CN105525014A - Universal PCR detection primers and detection method for vertebrate-derived ingredients - Google Patents
Universal PCR detection primers and detection method for vertebrate-derived ingredients Download PDFInfo
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- CN105525014A CN105525014A CN201610061006.9A CN201610061006A CN105525014A CN 105525014 A CN105525014 A CN 105525014A CN 201610061006 A CN201610061006 A CN 201610061006A CN 105525014 A CN105525014 A CN 105525014A
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- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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Abstract
The invention discloses universal PCR detection primers and a detection method for vertebrate-derived ingredients. The sequences of the primer pair are as shown in F-primer: 5'-TGTTTACCAAAAACATCACCTCCA-3', and R-primer: 5'-AGTTAAAGCTCCATAGGGTCT-3'. According to the invention, the pair of universal primers is designed in a highly conserved domain in accordance with various vertebrate mitochondrion DNA (mtDNA) sequence revealed in GenBank, and the universal PCR detection method is established; experimental results show that the primers are relatively strong in university, and effective amplification on all collected samples is achieved; and in accordance with sequencing results, the effective identification of species is achieved.
Description
Technical field
The invention belongs to field of molecular detection, the universal PC R being specifically related to a kind of vertebrates derived component detects primer and detection method.
Background technology
In the ring of light of modern civilization society, the mankind order about the behavior of illegally catching and killing all kinds of animal by interests and remain incessant after repeated prohibition, and the eubiosis in havoc.The phenomenon of palming off rare animal goods Chinese medicinal materials and hide in commodity transaction is also growing on and on, the grievous injury rights and interests of human consumer.From the angle of Field of Animal Epidemic Disease Control, China has also put into effect multi-section relevant laws and regulations to the cultivation of animal class agricultural byproducts, production and the course of processing, calendar year 2001 No. 7 file is sent out in the agriculture and animal husbandry of putting into effect as National agricultural portion, just defines for the prevention and control of mad cow disease and sheep itch and forbids adding in ruminant feed and use animal feedstuff.Variously animal, animal organ or tissue, the production behavior of animals products, usage behavior, trading activity and illegal act is related to for above-mentioned; all need its source of species is carried out to science, identifies accurately; namely this is the important evidence of law enforcement, is also the core means of carrying out protection of animal, specification production process and safeguarding consumer rights.
According to the classification of " Chinese animal classification code part 1 vertebrates " (GB/T15628.1-2009), vertebrate comprises without jaw guiding principle, amphibia, Mammalia, Aves, net-rope and reptilia, this wherein contain in our daily life touch nearly all domestic animal, poultry, flying bird, fish and wildlife.Because vertebrate biotic component is complicated, the chemical composition of relevant animal goods is various, and containing a large amount of similar components such as protein, fat, use general sense organ and physics and chemistry discrimination method effect all not satisfactory, the correlation techniques such as existing Infrared spectroscopy also cannot very accurately or easily be identified due to the deficiency of self.Round pcr with its comparatively high specific and sensitivity generally adopted both at home and abroad, but existing detection method is confined to the method for one or more animal derived materials mostly, and the research for the general detection of vertebrate, authenticate technology is also comparatively rare.
Summary of the invention
For deficiency existing in prior art, a kind of universal PC R of vertebrates derived component is the object of the present invention is to provide to detect primer and detection method.
The technical solution used in the present invention is:
For detecting a general PCR primer pair for vertebrates derived component, it designs according to the consensus sequence of vertebrate Mitochondrial DNA.
As preferably, the nucleotide sequence of described primer pair is as follows:
F-primer：5’-TGTTTACCAAAAACATCACCTCCA-3’(SEQIDNO.1)；
R-primer：5’-AGTTAAAGCTCCATAGGGTCT-3’(SEQIDNO.2)。
A PCR detection method for vertebrates derived component, comprises the steps:
(1) extract sample DNA as amplification template, vertebrates source property sample DNA is positive control;
(2) pcr amplification is carried out with the primer pair described in claim 1 or 2;
(3) gel electrophoresis is carried out to PCR product, whether contain musk deer derived component according in electrophoresis result judgement sample.
Consisting of of 25 μ LPCR reaction systems: 2 × TaqPCRMastermix12.5 μ L, 10 μm of each 1.0 μ L of ol/L upstream and downstream primer, DNA masterplate 2.0 μ L, moisturizing to 25 μ L.
PCR response procedures is: 94 DEG C of 4min; 94 DEG C of 30s, 58 DEG C of 30s, 72 DEG C of 30s, 35 circulations; 72 DEG C of 5min.
The invention has the beneficial effects as follows:
The present invention is according to multiple vertebrates Mitochondrial DNA (mtDNA) sequence announced in GenBank, devise a pair universal primer in its high conservative region and set up general PCR detection method, experimental result shows this primer highly versatile, achieve the effective amplification to all samples collected, achieve the effective qualification to species in conjunction with sequencing result.
Accompanying drawing explanation
Fig. 1 is vertebrates derived component PCR method versatility test-results (M:DNAMarkerDL2000; N: negative control; B: blank; 1: beef; 2: pork; 3: mutton; 4: chicken; 5: mink skin; 6: deer horn; 7: donkey meat; 8: goose; 9: musk deer hide; 10: fox hide; 11: duck; 12: dog meats; 13: rabbit meat; 14: sheep's horn; 15: Cornu rhinocerotis powder; 16: ivory; 17: mouse meat; 18: hump meat; 19: bear gall; 20: cat hair; 21: horseflesh; 22: Mo Guiyu; 23: seven color brontosaurus fishes; 24: eel; 25: Jin Longyu; 26: silver-colored imperial fish 27: tilapia; 28: saury; 29: salmon; 30: crucian; 31: perch; 32: grass carp; 33: coilia; 34: Scatophagus argus (Linnaeus); 35: small yellow croaker; 36: grey silvery pomfret; 37: eye spot intends lip fish; 38: Yellow catfish; 39: hairtail; 40: strange Butterfly fish; 41: undersea boat fish; 42: brave head shark; 43: the true shark in Maxwell; 44: plough fin lemon shark; 45: Pacific Ocean ratfish; 46: hippocampus does; 47: feather of birds; 48: pigeon meat; 49: frog meat; 50: tortoise);
Fig. 2 is vertebrates derived component PCR method sensitivity test result (M:DNAMarkerDL2000; N: negative control; 1-10: gradient is followed successively by 10
10, 10
9, 10
8, 10
7, 10
6, 10
5, 10
4, 10
3, 10
2, 10
1).
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, but be not limited thereto.
1 materials and methods
1.1 sample
Totally 114 parts, the vertebrate animal tissues sample of laboratory preservation and outsourcing, wherein Mammalia 63 parts, 33 parts, net-rope, Aves 14 parts, amphibia 2 parts, reptilia 2 parts.
Table 1 Mammalia animal sample
| Sample ID | Sample size | Sample ID | Sample size |
| Beef | 18 | Fox hide | 2 |
| Pork | 10 | Horseflesh | 1 |
| Mutton | 10 | Sheep's horn | 1 |
| Mink skin | 4 | Cornu rhinocerotis powder | 1 |
| Deer horn | 3 | Ivory | 1 |
| Donkey meat | 2 | Mouse meat | 1 |
| Rabbit meat | 2 | Hump meat | 1 |
| Dog meats | 2 | Cat hair | 1 |
| Musk deer hide | 2 | Bear gall | 1 |
Table 2 net-rope animal sample
| Sample ID | Sample size | Sample ID | Sample size |
| Devil fish | 4 | Shark fin | 7 |
| Seven color brontosaurus fishes | 2 | Eye spot intends lip fish | 1 |
| Eel | 2 | Yellow catfish | 1 |
| Jin Longyu | 1 | Hairtail | 1 |
| The imperial fish of silver | 1 | Strange Butterfly fish | 1 |
| Tilapia | 1 | Undersea boat fish | 1 |
| Saury | 1 | Ash silvery pomfret | 1 |
| Salmon | 1 | Coilia | 1 |
| Crucian | 1 | Scatophagus argus (Linnaeus) | 1 |
| Perch | 1 | Small yellow croaker | 1 |
| Grass carp | 1 | Hippocampus does | 1 |
Other guiding principle sample of table 3
1.2 main agents
DNA extraction kit E.Z.N.A.TMTissueDNAKit is U.S. OMEGA Products, PCR reaction system TIANGENTaqPCRMastermix is TIANGEN Biotech's product, DNAMarkerDL2000 is TaKaRa product, purchased from precious biotechnology (Dalian) company limited.
1.3 key instrument
9700PCR instrument is U.S. AppliedBiosystems Products.AlphaImagerHP Labworks image acquisition and analysis software is U.S. AlphaInnotech Products.The freezing desk centrifuge of Sigma3-18K miniature high-speed is German Sartorius Products.NanoDropND-1000Spectrophotometer ultraviolet spectrophotometer is U.S. NanoDropTechnologies Products.
The synthesis of 1.4PCR primer
According to announcing vertebrates Mitochondrial DNA (mtDNA) sequence in GenBank; a pair general PCR primer of vertebrates is designed at high conservative region Oligo7.0 after utilizing biological software ClustalX comparison; synthesized by Hui Rui bio tech ltd, Shanghai, primer sequence is in table 4.
Table 4 vertebrates primer sequence
The DNA extraction of 1.5 samples and pcr amplification
Sample DNA is extracted according to DNA extraction kit specification sheets.Sample DNA is carried out pcr amplification as template, and gel electrophoresis is carried out to PCR primer.PCR reaction system is 25 μ L:2 × TaqPCRMastermix12.5 μ L, 10 μm of each 1.0 μ L of ol/L upstream and downstream primer, DNA masterplate 2.0 μ L, moisturizing to 25 μ L.Response procedures: 94 DEG C of 4min; 94 DEG C of 30s, 58 DEG C of 30s, 72 DEG C of 30s, 35 circulations; 72 DEG C of 5min.
The electrophoresis of 1.6PCR product and order-checking
Amplification terminate after in 1% sepharose and 1XTBE buffered soln electrophoresis detection, voltage 100V, 30min.After electrophoresis terminates, the result after electrophoresis is observed with AlphaImagerHP Labworks image acquisition and analysis software, and will the PCR primer of object band be had to carry out cloning and sequencing, use the research tool BasicLocalAlignmentSearchTool (BLAST) of the GenBank database of American National biotechnology center (NCBI) after obtaining sequence
[11]determine source of species.
1.7PCR method versatility is tested
With the DNA of 114 parts of vertebrate animal tissues sample extraction for template, use above-mentioned reaction system and condition to carry out pcr amplification, verify the versatility of the PCR method set up.
1.8PCR method sensitivity test
By PCR primer electrophoresis detection, the PCR primer of object band is had to send Beijing Liuhe Huada Genomics Technology Co., Ltd's Wuhan Company, Guangzhou Branch to carry out order-checking qualification, and using the recombinant plasmid of ox as positive criteria product.Recombinant plasmid ultraviolet spectrophotometer is measured plasmid concentration, is scaled the copy number of goal gene according to Avogadro constant, with 10 times of gradient dilution to 10 copy/μ L, PCR detection is carried out to the positive control of gradient dilution.
2 animal derived sample detection results and analysis
The pcr amplification of 2.1 vertebrates derived component samples and Species estimation result
114 parts of whole Successful amplification of vertebrates derived component sample DNA, product size is about 249bp, and this result conforms to expection.By all PCR primer cloning and sequencings, obtain sequence comparison in GenBank, comparison result is consistent with the animal sample result in known source.
Wherein 50 increment product Gel electrophoresis results are shown in Fig. 1, in GenBank, comparison result is followed successively by ox from 1-50, wild boar, goat, jungle fowl, mink, reinder, donkey, swan goose, horse musk deer, rde fox, mallard, domesticated dog, rabbit, sahilite, white rhinoceros, elephant or African elephant, Rattus norvegicus, two-humped camel, Asian Black Bear, sand dune cat, wild horse, pearl ray, seven color brontosaurus fishes, Cuscuta japonicoa, Jin Longyu, the imperial fish of silver, tilapia, saury, atlantic salmon, crucian, perch, grass carp, coilia, Scatophagus argus (Linnaeus), small yellow croaker, ash silvery pomfret, eye spot intends lip fish, Yellow catfish, hairtail, strange Butterfly fish, dark green Puffer, tiger head shark, the true shark in Maxwell, plough fin lemon shark, Pacific Ocean ratfish, kiss hippocampus, quail, wild pigeon, Mercuric chloride, leopard line Testudo elongata.
Wherein 93 increment product use national standard and industry standard to detect, and result is consistent with general PCR primer sequencing result, in table 5.
Table 5 criterion validation result
2.2 vertebrates derived component PCR method sensitivity tests
Plasmid is carried out gradient dilution, and the plasmid diluted carries out electrophoretic analysis after carrying out pcr amplification, finds that its minimal detectable concentration can reach 10
2copy number magnitude, is shown in Fig. 2.
Invertebrate species is various, all closely related with the daily life of the mankind in food, medicine, healthcare products, textile industry, handicraft industry, animal are viewed and admired etc., is enough to the daily daily life affecting the mankind.Meanwhile, vertebrates plays extremely important role at occurring in nature again, covers the extreme portions of biologic chain.In view of its representativeness, importance, ubiquity and popularity, the method when phase research invertebrate species is identified in office all has very significant meaning.
The present invention is according to multiple vertebrates Mitochondrial DNA (mtDNA) sequence announced in GenBank, devise a pair universal primer in its high conservative region and set up general PCR detection method, experimental result shows this primer highly versatile, achieve all effective amplifications of collecting sample, achieve the effective qualification to species in conjunction with sequencing result.
<110> Inspection & Quarantine Technology Center of Zhuhai Entry-Exit Inspection & Quarantine Bureau
The universal PC R of <120> vertebrates derived component detects primer and detection method
<130>
<160>2
<170>PatentInversion3.5
<210>1
<211>24
<212>DNA
<213> artificial sequence
<400>1
tgtttaccaaaaacatcacctcca24
<210>2
<211>21
<212>DNA
<213> artificial sequence
<400>2
agttaaagctccatagggtct21
Claims (5)
1. for detecting a general PCR primer pair for vertebrates derived component, it is characterized in that, described primer pair designs according to the consensus sequence of vertebrate Mitochondrial DNA.
2. the general PCR primer pair for detecting vertebrates derived component according to claim 1, it is characterized in that, the nucleotide sequence of described primer pair is as follows:
F-primer：5’-TGTTTACCAAAAACATCACCTCCA-3’；
R-primer：5’-AGTTAAAGCTCCATAGGGTCT-3’。
3. a PCR detection method for vertebrates derived component, comprises the steps:
(1) extract sample DNA as amplification template, vertebrates source property sample DNA is positive control;
(2) pcr amplification is carried out with the primer pair described in claim 1 or 2;
(3) gel electrophoresis is carried out to PCR product, whether contain musk deer derived component according in electrophoresis result judgement sample.
4. the PCR detection method of animal derived materials according to claim 3, it is characterized in that, consisting of of 25 μ LPCR reaction systems: 2 × TaqPCRMastermix12.5 μ L, 10 μm of each 1.0 μ L of ol/L upstream and downstream primer, DNA masterplate 2.0 μ L, moisturizing to 25 μ L.
5. the PCR detection method of animal derived materials according to claim 3, is characterized in that, PCR response procedures is: 94 DEG C of 4min; 94 DEG C of 30s, 58 DEG C of 30s, 72 DEG C of 30s, 35 circulations; 72 DEG C of 5min.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834461A (en) * | 2017-01-22 | 2017-06-13 | 上海出入境检验检疫局动植物与食品检验检疫技术中心 | The universal detection method of the generation of high flux two sequencing of animal derived materials in biological products |
| CN113046445A (en) * | 2021-03-30 | 2021-06-29 | 拱北海关技术中心 | DNA barcodes, primers, kit and method for identifying vertebrates |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102296111A (en) * | 2011-08-05 | 2011-12-28 | 中国肉类食品综合研究中心 | RT-PCR based method for determining component contents of specific meats in mixed meat products |
| CN104328195A (en) * | 2014-11-13 | 2015-02-04 | 西北民族大学 | Kit for detecting animal-derived components in feed and detection method of kit |
| CN104561271A (en) * | 2014-12-04 | 2015-04-29 | 珠海出入境检验检疫局检验检疫技术中心 | Visual DNA (deoxyribonucleic acid) chip kit and method for detecting multiple animal-derived components |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102296111A (en) * | 2011-08-05 | 2011-12-28 | 中国肉类食品综合研究中心 | RT-PCR based method for determining component contents of specific meats in mixed meat products |
| CN104328195A (en) * | 2014-11-13 | 2015-02-04 | 西北民族大学 | Kit for detecting animal-derived components in feed and detection method of kit |
| CN104561271A (en) * | 2014-12-04 | 2015-04-29 | 珠海出入境检验检疫局检验检疫技术中心 | Visual DNA (deoxyribonucleic acid) chip kit and method for detecting multiple animal-derived components |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834461A (en) * | 2017-01-22 | 2017-06-13 | 上海出入境检验检疫局动植物与食品检验检疫技术中心 | The universal detection method of the generation of high flux two sequencing of animal derived materials in biological products |
| CN106834461B (en) * | 2017-01-22 | 2021-01-15 | 上海出入境检验检疫局动植物与食品检验检疫技术中心 | Universal detection method for high-throughput next generation sequencing of animal-derived components in biological products |
| CN113046445A (en) * | 2021-03-30 | 2021-06-29 | 拱北海关技术中心 | DNA barcodes, primers, kit and method for identifying vertebrates |
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