CN105535026A - Application of lumbricus in preparing anti-radiation product - Google Patents
Application of lumbricus in preparing anti-radiation product Download PDFInfo
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- CN105535026A CN105535026A CN201610009611.1A CN201610009611A CN105535026A CN 105535026 A CN105535026 A CN 105535026A CN 201610009611 A CN201610009611 A CN 201610009611A CN 105535026 A CN105535026 A CN 105535026A
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- pheretima
- radiation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/62—Leeches; Worms, e.g. cestodes, tapeworms, nematodes, roundworms, earth worms, ascarids, filarias, hookworms, trichinella or taenia
Abstract
The invention relates to the technical field of medicine and food, in particular to application of lumbricus in preparing an anti-radiation product. Though a lot of experimental research for screening medicine with the anti-radiation effect, it is found that if lumbricus and an extract thereof are fed to a mouse to be radiated in advance, the survival rate of the mouse can be improved, cell apoptosis is reduced, the lesion degree of the tissue of the mouse is lowered, and it shows that the lumbricus and the extract thereof have the anti-radiation effect.
Description
Technical field
The present invention relates to medicine food technical field, specifically, is the novelty teabag of Pheretima and extract thereof, is more particularly that Pheretima and extract thereof are preparing the new opplication in anti-radiation product.
Background technology
Radiation is comparatively large to the harm of organism, can directly act on DNA, protein and enzyme, cause ionization to excite chemical bond rupture, and molecule degeneration and cellularity are destroyed; Also can act on hydrone in body, make it ionization occur and excite, produce free radical, indirectly make histiocyte degeneration, necrosis, so that organism metabolic disorder, cause a series of pathological changes such as regulatory function obstacle of immunity, nerve and hormonal system.The development of science and technology and the day by day extensive of radiation application, particularly medically accept the patient of diagnosis and chemotherapy, be all be directly exposed under radiation, radiation protection is further difficult, and radiation damage also aggravates.Although to the development of antiradiation drug through the unremitting effort of decades, found some active drugs, present spendable antiradiation drug is less, comprise estrogen and sulfur-containing compound, but side effect is more.Sulfur-bearing radioprotectant shows good preventive effect, but its toxic and side effects is obvious, only just demonstrates radioprotective activity when reaching toxic dose.Therefore, radioprotective research steering natural materials comes.Chinese traditional herbs and some nourishing foods of China become object of study, achieve larger progress.
Pheretima is as conventional animal medical material, common, be easy to get, in the existing thousands of years history of China's application.The drying of the blind blind earthworm Pheretimapectinifera (Michaelsen) far away of Pheretima Shi Ju earthworm section animal Pheretima aspergillum Pheretimaaspergillum (E.Perrier), power simulation Pheretimaguillelmi (Michaelsen), popular Pheretimatschiliensis Pheretimavulgaris (Chen) or comb is all.Front one is practised and is claimed " LUMBRICUS ", practises title " Shanghai Pheretima " for latter three kinds.Pheretima begins to be loaded in Shennong's Herbal, and property is salty, cold, returns liver, spleen, urinary bladder channel, has suppressing the hyperactive liver to relieve the wind syndrome, heat clearing away relieving convulsion, dredging collateral, relievings asthma, effect of diuresis, for unconsciousness due to high fever, infantile convulsion is twitched, arthralgia, numb limbs and tense tendons, hemiplegia, dyspnea and cough due to lung-heat, oliguria edema, the diseases such as hypertension.In Pheretima, various active composition makes it have multiple pharmacological effect, as anti-fibrinolytic and anticoagulant active, blood pressure lowering, relieving asthma, other effects such as fibrosis, promotion CO2 laser weld and regeneration.But yet there are no report about Pheretima and extract thereof preparing the new opplication in anti-radiation product.
Summary of the invention
Pheretima and extract thereof is the object of the present invention is to provide to prepare the new opplication in anti-radiation product.In the drug test research of the present invention by a large amount of screening radiation resistance, find that Pheretima and extract thereof have radiation resistance.Pheretima and extract thereof are given the mice that will accept radiation by the present invention's display in advance, can improve the survival rate of mice, reduce apoptosis, improve the pathological changes of radiation murine small intestine, show that Pheretima and extract thereof have radiation resistance.
A first aspect of the present invention, provides Pheretima preparing the application in anti-radiation product.
Described radiation is radioprotective or ionizing radiation, preferred gamma Rays.
Described Pheretima refers to each kind of Lumbricus with medication value, comprise power simulation Pheretimaguillelmi (Michaelsen), popular Pheretimatschiliensis P.vulgarisChen, the blind Pheretimatschiliensis P.pectiniferaMichaelsen of comb, Pheretima aspergillum P.aspergillumE.Perrier, Eisenia foetida EiseniafoetidaSavigny, Hubei Pheretimatschiliensis P.hupeiensisMichaelsen etc.Preferred power simulation Pheretimaguillelmi (Michaelsen).
Described anti-radiation product is medicine or the health product of prevention or treatment radiation damage.
Preferably, described medicine or health product are using Pheretima or Pheretima extract as sole active agent.
Described Pheretima extract is Pheretima powder, or aqueous extract of earthworm and ethanol extract.
The preparation method of described Pheretima powder: fresh Pheretima, adds 2.5 times of water, and after homogenate, solution extracts 8 hours in 4 DEG C, and centrifugal 1 time, supernatant adds 95% ethanol to final concentration 75%, precipitate more than 4 hours, removes supernatant centrifugal 1 time, precipitation collection, spraying dry and get final product.
The preparation method of aqueous extract of earthworm and ethanol extract: fresh Pheretima, add 2 times of water, homogenate, 4 DEG C are extracted 8 hours, centrifugal 1 time, supernatant adds 95% ethanol to final concentration 75%, precipitation, centrifugally removes supernatant, and namely collecting precipitation lyophilizing obtains aqueous extract of earthworm, supernatant less than 60 DEG C rotary evaporations concentrate, and lyophilizing obtains Lumbricus ethanol extract.
Described pharmaceutical dosage form comprise in tablet, capsule, granule, suspensoid, Emulsion, solution, syrup, unguentum, patch or injection etc. one or more, take one or more route of administration in oral, external, injection (comprise in intravenous injection, intravenous drip, intramuscular injection or subcutaneous injection etc. one or more) etc. to carry out the prevention of radiation and directly related disease thereof, protection or treatment.
A second aspect of the present invention, provides a kind of antiradiation drug or health product, and described medicine or health product are using Pheretima or Pheretima extract as sole active agent.
Present invention finds the survival rate that Pheretima and extract thereof can improve mice after gamma Rays, reduce apoptosis, improve the lesion degree of radiation murine tissue, illustrate that Pheretima and extract thereof have radiation resistance.
Accompanying drawing explanation
Fig. 1 is that Pheretima and extract thereof are on the impact (* P<0.05, * * P<0.01 medicine group vs. model group) of mouse survival rate.
Detailed description of the invention
Below in conjunction with embodiment, detailed description of the invention provided by the invention is elaborated.The embodiment of the following stated is to set forth the present invention better, is not for limiting the scope of the invention.
The preparation of embodiment 1. Pheretima extract
1.1 experiment material
Pheretima powder: fresh Pheretima 500.1g, adds 2.5 times of water, and after homogenate, solution extracts 8 hours in 4 DEG C, and centrifugal 1 time, supernatant adds 95% ethanol to final concentration 75%, precipitate more than 4 hours, removes supernatant centrifugal 1 time, precipitation collection, spraying dry and get final product.
Aqueous extract of earthworm and ethanol extract: fresh Pheretima 500.1g, add 2 times of water, homogenate, 4 DEG C are extracted 8 hours, centrifugal 1 time, supernatant adds 95% ethanol to final concentration 75%, precipitation, centrifugally removes supernatant, and namely collecting precipitation lyophilizing obtains aqueous extract of earthworm, supernatant less than 60 DEG C rotary evaporations concentrate, and lyophilizing obtains Lumbricus ethanol extract.
The protective effect that embodiment 2. Pheretima and extract thereof cause mice to damage to radiation
2.1 experiment material
Fresh Pheretima, gathers from Pheretima cultivation base, Shanghai, Shanghai, is accredited as Shanghai Pheretima kind power simulation Pheretimaguillelmi (Michaelsen) through Zhang Hanming professor (pharmaceutical college of The 2nd Army Medical College).
C57 mice purchased from Shanghai Slac Experimental Animal Co., Ltd., ticket number SCXK (Shanghai) 2012-0002.
2.2 experimental technique
2.2.1 Pheretima and extract thereof cause the impact of mouse survival rate to radiation
C57 mouse stomach gives the Pheretima powder (150mg/kg/d) of various dose and water extract (50mg/kg/d) thereof and ethanol extract (100mg/kg/d) two weeks, then with 9Gy
60co-gamma Rays, after radiation, mice continues gastric infusion, observes the survival rate of mice in 30 days after radiation.
2.3 statistical procedures
Experimental data is all with mean ± standard deviation
represent, between each group, data compare and use SPSS17.0 software kit, and significance analysis is carried out in employing one factor analysis of variance (ANOVA).
2.4 experimental result
2.4.1 on the impact of survival rate after the radiation of C57 mice
Pheretima and extract thereof significantly improve the survival rate of mice after the radiation of 9Gy fatal dose.As shown in Figure 1, the life cycle of radiation patterns group mice is not all more than 7 days; Compare with model group, the survival rate of Pheretima and extract group thereof is significantly improved, and mice is lived forever and live by the 16th day most.Experimental result explanation; Pheretima and extract thereof have radiation resistance to organism; Pheretima and extract thereof can be made the agent of organism Radiological Defense, and with feasible dosage and dosage form, organism be intervened, to reach the object of the organism under protection gamma Rays.
Below the preferred embodiment of the invention is illustrated, but the invention is not limited to described embodiment, those of ordinary skill in the art also can make all equivalent modification or replacement under the prerequisite without prejudice to the invention spirit, and these equivalent modification or replacement are all included in the application's claim limited range.
Claims (8)
1. Pheretima is preparing the application in anti-radiation product.
2. Pheretima according to claim 1 is preparing the application in anti-radiation product, it is characterized in that, described anti-radiation product is medicine or the health product of prevention or treatment radiation damage.
3. Pheretima according to claim 1 is preparing the application in anti-radiation product, it is characterized in that, described radiation is radioprotective or ionizing radiation.
4. Pheretima according to claim 1 is preparing the application in anti-radiation product, it is characterized in that, described radiation is gamma Rays.
5. Pheretima according to claim 1 is preparing the application in anti-radiation product, it is characterized in that, described Pheretima is selected from power simulation Pheretimaguillelmi (Michaelsen), popular Pheretimatschiliensis P.vulgarisChen, the blind Pheretimatschiliensis P.pectiniferaMichaelsen of comb, Pheretima aspergillum P.aspergillumE.Perrier, Eisenia foetida EiseniafoetidaSavigny, one or more in the Pheretimatschiliensis P.hupeiensisMichaelsen of Hubei.
6. Pheretima according to claim 2 is preparing the application in anti-radiation product, it is characterized in that, described medicine or health product are using Pheretima or Pheretima extract as sole active agent.
7. Pheretima according to claim 2 is preparing the application in anti-radiation product, it is characterized in that, described pharmaceutical dosage form is tablet, capsule, granule, suspensoid, Emulsion, solution, syrup, unguentum, patch or injection.
8. antiradiation drug or health product, is characterized in that, described medicine or health product are using Pheretima or Pheretima extract as sole active agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610009611.1A CN105535026A (en) | 2016-01-08 | 2016-01-08 | Application of lumbricus in preparing anti-radiation product |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610009611.1A CN105535026A (en) | 2016-01-08 | 2016-01-08 | Application of lumbricus in preparing anti-radiation product |
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| CN105535026A true CN105535026A (en) | 2016-05-04 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2451324A1 (en) * | 2001-06-28 | 2003-01-09 | Rongxiang Xu | Physiological tissue repair and functional organ regeneration by cultivation of regenerative stem cells in vivo and in situ |
| CN1606929A (en) * | 2003-10-16 | 2005-04-20 | 吴永胜 | Highly active multifunctional products series of earthworm and its production method |
| CN1836683A (en) * | 2005-03-21 | 2006-09-27 | 兰州殿稳医疗制品有限公司 | Medicine for treating tumour and its preparing method |
| CN1857350A (en) * | 2006-03-27 | 2006-11-08 | 珠海博康药业有限公司 | Immunological function strengthening medicine and health product and their preparing method |
| CN105125960A (en) * | 2015-09-21 | 2015-12-09 | 崇州市地龙海龙生物制品开发研究所 | Method for preparing tablets capable of clearing heat to remove toxin |
-
2016
- 2016-01-08 CN CN201610009611.1A patent/CN105535026A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2451324A1 (en) * | 2001-06-28 | 2003-01-09 | Rongxiang Xu | Physiological tissue repair and functional organ regeneration by cultivation of regenerative stem cells in vivo and in situ |
| CN1606929A (en) * | 2003-10-16 | 2005-04-20 | 吴永胜 | Highly active multifunctional products series of earthworm and its production method |
| CN1836683A (en) * | 2005-03-21 | 2006-09-27 | 兰州殿稳医疗制品有限公司 | Medicine for treating tumour and its preparing method |
| CN1857350A (en) * | 2006-03-27 | 2006-11-08 | 珠海博康药业有限公司 | Immunological function strengthening medicine and health product and their preparing method |
| CN105125960A (en) * | 2015-09-21 | 2015-12-09 | 崇州市地龙海龙生物制品开发研究所 | Method for preparing tablets capable of clearing heat to remove toxin |
Non-Patent Citations (1)
| Title |
|---|
| 傅炜昕 等: "免疫活性地龙肽的制备及其对小鼠巨噬细胞活性的影响", 《微生物学杂志》 * |
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Application publication date: 20160504 |
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