CN105572387A - Kit for detecting nuclear matrix protein 22 through immunofluorescence chromatography and preparation method of kit - Google Patents
Kit for detecting nuclear matrix protein 22 through immunofluorescence chromatography and preparation method of kit Download PDFInfo
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- CN105572387A CN105572387A CN201610053411.6A CN201610053411A CN105572387A CN 105572387 A CN105572387 A CN 105572387A CN 201610053411 A CN201610053411 A CN 201610053411A CN 105572387 A CN105572387 A CN 105572387A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6875—Nucleoproteins
Abstract
The invention relates to the technical field of medical supplies, and particularly discloses a kit for detecting nuclear matrix protein 22 through immunofluorescence chromatography and a preparation method of the kit. The kit comprises a buffer solution and a reagent card. The buffer solution is a phosphate buffer solution containing fluorescently-labeled rabbit anti-human nuclear matrix protein 22 antibodies, wherein the concentration of the fluorescently-labeled rabbit anti-human nuclear matrix protein 22 antibodies is 0.25-0.92 nanograms per microliter. The reagent card comprises a plastic cushion plate. A nitrocellulose membrane is arranged on the plastic cushion plate. A detection line and a quality control line are arranged on the nitrocellulose membrane in a spaced and left-right mode, a glass cellulose membrane is arranged on the portion, on the left side of the detection line, of the nitrocellulose membrane. A piece of water absorption paper is arranged on the portion, on the right side of the quality control line, of the nitrocellulose membrane. A blood filter membrane is arranged on the glass cellulose membrane. The detection line is wrapped by rabbit anti-human nuclear matrix protein 22 antibodies. The quality control line is wrapped by goat anti-rabbit antibodies. The kit has a good linear range and is high in detection flexibility, repeatability and accuracy.
Description
Technical field
The present invention relates to field of medical article technology, particularly relate to biological and medical science detection field of medical article technology, be specifically related to a kind of immunofluorescence chromatography and detect NMP-22 kit and preparation method thereof.
Background technology
Carcinoma of urinary bladder refers to the malignant tumour occurred in bladder mucosa.Be the modal malignant tumour of urinary system, be also one of large kinds of tumor of whole body ten, account for first of China's Genitourinary system incidence of disease, and its incidence of disease be only second to prostate cancer in west, occupies the 2nd.It is 6.61/10 ten thousand that national tumour in 2012 registers the incidence of disease of regional carcinoma of urinary bladder, the 9th of row Cancer Mortality.Carcinoma of urinary bladder can betide any age, even children.Its incidence of disease increases with the age and increases, 50 ~ 70 years old age occurred frequently, and male bladder cancer morbidity is 3 ~ 4 times of women.Current diagnosis carcinoma of urinary bladder mainly Urine exfoliated cells detects and endoscopy, but exfoliative cell examination of urine sensitivity is too low, only has 34%, and endoscopy has wound inspection to be not suitable for health check-up, is only suitable for making a definite diagnosis.
NMP-22 (NMP22) is the one of nuclear matrix protein family, is made up of 2101 amino acid, and NMP22 is the index found in recent years as the biomarker of carcinoma of urinary bladder.Time with 10U/ milliliter for diagnosis reference value, the sensitivity of NMP22 diagnosing bladder cancer reaches 82.76%, when with NMP22 health check-up, positive endoscope is made a definite diagnosis, 86% is reached to the sensitivity of Diagnosis of Bladder, specificity reaches 97%, so NMP22 has unusual scientific value and economic worth as diagnosis marker.
Therefore, research and develop a kind of simple, fast, Accurate Determining goes out the method for the concentration of NMP-22, has very large clinical value and meaning for the early diagnosis of carcinoma of urinary bladder and prevention.
Summary of the invention
The object of this invention is to provide a kind of immuno-fluorescence assay NMP-22 kit, the concentration of the mensuration NMP-22 that this kit can be sensitive, quick, accurate, special.
Meanwhile, the present invention also aims to the preparation method that a kind of immuno-fluorescence assay NMP-22 kit is provided.
In order to realize above object, the present invention adopts following technical scheme:
A kind of immunofluorescence chromatography detects NMP-22 kit, comprises damping fluid and reagent card;
Described damping fluid is the phosphate buffer containing the anti-human NMP-22 antibody of the fluorescently-labeled rabbit of 0.25 ~ 0.92ng/ μ l;
Described reagent card comprises test strips, described test strips comprises plastic base plate, plastic base plate arranges nitrocellulose filter, the upper left right septum of nitrocellulose filter arranges detection line and nature controlling line, on the left of detection line, nitrocellulose filter is arranged glass fibre element film, on the right side of nature controlling line, nitrocellulose filter arranges thieving paper, glass fibre element film arranges hemofiltration film; Described detection line wrap by mouse-anti people NMP-22 antibody; Described nature controlling line wraps by goat anti-rabbit antibody.
Described reagent card also comprises shell, and described shell comprises the upper cover and lower cover that link together, covers and be provided with blood sample hole and detection window on described; The position of the corresponding hemofiltration film in position in described blood sample hole; The corresponding detection line in position of described detection window and the position of nature controlling line, make detection line and nature controlling line be in detection window.
On described detection line, the package amount of mouse-anti people NMP-22 antibody is 50 ~ 160ng/cm.
On described nature controlling line, the package amount of goat anti-rabbit antibody is 0.3 ~ 1.0 μ g/cm.
PH=6.5 ~ 7.3 of described phosphate buffer, phosphate concn is 30 ~ 80mmol/L.
Spacing distance between described detection line and nature controlling line is 5mm ~ 10mm.
The width of described reagent strip is 3mm ~ 6mm.
The excitation wavelength of the fluorescein that the anti-human NMP-22 antibody of described fluorescently-labeled rabbit adopts is 652nm, and wavelength of transmitted light is 668nm.
The operation steps of the using method of described kit comprises:
I: the NMP-22 standard items getting variable concentrations join in damping fluid, join on the hemofiltration film of reagent card after mixing, after room temperature water placing flat 10 ~ 15min, reagent card is inserted fluorescence immunoassay readout instrument, setting excitation wavelength is 652nm, wavelength of transmitted light is 668nm, read the fluorescent value of variable concentrations NMP-22 standard items, and the concentration value of fluorescent value and NMP-22 standard items is carried out linear analysis, draw fluorescent value and NMP-22 concentration value typical curve, obtain the concentration relationship formula of fluorescent value and NMP-22;
II: get testing sample and join in damping fluid, join on the hemofiltration film of reagent card after mixing, room temperature water placing flat 10 ~ 15min, reagent card is inserted fluorescence immunoassay readout instrument, setting excitation wavelength is 652nm, and wavelength of transmitted light is 668nm, reads the fluorescent value of testing sample, this fluorescent value is brought in the relational expression that step I obtains, calculate the concentration of testing sample NMP-22.
Described kit containment, under 4 ~ 8 DEG C of environment, re-uses after first damping fluid at room temperature being balanced 15 minutes in use.
Above-mentioned immunofluorescence chromatography detects the preparation method of NMP-22 kit, comprises following operation steps:
1) damping fluid is prepared: get the anti-human NMP-22 antibody of fluorescently-labeled rabbit and add in phosphate buffer, mix, obtain damping fluid;
2) reagent card is prepared: get nitrocellulose filter, mark detection line and nature controlling line, then wrap by mouse-anti people NMP-22 antibody on detection line, nature controlling line wraps by goat anti-rabbit antibody, then nitrocellulose filter, glass fibre element film, thieving paper, hemofiltration film are overlapped on plastic base plate successively, and cut into the reagent card of required width;
3) by step 1) damping fluid, the step 2 prepared) reagent card prepared, assembling, obtains described immunofluorescence chromatography and detects NMP-22 kit.
Above-mentioned steps 2) in adopt the mode of specking to wrap on detection line by mouse-anti people NMP-22 antibody, nature controlling line wraps by goat anti-rabbit antibody.
Immunofluorescence chromatography of the present invention detects NMP-22 kit, comprise damping fluid and chromatography reagent card, the anti-human nuclear matrix protein of fluorescently-labeled rabbit is added as capture antibody in damping fluid, reagent card detection line wraps by mouse-anti people NMP-22 antibody as detection antibody, employing fluorescence immune chromatography antibody sandwich ratio juris realizes the detection to NMP-22 concentration in urine.It is good that this kit detects the NMP-22 range of linearity, and the range of linearity is 2.5 ~ 187U/ml, linearly dependent coefficient R >=0.990, positive predictive value scope >=10U/ml; Simultaneously kit of the present invention detect NMP-22 reproducible, accuracy is high, coefficient of variation CV≤8% of standard items repeated test acquired results, relative standard deviation≤9%.
Immunofluorescence chromatography of the present invention detects the preparation method of NMP-22 kit, easy and simple to handle, is easy to control, and is suitable for industrial application.
Accompanying drawing explanation
The immunofluorescence chromatography that Fig. 1 provides for the embodiment of the present invention 1 detects the reagent card structural representation in NMP-22 kit;
The immunofluorescence chromatography detection NMP-22 kit enclosure structural representation that Fig. 2 provides for the embodiment of the present invention 1.
Embodiment
Below by specific embodiment, technical scheme of the present invention is described in detail.
In following embodiment and test example, the anti-human NMP-22 antibody of fluorescently-labeled rabbit is purchased from Abcam, and article number is ab204018; Mouse-anti people NMP-22 antibody is purchased from Abcam, and article number is ab5675; NMP-22 antibody standard substance is purchased from Abcam.
Embodiment 1
This enforcement immunofluorescence chromatography detects NMP-22 kit, comprises damping fluid and reagent card;
Wherein damping fluid is pH=7.0 containing the anti-human NMP-22 antibody of the fluorescently-labeled rabbit of 0.5ng/ μ l, phosphate concn is the phosphate buffer of 50mmol/L; Wherein the excitation wavelength of the fluorescein of fluorescence labeling employing is 652nm, and wavelength of transmitted light is 668nm;
Described reagent card comprises test strips, described reagent strip width is 5mm, as shown in Figure 1, comprise plastic base plate 1, plastic base plate is arranged nitrocellulose filter 2, the upper left right septum 7mm of nitrocellulose filter 2 arranges detection line 3 and nature controlling line 4, on the left of detection line 3, nitrocellulose filter 2 is arranged glass fibre element film 5, on the right side of nature controlling line 4, nitrocellulose filter 2 arranges thieving paper 6, glass fibre element film 5 arranges hemofiltration film 7; On described detection line, every centimetre of bag is by 100ng mouse-anti people NMP-22 antibody; On described nature controlling line, every centimetre of bag is by 0.6 μ g goat anti-rabbit antibody;
Described reagent card also comprises shell, and described test strips is positioned in described shell, and as shown in Figure 2, described shell comprises the upper cover and lower cover that link together, covers and be provided with blood sample hole 8 and detection window 9 on described; The position of the corresponding hemofiltration film in position in described blood sample hole 8; The corresponding detection line in position of described detection window 9 and the position of nature controlling line, make detection line and nature controlling line be in detection window; Described outer casing upper cover detection window right side blank place can be used as the record surface 10 for recording data.
The present embodiment immunofluorescence chromatography detects the preparation method of NMP-22 kit, comprises following operation steps:
1) damping fluid is prepared: get fluorescently-labeled rabbit anti-human NMP-22 antibody 30ng, add 60 μ l, pH=7.0, phosphate concn are in the phosphate buffer of 50mmol/L, mix, obtain damping fluid;
2) reagent card is prepared: get nitrocellulose filter, mark detection line and nature controlling line, then on detection line specking bag by mouse-anti people NMP-22 antibody, on nature controlling line, specking bag is by goat anti-rabbit antibody, then nitrocellulose filter, glass fibre element film, thieving paper, hemofiltration film are overlapped on plastic base plate successively, and cut into required width, then load in the shell be joined together to form by upper cover and lower cover, obtain described reagent card; Wherein outer casing upper cover is provided with blood sample hole and detection window, the position of the corresponding hemofiltration film in position in described blood sample hole, the corresponding detection line in position of described detection window and the position of nature controlling line, make detection line and nature controlling line be in detection window, described outer casing upper cover detection window right side blank place can be used as the record surface of record data;
3) by step 1) damping fluid, the step 2 prepared) reagent card prepared, assembling, obtains described immunofluorescence chromatography and detects NMP-22 kit.
The present embodiment kit containment is under 4 ~ 8 DEG C of environment.
Embodiment 2
This enforcement immunofluorescence chromatography detects NMP-22 kit, comprises damping fluid and reagent card;
Wherein damping fluid is pH=6.5 containing the anti-human NMP-22 antibody of the fluorescently-labeled rabbit of 0.25ng/ μ l, phosphate concn is the phosphate buffer of 80mmol/L; Wherein the excitation wavelength of the fluorescein of fluorescence labeling employing is 652nm, and wavelength of transmitted light is 668nm;
Described reagent card comprises test strips, described test strips width is 3mm, comprise plastic base plate, plastic base plate arranges nitrocellulose filter, the upper left right septum 5mm of nitrocellulose filter arranges detection line and nature controlling line, on the left of detection line, nitrocellulose filter is arranged glass fibre element film, on the right side of nature controlling line, nitrocellulose filter arranges thieving paper, glass fibre element film arranges hemofiltration film; On described detection line, every centimetre of bag is by 50ng mouse-anti people NMP-22 antibody; On described nature controlling line, every centimetre of bag is by 0.3 μ g goat anti-rabbit antibody;
Described reagent card also comprises shell, and described test strips is positioned in described shell, and described shell comprises the upper cover and lower cover that link together, covers and be provided with blood sample hole and detection window on described; The position of the corresponding hemofiltration film in position in described blood sample hole; The corresponding detection line in position of described detection window and the position of nature controlling line, make detection line and nature controlling line be in detection window; Described outer casing upper cover detection window right side blank place can be used as the record surface for recording data.
The present embodiment immunofluorescence chromatography detects the preparation method of NMP-22 kit, comprises following operation steps:
1) damping fluid is prepared: get fluorescently-labeled rabbit anti-human NMP-22 antibody 15ng, add 60 μ l, pH=6.5, phosphate concn are in the phosphate buffer of 80mmol/L, mix, obtain damping fluid;
2) reagent card is prepared: get nitrocellulose filter, mark detection line and nature controlling line, then on detection line specking bag by mouse-anti people NMP-22 antibody, on nature controlling line, specking bag is by goat anti-rabbit antibody, then nitrocellulose filter, glass fibre element film, thieving paper, hemofiltration film are overlapped on plastic base plate successively, and cut into required width, then load in the shell be joined together to form by upper cover and lower cover, obtain described reagent card; Wherein outer casing upper cover is provided with blood sample hole and detection window, the position of the corresponding hemofiltration film in position in described blood sample hole, the corresponding detection line in position of described detection window and the position of nature controlling line, make detection line and nature controlling line be in detection window, described outer casing upper cover detection window right side blank place can be used as the record surface of record data;
3) by step 1) damping fluid, the step 2 prepared) reagent card prepared, assembling, obtains described immunofluorescence chromatography and detects NMP-22 kit.
The present embodiment kit containment is under 4 ~ 8 DEG C of environment.
Embodiment 3
This enforcement immunofluorescence chromatography detects NMP-22 kit, comprises damping fluid and reagent card;
Wherein damping fluid is pH=7.0 containing the anti-human NMP-22 antibody of the fluorescently-labeled rabbit of 0.92ng/ μ l, phosphate concn is the phosphate buffer of 50mmol/L; Wherein the excitation wavelength of the fluorescein of fluorescence labeling employing is 652nm, and wavelength of transmitted light is 668nm;
Described reagent card comprises test strips, described test strips width is 6mm, comprise plastic base plate, plastic base plate arranges nitrocellulose filter, the upper left right septum 10mm of nitrocellulose filter arranges detection line and nature controlling line, on the left of detection line, nitrocellulose filter is arranged glass fibre element film, on the right side of nature controlling line, nitrocellulose filter arranges thieving paper, glass fibre element film arranges hemofiltration film; On described detection line, every centimetre of bag is by 160ng mouse-anti people NMP-22 antibody; On described nature controlling line, every centimetre of bag is by 1.0 μ g goat anti-rabbit antibodies;
Described reagent card also comprises shell, and described shell comprises the upper cover and lower cover that link together, covers and be provided with blood sample hole and detection window on described; The position of the corresponding hemofiltration film in position in described blood sample hole; The corresponding detection line in position of described detection window and the position of nature controlling line, make detection line and nature controlling line be in detection window; Described outer casing upper cover detection window right side blank place can be used as the record surface for recording data.
The present embodiment immunofluorescence chromatography detects the preparation method of NMP-22 kit, comprises following operation steps:
1) damping fluid is prepared: get fluorescently-labeled rabbit anti-human NMP-22 antibody 55ng, add 60 μ l, pH=7.3, phosphate concn are in the phosphate buffer of 30mmol/L, mix, obtain damping fluid;
2) reagent card is prepared: get nitrocellulose filter, mark detection line and nature controlling line, then on detection line specking bag by mouse-anti people NMP-22 antibody, on nature controlling line, specking bag is by goat anti-rabbit antibody, then nitrocellulose filter, glass fibre element film, thieving paper, hemofiltration film are overlapped on plastic base plate successively, and cut into required width, then load in the shell be joined together to form by upper cover and lower cover, obtain described reagent card; Wherein outer casing upper cover is provided with blood sample hole and detection window, the position of the corresponding hemofiltration film in position in described blood sample hole, the corresponding detection line in position of described detection window and the position of nature controlling line, make detection line and nature controlling line be in detection window, described outer casing upper cover detection window right side blank place can be used as the record surface of record data;
3) by step 1) damping fluid, the step 2 prepared) reagent card prepared, assembling, obtains described immunofluorescence chromatography and detects NMP-22 kit.
The present embodiment kit containment is under 4 ~ 8 DEG C of environment.
Embodiment 4
The using method of the kit that above-described embodiment 1 ~ 3 provides, concrete operation step is:
I: the pre-service of heparin-binding protein standard items: get 5000U NMP-22 standard items add 1ml without in normal person's freshly voided urine of NMP-22, make mother liquor; With the freshly voided urine without NMP-22, this mother liquor is diluted to respectively the NMP-22 standard solution of 87U/ml, 130U/ml, 80U/ml, 40U/ml, 20U/ml, 10U/ml, 5U/ml, 2.5U/ml, 0.5U/ml;
II: get damping fluid, equilibrate at room temperature 15 minutes, the each 100 μ l of NMP-22 standard solution getting each concentration prepared by step I join respectively 60 μ l balance after damping fluid in, join on the hemofiltration film of reagent card by blood sample hole after mixing, after room temperature water placing flat 10 ~ 15min, reagent card is inserted fluorescence immunoassay readout instrument, setting excitation wavelength is 652nm, wavelength of transmitted light is 668nm, read the fluorescent value of variable concentrations NMP-22 standard items, each concentration NMP-22 standard solution respectively measures 5 times, and calculate the mean value of fluorescent value, be ordinate by the mean value of fluorescent value, the concentration value of corresponding NMP-22 standard solution is that horizontal ordinate does regression curve, draws regression curve equation, obtains the relational expression of fluorescent value and NMP-22 concentration,
III: get damping fluid 60 μ l, equilibrate at room temperature 15 minutes, get testing sample 100 μ l join balance after damping fluid in, join on the hemofiltration film of reagent card by blood sample hole after mixing, room temperature water placing flat 15min, reagent card is inserted fluorescence immunoassay readout instrument, setting excitation wavelength is 652nm, and wavelength of transmitted light is 668nm, reads the fluorescent value of testing sample, this fluorescent value is brought in the relational expression that step II obtains, calculate the concentration of testing sample NMP-22.
Test example
1, the kit using embodiment 1 to provide adopts the method described in embodiment 4 to measure the NMP-22 standard items that concentration is 30U/ml, 82U/ml, 138U/ml respectively, each standard items repeated test 5 times, relative deviation≤9% obtaining the kit detection NMP-22 concentration that embodiment 1 provides calculated, coefficient of variation CV≤10% of same standard items repeated test acquired results, shows that kit accuracy of the present invention is high, reproducible.
2, use the kit that provides of embodiment 1 to adopt the method described in embodiment 4 to measure the concentration (U/ml) of NMP-22 the urine specimen of the 15 routine Healthy Peoples fetched from hospital and 15 routine patient urine samples respectively, its result is as shown in table 1 below:
Table 1
Claims (10)
1. immunofluorescence chromatography detects a NMP-22 kit, it is characterized in that, comprises damping fluid and reagent card;
Described damping fluid is the phosphate buffer containing the anti-human NMP-22 antibody of the fluorescently-labeled rabbit of 0.25 ~ 0.92ng/ μ l;
Described reagent card comprises test strips, described test strips comprises plastic base plate, plastic base plate arranges nitrocellulose filter, the upper left right septum of nitrocellulose filter arranges detection line and nature controlling line, on the left of detection line, nitrocellulose filter is arranged glass fibre element film, on the right side of nature controlling line, nitrocellulose filter arranges thieving paper, glass fibre element film arranges hemofiltration film; Described detection line wrap by mouse-anti people NMP-22 antibody; Described nature controlling line wraps by goat anti-rabbit antibody.
2. immunofluorescence chromatography as claimed in claim 1 detects NMP-22 kit, and it is characterized in that, described reagent card also comprises shell, and described shell comprises the upper cover and lower cover that link together, covers and be provided with blood sample hole and detection window on described; The position of the corresponding hemofiltration film in position in described blood sample hole; The corresponding detection line in position of described detection window and the position of nature controlling line, make detection line and nature controlling line be in detection window.
3. immunofluorescence chromatography as claimed in claim 1 detects NMP-22 kit, and it is characterized in that, on described detection line, the package amount of mouse-anti people NMP-22 antibody is 50 ~ 160ng/cm.
4. immunofluorescence chromatography as claimed in claim 1 detects NMP-22 kit, and it is characterized in that, on described nature controlling line, the package amount of goat anti-rabbit antibody is 0.3 ~ 1.0 μ g/cm.
5. immunofluorescence chromatography as claimed in claim 1 detects NMP-22 kit, and it is characterized in that, pH=6.5 ~ 7.3 of described phosphate buffer, phosphate concn is 30 ~ 80mmol/L.
6. immunofluorescence chromatography as claimed in claim 1 detects NMP-22 kit, and it is characterized in that, the spacing distance between described detection line and nature controlling line is 5mm ~ 10mm.
7. immunofluorescence chromatography as claimed in claim 1 detects NMP-22 kit, and it is characterized in that, the width of described test strips is 3mm ~ 6mm.
8. immunofluorescence chromatography as claimed in claim 1 detects NMP-22 kit, it is characterized in that, the excitation wavelength of the fluorescein that the anti-human NMP-22 antibody of described fluorescently-labeled rabbit adopts is 652nm, and wavelength of transmitted light is 668nm.
9. immunofluorescence chromatography as claimed in claim 8 detects NMP-22 kit, and it is characterized in that, the operation steps of the using method of described kit comprises:
I: the NMP-22 standard items getting variable concentrations join in damping fluid, join on the hemofiltration film of reagent card after mixing, after room temperature water placing flat 10 ~ 15min, reagent card is inserted fluorescence immunoassay readout instrument, setting excitation wavelength is 652nm, wavelength of transmitted light is 668nm, read the fluorescent value of variable concentrations NMP-22 standard items, and the concentration value of fluorescent value and NMP-22 standard items is carried out linear analysis, draw fluorescent value and NMP-22 concentration value typical curve, obtain the concentration relationship formula of fluorescent value and NMP-22;
II: get testing sample and join in damping fluid, join on the hemofiltration film of reagent card after mixing, room temperature water placing flat 10 ~ 15min, reagent card is inserted fluorescence immunoassay readout instrument, setting excitation wavelength is 652nm, and wavelength of transmitted light is 668nm, reads the fluorescent value of testing sample, this fluorescent value is brought in the relational expression that step I obtains, calculate the concentration of testing sample NMP-22.
10. immunofluorescence chromatography as claimed in claim 1 detects a preparation method for NMP-22 kit, it is characterized in that, comprises following operation steps:
1) damping fluid is prepared: get the anti-human NMP-22 antibody of fluorescently-labeled rabbit and add in phosphate buffer, mix, obtain damping fluid;
2) reagent card is prepared: get nitrocellulose filter, mark detection line and nature controlling line, then wrap by mouse-anti people NMP-22 antibody on detection line, nature controlling line wraps by goat anti-rabbit antibody, then nitrocellulose filter, glass fibre element film, thieving paper, hemofiltration film are overlapped on plastic base plate successively, and cut into the reagent card of required width;
3) by step 1) damping fluid, the step 2 prepared) reagent card prepared, assembling, obtains described immunofluorescence chromatography and detects NMP-22 kit.
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Application publication date: 20160511 |