CN105476604A - Method and auxiliary equipment for determining intestinal digestibility of equus animals - Google Patents
Method and auxiliary equipment for determining intestinal digestibility of equus animals Download PDFInfo
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- CN105476604A CN105476604A CN201510936694.4A CN201510936694A CN105476604A CN 105476604 A CN105476604 A CN 105476604A CN 201510936694 A CN201510936694 A CN 201510936694A CN 105476604 A CN105476604 A CN 105476604A
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- auxiliary facilities
- bag
- equus
- intestinal digestibility
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/42—Detecting, measuring or recording for evaluating the gastrointestinal, the endocrine or the exocrine systems
- A61B5/4222—Evaluating particular parts, e.g. particular organs
- A61B5/4255—Intestines, colon or appendix
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- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The invention belongs to the technical field of design and development of animal nutrition experiment methods and equipment and particularly relates to a method and auxiliary equipment for determining the intestinal digestibility of equus animals. The auxiliary equipment comprises a medical nylon bag. Discharging holes are evenly formed in the nylon bag. Steel gaskets are arranged at two ends of the nylon bag. A marking column is arranged on the nylon bag. The method using the auxiliary equipment to determine the intestinal digestibility of the equus animals includes the steps of firstly, preparing the auxiliary equipment; secondly, placing the auxiliary equipment; thirdly, retrieving the auxiliary equipment; fourthly, determining the intestinal digestibility. The method is suitable for the equus animals with cecum fistulas.
Description
Technical field
The invention belongs to the design and R&D technical field of Animal nutrition test method and experimental facilities, particularly relate to a kind of assay method and auxiliary facilities of equus Intestinal digestibility.
Background technology
Be determined as formulation Animal nutrition needs, feeding and management, the animal cultivation etc. of equus Intestinal digestibility provide important evidence.The small intestinal of animal important digests and assimilates place, and Intestinal digestibility is larger on digestibility impact.The assay method of Intestinal digestibility has in vitro method and intracorporal method usually.In vitro method simulates the digestive environments of small intestinal in vitro in experimental provision and simulation preparation artificial digestion liquid digests feedstuff, although in vitro method avoids and carries out surgical operation to animal and cause wound, but real small intestinal digestive environments cannot be createed in vitro, and the difference of the device used and Digestive system composition, be difficult to obtain real Intestinal digestibility.It is that animal installs duodenum fistula that intracorporal method needs by surgical operation, Feed Sample to be measured is sent into small intestinal by duodenum fistula, from excrement, collect mobile Nylon Bag and calculate digestibility, this method does not deduct the microbial digestion effect of large intestine in fact, and the microbial digestion effect of equus large intestine (caecum and colon) on Intestinal digestibility impact greatly, if so do not deduct the microbial digestion effect of large intestine and the digestibility that obtains is digestibility instead of Intestinal digestibility truly.The major defect of intracorporal method is that being necessary for animal installs duodenum fistula, and this affects very large on the health of animal and normal digestion.Therefore traditional in vitro method and intracorporal method all cannot obtain the actual value of equus Intestinal digestibility accurately.
Summary of the invention
Object of the present invention: (1) designs a kind of auxiliary facilities, can directly this equipment be sent in the small intestinal of equus, (2) utilize this auxiliary facilities thus better measure equus Intestinal digestibility, thus improving reliability and the verity of experimental data; Reduce the experimentation cost in Animal nutrition experimental study and labor intensity.
Solve the problems of the technologies described above adopted technical scheme as follows: the auxiliary facilities of (1), mensuration equus Intestinal digestibility, comprise the bag made with medical nylon, uniform on described bag relief hole is set, steel washer is set respectively at bag two ends, bag also arranges labelling hurdle.Described bag is at least made up of the medical nylon of one deck.The long 5-10cm of described bag, wide 0.5-1.5cm.Described bag is long 6cm preferably, wide 1cm.Described bag is provided with engaging lug.Described relief hole aperture 35-40um.The described preferred 38um in relief hole aperture.
(2) adopt above-mentioned auxiliary facilities to the assay method of equus Intestinal digestibility, described assay method comprises the steps:
The first step, the preparation of auxiliary facilities: loaded by feedstuff in auxiliary facilities, seals for subsequent use;
Second step, the input of auxiliary facilities: when equus to be measured search for food raise morning forage volume three/for the moment, auxiliary facilities is delivered in stomach by nasogastric tube;
3rd step, the recovery of auxiliary facilities: put into by caecum fistula Magnet is housed auxiliary facilities for collecting the auxiliary facilities of input; Auxiliary facilities is collected an auxiliary facilities at interval of 1h, is then allowed equus to be measured carry out the walking of 150-250m after throwing in 1-2h; Again collect auxiliary facilities in the manner described above, until stop after 13 hours collecting;
4th step, the mensuration of Intestinal digestibility: the T/A of auxiliary facilities collected in record at every turn; Clean cecal content and the residue on auxiliary facilities surface, freezen protective; Experimental period, terminates to measure the residue in auxiliary facilities afterwards, thus obtains the index result of Intestinal digestibility.Wherein, after preferred auxiliary facilities throws in 1.5h in step 3, start collection work, after each collection, preferably allow equus to be measured carry out the walking of 200m.In step 4, the temperature of freezen protective preferably-20 DEG C.
Beneficial effect of the present invention: measure equus Intestinal digestibility according to method of the present invention, data are reliably true, and experimentation cost is low, and the cycle is short, and labor intensity is low.This method is suitable for the equus being provided with caecum fistula.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is further illustrated.Accompanying drawing 1 is the main TV structure schematic diagram of auxiliary facilities of the present invention.
Detailed description of the invention
The auxiliary facilities of embodiment 1, mensuration equus Intestinal digestibility, as shown in Figure 1, comprise the bag 1 made with medical nylon, uniform on described bag 1 relief hole 2 is set, steel washer 3 is set respectively at bag 1 two ends, bag 1 also arranges labelling hurdle 4.Described bag 1 is at least made up of the medical nylon of one deck.The long 5-10cm of described bag 1, wide 0.5-1.5cm.The preferred long 6cm of described bag 1, wide 1cm.Described bag 1 is provided with engaging lug 5.Described relief hole 2 aperture 35-40um.The preferred 38um in described relief hole 2 aperture.
Embodiment 2, the auxiliary facilities in embodiment 1 is used (in practice, to make Nylon Bag with medical nylon, namely the auxiliary facilities of the following stated refers to Nylon Bag) measure the method for donkey intestinal digestion rate, comprise the steps, the first step, make Nylon Bag, Nylon Bag is of a size of (long 6cm, wide 1cm), nylon cloth aperture is 38um, sample-loading amount is 0.5g(roughage, as Caulis et Folium Oryzae and Herba Medicaginis), 0.7g(concentrated feed is as Semen Maydis, bean cake), heat-seal process, first by three fever sensation of the face envelopes, the unnecessary burr of removing is pruned with shears, then varus avoids scratch intestinal wall to ensure smooth.The two ends that two steel washers (external diameter 10mm) are separately fixed at sack are enclosed in sack.Use permanent marker pen to Nylon Bag numbering (in labelling hurdle), weigh respectively and record after empty bag and dress sample.Second step, the input of Nylon Bag and recovery, after preliminary trial period, terminates (17 days preliminary trial periods) experimental period the first day (8 days experimental periods) that starts, just searched for food after raising morning breakfast three/for the moment, donkey Baoding in Shelf for keeing will be tested, then utilize seal wire to be delivered in stomach by nasogastric tube 45 Nylon Bags.3rd step, the recovery of Nylon Bag, puts into a Nylon Bag that Magnet is housed for collecting Nylon Bag by fistula, and adjustment magnet positions makes it just near going back to blind junction (caecum nipple).After throwing bag 1.5h, take out Magnet and take off the sack collected, then put back to Magnet every 1h, allow experiment donkey carry out the walking of 200m, until take out Magnet after 13 hours to stop closing bag, the sack now received can be used for measuring Intestinal digestibility.All the other sacks are collected in excrement, and are called colostomy bag.4th step, the mensuration of Intestinal digestibility, record receives the T/A of sack at every turn.Wash away cecal content and the residue on Nylon Bag surface with ice-cold tap water, thus cecum microorganisms is stopped action, be placed in-20 DEG C of freezen protective immediately.Each experimental period, terminates rear all sacks and at room temperature thaws, and is then in deionized water and washs with in washing machine, and within general 5 minutes, until rinsing is limpid, at putting into air dry oven 65 DEG C after being classified by sack, general 48h is dried to constant weight.Merge residue in the Nylon Bag from the dress same feedstuff of same head experiment donkey, to obtain sufficient residue.Then residue is ground (by 1mm sieve aperture), be used for measuring DM, CP, ADF, total NSP, NDF, rhamnose, arabinose, xylose, mannose, glucose, alduronic acid.
Claims (8)
1. measure the auxiliary facilities of equus Intestinal digestibility, comprise the bag (1) made with medical nylon, it is characterized in that, described bag (1) is above uniform arranges relief hole (2), steel washer (3) is set respectively at bag (1) two ends, bag (1) also arranges labelling hurdle (4).
2. auxiliary facilities as claimed in claim 1, it is characterized in that, described bag (1) is at least made up of the medical nylon of one deck.
3. auxiliary facilities as claimed in claim 1 or 2, is characterized in that, described bag (1) long 5-10cm, wide 0.5-1.5cm.
4. auxiliary facilities as claimed in claim 3, is characterized in that, the preferred long 6cm of described bag (1), wide 1cm.
5. the auxiliary facilities as described in claim 1,2 or 4, is characterized in that, described bag (1) is provided with engaging lug (5).
6. auxiliary facilities as claimed in claim 1, is characterized in that, described relief hole (2) aperture 35-40um.
7. the auxiliary facilities as described in claim 1 or 6, is characterized in that, the preferred 38um in described relief hole (2) aperture.
8. adopt auxiliary facilities according to claim 1 to the assay method of equus Intestinal digestibility, it is characterized in that, described assay method comprises the steps:
The first step, the preparation of auxiliary facilities: loaded by feedstuff in auxiliary facilities, seals for subsequent use;
Second step, the input of auxiliary facilities: when equus to be measured search for food raise morning forage volume three/for the moment, auxiliary facilities is delivered in stomach by nasogastric tube;
3rd step, the recovery of auxiliary facilities: put into by caecum fistula Magnet is housed auxiliary facilities for collecting the auxiliary facilities of input; Auxiliary facilities is collected an auxiliary facilities at interval of 1h, is then allowed equus to be measured carry out the walking of 150-250m after throwing in 1-2h; Again collect auxiliary facilities in the manner described above, until stop after 13 hours collecting;
4th step, the mensuration of Intestinal digestibility: the T/A of auxiliary facilities collected in record at every turn; Clean cecal content and the residue on auxiliary facilities surface, freezen protective; Experimental period, terminates to measure the residue in auxiliary facilities afterwards, thus obtains the index result of Intestinal digestibility.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510936694.4A CN105476604A (en) | 2015-12-16 | 2015-12-16 | Method and auxiliary equipment for determining intestinal digestibility of equus animals |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510936694.4A CN105476604A (en) | 2015-12-16 | 2015-12-16 | Method and auxiliary equipment for determining intestinal digestibility of equus animals |
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| Publication Number | Publication Date |
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| CN105476604A true CN105476604A (en) | 2016-04-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510936694.4A Pending CN105476604A (en) | 2015-12-16 | 2015-12-16 | Method and auxiliary equipment for determining intestinal digestibility of equus animals |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1756506A (en) * | 2003-03-04 | 2006-04-05 | 奥林巴斯株式会社 | Capsule medical treatment device and capsule medical treatment device collecting system |
| CN101703387A (en) * | 2005-01-06 | 2010-05-12 | G.I.视频有限公司 | Gastrointestinal tool over guiding element |
| CN105050500A (en) * | 2013-03-15 | 2015-11-11 | 马斯公司 | Sampling device |
-
2015
- 2015-12-16 CN CN201510936694.4A patent/CN105476604A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1756506A (en) * | 2003-03-04 | 2006-04-05 | 奥林巴斯株式会社 | Capsule medical treatment device and capsule medical treatment device collecting system |
| CN101703387A (en) * | 2005-01-06 | 2010-05-12 | G.I.视频有限公司 | Gastrointestinal tool over guiding element |
| CN105050500A (en) * | 2013-03-15 | 2015-11-11 | 马斯公司 | Sampling device |
Non-Patent Citations (2)
| Title |
|---|
| 尹召华 等: "用尼龙袋法测定几种牧草在奶牛瘤胃内的降解率", 《黑龙江畜牧兽医》 * |
| 岳群 等: "应用移动尼龙袋法和三步法评定反刍家畜常用饲料的蛋白质小肠消化率", 《中国农业大学学报》 * |
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Application publication date: 20160413 |
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