CN105536699A - Method for preparing printing and dyeing wastewater decolorization agent from modified reed stalks - Google Patents

Abstract

The invention discloses a method for preparing a printing and dyeing wastewater decolorization agent from modified reed stalks, and belongs to the technical field of wastewater treatment. Aiming at the problems that in a conventional traditional treatment of printing and dyeing wastewater, the treatment cost is relatively high, regeneration is difficult and impurities are easy to introduce to cause secondary pollution, reed stalks are used as a main body, then are subjected to modification carbonization, next are subjected to biological modification, and are supplemented with an additive and mixed, and thus the printing and dyeing wastewater decolorization agent which can be renewable for use and low in preparation cost is prepared. The method is low in preparation cost and easy to operate, the decolorization effect reaches more than or equal to 80%, and the decolorization agent can be repeatedly used and is easy to activate.

Description

A kind of modification reed rod prepares the method for decolorizing printing and dyeing waste water agent

Technical field

The invention discloses a kind of method that modification reed rod prepares decolorizing printing and dyeing waste water agent, belong to technical field of waste water processing.

Background technology

Weaving, dyeing are one of China's leading industrys, although a large amount of labours has been received in thousands of weavings, printing and dyeing, but the waste water from dyestuff of its discharge simultaneously causes serious threat to environment and water safety, particularly in enterprise's sewage drainage standard that all demand fulfillment is strict of south water to north weaving along the line, printing and dyeing, otherwise the destiny of stopping production will be faced.In addition dye component more and more trends towards anti-oxidant, anti-light solution, antibiont degraded, and its COD concentration and colourity all more and more higher, so it is progressive just to require that dye wastewater treatment technology needs constantly.In addition, containing groups such as a large amount of phenyl ring, naphthalene nucleus, amino and azos in dye molecule, its factory effluent complicated component, colourity is dark, has peculiar smell, organic matter and inorganic salt content high, great threat can be produced, so waste water from dyestuff Environmental capacity is extremely urgent to health.

Along with the development of chemical engineering industry, dye species increases, and structurally number of rings increases, and carbochain increases, and dye radical is a lot, and the BOD/COD ratio of waste water declines, and thus waste water becomes and is difficult to biochemical treatment.But also have the method for a lot of dye wastewater treatment at present, absorption method, Coagulation Method, magnetic separation method, chemical oxidization method, electrochemical process and biochemical process etc., these method pluses and minuses all clearly.

For absorption method, after its process, water quality is good and stable, there is no secondary pollution, in dye wastewater treatment, there is consequence, conventional adsorbent comprises active carbon and resin etc., this method is very effective to dissolved organic matter in removal water, but reproduction ratio is more difficult, processing cost is higher, application surface is narrow.Coagulation Method is most economical, the most effective method of current dye wastewater treatment, and its major advantage is that equipment investment is few, floor space is few, technological process is simple, convenient operation and management, very high to hydrophobic dye decolorizing efficiency; Shortcoming is that operating cost is higher, body refuse amount is large and dehydration because pH value range that is difficult, that be suitable for is narrow, to hydrophilic dye poor processing effect.Magnetic separation method is that the particulate in water body is magnetized the method be separated again in advance.As a kind of water treatment new technology, magnetic agglomeration process, molysite coprecipitation, Powder by Iron Powder, ferrite process etc. can be mainly contained for the magnetization technology of industrial application now.The major way that membrane separation process is applied to azo dye wastewater process is ultrafiltration and counter-infiltration.In ultra-filtration process, film surface pore size is major control factors; Counter-infiltration is that to apply certain pressure be that motive force realizes being separated of water and dyestuff on pellicle, and carries out dyestuff recovery.Chemical oxidization method degrading azoic dye waste water utilizes strong oxidizer oxidation dye molecule, destroys its chromophore, reduces colourity and the COD value of waste water, improve the biodegradability of waste water, reach a kind of method of purifying liquid waste.The subject matter that chemical oxidization method exists is that processing cost is high; Catalyst cannot reclaim; It is not strong that conventional oxidant shows oxidability, there is the shortcomings such as selective oxidation; And easy impurity of introducing causes secondary pollution in processing procedure.Electrochemical process for treating is the advanced oxidation technology that latest developments are got up, utilize DC Electric Field in specific electrochemical reactor, through the chemical reaction of a series of design, electrochemical process or physical process, reach the removal Pollutants in Wastewater of expection or reclaim the object of utility.Adopt electrochemical method process printing/dyeing waste water, have equipment little, take up an area less, operational management is simple, COD clearance is high and the advantage such as good decolorizing effect, is a kind of " environmental friendliness " technology.Biological treatment has that applied range, treating capacity are large, low cost and other advantages, but also there is obvious shortcoming to treatment of dyeing and printing: microorganism there are certain requirements conditions such as nutriment, pH value, temperature, be difficult to adapt to the feature that dyeing waste water variation water quality is large, dye quantity is various, toxicity is high, and it is larger to there is floor space, not easily purification thoroughly, and colourity and COD concentration is the shortcoming such as up to standard not easily.

Summary of the invention

The technical problem that the present invention mainly solves: in current conventional process dyeing waste water, processing cost is higher, be difficult to regeneration, easy introducing impurity causes secondary pollution, and the present invention uses reed rod for main body, then carries out modification charing to it, then bio-modification is being carried out, be aided with additive to mix, the namely obtained renewable use of one, the decolorizing printing and dyeing waste water agent that cost of manufacture is low.

In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:

(1) fresh reed rod is got, with water by clean for its surface clean, use stalk crasher to be pulverized and grow into 2 ~ 4cm reed rod particle, then put it in container, add the sodium chloride solution immersion reed rod particle that mass fraction is 60% wherein, the mass fraction adding sodium chloride solution volume 10 ~ 20% subsequently is again the hydrogen peroxide of 30%, stirs;

(2) after above-mentioned stirring, container is heated, until the liquid in container becomes 60 ~ 70% of primary liquid volume, then room temperature is naturally cooled to, filter to obtain the reed rod particle of preliminary treatment, again reed rod being put into air-dry machine carries out air-dry, subsequently by the reed rod after air-dry and 80 ~ 100 object gangues, mixes by quality 6:1;

(3) above-mentioned mix after, the mixture of gained is put into extruder and is squeezed into bulk, then block is put into retort, at temperature is 200 ~ 230 DEG C, pre-charing 30 ~ 50min, subsequently the block after charing is put into pulverizer to pulverize, sieve to obtain 60 ~ 80 order particles;

(4) particle of above-mentioned gained is mixed by solid-to-liquid ratio 1:2 with bio-modification liquid, put into fermentation vat, add sucrose fatty ester wherein, addition is 2 ~ 4% of particle quality, mix, sealing and fermenting 10 ~ 15 days, it is 30 ~ 40 DEG C that temperature controls, after fermentation ends, remove zymotic fluid, the fermentate of gained is put into air-dry machine air-dry, then put into pulverizer and pulverize, sieve to obtain 30 ~ 80 order particles, can obtain decolorizing printing and dyeing waste water agent.

In described step (4), the preparation method of bio-modification liquid is:

(1) get pond bottom mud and carry out natural drying, when its water content is 10 ~ 30%, put it in fermentation vat, respectively to the sweet potato wherein adding pond bottom sludge quality 10 ~ 15%, the ciliate desert-grass of pond bottom quality 16 ~ 20% and the mustard seed of pond bottom sludge quality 10 ~ 12%, stir;

(2) by quality hundred parts of ratios, get the agar choosing 20 ~ 65 parts, the beef extract of 5 ~ 15 parts, the mass fraction of 15 ~ 20 parts is the glucose solution of 10% and the distilled water of 20 ~ 45 parts, stir, obtain nutrients, the nutrients of 5 ~ 10% of pond bottom sludge quality is added in fermentation vat, stir, temperature is kept to be 30 ~ 35 DEG C, sealing and fermenting 10 ~ 20 days, then in fermentation vat, dyeing waste water is added, sealing stirring 30 ~ 50min, after leaving standstill 5 ~ 7h again, filter, if when the colourity of measured filtered fluid is less than 80 times, stop adding dyeing waste water, otherwise after to be filtered, dyeing waste water is added again in fermentation vat, circulation like this, until the colourity of filtered fluid is less than 80 times, the addition of dyeing waste water is the equal-volume of distilled water,

(3) after above-mentioned filtered fluid reaches standard, the isopyknic distilled water of filtered fluid is added, with 200 ~ 300r/min to fermentation vat, after stirring 20 ~ 30min, the mixture in fermentation vat is put into centrifuge, carries out centrifugation, collect supernatant, bio-modification liquid can be obtained.

Application process of the present invention: after the decolorizer running water got obtained by 1 ~ 3mg the present invention dilutes 10 times, put into the pending Sudan red dyes waste water of 1 pending ~ 2L at once, and stir 30 ~ 40s with agitator with the speed of 300 ~ 350r/min, leave standstill 10 ~ 15min afterwards, 3 ~ 5min is stirred with the speed of 80 ~ 100r/min again with agitator, finally detect colourity and COD with detector, testing result is that colourity is from 50 ~ 60mg/L, be reduced to 10 ~ 12mg/L, COD has been reduced to 0.3 ~ 0.45mg/L from 15 ~ 25mg/L, reach waste water from dyestuff discharge standard.

The invention has the beneficial effects as follows:

(1) cost of manufacture of the present invention is low, is easy to operation;

(2) decolorizer that the present invention is made, decolorizing effect reaches more than 80%;

(3) decolorizer that the present invention is made is reusable, is easy to activation.

Detailed description of the invention

Get fresh reed rod, with water by clean for its surface clean, use stalk crasher to be pulverized and grow into 2 ~ 4cm reed rod particle, then put it in container, add the sodium chloride solution immersion reed rod particle that mass fraction is 60% wherein, the mass fraction adding sodium chloride solution volume 10 ~ 20% subsequently is again the hydrogen peroxide of 30%, stirs; After above-mentioned stirring, container is heated, until the liquid in container becomes 60 ~ 70% of primary liquid volume, then room temperature is naturally cooled to, filter to obtain the reed rod particle of preliminary treatment, again reed rod being put into air-dry machine carries out air-dry, subsequently by the reed rod after air-dry and 80 ~ 100 object gangues, mixes by quality 6:1; Above-mentioned mix after, the mixture of gained is put into extruder and is squeezed into bulk, then block is put into retort, at temperature is 200 ~ 230 DEG C, pre-charing 30 ~ 50min, subsequently the block after charing is put into pulverizer and pulverize, sieve to obtain 60 ~ 80 order particles; The particle of above-mentioned gained is mixed by solid-to-liquid ratio 1:2 with bio-modification liquid, puts into fermentation vat, add sucrose fatty ester wherein, addition is 2 ~ 4% of particle quality, mix, sealing and fermenting 10 ~ 15 days, it is 30 ~ 40 DEG C that temperature controls, after fermentation ends, remove zymotic fluid, the fermentate of gained is put into air-dry machine air-dry, then put into pulverizer and pulverize, sieve to obtain 30 ~ 80 order particles, can obtain decolorizing printing and dyeing waste water agent.

The preparation method of described bio-modification liquid is: get pond bottom mud and carry out natural drying, when its water content is 10 ~ 30%, put it in fermentation vat, respectively to the sweet potato wherein adding pond bottom sludge quality 10 ~ 15%, the ciliate desert-grass of pond bottom quality 16 ~ 20% and the mustard seed of pond bottom sludge quality 10 ~ 12%, stir, by quality hundred parts of ratios, get the agar choosing 20 ~ 65 parts, the beef extract of 5 ~ 15 parts, the mass fraction of 15 ~ 20 parts is the glucose solution of 10% and the distilled water of 20 ~ 45 parts, stir, obtain nutrients, the nutrients of 5 ~ 10% of pond bottom sludge quality is added in fermentation vat, stir, temperature is kept to be 30 ~ 35 DEG C, sealing and fermenting 10 ~ 20 days, then in fermentation vat, dyeing waste water is added, sealing stirring 30 ~ 50min, after leaving standstill 5 ~ 7h again, filter, if when the colourity of measured filtered fluid is less than 80 times, stop adding dyeing waste water, otherwise after to be filtered, dyeing waste water is added again in fermentation vat, circulation like this, until the colourity of filtered fluid is less than 80 times, the addition of dyeing waste water is the equal-volume of distilled water, after above-mentioned filtered fluid reaches standard, add the isopyknic distilled water of filtered fluid, with 200 ~ 300r/min to fermentation vat, after stirring 20 ~ 30min, the mixture in fermentation vat is put into centrifuge, carries out centrifugation, collect supernatant, bio-modification liquid can be obtained.

Example 1

Get fresh reed rod, with water by clean for its surface clean, use stalk crasher to be pulverized and grow into 3cm reed rod particle, then put it in container, add the sodium chloride solution immersion reed rod particle that mass fraction is 60% wherein, the mass fraction adding sodium chloride solution volume 15% subsequently is again the hydrogen peroxide of 30%, stirs; After above-mentioned stirring, container is heated, until the liquid in container becomes 65% of primary liquid volume, then room temperature is naturally cooled to, filter to obtain the reed rod particle of preliminary treatment, again reed rod being put into air-dry machine carries out air-dry, subsequently by the reed rod after air-dry and 90 object gangues, mixes by quality 6:1; Above-mentioned mix after, the mixture of gained is put into extruder and is squeezed into bulk, then block is put into retort, at temperature is 220 DEG C, pre-charing 40min, subsequently the block after charing is put into pulverizer and pulverize, sieve to obtain 70 order particles; The particle of above-mentioned gained is mixed by solid-to-liquid ratio 1:2 with bio-modification liquid, puts into fermentation vat, add sucrose fatty ester wherein, addition is 3% of particle quality, mix, sealing and fermenting 12 days, it is 32 DEG C that temperature controls, after fermentation ends, remove zymotic fluid, the fermentate of gained is put into air-dry machine air-dry, then put into pulverizer and pulverize, sieve to obtain 40 order particles, can obtain decolorizing printing and dyeing waste water agent.

The preparation method of described bio-modification liquid is: get pond bottom mud and carry out natural drying, when its water content is 20%, put it in fermentation vat, respectively to the sweet potato wherein adding pond bottom sludge quality 12%, the ciliate desert-grass of pond bottom quality 20% and the mustard seed of pond bottom sludge quality 11%, stir, by quality hundred parts of ratios, get the agar choosing 65 parts, the beef extract of 5 parts, the mass fraction of 15 parts is the glucose solution of 10% and the distilled water of 20 parts, stir, obtain nutrients, the nutrients of 8% of pond bottom sludge quality is added in fermentation vat, stir, temperature is kept to be 31 DEG C, sealing and fermenting 15 days, then in fermentation vat, dyeing waste water is added, 40min is stirred in sealing, after leaving standstill 5h again, filter, if when the colourity of measured filtered fluid is less than 80 times, stop adding dyeing waste water, otherwise after to be filtered, dyeing waste water is added again in fermentation vat, circulation like this, until the colourity of filtered fluid is less than 80 times, the addition of dyeing waste water is the equal-volume of distilled water, after above-mentioned filtered fluid reaches standard, add the isopyknic distilled water of filtered fluid to fermentation vat, with 250r/min, after stirring 25min, the mixture in fermentation vat is put into centrifuge, carries out centrifugation, collect supernatant, bio-modification liquid can be obtained.

After the decolorizer running water got obtained by 1mg the present invention dilutes 10 times, put into the pending Sudan red dyes waste water of pending 1L at once, and stir 30s with agitator with the speed of 300r/min, leave standstill 10min afterwards, then stir 3min with agitator with the speed of 80r/min, finally detect colourity and COD with detector, testing result is that colourity is from 50mg/L, be reduced to 10mg/L, COD and be reduced to 0.3mg/L from 15mg/L, reach waste water from dyestuff discharge standard.

Example 2

Get fresh reed rod, with water by clean for its surface clean, use stalk crasher to be pulverized and grow into 4cm reed rod particle, then put it in container, add the sodium chloride solution immersion reed rod particle that mass fraction is 60% wherein, the mass fraction adding sodium chloride solution volume 20% subsequently is again the hydrogen peroxide of 30%, stirs; After above-mentioned stirring, container is heated, until the liquid in container becomes 70% of primary liquid volume, then room temperature is naturally cooled to, filter to obtain the reed rod particle of preliminary treatment, again reed rod being put into air-dry machine carries out air-dry, subsequently by the reed rod after air-dry and 100 object gangues, mixes by quality 6:1; Above-mentioned mix after, the mixture of gained is put into extruder and is squeezed into bulk, then block is put into retort, at temperature is 230 DEG C, pre-charing 50min, subsequently the block after charing is put into pulverizer and pulverize, sieve to obtain 80 order particles; The particle of above-mentioned gained is mixed by solid-to-liquid ratio 1:2 with bio-modification liquid, puts into fermentation vat, add sucrose fatty ester wherein, addition is 4% of particle quality, mix, sealing and fermenting 15 days, it is 40 DEG C that temperature controls, after fermentation ends, remove zymotic fluid, the fermentate of gained is put into air-dry machine air-dry, then put into pulverizer and pulverize, sieve to obtain 80 order particles, can obtain decolorizing printing and dyeing waste water agent.

The preparation method of described bio-modification liquid is: get pond bottom mud and carry out natural drying, when its water content is 30%, put it in fermentation vat, respectively to the sweet potato wherein adding pond bottom sludge quality 15%, the ciliate desert-grass of pond bottom quality 16% and the mustard seed of pond bottom sludge quality 12%, stir, by quality hundred parts of ratios, get the agar choosing 20 parts, the beef extract of 15 parts, the mass fraction of 20 parts is the glucose solution of 10% and the distilled water of 45 parts, stir, obtain nutrients, the nutrients of 10% of pond bottom sludge quality is added in fermentation vat, stir, temperature is kept to be 35 DEG C, sealing and fermenting 20 days, then in fermentation vat, dyeing waste water is added, 50min is stirred in sealing, after leaving standstill 7h again, filter, if when the colourity of measured filtered fluid is less than 80 times, stop adding dyeing waste water, otherwise after to be filtered, dyeing waste water is added again in fermentation vat, circulation like this, until the colourity of filtered fluid is less than 80 times, the addition of dyeing waste water is the equal-volume of distilled water, after above-mentioned filtered fluid reaches standard, add the isopyknic distilled water of filtered fluid to fermentation vat, with 300r/min, after stirring 30min, the mixture in fermentation vat is put into centrifuge, carries out centrifugation, collect supernatant, bio-modification liquid can be obtained.

After the decolorizer running water got obtained by 3mg the present invention dilutes 10 times, put into the pending Sudan red dyes waste water of pending 2L at once, and stir 40s with agitator with the speed of 350r/min, leave standstill 15min afterwards, then stir 5min with agitator with the speed of 100r/min, finally detect colourity and COD with detector, testing result is that colourity is from 60mg/L, be reduced to 12mg/L, COD and be reduced to 0.45mg/L from 25mg/L, reach waste water from dyestuff discharge standard.

Example 3

Get fresh reed rod, with water by clean for its surface clean, use stalk crasher to be pulverized and grow into 2m reed rod particle, then put it in container, add the sodium chloride solution immersion reed rod particle that mass fraction is 60% wherein, the mass fraction adding sodium chloride solution volume 10% subsequently is again the hydrogen peroxide of 30%, stirs; After above-mentioned stirring, container is heated, until the liquid in container becomes 60% of primary liquid volume, then room temperature is naturally cooled to, filter to obtain the reed rod particle of preliminary treatment, again reed rod being put into air-dry machine carries out air-dry, subsequently by the reed rod after air-dry and 80 object gangues, mixes by quality 6:1; Above-mentioned mix after, the mixture of gained is put into extruder and is squeezed into bulk, then block is put into retort, at temperature is 200 DEG C, pre-charing 30min, subsequently the block after charing is put into pulverizer and pulverize, sieve to obtain 60 order particles; The particle of above-mentioned gained is mixed by solid-to-liquid ratio 1:2 with bio-modification liquid, puts into fermentation vat, add sucrose fatty ester wherein, addition is 2% of particle quality, mix, sealing and fermenting 10 days, it is 30 DEG C that temperature controls, after fermentation ends, remove zymotic fluid, the fermentate of gained is put into air-dry machine air-dry, then put into pulverizer and pulverize, sieve to obtain 30 order particles, can obtain decolorizing printing and dyeing waste water agent.

The preparation method of described bio-modification liquid is: get pond bottom mud and carry out natural drying, when its water content is 10%, put it in fermentation vat, respectively to the sweet potato wherein adding pond bottom sludge quality 10%, the ciliate desert-grass of pond bottom quality 16 ~ 20% and the mustard seed of pond bottom sludge quality 10%, stir, by quality hundred parts of ratios, get the agar choosing 65 parts, the beef extract of 15 parts, the mass fraction of 20 parts is the glucose solution of 10% and the distilled water of 20 parts, stir, obtain nutrients, the nutrients of 5% of pond bottom sludge quality is added in fermentation vat, stir, temperature is kept to be 30 DEG C, sealing and fermenting 10 days, then in fermentation vat, dyeing waste water is added, 30min is stirred in sealing, after leaving standstill 5h again, filter, if when the colourity of measured filtered fluid is less than 80 times, stop adding dyeing waste water, otherwise after to be filtered, dyeing waste water is added again in fermentation vat, circulation like this, until the colourity of filtered fluid is less than 80 times, the addition of dyeing waste water is the equal-volume of distilled water, after above-mentioned filtered fluid reaches standard, add the isopyknic distilled water of filtered fluid to fermentation vat, with 200r/min, after stirring 20min, the mixture in fermentation vat is put into centrifuge, carries out centrifugation, collect supernatant, bio-modification liquid can be obtained.

After the decolorizer running water got obtained by 2mg the present invention dilutes 10 times, put into the pending Sudan red dyes waste water of pending 1L at once, and stir 35s with agitator with the speed of 320r/min, leave standstill 12min afterwards, then stir 4min with agitator with the speed of 90r/min, finally detect colourity and COD with detector, testing result is that colourity is from 60mg/L, be reduced to 10mg/L, COD and be reduced to 0.4mg/L from 20mg/L, reach waste water from dyestuff discharge standard.

Claims (2)

1. modification reed rod prepares a method for decolorizing printing and dyeing waste water agent, it is characterized in that concrete treatment step is:

(1) fresh reed rod is got, with water by clean for its surface clean, use stalk crasher to be pulverized and grow into 2 ~ 4cm reed rod particle, then put it in container, add the sodium chloride solution immersion reed rod particle that mass fraction is 60% wherein, the mass fraction adding sodium chloride solution volume 10 ~ 20% subsequently is again the hydrogen peroxide of 30%, stirs;

(2) after above-mentioned stirring, container is heated, until the liquid in container becomes 60 ~ 70% of primary liquid volume, then room temperature is naturally cooled to, filter to obtain the reed rod particle of preliminary treatment, again reed rod being put into air-dry machine carries out air-dry, subsequently by the reed rod after air-dry and 80 ~ 100 object gangues, mixes by quality 6:1;

(3) above-mentioned mix after, the mixture of gained is put into extruder and is squeezed into bulk, then block is put into retort, at temperature is 200 ~ 230 DEG C, pre-charing 30 ~ 50min, subsequently the block after charing is put into pulverizer to pulverize, sieve to obtain 60 ~ 80 order particles;

(4) particle of above-mentioned gained is mixed by solid-to-liquid ratio 1:2 with bio-modification liquid, put into fermentation vat, add sucrose fatty ester wherein, addition is 2 ~ 4% of particle quality, mix, sealing and fermenting 10 ~ 15 days, it is 30 ~ 40 DEG C that temperature controls, after fermentation ends, remove zymotic fluid, the fermentate of gained is put into air-dry machine air-dry, then put into pulverizer and pulverize, sieve to obtain 30 ~ 80 order particles, can obtain decolorizing printing and dyeing waste water agent.

2. a kind of modification reed rod according to claim 1 prepares the method for decolorizing printing and dyeing waste water agent, it is characterized in that: in described step (4), the preparation method of bio-modification liquid is:

(1) get pond bottom mud and carry out natural drying, when its water content is 10 ~ 30%, put it in fermentation vat, respectively to the sweet potato wherein adding pond bottom sludge quality 10 ~ 15%, the ciliate desert-grass of pond bottom quality 16 ~ 20% and the mustard seed of pond bottom sludge quality 10 ~ 12%, stir;

(2) by quality hundred parts of ratios, get the agar choosing 20 ~ 65 parts, the beef extract of 5 ~ 15 parts, the mass fraction of 15 ~ 20 parts is the glucose solution of 10% and the distilled water of 20 ~ 45 parts, stir, obtain nutrients, the nutrients of 5 ~ 10% of pond bottom sludge quality is added in fermentation vat, stir, temperature is kept to be 30 ~ 35 DEG C, sealing and fermenting 10 ~ 20 days, then in fermentation vat, dyeing waste water is added, sealing stirring 30 ~ 50min, after leaving standstill 5 ~ 7h again, filter, if when the colourity of measured filtered fluid is less than 80 times, stop adding dyeing waste water, otherwise after to be filtered, dyeing waste water is added again in fermentation vat, circulation like this, until the colourity of filtered fluid is less than 80 times, the addition of dyeing waste water is the equal-volume of distilled water,

(3) after above-mentioned filtered fluid reaches standard, the isopyknic distilled water of filtered fluid is added, with 200 ~ 300r/min to fermentation vat, after stirring 20 ~ 30min, the mixture in fermentation vat is put into centrifuge, carries out centrifugation, collect supernatant, bio-modification liquid can be obtained.