CN105497070A - Preparation method and application of sheep embryo living cell freeze-drying preparation - Google Patents
Preparation method and application of sheep embryo living cell freeze-drying preparation Download PDFInfo
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- CN105497070A CN105497070A CN201510995069.7A CN201510995069A CN105497070A CN 105497070 A CN105497070 A CN 105497070A CN 201510995069 A CN201510995069 A CN 201510995069A CN 105497070 A CN105497070 A CN 105497070A
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- 241001494479 Pecora Species 0.000 title claims abstract description 38
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 32
- 238000004108 freeze drying Methods 0.000 title claims abstract description 30
- 239000006285 cell suspension Substances 0.000 claims abstract description 11
- 239000000284 extract Substances 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 235000019687 Lamb Nutrition 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 102000004142 Trypsin Human genes 0.000 claims description 4
- 108090000631 Trypsin Proteins 0.000 claims description 4
- 210000004185 liver Anatomy 0.000 claims description 4
- 210000000056 organ Anatomy 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 239000012588 trypsin Substances 0.000 claims description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 3
- 102000029816 Collagenase Human genes 0.000 claims description 3
- 108060005980 Collagenase Proteins 0.000 claims description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 3
- 229960002424 collagenase Drugs 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000009413 insulation Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 230000003252 repetitive effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 210000001541 thymus gland Anatomy 0.000 claims description 3
- 102000008186 Collagen Human genes 0.000 claims description 2
- 108010035532 Collagen Proteins 0.000 claims description 2
- 239000002775 capsule Substances 0.000 claims description 2
- 210000001198 duodenum Anatomy 0.000 claims description 2
- 230000002255 enzymatic effect Effects 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims description 2
- 210000002216 heart Anatomy 0.000 claims description 2
- 210000003734 kidney Anatomy 0.000 claims description 2
- 239000008176 lyophilized powder Substances 0.000 claims description 2
- 210000000496 pancreas Anatomy 0.000 claims description 2
- 239000008188 pellet Substances 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 210000000952 spleen Anatomy 0.000 claims description 2
- 230000008520 organization Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 7
- 238000000034 method Methods 0.000 abstract description 7
- 239000000047 product Substances 0.000 abstract description 6
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 238000000746 purification Methods 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract 2
- 239000004480 active ingredient Substances 0.000 abstract 1
- 235000015872 dietary supplement Nutrition 0.000 abstract 1
- 210000002257 embryonic structure Anatomy 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 5
- 210000002826 placenta Anatomy 0.000 description 4
- 230000003169 placental effect Effects 0.000 description 4
- 238000002372 labelling Methods 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000001605 fetal effect Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 206010000234 Abortion spontaneous Diseases 0.000 description 1
- 101100117236 Drosophila melanogaster speck gene Proteins 0.000 description 1
- 208000006399 Premature Obstetric Labor Diseases 0.000 description 1
- 206010036600 Premature labour Diseases 0.000 description 1
- 206010040925 Skin striae Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000002605 anti-dotal effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000020411 cell activation Effects 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000012173 estrus Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 208000015994 miscarriage Diseases 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000002990 parathyroid gland Anatomy 0.000 description 1
- 208000026440 premature labor Diseases 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000037394 skin elasticity Effects 0.000 description 1
- 208000000995 spontaneous abortion Diseases 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Epidemiology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Reproductive Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Virology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Gynecology & Obstetrics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pregnancy & Childbirth (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to the field of dietary supplements and biological pharmacy, and particularly discloses a preparation method and application of a sheep embryo living cell freeze-drying preparation. Fresh sheep embryos are selected, a purified living cell freeze-drying preparation of all positions is obtained through the method of cell suspension preparation, cell separation and purification and cell freeze drying, and all the nutritional ingredients and active ingredients of vitaswiss can be kept to the maximum extent through the living cell freeze-drying preparation. The steps are simple, operation is convenient, design is reasonable, the obtained product is high in quality, the nutritional ingredients and activity of vitaswiss can be kept to the maximum extent, the effects are high, and the preparation method and application are suitable for wide application and popularization.
Description
(1) technical field
The present invention relates to nutrient and healthcare products and field of biological pharmacy, particularly a kind of preparation method of sheep embryo viable cell freeze-drying preparation and application thereof.
(2) background technology
Sheep placental extract comprises sheep placenta and sheep embryo extract, and sheep embryo extract and sheep placenta are two concepts being easy to obscure, and both have the difference of essence.Placenta extract is the garbage in statement transmittance process, and eggembryosin is then the factor of the most aobvious vigor of life growth.The active substance extracted from embryo is more than extraction of substance difficulty from Placenta Hominis, also precious more expensive, and the composition of active substance also differs widely.
Sheep embryo extract can regulate human endocrine, improves human body skin situation comprehensively and effectively, has looks improving and the skin nourishing, skin whitening, strengthens skin elasticity, black speck of dispelling, prevents wrinkle, striae gravidarum and slow down aging, can prevent infertility, miscarriage, premature labor; Adjustment hormone, increases blood manufacture, improves anemia, the deficiency of vital energy, strengthens cell viability, accelerates postpartum recovery, helps cesarean lactation; Improve human immunologic function, postpone Women’s climacteric, alleviate numerous effects such as climacteric syndrome disease.
1931, Switzerland Borrow Ni Han doctor (Dr.PaulNiehans) first by a kind of fresh and alive cell infusion that extracts from lamb embryo to parathyroid gland impaired be dying in patient body, and successfully saved its life, this patient passed away in 1962 afterwards, die at the age of 89 years old, the initial origin of sheep embryo extract cell activation therapy that Here it is, its discovery has landmark meaning to the mankind at the medical development in defying age field.And to today, the preparation technique of Switzerland's sheep embryo extract is still the state secret of Switzerland, Switzerland is the country uniquely grasping sheep placental extract Refining technology in the world, uniquely holds formal sheep placental extract injection license, can carry out the country of sheep placental extract antidotal therapy.The competent cell of injection is through blood absorption, and the immune system finally acting on human body is to the effect of producing.
(3) summary of the invention
The present invention, in order to make up the deficiencies in the prior art, provides preparation method and the application thereof of the sheep embryo viable cell freeze-drying preparation that a kind of step is simple, product purity is high.
The present invention is achieved through the following technical solutions:
A preparation method for sheep embryo viable cell freeze-drying preparation, with the lamb embryo at 3 monthly ages for handling object, comprises the steps:
(1) lamb embryo is cleaned with sterilized water, then adopt mass fraction be 75% ethanol carry out surface sterilization, in aseptic operating platform, cut liver, pancreas, kidney, heart, duodenum, each organ of Thymus and spleen, shredded with operating scissors, collect respectively;
(2) each organ-tissue enzymolysis of being taken up in order of priority employing trypsin and collagen protein enzymatic solution to shred, to destroy intercellular protein connection, filters and obtains corresponding cell suspension;
(3) various cell suspension is placed in centrifuge tube respectively centrifugal, obtains various types of cells pellet, repetitive operation 2 times;
(4) slowly beat cell precipitation agglomerate with the trehalose isosmotic solution that mass fraction is 10%, make cell suspension, insulation 10min;
(5) various types of cells suspension is added in container respectively, IQF in-70 DEG C of refrigerators;
(6) container is moved into freeze-drying apparatus, lyophilizing lyophilization cycle, makes cell dehydration;
(7) container containing freeze drying cell is stored at 2-8 DEG C, obtain the product that can recover former saturation state in distilled water.
The present invention for raw material with fresh sheep embryo, extracts embryo's living cells through biotechnology, through lyophilizing, finally makes and have bioactive sheep embryo viable cell freeze-drying preparation; The various nutritional labeling that the embryo's living cells obtained can farthest keep sheep embryo extract to possess, with active, makes it play maximum effect.
Its preferred technical characteristic is:
In step (2), each organ-tissue first uses the trypsin solution enzymolysis 10min of 0.05g/L, then uses the Collagenase enzymolysis 10min of 1g/L.
In step (3), Centrifugal Environment is 4 DEG C, and centrifugal force is 50g, and the time is 15min.
In step (6), a lyophilization cycle is 15 days, and the water content after cell lyophilizing is 30%.
The application of sheep embryo viable cell freeze-drying preparation of the present invention, for the preparation of sheep embryo extract capsule, sheep embryo extract oral formulations or sheep embryo extract lyophilized powder, product adopts oral method, sheep embryo extract is made to act on human body with safest form, the fissional speed of effective enhancing, improve the regeneration function of aged cells, make the process of human senility be effectively controlled and alleviate, body is again full of vitality.
The present invention selects fresh sheep embryo, through preparing the method for cell suspension, separating-purifying cell, cell lyophilizing, obtain each position viable cell freeze-drying preparation of purification, this viable cell freeze-drying preparation can keep whole nutritional labeling and the active component of sheep embryo extract to greatest extent.
Step of the present invention is simple, and easy to operate, reasonable in design, the product quality obtained is high, can farthest keep its nutritional labeling and activity, and effect is comparatively large, is suitable for wide popularization and application.
(4) detailed description of the invention
Embodiment:
(1) select healthy and strong, the estrus cycle, normal ewe was as replace-conceive sheep, and all sheep are through laggard row artificial fertilizationes of process such as quarantine, epidemic prevention, dippings;
(2) treat that fetal lamb grows to about 3 months, modus operandi takes out fetal lamb, and use sterile water wash clean, the ethanol of 75% carries out surface sterilization;
(3) under aseptic condition, cut each organs such as the thymus of embryo, liver, normal saline cleans, 75% alcohol-pickled 5min sterilizing, finally clean with normal saline flushing, with aseptic operation shears in aseptic operating platform, each organ is shredded and is placed in plate, collect respectively;
(4) first use the trypsinization 10min of 0.05g/L, then use the collagenase digesting 10min of 1g/L;
(5) various types of cells suspension is placed in respectively Hunan permanent promise 5-5R low speed refrigerated centrifuge, 4 DEG C, centrifugal 15 minutes of 100rpm, repetitive operation 2 times, obtains cell precipitation agglomerate;
(6) supernatant discarded, slowly blows and beats cell with the trehalose isosmotic solution of 10%, makes cell suspension, insulation 10min;
(7) cell suspension is added in container respectively, IQF 30min in-70 DEG C of refrigerators;
(8) container is moved into freeze-drying apparatus, lyophilizing lyophilization cycle, making water content be 30%, lyophilization cycle is 15 days;
(9) by the containers store containing freeze drying cell under the cryogenic temperature of 2-8 DEG C, in order to recovering former saturation state afterwards in distilled water.
Claims (5)
1. the preparation method of a sheep embryo viable cell freeze-drying preparation, with the lamb embryo at 3 monthly ages for handling object, it is characterized by, comprise the steps: that lamb embryo cleans with sterilized water by (1), then adopt mass fraction be 75% ethanol carry out surface sterilization, in aseptic operating platform, cut liver, pancreas, kidney, heart, duodenum, each organ of Thymus and spleen, shredded with operating scissors, collect respectively; (2) each organ-tissue enzymolysis of being taken up in order of priority employing trypsin and collagen protein enzymatic solution to shred, to destroy intercellular protein connection, filters and obtains different types of cell suspension; (3) various cell suspension is placed in centrifuge tube respectively centrifugal, obtains various types of cells pellet, repetitive operation 2 times; (4) slowly beat cell precipitation agglomerate with the trehalose isosmotic solution that mass fraction is 10%, make cell suspension, insulation 10min; (5) cell suspension is added in container respectively, IQF in-70 DEG C of refrigerators; (6) container is moved into freeze-drying apparatus, lyophilizing lyophilization cycle; (7) container containing freeze drying cell is stored at 2-8 DEG C, obtain the product that can recover former saturation state in distilled water.
2. the preparation method of sheep embryo viable cell freeze-drying preparation according to claim 1, is characterized in that: in step (2), and liver organization first uses the trypsin solution enzymolysis 10min of 0.05g/L, then uses the Collagenase enzymolysis 10min of 1g/L.
3. the preparation method of sheep embryo viable cell freeze-drying preparation according to claim 1, it is characterized in that: in step (3), Centrifugal Environment is 4 DEG C, and centrifugal force is 50g, and the time is 15min.
4. the preparation method of sheep embryo viable cell freeze-drying preparation according to claim 1, is characterized in that: in step (6), and a lyophilization cycle is 15 days, and the water content after cell lyophilizing is 30%.
5. the application of sheep embryo viable cell freeze-drying preparation according to claim 1, is characterized in that: for the preparation of sheep embryo extract capsule, sheep embryo extract oral formulations or sheep embryo extract lyophilized powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510995069.7A CN105497070A (en) | 2015-12-28 | 2015-12-28 | Preparation method and application of sheep embryo living cell freeze-drying preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510995069.7A CN105497070A (en) | 2015-12-28 | 2015-12-28 | Preparation method and application of sheep embryo living cell freeze-drying preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105497070A true CN105497070A (en) | 2016-04-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510995069.7A Pending CN105497070A (en) | 2015-12-28 | 2015-12-28 | Preparation method and application of sheep embryo living cell freeze-drying preparation |
Country Status (1)
| Country | Link |
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| CN (1) | CN105497070A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106562995A (en) * | 2016-10-26 | 2017-04-19 | 沈会强 | Preparation method for preparing swan embryos and application of swan embryos |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1227745A (en) * | 1998-03-03 | 1999-09-08 | 徐宁忠 | Process for producing freeze-dried powder of sheep embryo by vacuum freezing |
| CN1242196A (en) * | 1998-07-17 | 2000-01-26 | 西安阳光保健品开发有限责任公司 | Method for preparing goat fetal hormone injection |
| CN1843377A (en) * | 2006-02-13 | 2006-10-11 | 北京凯诗伦生物技术有限公司 | Sheep placenta essence and its preparing process |
| CN102327290A (en) * | 2011-07-12 | 2012-01-25 | 湖州康海斯生物科技有限公司 | Refined sheep placenta extract prepared from sheep embryo and sheep placental peptide and preparation method |
| CN102688176A (en) * | 2012-06-14 | 2012-09-26 | 扬州扬大联环药业基因工程有限公司 | Sheet embryo extract freeze-dried powder and preparation technology |
-
2015
- 2015-12-28 CN CN201510995069.7A patent/CN105497070A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1227745A (en) * | 1998-03-03 | 1999-09-08 | 徐宁忠 | Process for producing freeze-dried powder of sheep embryo by vacuum freezing |
| CN1242196A (en) * | 1998-07-17 | 2000-01-26 | 西安阳光保健品开发有限责任公司 | Method for preparing goat fetal hormone injection |
| CN1843377A (en) * | 2006-02-13 | 2006-10-11 | 北京凯诗伦生物技术有限公司 | Sheep placenta essence and its preparing process |
| CN102327290A (en) * | 2011-07-12 | 2012-01-25 | 湖州康海斯生物科技有限公司 | Refined sheep placenta extract prepared from sheep embryo and sheep placental peptide and preparation method |
| CN102688176A (en) * | 2012-06-14 | 2012-09-26 | 扬州扬大联环药业基因工程有限公司 | Sheet embryo extract freeze-dried powder and preparation technology |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106562995A (en) * | 2016-10-26 | 2017-04-19 | 沈会强 | Preparation method for preparing swan embryos and application of swan embryos |
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Application publication date: 20160420 |