CN105548313A - Modified electrode for detecting low-concentration dopamine and making method and application thereof - Google Patents
Modified electrode for detecting low-concentration dopamine and making method and application thereof Download PDFInfo
- Publication number
- CN105548313A CN105548313A CN201610005822.8A CN201610005822A CN105548313A CN 105548313 A CN105548313 A CN 105548313A CN 201610005822 A CN201610005822 A CN 201610005822A CN 105548313 A CN105548313 A CN 105548313A
- Authority
- CN
- China
- Prior art keywords
- electrode
- ppy
- modified
- polypyrrole
- graphene oxide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/308—Electrodes, e.g. test electrodes; Half-cells at least partially made of carbon
Abstract
The invention discloses a modified electrode for detecting low-concentration dopamine. The modified electrode is a glassy carbon electrode modified by polyamphoteric ionic liquid/polypyrrole/graphene oxide (PVIPS/PPy/GO); specifically, the glassy carbon electrode serves as a substrate electrode, and a PPy/GO nanosheet modified by the polyamphoteric ionic liquid is attached to the glassy carbon electrode. The modified electrode can detect low-concentration dopamine and has the advantages of high selectivity, stability and anti-disturbance performance, a wide linear range and the like.
Description
Technical field
The invention belongs to electrochemical analysis field, be specifically related to a kind ofly detect modified electrode of low concentration dopamine and its preparation method and application.
Background technology
Dopamine (DA) is a kind of Catecholamines Neurotransmitters in Blood be present in nerve fiber and body fluid, is by the important chemical substance of helping cell transmission pulse in mammalian central nervous system.Its concentration change in body and cerebration have direct relation, and in brain, dopamine neuronal functional disturbance is the major reason of parkinsonism and other class mental illnesses.Therefore significant to the research of its assay method.
The method of current mensuration dopamine is main mainly with this few class of electrochemical methods, high performance liquid chromatography, fluorescent spectrometry and chemoluminescence method.But sample pretreatment is complicated and detection sensitivity is low, limits the application in reality detects of high performance liquid chromatography, chemoluminescence method and fluorescent spectrometry respectively.And electrochemical methods has cheap, highly sensitive, simple to operate, the fast advantage of reaction velocity, be widely used.Therefore develop a kind of chemically modified electrode that can detect low concentration dopamine neuro-physiology, related drugs are controlled and medical diagnosis on disease important in inhibiting.
Summary of the invention
The object of this invention is to provide and a kind ofly detect modified electrode of low concentration dopamine and its preparation method and application, the modified electrode of this detection low concentration dopamine take glass-carbon electrode as basal electrode, polypyrrole/graphene oxide (PPy/GO) nanometer sheet that the poly-zwitter-ion liquid of attachment is modified on glass-carbon electrode, because poly-zwitter-ion liquid surface has-SO
3-existence, change the charge property of material surface, optimize electric transmission pattern, this nanometer material modified electrode is made to show the wider range of linearity (40-1220nM) in detection low concentration DA, lower detection limit (17.3nM) and preferably selectivity and sensitivity, therefore this modified electrode can detect low concentration dopamine, has the advantages such as higher selectivity, stability, interference free performance and the wider range of linearity.
It is as follows that the present invention realizes the technical scheme that above-mentioned purpose adopts:
A kind of modified electrode detecting low concentration dopamine, described modified electrode is the glass-carbon electrode that poly-zwitter-ion liquid/polypyrrole/graphene oxide (PVIPS/PPy/GO) is modified, being specially with glass-carbon electrode is basal electrode, polypyrrole/graphene oxide (PPy/GO) nanometer sheet that the poly-zwitter-ion liquid of attachment is modified on glass-carbon electrode.
Described a kind of modified electrode detecting low concentration dopamine, described poly-zwitter-ion liquid is poly-1-vinyl-3-propyl sulfonic acid imidazole salts (VIPS).
Detect a preparation method for the modified electrode of low concentration dopamine, comprise the steps:
(1) preparation of polypyrrole/graphene oxide (PPy/GO) nanometer sheet
Under the condition of ultrasonic radiation, the polymerization of pyrroles's in-situ chemical is, on the stannic oxide/graphene nano sheet that Hummers legal system is standby, to obtain polypyrrole/graphene oxide (PPy/GO) nanometer sheet;
(2) preparation of poly-zwitter-ion liquid/polypyrrole/graphene oxide (PVIPS/PPy/GO) nanometer sheet
1-vinyl-3-propane sulfonic acid base imidazole salts (VIPS) and azoisobutyronitrile (AIBN) are scattered in ethanol, N-vinyl imidazole type ionic liquid/polypyrrole/graphene oxide (ILs/PPy/GO) nanometer sheet is added again in system, ultrasonic disperse, obtains potpourri;
By potpourri in oil bath pan, condensing reflux reaction under nitrogen sustainable protection;
After reaction terminates, with distilled water and ethanol, condensing reflux reaction product is repeatedly washed, centrifugal, by centrifugal rear products therefrom vacuum drying 24h, finally obtain black and gather zwitter-ion liquid/polypyrrole/graphene oxide (PVIPS/PPy/GO) nanometer sheet;
(3) take poly-zwitter-ion liquid/polypyrrole/graphene oxide (PVIPS/PPy/GO) nanometer sheet, ultrasonic disperse, in absolute ethyl alcohol, obtains finely dispersed composite modified dose;
(4) clean glass-carbon electrode (GCE) surface is applied to by finely dispersed composite modified dose, dry under room temperature, obtain the glass-carbon electrode that poly-zwitter-ion liquid/polypyrrole/graphene oxide (PVIPS/PPy/GO) is modified.
Described preparation method, the mass ratio of step (2) described 1-vinyl-3-propane sulfonic acid base imidazole salts (VIPS) and N-vinyl imidazole type ionic liquid/polypyrrole/graphene oxide (ILs/PPy/GO) nanometer sheet is 5:1.
Described preparation method, step (2) ultrasonic disperse duration is 5 ~ 10min.
Described preparation method, the temperature of step (2) oil bath pan is 65 DEG C-75 DEG C, and condensing reflux reaction duration is 3 ~ 6h.
Described preparation method, in step (2), N-vinyl imidazole type ionic liquid/polypyrrole/graphene oxide (ILs/PPy/GO) nanometer sheet is polypyrrole/graphene oxide (PPy/GO) nanometer sheet of N-vinyl imidazole type Ionic Liquid Modified, and concrete preparation method is as follows:
Substitution reaction is there is and obtains PPy/GO-(CH in the polypyrrole/graphene oxide (PPy/GO) a. prepared by Isosorbide-5-Nitrae-dibromobutane and step (1) in DMF
2)
4br nanometer sheet;
B. by N-vinyl imidazole and PPy/GO-(CH
2)
4polypyrrole/graphene oxide (PPy/GO) nanometer sheet that ionization reaction obtains N-vinyl imidazole type Ionic Liquid Modified is there is in Br nanometer sheet in DMF.
Described preparation method, in step (2), 1-vinyl-3-propane sulfonic acid base imidazole salts (VIPS) is obtained by N-vinyl imidazole and PS ionization reaction.
A kind of modified electrode detecting low concentration dopamine is quantitatively detecting the application on dopamine.
Described application, using detecting the modified electrode of low concentration dopamine as working electrode, with Ag/AgCl electrode for contrast electrode, take platinum electrode as auxiliary electrode, composition three-electrode system is the detection realized in the phosphate buffer solution of 7.0 low concentration dopamine at pH.
The present invention compared with prior art, has following remarkable advantage:
Modified electrode prepared by the present invention, due to poly-zwitter-ion liquid (PVIPS) skeleton end-SO
3 -existence, change the charge property of material surface, PVIPS/PPy/GO nanometer sheet is made to show stronger negative electricity character, the DA molecule of positive electricity aobvious in solution can be enriched in working electrode surface, optimize electric transmission pattern, namely optimize the electronic transmission process of catalytic oxidation DA molecule, under the condition close to human body fluid potential of hydrogen, achieve the detection to super low concentration DA.
Modified electrode prepared by the present invention, shows the advantages such as higher selectivity, preferably stability, stronger interference free performance and the wider range of linearity.
Modified electrode prepared by the present invention makes the detection of nanomolar concentration DA be achieved, for the concentration accurately detecting DA molecule in actual sample provides new thinking and countermeasure.
Accompanying drawing explanation
Fig. 1 is the Electronic Speculum figure of PVIPS/PPy/GO nanometer sheet: (a) PVIPS/PPy/GO nanometer sheet scanning electron microscope (SEM) picture; (b) PVIPS/PPy/GO nanometer sheet transmission electron microscope (TEM) picture.
Fig. 2 is the cyclic voltammetric comparison diagram of different modifying electrode detection DA.
Fig. 3 is the cyclic voltammogram that PVIPS/PPy/GO modified electrode detects DA under different scanning speed condition.
Fig. 4 is the linear relationship of electric current and sweep velocity.
Fig. 5 is the differential pulse voltammetry figure that PVIPS/PPy/GO modified electrode detects variable concentrations DA.
Fig. 6 is electric current and DA concentration change linear relationship chart.
Fig. 7 is the test of PVIPS/PPy/GO modified electrode interference free performance.
Embodiment
A kind of modified electrode detecting low concentration dopamine, described modified electrode is the glass-carbon electrode that poly-zwitter-ion liquid/polypyrrole/graphene oxide (PVIPS/PPy/GO) is modified, being specially with glass-carbon electrode is basal electrode, polypyrrole/graphene oxide (PPy/GO) nanometer sheet that the poly-zwitter-ion liquid of attachment is modified on glass-carbon electrode.Poly-zwitter-ion liquid is poly-1-vinyl-3-propyl sulfonic acid imidazole salts (VIPS).
Detect a preparation method for the modified electrode of low concentration dopamine, comprise the steps:
(1) preparation of polypyrrole/graphene oxide (PPy/GO) nanometer sheet
Under the condition of ultrasonic radiation, the polymerization of pyrroles (Py) in-situ chemical is, in graphene oxide (GO) nanometer sheet that Hummers legal system is standby, to obtain polypyrrole/graphene oxide (PPy/GO) nanometer sheet;
(2) preparation of poly-zwitter-ion liquid/polypyrrole/graphene oxide (PVIPS/PPy/GO) nanometer sheet
Substitution reaction is there is and obtains PPy/GO-(CH in the polypyrrole/graphene oxide (PPy/GO) a. prepared by Isosorbide-5-Nitrae-dibromobutane and step (1) in DMF
2)
4br nanometer sheet;
B. by N-vinyl imidazole and PPy/GO-(CH
2)
4there is polypyrrole/graphene oxide (PPy/GO) nanometer sheet that ionization reaction obtains N-vinyl imidazole type Ionic Liquid Modified in Br nanometer sheet, i.e. N-vinyl imidazole type ionic liquid/polypyrrole/graphene oxide (ILs/PPy/GO) nanometer sheet in DMF;
C. N-vinyl imidazole and PS ionization reaction is utilized to obtain 1-vinyl-3-propane sulfonic acid base imidazole salts (VIPS);
D. 1-vinyl-3-propane sulfonic acid base imidazole salts (VIPS) and azoisobutyronitrile (AIBN) are scattered in ethanol, N-vinyl imidazole type ionic liquid/polypyrrole/graphene oxide (ILs/PPy/GO) nanometer sheet (mass ratio of VIPS and ILs/PPy/GO nanometer sheet is 5:1) of preparation is added again in system, ultrasonic disperse 5 ~ 10min, obtains potpourri; Potpourri is placed in the oil bath pan of 65 DEG C-75 DEG C, condensing reflux reaction 3 ~ 6h under nitrogen sustainable protection; After reaction terminates, with distilled water and ethanol, condensing reflux reaction product is repeatedly washed, centrifugal; By centrifugal rear products therefrom vacuum drying 24h, finally obtain black and gather zwitter-ion liquid/polypyrrole/graphene oxide (PVIPS/PPy/GO) nanometer sheet;
(3) take poly-zwitter-ion liquid/polypyrrole/graphene oxide (PVIPS/PPy/GO) nanometer sheet, ultrasonic disperse, in absolute ethyl alcohol, obtains finely dispersed composite modified dose;
(4) clean glass-carbon electrode (GCE) surface is applied to by finely dispersed composite modified dose, dry under room temperature, obtain the glass-carbon electrode that poly-zwitter-ion liquid/polypyrrole/graphene oxide (PVIPS/PPy/GO) is modified.
A kind of modified electrode detecting low concentration dopamine is quantitatively detecting the application on dopamine, to the modified electrode of low concentration dopamine be detected as working electrode, with Ag/AgCl electrode for contrast electrode, take platinum electrode as auxiliary electrode, composition three-electrode system is the detection realized in the phosphate buffer solution of 7.0 low concentration dopamine at pH.
Embodiment
(1) instrument used and reagent is tested:
The water used in experimentation is redistilled water (abbreviation intermediate water), test reagent used be analyze pure.
Instrument: model is that CHI660E electrochemical analyser (Shanghai Chen Hua instrument company) is for electrochemistry experiment; BT224S electronic balance (German Sartorius company) is for weighing medicine; JEM-2100 type transmission electron microscope (HIT) and HitachiSU-8010 type scanning electron microscope (HIT) are for morphology characterization; KH3200B ultrasonic cleaner (Kunshan Ultrasonic Instruments Co., Ltd.); Alundum (Al2O3) polishing powder (Shanghai Chen Hua instrument reagent company) is for the treatment of glass-carbon electrode (GCE); Silver/silver chloride electrode (Ag/AgCl, Wuhan Grolsch Rui Lian Science and Technology Ltd.) is contrast electrode; Platinum electrode is auxiliary electrode.
Reagent: high purity graphite, pyrroles, bromic ether, 1, 3-propane sultone (Chemical Reagent Co., Ltd., Sinopharm Group), N-vinyl imidazole (Yancheng City medication chemistry factory), sodium nitrate, potassium permanganate (Bo Di chemical inc, Tianjin), dopamine (DA) (AlfaAesar (China) Chemical Co., Ltd.), L-tryptophan (Trp), azoisobutyronitrile, Hydroxytryptamine hydrochloride (SER), adrenaline (Ep) (Aladdin reagent (Shanghai) Co., Ltd.), ascorbic acid (AA), phosphoric acid, sodium hydrogen phosphate, sodium dihydrogen phosphate (Tianjin Yong great chemical reagent company limited), (purity is 99.999% to high pure nitrogen, O
2≤ 0.001%).
(2) preparation process:
(1) composite modified dose of preparation, gets the PVIPS/PPy/GO nanometer sheet of 1mg drying, adds 1mL ethanol, ultrasonic disperse 20min, obtains the black suspension (namely finely dispersed composite modified dose) that concentration is 1mg/mL, for subsequent use.
(2) Electrode treatment: by glass-carbon electrode first by alundum (Al2O3) suspension grinding process on polishing cloth of 0.3 μM, then intermediate water ultrasonic cleaning is used, on polishing cloth, minute surface is polished to again with the alundum (Al2O3) suspension of 0.05 μM, finally use ethanol, intermediate water ultrasonic cleaning, dry up with high pure nitrogen.
(3) prepared by the glass-carbon electrode that PVIPS/PPy/GO modifies: pipette finely dispersed composite modified dose of 2 μ L with microsyringe and drip the surface being coated onto the clean glass-carbon electrode of polishing, natural drying in atmosphere, obtain the glass-carbon electrode modified with PVIPS/PPy/GO, for subsequent use.
As shown in Figure 1, (a) is scanning electron microscope (SEM) picture to the Electronic Speculum figure of PVIPS/PPy/GO nanometer sheet; B () is transmission electron microscope (TEM) picture, as seen from the figure by after VIPS and the surperficial ILs/PPy/GO copolymerization with vinyl-functional, nanometer sheet surface presentation goes out fold and sheet texture, at surface observation to some nanometer roll structures, can illustrate that poly-zwitter-ion liquid VIPS successfully modifies in polypyrrole/stannic oxide/graphene nano sheet surface simultaneously.
(3) performance test
1, contrast experiment
With microsyringe, a certain amount of DA is accurately moved in the electrolytic cell containing 8mLPBS (pH=7), respectively with bare electrode (bare), GO modified glassy carbon electrode, PPy/GO modified glassy carbon electrode and PVIPS/PPy/GO modified electrode are as working electrode, Ag/AgCl electrode is contrast electrode, and platinum electrode is as auxiliary electrode; Test is carried out on CHI660E electrochemical workstation, and its attached computer software is made for the acquisition and processing of experimental data; In-0.2V ~ 0.8V potential range, carry out cyclic voltammetry, record stable cyclic voltammogram.
Result as shown in Figure 2, for the cyclic voltammetric comparison diagram of different modifying electrode detection DA, wherein a curve is on the basis being working electrode with bare electrode (bare), b curve is on the basis being working electrode with GO modified glassy carbon electrode, c curve is on the basis being working electrode with PPy/GO modified glassy carbon electrode, as seen from the figure, all not there is oxidation peak in these three working electrodes.And d curve is on the basis being working electrode with PVIPS/PPy/GO modified electrode, then can be observed an oxidation peak, contrast show that PVIPS/PPy/GO modified electrode has good electro catalytic activity to when detecting low concentration DA.
2, DA is in the research of modified electrode surface kinetics
With PVIPS/PPy/GO modified electrode for working electrode, Ag/AgCl electrode is contrast electrode, and platinum electrode is auxiliary electrode; Experiment is carried out on CHI660E electrochemical workstation, comprises the acquisition and processing of experimental data; In the PBS solution (pH=7.0,20 DEG C) containing 200nMDA, carry out cyclic voltammetry scan in-0.2 ~ 0.8V potential range, sweep velocity scope is 10 ~ 100mV/s.
Be illustrated in figure 3 the cyclic voltammogram that PVIPS/PPy/GO modified electrode detects DA under different scanning speed condition.As can be seen from the figure, the oxidation peak current of DA increases along with the increase of sweep velocity.By research sweep speed on the impact of oxidation peak current, we can infer the dynamics of electrode reaction.
Be illustrated in figure 4 the linear relationship of PVIPS/PPy/GO modified electrode electric current and sweep velocity.As shown in the figure, sweep speed is within the scope of 10 ~ 100mV/s, and the oxidation peak current of DA becomes good linear relationship (R with sweep velocity
2=0.9968).It can thus be appreciated that under this experiment condition, the oxidizing process of DA is carried out in granule surface contral mode.This is because under the condition of pH=7.0, DA molecule (pKa=8.9) exists with cationic form, and PVEIB/PPy/GO nanometer sheet is due to poly-zwitter-ion liquid PVIPS skeleton end-SO
3 -existence show stronger electronegativity.This feature makes DA molecule in solution under the effect of electrostatic attraction, forms enrichment, thus greatly accelerates the speed of DA molecular diffusion to electrode surface, facilitate the catalytic oxidation process of DA at working electrode surface.
3, the quantitative measurment of DA:
With PVIPS/PPy/GO modified electrode for working electrode, Ag/AgCl electrode is contrast electrode, and platinum electrode is auxiliary electrode; Experiment is carried out on CHI660E electrochemical workstation, comprises the acquisition and processing of experimental data; In the PBS solution (pH=7.0,20 DEG C) containing different solubility DA (40 ~ 1220nm), carry out differential pulse scanning in-0.2 ~ 0.8V potential range, record stable differential pulse voltammogram.
Fig. 5 is the differential pulse voltammetry figure that PVIPS/PPy/GO modified electrode detects variable concentrations DA.As can be seen from the figure, along with the increase of DA concentration, its oxidation peak current is also increasing gradually.
Fig. 6 is electric current and DA concentration change linear relationship chart.As seen from the figure, in the scope of 40 – 1220nM, the concentration of DA and oxidation peak current have good linear relationship (R
2=0.9996), slope is-0.259 μ A/ μM.Be 3 (S/N=3) according to standard signal to noise ratio (S/N ratio), PVIPS/PPy/GO modified electrode detecting when detecting DA is limited to 17.3nM (n=10), and sensitivity is 0.259 μ A/ μM, and its performance is better than many existing DA detecting devices.
4, anti-interference test:
With PVIPS/PPy/GO modified electrode for working electrode, Ag/AgCl electrode is contrast electrode, and platinum electrode is auxiliary electrode; Experiment is carried out on CHI660E electrochemical workstation, comprises the acquisition and processing of experimental data.
The interference free performance of PVIPS/PPy/GO modified electrode is measured by galvanometry (i-t).Arranging and detecting voltage is 0.20V, under the condition of Keep agitation, 2 μ L0.8mMDA, 4 μ L20mMAA, 4 μ L20mMEp, 4 μ L20mMTrp and 4 μ L20mMSER are progressively added in 8mL buffer solution, be spaced apart 100s, in solution the concentration of each material be respectively 200nM, 10 μMs, 10 μMs, 10 μMs and 10 μMs.
Fig. 7 is the test of PVIPS/PPy/GO modified electrode interference free performance.When adding 2 μ L0.8mMDA in solution, occur stronger response current, the response time is approximately 13s, and when adding other material, does not occur obvious response current.
Claims (10)
1. one kind is detected the modified electrode of low concentration dopamine, it is characterized in that, described modified electrode is the glass-carbon electrode that poly-zwitter-ion liquid/polypyrrole/graphene oxide (PVIPS/PPy/GO) is modified, being specially with glass-carbon electrode is basal electrode, polypyrrole/graphene oxide (PPy/GO) nanometer sheet that the poly-zwitter-ion liquid of attachment is modified on glass-carbon electrode.
2. a kind of modified electrode detecting low concentration dopamine according to claim 1, is characterized in that, described poly-zwitter-ion liquid is poly-1-vinyl-3-propyl sulfonic acid imidazole salts (VIPS).
3. detect a preparation method for the modified electrode of low concentration dopamine, it is characterized in that, comprise the steps:
(1) preparation of polypyrrole/graphene oxide (PPy/GO) nanometer sheet
Under the condition of ultrasonic radiation, the polymerization of pyrroles's in-situ chemical is, on the stannic oxide/graphene nano sheet that Hummers legal system is standby, to obtain polypyrrole/graphene oxide (PPy/GO) nanometer sheet;
(2) preparation of poly-zwitter-ion liquid/polypyrrole/graphene oxide (PVIPS/PPy/GO) nanometer sheet
1-vinyl-3-propane sulfonic acid base imidazole salts (VIPS) and azoisobutyronitrile (AIBN) are scattered in ethanol, N-vinyl imidazole type ionic liquid/polypyrrole/graphene oxide (ILs/PPy/GO) nanometer sheet is added again in system, ultrasonic disperse, obtains potpourri;
By potpourri in oil bath pan, condensing reflux reaction under nitrogen sustainable protection;
After reaction terminates, with distilled water and ethanol, condensing reflux reaction product is repeatedly washed, centrifugal, by centrifugal rear products therefrom vacuum drying 24h, finally obtain black and gather zwitter-ion liquid/polypyrrole/graphene oxide (PVIPS/PPy/GO) nanometer sheet;
(3) take poly-zwitter-ion liquid/polypyrrole/graphene oxide (PVIPS/PPy/GO) nanometer sheet, ultrasonic disperse, in absolute ethyl alcohol, obtains finely dispersed composite modified dose;
(4) clean glass-carbon electrode (GCE) surface is applied to by finely dispersed composite modified dose, dry under room temperature, obtain the glass-carbon electrode that poly-zwitter-ion liquid/polypyrrole/graphene oxide (PVIPS/PPy/GO) is modified.
4. preparation method according to claim 3, it is characterized in that, the mass ratio of step (2) described 1-vinyl-3-propane sulfonic acid base imidazole salts (VIPS) and N-vinyl imidazole type ionic liquid/polypyrrole/graphene oxide (ILs/PPy/GO) nanometer sheet is 5:1.
5. preparation method according to claim 3, is characterized in that, step (2) ultrasonic disperse duration is 5 ~ 10min.
6. preparation method according to claim 3, is characterized in that, the temperature of step (2) oil bath pan is 65 DEG C-75 DEG C, and condensing reflux reaction duration is 3 ~ 6h.
7. preparation method according to claim 3, it is characterized in that, in step (2), N-vinyl imidazole type ionic liquid/polypyrrole/graphene oxide (ILs/PPy/GO) nanometer sheet is polypyrrole/graphene oxide (PPy/GO) nanometer sheet of N-vinyl imidazole type Ionic Liquid Modified, and concrete preparation method is as follows:
Substitution reaction is there is and obtains PPy/GO-(CH in the polypyrrole/graphene oxide (PPy/GO) a. prepared by Isosorbide-5-Nitrae-dibromobutane and step (1) in DMF
2)
4br nanometer sheet;
B. by N-vinyl imidazole and PPy/GO-(CH
2)
4polypyrrole/graphene oxide (PPy/GO) nanometer sheet that ionization reaction obtains N-vinyl imidazole type Ionic Liquid Modified is there is in Br nanometer sheet in DMF.
8. preparation method according to claim 3, is characterized in that, in step (2), 1-vinyl-3-propane sulfonic acid base imidazole salts (VIPS) is obtained by N-vinyl imidazole and PS ionization reaction.
9. the modified electrode detecting low concentration dopamine is quantitatively detecting the application on dopamine.
10. application according to claim 9, it is characterized in that, to the modified electrode of low concentration dopamine be detected as working electrode, with Ag/AgCl electrode for contrast electrode, take platinum electrode as auxiliary electrode, composition three-electrode system is the detection realized in the phosphate buffer solution of 7.0 low concentration dopamine at pH.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610005822.8A CN105548313A (en) | 2016-01-06 | 2016-01-06 | Modified electrode for detecting low-concentration dopamine and making method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610005822.8A CN105548313A (en) | 2016-01-06 | 2016-01-06 | Modified electrode for detecting low-concentration dopamine and making method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105548313A true CN105548313A (en) | 2016-05-04 |
Family
ID=55827646
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610005822.8A Pending CN105548313A (en) | 2016-01-06 | 2016-01-06 | Modified electrode for detecting low-concentration dopamine and making method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105548313A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106268961A (en) * | 2016-10-17 | 2017-01-04 | 辽宁大学 | The preparation method and applications of Ag/PAM/PPy/GO composite |
| CN106556633A (en) * | 2016-11-10 | 2017-04-05 | 无锡市明盛强力风机有限公司 | A kind of method of modifying of glass-carbon electrode |
| CN107312131A (en) * | 2017-07-12 | 2017-11-03 | 辽宁大学 | Polypyrrole/graphene oxide composite material of polymeric ionic liquid modification containing phenyl boric acid and its preparation method and application |
| WO2023004873A1 (en) * | 2021-07-27 | 2023-02-02 | 光华临港工程应用技术研发(上海)有限公司 | Dopamine detection apparatus and manufacturing method for dopamine detection electrode |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101576525A (en) * | 2009-06-10 | 2009-11-11 | 南京工业大学 | Method for preparing polymer modified electrode for selectively measuring dopamine |
| CN101710095A (en) * | 2009-11-10 | 2010-05-19 | 桂林理工大学 | Method for determining dopamine by utilizing charge recognition effect |
| CN102712779A (en) * | 2009-12-22 | 2012-10-03 | 徐光锡 | Graphene dispersion and graphene-ionic liquid polymer compound material |
| CN103969318A (en) * | 2014-04-29 | 2014-08-06 | 浙江大学 | Novel carbon nano tube electrochemical sensing interface and preparation method thereof |
-
2016
- 2016-01-06 CN CN201610005822.8A patent/CN105548313A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101576525A (en) * | 2009-06-10 | 2009-11-11 | 南京工业大学 | Method for preparing polymer modified electrode for selectively measuring dopamine |
| CN101710095A (en) * | 2009-11-10 | 2010-05-19 | 桂林理工大学 | Method for determining dopamine by utilizing charge recognition effect |
| CN102712779A (en) * | 2009-12-22 | 2012-10-03 | 徐光锡 | Graphene dispersion and graphene-ionic liquid polymer compound material |
| CN103969318A (en) * | 2014-04-29 | 2014-08-06 | 浙江大学 | Novel carbon nano tube electrochemical sensing interface and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| HUI MAO 等: "Poly(ionic liquids) functionalized polypyrrole/graphene oxide nanosheets for electrochemical sensor to detect dopamine in the presence of ascorbic acid", 《BIOSENSORS AND BIOELECTRONICS》 * |
| MASAHIRO YOSHIZAWA 等: "Design of ionic liquids for electrochemical applications", 《AUSTRALIAN JOURNAL OF CHEMISTRY》 * |
| REBECA MARCILLA 等: "Tuning the Solubility of Polymerized Ionic Liquids by Simple Anion-Exchange Reactions", 《JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY》 * |
| YI LI 等: "Electrochemical behavior of glassy carbon electrodes modified by multi-walled carbon nanotube/surfactant films in a buffer solution and an ionic liquid", 《CARBON》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106268961A (en) * | 2016-10-17 | 2017-01-04 | 辽宁大学 | The preparation method and applications of Ag/PAM/PPy/GO composite |
| CN106556633A (en) * | 2016-11-10 | 2017-04-05 | 无锡市明盛强力风机有限公司 | A kind of method of modifying of glass-carbon electrode |
| CN107312131A (en) * | 2017-07-12 | 2017-11-03 | 辽宁大学 | Polypyrrole/graphene oxide composite material of polymeric ionic liquid modification containing phenyl boric acid and its preparation method and application |
| CN107312131B (en) * | 2017-07-12 | 2019-07-02 | 辽宁大学 | Polypyrrole/graphene oxide composite material and its preparation method and application of polymeric ionic liquid modification containing phenyl boric acid |
| WO2023004873A1 (en) * | 2021-07-27 | 2023-02-02 | 光华临港工程应用技术研发(上海)有限公司 | Dopamine detection apparatus and manufacturing method for dopamine detection electrode |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103913492B (en) | A kind of Keggin-type heteropoly acid-polypyrrole-graphene composite material modified electrode and its preparation method and application | |
| Zhao et al. | Poly (isonicotinic acid) modified glassy carbon electrode for electrochemical detection of norepinephrine | |
| Zhu et al. | Single-walled carbon nanotubes modified carbon ionic liquid electrode for sensitive electrochemical detection of rutin | |
| CN103954673A (en) | Method for applying ionic liquid functionalized graphene modified electrode in detection of 5-hydroxytryptamine and dopamine | |
| Ensafi et al. | N-hexyl-3-methylimidazolium hexafluoro phosphate/multiwall carbon nanotubes paste electrode as a biosensor for voltammetric detection of morphine | |
| CN103604849B (en) | Electrochemical sensor capable of simultaneously detecting dopamine, ascorbic acid and uric acid | |
| CN105548313A (en) | Modified electrode for detecting low-concentration dopamine and making method and application thereof | |
| Kun et al. | Electrochemical behavior of propranolol hydrochloride in neutral solution on platinum nanoparticles doped multi-walled carbon nanotubes modified glassy carbon electrode | |
| CN102507697A (en) | Modified electrode based on molecular imprinted polymer membrane, electrochemistry system and application thereof | |
| CN103323516A (en) | Method for preparing non-enzyme H2O2 sensor based on ionic liquid functionalized carbon nanotube and silver nanometer compound | |
| CN105954336B (en) | A kind of no enzyme superoxide anion electrochemical sensor and its preparation method and application | |
| Javanbakht et al. | A selective and sensitive voltammetric sensor based on molecularly imprinted polymer for the determination of dipyridamole in pharmaceuticals and biological fluids | |
| Cheemalapati et al. | Highly sensitive and selective determination of pyrazinamide at poly-l-methionine/reduced graphene oxide modified electrode by differential pulse voltammetry in human blood plasma and urine samples | |
| CN102507683A (en) | Modified electrode based on functionalized multi-walled carbon nanotube, electrochemical system and application thereof | |
| Ghalkhani et al. | Electrochemical and DFT study of an anticancer and active anthelmintic drug at carbon nanostructured modified electrode | |
| CN105806924B (en) | A kind of 8-OHdG sensor and its preparation method and application | |
| Babaei et al. | A sensor for simultaneous determination of acetaminophen and codeine at glassy carbon electrode modified with multi-walled carbon nanotubes | |
| Desai et al. | Determination of amiloride at Nafion–CNT-nano-composite film sensor employing adsorptive stripping differential pulse voltammetry | |
| Yang | Voltammetric determination of tinidazole using a glassy carbon electrode modified with single-wall carbon nanotubes | |
| Lin et al. | Monolayer modification of glassy carbon electrode by using propionylcholine for selective detection of uric acid | |
| Atta et al. | Smart electrochemical morphine sensor using poly (3, 4-ethylene-dioxythiophene)/gold-nanoparticles composite in presence of surfactant | |
| CN101576530B (en) | Method for measuring dopamine by utilizing graphite nano-sheet/Nafion composite film to modify electrode | |
| Tian et al. | Electrodes with extremely high hydrogen overvoltages as substrate electrodes for stripping analysis based on bismuth-coated electrodes | |
| CN105572200A (en) | Modified glass-carbon electrode for detecting dopamine in presence of ascorbic acid, preparation method and applications thereof | |
| CN103913495B (en) | A kind of preparation method detecting the electrode of dopamine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160504 |