CN105454304B - Ginkgolides is as the application in fall webworms larva anti-feedant - Google Patents
Ginkgolides is as the application in fall webworms larva anti-feedant Download PDFInfo
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- CN105454304B CN105454304B CN201510863812.3A CN201510863812A CN105454304B CN 105454304 B CN105454304 B CN 105454304B CN 201510863812 A CN201510863812 A CN 201510863812A CN 105454304 B CN105454304 B CN 105454304B
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- ginkgolides
- fall webworms
- ginkgo biloba
- feedant
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
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- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pest Control & Pesticides (AREA)
- Medicines Containing Plant Substances (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to ginkgo biloba p.es as the application in fall webworms larva anti-feedant.The ginkgo biloba p.e is following at least one：Ginkgolides, Bilobalide, ginkolide B, ginkalide A and ginkgo biloba crude extract.The study find that new application of the ginkgo biloba p.e in terms of pest control, provides a kind of efficient Plant antifeedant, the harm for preventing fall webworms larva.Plant antifeedant is native compound, can be easily decomposed in the natural environment, less environmental pollution, has higher environmental benefit.
Description
Technical field
Present invention relates particularly to ginkgolides as the application in fall webworms larva anti-feedant.
Background technology
Fall webworms originate in North America, belong to Lepidoptera, Arctiidae, the entitled Hyphantriacunea of Latin.Fall webworms exist
In 2-3 generations, occur for the general 1a in China, overwintering with pupa.In June, 1979, towards border area diseases and pests of agronomic crop tune in Liaoning Province of China
It is found for the first time when looking into, the provinces and cities such as wide-scale distribution to Shaanxi, Tianjin, Shanghai, Liaoning, Hebei, Shandong, Beijing.
Fall webworms larva feeding habits are miscellaneous, and breeding amount is big, adaptable, and route of transmission is wide, endanger a variety of forests and fruit tree, tree
Leaf eats up crops, vegetables and the wild plant near rear just harm.In China, the host through preliminary investigation fall webworms has 94
Kind, be under the jurisdiction of 37 sections, wherein Chinese wax maple and mulberry are aggrieved the most serious, followed by alder, oriental cherry, apple, hawthorn, Malus spectabilis, Lee,
Eugene poplar, populus simonii etc..
Fall webworms larva mainly by timber, propagated by wooden packing etc., can also pass through further diffusion of circling in the air.Its is numerous
It is strong to grow power, diffusion is fast, every year can be to external diffusion 35-50km.Fruit tree, forest, more than 200 kinds of crops and wild plant etc. can be endangered
Plant, in the place and excursion district that orchard is intensive, complete stool leaf can be eaten light when occurring serious, cause part branch by boulevard
The even whole strain of item is dead.Sericulture, industry of planting forest or fruit tress and urban afforestation are seriously threatened, serious economic loss is caused.
Ginkgo leaf, which is used as medicine, starts from the Ming Dynasty.Civil available ginkgo leaf treats senile angiocardiopathy.After the seventies, moral, method
Equal European countries have successively carried out the research to ginkgo leaf.German Schwade drugmakers patent in 1991 produces ginkgo leaf
Standard extract EGb761 (Extracts of Gingkgo Biloba), quality index are flavonoid glycoside >=24%, terpene lactones
>=6%.
Terpene lactones compound is that another kind of important bioactive compound, Determination of ginkgolides are in ginkgo leaf
5%-7% at present it is separated go out 6 kinds of terpene lactones, be referred to as ginkgolides.Ginkgolides of Ginkgo Leaf is diterpene and sesquiterpenoids chemical combination
Object.It is isolated from ginkgo leaf and skin, 5 diterpene-kind compounds are shared, in ginkalide A, ginkolide B, ginkgo
Ester C, bilobalide J and ginkgolides M, wherein ginkgolides M are separated to from bark.It is isolated with two from ginkgo leaf
The relevant another kind of compound of terpene ginkgolides structure, also referred to as Bilobalide.Therefore ginkgoterpene lactone by Ginkgolides a and B,
C, J, M and Bilobalide composition.
The most important purposes of ginkgo biloba crude extract is that it can control the decline of brain blood circulation disorder.Ginkgo leaf slightly carries
Efficiency of the object in terms of removing free radical is more taller than vitamin E, can prevent high-fat cell membrane from being aoxidized.And it is thin to cancer
Intracellular growth has significant inhibiting effect.
Invention content
The object of the present invention is to provide a kind of new applications of ginkgo biloba p.e.
The new application of ginkgo biloba p.e provided by the present invention is its application as fall webworms larva anti-feedant.
The ginkgo biloba p.e is following at least one：Ginkgolides, Bilobalide, ginkolide B, ginkalide A
And ginkgo biloba crude extract.
The ginkgolides is by Bilobalide, ginkalide A, ginkolide B and ginkalide C according to mass ratio 5:2:2:
1 composition.
The fall webworms larva anti-feedant made of above-mentioned ginkgo biloba p.e also belongs to protection scope of the present invention.
The study find that new application of the ginkgo biloba p.e in terms of pest control, provides a kind of efficient plant source
Anti-feedant, the harm for preventing fall webworms larva.Plant antifeedant is native compound, can be held very much in the natural environment
It changes places and is decomposed, less environmental pollution has higher environmental benefit.
Description of the drawings
Fig. 1 is to spray ginkgo biloba crude extract, the fall webworms larva quantity before and after ginkgolides and the schematic diagram for endangering shape,
Wherein, a, A are respectively fall webworms larva quantity before and after spraying ginkgolides and endanger the schematic diagram of shape；B, B is respectively to spray
It spills the fall webworms larva quantity before and after ginkgo biloba crude extract and endangers the schematic diagram of shape.
Fig. 2 is average scattering ratio of the different larval instar larva to different agents (ginkgo biloba crude extract, ginkgolides).
Fig. 3 a are that leaf disk is placed on graph paper to carry out areal calculation, and Fig. 3 b are that fall webworms larva is right in insect box
Leaf disk carries out the case where feeding.
Specific implementation mode
Below by specific embodiment, the present invention will be described, but the present invention is not limited thereto.
Experimental method used in following embodiments is conventional method unless otherwise specified；Institute in following embodiments
Reagent, biomaterial etc., are commercially available unless otherwise specified.
Ginkgo biloba crude extract used in following embodiments is the product of the general scientific instrument Co., Ltd of Town in Shanghai, wherein
Terpene lactones >=6%.
Experimental period is in July, 2015, and in Baodi District, Tianjin City, experimental subjects is to be endangered not on dry land willow for place selection
With age fall webworms larva, drug used is standard items, purity ＞ 99%.
Experimental method
10ml absolute alcohols are taken, respectively to 2g ginkgo biloba crude extracts, (group becomes 0.1g ginkgolides：Bilobalide：In ginkgo
Ester A：Ginkolide B：Ginkalide C=5:2:2:1) it is dissolved, then by above-mentioned ginkgo biloba crude extract and ginkgolides
Alcoholic solution is mixed with 70ml distilled water respectively, and finally acquired solution is put into the plastics watering can that volume is about 100ml and is used in combination
Control group is arranged in distilled water, spare after label.
The branch that selection is endangered by the fall webworms larva of different larval instar on dry land willow photographs to record it and takes at totally 108
Eat situation (see a, b in Fig. 1).Then worm leaf surface sprays 3 times respectively with 4 kinds of watering cans.Specific design scheme such as following table institute
Show：
1 different larval instar fall webworms larva of table handles branch number design scheme
Larva antifeedant is observed at interval after 5 hours, photographed to record (A, B in Fig. 1), and is counted according to front and back photo comparison
Calculate scattering ratio
Experimental result is shown in Fig. 2.
Analysis is carried out to result above and finds that 2 kinds of different agents compared with the control group have the feeding of fall webworms larva
Have apparent repellent effect, but individually take as seen from Figure 2 ginkgolides carry out experiment find its to larva repellent effect with
Ginkgo biloba crude extract is similar, but in view of extraction difficulty and economic factor, uses ginkgo biloba crude extract in actual application
It is more cost-effective.The increase with worm age is can be seen that by the variation tendency of curve, repellent effect first enhances and weakens afterwards,
Illustrate that pesticide supplying effect can be more obvious between 3 age of fall webworms larva.
Bilobalide individually is taken, ginkalide A, ginkolide B and ginkalide C are molten according to various concentration gradient respectively
In solution to 10ml alcohol, then mixed with 70ml distilled water, experimental design is as shown in the table.Using puncher by acquire come it is new
Fresh Bai Laye is fabricated to the identical leaf disk of diameter, is separately immersed in solution made above 10 seconds, then each round plastic
4 are put into insect box (100ml), for 3 age fall webworms larval feedings, is continued 24 hours (Fig. 3 b).Leaf disk is taken out, is put into
On 1 × 1mm graph papers (Fig. 3 a), the number of squares by counting graph paper calculates anti-food rate.
24 kinds of ginkgolides various concentration design schemes of table
The anti-food rate of 3 leaf disk of table fall webworms larval feeding after 3 kinds of various concentration ginkgolide monomers are handled
Bilobalide, ginkalide A and ginkolide B Antifeeding Effects are more preferably apparent it can be seen from data in table 3, put down
The sequence of equal anti-food rate is Bilobalide ＞ ginkolide B ＞ ginkalide A ＞ ginkalide Cs.Under 3 kinds of concentration, in gingko
Ester and ginkolide B show the anti-food rate of larva the gradient difference of various concentration, and the anti-food rate of ginkalide A does not occur
Apparent gradient difference, and ginkalide C does not show apparent Antifeeding Effects.The ginkolide B anti-food rate of high concentration
Highest is 36.6%.
Claims (1)
1. ginkgolides is as the application in fall webworms larva anti-feedant；The ginkgolides is：Bilobalide or ginkgo
Lactone B.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510863812.3A CN105454304B (en) | 2015-12-01 | 2015-12-01 | Ginkgolides is as the application in fall webworms larva anti-feedant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510863812.3A CN105454304B (en) | 2015-12-01 | 2015-12-01 | Ginkgolides is as the application in fall webworms larva anti-feedant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105454304A CN105454304A (en) | 2016-04-06 |
| CN105454304B true CN105454304B (en) | 2018-10-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510863812.3A Expired - Fee Related CN105454304B (en) | 2015-12-01 | 2015-12-01 | Ginkgolides is as the application in fall webworms larva anti-feedant |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107251910B (en) * | 2017-06-27 | 2020-04-07 | 北京林业大学 | Synergistic antifeedant for preventing and treating fall webworms and using method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1398524A (en) * | 2002-09-04 | 2003-02-26 | 浙江省农业科学院植物保护研究所 | Application of ginkgo as plant pesticide |
| CN103609597A (en) * | 2013-12-03 | 2014-03-05 | 福建农林大学 | Botanical synergistic compound pesticide |
-
2015
- 2015-12-01 CN CN201510863812.3A patent/CN105454304B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1398524A (en) * | 2002-09-04 | 2003-02-26 | 浙江省农业科学院植物保护研究所 | Application of ginkgo as plant pesticide |
| CN103609597A (en) * | 2013-12-03 | 2014-03-05 | 福建农林大学 | Botanical synergistic compound pesticide |
Non-Patent Citations (1)
| Title |
|---|
| "银杏叶粗提物对小菜蛾的拒避和生长发育抑制作用";徐红星 等，;《华东昆虫学报》;20020630;第11卷(第1期);第78-79页表1-3、图1 * |
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| CN105454304A (en) | 2016-04-06 |
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