CN105506028A - Method and system for manufacturing sugar by utilizing potato dregs - Google Patents
Method and system for manufacturing sugar by utilizing potato dregs Download PDFInfo
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- CN105506028A CN105506028A CN201511031729.6A CN201511031729A CN105506028A CN 105506028 A CN105506028 A CN 105506028A CN 201511031729 A CN201511031729 A CN 201511031729A CN 105506028 A CN105506028 A CN 105506028A
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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Abstract
The invention relates to a method and system for manufacturing sugar by utilizing potato dregs. The method comprises the following steps: firstly regulating the pH values of the potato dregs to be more than 4.8 and then adding amylase to carry out liquefaction; regulating the pH value of an obtained liquefaction liquid to be smaller than 4.8 and then adding pectinase and a saccharifying enzyme to carry out saccharification treatment to obtain a saccharification mash; centrifuging the saccharification mash and carrying out solid-liquid separation, thus obtaining a sugar solution. The sugar solution can be directly used in the field of yeast fermentation, serves as yeast culture bottom water or an original molasses diluent or serves as fed-batch sugar for yeast fermentation after being concentrated. The method and system achieve sugar manufacturing with the potato dregs at a dry substance concentration of 5-16% through the technology of manufacturing sugar by an enzymic method, are especially suitable for large-scale industrial production and have the effect of solving the problems that the potato dregs in the prior art can not be utilized in large scale, pollute the environment when dried in the sun outdoors and have low economic benefits.
Description
Technical field
The present invention relates to biological technical field, particularly relate to a kind of method and system utilizing potato slag to refine sugar.
Background technology
Can produce the by products such as a large amount of potato slags and enchylema when preparing starch (mainly sweet potato starch and yam starch), the wet potato slag moisture content ejected from production line centrifugal screen is greatly between 90%-95%.Wherein, in potato slag in most cells wall in conjunction with moisture approximately about 75%, free water content is greatly between 10%-20%.Due to the seasonality of Starch Production, will produce a large amount of potato slag in the short period of time, if do not contact with air from the potato slag of production line discharge, cleanliness factor is very high, can not to environment.If but potato slag carries out second dehydration not in time, will be difficult to long time stored, process just may cause very serious environmental pollution not in time.
The reason of potato Slag treatment difficulty is: 1, for large-scale Starch Production enterprise, and the potato quantity of slag of generation per hour is huge, if do not have good technological design, equipment cannot keep smooth and easy running, and the phenomenon of occluding device very easily occurs; 2, potato slag viscosity is large, and the potato slag of general dry substance concentration more than 5% just can not flow, and material shifts or mixes very difficult; 3, the separation of potato slag converted mash is the difficult problem of this area always, and the technological design do not had cannot reach desirable separating effect.
Potato Slag treatment way domestic is at present two kinds substantially: 1, naturally dry; 2, first mechanical dehydration is dried again.Naturally dry the risk that there is environmental pollution, it is high that mechanical dehydration dries the biological fodder quality made again, but need the equipment investment of wholesale, and production cost is very high.
External large-scale yam starch processing enterprise adopts belt filter press to carry out the dehydration of potato residue usually, then carries out solid state fermentation, potato slag is recycled fully.Rely on spontaneous fermentation cost that potato slag is produced into feed minimum, supplying products dairy cow farm, can accomplish that revenue and expenditure maintains an equal level and even also has certain income.But at home, be limited to the scale restriction of raiser around Starch Production producer, the comprehensive utilization of potato slag has some limitations.
Therefore, develop the post-treating method of a potato seed slag, reduce the risk even eliminating environmental pollution on the one hand, can turn waste into wealth generation economic benefit on the other hand, then have a very big significance.
Summary of the invention
First object of the present invention be for prior art exist defect and deficiency, provide a kind of method utilizing potato slag to refine sugar, described method comprises the steps:
(1) liquefy: regulate the pH value of potato slag to be greater than 4.8, and add amylase wherein and carry out liquefaction processing, obtain liquefier;
(2) saccharification: regulate the pH value of described liquefier to be less than 4.8, and add saccharifying enzyme wherein and polygalacturonase carries out saccharification process, obtain converted mash;
(3) be separated: centrifugal described converted mash, and carry out solid-liquid separation, obtain liquid glucose.
Potato slag of the present invention is preferably potato residues or sweet potato waste, more preferably the potato slag of dry matter content 5-16%, for the potato slag that dry matter content is less than 5%, because its dry matter content is low, does not possess the economy of operation; And dry matter content is greater than to the potato slag of 16%, the viscosity of its process later stage converted mash is too large, cannot further be separated, therefore method of the present invention is particularly suitable for the potato slag being separated dry matter content 5-16%, has good separating effect.
Wherein, in step (1), described amylase is the amylase that this area is commonly used, and as can be α-amylase or beta-amylase, the present invention is preferably α-amylase, and adopt α-amylase that liquefaction can be made more abundant, the glucose content obtained is higher.
Described diastatic addition is preferably 50-150U/g dry-matter, more preferably 70-100U/g dry-matter.
Before liquefying, need the pH value first regulating described potato slag, find after deliberation, when pH value is greater than 4.8, amylase can both well liquefy to potato slag, but on the one hand, due to potato slag poor fluidity when potato slag dry matter content is greater than 5%, the potato slag high to a large amount of viscosity regulates pH very difficult, on the other hand, if liquefier pH value is too high, then needs to add a large amount of acid make its pH value drop to less than 4.8 when carrying out saccharification, same operation is very difficult.Present inventor finds unexpectedly, when the pH regulating potato slag in step (1) is 4.8-5.1, just can guarantee that potato slag well liquefies; In addition, when in step (2), pH value is 4.2-4.8, liquefier also can be good at by saccharification, and importantly, because the pH value amplitude of accommodation is little, it is also more convenient to operate.
Described liquefaction processing is preferably carried out under 90-105 DEG C of condition, can liquefy completely at this condition starch, energy-conservation reduction sugar making cost.
Wherein, in step (2), potato slag viscosity is large, the viscosity how reducing potato slag is a key factor of potato slag sugaring, the application surprisingly finds the mode adopting saccharifying enzyme and the composite use of polygalacturonase, also reduces the viscosity of converted mash, solve a separation difficult problem for potato slag converted mash while saccharification, substantially increase production efficiency, achieve the extensive cheapness sugaring of potato slag.
Wherein, the polygalacturonase that described polygalacturonase can adopt this area conventional, such as can select in Rohapect MPE, polygalacturonase, pectin lyase one or more, the saccharifying enzyme that described saccharifying enzyme also can adopt this area conventional.The addition of preferred described polygalacturonase is the 1-5 ‰ of described liquefier dry matter weight, and the addition of described saccharifying enzyme is 80-400U/g dry-matter.
More preferably the addition of saccharifying enzyme is 150-250U/g dry-matter, and the addition of polygalacturonase is the 2.5-3.5% of described liquefier dry matter weight, on this basis, can obtain best saccharification result.
Described saccharification process is preferably carried out under 50-60 DEG C of condition.
The method that the present invention preferably utilizes potato slag to refine sugar comprises the steps:
(1) liquefy: with the potato slag of dry matter content 5-16% for raw material, regulate the pH value of described potato slag to 4.8-5.1, add amylase wherein according to 50-150U/g dry-matter, in 90-105 DEG C of liquefaction processing, obtain liquefier;
(2) saccharification: described liquefier is cooled to 50-60 DEG C, then the pH of described liquefier is regulated to be 4.2-4.8, add saccharifying enzyme according to 80-400U/g dry-matter, add polygalacturonase according to 1-5 ‰ ratio of dry matter weight in liquefier, under 50-60 DEG C of condition, saccharification process, obtains converted mash;
(3) be separated: centrifugal described converted mash, and carry out solid-liquid separation, to obtain final product.
Second object of the present invention is to provide the liquid glucose adopting any one method above-mentioned to prepare.
3rd object of the present invention is to provide a kind of system utilizing potato slag to refine sugar, and described system comprises the liquefying plant, saccharification apparatus and the tripping device that connect in turn.
Wherein, described tripping device comprises the horizontal screw centrifuge and disc separator that connect in turn.
Described liquefying plant is used for the liquefaction of potato slag, and comprise premixing tank and liquefied pot, the present invention preferably adopts the mode of steam ejection liquefaction to carry out premix to amylase and potato slag.
Described saccharification apparatus is used for carrying out saccharification to the potato slag after liquefaction processing, comprises saccharifying tank, and potato slag is transformed into converted mash under the acting in conjunction of saccharifying enzyme and polygalacturonase.
Described tripping device is used for being separated converted mash, and the mode that the present invention adopts horizontal screw centrifuge and disc separator to combinationally use, can be good at being separated converted mash, solves the very much not segregative problem of converted mash viscosity in prior art.
Be separated after for some time placed by the liquid glucose obtained through tripping device and easily occur muddiness, this is because the cell debris containing some lighter in weight in liquid glucose, cannot fully and completely be separated through disc separator.In order to obtain high-quality liquid glucose, preferred described tripping device also comprises the plate-and-frame filter press after being connected to described disc separator or diatomite filter, plate-and-frame filter press or diatomite filter is adopted further to be separated liquid glucose, thoroughly can separate the cell debris in liquid glucose, improve the quality of liquid glucose.
In order to the liquid glucose of obtained high density, preferred described system also comprises concentrating unit, and after described concentrating unit is positioned at described tripping device, described concentrating unit is preferably MVR vaporizer.
In a kind of concrete embodiment, system of the present invention comprises the premixing tank of 2 90 tons, the liquefied pot of 12 5.7 cubes, the saccharifying tank of 5 300 cubes, 4 treatment capacities are the horizontal screw centrifuge of 30 cubic meters/hour, the disc separator of two treatment capacities 50 cubic meters/hour, and plate-and-frame filter press.Adopt this kind of system potato slag that can process 50-60 ton dry matter content 5-16% per hour, the yield that converted mash is separated the clear liquid obtained is 60%, clear liquid sugar concentration is 5.5-6%, 600-700 ton liquid glucose (rolling over full sugar about 35-40 ton) can be obtained every day, significantly reduce the potato slag pollution on the environment that Starch Production enterprise produces, liquid glucose can be used in biological fermentation field, and economic benefit is improved significantly.
The method that any one utilizes potato slag to refine sugar of the present invention all can adopt any one potato slag above-mentioned system of refining sugar to realize, and when adopting this system to process potato slag, solid-liquid separation is abundant, and liquid glucose yield is high.
The present invention, by potato slag enzyme process sugar refining, achieves the sugaring of 5-16% dry substance concentration potato slag, is particularly suitable for large-scale industrial production, solves potato slag contaminate environment in prior art, the problem that economic benefit is low.Produce the liquid glucose that obtains and can be used for yeast fermentation, the yeast of production takes out body thing and biological fodder for the production of yeast, defines complete pollution-free industry chain circulation, and potato Slag treatment difficult problem when solving Starch Production also achieves splendid comprehensive utilization ratio.
Wherein, the application of liquid glucose in yeast fermentation has the following two kinds form:
1, using described liquid glucose directly as the diluent of water at the bottom of yeast culture and/or raw sugar honey.
2, described liquid glucose is concentrated, obtain the liquid glucose that sugared concentration is 28-30%, then for yeast-leavened stream sugaring.
Accompanying drawing explanation
Fig. 1 is the structural representation of potato slag sugaring system.
In figure: 1, premixing tank; 2, liquefied pot; 3, saccharifying tank; 4, horizontal screw centrifuge; 5, disc separator; 6, plate filter; 7, MVR thickner; 8, liquid glucose storage tank.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Embodiment 1
Utilize the method that potato slag is refined sugar, described method comprises the steps:
(1) liquefy: with the potato residues of dry matter content 5-9% for raw material, regulate the pH value 4.8-5.1 of described potato residues, add amylase wherein according to 80U/g dry-matter, in 90 DEG C of liquefaction processing, after iodine solution test liquefaction completely, obtain liquefier;
(2) saccharification: described liquefier is cooled to 50-55 DEG C, then the pH4.2-4.8 of described liquefier is regulated, add saccharifying enzyme according to 150U/g dry-matter, add polygalacturonase according to 1.5 ‰ ratios of liquefier dry matter weight, and saccharification process under 50-55 DEG C of condition, obtain converted mash;
(3) be separated: centrifugal described converted mash, and carry out solid-liquid separation, to obtain final product.
The sugared content of the liquid glucose adopting this kind of method to prepare is 3-6%.
Embodiment 2
Utilize the method that potato slag is refined sugar, described method comprises the steps:
(1) liquefy: with the sweet potato waste of dry matter content 8-12% for raw material, regulate the pH value 4.8-5.1 of described sweet potato waste, add amylase wherein according to 80U/g dry-matter, in 100 DEG C of liquefaction processing, after iodine solution test liquefaction completely, obtain liquefier;
(2) saccharification: described liquefier is cooled to 50-55 DEG C, then the pH4.2-4.8 of described liquefier is regulated, add saccharifying enzyme according to 200U/g dry-matter, add polygalacturonase according to 3.5 ‰ ratios of liquefier dry-matter, and saccharification process under 50-55 DEG C of condition, obtain converted mash;
(3) be separated: centrifugal described converted mash, and carry out solid-liquid separation, to obtain final product.
The sugared content of the liquid glucose adopting this kind of method to prepare is 5-9%.
Embodiment 3
Utilize the method that potato slag is refined sugar, described method comprises the steps:
(1) liquefy: with the sweet potato waste of dry matter content 12-16% for raw material, regulate the pH value 4.8-5.1 of described sweet potato waste, add amylase wherein according to 100U/g dry-matter, in 100 DEG C of liquefaction processing, after iodine solution test liquefaction completely, obtain liquefier;
(2) saccharification: described liquefier is cooled to 50-55 DEG C, then the pH4.2-4.8 of described liquefier is regulated, add saccharifying enzyme according to 250U/g dry-matter, add polygalacturonase according to 5 ‰ ratios of liquefier dry-matter, and saccharification process under 50-55 DEG C of condition, obtain converted mash;
(3) be separated: centrifugal described converted mash, and carry out solid-liquid separation, to obtain final product.
The sugared content of the liquid glucose adopting this kind of method to prepare is 10-13%.
Embodiment 4
The present embodiment provides a kind of most preferred potato slag sugaring system, comprises the liquefying plant, saccharification apparatus, tripping device, concentrating unit and the storing unit that connect in turn;
Wherein, described liquefying plant comprises 2 90 tons of premixing tanks 1, and the liquefied pot 2 (only illustrating in figure) of 12 5.7 cubes.
Described saccharification apparatus comprises the saccharifying tank 3 (only illustrating 1 in figure) of 5 300 cubes.
Described tripping device comprises 4 treatment capacities to be horizontal screw centrifuge 4,2 treatment capacities of 30 cubic meters/hour be disc separator 5,1 plate-and-frame filter press 6 of 50 cubic meters/hour.
Described concentrating unit comprises the MVR vaporizer 7 of 1 treatment capacity 20-25 cubic meter/hour.
Described storing unit is liquid glucose storage tank 8.
Embodiment 5
The present embodiment provides the system utilizing embodiment 4 to carry out the method for potato slag sugaring, comprises the steps:
(1) pH value regulating 8-12% dry substance concentration potato slag is 4.8-5.1, by the mode of steam ejection liquefaction, amylase and potato slag are mixed at premixing tank 1 according to the amount of 80U/g dry-matter, then in liquefied pot 2, liquefy in 100 DEG C, after iodine examination is qualified, obtain liquefier;
(2) described liquefier is cooled to 50-55 DEG C, then its pH value is regulated to be 4.2-4.8, add saccharifying enzyme according to 200U/g dry-matter, add polygalacturonase according to 3.5 ‰ ratios of liquefier dry matter weight, under 50-60 DEG C of condition, in saccharifying tank 3, carry out saccharification process, obtain converted mash;
(3) use horizontal screw centrifuge 4 to carry out centrifugal treating to described converted mash, when described converted mash is limpider, adopt the mode of 4 horizontal screw centrifuge parallel connections to carry out centrifugal; When described converted mash separating effect is bad time, adopt horizontal screw centrifuge 2 series connection 2 parallel connections, the mode of centrifugal twice processes; Be separated the material obtained to be separated through disc separator 5, supernatant liquor is liquid glucose;
(4), when described liquid glucose leaves standstill for some time appearance muddiness, the step adopting plate-and-frame filter press 6 to carry out solid-liquid separation can be increased.
(5) according to the purposes of liquid glucose, the step using MVR vaporizer 7 to concentrate to described liquid glucose can be increased.
(6) liquid glucose after process stores via liquid glucose storage tank 8.
This kind of method potato slag that can process 50-60 ton dry matter content 5-16% per hour, the yield that converted mash is separated the clear liquid obtained is 60%, and clear liquid sugar concentration is 5.5-6%, can obtain 600-700 ton liquid glucose (rolling over full sugar about 35-40 ton) every day.
Embodiment 1 or 2 or 3 or 5 liquid glucose prepared can be applicable to yeast fermentation, and its concrete application method has two kinds, and one is using liquid glucose directly as water at the bottom of yeast culture, or as raw sugar honey diluent; Another kind first the liquid glucose of lower concentration is concentrated into 28-30%, then as yeast-leavened stream sugaring.
Comparative example 1
The method that this comparative example utilizes potato slag to refine sugar is identical with embodiment 2, and its difference is only only to add saccharifying enzyme in step (2), found that converted mash viscosity is large, cannot be separated.
Although above with general explanation, embodiment and test, the present invention is described in detail, and on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (9)
1. the method utilizing potato slag to refine sugar, is characterized in that, described method comprises the steps:
(1) liquefy: regulate the pH value of potato slag to be greater than 4.8, and add amylase wherein and carry out liquefaction processing, obtain liquefier;
(2) saccharification: regulate the pH of described liquefier to be less than 4.8, and add saccharifying enzyme wherein and polygalacturonase carries out saccharification process, obtain converted mash;
(3) be separated: centrifugal described converted mash, and carry out solid-liquid separation, obtain liquid glucose.
2. method according to claim 1, is characterized in that: described potato slag is preferably potato residues or sweet potato waste, more preferably the potato slag of dry matter content 5-16%.
3. method according to claim 1 and 2, is characterized in that: described diastatic addition is 50-150U/g dry-matter, more preferably 70-100U/g dry-matter;
And/or the addition of described polygalacturonase is the 1-5 ‰ of dry matter weight in described liquefier, more preferably 2.5-3.5 ‰;
And/or the addition of described saccharifying enzyme is 80-400U/g dry-matter, more preferably 150-250U/g dry-matter.
4., according to the arbitrary described method of claim 1-3, it is characterized in that: described liquefaction processing is carried out under 90-105 DEG C of condition;
And/or described saccharification process is carried out under 50-60 DEG C of condition.
5., according to the arbitrary described method of claim 1-4, it is characterized in that: in step (1), the pH value regulating potato slag is 4.8-5.1;
And/or in step (2), the pH value regulating potato slag is 4.2-4.8.
6. method according to claim 1, is characterized in that, described method comprises the steps:
(1) liquefy: with the potato slag of dry matter content 5-16% for raw material, regulate the pH value of described potato slag to be greater than 4.8, add amylase wherein according to 50-150U/g dry-matter, in 90-105 DEG C of liquefaction processing, obtain liquefier;
(2) saccharification: described liquefier is cooled to 50-60 DEG C, then the pH of described liquefier is regulated to be less than 4.8, add saccharifying enzyme according to 80-400U/g dry-matter, add polygalacturonase according to dry matter weight 1-5 ‰ ratio in liquefier, under 50-60 DEG C of condition, saccharification process, obtains converted mash;
(3) be separated: centrifugal described converted mash, and carry out solid-liquid separation, to obtain final product.
7. the liquid glucose for preparing of the arbitrary described method of claim 1-6.
8. the system utilizing potato slag to refine sugar, is characterized in that: described system comprises the liquefying plant, saccharification apparatus and the tripping device that connect in turn, the horizontal screw centrifuge that described tripping device preferably connects in turn and disc separator; Further preferred described tripping device also comprises the plate-and-frame filter press after being connected to described disc separator or diatomite filter.
9. system according to claim 7, is characterized in that: described system also comprises the concentrating unit after being connected to described tripping device, and described concentrating unit is preferably MVR vaporizer.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201511031729.6A CN105506028A (en) | 2015-12-31 | 2015-12-31 | Method and system for manufacturing sugar by utilizing potato dregs |
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| CN201511031729.6A CN105506028A (en) | 2015-12-31 | 2015-12-31 | Method and system for manufacturing sugar by utilizing potato dregs |
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