CN105565512A - Bioelectrochemical system functional bacterium domestication method for treating toxic organic wastewater - Google Patents
Bioelectrochemical system functional bacterium domestication method for treating toxic organic wastewater Download PDFInfo
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- CN105565512A CN105565512A CN201610152406.0A CN201610152406A CN105565512A CN 105565512 A CN105565512 A CN 105565512A CN 201610152406 A CN201610152406 A CN 201610152406A CN 105565512 A CN105565512 A CN 105565512A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M8/00—Fuel cells; Manufacture thereof
- H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
- C02F2101/345—Phenols
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
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- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
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- Organic Chemistry (AREA)
- Water Treatment By Electricity Or Magnetism (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention relates to a bioelectrochemical system functional bacterium domestication method for treating toxic organic wastewater. The method comprises the following steps: establishing a conventional microbial fuel cell, wherein the conventional microbial fuel cell adopts a single-electrode-chamber structure, carbon cloth electrodes are respectively arranged on both sides of the electrode chamber, glucose and oxygen are used as an electron donor and a receptor, and a phenol solution is used as a toxic substance; preparing a 1000 mg/L glucose nutrient solution and a 100 mg/L phenol solution, adding the two solutions into the microbial fuel cell electrode chamber according to the volume ratio, and gradually increasing the content of the toxic pollutant in the solution to be treated, thereby avoiding the complete inhibition on the biological oxidation activities of the productivity functional bacterium; and finally, carrying out analysis by voltage output and functional bacterium scanning electron microscope morphological identification. By adopting the bioelectrochemical system for treating the toxic wastewater, the method can enhance the adaptability and treatment efficiency of the bioelectrochemical system for treating toxic wastewater, shortens the research period, and enhances the adaptability of the bioelectrochemical system for treating wastewater.
Description
Technical field
The present invention relates to toxic wastewater energy process field, particularly a kind of bioelectrochemical system function yeast acclimation method processing poisonous organic wastewater.
Background technology
Scientific-technical progress promotes industrial development, while create beneficial result meets material requisite, make increasing organic pollutant entered environment, comprising the novel of wide variety and toxic pollutant, these pollutents reach certain level through long term accumulation, environmental organism can be made to be poisoned, and potential hazard cannot all be estimated.Take activated sludge process as the conventional waste water treatment technology at substantial energy and the water treatment agent of representative, in process toxic wastewater, there is certain limitation.
Bioelectrochemical system is wide concerned waste water energy treatment technology in recent years, is using microorganism as organic pollutant in biological catalyst degrading waste water, the converts chemical energy in organic pollutant is become electric energy simultaneously, complete the process of waste water energy.This technology does not need extra power and water treatment agent input, is subject to people's attention gradually, is expected to the deficiency making up conventional waste water treatment technology at resource circulation utilization and water treatment field.Bioelectrochemical system progress is very fast, develops broad variety.Its reactive system design and configuration varied, ultimate principle is consistent.For the microbiological fuel cell of routine, its configured is made up of cathode compartment and anolyte compartment usually, fills electrode solution in electrode vessel, and place negative electrode and anode respectively, with production capacity function yeast on anode, reactive system is connected with electrical appliance by external circuit, forms loop.Anolyte compartment to degrade place usually used as wastewater organic pollutant, organic pollutant produces electronics and proton through bio-oxidation, proton enters external circuit through anode, negative electrode is arrived by electrical appliance, proton enters cathode compartment through proton exchange membrane, and electronics, proton and electron acceptor(EA) complete reduction reaction at negative electrode.In the process, the transfer of electronics by energy transport to electrical appliance.Deriving technology based on this technology, can be applied to wide field.
Production capacity function yeast plays a key effect in the process, production capacity function yeast utilizes the metabolic process of self to complete the decomposition of organic pollutant in bioelectrochemical system, this metabolic process is subject to the impact of microbe survival environment simultaneously, both there are some researches show that production capacity function yeast better can complete the treatment of Organic Wastewater such as sanitary sewage and recovered energy, and for the toxic wastewater containing biological inhibition effect, the metabolism production capacity effect of function yeast will be suppressed, and then affect decomposition and the production capacity of toxic pollutant, bioelectrochemical system is collapsed.Organic pollutant is oxidation degradation in bioelectrochemical system, and biological oxido reduction active centre is embedded in redox protein matter, reduces redox active, hinders the lifting of organic pollutant degradation efficiency.
Research display, microorganism electrochemical system may be used for the process of toxic wastewater energy, and need the major limiting factors overcome to be the restraining effect of toxic substance to bio-oxidation, this requirement realizes bioelectrochemical system production capacity function yeast to the domestication of toxic wastewater.
Summary of the invention
The object of the invention is to for above-mentioned existing problems, provide a kind of bioelectrochemical system function yeast acclimation method processing poisonous organic wastewater, the method can realize toxic pollutant degraded, and reclaims the energy, avoids the use of the water treatment agent of energy consumption.。
Technical scheme of the present invention:
Process a bioelectrochemical system function yeast acclimation method for poisonous organic wastewater, step is as follows:
1) set up conventional microbiological fuel cell, adopt single electrode cell structure, carbon cloth electrode is placed in electrode vessel both sides respectively, using glucose and oxygen as electron donor and acceptor, using phenol solution as toxic substance;
2) dextrose broth of 1000mg/L and the phenol solution of 100mg/L is prepared, in subsequent step, two kinds of solution to be added in electrode of microbial fuel cell room and the content of incremental raising toxic pollutant in pending liquid according to volume ratio, avoids production capacity function yeast biooxidation activity to suppress completely;
3) stable microbiological fuel cell, electrode solution, in the starting stage, keeps 100% dextrose broth;
4), after voltage stabilization to be output, electrode solution composition changes 20% phenol and 80% dextrose broth into;
5), after voltage stabilization to be output, electrode solution composition changes 50% phenol and 50% dextrose broth into;
6), after voltage stabilization to be output, electrode solution composition changes 80% phenol and 20% dextrose broth into;
7), after voltage stabilization to be output, electrode solution composition changes 100% phenol solution into;
8) by voltage export and function yeast scanning electron microscope identification of morphology analysis, electrode solution composition be 80% phenol and 20% dextrose broth time, anode function bacterium is not subject to toxic organic pollutant and obviously suppresses.
The invention has the beneficial effects as follows:
The method adopts bioelectrochemical system process toxic wastewater method, bioelectrochemical system process toxic wastewater adaptability and processing efficiency can be improved, shorten research cycle, directed can tame out the function yeast can degraded containing specific toxic organic pollutant waste water; Toxic substance is avoided to suppress the effect of production capacity function yeast biological metabolism; By the process of poisonous organic wastewater energy, improve the adaptability of bioelectrochemical system process waste water.
Accompanying drawing explanation
Fig. 1 is that anode surface function yeast is normally adhered in toxic substance-phenol solution volume ratio 80% situation.
Embodiment
Below in conjunction with specific embodiment, such scheme is described further.Embodiment does not lie in for illustration of the present invention and limits the scope of the invention.The implementation condition adopted in embodiment can carry out adjusting and changing according to actual conditions.
Embodiment:
Process a bioelectrochemical system function yeast acclimation method for poisonous organic wastewater, step is as follows:
1) set up conventional microbiological fuel cell, adopt single electrode cell structure, carbon cloth electrode is placed in electrode vessel both sides respectively, using glucose and oxygen as electron donor and acceptor, using phenol solution as toxic substance;
2) dextrose broth of 1000mg/L and the phenol solution of 100mg/L is prepared, in subsequent step, two kinds of solution to be added in electrode of microbial fuel cell room and the content of incremental raising toxic pollutant in pending liquid according to volume ratio, avoids production capacity function yeast biooxidation activity to suppress completely;
3) stable microbiological fuel cell, electrode solution, in the starting stage, keeps 100% dextrose broth;
4), after voltage stabilization to be output, electrode solution composition changes 20% phenol and 80% dextrose broth into;
5), after voltage stabilization to be output, electrode solution composition changes 50% phenol and 50% dextrose broth into;
6), after voltage stabilization to be output, electrode solution composition changes 80% phenol and 20% dextrose broth into;
7), after voltage stabilization to be output, electrode solution composition changes 100% phenol solution into;
8) analyzed by voltage output and function yeast scanning electron microscope identification of morphology, electrode solution composition be 80% phenol and 20% dextrose broth time, anode function bacterium is not subject to toxic organic pollutant and obviously suppresses, and voltage exports normal, and during 100% phenol solution, function yeast comes off from anode.
Fig. 1 is that anode surface function yeast is normally adhered in toxic substance-phenol solution volume ratio 80% situation.Show in figure, according to this acclimation method, microorganism electrochemical system can process higher concentration poisonous organic wastewater.
Claims (1)
1. process a bioelectrochemical system function yeast acclimation method for poisonous organic wastewater, it is characterized in that step is as follows:
1) set up conventional microbiological fuel cell, adopt single electrode cell structure, carbon cloth electrode is placed in electrode vessel both sides respectively, using glucose and oxygen as electron donor and acceptor, using phenol solution as toxic substance;
2) dextrose broth of 1000mg/L and the phenol solution of 100mg/L is prepared, in subsequent step, two kinds of solution to be added in electrode of microbial fuel cell room and the content of incremental raising toxic pollutant in pending liquid according to volume ratio, avoids production capacity function yeast biooxidation activity to suppress completely;
3) stable microbiological fuel cell, electrode solution, in the starting stage, keeps 100% dextrose broth;
4), after voltage stabilization to be output, electrode solution composition changes 20% phenol and 80% dextrose broth into;
5), after voltage stabilization to be output, electrode solution composition changes 50% phenol and 50% dextrose broth into;
6), after voltage stabilization to be output, electrode solution composition changes 80% phenol and 20% dextrose broth into;
7), after voltage stabilization to be output, electrode solution composition changes 100% phenol solution into;
8) by voltage export and function yeast scanning electron microscope identification of morphology analysis, electrode solution composition be 80% phenol and 20% dextrose broth time, anode function bacterium is not subject to toxic organic pollutant and obviously suppresses.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1874040A (en) * | 2005-06-03 | 2006-12-06 | 清华大学 | Single pond type microbiological cell by using organic wastewater as fuel |
| CN101483251A (en) * | 2009-02-02 | 2009-07-15 | 汕头大学 | Microbiological fuel cell for wastewater treatment |
| CN103364469A (en) * | 2013-05-31 | 2013-10-23 | 内蒙古科技大学 | Device and method for quickly measuring BOD (biochemical oxygen demand) based on microbial electrolysis cell technology |
| CN103427101A (en) * | 2012-05-22 | 2013-12-04 | 北京大学深圳研究生院 | Method for generating electric power from waste water by utilization of microbial fuel cell and for boosting voltage and storing |
| CN104150607A (en) * | 2014-07-30 | 2014-11-19 | 华南理工大学 | Device and method for degrading phenol and ammonia nitrogen simultaneously by virtue of microbial fuel cell |
| KR101530527B1 (en) * | 2014-02-21 | 2015-06-30 | 순천대학교 산학협력단 | Microbial fuel cell |
| CN105304923A (en) * | 2015-09-25 | 2016-02-03 | 哈尔滨理工大学 | Method for increasing energy utilization rate of phenol-degradable microbial fuel cell |
-
2016
- 2016-03-17 CN CN201610152406.0A patent/CN105565512A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1874040A (en) * | 2005-06-03 | 2006-12-06 | 清华大学 | Single pond type microbiological cell by using organic wastewater as fuel |
| CN101483251A (en) * | 2009-02-02 | 2009-07-15 | 汕头大学 | Microbiological fuel cell for wastewater treatment |
| CN103427101A (en) * | 2012-05-22 | 2013-12-04 | 北京大学深圳研究生院 | Method for generating electric power from waste water by utilization of microbial fuel cell and for boosting voltage and storing |
| CN103364469A (en) * | 2013-05-31 | 2013-10-23 | 内蒙古科技大学 | Device and method for quickly measuring BOD (biochemical oxygen demand) based on microbial electrolysis cell technology |
| KR101530527B1 (en) * | 2014-02-21 | 2015-06-30 | 순천대학교 산학협력단 | Microbial fuel cell |
| CN104150607A (en) * | 2014-07-30 | 2014-11-19 | 华南理工大学 | Device and method for degrading phenol and ammonia nitrogen simultaneously by virtue of microbial fuel cell |
| CN105304923A (en) * | 2015-09-25 | 2016-02-03 | 哈尔滨理工大学 | Method for increasing energy utilization rate of phenol-degradable microbial fuel cell |
Non-Patent Citations (2)
| Title |
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| 范平等: "不同驯化方式对以苯酚为基质的微生物燃料电池产电性能的影响", 《环境工程学报》 * |
| 陈柳柳等: "微生物燃料电池对苯酚的降解及其产电性能", 《化工环保》 * |
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Application publication date: 20160511 |