CN105541831A - Medicine root berberrubine derived from amur cork-tree processed product and separation preparation method and application thereof - Google Patents

Medicine root berberrubine derived from amur cork-tree processed product and separation preparation method and application thereof Download PDF

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CN105541831A
CN105541831A CN201610026002.7A CN201610026002A CN105541831A CN 105541831 A CN105541831 A CN 105541831A CN 201610026002 A CN201610026002 A CN 201610026002A CN 105541831 A CN105541831 A CN 105541831A
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medicine root
red alkali
medicine
golden cypress
processed product
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CN105541831B (en
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张凡
刘蓬蓬
贾天柱
徐珊
高慧
史辑
鞠成国
林桂梅
单国顺
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Liaoning University of Traditional Chinese Medicine
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D455/00—Heterocyclic compounds containing quinolizine ring systems, e.g. emetine alkaloids, protoberberine; Alkylenedioxy derivatives of dibenzo [a, g] quinolizines, e.g. berberine
    • C07D455/03—Heterocyclic compounds containing quinolizine ring systems, e.g. emetine alkaloids, protoberberine; Alkylenedioxy derivatives of dibenzo [a, g] quinolizines, e.g. berberine containing quinolizine ring systems directly condensed with at least one six-membered carbocyclic ring, e.g. protoberberine; Alkylenedioxy derivatives of dibenzo [a, g] quinolizines, e.g. berberine

Abstract

The invention provides medicine root berberrubine derived from an amur cork-tree processed product and a separation preparation method and application thereof. The method includes the steps that 1, jatrorrhizine hydrochloride reference substances are precisely weighed, placed in an evaporation dish to be baked, taken out and cooled, chromatographic grade methyl alcohol is added, millipore filter filtering is conducted, and a baked jatrorrhizine reference substance solution is obtained; 2, the baked jatrorrhizine reference substance solution is separated through a preparative liquid phase, eluant of a target chromatographic peak is collected and purified through a sephadex column, the purified eluant is subjected to nitrogen blowing and freeze drying, and pink powder, namely, medicine root berberrubine crystals prepared through separation are obtained. The prepared medicine root berberrubine is a new compound found in amur cork-tree processed decoction pieces, the separation preparation method has the advantages of being high in efficiency, stable in process, easy and convenient to operate and low in cost, high-purity separation preparation of a large number of medicine root berberrubine monomers can be achieved, and the medicine root berberrubine compound has good macrophage phagocytosis and high immunization.

Description

A kind of come from golden cypress processed product the red alkali of medicine root and method for separating and preparing and purposes
Technical field
The present invention relates to technical field of Chinese medicine, particularly relate to a kind of come from golden cypress processed product the red alkali of medicine root and method for separating and preparing and purposes.
Background technology
Golden cypress begins to be loaded in Shennong's Herbal, is rutaceae wampee phellodendronor cork tree chinenseSchneid. phellodendronamurenseRupr. dry bark, the medicinal history of existing more than 2,000 year is traditional Chinese medical science tradition heat-clearing and fire-purging drug.Ancient Chinese medicine doctor attaches great importance to the process of preparing Chinese medicine to golden cypress in clinical application, and its processed product sees Shanxi generation the earliest, the handbook of Prescription for Emergency of Ge Hong.Traditional concocting method of golden cypress has 16 kinds of concocting methods such as clean system, cutting, honey system, wine system, salt system, charcoal system, milk system, boys' urine, is main now mainly with wine system, salt system and charcoal processing.
Main containing alkaloids compositions such as Berberine, palmatine, jateorhizine, Phellodendrines in golden cypress.Modern pharmacology Effect study shows that golden cypress has hypoglycemic, step-down, the effect such as anticancer, anti-oxidant.Golden cypress affecting its chemical composition certain change can occur due to temperature in concocting process, and because of after adding different auxiliary materials, its property of medicine also can change to some extent, finally causes the difference of pharmacological action.By comparing the raw product of golden cypress, the high-efficient liquid phase chromatogram that product processed by wine, product processed by salt finds, golden cypress has new chromatographic peak to generate after concocting.This laboratory early-stage Study result shows, golden cypress has two chemical compositions to there occurs change in concocting process, one of them is the berberrubine be transformed by Berberine, another is the bar Ma Hongting be transformed by palmatine, the content simultaneously observing jateorhizine decreases after the process of preparing Chinese medicine, therefore heat treated has been carried out to jateorhizine monomer, find that there is a newly-generated chromatographic peak and this peak concoct with golden cypress after high-efficient liquid phase chromatogram on a newly-generated chromatographic peak there is identical retention time.Thus, infer that in golden cypress processed product, this newly-generated chemical composition is that in golden cypress, jateorhizine is transformed in concocting process.Binding experiment room early-stage Study result, for whole observation golden cypress concocts alkaloid change and the Changing Pattern of Protoberberine Alkoloids in concocting process of front and back, therefore to jateorhizine (C 20h 20nO 4) the process of preparing Chinese medicine before and after the new constituent of chemical conversion be prepared separation.
Summary of the invention
For the problems referred to above, the invention provides a kind of come from golden cypress processed product the red alkali of medicine root and method for separating and preparing and purposes, the method is separated from golden cypress processed product prepares the red alkali of medicine root, have that separation efficiency is high, process stabilizing, easy and simple to handle, with low cost, the high-content high purity (more than 98%) that can realize the red alkali monomer of a large amount of medicine root is separated preparation.
For realizing above-mentioned purpose of the present invention, the invention provides a kind of red alkali of medicine root coming from golden cypress processed product, molecular formula is C 19h 17nO 4, the red alkali of called after medicine root, chemical structural formula is as follows.
The present invention also provides a kind of method for separating and preparing coming from the red alkali of medicine root of golden cypress processed product, comprises the following steps.
The simulation of step 1, jateorhizine is concocted: precision takes Jatrorrhizine chloride reference substance, puts in furnace pot, at 160 ~ 216 DEG C, smoke 15 ~ 25min, taking-up cools, add hplc grade methanol, then millipore filtration (0.45 μm) filters, the jateorhizine reference substance solution after must smoking.
Step 2, jateorhizine monomer smoke separation and the preparation of product new constituent.
Preparative liquid chromatography condition: mobile phase A is water (wherein 100ml water is containing 0.4ml diethylamine), and B is methyl alcohol; The mobile phase ratio of flow rate of mobile phase: A and B is (70 ~ 90:30 ~ 10, volume ratio); With anti-phase C 18bonded silica stationary phase is chromatographic column filler; Column temperature 25 DEG C; Ultraviolet detection wavelength is 220 ~ 345nm; Sample size is 100 μ L.
Jateorhizine reference substance solution preparation liquid phase after smoking is separated, collect target chromatographic peak elutriant and through dextrane gel column purification; Purified elutriant is carried out nitrogen and blows 15min, lyophilize at putting-80 DEG C, pink powder to be obtained, be be separated preparation the red alkali crystallization of medicine root.
The red alkali of described medicine root is for the preparation of immunoregulatory medicine.
Compared with prior art beneficial effect of the present invention.
Provided by the invention come from golden cypress processed product the red alkali of medicine root and method for separating and preparing and purposes, the prepared red alkali of medicine root is concoct golden cypress the new compound found in medicine materical crude slice, this preparative separation method has that efficiency is high, process stabilizing, feature easy and simple to handle, with low cost, the high purity (more than 98%) that can realize the red alkali monomer of a large amount of medicine root is separated preparation, and the red alkali cpd of this medicine root has the phagolysis of good scavenger cell, there is stronger immunization, relative to alkaloid original in golden cypress, there is stronger immune effect.
Accompanying drawing explanation
Fig. 1 is the preparative liquid chromatography figure of the red alkali of medicine root, and wherein A is the red alkali of medicine root.
Fig. 2 is the red alkali of medicine root 1h-NMR composes.
Fig. 3 is the red alkali of medicine root 13c-NMR composes.
Fig. 4 is different concns jateorhizine and the relative phagocytic rate of the red alkali of medicine root to cell.
Embodiment
Further describe the present invention below in conjunction with specific embodiment, but this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following embodiment, all technology realized based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1.
1, instrument and material.
1.1 instruments: semi-preparative liquid chromatography instrument (Shimadzu LC-10AT); METTLERAE240 type 100,000/analytical balance (Switzerland METTLER); FA1004B type electronic balance (Shanghai Precision Scientific Apparatus Co., Ltd); YP5102 type electronic balance (the upper positive Medical Instruments company limited of sea light); KQ-250DB type numerical control ultrasonic cleaner (Kunshan Ultrasonic Instruments Co., Ltd.); Nitrogen evaporator (Tianjin Hengao Technology Development Co., Ltd.); 101 type electric drying oven with forced convections (Beijing is bright Medical Instruments factory forever); CHRIST vacuum freeze drier (Germany); The semi-automatic point sample instrument of CAMAGLinomat-5 type (Switzerland).
1.2 reagents: golden cypress medicinal material is purchased from medicinal material company of Sichuan Province; Jatrorrhizine chloride reference substance is purchased from Wei Keqi bio tech ltd of Sichuan Province (lot number 130218); The red alkali of medicine root is laboratory self-control, through high effective liquid chromatography for measuring, and purity > 98%; Thin-layer chromatography silica-gel plate (Haiyang Chemical Plant, Qingdao); SephadexLH-20 (Pharmacia); Methyl alcohol analytical pure (Tianjin great Mao chemical reagent factory); Acetonitrile, methyl alcohol chromatographically pure; Diethylamine analytical pure (Tianjin great Mao chemical reagent factory); Water is pure water.
2, the present embodiment provides a kind of method for separating and preparing coming from the red alkali of medicine root of golden cypress processed product, comprises the following steps.
Step 1, the index composition jateorhizine reference substance in golden cypress carried out simulation and concoct: precision takes Jatrorrhizine chloride reference substance 5mg, put in furnace pot, 20min is smoked at 216 DEG C, taking-up cools, add hplc grade methanol 5mL, then millipore filtration (0.45 μm) filters, and the jateorhizine reference substance solution after must smoking, carries out the preparation of new constituent.
Step 2, jateorhizine monomer smoke separation and the preparation of product new constituent, refer to Fig. 1.
Preparative liquid chromatography condition: mobile phase A is water, wherein 100ml water is containing 0.4ml diethylamine, and B is methyl alcohol; The mobile phase ratio of flow rate of mobile phase: A and B is (80:20); With anti-phase C 18bonded silica stationary phase is chromatographic column filler; Column temperature 25 DEG C; Ultraviolet detection wavelength is 345nm; Sample size is 100 μ L.
Jateorhizine reference substance solution preparation liquid phase after smoking is separated, collect target chromatographic peak elutriant and through dextrane gel column purification; Purified elutriant is carried out nitrogen and blows 15min, lyophilize at putting-80 DEG C, pink powder to be obtained, be be separated preparation the red alkali crystallization of medicine root.
3, the described qualification being separated the red alkali cpd of medicine root of preparation, refers to Fig. 2 and Fig. 3.
Be separated the composition obtained, warp 1h-NMR, 13c-NMR Analysis and Identification. 1h-NMR (MeOD, 300MHz) δ: δ H:7.53 (1H, s, H-1), 6.80 (1H, s, H-4), 3.12 (2H, t, H-5), 4.65 (2H, t, H-6), 9.34 (1H, s, H-8), 7.62 (1H, d, H-11), 7.01 (1H, d, H-12), 8.22 (1H, s, H-13), 3.93 (3H, s, 2-OCH3), 3.99 (3H, s, 2-OCH3), 13C-NMR (MeOD, 75MHz) δ: δ C:109.5 (C-1), 149.4 (C-2), 150.6 (C-3), 115.9 (C-4), 28.4 (C-5), 56.0 (C-6), 147.3 (C-8), 124.9 (C-8a), 169.3 (C-9), 57.0 (10-OCH3), 129.9 (C-11), 129.5 (C-12), 134.3 (C-12a), 120.6 (C-13), 136.6 (C-14), 119.3 (C-14a), gained is transformed and outward appearance pinkiness according to the structure of above data and document determination new constituent by jateorhizine, therefore by the red alkali of this new constituent called after medicine root, molecular formula is C 19h 17nO 4.Described jateorhizine path for transformation is as follows.
4, be verify beneficial effect of the present invention further, the test case of following " Activity determination of the red alkali of medicine root is to the immunoregulation effect of the scavenger cell stimulated through LPS " is provided.
4.1 measuring method.
The RAW264.7 cell of logarithmic phase, after digestion, scraping, piping and druming, adjusts cell density to 1 × 10 7individual/mL, and be inoculated on 96 well culture plates, every pore volume 100 μ L.Put 37 DEG C, 5%CO 2after incubator hatches 24h, abandon supernatant liquor.Be divided into 3 groups, i.e. blank group: add blank nutrient solution 200 μ L; Model control group: the LPS100 μ L adding 2 μ g/mL, and to be diluted to final volume with blank nutrient solution be 200 μ L; Administration group: adding 7 different concns containing jateorhizine and the dosing nutrient solution 100 μ L of the red alkali of medicine root and the LPS100 μ L(LPS final concentration of 2 μ g/mL is 1 μ g/mL) final volume is 200 μ L, each concentration establishes 3 multiple holes.37 DEG C, 5%CO 2after cultivating 24h in incubator, after abandoning culture supernatant, add 0.1% neutral red solution 200 μ L, 37 DEG C, 5%CO 2hatch 60min, abandon toluylene red, wash 3 times, each 200 μ L with the PBS of pre-temperature, and dry.Add acetic acid-ethanol (1:1, volume ratio) cytolysate 200 μ L/ hole, 4 DEG C of hold over night, microplate reader 570nm place surveys OD value.And calculate the relative phagocytic rate of cell (RSR).
The relative phagocytic rate of cell (%)=(the blank group of OD experimental group-OD)/(the blank group of OD control group-OD) × 100%.
Adopt SPSS19.0 software to carry out statistical procedures, measurement data represents with " means standard deviation ", and employing t inspection is compared between organizing, and P < 0.05 is variant, has statistical significance.
4.2 measurement results, refer to table 1 and Fig. 4.
Table 1: jateorhizine and the red alkali of medicine root are on the impact of RAW264.7 macrophage phagocytic.
Group OD 570 Relative phagocytic rate (%)
Blank group 0.192±0.004 —
Control group 0.324±0.004 —
100 μ g/mL jateorhizines 0.316±0.008 93.94
The red alkali of 100 μ g/mL medicine root 0.506±0.048** 237.88
50 μ g/mL jateorhizines 0.368±0.015 133.33
The red alkali of 50 μ g/mL medicine root 0.596±0.059** 306.06
25 μ g/mL jateorhizines 0.474±0.064 213.64
The red alkali of 25 μ g/mL medicine root 0.628±0.045** 330.30
10 μ g/mL jateorhizines 0.566±0.013 283.33
The red alkali of 10 μ g/mL medicine root 0.709±0.075* 391.67
5 μ g/mL jateorhizines 0.590±0.022 301.52
The red alkali of 5 μ g/mL medicine root 0.736±0.088** 412.12
2.5 μ g/mL jateorhizines 0.473±0.015 212.88
The red alkali of 2.5 μ g/mL medicine root 0.635±0.062* 335.61
1 μ g/mL jateorhizine 0.293±0.096 76.52
The red alkali of 1 μ g/mL medicine root 0.420±0.007** 172.73
* is compared with jateorhizine p< 0.05, * * p< 0.01.
Result shows, the red alkali of medicine root has stronger promoter action compared with jateorhizine to macrophage phagocytic effect, illustrates that the red alkali of medicine root is more of value to compared with jateorhizine phagocytic cell to affect wound healing process at inflammation phase scavenging machine bulk damage place tissue and the Necrotic Debris of cell and pathogenic agent.
5, jateorhizine, the red alkali of medicine root are frying the assay in golden cypress, phellodendron bark, stir-baking CORTEX PHYLLODENDRI with yellow rice wine, stir-baked CORTEX PHELLODENDRI with salt solution.
The preparation of the red alkali reference substance solution of 5.1 jateorhizines, medicine root.
Precision takes jateorhizine 2.06mg, puts in 50mL volumetric flask, dissolves and is diluted to scale, makes the reference substance solution that concentration is 0.0412mg/mL.
Precision takes isolated new constituent, and the red alkali 0.72mg of medicine root, puts in 10mL volumetric flask, dissolve and be diluted to scale, makes the reference substance solution that concentration is 0.0720mg/mL.
The preparation of 5.2 trial-products.
Fry golden cypress and get clean golden cypress medicine materical crude slice 50g, in pot, fry 6min, take out, pulverize, cross 60 mesh sieves.
Phellodendron bark gets clean golden cypress medicine materical crude slice 50g, pulverizes, and crosses 60 mesh sieves.
Stir-baking CORTEX PHYLLODENDRI with yellow rice wine gets clean golden cypress medicine materical crude slice 50g, and add yellow rice wine (wine of 20% medicine materical crude slice amount, the water of 15% medicine materical crude slice amount) and mix thoroughly, vexed profit 12h, puts frying 6min in 150 ~ 160 DEG C of iron pans, takes out, cools, and pulverizes, and crosses 60 mesh sieves.
Stir-baked CORTEX PHELLODENDRI with salt solution gets clean golden cypress medicine materical crude slice 50g, adds salt solution (salt of 2% medicine materical crude slice amount, the water of 25% medicine materical crude slice amount, cool), mixes thoroughly, vexed profit, put frying 6min in 150 ~ 160 DEG C of iron pans, takes out, cools, and pulverizes, and crosses 60 mesh sieves.
Get and fry golden cypress, phellodendron bark, stir-baking CORTEX PHYLLODENDRI with yellow rice wine, stir-baked CORTEX PHELLODENDRI with salt solution powder be about 0.5g, accurately weighed, put in tool plug Erlenmeyer flask, weighed weight, supersound process 60min, take out, let cool, supply the weight of minimizing with extraction solution, shake up, hold over night, filters, gets subsequent filtrate, to obtain final product.
5.3 experimental result.
Get each need testing solution respectively appropriate, measure, the results are shown in Table 2 according to following chromatographic condition, wherein ,-expression does not detect this composition.
Chromatographic condition: Waterse2695 type high performance liquid chromatograph; Chromatographic column: ECOSILC 18(5 μm, 4.6 × 250mm); Flow velocity: 1mL/min; Sample size: 30 μ L.
Table 2: fry jateorhizine and the red alkali content of medicine root in golden cypress, phellodendron bark, stir-baking CORTEX PHYLLODENDRI with yellow rice wine, stir-baked CORTEX PHELLODENDRI with salt solution.
Sample Simultaneous Determinat ion (%) The red alkali content of medicine root (%) Transformation efficiency (%)
Fry golden cypress 0.023 0.039 26.0
Phellodendron bark 0.150 —
Stir-baking CORTEX PHYLLODENDRI with yellow rice wine 0.390 0.012 8.0
Stir-baked CORTEX PHELLODENDRI with salt solution 0.466 0.023 15.3
Result shows, in stir-baking CORTEX PHYLLODENDRI with yellow rice wine, stir-baked CORTEX PHELLODENDRI with salt solution, Simultaneous Determinat ion is obviously more than the content of jateorhizine in phellodendron bark, fries Simultaneous Determinat ion in golden cypress and is less than Simultaneous Determinat ion in phellodendron bark.The red alkali of medicine root do not detected in phellodendron bark, and fry in golden cypress, stir-baking CORTEX PHYLLODENDRI with yellow rice wine, stir-baked CORTEX PHELLODENDRI with salt solution and the red alkali of medicine root all detected and to fry the red alkali content of medicine root generated in product more, stir-baked CORTEX PHELLODENDRI with salt solution takes second place, and stir-baking CORTEX PHYLLODENDRI with yellow rice wine is minimum.Phellodendron bark is after frying, and it is the highest that jateorhizine transforms the transformation efficiency generating the red alkali of medicine root.
Embodiment 2.
The present embodiment provides a kind of method for separating and preparing coming from the red alkali of medicine root of golden cypress processed product, comprises the following steps.
Step 1, the index composition jateorhizine reference substance in golden cypress carried out simulation and concoct: precision takes Jatrorrhizine chloride reference substance 5mg, put in furnace pot, 15min is smoked at 160 DEG C, taking-up cools, add hplc grade methanol 5mL, millipore filtration (0.45 μm) filters, and the jateorhizine reference substance solution after must smoking, carries out the preparation of new constituent.
Step 2, jateorhizine monomer smoke separation and the preparation of product new constituent.
Preparative liquid chromatography condition: mobile phase A is water, wherein 100ml water is containing 0.4ml diethylamine, and B is methyl alcohol; The mobile phase ratio of flow rate of mobile phase: A and B is (70:30); With anti-phase C 18bonded silica stationary phase is chromatographic column filler; Column temperature 25 DEG C; Ultraviolet detection wavelength is 220nm; Sample size is 100 μ L.
Jateorhizine reference substance solution preparation liquid phase after smoking is separated, collect target chromatographic peak elutriant and through dextrane gel column purification; Purified elutriant is carried out nitrogen and blows 15min, lyophilize at putting-80 DEG C, pink powder to be obtained, be be separated preparation the red alkali crystallization of medicine root.
Embodiment 3.
The present embodiment provides a kind of method for separating and preparing coming from the red alkali of medicine root of golden cypress processed product, comprises the following steps.
Step 1, the index composition jateorhizine reference substance in golden cypress carried out simulation and concoct: precision takes Jatrorrhizine chloride reference substance 5mg, put in furnace pot, 25min is smoked at 180 DEG C, taking-up cools, add hplc grade methanol 5mL, millipore filtration (0.45 μm) filters, and the jateorhizine reference substance solution after must smoking, carries out the preparation of new constituent.
Step 2, jateorhizine monomer smoke separation and the preparation of product new constituent.
Preparative liquid chromatography condition: mobile phase A is water, wherein 100ml water is containing 0.4ml diethylamine, and B is methyl alcohol; The mobile phase ratio of flow rate of mobile phase: A and B is (90:10); With anti-phase C 18bonded silica stationary phase is chromatographic column filler; Column temperature 25 DEG C; Ultraviolet detection wavelength is 265nm; Sample size is 100 μ L.
Jateorhizine reference substance solution preparation liquid phase after smoking is separated, collect target chromatographic peak elutriant and through dextrane gel column purification; Purified elutriant is carried out nitrogen and blows 15min, lyophilize at putting-80 DEG C, pink powder to be obtained, be be separated preparation the red alkali crystallization of medicine root.

Claims (6)

1. come from the red alkali of medicine root of golden cypress processed product, it is characterized in that, molecular formula is C 19h 17nO 4, the red alkali of called after medicine root, chemical structural formula is as follows.
2. come from the red alkali of medicine root of golden cypress processed product as claimed in claim 1, it is characterized in that, comprise the following steps:
The simulation of step 1, jateorhizine is concocted: precision takes Jatrorrhizine chloride reference substance, puts in furnace pot and smokes, and taking-up cools, and adds hplc grade methanol, and millipore filtration (0.45 μm) filters, the jateorhizine reference substance solution after must smoking;
Step 2, jateorhizine monomer smoke separation and the preparation of product new constituent:
Preparative liquid chromatography condition: mobile phase A is water, B is methyl alcohol; The mobile phase ratio of flow rate of mobile phase: A and B is (70 ~ 90:30 ~ 10); With anti-phase C 18bonded silica stationary phase is chromatographic column filler; Column temperature 25 DEG C; Ultraviolet detection wavelength is 220 ~ 345nm; Sample size is 100 μ L;
Jateorhizine reference substance solution preparation liquid phase after smoking is separated, collect target chromatographic peak elutriant and through dextrane gel column purification; Purified elutriant is carried out nitrogen blow, lyophilize, pink powder to be obtained, be be separated preparation the red alkali crystallization of medicine root.
3. come from the red alkali of medicine root of golden cypress processed product as claimed in claim 2, it is characterized in that, in described step 1, baked temperature is 160 ~ 216 degree, and baking time is 15 ~ 25min.
4. come from the red alkali of medicine root of golden cypress processed product as claimed in claim 4, it is characterized in that, described mobile phase A is water, and wherein 100ml water is containing 0.4ml diethylamine.
5. come from the method for separating and preparing of the red alkali of medicine root of golden cypress processed product as claimed in claim 1, it is characterized in that, the described purity being separated the red alkali of medicine root of preparation is more than 98%.
6. come from the purposes of the red alkali of medicine root of golden cypress processed product as claimed in claim 1, it is characterized in that, the red alkali of described medicine root is used for immunomodulatory.
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CN108014517A (en) * 2017-12-20 2018-05-11 泰州医药城国科化物生物医药科技有限公司 A kind of method using polysaccharide-based medium to Protoberberine Alkoloids enriching and purifying
CN107951946A (en) * 2018-01-10 2018-04-24 辽宁中医药大学 A kind of preparation method of the Cortex Phellodendri processed product extract of anti-oral ulcer
CN107951946B (en) * 2018-01-10 2021-03-16 辽宁中医药大学 Preparation method of cortex phellodendri processed product extract for resisting oral ulcer

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