CN105567844A - Primer pair and method for identifying hericium erinaceus houjie No.2 or large hericium erinaceus - Google Patents
Primer pair and method for identifying hericium erinaceus houjie No.2 or large hericium erinaceus Download PDFInfo
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- CN105567844A CN105567844A CN201610081844.2A CN201610081844A CN105567844A CN 105567844 A CN105567844 A CN 105567844A CN 201610081844 A CN201610081844 A CN 201610081844A CN 105567844 A CN105567844 A CN 105567844A
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Abstract
The invention discloses a primer pair and method for identifying hericium erinaceus houjie No.2 or large hericium erinaceus. The primer pair comprises an upstream primer and a downstream primer, wherein the upstream primer is 5'-ATTAGCTTGACGTACAGACAGTCG-3', and the downstream primer is 5'-TGTTGGAGCAAGGGTGATACGA-3'. According to the primer pair, hericium erinaceus houjie No.2 and large hericium erinaceus share one specific molecular marker. The PCR method adopting the primer pair has the advantages that detection time is short, accuracy is high, judgment is easy and repeatability is high when used for identifying hericium erinaceus houjie No.2 or large hericium erinaceus, and early-stage identification can be achieved quickly.
Description
Technical field
The present invention relates to Molecular Identification technical field, particularly relate to a kind of primer pair and the method for identifying outstanding No. 2 or large hedgehog hydnums of Hericium erinaceus (Bull. Ex Fr.) Pers. monkey.
Background technology
Hericium erinaceus (Bull. Ex Fr.) Pers. (Hericiumerinaceus), cries Hericium erinaceus (Bull. Ex Fr.) Pers. again, only gains the name because exactly liking hedgehog hydnum.Monkey mushroom, hedgehog hydnum, monkey mushroom, is the luxury dish of Chinese tradition, meat is tender, taste is fragrant, tasty.Hericium erinaceus (Bull. Ex Fr.) Pers. is the rare mushroom that a kind of edible medicinal has both, and its sporophore and fermented hypha all can be used as medicine.Along with the raising of living standards of the people, Hericium erinaceus (Bull. Ex Fr.) Pers. also enters average family, and particularly after SARS, the nutrition of Hericium erinaceus (Bull. Ex Fr.) Pers. and pharmaceutical use are familiar with more, and Hericium erinaceus (Bull. Ex Fr.) Pers. market comsupton is anxious to be increased, and the wide market requirement makes artificial culture develop rapidly.
Along with the fast development of Hericium erinaceus (Bull. Ex Fr.) Pers. industry, further urgent to high yield, degeneration-resistant Hericium erinaceus (Bull. Ex Fr.) Pers. germ plasm resource demand.But because Hericium erinaceus (Bull. Ex Fr.) Pers. kind itself has vegetative hereditary property, make homonymus or synonymum on bacterial classification market, xenogenesis kind of the same name, false, inferior strain phenomenon ubiquity, add the degradation phenomena of strain quality, not only greatly compromise the interests of the Hericium erinaceus (Bull. Ex Fr.) Pers. producer and breeder, also have impact on the sustainable and healthy development of Hericium erinaceus (Bull. Ex Fr.) Pers. industry.Therefore, Hericium erinaceus (Bull. Ex Fr.) Pers. cultivar identification system is to closing needs reliably, quickly and easily to set up a set of science.And China is at present to the supervision and management of Hericium erinaceus (Bull. Ex Fr.) Pers. variety and quality, mainly rely on every production technical standard that production aspect is formulated, and not yet obtain key breakthrough at strain quality technological layer, particularly lack the early stage quick identification technology system of Hericium erinaceus (Bull. Ex Fr.) Pers. breeding.
With regard to nationwide, current Hericium erinaceus (Bull. Ex Fr.) Pers. main breed is 15, comprises outstanding No. 2 of monkey, Hericium erinaceus (Bull. Ex Fr.) Pers. 99, Hericium erinaceus (Bull. Ex Fr.) Pers. G2M5.496, Hericium erinaceus (Bull. Ex Fr.) Pers. G2M5.497, Hericium erinaceus (Bull. Ex Fr.) Pers. 920, Hericium erinaceus (Bull. Ex Fr.) Pers. 911, Hericium erinaceus (Bull. Ex Fr.) Pers. 99-1, Hericium erinaceus (Bull. Ex Fr.) Pers. H6, Monkey King, large hedgehog hydnum, hedgehog hydnum No. 6, Changshan Hericium erinaceus (Bull. Ex Fr.) Pers., hedgehog hydnum BJ-5, Fujian Hericium erinaceus (Bull. Ex Fr.) Pers., Hericium erinaceus (Bull. Ex Fr.) Pers. No. 19.To the Antagonistic reaction etc. that the macroscopic view of the discriminating Main Basis hericium mycelium of these Hericium erinaceus (Bull. Ex Fr.) Pers. kinds, bacterium colony and sporophore shape is now examined, occurred between growth characteristics, biochemical reactions and different strains, but because these methods are subject to the impact of environmental factors and physiological situation, and along with kind quantity is on the increase, make cultivar identification more and more difficult.
Since this century, the molecular marking technique of some PCR-based is as RAPD (RandomAmplifiedPolymorphicDNA, randomly amplified polymorphic DNA), ISSR (Inter-SimpleSequenceRepeat, Inter-simple sequence repeat) and SRAP (Sequence-RelatedAmplifiedPolymorphism, SRAP) in succession for the Genetic Diversity of Germplasm of Hericium erinaceus (Bull. Ex Fr.) Pers., genetic distance is studied, but chain for molecule marker and Hericium erinaceus (Bull. Ex Fr.) Pers. proterties, the interracial molecular identificalion research of Hericium erinaceus (Bull. Ex Fr.) Pers. seldom.Moreover, what these molecular marking techniques adopted is total primer, its pcr amplification collection of illustrative plates is complicated, poor repeatability not only, and specificity is not high, therefore and be not suitable for Variety identification, only develop certain stable variety, special DNA fingerprint mark could really for the accurate Rapid identification of this Hericium erinaceus (Bull. Ex Fr.) Pers. kind.
CN101985657A discloses a kind of method utilizing molecular marking technique to identify hedgehog fungus bacterial Monkey King, comprises mycelium culture and collection, the extraction of genomic dna, the detection foundation of SCAR-PCR molecule marker and the detection of SCAR-PCR product.Use the molecular specificity primer Monkey King F2/R2 of this disclosure of the invention to detect primer and carry out pcr amplification, the feature amplified production of hedgehog fungus bacterial Monkey King is 371bp.CN101402994A discloses a kind of method utilizing molecular marking technique to identify hedgehog fungus bacterial monkey outstanding person, similar in concrete steps and CN101985657A, use the molecular specificity primer of this disclosure of the invention to carry out pcr amplification, the feature amplified production of hedgehog fungus bacterial monkey outstanding person is 414bp.
Summary of the invention
The invention provides a kind of primer pair identifying outstanding No. 2 or large hedgehog hydnums of Hericium erinaceus (Bull. Ex Fr.) Pers. monkey, this primer specificity is high, and utilize this primer to carry out PCR qualification, easy and simple to handle, accuracy is high.
Primer pair, comprises upstream primer and downstream primer:
Upstream primer is: 5 '-ATTAGCTTGACGTACAGACAGTCG-3 ',
Downstream primer is: 5 '-TGTTGGAGCAAGGGTGATACGA-3 '.
Sequence amplification polymorphism (Sequence-relatedAmplifiedPolymorphism, SRAP) molecular marking technique of being correlated with is utilized to carry out exploratory study to the molecular identificalion of Hericium erinaceus (Bull. Ex Fr.) Pers. main breed.Extract the mycelia genomic dna of each Hericium erinaceus (Bull. Ex Fr.) Pers. kind as template, with a large amount of SRAP random primer to carrying out pcr amplification, amplified production agarose gel electrophoresis detects, and finally screening obtains having the primer a pair of differential band (only Hericium erinaceus (Bull. Ex Fr.) Pers. monkey outstanding No. 2 and large hedgehog hydnum have and all the other kinds do not have).This differential band is carried out cut glue to reclaim, be then connected on cloning vector and send to order-checking, gained DNA fragmentation size is 620bp, and its base sequence is as shown in SEQIDNo.1, and this DNA fragmentation is monkey outstanding No. 2 and large hedgehog hydnum public specificity DNA fragmentation.Described primer pair is obtained by the design of this DNA fragment specific.
With Hericium erinaceus (Bull. Ex Fr.) Pers. genomic dna for template, utilize described primer to carry out pcr amplification, only monkey outstanding person No. 2 and large hedgehog hydnum Absorbable organic halogens obtain the DNA fragment specific of 620bp size, and other Hericium erinaceus (Bull. Ex Fr.) Pers. kinds all can not obtain this DNA fragment specific.
Invention further provides the application of described primer pair in outstanding No. 2 or large hedgehog hydnums of qualification Hericium erinaceus (Bull. Ex Fr.) Pers. monkey.
Present invention also offers the application of described primer pair in the test kit of outstanding No. 2 or large hedgehog hydnums of characterization Hericium erinaceus (Bull. Ex Fr.) Pers. monkey.Described test kit comprises described primer pair, DNA extraction reagent, the related reagent such as polysaccharase, damping fluid, dNTP that PCR is used.
Present invention also offers the method for outstanding No. 2 or large hedgehog hydnums of described primer qualification Hericium erinaceus (Bull. Ex Fr.) Pers. monkey, comprise the following steps:
(1) DNA extraction,
(2) pcr amplification,
(3) electrophoresis detection,
If the DNA band of 620bp size appears in electrophoresis detection result, then Hericium erinaceus (Bull. Ex Fr.) Pers. kind to be measured is outstanding No. 2 or large hedgehog hydnums of Hericium erinaceus (Bull. Ex Fr.) Pers. monkey, otherwise then no.
The inventive method key is the selection of amplimer, as DNA extraction, PCR reaction system and reaction conditions, and electrophoresis detection, all can carry out according to this area ordinary method.
Preferably, PCR reaction system of the present invention is composed as follows:
Wherein 2 × PowerTaqPCRMasterMix is purchased from Beijing hundred Tyke Bioisystech Co., Ltd.
Described pcr amplification condition is: 94 DEG C of denaturation 7min; 94 DEG C of sex change 45min, 59 DEG C of annealing 45s, 72 DEG C extend 2min, totally 30 circulations; 72 DEG C extend 7min; 4 DEG C of insulations.
The DNA fragmentation of the 620bp utilizing outstanding No. 2 or large hedgehog hydnums of described primer pair amplifies Hericium erinaceus (Bull. Ex Fr.) Pers. monkey to obtain can as Hericium erinaceus (Bull. Ex Fr.) Pers. monkey outstanding No. 2 and large hedgehog hydnum public specificity DNA fragmentation, and base sequence is as shown in SEQIDNo.1.
Present invention also offers described DNA fragment specific as the application of molecule marker in outstanding No. 2 or large hedgehog hydnums of qualification Hericium erinaceus (Bull. Ex Fr.) Pers. monkey.
Certainly, on the basis of described DNA fragment specific, described primer pair is carried out to increase or the minimizing of Individual base, also only have outstanding No. 2 of monkey can stablize acquisition specific fragment through pcr amplification test, also may be used for outstanding No. 2 or large hedgehog hydnums of qualification monkey.
Primer pair of the present invention is Hericium erinaceus (Bull. Ex Fr.) Pers. monkey outstanding No. 2 and large hedgehog hydnum public specificity molecule marker, utilize the PCR method comprising described primer pair to identify outstanding No. 2 or large hedgehog hydnums of Hericium erinaceus (Bull. Ex Fr.) Pers. monkey, have that detection time is short, accuracy is high, easy judgement, the advantage such as reproducible, and can Early Identification fast be carried out.
Accompanying drawing explanation
Fig. 1 carries out pcr amplification result electrophorogram for utilizing outstanding No. 2 of molecular marker primer pair Hericium erinaceus (Bull. Ex Fr.) Pers. monkey of the present invention, large hedgehog hydnum and other Hericium erinaceus (Bull. Ex Fr.) Pers. kinds, wherein 1 is outstanding No. 2 of Hericium erinaceus (Bull. Ex Fr.) Pers. monkey, 10 is large hedgehog hydnum, and 2 ~ 9,11 ~ 15 is other Hericium erinaceus (Bull. Ex Fr.) Pers. kind, and M is DL2000DNAMarker.
Embodiment
Embodiment 1
SRAP molecular marking technique is utilized to carry out exploratory study to the molecular identificalion of 15 kinds of Hericium erinaceus (Bull. Ex Fr.) Pers. main breeds.Extract the mycelia genomic dna of each Hericium erinaceus (Bull. Ex Fr.) Pers. kind as template, with a large amount of SRAP random primer to carrying out pcr amplification, amplified production agarose gel electrophoresis detects, and finally screening obtains having the primer a pair of differential band (only Hericium erinaceus (Bull. Ex Fr.) Pers. monkey outstanding No. 2 and large hedgehog hydnum have and all the other kinds do not have).This differential band is carried out cut glue to reclaim, be then connected on cloning vector and send to order-checking, gained DNA fragmentation size is 620bp, and its base sequence is as shown in SEQIDNo.1, and this DNA fragmentation is monkey outstanding No. 2 and large hedgehog hydnum public specificity DNA fragmentation.
For gained monkey outstanding No. 2 and large hedgehog hydnum public specificity DNA fragmentation design primer pair, the sequence of primer pair is:
Upstream primer: 5 '-ATTAGCTTGACGTACAGACAGTCG-3 '
Downstream primer: 5 '-TGTTGGAGCAAGGGTGATACGA-3 ',
Primer is synthesized by Shanghai biotechnology company limited.
With the genomic dna of 15 kinds of main breed Hericium erinaceus (Bull. Ex Fr.) Pers. for template, utilize described primer pair to carry out pcr amplification, only monkey outstanding person No. 2 and large hedgehog hydnum Absorbable organic halogens obtain the DNA fragment specific of 620bp size, and other Hericium erinaceus (Bull. Ex Fr.) Pers. kinds all can not obtain this DNA fragment specific.
Embodiment 2
(1) extraction of Hericium erinaceus (Bull. Ex Fr.) Pers. fine breed gene group DNA
Get hericium mycelium 0.03g to be measured, add liquid nitrogen thoroughly to grind, utilize novel quick-speed plant genome DNA extracting reagent kit (DP3111, BioTeke, Beijing hundred Tyke Bioisystech Co., Ltd) extract genomic dna, obtain the genomic dna crude extract of Hericium erinaceus (Bull. Ex Fr.) Pers. breeding.Agarose gel electrophoresis and DNA/RNA ultraviolet spectrophotometer (NanodropTechnologies, USA) is used to detect the integrity of gained genomic dna crude extract, purity and concentration.The DNA sample of OD260/OD280>1.8 is used for subsequent PCR amplification.DNA extraction thing is for subsequent use in-20 DEG C of refrigerator storages.
(2) pcr amplification
PCR reaction solution forms: 2 × PowerTaqPCRMasterMix10 μ L, 10 μMs of upstream and downstream special primers are to each 1.5 μ L, 20ng/ μ L template DNA 3 μ L, ddH
2o4 μ L.
Amplified reaction carries out on LifeECO type amplification instrument (Bioer, Hangzhou BIOER Technology Co., Ltd).Amplification condition: 94 DEG C of denaturation 7min; 94 DEG C of sex change 45min, 59 DEG C of annealing 45s, 72 DEG C extend 2min, totally 30 circulations; 72 DEG C extend 7min; 4 DEG C of insulations.
(3) agarose gel electrophoresis runs glue detection
Get step (2) pcr amplification product 5 μ L, mix with 1 μ L0.25% bromjophenol blue damping fluid, point sample is in the sepharose loading hole of 1.5%, and in 1 × TAE damping fluid, under 5V/cm voltage, leakage of electricity is swum.After electrophoresis terminates, dye 30 minutes in containing the aqueous solution of 0.5 μ g/mLEB, then use the automatic gel image analysis instrument (ChemiDocXRSimagingsystem, Bio-Rad, Hercules, CA, USA) of U.S. Bole to carry out observing and taking a picture.
According to the method described above, shown in his-and-hers watches one, 15 Hericium erinaceus (Bull. Ex Fr.) Pers. breedings carry out Testing and appraisal respectively, the results are shown in Figure 1.
Table 1 Hericium erinaceus (Bull. Ex Fr.) Pers. breeding numbering and title.
As shown in Figure 1, be numbered 1 monkey outstanding No. 2 and be numbered 10 large hedgehog hydnum in amplified clear bright, a specific DNA band that molecular size range is 620bp, and the Hericium erinaceus (Bull. Ex Fr.) Pers. breeding of all the other numberings, special DNA band there are no size 620bp produces, other non-object band is not had to produce yet, the molecular specificity marker that visible the present invention develops is for the discriminating of outstanding No. 2 or large hedgehog hydnums of monkey, and its stability, specificity are very high.
Claims (8)
1. primer pair, comprises upstream primer and downstream primer, it is characterized in that,
Upstream primer is: 5 '-ATTAGCTTGACGTACAGACAGTCG-3 ',
Downstream primer is: 5 '-TGTTGGAGCAAGGGTGATACGA-3 '.
2. the application of primer pair in outstanding No. 2 or large hedgehog hydnums of qualification Hericium erinaceus (Bull. Ex Fr.) Pers. monkey as claimed in claim 1.
3. the application of primer pair in the test kit of outstanding No. 2 or large hedgehog hydnums of characterization Hericium erinaceus (Bull. Ex Fr.) Pers. monkey as claimed in claim 1.
4. utilize primer pair as claimed in claim 1 to identify a method for outstanding No. 2 or large hedgehog hydnums of Hericium erinaceus (Bull. Ex Fr.) Pers. monkey, it is characterized in that, comprise the following steps:
(1) DNA extraction,
(2) pcr amplification,
(3) electrophoresis detection,
If the DNA band of 620bp size appears in electrophoresis detection result, then Hericium erinaceus (Bull. Ex Fr.) Pers. kind to be measured is outstanding No. 2 or large hedgehog hydnums of Hericium erinaceus (Bull. Ex Fr.) Pers. monkey.
5. method as claimed in claim 4, it is characterized in that, described pcr amplification reaction system is:
6. method as claimed in claim 4, it is characterized in that, described pcr amplification condition is:
94 DEG C of denaturation 7min; 94 DEG C of sex change 45min, 59 DEG C of annealing 45s, 72 DEG C extend 2min, totally 30 circulations; 72 DEG C extend 7min; 4 DEG C of insulations.
7. Hericium erinaceus (Bull. Ex Fr.) Pers. monkey outstanding No. 2 and large hedgehog hydnum public specificity DNA fragmentation, called after SCAR1, is characterized in that, sequence is as shown in SEQIDNo.1.
8. outstanding No. 2 of Hericium erinaceus (Bull. Ex Fr.) Pers. monkey as claimed in claim 7 is identifying the application in outstanding No. 2 or large hedgehog hydnums of Hericium erinaceus (Bull. Ex Fr.) Pers. monkey with large hedgehog hydnum public specificity DNA fragmentation as molecule marker.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244701A (en) * | 2016-08-25 | 2016-12-21 | 黑龙江省林副特产研究所 | The structure of a kind of Hericium erinaceus (Bull. Ex Fr.) Pers. DNA bar code and authentication method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008101343A1 (en) * | 2007-02-21 | 2008-08-28 | University Of Manitoba | Identification of a candidate blackleg (leptospherea maculans) resistance gene lepr3 in canola (brassica napus) |
| CN101402995A (en) * | 2008-11-18 | 2009-04-08 | 福建农林大学 | Method for technical evaluation of hedgehog fungus bacterial Houwang with numerator mark |
| CN101402994A (en) * | 2008-11-18 | 2009-04-08 | 福建农林大学 | Method for technical evaluation of hedgehog fungus Houjie bacterial with numerator mark |
| CN101985657A (en) * | 2008-11-18 | 2011-03-16 | 福建农林大学 | Method for identifying hericium erinaceus strain king by utilizing molecule marker technology |
-
2016
- 2016-02-05 CN CN201610081844.2A patent/CN105567844B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008101343A1 (en) * | 2007-02-21 | 2008-08-28 | University Of Manitoba | Identification of a candidate blackleg (leptospherea maculans) resistance gene lepr3 in canola (brassica napus) |
| CN101402995A (en) * | 2008-11-18 | 2009-04-08 | 福建农林大学 | Method for technical evaluation of hedgehog fungus bacterial Houwang with numerator mark |
| CN101402994A (en) * | 2008-11-18 | 2009-04-08 | 福建农林大学 | Method for technical evaluation of hedgehog fungus Houjie bacterial with numerator mark |
| CN101985657A (en) * | 2008-11-18 | 2011-03-16 | 福建农林大学 | Method for identifying hericium erinaceus strain king by utilizing molecule marker technology |
Non-Patent Citations (4)
| Title |
|---|
| LI G等: ""Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica"", 《THEORETICAL & APPLIED GENETICS》 * |
| 刘麒等: ""猴头菌DNA提取条件及SRAP- PCR体系的优化"", 《吉林农业大学学报》 * |
| 王玲等: "《动物遗传育种学实验教程》", 31 October 2015 * |
| 郭仰东等: "《植物生物技术实验教程》", 30 June 2015 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244701A (en) * | 2016-08-25 | 2016-12-21 | 黑龙江省林副特产研究所 | The structure of a kind of Hericium erinaceus (Bull. Ex Fr.) Pers. DNA bar code and authentication method |
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