CN105462834A - Tumor cell capturing micro-fluidic chip and tumor cell capturing method - Google Patents
Tumor cell capturing micro-fluidic chip and tumor cell capturing method Download PDFInfo
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Abstract
The invention discloses a tumor cell capturing micro-fluidic chip. The tumor cell capturing micro-fluidic chip comprises a base material and a microchannel formed inside the base material, wherein the microchannel extends along a smooth curve, an inlet and an outlet are formed in the two ends of the microchannel respectively, a microcolumn array is arranged in the vertical direction of the interior of the microchannel in a protruding mode, the microcolumn array is distributed in the extending direction of the microchannel, the end, located at the inlet, of the microcolumn array is attached to one side wall of the microchannel, and the end, located at the outlet, of the microcolumn array is attached to the other side wall of the microchannel; capture cavities with triangular cross sections are distributed in the middle of the microcolumn array at equal intervals into an array, and the three vertex angles of each capture cavity form three gaps which are 5 microns wide to be used for capturing one tumor cell 2-3 times. The chip can be used for capturing EpCAM expressed and unexpressed CTCs, EpCAM unexpressed and low-expressed CTCs and CTCs not connected with magnetic beads by means of the microcolumn array with 5 microns wide gaps.
Description
Technical field
The application relates to a kind of tumour cell and catches micro-fluidic chip and tumour cell catching method, and the micro-fluidic chip that can be applicable to all kinds circulating tumor cell is caught, and belongs to biophysics field.
Background technology
Cancer, has another name called malignant tumour.It is one of first cause causing human death in the world.Be harm humans life and health, cause the first foul disease of modern humans's death and chronic disease.The cancer patient of 90% dies from metastasis of cancer, and capturing cancer is the target that the mankind seek assiduously.Traditional operation, chemotherapy, the method cost of radiotherapy is high, complicated, brings larger misery and have recurrent to patient.Circulating tumor cell (CTCs) comes off from primary knurl, enters the tumour cell in blood circulation.These tumour cells can flow to other positions and grow and grow, and develop into new knurl.Detect in blood circulation that circulating tumor cell means cancer and possible metastasis of cancer.The number of CTCs and the severity of cancer have close contacting.Number is many, is in a bad way, and the few state of an illness of number is light or treatment is effective.
The microchannel of micro-fluidic chip is suitable with the size of cell, is very suitable for the research of cell capture.Micro-fluidic chip is considered to a kind of new diagnostic instrument of Noninvasive.The existing micro-fluidic chip that utilizes studies catching separation method and substantially can being divided into two classes of CTCs:
One class utilizes affinity (Affinityreaction) principle carry out catching of cell and be separated, the method depends on modification can be combined with cell-surface antigens on the microchannel or microstructure of micro-fluidic chip inside specific antibody if anti-EpCAM is as CTC-chip, recently it is fish scale shape that the fish scale core sheet that the micro-whirlpool improved produces changes micro-pillar array, improves the pillar probability of collision of cell and antibody modification.Improve capture rate and purity.But the structure of this micro-fluidic chip is usually very complicated, the time of modifying is normal, and antibody used is expensive.And in different tumour cells, the expression of EpCAM is had nothing in common with each other.And when tumour cell generation Epithelial and stromal transforms (EMT), the expression of the EpCAM of tumour cell reduces.Therefore depend on the method catch CTCs likely can lose a part do not express or low expression EpCAM tumour cell.This catching method depends on the probability of collision of the pillar of cell and antibody modification.When accelerating flow velocity, the probability of collision of cell and antibody modification pillar reduces and interactional time decreased, and capture rate declines.Typical microfluidic chip structure has CTC-chip, HB-Chip, HTMSU, Onco-BeanChip.
Another kind of is that physically based deformation characteristic such as size deformation (ISET:isolationbysizeofepithelialtumorcells) is caught, and utilizes CTCs larger than hemocyte and the feature of not easily deformation, filters hemocyte.The method is simple to operate, and structure does not need too complicated, does not need to modify, does not rely on the mark on any surface.But because CTCs has overlapping part with leukocytic size, so a part of CTCs may pass through filter screen or microtrabeculae gap.A part may be lost and white corpuscle size has the CTCs of lap, the CTCs that especially those sizes are less.Because experienced by the transformation of EMT, so the pernicious degree of the CTCs missed is higher.And it is not high with purity easily to break.The easy phenomenon that blocking occurs.But with affinity or other are as compared with the methods such as two-dimensional electrophoresis, the method is simple and easy, practical, is more suitable for clinical application.
Design the requirement that micro-fluidic chip just can meet desirable CTCs detection platform fine.(1) hypersensitivity: each CTC can be detected.1-100 tumour cell is only had but to have 10 in the blood of 1ml
7individual white corpuscle and 10
9individual red corpuscle.So detect that each tumour cell in blood sample has vital meaning; (2) high purity: the CTCs of separation does not have other cell as the hemocyte such as white corpuscle and red corpuscle, is more conducive to detection and indentification; (3) high-throughput: in order to separate tumour cell as much as possible to cultivate and molecular biological analyses, clinical middle used blood sample is 7.5ml, efficiently isolates tumour cell rapidly to meet clinical demand; (4) keep the activity of cell and integrity: catch washing of rear tumour cell and release, active, biological genetic analysis with hatch the further analysis be conducive to patient's state of an illness, Detection and Identification.Uniquely be applied to the clinical CellSearch obtaining U.S. FDA certification only having capture rate not high at present.Only be confined to mammary cancer, colorectal cancer and prostate cancer.The also false positive results caused by epithelium expression of possible non-epithelial cell or non-neoplastic epithelial cells.The method also exists semi-automatic, high cost, inefficient defect.
Summary of the invention
A kind of tumour cell is the object of the present invention is to provide to catch micro-fluidic chip and tumour cell catching method, to overcome deficiency of the prior art.
For achieving the above object, the invention provides following technical scheme:
The embodiment of the present application discloses a kind of tumour cell and catches micro-fluidic chip, comprise base material, and the microchannel be formed in described base material, this microchannel extends along smooth curve direction, its two ends are formed with entrance and exit respectively, vertically micro-pillar array has been protruded out in described microchannel, this micro-pillar array is along the bearing of trend array distribution of described microchannel, its one end being positioned at ingress fits in a sidewall of described microchannel, its one end being positioned at exit fits in another sidewall of described microchannel, what the equidistant array distribution in middle part of described micro-pillar array had a triangular cross section catches cavity, these three drift angles of catching cavity form the gap of three 5 microns respectively, catching for 2-3 time for a tumour cell.
Preferably, catch in micro-fluidic chip at above-mentioned tumour cell, the spacing in micro-pillar array between each microtrabeculae is 5 microns, and the spacing between trapezoidal microtrabeculae and between trapezoidal and micro-cylinder is 5 microns.
The size of tumour cell between 10-20 μm, red corpuscle 4-6 μm, white corpuscle 7-12 μm.This size meets the diameter being less than tumour cell, and is the experimental verification in a lot of document.
Preferably, catch in micro-fluidic chip at above-mentioned tumour cell, described micro-pillar array comprises the trapezoidal micro-pillar array and cylindrical micro-pillars array that are set up in parallel, described trapezoidal micro-pillar array comprises along the equidistant array distribution of microchannel bearing of trend and has the multiple trapezoidal microtrabeculae of trapezoidal cross-section, described cylindrical micro-pillars array comprises along the equidistant array distribution of microchannel bearing of trend and has multiple cylindrical micro-pillars of circular cross section, described trapezoidal micro-pillar array and cylindrical micro-pillars array parallel are arranged, adjacent 2 described trapezoidal microtrabeculaes and 1 cylindrical micro-pillars surround there is described in one triangular cross section catch cavity.
Preferably, catch in micro-fluidic chip at above-mentioned tumour cell, described microchannel outwards distributes twist from entrance.
Preferably, catch in micro-fluidic chip at above-mentioned tumour cell, spiral described microchannel has 4 circles.
Preferably, catch in micro-fluidic chip at above-mentioned tumour cell, described microchannel comprises multiple helical passage, and the plurality of passage has same entrance, and multiple passage is in being alternately distributed.
Preferably, catch in micro-fluidic chip at above-mentioned tumour cell, the distance between the microchannel sidewall corresponding thereto of the long limit of the described trapezoidal micro-pillar array of interlude is 80 μm.
Preferably, catch in micro-fluidic chip at above-mentioned tumour cell, the distance between the described cylindrical micro-pillars array microchannel sidewall corresponding thereto of interlude is 120 μm.
Preferably, catch in micro-fluidic chip at above-mentioned tumour cell, the below of described base material is provided with permanent magnet, is provided with alnico magnets when catching, and removes alnico magnets during enrichment.。
Accordingly, disclosed herein as well is a kind of method of catching tumour cell, comprise: the entrance of blood sample from micro-fluidic chip is injected, in this blood sample, tumour cell has hatched magnetic bead in advance, under the effect of high-intensity magnetic field, blood sample is by the inner side of micro-pillar array in microchannel, filtration is caught by trapezoidal and cylindrical micro-pillars array, flow away from the blank position outside microchannel, simultaneously, the magnetic tumour cell of tool is attracted in the bottom surface of micro-fluidic chip by high-intensity magnetic field, stick on chip, the drill-through micro-pillar array of hemocyte, flow away from the blank position outside microchannel, after catching, pass into phosphate buffered saline buffer to rinse, during enrichment, remove alnico magnets, magnetic disappears, first closed one end outlet, rinse to the center of circle and ingress from another exit with nutrient solution or damping fluid, the tumour cell of this microchannel IT can be rinsed, then this outlet is closed, another outlet is opened, rinse to circle centre position from another exit with nutrient solution or damping fluid, the tumour cell of another passage IT can be rinsed, realize enrichment.
Compared with prior art, the invention has the advantages that:
1), chip of the present invention is easy to operate, passed into by blood sample, can realize the separation of whole blood from circle centre position;
2), chip of the present invention has high capture rate, combines 2 kinds of catching methods simultaneously, catches for 3 times of 2 layers of micro-pillar array and ensure that high capture rate.
3), chip of the present invention is practically applicable to express EpCAM and do not express the CTCs of EpCAM, do not express and the micro-pillar array of 5 micron pitch can be utilized to catch with the CTCs of low expression EpCAM and the CTCs that is not connected upper magnetic bead.
4), high-throughput, comparatively slight drag when circular and spiral structure guarantees that blood sample flows, blood sample realizes quickly and efficiently being separated in chip.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present application or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, the accompanying drawing that the following describes is only some embodiments recorded in the application, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Figure 1 shows that the vertical view of micro-fluidic chip in the specific embodiment of the invention;
Figure 2 shows that the enlarged diagram of microchannel in the specific embodiment of the invention;
Figure 3 shows that the perspective view of micro-fluidic chip in the specific embodiment of the invention;
Figure 4 shows that the outside surface of tumour cell connects the photo of the upper magnetic immunomagnetic beads of tool;
Figure 5 shows that the photo of each the magnetic immunomagnetic beads of connection tool outside a tumour cell;
Figure 6 shows that the photo of micro-fluidic chip capture effect in the specific embodiment of the invention;
Embodiment
The present embodiment provides a kind of micro-fluidic chip, can realize the detection of the clinical blood sample of 7.5ml, and nearly 4 circles of this chip each round screw thread shape, are embedded in mutually together.In the round screw thread shape microchannel of this micro-fluidic chip, the cylindrical micro-pillars array parallel with this trapezoidal micro-pillar array with by trapezoidal micro-pillar array forms, and two spacing of going to the bottom between adjacent two corner angle of trapezoidal micro-pillar array are 5 microns.Micro-cylinder is positioned at the gap location that adjacent two micro-trapezoidal upper bases are formed, and the spacing between two corner angle of two micro-trapezoidal upper bases is also 5 microns.This two-layer parallel micro-pillar array is located substantially on the middle position of microchannel.The home position place be connected of two round screw thread shape micro-pillar array is entrances, and respectively there is an outlet both sides, outer end, left and right.Two-layer parallel micro-pillar array is connected with the outside of passage in ingress, is connected with the inner side of passage in exit.
Described chip its will catch, separation and enrichment cycles tumour cell (CTCs) are integrated in one, this chip easy handling, do not rely on the marker of tumour cell, be not limited to tumour cell and whether express epithelial adherence molecule (EpCAM), be divided into two kinds, that expresses and do not express the circulating tumor cell of EpCAM catches test, for the circulating tumor cell of expressing EpCAM (epithelial adherence molecule), by alnico magnets very strong for one piece of magnetic being placed in the below of micro-fluidic chip, high-intensity magnetic field can be produced on the surface of micro-fluidic chip, by circulating tumor cell bag by the magnetic immunomagnetic beads of tool, blood sample is passed at circle centre position, when blood sample flows in two round screw thread shape passages nested against one another, caught by the micro-pillar array based on size on the one hand, and on the other hand, under the effect of high-intensity magnetic field, connect immunomagnetic beads and the magnetic tumour cell of tool under the effect of high-intensity magnetic field, be attracted to the bottom of micro-fluidic chip, the size of tumour cell and the dual of magnetic of the expression EpCAM that this chip achieves catch.And for not expressing the tumour cell of EpCAM, then can directly blood sample be passed into from ingress, the center of circle, the gap location of tumour cell micro-pillar array in round screw thread shape passage is captured.White corpuscle and red corpuscle then flow away by micro-pillar array.During enrichment, remove magnet, magnetic field dissipate, from exit nutrient solution or wash buffer, captured tumour cell can be rinsed, realize enrichment.
Micro-pillar array in micro-fluidic chip can realize catching of size effectively, or dually catching of being combined with Magnetic Phase of size.The design of circle and helix structure, various resistances when can effectively avoid fluid to flow, the flowing that blood sample can be unobstructed in microchannel, fast and effeciently realizes the separation of circulating tumor cell.
Micro-fluidic chip expresses the acquisition mode of the circulating tumor cell of EpCAM, and the magnetic adopting high-intensity magnetic field to produce and the dual of size catch, and remove magnet, magnetic field dissipate, realize enrichment.For the acquisition mode of circulating tumor cell not expressing EpCAM, then catching by means of size, during enrichment, rinses from exit to ingress, the CTCs caught can be washed and release, realize enrichment.The passage width of 230 microns can realize catching the cultivation on rear tumour cell chip simultaneously.This chip also can realize the detection of 2 blood samples simultaneously, and first a closed outlet, injects blood sample from entrance, flow out from one of them spirane structure; Then by closed for another outlet, this outlet opened, inject another blood sample from entrance, this blood sample realizes catching in another helical channel.Described chip can realize the detection of chip piece 2 samples.
Below in conjunction with the accompanying drawing in the embodiment of the present invention, be described in detail the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art obtain under the prerequisite not making creative work, all belongs to the scope of protection of the invention.
Shown in composition graphs 1, tumour cell catches micro-fluidic chip, comprise base material, and the microchannel 5 be formed in base material, this microchannel extends along smooth curve direction, its two ends are formed with entrance 1 and outlet 2 respectively, vertically micro-pillar array has been protruded out in microchannel, this micro-pillar array is along the bearing of trend array distribution of microchannel, its one end being positioned at ingress fits in an outer side wall of microchannel, its one end being positioned at exit fits in another inner side-wall of microchannel, what the equidistant array distribution in middle part of micro-pillar array had a triangular cross section catches cavity, these three drift angles of catching cavity form the gap of three 5 microns respectively, catching for 2-3 time for a tumour cell.。
In a preferred embodiment, for catching the micro-fluidic chip of tumour cell primarily of the structure formation nested against one another of 4 circles, two round screw thread shapes, can be made by PDMS (polydimethylsiloxane).Micro-fluidic chip entrance 1, is positioned at home position place, and left and right respectively connects a circle and volution microchannel.The outlet 2 of micro-fluidic chip, is each positioned at outside about micro-fluidic chip.Micro-column structure 3 trapezoidal in passage, upper base 20 microns, goes to the bottom 40 microns, high 30 microns.This micro-pillar array is substantially parallel with microchannel, in the outside near entry position place and microchannel to being connected, is being connected with the inner side of microchannel in close exit.
Utilize above-mentioned micro-fluidic chip to catch the method for circulating tumor cell, this catching method is divided into two kinds, and that expresses and do not express the circulating tumor cell of EpCAM catches test; For the circulating tumor cell of expressing EpCAM (epithelial adherence molecule), by circulating tumor cell bag by the magnetic immunomagnetic beads of tool, and place the very strong permanent magnet of one piece of magnetic in the below of micro-fluidic chip, the magnetic field that magnetic is very strong is produced in micro-fluidic chip, when blood sample injects entrance 1, under the effect of high-intensity magnetic field, blood sample is by the inner side of microtrabeculae in microchannel, caught filtration 3 times by trapezoidal and cylindrical micro-pillars array, flow away from the blank position outside microchannel.Meanwhile, the magnetic CTCs of tool is attracted in the bottom surface of micro-fluidic chip by high-intensity magnetic field, sticks on chip.Hemocyte is if white corpuscle and red corpuscle are then due to small size, and the feature of easy deformation can drill-through micro-pillar array, flows away from the blank position outside microchannel.After catching, pass into phosphate buffered saline buffer (PBS) and rinse, the purity of catching can be improved.During enrichment, remove magnet, magnetic disappears, first the outlet of closed one end, rinses to the center of circle and ingress from another exit with nutrient solution or damping fluid, the tumour cell of this microchannel IT can be rinsed, then this outlet is closed, another outlet is opened, rinses to circle centre position with nutrient solution or damping fluid from another exit, the tumour cell of another passage IT can be rinsed, realize enrichment.For the tumour cell of not expressing EpCAM or low expression EpCAM, then do not connect immunomagnetic beads, directly utilize size and deformation to catch.
Shown in ginseng Fig. 2, due in microchannel, the spacing 6 in micro-pillar array front is 80 microns, and the spacing 7 at micro-pillar array rear is 120 microns, so this round screw thread shape micro-fluidic chip can realize high-throughput, meets the demand of clinical 7.5ml.Enough spaces realize the cultivation on the tumour cell chip of catching.
Fig. 3 is the structure diagram schematic diagram of this two channels round screw thread shape micro-fluidic chip, the tendency of micro-pillar array as can be seen from the figure.
Whether this micro-fluidic chip successfully can depend on that circulating tumor cell connects the efficiency of immunomagnetic beads to a large extent.If all CTCs can connect the immunomagnetic beads of diameter 4.5 microns, and the immunomagnetic beads that each tumour cell connects is more, just can CTCs be made to have magnetic stronger, attracted the possibility on micro-fluidic chip bottom surface larger in the effect of high-intensity magnetic field by the effect of high-intensity magnetic field, the size of tumour cell is amplified simultaneously, the successful of magnetic catch can be guaranteed further.Be the picture that breast cancer cell MCF-7 connects magnetic immuno magnetic bead shown in Fig. 5, in this picture, all tumour cells have all connected the magnetic immuno magnetic bead of diameter 4.5 microns.Make tumour cell have magnetic and be exaggerated 4.5 microns-9 microns.Guarantee effective enforcement of 2 kinds of catching methods.The method that CTCs connects magnetic immuno magnetic bead implements also very simple, and in the tumor cell suspension of 1ml, add the immunomagnetic beads of 25 microlitres, turned upside down within half an hour this cell suspension of mixing, can obtain the effect as Fig. 4.
Empirical tests, the CTCs of nearly all expression EpCAM the connection of nearly 100% can be with magnetic immunomagnetic beads within half an hour, only can realize catching of CTCs by magnetic.
Fig. 5 is that a tumour cell connects the magnetic immunomagnetic beads of tool, connects the picture that effect is very good.In this picture, breast cancer cell MCF-7 is the magnetic immunomagnetic beads of tool on each orientation all connects.Thus effectively guarantee the success of magnetic catch.And the size of this tumour cell is effectively exaggerated 9 microns, ensure that very big that size is caught may.
Fig. 6 is the capture effect picture of this micro-fluidic chip, can find out that from this picture the tumour cell having contaminated look is trapped on chip, and in microchannel survey, in the outside of this microchannel without any tumour cell, confirm the validity of this micro-fluidic chip when catching.
It should be noted that, in this article, the such as relational terms of first and second grades and so on is only used for an entity or operation to separate with another entity or operational zone, and not necessarily requires or imply the relation that there is any this reality between these entities or operation or sequentially.And, term " comprises ", " comprising " or its any other variant are intended to contain comprising of nonexcludability, thus make to comprise the process of a series of key element, method, article or equipment and not only comprise those key elements, but also comprise other key elements clearly do not listed, or also comprise by the intrinsic key element of this process, method, article or equipment.When not more restrictions, the key element limited by statement " comprising ... ", and be not precluded within process, method, article or the equipment comprising described key element and also there is other identical element.
The above is only the embodiment of the application; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the application's principle; can also make some improvements and modifications, these improvements and modifications also should be considered as the protection domain of the application.
Claims (10)
1. a tumour cell catches micro-fluidic chip, it is characterized in that, comprise base material, and the microchannel be formed in described base material, this microchannel extends along smooth curve direction, its two ends are formed with entrance and exit respectively, vertically micro-pillar array has been protruded out in described microchannel, this micro-pillar array is along the bearing of trend array distribution of described microchannel, its one end being positioned at ingress fits in a sidewall of described microchannel, its one end being positioned at exit fits in another sidewall of described microchannel, what the equidistant array distribution in middle part of described micro-pillar array had a triangular cross section catches cavity, these three drift angles of catching cavity are formed and the described opening of catching cavity and being communicated with respectively.
2. tumour cell according to claim 1 catches micro-fluidic chip, it is characterized in that: the width of described opening is less than the diameter of tumour cell.
3. tumour cell according to claim 2 catches micro-fluidic chip, it is characterized in that: described micro-pillar array comprises the trapezoidal micro-pillar array and cylindrical micro-pillars array that are set up in parallel, described trapezoidal micro-pillar array comprises along the equidistant array distribution of microchannel bearing of trend and has the multiple trapezoidal microtrabeculae of trapezoidal cross-section, described cylindrical micro-pillars array comprises along the equidistant array distribution of microchannel bearing of trend and has multiple cylindrical micro-pillars of circular cross section, described trapezoidal micro-pillar array and cylindrical micro-pillars array parallel are arranged, adjacent 2 described trapezoidal microtrabeculaes and 1 cylindrical micro-pillars surround there is described in one triangular cross section catch cavity, these three drift angles of catching cavity form the described opening of three 5 microns respectively, catching for 2-3 time for a tumour cell.
4. tumour cell according to claim 1 catches micro-fluidic chip, it is characterized in that: described microchannel outwards distributes twist from entrance.
5. tumour cell according to claim 4 catches micro-fluidic chip, it is characterized in that: spiral described microchannel has 4 circles.
6. the tumour cell according to claim 4 or 5 catches micro-fluidic chip, it is characterized in that: described microchannel comprises multiple helical passage, and the plurality of passage has same entrance, and multiple passage is in being alternately distributed.
7. tumour cell according to claim 1 catches micro-fluidic chip, it is characterized in that: the distance between the microchannel sidewall corresponding thereto of the long limit of the described trapezoidal micro-pillar array of interlude is 80 μm.
8. tumour cell according to claim 1 catches micro-fluidic chip, it is characterized in that: the distance between the described cylindrical micro-pillars array microchannel sidewall corresponding thereto of interlude is 120 μm.
9. tumour cell according to claim 1 catches micro-fluidic chip, it is characterized in that: the below of described base material is provided with places and the alnico magnets removed when enrichment when catching.
10. tumour cell according to claim 9 catches the method that micro-fluidic chip catches tumour cell, it is characterized in that, comprise: the entrance of blood sample from micro-fluidic chip is injected, in this blood sample, tumour cell has hatched magnetic bead in advance, under the effect of high-intensity magnetic field, blood sample is by the inner side of micro-pillar array in microchannel, filtration is caught by trapezoidal and cylindrical micro-pillars array, flow away from the blank position outside microchannel, simultaneously, the magnetic tumour cell of tool is attracted in the bottom surface of micro-fluidic chip by high-intensity magnetic field, stick on chip, the drill-through micro-pillar array of hemocyte, flow away from the blank position outside microchannel, after catching, pass into phosphate buffered saline buffer to rinse, during enrichment, remove alnico magnets, magnetic disappears, first closed one end outlet, rinse to the center of circle and ingress from another exit with nutrient solution or damping fluid, the tumour cell of this microchannel IT can be rinsed, then this outlet is closed, another outlet is opened, rinse to circle centre position from another exit with nutrient solution or damping fluid, the tumour cell of another passage IT can be rinsed, realize enrichment.
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| CN107233941A (en) * | 2017-04-24 | 2017-10-10 | 深圳无微华斯生物科技有限公司 | A kind of multiple near-infrared fluorescent enhancing biochip screening circulating tumor cell method |
| CN108663258A (en) * | 2017-03-29 | 2018-10-16 | 苏州含光微纳科技有限公司 | A kind of circulating tumor cell screening chip and method |
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| CN110272811A (en) * | 2019-07-05 | 2019-09-24 | 大连海事大学 | A kind of unicellular surface portion region magnetizing assembly and method based on twin columns capture |
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| CN113188980B (en) * | 2021-04-28 | 2022-09-16 | 南通大学 | Whole blood circulating tumor cell cascade sorting device and method based on fluorescence activated cell sorting technology |
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Address after: 215000 No. 128, Fang Zhou road, Suzhou Industrial Park, Jiangsu, China Patentee after: SUZHOU WENHAO MICROFLUIDIC TECHNOLOGY Co.,Ltd. Address before: 215808 No. 128, Fang Zhou road, Suzhou Industrial Park, Jiangsu, China Patentee before: SUZHOU WENHAO CHIP TECHNOLOGY Co.,Ltd. |