09-05 | 中华人民共和国沈阳出入境检验检疫局 | Preparation method of microorganism immobilization particles for removing residual pesticide in soil |
-
2016
- 2016-03-11 CN CN201610136236.7A patent/CN105586332A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1982447A (en) * | 2005-12-16 | 2007-06-20 | 中国科学院沈阳应用生态研究所 | Fungus and bacterium combined curing method with roseite as carrier |
| CN101319212A (en) * | 2008-07-18 | 2008-12-10 | 北京化工大学 | Immobilization method for anaerobic fermentation bacterial active sludge |
| CN101701216A (en) * | 2009-11-27 | 2010-05-05 | 安徽农业大学 | Preparation method of microbe immobilized particles for restoring pesticide contaminated soil |
| CN102653754A (en) * | 2012-05-29 | 2012-09-05 | 中华人民共和国沈阳出入境检验检疫局 | Preparation method of microorganism immobilization particles for removing residual pesticide in soil |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106993720A (en) * | 2017-05-18 | 2017-08-01 | 颍上县博陵家庭农场 | A kind of mixed feed for improving black pig appetite |
| CN107183377A (en) * | 2017-05-18 | 2017-09-22 | 颍上县博陵家庭农场 | A kind of black pig feed for promoting to grow |
| CN107674869A (en) * | 2017-11-06 | 2018-02-09 | 中国科学院寒区旱区环境与工程研究所 | A kind of preparation, measure and the experimental method of attapulgite immobilization oil degradation microbial inoculum |
| CN111333200A (en) * | 2020-03-18 | 2020-06-26 | 运城学院 | Embedded immobilized microorganism particles, preparation method and sewage treatment method |
| CN111534507A (en) * | 2020-06-17 | 2020-08-14 | 武汉工程大学 | Immobilized microbial agent for raw ore of clay mineral, preparation method and application |
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