CN105441528A - Selective culture medium with high separation rate of staphylococcus saprophyticus - Google Patents
Selective culture medium with high separation rate of staphylococcus saprophyticus Download PDFInfo
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- CN105441528A CN105441528A CN201610027469.3A CN201610027469A CN105441528A CN 105441528 A CN105441528 A CN 105441528A CN 201610027469 A CN201610027469 A CN 201610027469A CN 105441528 A CN105441528 A CN 105441528A
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- selective medium
- staphylococcus saprophyticus
- separation rate
- citrate
- coetsoidin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/14—Streptococcus; Staphylococcus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/045—Culture media therefor
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Abstract
The invention relates to a selective culture medium with a high separation rate of staphylococcus saprophyticus. A formula for preparing 1000ml of the selective culture medium comprises 25g of soybean flour agar, 250ml of glucose liquid, 5g of sodium citrate, 0.5g of magnesium sulfate, 25g of calcium chloride, 9mg to 12mg of behenic acid, 0.9g of cattle heart leached powder, 0.2g of coetsoidin A and the balance of distilled water added to be 1000ml. A preparation method comprises the following steps: (1) taking lilium brownii and adding the water to be boiled for 1 hour; filtering and taking a filtrate; adjusting the volume until the concentration is 200g/L to obtain a lilium brownii boiled solution; (2) taking the soybean flour agar, the glucose liquid, the sodium citrate, the magnesium sulfate, the calcium chloride, the behenic acid, the cattle heart leached powder, the coetsoidin A, magnesium citrate and glycine and dissolving the materials into the lilium brownii boiled solution obtained by the step 1; uniformly mixing and filtering; adding the distilled water until the volume is constant and sterilizing for 2 times at a low temperature, wherein each time of sterilizing is lasted for 50min; after sterilizing, sub-packaging. Compared with the prior art, the selective culture medium has the characteristics of reliability in detection and high separation rate of the staphylococcus saprophyticus.
Description
Technical field
The present invention relates to field of medical examination, be specifically related to a kind of selective medium high to Staphylococcus saprophyticus separation rate.
Background technology
Staphylococcus is a group gram-positive cocci, different with chromogenesis according to biochemical reaction, can be divided into streptococcus aureus, staphylococcus epidermidis and Staphylococcus saprophyticus three kinds.Wherein streptococcus aureus mostly is pathogenic bacterium, and staphylococcus epidermidis causes a disease once in a while, and Staphylococcus saprophyticus is generally not pathogenic.Staphylococcus saprophyticus belongs to microorganism, and what pathogenic infection to everyone body showed is different.
Summary of the invention
Technical assignment of the present invention is for above the deficiencies in the prior art, provides a kind of selective medium high to Staphylococcus saprophyticus separation rate.
The technical scheme that the present invention solves its technical problem is:
A selective medium high to Staphylococcus saprophyticus separation rate, containing bean powder agar 25g in the formula of preparation 1000ml selective medium; Glucose Liquid 250ml; Trisodium Citrate 5g; Magnesium sulfate 0.5g; Calcium chloride 25g; Taro acid 9 ~ 12mg; OX-heart leaches powder 0.9g; Coetsoidin A 0.2g; Distilled water adds to 1000ml.
Further, every 1000ml selective medium is also containing glycine 5mg.
Further, every 1000ml selective medium is also containing citrate of magnesia 0.2g.
Further, the lily water cooking liquid 200 ~ 300ml of every 1000ml selective medium also containing concentration 200g/L.
Further, every 1000ml selective medium is also containing glycine 5mg; Citrate of magnesia 0.2g; The lily water cooking liquid 200 ~ 300ml of concentration 200g/L.
To a preparation method for the high selective medium of Staphylococcus saprophyticus separation rate, comprise the following steps:
(1) get lily and add water boil 1 hour, filter, get filtrate, adjustment volume is 200g/L to concentration, obtains lily water cooking liquid;
(2) bean powder agar is got; Glucose Liquid; Trisodium Citrate; Magnesium sulfate; Calcium chloride; Taro acid; OX-heart leaches powder; Coetsoidin A; Citrate of magnesia and glycine are dissolved in step 1 gained lily water cooking liquid, after mixing is filtered, and distilled water constant volume, temperature sterilization 2 times, each 50min, packing after sterilizing.
This beneficial effect of the invention is: the present invention compared with prior art, has and detects the feature reliable, Staphylococcus saprophyticus separation rate is high.
Embodiment
Below in conjunction with practical situation, the specific embodiment of the present invention is elaborated.
embodiment 1
Contain in the formula of preparation 1000ml selective medium: bean powder agar 25g; Glucose Liquid
250ml; Trisodium Citrate 5g; Magnesium sulfate 0.5g; Calcium chloride 25g; Taro acid 9mg; OX-heart leaches powder 0.9g; Coetsoidin A 0.2g; Distilled water adds to 1000ml.Preparation method: get bean powder agar; Glucose Liquid; Trisodium Citrate; Magnesium sulfate; Calcium chloride; Taro acid; OX-heart leaches powder; Coetsoidin A is dissolved in distilled water, after mixing is filtered, and distilled water constant volume, temperature sterilization 2 times, each 50min, packing after sterilizing.
embodiment 2
Contain in the formula of preparation 1000ml selective medium: bean powder agar 25g; Glucose Liquid 250ml; Trisodium Citrate 5g; Magnesium sulfate 0.5g; Calcium chloride 25g; Taro acid 12mg; OX-heart leaches powder 0.9g; Coetsoidin A 0.2g; Glycine 5mg; Distilled water adds to 1000ml.Preparation method: get bean powder agar; Glucose Liquid; Trisodium Citrate; Magnesium sulfate; Calcium chloride; Taro acid; OX-heart leaches powder; Coetsoidin A; Glycine is dissolved in distilled water, after mixing is filtered, and distilled water constant volume, temperature sterilization 2 times, each 50min, packing after sterilizing.
embodiment 3
Contain in the formula of preparation 1000ml selective medium: bean powder agar 25g; Glucose Liquid 250ml; Trisodium Citrate 5g; Magnesium sulfate 0.5g; Calcium chloride 25g; Taro acid 10mg; OX-heart leaches powder 0.9g; Coetsoidin A 0.2g; Citrate of magnesia 0.2g; Distilled water adds to 1000ml.Preparation method: get bean powder agar; Glucose Liquid; Trisodium Citrate; Magnesium sulfate; Calcium chloride; Taro acid; OX-heart leaches powder; Coetsoidin A; Citrate of magnesia is dissolved in distilled water, after mixing is filtered, and distilled water constant volume, temperature sterilization 2 times, each 50min, packing after sterilizing.
embodiment 4
Contain in the formula of preparation 1000ml selective medium: bean powder agar 25g; Glucose Liquid 250ml; Trisodium Citrate 5g; Magnesium sulfate 0.5g; Calcium chloride 25g; Taro acid 9mg; OX-heart leaches powder 0.9g; Coetsoidin A 0.2g; The lily water cooking liquid 200ml of concentration 200g/L; Distilled water adds to 1000ml.Preparation method: (1) is got lily and added water boil 1 hour, filters, gets filtrate, and adjustment volume is 200g/L to concentration, obtains lily water cooking liquid; (2) bean powder agar is got; Glucose Liquid; Trisodium Citrate; Magnesium sulfate; Calcium chloride; Taro acid; OX-heart leaches powder; Coetsoidin A; Be dissolved in step 1 gained lily water cooking liquid, after mixing is filtered, distilled water constant volume, temperature sterilization 2 times, each 50min, packing after sterilizing.
embodiment 5
Contain in the formula of preparation 1000ml selective medium: bean powder agar 25g; Glucose Liquid
250ml; Trisodium Citrate 5g; Magnesium sulfate 0.5g; Calcium chloride 25g; Taro acid 11mg; OX-heart leaches powder 0.9g; Coetsoidin A 0.2g; Glycine 5mg; Citrate of magnesia 0.2g; The lily water cooking liquid 300ml of concentration 200g/L; Distilled water adds to 1000ml.Preparation method is: (1) is got lily and added water boil 1 hour, filters, gets filtrate, and adjustment volume is 200g/L to concentration, obtains lily water cooking liquid; (2) bean powder agar is got; Glucose Liquid; Trisodium Citrate; Magnesium sulfate; Calcium chloride; Taro acid; OX-heart leaches powder; Coetsoidin A; Citrate of magnesia and glycine are dissolved in step 1 gained lily water cooking liquid, after mixing is filtered, and distilled water constant volume, temperature sterilization 2 times, each 50min, packing after sterilizing.
Gained Staphylococcus saprophyticus selective medium of the present invention has and detects the feature reliable, Staphylococcus saprophyticus separation rate is high, and be clinical data sufficient proof, pertinent data is as follows.
1 object and method:
1.1 selective medium preparations: establish 3 groups altogether, be respectively control group, embodiment 1 scheme group, embodiment 5 scheme group.Control group is staphylococcus selective chlorination calcium 110(CHAPMAN calcium chloride); Formula and the preparation method of embodiment 1 scheme group, embodiment 5 scheme group are shown in embodiment.
1.2 methods: 20 parts are determined via PCR be mixed into Staphylococcus saprophyticus in the sample of the Staphylococcus saprophyticus positive, be inoculated on substratum, hatch 24 hours at 37 DEG C.
1.3 statistical analysis: carry out statistical study with SPSS13.0, P<0.05 indicates significant.
2 results: in 20 parts of inoculation samples, microbial culture is separated and is accredited as Staphylococcus saprophyticus, and embodiment 1 scheme group is 12 examples, and embodiment 4 scheme group is 16 routine.Control group is 5 examples, and through statistical procedures, various embodiments of the present invention group compares with control group and has notable difference (P<0.001).Result shows, embodiment of the present invention Staphylococcus saprophyticus separation rate is apparently higher than existing selective medium.
The above is the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications are also considered as protection scope of the present invention.
Claims (6)
1. a selective medium high to Staphylococcus saprophyticus separation rate, is characterized in that: containing bean powder agar 25g in the formula of preparation 1000ml selective medium; Glucose Liquid 250ml; Trisodium Citrate 5g; Magnesium sulfate 0.5g; Calcium chloride 25g; Taro acid 9 ~ 12mg; OX-heart leaches powder 0.9g; Coetsoidin A 0.2g; Distilled water adds to 1000ml.
2. the selective medium high to Staphylococcus saprophyticus separation rate according to claim 1, is characterized in that: every 1000ml selective medium is also containing glycine 5mg.
3. the selective medium high to Staphylococcus saprophyticus separation rate according to claim 1, is characterized in that: every 1000ml selective medium is also containing citrate of magnesia 0.2g.
4. the selective medium high to Staphylococcus saprophyticus separation rate according to claim 1, is characterized in that: the lily water cooking liquid 200 ~ 300ml of every 1000ml selective medium also containing concentration 200g/L.
5. the selective medium high to Staphylococcus saprophyticus separation rate according to claim 1, is characterized in that: every 1000ml selective medium is also containing glycine 5mg; Citrate of magnesia 0.2g; The lily water cooking liquid 200 ~ 300ml of concentration 200g/L.
6., according to the selective medium high to Staphylococcus saprophyticus separation rate described in claim 1 to 5, it is characterized in that: the preparation method of described selective medium, comprises the following steps:
(1) get lily and add water boil 1 hour, filter, get filtrate, adjustment volume is 200g/L to concentration, obtains lily water cooking liquid;
(2) bean powder agar is got; Glucose Liquid; Trisodium Citrate; Magnesium sulfate; Calcium chloride; Taro acid; OX-heart leaches powder; Coetsoidin A; Citrate of magnesia and glycine are dissolved in step 1 gained lily water cooking liquid, after mixing is filtered, and distilled water constant volume, temperature sterilization 2 times, each 50min, packing after sterilizing.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105603043A (en) * | 2016-04-08 | 2016-05-25 | 董建红 | Staphylococcus aureus selective culture medium |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103409495A (en) * | 2013-01-13 | 2013-11-27 | 候素君 | Preparation method of saprophytic staphylococcus selective medium |
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- 2016-01-18 CN CN201610027469.3A patent/CN105441528A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103409495A (en) * | 2013-01-13 | 2013-11-27 | 候素君 | Preparation method of saprophytic staphylococcus selective medium |
Non-Patent Citations (2)
| Title |
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| 牛立新等: "三种百合鳞茎提取物的抑茵作用", 《广西植物》 * |
| 莫艳珠等: "Guidongnin抗炎、抑菌药理作用研究", 《贵州科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105603043A (en) * | 2016-04-08 | 2016-05-25 | 董建红 | Staphylococcus aureus selective culture medium |
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Application publication date: 20160330 |