CN105547516B - Laser pump (ing) up-conversion fluorescence temp measuring system - Google Patents
Laser pump (ing) up-conversion fluorescence temp measuring system Download PDFInfo
- Publication number
- CN105547516B CN105547516B CN201610005174.6A CN201610005174A CN105547516B CN 105547516 B CN105547516 B CN 105547516B CN 201610005174 A CN201610005174 A CN 201610005174A CN 105547516 B CN105547516 B CN 105547516B
- Authority
- CN
- China
- Prior art keywords
- temperature
- fluorescence
- conversion
- semiconductor laser
- sample stage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K11/00—Measuring temperature based upon physical or chemical changes not covered by groups G01K3/00, G01K5/00, G01K7/00 or G01K9/00
Abstract
The invention belongs to optical devices technologies field, specially a kind of laser pump (ing) up-conversion fluorescence temp measuring system.The present invention is made of semiconductor laser, sample stage, loop temperature-control device, body phase thermo detector, optical filter, CCD detector and computer.The system can have to label the material, cell or biological tissue of up-conversion luminescence probe carry out in real time, the imaging of non-intrusion type material monitored, in cell or biological tissue the case where temperature change by the acquisition to up-conversion fluorescence；In addition, the system can the correlation of optical property and temperature-responsive to fluorescence temperature probe detected and realized the microcell heating of up-conversion fluorescence temperature probe, for measuring the temperature of the optical-thermal conversion material in conjunction with up-conversion fluorescence temperature probe.The operability and real-time of fluorescence temperature detection can be improved in the present invention, provides a kind of strong tool for material science, life science and medical research and the application of fluorescence temperature detection technique.
Description
Technical field
The invention belongs to optical devices technologies fields, and in particular to a kind of laser pump (ing) up-conversion fluorescence temp measuring system.
Background technique
Optical temperature sensing is of great significance in fields such as industry, military affairs, medical treatment.Different from other contacts
Temp measuring method such as thermally expand thermometric, thermal resistance thermometric, thermoelectricity thermometric, optics thermometric can be realized the contactless temperature to sample
Degree detection, sample such as cell, micro-nano device for some extra small scales and requires low damage or even lossless sample such as living body
For tissue, optics thermometric is a kind of thermometric mode that can not be substituted.It is swift and violent in recent decades in view of detection technique of fluorescence
Development, the sensitivity of fluorescence detection and accuracy are all greatly improved, therefore carry out optical temperature using fluorescence signal
Detecting has the advantages such as the sample size that detection speed is fast, needs is few, detection sensitivity is high.Recently, small molecule or nanometer are based on
The fluorescence temperature probe of material receives domestic and international researcher and more and more payes attention to, and is carried out by fluorescence temperature probe to sample
Label can be realized the real-time tracking of sample temperature variation, specificity, selectivity observation and high-resolution imaging etc..At present can
The probe for realizing fluorescence temperature detection includes temperature sensitive fluorescent small molecule, quantum dot, Nano diamond, fluorescent polymer nano material
With rare earth upconversion nano crystal etc., the working principle of these temperature sensitive probes is mainly the i on population and temperature of the emission level of fluorescence
The linear relevant relationship of index, that is, meet Bu Erziman distribution, pass through the detection changed to fluorescence signal intensity, so that it may
Know the temperature value of environment locating for Current Temperatures probe.These above-mentioned fluorescence temperature probes material science, life science and
Medical domain has a wide range of applications, than realizing micro-nano device using temperature sensitive fluorescent small molecule if any document report
Temperature detection, realize the heating monitoring of high-resolution cell using Nano diamond, realized using up-conversion luminescence nanometer crystal
The temperature detection etc. of photo-thermal therapy.As can be seen from these examples, fluorescence-based temperature detection technology will push significantly receives
Rice materialogy, life science, medicine develop towards more accurate direction.
It has been reported that fluorescence temperature probe in, the fluorescence temperature probe of the rare earth up-conversion luminescence based on laser pump (ing)
There are some unique properties and is better than other probes.Firstly, the mechanism of up-conversion luminescence and the mechanism of general fluorescence probe are not
It together, is the process for converting the near-infrared photon of two or more low energy to a high-energy photons.Such light emitting machine
Reason can substantially reduce the interference of background auto-fluorescence, pass through the laser excitation of near-infrared, additionally it is possible to improve the depth of detection simultaneously
And reduce damage of the exciting light to sample especially biological tissue.In addition, general fluorescence temperature probe is all single spectral band emissive,
The inaccuracy of detection can be caused because absorption, scattering or the position of sample are mobile.Rare earth up-conversion luminescence temperature probe
Another feature be more spectral band emissives, the energy level distribution relation of these more spectral band emissives meets Bu Erziman distribution, thus can be real
Existing fluorescence thus avoids the error of single bands of a spectrum detection than degree temperature detection.Such as the upper conversion nano material of rare earth Er ions
Two green emissive bands of material (respectively correspond energy level transition2H11/2→4I15/2With4S3/2→4I15/2) emissive porwer ratio and temperature
Degree is related, therefore is used as than spending temperature probe.Fluorescence temperature detection at present still lacks also in developing stage based on upper
The luminous fluorescence temperature detection system of conversion, if it is possible to by the up-conversion luminescence acquisition technique of laser pump (ing) and temperature detection skill
Art combines, and develops a kind of novel fluorescence temperature detection system, will efficiently contribute to improve grasping for fluorescence temperature detection
The property made and real-time, the application for material science, life science and medical research and fluorescence temperature detection technique provide one kind
Strong tool.
Summary of the invention
The purpose of the present invention is to provide a kind of up-conversion fluorescence system for detecting temperature of laser pump (ing), which can be right
The material, cell or biological tissue for being marked with temperature sensitive up-conversion luminescence probe carry out real-time, non-intrusion type imaging,
It can be by the acquisition to up-conversion fluorescence, in monitoring material, cell or biological tissue the case where temperature change.
Laser pump (ing) up-conversion fluorescence system for detecting temperature proposed by the present invention, structure composition is as shown in Figure 1, by first
Semiconductor laser 1, the second semiconductor laser 2, sample stage 3, loop temperature-control device 4, body phase thermo detector 5, optical filter 6, CCD inspection
It surveys device 7 and computer 8 forms；Wherein, sample stage 3 for place fluorescence temperature probe material, Tissue Culture Dish, living animal with
And tissue；Fluorescence excitaton source of first semiconductor laser 1 as fluorescence temperature probe, outgoing optical registration sample stage 3, second
Photic heating source of the semiconductor laser 2 as fluorescence temperature probe；It is directed at sample stage 3, loop temperature-control device 4 surrounds sample stage 3,
Body phase thermo detector 5 is connect by temperature probe with sample stage 3, and loop temperature-control device 4 and body phase thermo detector 5 are used to visit fluorescence temperature
The light intensity of needle is measured with temperature dependency curve；Place optical filter 6, the light-emitting window alignment of optical filter 6 in the front of sample stage 3
CCD detector 7, CCD detector 7 connect computer 8 by signal receiving circuit.
In the present invention, first semiconductor laser 1 can be used that central wavelength is 980 nm, 808 nm, 1532 nm are partly led
Body laser.
In the present invention, second semiconductor laser 2 can be used that central wavelength is 808 nm, 730 nm, 1064 nm are partly led
Body laser.
In the present invention, the short pass filter of 720 nm, 850 nm is can be used in optical filter 6.
The present invention can the correlation of optical property and temperature-responsive to fluorescence temperature probe detect, to laser controlling
Fluorescence temperature probe carry out microcell heating and can have material, cell and the living animal and group of probe to marking
It knits and carries out real time temperature change monitoring.
The course of work of the invention is: the laser beam that the first semiconductor laser 1 generates is radiated on sample stage 3, sample
The label carried on platform 3 has material, cell or the living animal of temperature probe and tissue can be in laser
Excitation under emit up-conversion luminescence, after a part of up-conversion luminescence signal passes through the signal for intercepting certain wavelength after optical filter 6
It is received by CCD detector 7, digitized processing is carried out by computer 8 by the received fluorescence signal of CCD detector 7 and obtains temperature
Value.
The present invention can also carry out fluorescence-temperature correction curve determination of fluorescence temperature probe, pass through loop temperature-control device 4
External heating, In are carried out to the material containing fluorescence temperature probe, cell or living animal and tissue with body phase thermo detector 5
Under the irradiation of semiconductor laser 1, the up-conversion fluorescence signal under different external heating temperatures is acquired, up-conversion luminescence signal is logical
It is received after crossing after optical filter 6 signal for intercepting certain wavelength by CCD detector 7, is led to by the received fluorescence signal of CCD detector 7
It crosses the progress of computer 8 digitized processing and obtains fluorescence-temperature correction curve.
The present invention can also carry out the heating of laser microcell to fluorescence temperature probe, using the second semiconductor laser 2 to containing
Material, cell or the living animal and tissue for having fluorescence temperature probe carry out microcell heating, in the first semiconductor laser 1
Irradiation under, acquire the up-conversion fluorescence signal under different external heating temperatures, up-conversion luminescence signal is cut after passing through optical filter 6
It is received after taking the signal of certain wavelength by CCD detector 7, is carried out by the received fluorescence signal of CCD detector 7 by computer 8
Digitized processing obtains the temperature variation curve of laser microcell heating.
The utility model has the advantages that compared with prior art, the invention has the following advantages that
1. monitoring than degree fluorescence temperature for up-conversion fluorescence temperature probe may be implemented in the present invention, sample can be eliminated
Autofluorescence, absorption, scattering or position are mobile and cause the inaccuracy of detection；
2. the measurement of up-conversion fluorescence temperature probe fluorescence-temperature correction curve may be implemented in the present invention, in same system
The middle measurement for realizing calibration curve and temperature detection can be to avoid inexactnesies caused by the systematic error difference of instrument；
3. the microcell heating of up-conversion fluorescence temperature probe may be implemented in the present invention, it can be used for measurement and up-conversion fluorescence
The temperature for the optical-thermal conversion material that temperature probe combines.This will efficiently contribute to the temperature-raising characteristic of research optical-thermal conversion material, and
Photo-thermal therapy to carry out at present provides more information and strategy.
Detailed description of the invention
Basic structure schematic diagram Fig. 1 of the invention.
Fluorescence-temperature correction curve of Fig. 2 embodiment 1.
The microcosmic monitoring temperature curve of Fig. 3 embodiment 2.
Specific embodiment
Example is used to further illustrate the present invention in detail below.
Embodiment 1: up-conversion fluorescence temperature probe is measured using laser pump (ing) up-conversion fluorescence temp measuring system
NaLu0.78Yb0.2Er0.02F4@NaLuF4Fluorescence-temperature correction curve
By the nano material NaLu of up-conversion fluorescence temperature probe0.78Yb0.2Er0.02F4@NaLuF4It is dispersed in water, prepares
At 2 mL of solution of 0.5 mg/mL, which is placed in cuvette, is placed on sample stage 3 as shown in Figure 1.Using following
Ring thermostat 4 and body phase thermo detector 5 carry out alternating temperature from 0 DEG C to 90 DEG C, while exciting up-conversion fluorescence, upper conversion using 980 nm
Luminous signal is received after the signal by intercepting certain wavelength after optical filter 6 by CCD detector 7, received by CCD detector 7
Fluorescence signal carries out the integral and ratio of fluorescence emission peak, the standard curve that fluorescent emission varies with temperature by computer 8.Such as
Shown in Fig. 2, fluorescence intensity as the temperature rises linear pertinent trends.
Embodiment 2: the upper conversion in conjunction with photo-thermal therapy material is measured using laser pump (ing) up-conversion fluorescence temp measuring system
Microcosmic monitoring temperature of the fluorescence temperature probe under photo-thermal excitation
By the up-conversion fluorescence temperature probe NaLu in conjunction with optical-thermal conversion material0.78Yb0.2Er0.02F4@NaLuF4Dispersion
Yu Shuizhong is configured to 2 mL of solution of 0.5 mg/mL.Use 300mW/cm2730 nm laser to solution carry out prolonged exposure,
Different time points are taken in 0-8 minutes, carry out solution thermometric using body phase thermo detector 5, using 980 nm laser excitations, in acquisition
Switching emission spectrum, up-conversion luminescence signal are received after the signal by intercepting certain wavelength after optical filter 6 by CCD detector 7,
By the received fluorescence signal of CCD detector 7 by computer 8, chooses two transmitting bands of 815 nm and 840 nm and emit by force
Degree integral and ratio, bring the standard curve that fluorescent emission varies with temperature into, obtain microcosmic temperature change result.As shown in figure 3,
It can observe the up-conversion fluorescence temperature probe NaLu that optical-thermal conversion material combines0.78Yb0.2Er0.02F4@NaLuF4It is microcosmic
Temperature rises to 67.5 DEG C, illustrates that optothermal material can rise to temperature more higher than macrostate under microcosmic.
Although detailing the present invention with preferred embodiment, it is not intended to limit the present invention.Any this field
Technical staff, without departing from the spirit and scope of the present invention, should can with various modification can be adapted with change.Therefore
Protection scope of the present invention should be considered as appended claims limited range.
Claims (2)
1. a kind of up-conversion fluorescence system for detecting temperature of laser pump (ing), it is characterised in that: by the first semiconductor laser, second
Semiconductor laser, sample stage, loop temperature-control device, body phase thermo detector, optical filter, CCD detector and computer composition；Wherein,
Sample stage is for placing fluorescence temperature probe material, Tissue Culture Dish, living animal and tissue；First semiconductor laser is made
For the fluorescence excitaton source of fluorescence temperature probe, it is emitted optical registration sample stage, the second semiconductor laser is visited as fluorescence temperature
The photic heating source of needle is directed at sample stage；Loop temperature-control device surrounds sample stage, and body phase thermo detector passes through temperature probe and sample stage
Connection, loop temperature-control device and body phase thermo detector are for being measured the light intensity of fluorescence temperature probe with temperature dependency curve；Sample
Optical filter is placed in the front of sample platform, and the light-emitting window of optical filter is directed at CCD detector, and CCD detector passes through signal receiving circuit
Connect computer；First semiconductor laser uses central wavelength for the semiconductor laser of 980nm, and described the second half lead
Body laser uses central wavelength for the semiconductor laser of 730nm.
2. the up-conversion fluorescence system for detecting temperature of laser pump (ing) according to claim 1, it is characterised in that the optical filtering
Piece uses the short pass filter of 720nm or 850nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610005174.6A CN105547516B (en) | 2016-01-07 | 2016-01-07 | Laser pump (ing) up-conversion fluorescence temp measuring system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610005174.6A CN105547516B (en) | 2016-01-07 | 2016-01-07 | Laser pump (ing) up-conversion fluorescence temp measuring system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105547516A CN105547516A (en) | 2016-05-04 |
| CN105547516B true CN105547516B (en) | 2019-11-12 |
Family
ID=55826866
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610005174.6A Active CN105547516B (en) | 2016-01-07 | 2016-01-07 | Laser pump (ing) up-conversion fluorescence temp measuring system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105547516B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106017722B (en) * | 2016-08-10 | 2018-10-09 | 中国工程物理研究院流体物理研究所 | A kind of single-point fluorescence temperature measuring equipment and temp measuring method |
| CN106768406B (en) * | 2016-11-24 | 2019-03-26 | 哈尔滨工业大学 | A kind of cell temperature measuring system |
| CN108489617B (en) * | 2018-03-22 | 2019-08-02 | 哈尔滨工业大学 | A method of improving thulium ion near-infrared up-conversion fluorescence sensitivity of thermometry |
| CN111879434B (en) * | 2020-07-24 | 2022-04-08 | 之江实验室 | Method and device for measuring temperature of biological tissue or cell |
| CN113483911A (en) * | 2021-04-14 | 2021-10-08 | 天津大学 | Optical fiber temperature sensor based on nano fluorescent material and measuring system |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000283859A (en) * | 1999-03-30 | 2000-10-13 | Osaka Gas Co Ltd | Method and apparatus for measuring surface temperature of object |
| JP3379352B2 (en) * | 1996-09-09 | 2003-02-24 | 株式会社豊田中央研究所 | Method and apparatus for measuring two-dimensional temperature distribution of fluid |
| CN1584553A (en) * | 2004-06-04 | 2005-02-23 | 湖南大学 | Measuring apparatus and method for fluorescent material temperature-changing characteristic |
| CN101611298A (en) * | 2006-12-22 | 2009-12-23 | 索尼德国有限责任公司 | With temperature in high-resolution sensing microscopic electronic devices and the biosome and Temperature Distribution |
| CN101718695A (en) * | 2009-12-07 | 2010-06-02 | 浙江大学 | Device for measuring temperature characteristics of fluorescent powder |
| CN202184721U (en) * | 2011-05-27 | 2012-04-11 | 浙江大学 | Lymph gland and blood vessel upper conversion fluorescent imaging system |
| JP2012177549A (en) * | 2009-06-24 | 2012-09-13 | Hokkaido Univ | Fluorescent temperature probe and temperature measuring instrument using the same |
| CN102798485A (en) * | 2012-08-14 | 2012-11-28 | 厦门大学 | Temperature sensing method based on upconversion fluorescent powder |
| CN104316214A (en) * | 2014-09-22 | 2015-01-28 | 西北大学 | Application of ALn(MoO4)2 up-conversion luminescent material doped with Er3+ and Yb3+ in optical temperature sensor |
| CN205449294U (en) * | 2016-01-07 | 2016-08-10 | 复旦大学 | Last fluorescence temperature measurement system that changes of laser pumping |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948694B (en) * | 2010-09-08 | 2013-04-17 | 吉林大学 | Rare earth upconversion nano crystal/silver difunctional composite nano material, preparation method thereof and application thereof in cancer detection and treatment |
| CN102952524B (en) * | 2012-03-01 | 2015-04-22 | 纳米籽有限公司 | Micro-domain photothermal composite material and preparation method thereof |
| CN104019922B (en) * | 2014-04-29 | 2016-11-23 | 北京理工大学 | Hybrid inorganic-organic perovskite material is used for temperature sensing by one |
| CN104017581B (en) * | 2014-06-18 | 2015-09-09 | 东南大学 | Photothermal deformation nano material and preparations and applicatio method thereof |
| CN104371726B (en) * | 2014-11-26 | 2016-08-17 | 中国计量学院 | It is nanocrystalline and its preparation method and application that a kind of high temperature that can be used for temperature sensing strengthens up-conversion fluorescence type |
-
2016
- 2016-01-07 CN CN201610005174.6A patent/CN105547516B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3379352B2 (en) * | 1996-09-09 | 2003-02-24 | 株式会社豊田中央研究所 | Method and apparatus for measuring two-dimensional temperature distribution of fluid |
| JP2000283859A (en) * | 1999-03-30 | 2000-10-13 | Osaka Gas Co Ltd | Method and apparatus for measuring surface temperature of object |
| CN1584553A (en) * | 2004-06-04 | 2005-02-23 | 湖南大学 | Measuring apparatus and method for fluorescent material temperature-changing characteristic |
| CN101611298A (en) * | 2006-12-22 | 2009-12-23 | 索尼德国有限责任公司 | With temperature in high-resolution sensing microscopic electronic devices and the biosome and Temperature Distribution |
| JP2012177549A (en) * | 2009-06-24 | 2012-09-13 | Hokkaido Univ | Fluorescent temperature probe and temperature measuring instrument using the same |
| CN101718695A (en) * | 2009-12-07 | 2010-06-02 | 浙江大学 | Device for measuring temperature characteristics of fluorescent powder |
| CN202184721U (en) * | 2011-05-27 | 2012-04-11 | 浙江大学 | Lymph gland and blood vessel upper conversion fluorescent imaging system |
| CN102798485A (en) * | 2012-08-14 | 2012-11-28 | 厦门大学 | Temperature sensing method based on upconversion fluorescent powder |
| CN104316214A (en) * | 2014-09-22 | 2015-01-28 | 西北大学 | Application of ALn(MoO4)2 up-conversion luminescent material doped with Er3+ and Yb3+ in optical temperature sensor |
| CN205449294U (en) * | 2016-01-07 | 2016-08-10 | 复旦大学 | Last fluorescence temperature measurement system that changes of laser pumping |
Non-Patent Citations (2)
| Title |
|---|
| Er3+/Yb3+共掺Y2O3材料的上转换荧光温度特性研究;尹爽;《中国优秀硕士学位论文全文数据库基础科学辑》;20150215;第18-25页 * |
| Tm3+，Yb3+共掺钨酸钙多晶材料的上转换发光及荧光温度特性;郑龙江等;《物理学报》;20131231;第62卷(第24期);第1-6页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105547516A (en) | 2016-05-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105547516B (en) | Laser pump (ing) up-conversion fluorescence temp measuring system | |
| Bednarkiewicz et al. | Standardizing luminescence nanothermometry for biomedical applications | |
| Yu et al. | One ion to catch them all: Targeted high-precision Boltzmann thermometry over a wide temperature range with Gd3+ | |
| Suo et al. | All-in-one thermometer-heater up-converting platform YF 3: Yb 3+, Tm 3+ operating in the first biological window | |
| Rurack et al. | Fluorescence quantum yields of a series of red and near-infrared dyes emitting at 600− 1000 nm | |
| Chihara et al. | Biological deep temperature imaging with fluorescence lifetime of rare-earth-doped ceramics particles in the second NIR biological window | |
| Maturi et al. | Going above and beyond: a tenfold gain in the performance of luminescence thermometers joining multiparametric sensing and multiple regression | |
| Würth et al. | Evaluation of a commercial integrating sphere setup for the determination of absolute photoluminescence quantum yields of dilute dye solutions | |
| Hyppänen et al. | Environmental and excitation power effects on the ratiometric upconversion luminescence based temperature sensing using nanocrystalline NaYF4: Yb3+, Er3+ | |
| Shahi et al. | Lanthanide complexes for temperature sensing, UV light detection, and laser applications | |
| Sordillo et al. | Optical spectral fingerprints of tissues from patients with different breast cancer histologies using a novel fluorescence spectroscopic device | |
| CN102798485A (en) | Temperature sensing method based on upconversion fluorescent powder | |
| CN108896201A (en) | A kind of high-resolution organism thermometry of high sensitivity | |
| Nawara et al. | Goodbye to quinine in sulfuric acid solutions as a fluorescence quantum yield standard | |
| Liu et al. | Ultrasensitive thermochromic upconversion in core–shell–shell nanoparticles for nanothermometry and anticounterfeiting | |
| Chu et al. | Fluorescence ratiometric optical broad range pH sensor based on CdSe/ZnS quantum dots and O170 embedded in ethyl cellulose matrix | |
| US20160313249A1 (en) | Calibration standard for a device for image-based representation of biological material | |
| CN205449294U (en) | Last fluorescence temperature measurement system that changes of laser pumping | |
| Würth et al. | Polymer-and glass-based fluorescence standards for the near infrared (NIR) spectral region | |
| US20130197327A1 (en) | Analyte sensor | |
| JP2021514051A (en) | Analysis equipment | |
| US20090326358A1 (en) | Non-invasive fast-response biodosimeter | |
| Farago et al. | A side-polished fluorescent fiber sensor for the detection of blood in the saliva | |
| Pauli et al. | Fluorescence Quantum Yield Standards for the UV/Visible/NIR: Development, Traceable Characterization, and Certification | |
| Zhang et al. | Use of the fluorescence of rhodamine B for the pH sensing of a glycine solution |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |