CN105483044A - Anabaena culture medium - Google Patents
Anabaena culture medium Download PDFInfo
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- CN105483044A CN105483044A CN201511021395.4A CN201511021395A CN105483044A CN 105483044 A CN105483044 A CN 105483044A CN 201511021395 A CN201511021395 A CN 201511021395A CN 105483044 A CN105483044 A CN 105483044A
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- anabena
- substratum
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention provides an anabaena culture medium which is prepared from a Bg11 culture medium, NaNO3 and K2HPO4, wherein by total volume of the Bg11 culture medium, the concentration of NaNO3 is 3 g/L-10 g/L, and the concentration of K2HPO4 is 0.08 g/L-0.5 g/L. The anabaena culture medium can promote mass growth of anabaena in a short time, is rich in organic matter, overcomes the defect that the grease content is reduced during mass growth of anabaena, and provides a research method and concept for researching that eutrophication matter brought by volcanic eruption and upwelling can promote growth of Neo-Mesoproterozoic maternal hydrocarbon-generating material-crynobacteria and organic matter enrichment.
Description
Technical field
The present invention relates to a kind of substratum, particularly a kind of anabena substratum, belong to ancient oil-gas exploration experimental technique field.
Background technology
Anabena, as Azotica, be it is found that there is fertilizer efficiency effect to paddy rice very early, and the application of south China Rice Cropping obtains good effect of increasing production and objective economic benefit.
From the angle of oil-gas geology Organic Matter Enrichment, blue-green algae is as the main source material of Neoproterozoic organic matter hydrocarbon generation in China, and its raw hydrocarbon potentiality and growth scale need to be studied further.The grease of anabena as renewable source, can be equivalent to oil, can be used as one of energy of exploitation future.
Under normal circumstances, the quick growth of anabena can cause organic accumulation to decline usually, increases fat content, need suitable substratum and cultivate while how increasing anabena output fast.
invention/content
In order to solve the problems of the technologies described above, the object of the present invention is to provide a kind of anabena substratum, this anabena substratum can make anabena while short-term grows fast, promote organic a large amount of enrichment.
In order to realize above-mentioned technical purpose, the invention provides a kind of anabena substratum, this anabena substratum comprises Bg11 substratum, NaNO
3, and K
2hPO
4, wherein, with the entire volume of Bg11 substratum, NaNO
3concentration be 3g/L-10g/L, K
2hPO
4concentration be 0.08g/L-0.5g/L.
In anabena substratum provided by the invention, preferably, with the entire volume of Bg11 substratum, NaNO
3concentration be 5g/L-7g/L.
In anabena substratum provided by the invention, preferably, with the entire volume of Bg11 substratum, K
2hPO
4concentration be 0.1g/L-0.4g/L.
In anabena substratum provided by the invention, preferably, the intensity of illumination of this anabena substratum when cultivating anabena is 20 μm of ol/m
2/ s-30 μm of ol/m
2/ s.
In anabena substratum provided by the invention, preferably, the envrionment temperature of this anabena substratum when cultivating anabena is 25 DEG C-27 DEG C.
In anabena substratum provided by the invention, preferably, this anabena substratum keeps passing into of air in cultivation anabena process.
In anabena substratum provided by the invention, preferably, the speed that passes into of this anabena substratum air in cultivation anabena process is 150mL/min-250mL/min.
In anabena substratum provided by the invention, preferably, the cultivation period of this anabena substratum is 10 days-15 days; More preferably, the cultivation period of this anabena substratum is 12 days.
Anabena substratum provided by the invention can impel that anabena is organic while raised growth at short notice, and enrichment occurs, solving the problem of anabena fat content deficiency while raised growth, for studying rich phosphorus substance in research oil-gas geology, a kind of research method being provided to microfossil Organic Matter Enrichment simultaneously.
Accompanying drawing explanation
Fig. 1 is the structural representation of anabena culture apparatus in embodiment.
Fig. 2 is the growing state comparison diagram of anabena in reference examples 1 and embodiment 1-embodiment 3.
Fig. 3 is the fat content comparison diagram after reference examples 1 is tested with embodiment 1-embodiment 3.
Main Reference nomenclature
1 inlet mouth 2 air outlet 3 sealing plug 4 air pump
Embodiment
In order to there be understanding clearly to technical characteristic of the present invention, object and beneficial effect, existing following detailed description is carried out to technical scheme of the present invention, but can not be interpreted as to of the present invention can the restriction of practical range.
Cultivate being all used in artificial climate incubator in reference examples 1 and embodiment 1-3, the structure of concrete device as shown in Figure 1, this anabena culture apparatus comprises inlet mouth 1, air outlet 2, sealing plug 3, air pump 4 and Erlenmeyer flask, and the variation range of intensity of illumination is 20 μm of ol/m
2/ s-30 μm of ol/m
2/ s, temperature 25 DEG C-27 DEG C, keeps passing into of air in cultivating process, and the cycle of cultivation is 12 days.
Laboratory sample:
Growth indexes measures: every 1 day after inoculation, get respectively in different parallel group of algae Ye3mLYu island UV-2000 spectrophotometer and detect OD680.
Method for testing and analyzing
Grease extracts and assay: prepare a 5mL size, with the vial of tetrafluoroethylene lid, accurately take lyophilize algae powder M1mg, the algae powder claimed is transferred in above-mentioned vial, and add magnetic stir bar, add the dimethyl sulfoxide (DMSO) methanol solution (V/V) of 10%, extracting 30 minutes is stirred at 50 DEG C of water bath condition lower magnetic forces, with after through 4000rpm centrifugal 10 minutes, supernatant liquor is transferred in another 25mL vial, extract twice in this way, ether is added: normal hexane mixed solution (1:1V/V) in residue algae-residue, magnetic agitation drawer 30 minutes, 4000rpm is centrifugal afterwards, supernatant liquor is transferred in above-mentioned 25mL vial, extract 3 times in this way, pure water is added in the supernatant liquor of 5 extractions, make normal hexane, ether, methyl alcohol, water four kinds of ratios are 1:1:1:1, through the centrifugal 5min of 1500rpm after abundant concussion mixing, pipette upper organic phase, lower floor's inorganic phase adds ether again: normal hexane mixed solution (1:1), centrifugal extraction twice, prepare the vial of a 1.5mL capacity and the M2 that weighs, after upper organic phase merges after nitrogen is concentrated, transfer concentrated solution is to above-mentioned vial, nitrogen is concentrated into lyophilize again after constant weight, weigh, total lipid content LC (%DW) is calculated as follows: %Totallipids=100 × [M3-M2]/M1.
Reference examples 1
Present embodiments provide a kind of anabena substratum, be that the anabena kind being 0.03 by the OD value of 15mL is inoculated in the substratum of the Bg11 of the 85mL of standard, arrange three parallel group.
Embodiment 1
Present embodiments provide a kind of anabena substratum, be that the anabena kind being 0.03 by the OD value of 15mL is inoculated in the substratum of the Bg11 of the 85mL of standard, the anabena kind with this OD value arranges three parallel group;
Add NaNO
3(concentration is 5g/L), K
2hPO
4(concentration is 0.16g/L).
Embodiment 2
Present embodiments provide a kind of anabena substratum, be that the anabena kind being 0.03 by the OD value of 15mL is inoculated in the substratum of the Bg11 of the 85mL of standard, the anabena kind with this OD value arranges three parallel group;
Add NaNO
3(concentration is 6g/L), K
2hPO
4(concentration is 0.24g/L).
Embodiment 3
Present embodiments provide a kind of anabena substratum, be that the anabena kind being 0.03 by the OD value of 15mL is inoculated in the substratum of the Bg11 of the 85mL of standard, the anabena kind with this OD value arranges three parallel group;
Add NaNO
3(concentration is 7g/L), K
2hPO
4(concentration is 0.32g/L).
Experimental result
See Fig. 2, with reference to comparative example 1, the increment showed increased of embodiment 1-embodiment 3, and increment is embodiment 3 > embodiment 2 > embodiment 1 > reference example 1, the concentration of nitrogen and phosphorus of this scope is described, concentration of nitrogen and phosphorus is higher, is more conducive to growth and the increment of Microcystis aeruginosa.
See Fig. 3, with reference to comparative example 1, the fat content of embodiment 1-embodiment 3 is that multiple increases, and grease increasing amount is embodiment 3 > embodiment 2 > embodiment 1 > comparative example 1, the concentration of nitrogen and phosphorus of this scope is described, concentration of nitrogen and phosphorus is higher, is more conducive to the accumulation of Microcystis aeruginosa grease.
Above embodiment illustrates, anabena substratum of the present invention can make anabena while short-term grows fast, promote organic a large amount of enrichment.
Claims (9)
1. an anabena substratum, is characterized in that, this anabena substratum comprises Bg11 substratum, NaNO
3, and K
2hPO
4, wherein, with the entire volume of Bg11 substratum, NaNO
3concentration be 3g/L-10g/L, K
2hPO
4concentration be 0.08g/L-0.5g/L.
2. anabena substratum according to claim 1, is characterized in that, with the entire volume of Bg11 substratum, and described NaNO
3concentration be 5g/L-7g/L.
3. anabena substratum according to claim 1, is characterized in that, with the entire volume of Bg11 substratum, and described K
2hPO
4concentration be 0.1g/L-0.4g/L.
4. anabena substratum according to claim 1, is characterized in that, the intensity of illumination of this anabena substratum when cultivating anabena is 20 μm of ol/m
2/ s-30 μm of ol/m
2/ s.
5. anabena substratum according to claim 1, is characterized in that, the envrionment temperature of this anabena substratum when cultivating anabena is 25 DEG C-27 DEG C.
6. anabena substratum according to claim 1, is characterized in that, this anabena substratum keeps passing into of air in cultivation anabena process.
7. the anabena substratum according to claim 1 or 6, is characterized in that, the speed that passes into of this anabena substratum air in cultivation anabena process is 150mL/min-250mL/min.
8. anabena substratum according to claim 1, is characterized in that, the cultivation period of this anabena substratum is 10 days-15 days.
9. anabena substratum according to claim 8, is characterized in that, the cultivation period of this anabena substratum is 12 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511021395.4A CN105483044A (en) | 2015-12-31 | 2015-12-31 | Anabaena culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511021395.4A CN105483044A (en) | 2015-12-31 | 2015-12-31 | Anabaena culture medium |
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| Publication Number | Publication Date |
|---|---|
| CN105483044A true CN105483044A (en) | 2016-04-13 |
Family
ID=55670324
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201511021395.4A Pending CN105483044A (en) | 2015-12-31 | 2015-12-31 | Anabaena culture medium |
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|---|---|
| CN (1) | CN105483044A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106479949A (en) * | 2016-09-08 | 2017-03-08 | 上海交通大学 | Activity In Anabaena Azotica cell is by the restorative procedure of ALS inhibitor class herbicide injury |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102220241A (en) * | 2011-05-06 | 2011-10-19 | 中国科学院青岛生物能源与过程研究所 | Cultivated fresh water microalgae and application thereof in production of biodiesel and nervonic acid |
| CN102229889A (en) * | 2011-05-31 | 2011-11-02 | 中国科学院青岛生物能源与过程研究所 | Chlorella, its culture method and applications |
| CN104372044A (en) * | 2014-11-14 | 2015-02-25 | 天津大学 | Method for preparing biofuel, biogas and fertilizer for vehicles by all-component utilization of microalgae |
-
2015
- 2015-12-31 CN CN201511021395.4A patent/CN105483044A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102220241A (en) * | 2011-05-06 | 2011-10-19 | 中国科学院青岛生物能源与过程研究所 | Cultivated fresh water microalgae and application thereof in production of biodiesel and nervonic acid |
| CN102229889A (en) * | 2011-05-31 | 2011-11-02 | 中国科学院青岛生物能源与过程研究所 | Chlorella, its culture method and applications |
| CN104372044A (en) * | 2014-11-14 | 2015-02-25 | 天津大学 | Method for preparing biofuel, biogas and fertilizer for vehicles by all-component utilization of microalgae |
Non-Patent Citations (5)
| Title |
|---|
| 孟顺龙 等: "氮磷质量浓度对普通小球藻和鱼腥藻生长竞争的影响", 《生态环境学报》 * |
| 季祥 等: "富油能源微藻斜生栅藻异养培养条件的优化", 《渔业现代化》 * |
| 杨凯 等: "不同质量浓度NaNO3对3种微藻生长及总脂肪酸含量和组成的影响", 《植物资源与环境学报》 * |
| 王亚超 等: "氮、磷等环境因子对太湖微囊藻与水华鱼腥藻生长的影响", 《环境监控与预警》 * |
| 王菁 等: "氮磷比对鱼腥藻和普通小球藻生长竞争的影响", 《江苏农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106479949A (en) * | 2016-09-08 | 2017-03-08 | 上海交通大学 | Activity In Anabaena Azotica cell is by the restorative procedure of ALS inhibitor class herbicide injury |
| CN106479949B (en) * | 2016-09-08 | 2019-12-10 | 上海交通大学 | Method for repairing anabaena azotica cells damaged by ALS inhibitor herbicides |
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