CN105466722A - A microsampling and processing device and applications - Google Patents
A microsampling and processing device and applications Download PDFInfo
- Publication number
- CN105466722A CN105466722A CN201510799311.3A CN201510799311A CN105466722A CN 105466722 A CN105466722 A CN 105466722A CN 201510799311 A CN201510799311 A CN 201510799311A CN 105466722 A CN105466722 A CN 105466722A
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- CN
- China
- Prior art keywords
- microsampling
- treating apparatus
- stopple coupon
- top cover
- cover cap
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
Abstract
A microsampling and processing device and applications are disclosed. The device is characterized in that the device comprises a top cover plate, at least one top cap disposed on the top cover plate and at least one sampling pipe connected to a corresponding top cap, and the bottom of each sampling pipe is provided with a bottom cap. The device is ingenious in structure design, simple in operation and convenient, and rapid and feasible in subsequent detection and processing. The device is high in practicality and has a good market prospect.
Description
Technical field
The present invention relates to analytical chemistry sample collection and sample preparation association area, particularly relate to a kind of microsampling and treating apparatus and application.
Background technology
Following two kinds of modes are generally had at present: drawn blood by a kind of heparin tube of negative pressure of vacuum, or by the mode referred to of pricking the hand, get blood with kapillary from the detection that performs an analysis of human body blood sample collection.The former needs to be operated by special messenger in hospital, and the blood sample obtained can be tested then and there, also through the transport of long period, can be sent to other places and carry out processing and testing.It is generally then immediately for test that acupuncture finger gets blood, is inconvenient to deposit or transport.For the ease of blood examination, tested individuality, as Disease, the trouble of going to the hospital is removed from when doing blood sample test, oneself gets blood with acupuncture, then blood sample is delivered to mechanism for testing soon to detect, time saving and energy saving province expense, and modern measure instrument and equipment, especially Mass Spectrometer Method instrument, by the mensuration that just can complete most blood sample index or blood drug concentration completely of partly bleeding, existing get that blood mode is still not suitable for existing advanced instrument equipment supporting to facilitate the common people, be more unfavorable for the pre-service that blood sample is necessary before testing.Although technical device has had the development of advancing by leaps and bounds, blood examination still not only inconvenient but also not cheap and most time the blood sample got sheerly unnecessary, actual when detecting with less than 10% wherein even 1%, not only groundless increase sampling object physiology and burden at heart, too increase biological pollution, and the expense that process is polluted still is spread out in it tested object.
Therefore, blood sample that a set of trace of development gets blood and collection is needed in blood examination badly does not need or pre-service easily before detecting.
Summary of the invention
In view of above-mentioned the deficiencies in the prior art, the object of the present invention is to provide a kind of microsampling and treating apparatus and application, be intended to the many deficiencies solving existing blood collecting technology.
Technical scheme of the present invention is as follows:
A kind of microsampling and treating apparatus, wherein, comprise a lamina tecti, at least one the top cover cap be arranged on lamina tecti, be connected at least one stopple coupon of top cover cap, the bottom of described stopple coupon is provided with bottom cap.
Described microsampling and treating apparatus, wherein, described stopple coupon has been arranged side by side multiple, and correspondingly, described top cover cap and bottom cap are provided with multiple.
Described microsampling and treating apparatus, wherein, be filled with adsorbent in described stopple coupon.
Described microsampling and treating apparatus, wherein, described adsorbent is cotton fiber, xylogen, paper fiber, synthon particulate, synthetic resin particulate, ion-exchange particles, carbon black micro mist, silica gel micro mist, chalk soil powder or metal powder.
Described microsampling and treating apparatus, wherein, described top cover cap has the spatial accommodation of 5 ~ 50 microlitres.
Described microsampling and treating apparatus, wherein, described stopple coupon is connected to described top cover cap by folding mode.
Described microsampling and treating apparatus, wherein, the bottom of described stopple coupon is provided with a flow export.
An application for microsampling as above and treating apparatus, wherein, is applied to collected specimens by described microsampling and treating apparatus.
Beneficial effect: microsampling provided by the present invention and treating apparatus, its smart structural design, simple to operate, and when subsequent detection process, convenient and swift easy-to-use, the present invention has higher practicality, good market prospects.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of microsampling of the present invention and treating apparatus first embodiment.
Fig. 2 is the structural representation of a kind of microsampling of the present invention and treating apparatus second embodiment.
Fig. 3 is the decomposition texture schematic diagram of stopple coupon in a kind of microsampling of the present invention and treating apparatus.
Embodiment
The invention provides a kind of microsampling and treating apparatus and application, for making object of the present invention, technical scheme and effect clearly, clearly, the present invention is described in more detail below.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Refer to Fig. 1, a kind of microsampling of the present invention and treating apparatus, it comprises a lamina tecti 100, at least one the top cover cap 110 be arranged on lamina tecti 100, be connected at least one stopple coupon 120 of top cover cap 110, and the bottom of described stopple coupon 120 is provided with bottom cap 130.Described top cover cap 110 can cover the top of described stopple coupon 120.
In the present invention, described stopple coupon 120 can be arranged to single structure as shown in Figure 1, and correspondingly, described top cover cap 110 is provided with 1 with bottom cap 130, thus forms the structure of single stopple coupon.Certainly, described stopple coupon 120 also can be arranged side by side multiple, and correspondingly, described top cover cap 110 is provided with multiple with bottom cap 130, thus forms the structure of connection in a row as shown in Figure 2.
Its shape of top cover cap 110 is wherein cup-shaped, each stopple coupon 120 is all connected to corresponding top cover cap 110, stopple coupon 120 is built with different adsorbents 121, adsorbent 121 can be cotton fiber, xylogen, paper fiber, synthon particulate, synthetic resin particulate, ion-exchange particles, carbon black micro mist, silica gel micro mist (can be various modified silica-gel particulates), chalk soil powder or metal powder etc., or any there is adsorbing material, the material specifically optionally adsorbed and determining.
All top cover caps 110 are all arranged on one piece of lamina tecti 100, are convenient to manual operations.Have the spatial accommodation of 5 ~ 50 microlitres in the middle of top cover cap 110, it can collect blood sample.
Be full of top cover cap 110 when blood sample collection with blood sample floating again, obtain quantitative blood sample.Stopple coupon 120 is connected on lamina tecti 100 by a folding mode; Blood sample is built stopple coupon 120 after filling top cover cap 110 by top cover cap 110; Then stopple coupon 120 is holded up, top cover cap 110, upper, allows the blood sample of 110 li, top cover cap enter stopple coupon 120, and blood sample is diffusion in adsorbent 121 in pipe, and material to be analyzed in blood sample is managed interior adsorbent 121 and adsorbed.Get the microsampling of sample and treating apparatus can deliver to designated analysis laboratory immediately and carry out processing and blood sample analysis.
As shown in Figure 3, be provided with a flow export 122 in the bottom of stopple coupon 120, correspondingly, one groove 131 inserted for flow export 122 can be set at described bottom cap 130 correspondence position.Like this after having got blood sample, the bottom cap 130 of the flow export 122 bottom stopple coupon 120 is pulled up, flow export 122 is inserted a vacuum to take out slip device (not shown) and take out in slip device in vacuum and just place a sample bottle to the below of stopple coupon 120 flow export 122, then top cover cap 110 is opened, solvent or buffer solution is added from the top of stopple coupon 120 with suitable solvent, the amalyzing substances wash-out in stopple coupon 120 out, flow in the sample bottle of below.The sample obtained directly is put in analytical instrument and carries out analytical test.Utilize different solvents or buffer reagent the material of different component in blood sample can also be eluted in different sample bottles respectively to analyze respectively.Stay cell in stopple coupon 120 by adding suitable agent treated, the substance release in cell out, cracking can also be carried out to biotic components such as the albumen in cell, DNA when being necessary, then add suitable solution the composition of needs is eluted in sample bottle, then carry out analysis detection.
After the bottom cap 130 of stopple coupon 120 is pulled up, also the flow export 122 of stopple coupon 120 directly can be inserted a sample injection bottle, then the top of stopple coupon 120 is opened, add suitable solution, sample injection bottle of being ined succession by stopple coupon 120 is again placed on centrifuging inside hydro-extractor together, under the influence of centrifugal force, the filler that the solution that stopple coupon 120 top adds will flow through in stopple coupon 120, elutes inflow sample injection bottle the determinand molecule be adsorbed on filler.
A way is had to be again, the top cover cap 110 of stopple coupon 120 and bottom cap 130 are opened, with one with liquid inlet and and the gland bonnet that links together of an infusion pump and elute soln replace top cover cap 110 by the top being pressed in stopple coupon 120, flow export 122 is connected to a sample injection bottle, start infusion pump and eluent is delivered into stopple coupon 120, the material elutes to be analyzed be adsorbed on wherein filler is flowed into sample injection bottle and is collected.
Aspirin concentrations analysis in embodiment blood
The aspirin tablet of the oral a slice 100mg of experimental subjects, afterwards at the time point blood sample collection of 5,15,30,60,90,120,180,240,360,480 minutes.Pricked on finger with the blood pin of getting can bought on the market before this when getting blood at every turn, wound is pressed in gently on the edge of the top cover cap 110 of 20-microlitre of the present invention, blood is allowed to flow in top cover cap 110, when top cover cap 110 installs to fully completely, with the finger getting blood gently along top cover cap 110 periphery flat wiping once, make blood sample liquid level just concordant with top cover cap 110 surface, then stopple coupon 120 clinching is made it to cover tightly reality on top cover cap 110, then stopple coupon 120 is just being erected, blood sample is allowed to flow into stopple coupon 120, now can suitably stopple coupon 120 bottom be struck on desk several under, blood sample is allowed to enter into stopple coupon 120 completely.Microsampling and treating apparatus are delivered to assay laboratory after getting by the blood sample of all 10 time points, carry out sample preparation and analyze measuring.The stopple coupon 120 used in the present embodiment is filled with degreasing cotton fiber, methyl alcohol is added at stopple coupon 120 top during process, the flow export 122 of bottom inserts sample bottle, allow rear centrifuging wash-out, cell in blood sample and albumen and lipid material are stayed in stopple coupon 120, and aspirin molecule is eluted in sample bottle and carries out concentration determination as analysis sample.The sample liquid chromatograph/mass spectrometer obtained carries out quantitative test.The result obtained is as shown in table 1 below:
Table 1
| Sampling spot (minute) | 30 | 60 | 90 | 120 | 180 | 240 | 360 | 480 | 720 | 1440 |
| Concentration (nanograms/milliliter) | 67.5 | 405 | 1030 | 1950 | 3140 | 3870 | 3590 | 2850 | 2030 | 371 |
The present invention also provides the application of a kind of microsampling as above and treating apparatus, and described microsampling and treating apparatus are applied to collected specimens.
Should be understood that, application of the present invention is not limited to above-mentioned citing, for those of ordinary skills, can be improved according to the above description or convert, and all these improve and convert the protection domain that all should belong to claims of the present invention.
Claims (8)
1. microsampling and a treating apparatus, is characterized in that, comprise a lamina tecti, at least one the top cover cap be arranged on lamina tecti, be connected at least one stopple coupon of top cover cap, the bottom of described stopple coupon is provided with bottom cap.
2. microsampling according to claim 1 and treating apparatus, is characterized in that, described stopple coupon has been arranged side by side multiple, and correspondingly, described top cover cap and bottom cap are provided with multiple.
3. microsampling according to claim 1 and treating apparatus, is characterized in that, is filled with adsorbent in described stopple coupon.
4. microsampling according to claim 3 and treating apparatus, it is characterized in that, described adsorbent is cotton fiber, xylogen, paper fiber, synthon particulate, synthetic resin particulate, ion-exchange particles, carbon black micro mist, silica gel micro mist, chalk soil powder or metal powder.
5. microsampling according to claim 1 and treating apparatus, is characterized in that, described top cover cap has the spatial accommodation of 5 ~ 50 microlitres.
6. microsampling according to claim 1 and treating apparatus, is characterized in that, described stopple coupon is connected to described top cover cap by folding mode.
7. microsampling according to claim 1 and treating apparatus, is characterized in that, the bottom of described stopple coupon is provided with a flow export.
8. an application for microsampling as claimed in claim 1 and treating apparatus, is characterized in that, described microsampling and treating apparatus are applied to collected specimens.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510799311.3A CN105466722A (en) | 2015-11-18 | 2015-11-18 | A microsampling and processing device and applications |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510799311.3A CN105466722A (en) | 2015-11-18 | 2015-11-18 | A microsampling and processing device and applications |
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| CN105466722A true CN105466722A (en) | 2016-04-06 |
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| CN201510799311.3A Pending CN105466722A (en) | 2015-11-18 | 2015-11-18 | A microsampling and processing device and applications |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004184099A (en) * | 2002-11-29 | 2004-07-02 | I Design:Kk | Blood collecting tool |
| JP2005218677A (en) * | 2004-02-06 | 2005-08-18 | Enomoto Co Ltd | Quantitative blood sampling kit |
| CN1780583A (en) * | 2003-04-25 | 2006-05-31 | 积水化学工业株式会社 | Method and tool for filtrating specimen using specimen sampling container, and specimen sampling container |
| CN1910455A (en) * | 2003-12-24 | 2007-02-07 | 贝克顿·迪金森公司 | Plasma on demand tube |
| CN101420902A (en) * | 2006-02-08 | 2009-04-29 | 贝克顿·迪金森公司 | Blood collection device, method, and system for using the same |
| US7740804B2 (en) * | 2005-04-12 | 2010-06-22 | Chromedx Inc. | Spectroscopic sample holder |
| CN102946803A (en) * | 2010-06-24 | 2013-02-27 | 伊莱克有限公司 | Blood collection module for measuring alcohol concentration |
| CN104042253A (en) * | 2013-03-15 | 2014-09-17 | 奥索临床诊断有限公司 | Rotatable disk-shaped fluid sample collection device |
| WO2014145330A2 (en) * | 2013-03-15 | 2014-09-18 | Theranos, Inc. | Methods and devices for sample collection and sample separation |
-
2015
- 2015-11-18 CN CN201510799311.3A patent/CN105466722A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004184099A (en) * | 2002-11-29 | 2004-07-02 | I Design:Kk | Blood collecting tool |
| CN1780583A (en) * | 2003-04-25 | 2006-05-31 | 积水化学工业株式会社 | Method and tool for filtrating specimen using specimen sampling container, and specimen sampling container |
| CN1910455A (en) * | 2003-12-24 | 2007-02-07 | 贝克顿·迪金森公司 | Plasma on demand tube |
| JP2005218677A (en) * | 2004-02-06 | 2005-08-18 | Enomoto Co Ltd | Quantitative blood sampling kit |
| US7740804B2 (en) * | 2005-04-12 | 2010-06-22 | Chromedx Inc. | Spectroscopic sample holder |
| CN101420902A (en) * | 2006-02-08 | 2009-04-29 | 贝克顿·迪金森公司 | Blood collection device, method, and system for using the same |
| CN102946803A (en) * | 2010-06-24 | 2013-02-27 | 伊莱克有限公司 | Blood collection module for measuring alcohol concentration |
| CN104042253A (en) * | 2013-03-15 | 2014-09-17 | 奥索临床诊断有限公司 | Rotatable disk-shaped fluid sample collection device |
| WO2014145330A2 (en) * | 2013-03-15 | 2014-09-18 | Theranos, Inc. | Methods and devices for sample collection and sample separation |
| CN105209880A (en) * | 2013-03-15 | 2015-12-30 | 赛拉诺斯股份有限公司 | Methods and devices for sample collection and sample separation |
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Application publication date: 20160406 |