CN105572282A - Composition for assessing nutritive value of egg, application and assessing method - Google Patents
Composition for assessing nutritive value of egg, application and assessing method Download PDFInfo
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Abstract
The invention provides a composition for assessing nutritive value of an egg. The composition contains isoleucine, tyrosine and/or vitamin B1. A method for utilizing the composition to assess the nutritive value of the egg comprises the following steps: detecting if the isoleucine, tyrosine and/or vitamin B1 is contained in the egg or the content of the isoleucine, tyrosine and/or vitamin B1 in the egg; utilizing the composition to symmetrically confirm the height sequence of the nutritive value of the egg, respectively determine the contents of the isoleucine, tyrosine and vitamin B1 in the egg and sequencing from high to low according to the determination results of the three materials; summing the contents of the three determined materials and sequencing from high to low according to the summing result; and lastly, comparing the sequences and confirming the height sequence of the nutritive value of the egg. The composition provided by the invention can be used for assessing the nutritive value of the egg and determining the sequence. The method is simple, quick and accurate and can be used for quantitatively determining.
Description
Technical field
The present invention relates to a kind of application isoleucine assessment egg nutrient to be worth and appraisal procedure, belong to the animal products evaluation areas in agricultural product.
Background technology
Egg is originated as the daily nutrition of the mankind, closely bound up with the life of people.Containing a large amount of nutriments in egg, its medium trace element, thick protein, crude fat, cholesterol, lecithin, vitamin B2 and vitamin A are its main nutritional labelings.These nutrients in egg contribute to promoting neural function, can strengthen memory, prevent memory of elderly person power from failing; Contained trace elements of selenium, zinc, vitamin all has antitumaous effect.So, often edible egg energy abundant nutrition, health care.
The egg now commercially can bought is of a great variety; there is the egg of feeding chicken in largely scale field, also have the extensive thin and small hen egg of putting laying hen in a suitable place to breed and producing, also have artificial egg etc.; the nutritive value of all kinds of egg is uneven, and the nutritive value of egg also more and more obtains the attention of people.Assessment egg nutrient is worth mainly by characterizing the content height of egg Middle nutrition material composition, and then assessment nutritive value.But egg Middle nutrition chemical composition parameter is more, assesses not only workload large simultaneously, and defining standard can be caused to differ, therefore, need a kind of height and then the sign egg nutrient value that are characterized rapidly above-mentioned parameter by one matter or composition badly.
And the nutritive value of egg is relevant to many factors, wherein most important factor comprises feeding manner and layer breed.When layer breed is identical, its nutritive value then depends primarily on the selection of feeding manner.At present, main based on following two kinds of rearing conditions eggs on market: 1) extensive breeding type egg: refer to using free of contamination crops as main feed, using earth's surface insect etc. as auxiliary fodder, raise the egg that chicken produces scattered, such egg is that chicken is laid eggs and the egg that obtains under normal physiological context.It is also called as convolvulate asiabell root, hen egg, Countryside Egg, thin and small hen egg; 2) large-scale cultivation type egg: be with the egg under the chicken of Prof. Du Yucang forage feed is generally the egg under the chicken of mechanization feeding chicken in largely scale field or the nursing of poultry specialist.But some manufacturers are in order to improve economic profit, will originally be that egg under large-scale cultivation condition is labeled as thin and small hen egg, therefore how the egg of the different feeding manner of more effective differentiation can seem particularly important.
Further, if specificity material can be found for assessment of the nutritive value of egg, then the assessment be worth for egg nutrient on market provides shortcut.
Isoleucine Isoleucine, Ile, also known as " isoleucine ", systematic naming method is " α-amino-γ-methylthio-n-butyric acid ".Be one of essential amino acid, belong to the one of aliphatics neutral amino acid.Rhomboid leaf sheet or flat crystal, bitter.Fusing point: 284 DEG C.Water-soluble, be slightly soluble in ethanol.Leucine, isoleucine and valine are all branched-chain amino acids, and they help lend some impetus to the muscle recovery after training.Wherein leucine is the most effective a kind of branched-chain amino acid, effectively can prevent muscle loss, because it can decomposition and inversion be glucose faster.Increasing glucose can prevent musculature impaired, and therefore it is particularly suitable for body-builders.Leucine also promotes bone, skin, and the healing of injured muscle tissue, and doctor advises that postoperative patients takes leucine replenishers usually.The best food source of leucine comprises brown rice, beans, meat, nut, soy meal, and whole wheat.Because it is a kind of requisite amino acid, this means that health can not oneself be produced, can only be obtained by diet.Be engaged in the people of high strength physical exertion and Low protein diet, should consider to take leucine to supplement.Although there is independent supplementary form, had better take in together with valine with isoleucine.Therefore, select mixed type replenishers more convenient.
Vitamin B1, also known as thiamine or antineuritic vitamin or anti beriberi vitamin, is white crystal. its physiological function to improve a poor appetite, and helps digest, and particularly the digestion of carbohydrates, improves mental status; Maintain nerve fiber, muscle, cardiomotility normal, alleviate airsick, seasick, maintain neural normal activity etc.Adult need take in 2mg every day.It is extensively present in the foods such as rice bran, yolk, milk, tomato.
Tyrosine is a kind of aromatic amino acid, and be also one of 20 kinds of Argine Monohydrochlorides being used for synthetic protein, it belongs to nonessential amino acid.A tyrosine hydroxyl more than phenylalanine.The main metabolic pathway of phenylalanine produces tyrosine, phenylalanine hydroxylase.Dysbolism there will be phenyl ketonuria (PKU).Tyrosine is transformed into catecholamine and melanin or exhaustive oxidation and decomposes.Human body lacks tyrosinase, and obstacle appears in B16 cell, claims albinism (albinism).Tyrosine and DOPA, dopamine (relevant to Parkinson's), norepinephrine, in adrenaline synthesis associated tyrosine catabolic process, alcapton decomposition is obstructed and can be occurred alkaptonuria.SH2 domain specific binding phosphotyrosine residue in signal transduction.、
The features such as mass spectrum and chromatographic resolution coupling technique have fast, high sensitivity, high selectivity, especially for the characteristic such as qualitative and quantitative analysis of polytype and minor compound, this technology day by day becomes the important means in analytical chemistry field.From prior art, mass spectrum and hyphenated techniques chromatography are applied in the nutritive value assessment of egg by still not relevant method.The present invention is based on the problems referred to above and a kind of easy solution is provided.
But from prior art, mass spectrum and hyphenated techniques chromatography are applied in the dependent evaluation of egg nutrient value by still not relevant technology.The present invention is based on the problems referred to above and a kind of easy solution is provided.
Summary of the invention
The technical matters that the present invention solves is to provide a kind ofly assesses method that egg nutrient is worth, material and application thereof.
In order to solve the problems of the technologies described above, the present invention is achieved through the following technical solutions:
Identify a composition for egg quality, described composition comprises isoleucine, tyrosine and/or vitamin B1.
Whether wherein said method is by detecting containing isoleucine, tyrosine and/or vitamin B1 in egg, or measures isoleucine, tyrosine and/or vitamin B1 content height in egg.
Utilize described composition systematically to determine a method for egg quality order of quality, it is characterized in that, the method comprises the steps:
(1) measure the content of isoleucine, tyrosine and vitamin B1 in egg respectively, wherein the weight coefficient of isoleucine content is 0.1, and the measurement result according to three kinds of materials sorts from high to low;
(2) content of three kinds of materials step (1) measured is sued for peace, and sorts from high to low according to summed result; And
(3) sequence of step (1) and step (2) is compared, finally determine the order of egg quality quality.
Wherein said method is detected egg by the coupling technique of Ultra Performance Liquid Chromatography piece-rate system (UPLC) with Q-TOF type mass spectrometer (Q-TOFMS/MS).
Identify a method for egg quality, under the following conditions egg detected by the coupling technique of Ultra Performance Liquid Chromatography piece-rate system (UPLC) with Q-TOF type mass spectrometer (Q-TOFMS/MS):
Described high resolution liquid chromatography condition determination is: chromatographic column is WatersAcquityUPLCBEHC18 post; Mobile phase: A phase is methyl alcohol, B phase is 0.05% formic acid water-5mM ammonium acetate solution, gradient elution, and the scheme of gradient elution is as follows: 0-0.1min:0%B-0.0%B; 0.1-3.0min:0.0%B-0.0%B; 3.0-15.0min:0.0%B--100.0%B; 15.0-23.0min:100.0%B--100.0%B; 23.0-23.1min:100.0%B--0.0%B; 23.1-26.0min:0.0%B--0.0%B;
Described Q-TOF type MS/MS measures and adopts positive ion detecting pattern, ESI ion gun;
Mass ions characteristic peak m/z265.1118 detected, retention time t
r=1.6min, mass ions characteristic peak m/z132.1016, retention time t
r=2.35min, and mass ions characteristic peak m/z182.0810 retention time t
r=3.33min.
The present invention also provides the application of described composition in qualification egg quality.
Wherein, whether described application is by detecting containing isoleucine, tyrosine and/or vitamin B1 in egg, or isoleucine, tyrosine and/or vitamin B1 content height.
Wherein, by the coupling technique of Ultra Performance Liquid Chromatography piece-rate system (UPLC) with Q-TOF type mass spectrometer (Q-TOFMS/MS), whether egg is carried out in detection egg containing isoleucine or isoleucine content height.
Advantageous Effects of the present invention is as follows:
The present invention adopts the coupling technique of Ultra Performance Liquid Chromatography piece-rate system (UPLC) and Q-TOF type mass spectrometer (Q-TOFMS/MS), investigate, optimize the step such as collection, data prediction, multivariate data analysis of liquid phase chromatogram condition and the MS detection parameters and data by the collection to egg sample, egg Sample pretreatment method, be intended to obtain the mark distinguishing common egg and thin and small hen egg reliably.
Material of the present invention and method may be used for the nutritive value assessing egg, and the method is simple, rapidly, accurately, and can carry out quantitatively.
Embodiment
For the technique effect absolutely proving technical matters solved by the invention He reach, by following embodiment and embodiment, the present invention is described in detail.
About the assessment of nutritive value, the present invention passes through to chemical composition in egg as crude fat, thick protein, ash content, lecithin, and the parameters such as VB2 and VA characterize.As this area general knowledge, to raise under condition above-mentioned chemical composition content in egg scattered higher due to extensive, and therefore, its nutritive value is higher than the egg under breeding scale condition.The present invention creatively finds that assessment egg nutrient is worth height and can be undertaken by the content measuring wherein isoleucine, the direct standard of height using the content of isoleucine as nutritive value, namely, the nutritive value of the egg that isoleucine content is high is higher than the low egg of isoleucine content, and the nutritive value not containing the egg of isoleucine is minimum.
Medical experiment shows, the quality of putting type egg in a suitable place to breed is significantly higher than artificial feeding type egg, namely put type egg nutrient in a suitable place to breed to be worth higher than artificial feeding type egg, trace it to its cause, because feed lot adopts artificial feeding, feed is single, and containing chemical addition agent in feed, accelerate the frequency of laying eggs of chicken, the nutritive value of egg own is had a greatly reduced quality.And the egg that chicken gives birth to raised scattered by thin and small hen egg, chicken feed, hardly containing chemical addition agent, is mainly main feed with natural crops, such as: grain, vegetables, earth's surface insect etc., chicken is laid eggs under normal physiological context, and nutritive value is quite high.
But, up to the present, although the nutritive value difference of separate sources egg confirms to be as good as, and a lot of scholar also finds trace element, albumen or the content difference of other materials in different egg, but egg nutrient assessment is still and makes comprehensive assessment with multiparameter, multi objective, there is no a kind of can the Specific marker of nutritive value difference of distinguishing different egg.
Based on above-mentioned needs, the present invention provides a kind of method assessed egg nutrient and be worth in a kind of embodiment, and whether the method detects in egg containing isoleucine or isoleucine content height.Can select any chemistry when measuring and whether containing isoleucine and isoleucine content, biological detection method, as long as can to its assessment or quantitatively, be not limited to liquid chromatography mass coupling technique, and also design can measure kit or the test paper of isoleucine or its content further, for the nutritive value of assessment egg.
Based on above-mentioned needs, the present invention provides a kind of composition assessed egg nutrient and be worth in a kind of embodiment, and described composition comprises isoleucine, tyrosine and/or vitamin B1.Preferably, whether described method is by detecting containing isoleucine, tyrosine and/or vitamin B1 in egg, or isoleucine, tyrosine and/or vitamin B1 content height.Can select any chemistry in mensuration, biological detection method, as long as the content of above-mentioned substance or quantitative above-mentioned substance can be measured, be not limited to liquid chromatography mass spectrometric coupling, and also design any kit containing above-mentioned substance or the test paper nutritive value for assessment of egg further.
Egg nutrient is worth a method for sequence to utilize described composition systematically to determine, the method comprises the steps:
(1) measure the content of isoleucine, tyrosine and vitamin B1 in egg respectively, the measurement result according to three kinds of materials sorts from high to low;
(2) content of three kinds of materials step (1) measured is sued for peace, and sorts from high to low according to summed result; And
(3) sequence of step (1) and step (2) is compared, finally determine that egg nutrient is worth the order of height.
Generally, if any one content in above-mentioned three kinds of materials is higher, then can think that the nutritive value of egg to be measured is better, if when three kinds of materials content separately all can not reach obviously high, determine that egg nutrient is worth by comparing the content sum of three kinds of materials.Based on this, determine a kind of method assessed egg nutrient and be worth, first the method measures respectively to the content of above-mentioned three kinds of materials, according to the nutritive value measuring the content obtaining various material and tentatively determine egg, suing for peace after weighting to the content of above-mentioned three kinds of materials afterwards calculates again to egg nutrient sorting out value, and the result finally in conjunction with twice mensuration finally determines that egg nutrient is worth height.
In addition, consider that the impact that three kinds of materials are worth egg nutrient is similar, but each content of material is different, wherein in same sample, the content of isoleucine is generally 10 times of tyrosine and vitamin B1 content, determining that egg nutrient is worth the content only needing to compare each material in sequence, therefore, consider the difference of above-mentioned content, in order to the impact making final assessment result consider the content of above-mentioned three kinds of materials, therefore determine that the weight coefficient of the content of isoleucine is 0.1.
Preferably, described method is detected egg by the coupling technique of Ultra Performance Liquid Chromatography piece-rate system (UPLC) with Q-TOF type mass spectrometer (Q-TOFMS/MS).
Assess the method that egg nutrient is worth, under the following conditions egg detected by the coupling technique of Ultra Performance Liquid Chromatography piece-rate system (UPLC) with Q-TOF type mass spectrometer (Q-TOFMS/MS):
Described high resolution liquid chromatography condition determination is: chromatographic column is WatersAcquityUPLCBEHC18 post; Mobile phase: A phase is methyl alcohol, B phase is 0.05% formic acid water-5mM ammonium acetate solution, gradient elution, and the scheme of gradient elution is as follows: 0-0.1min:0%B-0.0%B; 0.1-3.0min:0.0%B-0.0%B; 3.0-15.0min:0.0%B--100.0%B; 15.0-23.0min:100.0%B--100.0%B; 23.0-23.1min:100.0%B--0.0%B; 23.1-26.0min:0.0%B--0.0%B;
Described Q-TOF type MS/MS measures and adopts positive ion detecting pattern, ESI ion gun;
Mass ions characteristic peak m/z265.1118 detected, retention time t
r=1.6min, mass ions characteristic peak m/z132.1016, retention time t
r=2.35min, and mass ions characteristic peak m/z182.0810 retention time t
r=3.33min.
The present invention also provides the application of described composition in assessment egg nutrient is worth.
Wherein, whether described application is by detecting containing isoleucine, tyrosine and/or vitamin B1 in egg, or isoleucine, tyrosine and/or vitamin B1 content height.
Wherein, by the coupling technique of Ultra Performance Liquid Chromatography piece-rate system (UPLC) with Q-TOF type mass spectrometer (Q-TOFMS/MS), whether egg is carried out in detection egg containing isoleucine or isoleucine content height.
In a kind of embodiment, the present invention also provides a kind of kit assessed egg nutrient and be worth, and described kit contains the reagent of assessment or quantitative composition of the present invention.For assessment of product can be kit, test paper, with reach convenient for assessment of with quantitative object, and can to detect in real time easily.
Experimental example
1.1 sample collection
Egg collection under extensive cultivating condition: the blue brown laying hen in sea raised scattered from area, mountain region (Fengning, Chengde City, Hebei Province Bashing Area farmers'), such chicken mainly with earth's surface insect and grain for food, collect the egg that 20 chickens give birth in phase same amount of time, using such egg as one group, be called " C group ", get 200 pieces, egg; Egg collection under breeding scale condition: in the time same as described above, the laying hen of same kind, in the artificial feeding of Beijing plant, is buied 200 pieces, egg as one group, is called " P group ", and two groups egg is all fresh and preservation condition is consistent.
The mensuration of egg nutrient composition under 1.2 different feeding manners
Respectively get 50 pieces at random respectively from two groups of eggs, prepare to be used for experiment.
Processed by above-mentioned two groups of eggs, measure nutritional labeling parameter wherein, design parameter comprises: ash content, trace element (calcium, phosphorus), protein, amino acid, fat, lecithin, vitamin B2 and vitamin A, and detection method is as follows:
Ash content detection method: with reference to the national standard mensuration of ash content " in the GB/T5009.4-2010 food " method.
Trace element (calcium, phosphorus) detection method: with reference to the national standard mensuration of calcium, iron, zinc, sodium, potassium, magnesium, copper and manganese " in GB5413.21-2010 infant food and the dairy products " method.
Protein detection method: with reference to national standard " mensuration of GB5009.5-2010 Protein in Food " method.
Amino-acid analyzer: with reference to national standard " in GB/T5009.124-2003 food amino acid whose mensuration " method (Beckman121MB type)
Fat detection method: with reference to national standard " mensuration fatty in GB5009.6-2010 food " method.
Lecithin content detection method: phosphovanadomolybdic acid ammonium colourimetry (You Nike UV-2802H type ultraviolet-visible pectrophotometer).
Vitamin B2 detection method of content: high performance liquid chromatography (Waters high performance liquid chromatograph).
Vitamin A content detection method: antimony trichloride colourimetry (You Nike UV-2802H type ultraviolet-visible pectrophotometer).
Two groups of eggs (amounting to 100 pieces) are detected above-mentioned parameter respectively, testing result adopts SPSS12.0 software to carry out t check analysis, result shows, ash content, trace element (calcium, phosphorus), protein, amino acid, fat, lecithin, vitamin B2 and vitamin A under extensive cultivating condition in egg (C group) content be all better than egg (P group) under breeding scale condition, therefore can judge, under extensive cultivating condition, egg nutrient is worth and is better than egg under breeding scale condition.
The mark of the novel assessment nutritive value of 1.3 searching
1.3.1 sample pre-treatments
Get each 50 pieces more at random respectively from two groups of eggs, beating eggs also stirs evenly, and is placed in refrigerator and cooled and hides preservation, to be measured.Take sample 2.0g, be placed in 50ml centrifuge tube, add the formic acid acetonitrile solution 6ml of 1%, vortex mixing 5min, after high speed centrifugation, gets supernatant and is placed in another 50.0ml centrifuge tube; Extract twice and merge extract, extract nitrogen dries up, and adds mobile phase 2.0ml and dissolves, and after high speed centrifugation, 0.45 μm of filtering with microporous membrane, measures for UPLC-MS/MS.
1.3.2 stratographic analysis
Chromatographic column is WatersAcquityUPLCBEHC18 post (100mm × 2.1mm, 1.7 μm); Mobile phase: A phase is methyl alcohol, B phase is 0.05% formic acid water-5mM ammonium acetate solution, gradient elution: 0-0.1min:0%B-0.0%B; 0.1-3.0min:0.0%B-0.0%B; 3.0-15.0min:0.0%B--100.0%B; 15.0-23.0min:100.0%B--100.0%B; 23.0-23.1min:100.0%B--0.0%B; 23.1-26.0min:0.0%B--0.0%B; Flow velocity is 0.25mLmin
-1; Column temperature is 30 DEG C; Sample size is 5 μ L.
1.3.3 mass spectrophotometry
Adopt TripleTOF-4600 type MS/MS mass spectrometer (QSTARTMElite, ABSciex), be furnished with ESI source and AnalystQS2.0 data handling system.Adopt positive ion detecting pattern, ESI ion gun, the gas of use is nitrogen.Scanning of the mass spectrum mode and condition determination as follows:
| Scan pattern | Cation mode |
| TOF mass range (TOF Masses) | 100-1000 |
| Spray voltage (Ionspray Voltage) (IS) | 5500 |
| Gas curtain gas (Curtain Gas) | 25psi |
| Atomization gas (Nebulizer Gas) | 50psi |
| Dry gas (Drying Gas) | 50psi |
| Temperature (TEM) | 550℃ |
| Remove a bunch voltage (Declustering Potential) | 70V |
1.3.4 data analysis
Under these conditions, MarkerView is passed through to the ion obtained in mass spectrophotometry
tMsoftly to analyze, finally find m/z265.1118 (retention time t
r=1.6min), m/z132.1016 (retention time t
r=2.35min), m/z182.0810 (retention time t
r=3.33min) in two groups of eggs, there is significant difference, in C group, content is significantly higher; According to above-mentioned mass spectrometric data, by mass spectrometric data retrieval and analysis, this material of initial guess is vitamin B1, tyrosine and isoleucine.
1.3.5 the structure verification of difference ion
In order to assess said structure, therefrom state's medicine biological product determination research institute buys isoleucine reference substance, and compared by rapid ultra high effect liquid phase chromatogram piece-rate system and Q-TOF type MS/MS instrument, these compounds of final confirmation are respectively vitamin B1, isoleucine and tyrosine, and concrete structure is as follows.
Thiamines (vitamin B1)
Isoleucine
Tyrosine
1.3.6 the determination of mark
By said method, we have found novel mark composition-vitamin B1, isoleucine and the tyrosine that can be used for nutritive value assessment, this mark can be evaluated egg nutrient quickly and accurately and be worth.
The nutritive value of isoleucine assessment egg is described below by specific embodiment.
Embodiment
Embodiment 1:
Get 20 pieces, the egg that the chicken raised scattered in area, mountain region (Yi County, Baoding, Hebei province) gives birth to, be called " A group ", in time simultaneously same as described above, 20 pieces, the egg that artificial forage feed mode plant buys from Hebei, be called " B group ", 20 pieces, the egg that the plant of artificial forage feed mode buys from Harbin, is called " C group ".From egg source and nutritive value decision analysis, significantly, put type A group egg (being commonly called as thin and small hen egg) nutritive value in a suitable place to breed and be better than artificial feeding type B group and C group egg.
Observe from egg outward appearance (volume, weight, color and luster), can find A group egg small volume, weight is general comparatively large less than 50g, B group egg volume, and weight is generally greater than 50g; C group egg is substantially identical with A group in appearance, and from volume and weight, B group is better than A, C group.Be opened in container, particularly egg yellow is obviously yellow compared with other two groups for the color of A group, and other yolk colors of two groups are as broad as long, and thus, A group is better than B, C group.Can be found out by above-mentioned analysis, be difficult to be distinguished its nutritive value by outward appearance.
Above-mentioned sample is carried out sample pre-treatments, stratographic analysis, mass spectrophotometry and data analysis according to the method for 1.2-1.5 in experimental example, and result is as follows:
The content of the vitamin B1 in A group is the content of 3.261ppm, B group egg vitamin B1 is 0.827ppm; A group is apparently higher than B group, and the content of C group egg vitamin B1 is that 0.801ppm, A group nutritive value is the highest, and B group and C group are distinguished not quite, and A group is obviously low.
The content of the tyrosine in A group is the content of 6.265ppm, B group egg tyrosine is 1.663ppm; A group is apparently higher than B group, and the content of C group egg tyrosine is that 2.020ppm, A group nutritive value is the highest, and B group and C group are distinguished not quite, and comparatively A group is obviously low.
The content of the isoleucine in A group is the content of 35.738ppm, B group egg isoleucine is 15.178ppm; A group is apparently higher than B group, and the content of C group egg isoleucine is that 18.711ppm, A group nutritive value is the highest, and B group and C group are distinguished not quite, and comparatively A group is obviously low.
The nutritive value height judging B group and C group egg is difficult to by above-mentioned data, in order to compare the nutritive value height of B group and C group egg, calculate the content of three kinds of materials respectively and compare, the content of B group three kinds of materials and be the content of 0.827ppm+1.663ppm+15.178ppm × 0.1=4.008ppm, C group three kinds of materials and be 0.801ppm+2.020ppm+18.711ppm × 0.1=4.692ppm.By content and relatively can find out, B group is lower than C group, and namely the nutritive value of C group egg is better than B group.
In sum, the sequence that egg nutrient is worth is that A group is optimum, and B group is the most bad, and C group is placed in the middle.Material of the present invention may be used for the nutritive value of assessment egg and may be used for sequential determination, and the method is simple, rapidly, accurately, can carry out quantitatively.
Embodiment 2:
Get 10 pieces, the egg that the chicken raised scattered in area, mountain region (Yi County, Baoding, Hebei province) gives birth to, be called " A group ", in the time simultaneously same as described above, 10 pieces, the common egg buied from Beijing Wal-Mart supermarket, be called " D group ", buy 10 pieces, egg from country fair, be called " E group ".
Observe from egg outward appearance, color and luster, can find that A group egg is smaller, general less than 50g, major part for skin be white, D group egg is larger, is generally greater than 50g, most of for egg skin be rufous; E group egg is substantially identical with D group in appearance.Be opened in container, particularly egg yellow is obviously yellow compared with other two groups for the color of A group, and other yolk colors of two groups are as broad as long.
Above-mentioned sample is carried out sample pre-treatments, stratographic analysis, mass spectrophotometry and data analysis according to the method for 1.2-1.5 in experimental example, and result is as follows:
The content of the vitamin B1 in A group is the content of 3.561ppm, B group egg vitamin B1 is 0.957ppm; A group is apparently higher than D group, and the content of E group egg vitamin B1 is that 1.665ppm, A group nutritive value is the highest, and D group and E group are distinguished not quite, and A group is obviously low.
The content of the tyrosine in A group is the content of 6.465ppm, B group egg tyrosine is 1.863ppm; A group is apparently higher than D group, and the content of E group egg tyrosine is that 1.952ppm, A group nutritive value is the highest, and D group and E group are distinguished not quite, and comparatively A group is obviously low.
The content of the isoleucine in A group is the content of 38.562ppm, B group egg isoleucine is 16.542ppm; A group is apparently higher than D group, and the content of E group egg isoleucine is that 16.065ppm, A group nutritive value is the highest, and D group and E group are distinguished not quite, and comparatively A group is obviously low.
The nutritive value height judging D group and E group egg is difficult to by above-mentioned data, in order to compare the nutritive value height of E group and D group egg, calculate the content of three kinds of materials respectively and compare, the content of D group three kinds of materials and be the content of 0.957ppm+1.863ppm+16.542ppm × 0.1=4.474ppm, E group three kinds of materials and be 1.665ppm+1.952ppm+16.065ppm × 0.1=5.224ppm.By content and relatively can find out, D group is lower than E group, and namely the nutritive value of E group egg is better than D group.
In sum, the sequence that egg nutrient is worth is that A group is optimum, and D group is the most bad, and E group is placed in the middle.Material of the present invention may be used for the nutritive value of assessment egg and may be used for sequential determination, and the method is simple, rapidly, accurately, can carry out quantitatively.
Claims (9)
1. assess the composition that egg nutrient is worth, it is characterized in that, described composition comprises isoleucine, tyrosine and/or vitamin B1.
2. one kind utilizes the method that described in claim 1, composition assessment egg nutrient is worth, whether described method is by detecting containing isoleucine, tyrosine and/or vitamin B1 in egg, or by detecting isoleucine, tyrosine and/or vitamin B1 content height in egg.
3. assess the method that egg nutrient is worth as claimed in claim 2, by the coupling technique of Ultra Performance Liquid Chromatography piece-rate system (UPLC) with Q-TOF type mass spectrometer (Q-TOFMS/MS), egg is detected.
4. utilize composition determination egg nutrient described in claim 1 to be worth a method for height Gauss order, it is characterized in that, the method comprises the steps:
(1) measure the content of isoleucine, tyrosine and vitamin B1 in egg respectively, the measurement result according to three kinds of materials sorts from high to low;
(2) content of three kinds of materials step (1) measured is sued for peace, and wherein the weight coefficient of isoleucine content is 0.1, sorts from high to low according to summed result; And
(3) sequence of step (1) and step (2) is compared, finally determine that egg nutrient is worth the order of height height.
5. assess the method that egg nutrient is worth, it is characterized in that, under the following conditions egg is detected by the coupling technique of Ultra Performance Liquid Chromatography piece-rate system (UPLC) with Q-TOF type mass spectrometer (Q-TOFMS/MS):
Described high resolution liquid chromatography condition determination is: chromatographic column is WatersAcquityUPLCBEHC18 post; Mobile phase: A phase is methyl alcohol, B phase is 0.05% formic acid water-5mM ammonium acetate solution, gradient elution, and the scheme of gradient elution is as follows: 0-0.1min:0%B-0.0%B; 0.1-3.0min:0.0%B-0.0%B; 3.0-15.0min:0.0%B--100.0%B; 15.0-23.0min:100.0%B--100.0%B; 23.0-23.1min:100.0%B--0.0%B; 23.1-26.0min:0.0%B--0.0%B;
Described Q-TOF type MS/MS measures and adopts positive ion detecting pattern, ESI ion gun;
Mass ions characteristic peak m/z265.1118 detected, retention time t
r=1.6min, mass ions characteristic peak m/z132.1016, retention time t
r=2.35min, and mass ions characteristic peak m/z182.0810 retention time t
r=3.33min.
6. the application of composition described in claim 1 in assessment egg nutrient is worth.
7. apply as claimed in claim 6, whether wherein said application is by detecting containing isoleucine, tyrosine and/or vitamin B1 in egg, or isoleucine, tyrosine and/or vitamin B1 content height.
8. whether application as claimed in claims 6 or 7, wherein carried out in detections egg containing isoleucine or isoleucine content just egg by the coupling technique of Ultra Performance Liquid Chromatography piece-rate system (UPLC) and Q-TOF type mass spectrometer (Q-TOFMS/MS).
9. assess the kit that egg nutrient is worth, it is characterized in that, described kit contains the reagent of composition described in assessment or quantitative claim 1.
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| CN104634907A (en) * | 2013-11-11 | 2015-05-20 | 上海交通大学医学院附属瑞金医院 | Application of amino acid molecular combination as stomach cancer marker |
| CN105424854A (en) * | 2015-11-23 | 2016-03-23 | 济南英盛生物技术有限公司 | Method for detecting various water-soluble vitamins in blood sample at same time |
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| CN104634907A (en) * | 2013-11-11 | 2015-05-20 | 上海交通大学医学院附属瑞金医院 | Application of amino acid molecular combination as stomach cancer marker |
| CN105424854A (en) * | 2015-11-23 | 2016-03-23 | 济南英盛生物技术有限公司 | Method for detecting various water-soluble vitamins in blood sample at same time |
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