CN105548058B - A kind of method for detecting signaling molecule in pseudomonas aeruginosa - Google Patents
A kind of method for detecting signaling molecule in pseudomonas aeruginosa Download PDFInfo
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- 230000011664 signaling Effects 0.000 title claims abstract description 95
- 241000589517 Pseudomonas aeruginosa Species 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 23
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 claims abstract description 21
- 238000001514 detection method Methods 0.000 claims abstract description 20
- 241000589516 Pseudomonas Species 0.000 claims abstract description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 30
- 238000010521 absorption reaction Methods 0.000 claims description 18
- 238000000862 absorption spectrum Methods 0.000 claims description 18
- 239000010949 copper Substances 0.000 claims description 14
- 239000012046 mixed solvent Substances 0.000 claims description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 8
- 239000002904 solvent Substances 0.000 claims description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 6
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- 229910021591 Copper(I) chloride Inorganic materials 0.000 claims description 3
- 229910052802 copper Inorganic materials 0.000 claims description 3
- OXBLHERUFWYNTN-UHFFFAOYSA-M copper(I) chloride Chemical compound [Cu]Cl OXBLHERUFWYNTN-UHFFFAOYSA-M 0.000 claims description 3
- NWFNSTOSIVLCJA-UHFFFAOYSA-L copper;diacetate;hydrate Chemical compound O.[Cu+2].CC([O-])=O.CC([O-])=O NWFNSTOSIVLCJA-UHFFFAOYSA-L 0.000 claims description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims 2
- 238000007689 inspection Methods 0.000 claims 1
- 238000004847 absorption spectroscopy Methods 0.000 abstract 2
- 229910021645 metal ion Inorganic materials 0.000 description 6
- 229910021592 Copper(II) chloride Inorganic materials 0.000 description 3
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000007923 virulence factor Effects 0.000 description 3
- 239000000304 virulence factor Substances 0.000 description 3
- PHSRRHGYXQCRPU-AWEZNQCLSA-N N-(3-oxododecanoyl)-L-homoserine lactone Chemical compound CCCCCCCCCC(=O)CC(=O)N[C@H]1CCOC1=O PHSRRHGYXQCRPU-AWEZNQCLSA-N 0.000 description 2
- YKYOUMDCQGMQQO-UHFFFAOYSA-L cadmium dichloride Chemical compound Cl[Cd]Cl YKYOUMDCQGMQQO-UHFFFAOYSA-L 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 206010011409 Cross infection Diseases 0.000 description 1
- QJPWUUJVYOJNMH-VKHMYHEASA-N L-homoserine lactone Chemical compound N[C@H]1CCOC1=O QJPWUUJVYOJNMH-VKHMYHEASA-N 0.000 description 1
- 201000009906 Meningitis Diseases 0.000 description 1
- 229910021586 Nickel(II) chloride Inorganic materials 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 229910001431 copper ion Inorganic materials 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000018612 quorum sensing Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 208000013223 septicemia Diseases 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000011592 zinc chloride Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of method for detecting signaling molecule in pseudomonas aeruginosa.The result of study of ultraviolet-visible absorption spectroscopy shows, signaling molecule and Cu in pseudomonas aeruginosa2+There is no absworption peak, signaling molecule and Cu in 280nm or so2+With reference to rear, the new absworption peak of appearance at 280nm or so places, and good linear relationship is presented in the intensity of absworption peak and the concentration of signaling molecule.Advantages of the present invention：The method of the present invention is easy to operate, cost is low, by ultraviolet-visible absorption spectroscopy passage, utilizes signaling molecule in pseudomonas aeruginosa and Cu2+Coordination, realize the quantitative detection to signaling molecule in pseudomonas aeruginosa in different solutions.
Description
Technical field
The invention belongs to chemical analysis test technical field, and in particular to a kind of to detect signaling molecule in pseudomonas aeruginosa
Method, it is a kind of new detection method.
Background technology
Pseudomonas aeruginosa (Pseudomonase aeruginosa, PA), belong to gram-Negative bacillus, water, air,
Widely distributed in the skin of soil and living organism, respiratory tract and enteron aisle etc., according to investigations, which is the main of inside-hospital infection
One of pathogen, can trigger skin disease, pneumonia, intestines problem, meningitis and septicemia etc. after infection, can cause when serious dead
Die.Research shows, the signaling molecule N- (3- of pseudomonas aeruginosa population effect (quorum sensing, QS) system
oxododecanoyl)homoserine lactone(3OC12- HSL) in regulation and control pseudomonas aeruginosa overwhelming majority virulence factor
Expression in play an important role, and pseudomonas aeruginosa produce various severe infections and its virulence factor the close phase of release
Close.When pseudomonas aeruginosa number is relatively low, only produce the signaling molecule of foundation level, with pseudomonas aeruginosa it is local not
Medium well is grown, and signaling molecule can reach certain concentration in local environment, when its concentration reaches threshold values, just activate P. aeruginosa
The expression of related virulence factor, strengthens the pathogenic of pseudomonas aeruginosa, causes life entity to infect in bacterium.Therefore, exploitation is quick
It is of great significance with the method for sensitive accurate detection pseudomonas aeruginosa to the health of life entity.
At present, pseudomonas aeruginosa context of detection, it is necessary to multiple steps such as pure culture and biochemical identification, operation is numerous
It is trivial, detection cycle is long, sensitivity is low, and cannot meet the needs of quickly detecting.The concentration of signaling molecule and pseudomonas aeruginosa
Number it is related, by the measure to signaling molecule concentration in pseudomonas aeruginosa, monitoring pseudomonas aeruginosa life can be reached
The purpose of long quantity.Signaling molecule has multiple metal ion binding sites (such as O, N) in pseudomonas aeruginosa, according to signal point
The combination of son and metal ion, so that the research for detecting signaling molecule in pseudomonas aeruginosa has not been reported.
Based on the above problem, this invention address that signal in easy, the quick and sensitive detection pseudomonas aeruginosa of exploitation
The method of molecule.By uv-visible absorption spectra, using copper ion solution, realize to signaling molecule in pseudomonas aeruginosa
Quantitative detection.Not only method is easy by the present invention, and has protrusion in sensitivity, response time and biologic applications etc.
Advantage.
The content of the invention
The object of the present invention is to provide a kind of method for detecting signaling molecule in pseudomonas aeruginosa.
Technical scheme is as follows：
1. signaling molecule N- (3-oxododecanoyl) homoserine lactone in one kind detection pseudomonas aeruginosa
(3OC12- HSL) new method, signaling molecule and Cu in pseudomonas aeruginosa2+After coordination combines, there is following structural formula：
2. a kind of method for detecting signaling molecule in pseudomonas aeruginosa, comprises the following steps：
In DMSO and H2O volume ratios are 4:1 in the mixed solvent, adds the CuCl that initial concentration is 1.0mM2·2H2O,
Then, signaling molecule in the pseudomonas aeruginosa that different amounts of initial concentration is 1.0mM is sequentially added so that Cu in solution2+'s
Concentration is 5.0x10-4M, in pseudomonas aeruginosa the concentration of signaling molecule be respectively 1.0 μM, 2.0 μM, 5.0 μM, 10.0 μM,
20.0 μM, 50.0 μM, 100 μM, 200 μM, 300 μM, 400 μM, 500 μM, signaling molecule is added without as control, stands 30min
System reaches balance, and the purple under the conditions of various concentrations pseudomonas aeruginosa signaling molecule is tested with uv-visible absorption spectra instrument
Outside-visible absorption spectra, is examined in a manner of absorption peak strength at 277nm in uv-visible absorption spectra and 280nm is substantially change
Survey signaling molecule in pseudomonas aeruginosa；The DMSO and H2The pH value of O mixed solvents is by being added dropwise hydrochloric acid and sodium hydroxide tune
Save the pH to 6.5 of detection architecture.
A kind of above-mentioned method for detecting signaling molecule in pseudomonas aeruginosa, applied to Cu2+CH3OH/H2Copper in O solution
The detection of signaling molecule in green pseudomonad, the side being substantially change with absorption peak strength at 277nm in uv-visible absorption spectra
Signaling molecule in formula detection pseudomonas aeruginosa.
A kind of above-mentioned method for detecting signaling molecule in pseudomonas aeruginosa, applied to Cu2+DMF/H2Verdigris in O solution
The detection of signaling molecule in pseudomonad, in a manner of absorption peak strength is substantially change at 280nm in uv-visible absorption spectra
Detect signaling molecule in pseudomonas aeruginosa.
In the present invention, above-mentioned Cu2+DMSO/H2O、CH3OH/H2O and DMF/H2It is false that detection verdigris can be quantified in O solution
Signaling molecule in monad, the Cu2+Solution there is no ultraviolet-ray visible absorbing peak at 280nm or so places in itself, when with signaling molecule
With reference to rear, significantly increased at the ultraviolet-ray visible absorbing peak at 280nm or so places.
Beneficial effects of the present invention：
1. the new method of signaling molecule, has in life science and analysis field in the detection pseudomonas aeruginosa of the present invention
Broad prospect of application.
2. the new method of the present invention is obvious to the detected artifacts of signaling molecule in pseudomonas aeruginosa, easy to P. aeruginosa
The quantitative judge of signaling molecule in bacterium.
3. the new method of signaling molecule is easy to operate in the detection pseudomonas aeruginosa of the present invention, quick, cost is low.
Brief description of the drawings
Fig. 1 be in the embodiment of the present invention 1 in pseudomonas aeruginosa signaling molecule different metal ions are responded it is ultraviolet-can
See abosrption spectrogram.
Fig. 2 is Cu in the embodiment of the present invention 22+With under the conditions of signaling molecule in various concentrations pseudomonas aeruginosa it is ultraviolet-
Visible absorption spectra figure.
In figure, solvent used is DMSO/H2O(4/1,v/v),Cu2+Concentration be 5.0 × 10-4M；Nethermost curve
To be added without signaling molecule 3OC12The absorption curve of-HSL, curve signaling molecule 3OC from the bottom up12The concentration of-HSL increases successively
Add.
Fig. 3 is the pass of signaling molecule concentration in absorption intensity and pseudomonas aeruginosa in the embodiment of the present invention 2 at 277nm
System's figure.
In figure, solvent used is DMSO/H2O(4/1,v/v),Cu2+Concentration be 5.0 × 10-4M。
Fig. 4 is Cu in the embodiment of the present invention 32+With under the conditions of signaling molecule in various concentrations pseudomonas aeruginosa it is ultraviolet-
Visible absorption spectra figure.
In figure, solvent used is CH3OH/H2O(4/1,v/v),Cu2+Concentration be 5.0 × 10-4M；Nethermost curve
To be added without signaling molecule 3OC12The absorption curve of-HSL, curve signaling molecule 3OC from the bottom up12The concentration of-HSL increases successively
Add.
Fig. 5 is the pass of signaling molecule concentration in absorption intensity and pseudomonas aeruginosa in the embodiment of the present invention 3 at 277nm
System's figure.
In figure, solvent used is CH3OH/H2O(4/1,v/v),Cu2+Concentration be 5.0 × 10-4M。
Fig. 6 is Cu in the embodiment of the present invention 42+With under the conditions of signaling molecule in various concentrations pseudomonas aeruginosa it is ultraviolet-
Visible absorption spectra figure.
In figure, solvent used is DMF/H2O(4/1,v/v),Cu2+Concentration be 5.0 × 10-4M；Nethermost curve is
It is added without signaling molecule 3OC12The absorption curve of-HSL, curve signaling molecule 3OC from the bottom up12The concentration of-HSL increases successively.
Fig. 7 is the pass of signaling molecule concentration in absorption intensity and pseudomonas aeruginosa in the embodiment of the present invention 4 at 280nm
System's figure.
In figure, solvent used is DMF/H2O(4/1,v/v),Cu2+Concentration be 5.0 × 10-4M。
Specific embodiment
Below by specific embodiment, the present invention will be further described, but the invention is not restricted to embodiment.
It is raw materials used in embodiment, it is conventional commercial products unless otherwise specified.
Signaling molecule N- (3-oxododecanoyl) homoserine lactone in one kind detection pseudomonas aeruginosa
(3OC12- HSL) method, signaling molecule and Cu in pseudomonas aeruginosa2+After coordination combines, there is following structural formula：
Embodiment 1
Response Journal of Sex Research of the signaling molecule to metal ion in pseudomonas aeruginosa
In DMSO and H2O volume ratios are 4:1 in the mixed solvent (pH=6.5), by the verdigris that initial concentration is 20 μM
Signaling molecule is 200 μM of CuCl with initial concentration respectively in pseudomonad2·2H2O、CdCl2、NiCl2And ZnCl2Mixing,
Configuration detection sample so that the concentration of signaling molecule is 10 μM in pseudomonas aeruginosa in sample, Cu2+、Cd2+、Ni2+And Zn2+'s
Concentration is 100 μM, is added without metal ion as control, stands 30min systems and reach balance.
Response condition with signaling molecule in uv-visible absorption spectra instrument test pseudomonas aeruginosa to metal ion,
The results are shown in Figure 1.As shown in Figure 1, Cd is added2+、Ni2+And Zn2+Afterwards, in pseudomonas aeruginosa signaling molecule ultraviolet-visible
Absorption spectrum does not change substantially；Only as addition Cu2+Afterwards, there is new absworption peak under 277nm wavelength, illustrate pseudomonas aeruginosa
Middle signaling molecule can be with Cu2+Coordination combines.
Embodiment 2
It is as follows that a kind of method for detecting signaling molecule in pseudomonas aeruginosa includes step：
In DMSO and H2O volume ratios are 4:1 in the mixed solvent (pH=6.5), it is 1.0mM's to add initial concentration
CuCl2·2H2O, then, sequentially adds signaling molecule in the pseudomonas aeruginosa that different amounts of initial concentration is 1.0mM so that
Cu in solution2+Concentration be 5.0x10-4M, the concentration of signaling molecule is respectively 1.0 μM, 2.0 μM, 5.0 μ in pseudomonas aeruginosa
M, 10.0 μM, 20.0 μM, 50.0 μM, 100 μM, 200 μM, 300 μM, 400 μM, 500 μM, it is added without signal in pseudomonas aeruginosa
Molecule stands 30min systems and reaches balance as control.
The ultraviolet-visible in different pseudomonas aeruginosas under the conditions of signaling molecule is tested with uv-visible absorption spectra instrument
Absorption spectrum, the results are shown in Figure 2.As shown in Figure 2, with the concentration increase of signaling molecule in pseudomonas aeruginosa, in 277nm
Absorption intensity under wavelength gradually strengthens.From the figure 3, it may be seen that signaling molecule in absorption intensity and pseudomonas aeruginosa at 277nm
Concentration there is good linear relationship, it was demonstrated that Cu2+DMSO/H2O solution, in 2.0 μM of -100 μM of concentration ranges, Ke Yiding
Signaling molecule in amount detection pseudomonas aeruginosa.
Embodiment 3
It is as follows that a kind of method for detecting signaling molecule in pseudomonas aeruginosa includes step：
In CH3OH and H2O volume ratios are 4:1 in the mixed solvent (pH=6.5), it is 1.0mM's to add initial concentration
CuCl2·2H2O, then, sequentially adds signaling molecule in the pseudomonas aeruginosa that different amounts of initial concentration is 1.0mM so that
Cu in solution2+Concentration be 5.0x10-4M, the concentration of signaling molecule is respectively 1.0 μM, 2.0 μM, 5.0 μ in pseudomonas aeruginosa
M, 10.0 μM, 20.0 μM, 50.0 μM, 100 μM, 200 μM, 300 μM, 400 μM, 500 μM, it is added without signal in pseudomonas aeruginosa
Molecule stands 30min systems and reaches balance as control.
The ultraviolet-ray visible absorbing in different pseudomonas aeruginosas under the conditions of signaling molecule is tested with ultraviolet-ray visible absorbing instrument
Spectrum, the results are shown in Figure 4.As shown in Figure 4, with the concentration increase of signaling molecule in pseudomonas aeruginosa, in 277nm wavelength
Under absorption intensity gradually strengthen.As shown in Figure 5, in the absorption intensity at 277nm and pseudomonas aeruginosa signaling molecule it is dense
Degree has good linear relationship, it was demonstrated that Cu2+CH3OH/H2O solution, in 100 μM -500 μM of concentration range, Ke Yiding
Signaling molecule in amount detection pseudomonas aeruginosa.
Embodiment 4
It is as follows that a kind of method for detecting signaling molecule in pseudomonas aeruginosa includes step：
In DMF and H2O volume ratios are 4:1 in the mixed solvent (pH=6.5), it is 1.0mM's to add initial concentration
CuCl2·2H2O, then, sequentially adds signaling molecule in the pseudomonas aeruginosa that different amounts of initial concentration is 1.0mM so that
Cu in solution2+Concentration be 5.0x10-4M, the concentration of signaling molecule is respectively 1.0 μM, 2.0 μM, 5.0 μ in pseudomonas aeruginosa
M, 10.0 μM, 20.0 μM, 50.0 μM, 100 μM, 200 μM, 500 μM, it is added without signaling molecule conduct pair in pseudomonas aeruginosa
According to standing 30min systems reach balance.
The ultraviolet-ray visible absorbing in different pseudomonas aeruginosas under the conditions of signaling molecule is tested with ultraviolet-ray visible absorbing instrument
Spectrum, the results are shown in Figure 6.It will be appreciated from fig. 6 that with the concentration increase of signaling molecule in pseudomonas aeruginosa, in 280nm wavelength
Under absorption intensity gradually strengthen.As shown in Figure 7, in the absorption intensity at 280nm and pseudomonas aeruginosa signaling molecule it is dense
Degree has good linear relationship, it was demonstrated that Cu2+DMF/H2O solution, in 1.0 μM -50 μM of concentration range, can quantify and examine
Survey signaling molecule in pseudomonas aeruginosa.
Claims (3)
- A kind of 1. method for detecting signaling molecule in pseudomonas aeruginosa, it is characterised in that comprise the following steps：In DMSO and H2O Volume ratio is 4:1 in the mixed solvent, adds the CuCl that initial concentration is 1.0mM2·2H2O, then, sequentially adds not same amount Initial concentration be 1.0mM pseudomonas aeruginosa in signaling molecule, obtain signaling molecule and Cu in pseudomonas aeruginosa2+Coordination Molecular structural formula with reference to after is：And cause Cu in solution2+Concentration be 5.0x10-4M, in pseudomonas aeruginosa the concentration of signaling molecule be respectively 1.0 μM, 2.0 μM, 5.0 μM, 10.0 μM, 20.0 μM, 50.0 μM, 100 μM, 200 μM, 300 μM, 400 μM, 500 μM, it is false single to be added without verdigris Signaling molecule stands 30min systems and reaches balance, tested with uv-visible absorption spectra instrument different dense as control in born of the same parents bacterium The uv-visible absorption spectra under the conditions of signaling molecule in pseudomonas aeruginosa is spent, passes through signaling molecule in pseudomonas aeruginosa With Cu2+Coordination detects copper with reference to the rear molecule mode that absorption peak strength is substantially change at 277nm in uv-visible absorption spectra Signaling molecule in green pseudomonad；The DMSO and H2The pH value of O mixed solvents adjusts inspection by the way that hydrochloric acid and sodium hydroxide is added dropwise The pH of survey system to 6.5.
- A kind of 2. method for detecting signaling molecule in pseudomonas aeruginosa as claimed in claim 1, it is characterised in that the mixing Solvent can also be CH3OH/H2O volume ratios are 4:1 mixed solvent；Applied to Cu2+CH3OH/H2Verdigris is false in O solution The detection of signaling molecule, is examined in a manner of absorption peak strength is substantially change at 277nm in uv-visible absorption spectra in monad Survey signaling molecule in pseudomonas aeruginosa.
- A kind of 3. method for detecting signaling molecule in pseudomonas aeruginosa as claimed in claim 1, it is characterised in that the mixing Solvent can also be DMF/H2O volume ratios are 4:1 mixed solvent；Applied to Cu2+DMF/H2P. aeruginosa in O solution The detection of signaling molecule, detects copper in a manner of absorption peak strength is substantially change at 280nm in uv-visible absorption spectra in bacterium Signaling molecule in green pseudomonad.
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