CN105451707A

CN105451707A - External application composition for skin, including cell bio fluid replicating liquid

Info

Publication number: CN105451707A
Application number: CN201480043614.6A
Authority: CN
Inventors: 朴胜寒; 崔炤雄; 赵诚雅; 蔡秉根; 姜炳永; 韩相勋
Original assignee: Amorepacific Corp
Current assignee: Amorepacific Corp
Priority date: 2013-07-31
Filing date: 2014-07-31
Publication date: 2016-03-30
Also published as: KR102087136B1; CN110279607A; KR20150015090A; WO2015016637A1

Abstract

The present invention relates to an external application composition for skin, including a cell bio fluid replicating liquid and, more particularly, to an external application composition for skin, which increases the amount of moisture in skin and improves skin tone uniformity, skin texture, and skin transparency by including a cell bio fluid replicating liquid that includes minerals, monosaccharides and peptides.

Description

The Dermatologic preparation composition of fluid is imitated containing biofluid

Technical field

The present invention relates to the Dermatologic preparation composition imitating fluid containing biofluid, more specifically, the fluid of mimic biology liquid containing mineral, monosaccharide and peptide, thus the Dermatologic preparation composition of skin moisture content, the skin brightness uniformity, skin texture and skin transparency can be improved.

Background technology

In body, moisture is the composition accounting for maximum specific weight in our health, is about 50 ~ 70%, to be distributed in our health in all cells and body fluid.The half that people is losing body fat and protein can both sustain life, but in the body losing 10% during moisture health will be on the hazard, and may be dead when losing the moisture of more than 10%, therefore in body, moisture is the necessary composition that sustains life.

In this body, moisture not only plays conveying nutrient and refuse, prevents the sharply rising of body temperature and decline and maintains body temperature, the effect of constituting body inner tissue, also playing making tissue and joint become soft, the effect of protection important organ.In addition, the main component as blood forms the movable passageway into people's substance in vivo, plays the important function of medium in other various chemical reaction.

For the as above constancy maintaining in-vivo tissue, and become the movable passageway of substance in vivo and imitate fluid to skin as the biofluid of the medium of various chemical reaction and produce which kind of impact, also not carrying out research or report.

[prior art document]

Patent documentation 1: No. 2006-0015597th, KR published patent

Summary of the invention

Technical problem

The present inventor finds that biofluid is imitated fluid and demonstrated significantly excellent effect to improving skin condition, this completes the present invention.

Therefore, the object of the present invention is to provide a kind of biofluid by comprising the preparation containing mineral, monosaccharide, peptide etc. to imitate fluid, thus the Dermatologic preparation composition of skin condition can be improved.

Technical scheme

To achieve these goals, the invention provides a kind of containing comprise mineral, monosaccharide, peptide biofluid imitate the Dermatologic preparation composition of fluid as effective ingredient.

Invention effect

Compositions of the present invention contains the fluid prepared by mimic biology liquid, thus can provide the various skin condition improvement effects such as skin moisture content, skin brightness, the uniformity, skin texture and transparency raising.In addition, biofluid is imitated the composition such as mineral, monosaccharide, peptide contained in fluid and is played synergy by using simultaneously, thus demonstrates significant excellent effect.

Detailed description of the invention

The invention provides a kind of Dermatologic preparation composition imitating fluid containing biofluid.

The term " biofluid " used in the present invention is all liquid participating in metabolic activity while general reference forms the health of life entity, the viewpoint forming organism is called as " in body moisture (bodywater) ", or be expressed as with moving phase association of vital metabolic " water (bioavailablewater) that utilizes of life entity ", the mineral containing optimal proportion, concentration and electrolyte.Especially skin plays active the barrier action protecting human body to destroy from external environment condition by the protection of moisture and thermoregulation, and the biofluid be present in skin is known as and produces for recovering and water needed for the various activity improving skin condition.

Term " biofluid the imitates fluid " imitation used in the present invention is distributed in moisture in the body in body and prepares, the biofluid used in the present invention is imitated fluid and is contained the mineral, monosaccharide and the peptide that exist in biofluid, and alternatively containing lipid, have the composition similar to biofluid, therefore skin absorbing power is excellent.The constituent class that biofluid of the present invention imitates fluid is similar to the composition of the biofluid existed in skin, and the similar degree that refers in particular to mineral homeostasis is preferably 95%.

The mineral imitating fluid for biofluid of the present invention is the nucleus forming intracellular fluid, moves and intracellular chemistry reaction plays a role to the moisture between cell.Use more than a kind that is selected from calcium chloride, magnesium chloride, sodium chloride, potassium chloride, sodium phosphate and potassium phosphate in the present invention, more preferably comprise the mineral of more than 4 kinds.The content of mineral can be more than 0.00001 % by weight relative to composition total weight and less than 0.1 % by weight, is preferably 0.01 % by weight.When content is less than 0.00001 % by weight, ionic equilibrium cannot be formed; When content is more than 0.1 % by weight, destroys the balance with skin, on the contrary harmful effect is produced to skin.

Biofluid of the present invention is imitated the monosaccharide used in fluid and is provided the energy that can make cell activation, uses more than a kind that is selected from glucose, xylose, mannose, trehalose, fructose, galactose and lactose.The content of monosaccharide can be composition total weight more than 0.001 % by weight and less than 1 % by weight, be preferably 0.02 % by weight.When being not enough to 0.001 % by weight, sufficient activation capacity cannot be supplied; When more than 1 % by weight, cause use sense poor because of excessive saccharide, and sticky.

It is by the peptide of 2 ~ 19 Amino acid profiles that biofluid of the present invention imitates the peptide used in fluid, such as can use copper tripeptides, dipeptides, tetrapeptide, pentapeptide, six peptides, octapeptide, seven peptides, oligopeptide etc., but the increase molecular weight with length can become large, producing cost also can rise thus, permeability cell also reduces, and the peptide that therefore length is short is more useful.The aminoacid forming peptide can use more than a kind of being selected from alanine, aspartic acid, glutamic acid, glycine, histidine, lysine, agedoite, glutamine, arginine, tryptophan and tyrosine.Be developed the multiple peptide for cosmetics, wherein the representational example of most is copper tripeptides (GHK-Cu), palmityl Wushengtai (trade name Matrixyl), acetyl group six peptide (trade name Argireline) etc.Wherein, copper victory peptide is the material that the form be sequestered on Gly-His-Lys with copper is present in body, plays the effect of the somatomedin of Skin Cell.The content of peptide can be composition total weight more than 0.000001 % by weight and less than 0.1 % by weight, be preferably more than 0.00015 % by weight and below 0.01 weight.When being not enough to 0.000001 % by weight, be difficult to expect to obtain the effective skin improvement effects brought by peptide; When more than 0.1 % by weight, rise so high compared on effect unit price, cause the shortcoming in price, and other materials contained in compositions are combined, thus produce the bad phenomenon of stability.

In addition, biofluid of the present invention is imitated fluid and can be contained lipid further.Preferably, lecithin (lecithin) can be used in the present invention.Lecithin can form lipid film on skin, lecithin has hydrophilic radical and hydrophobic group and is similar to human-body biological film in molecule, elimination side effect can be provided, best moisturizing power and skin barrier restore funcitons and the barrier effect (occlusiveeffect) maintained needed for moisturizing power when being therefore applied to skin.The content of lecithin is more than 0.005 % by weight relative to composition total weight and less than 0.1 % by weight.When being not enough to 0.005 % by weight, sticky, absorption is felt and moisturizing sense improvement has little effect; When more than 0.1 % by weight, the stability in liquid-crystalization raw material can reduce, and when compositions of the present invention being used with soluble dosage forms, dispersion stabilization also can be deteriorated.

Compositions of the present invention can containing being more than 0.001 % by weight relative to the content of composition total weight and the biofluid of less than 99 % by weight imitates fluid, preferably containing more than 80 % by weight and less than 99 % by weight.When using with the amount being not enough to 0.001 % by weight, cannot fully demonstrate its effect; When using with the amount more than 99 % by weight, pH is too low and may produce skin irritation.

Compositions of the present invention is by supplementing not enough energy to skin, make the moisture inside and outside skin move smooth and easy and can provide and give the effect of skin vitality, and by and imitate with biofluid synergism that the composition that contains in fluid brings and more excellent effect can be provided.

In addition, Dermatologic preparation composition of the present invention is owing to having effect as above, therefore, it is possible to improve skin beauty water component, skin brightness, the uniformity, skin texture and transparency, thus can use as skin moisture-keeping compositions and skin-whitening compositions.

Compositions of the present invention can containing acceptable medium on cosmeceutical or Dermatology or base material by dosage form.It comprises all dosage forms of applicable local application, such as, can with solution, gel, solid, the anhydrous product of pasty state, in aqueous phase, disperse oil phase and obtain emulsion, suspension, microemulsion, microcapsule, subparticle ball or ion-type (liposome) and nonionic folliculus dispersant form, or frost, astringent, lotion, powder, ointment, spraying or the form that hides flaw rod provide.In addition, can also use with foam (foam) form, or use with the composite spray form of the propellant further containing compression.These compositionss can be obtained by the method that this area is conventional.

In addition, compositions of the present invention can also contain fatty material, organic solvent, lytic agent, concentrating agents, gel, softening agent, antioxidant, suspending agent, stabilization agent, foaming agent (foamingagent), aromatic, surfactant, water, ion-type or nonionic emulsifier, filler, chelating agen, chelating agent, preservative agent, vitamin, blocker, wetting agent, quintessence oil, dyestuff, pigment, hydrophilic or lipophile activating agent, the normally used auxiliary agent in cosmeceutical or Dermatology field of normally used other composition any etc. in lipid folliculus or cosmetics.Described auxiliary agent imports with amount normally used in cosmeceutical or Dermatology field.

In addition, compositions of the present invention, in order to increase skin improvement effects, can contain skin absorption enhancement material.

Below, by embodiment and dosage form example, formation of the present invention and effect are described in detail.But these embodiments and dosage form example provide to help to understand the present invention, and category of the present invention and scope are not limited to following embodiment.

[reference example 1] biofluid imitates the preparation of fluid (embodiment 1)

The potassium phosphate of the calcium chloride of the 0.0093mg as mineral composition, the magnesium chloride of 0.0093mg, the sodium chloride of 0.7435mg, the sodium phosphate of 0.2007mg, the potassium chloride of 0.0186mg and 0.0186mg is added in the Purified Water of 93.35g; And the trehalose, the copper victory peptide (manufacturer: Neurogenex, trade name: PEP102100mg (being equivalent to the copper victory peptide of 0.15mg)) that add as the glucose of the 10mg of monosaccharide, the xylose of 10mg, the mannose of 1mg and 0.1mg, then add the lecithin as lipid components of 0.4mg, and make it dissolve at 30 ~ 40 DEG C.

After being confirmed whether to dissolve completely, the impurity utilizing drainage screen to remove to produce in preparation process, thus prepared embodiment 1.

[reference example 2] biofluid imitates the preparation of fluid (embodiment 2)

Replace copper victory peptide and add mutually commensurability dipeptides (manufacturer: standing grain major company), in addition having prepared embodiment 2 by the method identical with above-mentioned reference example 1.

[reference example 3] biofluid imitates the preparation of fluid (embodiment 3)

Replace copper victory peptide and add mutually commensurability tetrapeptide (manufacturer: the star limited company (OrientStarsLLC) in east), in addition having prepared embodiment 3 by the method identical with above-mentioned reference example 1.

[reference example 4] biofluid imitates the preparation of fluid (embodiment 4)

Replace copper victory peptide and add mutually commensurability pentapeptide (manufacturer: DSM nutriment limited company (DSMNutritionalProductsLtd.)), in addition having prepared embodiment 4 by the method identical with above-mentioned reference example 1.

[reference example 5] biofluid imitates the preparation of fluid (embodiment 5)

Replace copper victory peptide and add mutually commensurability six peptides (manufacturer: monopolization Institute of Cell Biology (InstitutEuropeenDeBiologieCellulaire)), in addition having prepared embodiment 5 by the method identical with above-mentioned reference example 1.

[reference example 6] biofluid imitates the preparation of fluid (embodiment 6)

Replace copper victory peptide and add mutually commensurability octapeptide (manufacturer: An Xuan limited company (CaregenCo., Ltd.)), in addition having prepared embodiment 6 by the method identical with above-mentioned reference example 1.

[reference example 7] biofluid imitates the preparation of fluid (embodiment 7)

Replace copper victory peptide and add mutually commensurability oligopeptide (manufacturer: VevyEuropeSpA company), in addition having prepared embodiment 7 by the method identical with above-mentioned reference example 1.

[reference example 8] biofluid imitates the preparation of fluid (embodiment 8)

Mineral uses the magnesium chloride of 10mg a kind of, and monosaccharide uses the fructose of 20mg a kind of, has in addition prepared embodiment 8 by the method identical with above-mentioned reference example 1.

[reference example 9] biofluid imitates the preparation of fluid (embodiment 9)

Mineral uses the sodium chloride of 10mg a kind of, and monosaccharide uses the galactose of 20mg a kind of, has in addition prepared embodiment 9 by the method identical with above-mentioned reference example 1.

[reference example 10] biofluid imitates the preparation of fluid (embodiment 10)

Mineral uses the potassium chloride of 10mg a kind of, and monosaccharide uses the lactose of 20mg a kind of, has in addition prepared embodiment 10 by the method identical with above-mentioned reference example 1.

[reference example 11] biofluid imitates the preparation of fluid (embodiment 11)

Except not containing except lecithin, prepare embodiment 11 by the method identical with above-mentioned reference example 1.

[reference example 12] biofluid imitates the preparation of fluid (comparative example 1)

Only prepare comparative example 1 by purified water.

[reference example 13] biofluid imitates the preparation of fluid (comparative example 2)

In the Purified Water of 93.357g, add the trehalose of 0.1mg, copper victory peptide (manufacturer: Neurogenex, trade name: PEP102100mg (being equivalent to the copper victory peptide of 0.15mg)), and make it dissolve at 30 ~ 40 DEG C.

After being confirmed whether to dissolve completely, the impurity utilizing drainage screen to remove to produce in preparation process, thus prepared comparative example 2.

[reference example 14] biofluid imitates the preparation of fluid (comparative example 3)

Replace trehalose and add the glucose of 10mg, in addition having prepared comparative example 3 by the method identical with above-mentioned reference example 13.

[reference example 15] biofluid imitates the preparation of fluid (comparative example 4)

Replace trehalose and add the xylose of 10mg, in addition having prepared comparative example 4 by the method identical with above-mentioned reference example 13.

[reference example 16] biofluid imitates the preparation of fluid (comparative example 5)

Replace trehalose and add the mannose of 1mg, in addition having prepared comparative example 5 by the method identical with above-mentioned reference example 13.

[reference example 17] biofluid imitates the preparation of fluid (comparative example 6)

Replace trehalose and add the fructose of 0.1mg, in addition having prepared comparative example 6 by the method identical with above-mentioned reference example 13.

[reference example 18] biofluid imitates the preparation of fluid (comparative example 7)

Replace trehalose and add the galactose of 0.1mg, in addition having prepared comparative example 7 by the method identical with above-mentioned reference example 13.

[reference example 19] biofluid imitates the preparation of fluid (comparative example 8)

Replace trehalose and add the lactose of 0.1mg, in addition having prepared comparative example 8 by the method identical with above-mentioned reference example 13.

[reference example 20] biofluid imitates the preparation of fluid (comparative example 9)

In the Purified Water of 93.357g, add calcium chloride and the copper victory peptide (manufacturer: Neurogenex, trade name: PEP102100mg (being equivalent to the copper victory peptide of 0.15mg)) of 0.01mg, and make it dissolve at 30 ~ 40 DEG C.

After being confirmed whether to dissolve completely, the impurity utilizing drainage screen to remove to produce in preparation process, thus obtained comparative example 9.

[dosage form example 1 ~ 3 and compare dosage form example 1 ~ 3]

Utilize above-described embodiment 1 ~ 11 and comparative example 1 ~ 9, and prepared dosage form example 1 ~ 13 according to the composition of lower list 1 ~ 4 and compared dosage form example 1 ~ 10 (unit: % by weight).

Table 1

Table 2

Table 3

Table 4

Preparation method

1) at room temperature, by water-phase component (1 ~ 6) Homogeneous phase mixing.

2) ethanol phase constituent (7 ~ 10) be heated to 50 ~ 55 DEG C and make its uniform dissolution and mix.

3) when stirring, in above-mentioned (2), dropping into above-mentioned (1), and make its even solubilising.

[experimental example 1] skin moisture content improves effect

Utilize above-mentioned dosage form example 1 ~ 13 and compare dosage form example 1 ~ 10, the forearm 23 kinds of compositionss being coated on female subjects applying into the degree of coin-size, after 1 hour, measure moisture ( courage+Khazaka, GmbH & Co.KG, Leichlinge, Germany), and calculate the increase degree of skin beauty water component.Result of calculation represents in following table 5.

Table 5

Classification	The increase degree (%) of skin beauty water component
		Dosage form example 1	33％
Dosage form example 2	14％
		Dosage form example 3	10％
Dosage form example 4	13％
		Dosage form example 5	14％
Dosage form example 6	12％
		Dosage form example 7	13％
Dosage form example 8	13％
		Dosage form example 9	12％
Dosage form example 10	15％
		Dosage form example 11	15％
Dosage form example 12	14％
		Dosage form example 13	15％
Relatively dosage form example 1	5％
		Relatively dosage form example 2	6％
Relatively dosage form example 3	6％
		Relatively dosage form example 4	4％
Relatively dosage form example 5	5％
		Relatively dosage form example 6	4％
Relatively dosage form example 7	3％
		Relatively dosage form example 8	3％
Relatively dosage form example 9	4％
		Relatively dosage form example 10	3％

According to upper table 5, can confirm dosage form example 1 ~ 13 and compare compared with dosage form example 1 ~ 10, although imitate the increase degree difference to some extent of the content skin beauty water component of fluid according to biofluid, demonstrating excellent skin moisture content increases.In addition, can know that dosage form example 1 ~ 13 uses the biofluid of same composition to imitate fluid, but compositions intensive amount is different, dosage form example 1 is compared with dosage form example 2, on content many 2 times, but in skin moisture content increase, provide the significantly high effect exceeding more than 2 times.

By the above results, can know and imitate in fluid at biofluid, the effect of important factor is played in the combination of mineral composition, and secondly monosaccharide content is also important.

[experimental example 2] skin condition improves effect

In order to definitely confirm that the skin beauty water component using compositions of the present invention to bring improves, transparency improves, skin brightness improves, the uniformity improves and skin texture improves effect, above-mentioned dosage form example 1 is utilized to carry out following deepening experimentation.21 adult females (30.62 ± 3.77 years old) are as object, to before coating, coating after 20 minutes, 5 kinds of skins after 1 day, after 1 week state change measure (LUMISCAN, and the results are shown in following table 6 and table 7 TRUSYSTEM).

Table 6

Can confirm from the result of upper table 6, when using dosage form example 1, before coating, applying the water quantities improvement rate after 20 minutes is 40.65%, and transparency improves 8.21% in use after 1 week.Can also confirm that the skin brightness of coating after 20 minutes improves 0.57% compared to before coating, and skin brightness improves constantly from use.

Table 7

In addition, can know from the result of above-mentioned table 7, skin texture by second day, until constantly improve after 1 week, and has significant difference value from coating is after 20 minutes.

In addition, while carrying out above-mentioned test, using the questionnaire survey participated in test women and carry out for the project in following table 8 as object, thus have rated that skin beauty water component improves, transparency improves, skin brightness improves, the uniformity improves and skin texture improves effect.

Table 8

(using 8 points as standard, answering the % for " agreement " (being equivalent to 5 ~ 8 points))

From above-mentioned table 8, can confirm with experimental data similarly, high to the satisfaction of skin wet sense, high value is also demonstrated to the satisfaction of moisture sense.

Think that these results are the result confirming the effect that skin beauty water component increases, skin brightness improves, the uniformity improves, transparency improves and skin texture improves of imitating fluid as the biofluid of the object of the invention.

Claims

1. imitate fluid containing mineral, monosaccharide and peptide as the biofluid of effective ingredient.

2. biofluid according to claim 1 imitates fluid, it is characterized in that, described effective ingredient is further containing lipid.

3. biofluid according to claim 1 imitates fluid, it is characterized in that, described content of mineral substances is imitated total fluid weight relative to biofluid and is more than 0.00001 % by weight and less than 0.1 % by weight.

4. biofluid according to claim 1 imitates fluid, it is characterized in that, described monosaccharide content is imitated total fluid weight relative to biofluid and is more than 0.001 % by weight and less than 1 % by weight.

5. biofluid according to claim 1 imitates fluid, it is characterized in that, described peptide content is imitated total fluid weight relative to biofluid and is more than 0.000001 % by weight and less than 0.1 % by weight.

6. biofluid according to claim 2 imitates fluid, it is characterized in that, described lipid content is imitated total fluid weight relative to biofluid and is more than 0.005 % by weight and less than 0.1 % by weight.

7. biofluid according to claim 1 imitates fluid, it is characterized in that, described mineral is more than a kind of being selected from calcium chloride, magnesium chloride, sodium chloride, potassium chloride, sodium phosphate and potassium phosphate.

8. biofluid according to claim 1 imitates fluid, it is characterized in that, described monosaccharide is selected from more than a kind in glucose, xylose, mannose, trehalose, fructose, galactose and lactose.

9. biofluid according to claim 1 imitates fluid, it is characterized in that, described peptide is by 2 ~ 19 Amino acid profiles.

10. biofluid according to claim 9 imitates fluid, it is characterized in that, described aminoacid is selected from more than a kind in alanine, aspartic acid, glutamic acid, glycine, histidine, lysine, agedoite, glutamine, arginine, tryptophan and tyrosine.

11. biofluids according to claim 2 imitate fluid, it is characterized in that, described lipid is lecithin.

12. imitate the Dermatologic preparation composition of fluid containing the biofluid according to any one of claim 1 ~ 11.

13. will remove the Dermatologic preparation composition described in 12 according to right, it is characterized in that, described biofluid is imitated fluid content and is more than 0.001 % by weight relative to composition total weight and less than 99 % by weight.

14. Dermatologic preparation compositions according to claim 12 or 13, it is characterized in that, described compositions is skin moisture-keeping compositions.

15. Dermatologic preparation compositions according to claim 12 or 13, it is characterized in that, described compositions is skin-whitening compositions.

16. Dermatologic preparation compositions according to claim 12 or 13, it is characterized in that, described compositions is skin transparency improvement compositions.

17. Dermatologic preparation compositions according to claim 12 or 13, it is characterized in that, described compositions is skin brightness improvement compositions.

18. Dermatologic preparation compositions according to claim 12 or 13, it is characterized in that, described compositions is skin texture improvement compositions.

19. rights will go the biofluid according to any one of 1 ~ 11 to imitate the application of fluid in skin moisture-keeping.

20. rights will go the biofluid according to any one of 1 ~ 11 to imitate the application of fluid in skin-whitening.

21. rights will go the biofluid according to any one of 1 ~ 11 to imitate the application of fluid in the improvement of skin transparency.

22. rights will go the biofluid according to any one of 1 ~ 11 to imitate the application of fluid in skin brightness improvement.

23. rights will go the biofluid in 1 ~ 11 described in any one to imitate the application of fluid in skin texture improvement.