CN105435248B - A kind of multi-functional bismuth selenide nano-complex, its preparation method and application - Google Patents
A kind of multi-functional bismuth selenide nano-complex, its preparation method and application Download PDFInfo
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Abstract
A kind of multi-functional bismuth selenide nano-complex, its preparation method and application, the present invention and biomedical sector, and in particular to a kind of multi-functional bismuth selenide nano-complex, its preparation method and application.The present invention is to solve existing thermochemotherapy nano material synthesis condition and process it is complicated, biological safety is poor, drugloading rate is low, lacks suitable imaging diagnostic function and light thermal property has much room for improvement, lack the problem of clinical trial is verified.A kind of particle diameter of multi-functional bismuth selenide nano-complex is 50nm~200nm, and the drugloading rate for loading doxorubicin hydrochloride is 3%~10%.Method：First, Bi is prepared2Se3Nanometer sheet；2nd, Bi is prepared2Se3@PDA nanoparticle dispersion liquids；3rd, multi-functional bismuth selenide nano-complex is prepared.The multi-functional bismuth selenide nano-complex of the present invention is used for the thermochemotherapy of malignant tumour as thermochemotherapy nano material or is used for the CT imaging diagnosis of malignant tumour as CT image-forming contrast mediums.
Description
Technical field
The present invention relates to biomedical sector, and in particular to a kind of multi-functional bismuth selenide nano-complex, its preparation method
And application.
Background technology
Photo-thermal therapy be grew up in recent years a kind of Noninvasive based on near-infrared laser, can Fast Fixed-point kill
Hinder the novel therapeutic technology of tumour, its principle is to utilize the nano material (photo-thermal therapy agent) with photo-thermal converting function by light
Can be converted into heat come to tumor tissues heat, when temperature reaches more than 42 degree, tumor tissues will occur Apoptosis or
Person's necrosis, and the normal structure of human body or organ will not then come to harm.However, in order to enable photo-thermal therapy is implemented more
Effectively and safely, there is an urgent need to diagnosing tumour position, size and profile before photo-thermal therapy is carried out by people, it is also necessary to treat
During determine photo-thermal therapy agent in the enrichment condition of lesions position and treatment is monitored in real time, and treatment after to curative effect
Assessed.Therefore, implement just to need to combine Tomographic Diagnosis Technology (such as CT imagings, MRI imagings and photoacoustic imaging during photo-thermal therapy
Deng) carry out guiding in real time and auxiliary.Further, since absorption and scattering process of the biological tissue to light, rely solely on photo-thermal therapy
It is extremely difficult to carry out thorough eradicating cancer cells.In order to overcome these problems, people are needed photo-thermal therapy and other therapies (such as
Chemotherapy, physics radiotherapy etc.) join together, more effectively thoroughly to treat tumour.Wherein, due to photo-thermal effect both
Tumour cell can be made more sensitive to chemotherapeutics and remarkably promote intake of the tumour cell to chemotherapeutics, by photo-thermal therapy
With chemotherapy join together (abbreviation thermochemotherapy) so that both therapies synergistic effect, under very low dosage,
To obtain significantly more efficient therapeutic effect, this is of great significance for reducing caused toxic side effect in therapeutic process.
In recent years, much there is nano material (such as gold nanorods, gold nanoshell, the Jenner absorbed more by force near infrared region
Rice cage and carbon nanotubes etc.) have been developed that as thermochemotherapy nano material and be widely used in the thermochemotherapy of tumour
In.Although these thermochemotherapy Application of micron have been achieved for good effect when tumor therapy experiments, specific
In application, they are also there are many limitations, such as synthesis condition and process are more complicated, biological safety is very poor, carry medicine
Measure low, the suitable imaging diagnostic function of shortage and light thermal property has much room for improvement, lack clinical trial verification etc..It is in addition, this kind of more
The species of function thermochemotherapy nano material or very limited amount of.Therefore, new multi-functional thermochemotherapy nano material is developed to use
It is necessary in the multi-modal diagnosis of tumour and thermochemotherapy.
Through retrieving related bismuth selenide (Bi both at home and abroad2Se3) document in terms of nano material and patent results show, in this hair
Before bright application, have no precedent Bi2Se3Nano material carries out carrying the report in terms of medicine is used for Thermochemotherapy of Tumor.
The content of the invention
The present invention is to solve existing thermochemotherapy nano material synthesis condition and process it is complicated, biological safety is poor, load
Dose is low, lacks the problem of suitable imaging diagnostic function and light thermal property have much room for improvement, lack clinical trial verification, and provides
A kind of multi-functional bismuth selenide nano-complex, its preparation method and application.
A kind of multi-functional bismuth selenide nano-complex, it is with organic high molecular polymer, dihydric alcohol, selenium source, bismuth salt, reduction
Agent, dopamine hydrochloride, Tri(Hydroxymethyl) Amino Methane Hydrochloride solution, doxorubicin hydrochloride and seralbumin aqueous solution are raw material
It is prepared, the particle diameter of the multi-functional bismuth selenide nano-complex is 50nm~200nm, and the multi-functional bismuth selenide nanometer is answered
The drugloading rate of doxorubicin hydrochloride is 3%~10% in compound.
A kind of preparation method of multi-functional bismuth selenide nano-complex follows the steps below：
First, organic high molecular polymer is dissolved completely in dihydric alcohol, obtains reaction system, added into reaction system
Selenium source and bismuth salt, after ultrasonic mixing is uniform, with the heating rate of 10 DEG C/min~20 DEG C/min by the temperature of reaction system from room
Temperature is warming up to 100 DEG C~250 DEG C, and reducing agent is then added into reaction system, under conditions of temperature is 100 DEG C~250 DEG C
5min~30min postcoolings are reacted to room temperature, are centrifuged and washed 3~5 times using the mixed liquor of acetone and water, collect sample, very
Sky is dry, obtains Bi2Se3Nanometer sheet；The volume ratio of water and acetone is 1 in the mixed liquor of the acetone and water:(1~5)；It is described
The quality of organic high molecular polymer and the volume ratio of dihydric alcohol are 1g:(100~200) mL；The quality and binary of the bismuth salt
The volume ratio of alcohol is 1g:(300~400) mL；The quality of the selenium source and the volume ratio of dihydric alcohol are 1g:(600~700) mL；
The volume ratio of the reducing agent and dihydric alcohol is 1mL:(60~70) mL；
2nd, dopamine hydrochloride is dissolved in Tri(Hydroxymethyl) Amino Methane Hydrochloride solution, after ultrasonic mixing is uniform, Xiang Qi
The middle Bi for adding step 1 and obtaining2Se3Nanometer sheet, after 3~9h of stirring at normal temperature, is centrifuged using deionized water and washs 3~5 times, so
After be scattered in dimethyl sulfoxide (DMSO), obtain Bi2Se3@PDA nanoparticle dispersion liquids；The Bi2Se3@PDA nanoparticle dispersion liquids
Concentration be (2~4) mg/mL；The quality of the dopamine hydrochloride and the volume of Tri(Hydroxymethyl) Amino Methane Hydrochloride solution
Than for 1g:(300~500) mL；The Bi2Se3The quality of nanometer sheet and the volume ratio of Tri(Hydroxymethyl) Amino Methane Hydrochloride solution
For 1g:(1500~2500) mL；The pH of the Tri(Hydroxymethyl) Amino Methane Hydrochloride solution is 8~10；
3rd, doxorubicin hydrochloride is dissolved in dimethyl sulfoxide (DMSO), after ultrasonic mixing is uniform, obtains doxorubicin hydrochloride dispersion liquid；
Doxorubicin hydrochloride dispersion liquid is added to the Bi that step 2 obtains2Se3In@PDA nanoparticle dispersion liquids, stirring at normal temperature 5~
15min, obtains mixed solution, is then added drop-wise in seralbumin aqueous solution mixed solution dropwise under ultrasound condition, adopts
Centrifuged and washed 3~5 times with deionized water, obtained solid, solid is scattered in phosphate buffer, be positioned over temperature as 4
DEG C refrigerator in preserve, obtain multi-functional bismuth selenide nano-complex；The concentration of the doxorubicin hydrochloride dispersion liquid is (5~20)
mg/mL；The doxorubicin hydrochloride dispersion liquid and Bi2Se3The volume ratio of@PDA nanoparticle dispersion liquids is 1:(1~4)；It is described mixed
The volume ratio for closing solution and seralbumin aqueous solution is 1:(2~6)；The quality of the solid and the volume of phosphate buffer
Than for 1g:(1000~2000) mL；The concentration of seralbumin aqueous solution described in step 3 is 5.0mg/mL.
A kind of application of multi-functional bismuth selenide nano-complex is using multi-functional bismuth selenide nano-complex as thermochemotherapy
Nano material is used for the thermochemotherapy of malignant tumour.
A kind of application of multi-functional bismuth selenide nano-complex is imaged multi-functional bismuth selenide nano-complex as CT
Contrast agent is used for the CT imaging diagnosis of malignant tumour.
The beneficial effects of the invention are as follows：
The preparation condition and process of multi-functional bismuth selenide nano-complex prepared by the present invention are simple；The multi-functional selenium prepared
The size for changing bismuth nano-complex can be by simply changing the matching of reaction raw materials, required temperature and reaction time
Freely to control；
Multi-functional bismuth selenide nano-complex prepared by the present invention successfully loads chemotherapeutic drugs Doxorubicin, has very high load
Dose (3%~10%), wherein drugloading rate can by control add reaction DOX amounts freely control, and have pH with closely it is red
The insoluble drug release property of outer photaesthesia, has significant chemotherapy effect to tumour cell；Nano-complex has near infrared region
It is relatively strong to absorb, there is excellent photo-thermal heating performance and photo and thermal stability；Nano-complex is under the irradiation of near-infrared laser, energy
It is enough that effectively killing tumor cell, thermochemotherapy significant effect are better than single photo-thermal therapy or chemotherapy effect；Nano-complex
Toxicity is low, has good biological safety；Nano-complex can significantly increase external, internal CT imaging signals.
Brief description of the drawings
Fig. 1 is the TEM figures for the multi-functional bismuth selenide nano-complex that one step 3 of embodiment obtains；
Fig. 2 is the grain size distribution for the multi-functional bismuth selenide nano-complex that one step 3 of embodiment obtains；
Fig. 3 is the Bi that one step 1 of embodiment obtains2Se3The multi-functional selenizing that nanometer sheet and one step 3 of embodiment obtain
The UV absorption contrast curve of bismuth nano-complex, wherein 1 is Bi2Se3Nanometer sheet, 2 be that multi-functional bismuth selenide is nano combined
Thing；
Fig. 4 is the Bi that one step 1 of embodiment obtains2Se3Nanometer sheet and one step 3 of various concentrations embodiment obtain more
The fluorescence spectrum comparison diagram of function bismuth selenide nano-complex dispersion liquid, wherein 1 is Bi2Se3Nanometer sheet, 2 be that concentration is 4 μ g/mL
Multi-functional bismuth selenide nano-complex dispersion liquid, 3 be concentration be 8 μ g/mL multi-functional bismuth selenide nano-complex dispersion liquid,
4 be the multi-functional bismuth selenide nano-complex dispersion liquid that concentration is 16 μ g/mL；
Fig. 5 is the Bi that one step 1 of embodiment obtains2Se3The Bi that nanometer sheet, one step 2 of embodiment obtain2Se3@PDA receive
Bi in rice corpuscles dispersion liquid2Se3The multi-functional bismuth selenide nano-complex that@PDA nano-particles and one step 3 of embodiment obtain
FTIR spectrum figure, wherein 1 is Bi2Se3Nanometer sheet, 2 be Bi2Se3Bi in@PDA nanoparticle dispersion liquids2Se3@PDA receive
Rice corpuscles, 3 be multi-functional bismuth selenide nano-complex；
Fig. 6 is the drug release patterns of pH sensitivities, wherein 1 is drug release patterns of the pure DOX under the conditions of pH=7.4,2
It is drug release patterns of the multi-functional bismuth selenide nano-complex under the conditions of pH=7.4,3 is that multi-functional bismuth selenide is nano combined
Thing is medicine of the multi-functional bismuth selenide nano-complex under the conditions of pH=4.5 in the drug release patterns under the conditions of pH=5.5,4
Thing release profiles；
Fig. 7 is the medicine for the multi-functional bismuth selenide nano-complex dispersion liquid near infrared light sensitivity that the PBS of different pH disperses
Correlation curve is discharged, wherein 1 is the multi-functional bismuth selenide nano-complex dispersion liquids that disperse of PBS of pH=4.5,2 be pH=5.5
The multi-functional bismuth selenide nano-complex dispersion liquids that disperse of PBS, 3 be the multi-functional bismuth selenide nanometers disperseed of PBS of pH=7.4
Compound dispersion liquid, black arrow meaning is laser irradiation time section；
Fig. 8 is the multi-functional bismuth selenide nano-complex dispersion liquid of various concentrations and HeLa cells be incubated jointly it is 24 and 48 small
When after cell viability figure, wherein 1 when being 24 small, 2 when being 48 small；
Fig. 9 is the photo-thermal heating curve of the multi-functional bismuth selenide nano-complex dispersion liquid of various concentrations, wherein 1 is 0 μ g/
ML, 2 be 5 μ g/mL, and 3 be 20 μ g/mL, and 4 be 50 μ g/mL, and 5 be 100 μ g/mL, and 6 be 200 μ g/mL；
Figure 10 is the photo-thermal heating temperature difference figure of the multi-functional bismuth selenide nano-complex dispersion liquid of various concentrations；
Figure 11 is the photo-thermal ramp cycle figure of multi-functional bismuth selenide nano-complex dispersion liquid, wherein 1 is that laser is opened, 2
It is laser shutdown；
Figure 12 is multi-functional bismuth selenide nano-complex, does not carry the Bi of medicine2Se3Nano-complex (Bi2Se3@PDA/HSA)
With pure DOX to cell viability comparison diagram after HeLa cell thermochemotherapies, wherein 1 is multi-functional bismuth selenide nano-complex, 2 be not carry
The Bi of medicine2Se3Nano-complex (Bi2Se3@PDA/HSA), 3 be pure DOX；
Figure 13 is the external CT images of multi-functional bismuth selenide nano-complex, wherein 1 is that Bi concentration of element is 0mmol/
L, 2 be that Bi concentration of element is 4.56mmol/L, 3 is that Bi concentration of element is 13.7mmol/L, 4 is that Bi concentration of element is
27.4mmol/L, 5 are that Bi concentration of element is 36.5mmol/L, 6 is that Bi concentration of element is 45.6mmol/L；
Figure 14 is external CT imaging signals (CT values, HU) the enhancing block diagram of multi-functional bismuth selenide nano-complex；
Mouse CT images before Figure 15 is the multi-functional bismuth selenide nano-complex of injection；
Figure 16 is mouse CT images after the multi-functional bismuth selenide nano-complex of injection, wherein 1 is tumour.
Embodiment
Embodiment one：A kind of multi-functional bismuth selenide nano-complex of present embodiment, it is gathered with organic polymer
Compound, dihydric alcohol, selenium source, bismuth salt, reducing agent, dopamine hydrochloride, Tri(Hydroxymethyl) Amino Methane Hydrochloride solution, hydrochloric acid Ah mould
Element and seralbumin aqueous solution are prepared for raw material, the particle diameter of the multi-functional bismuth selenide nano-complex for 50nm~
200nm, the drugloading rate of doxorubicin hydrochloride is 3%~10% in the multi-functional bismuth selenide nano-complex.
Embodiment two：The present embodiment is different from the first embodiment in that：The organic polymer polymerization
Thing is the mixture of one or both of polyvinylpyrrolidone and polyacrylic acid；The dihydric alcohol is ethylene glycol, propane diols
With one kind in butanediol or wherein several mixtures；The selenium source is in sodium selenite, potassium selenite and selenium dioxide
A kind of or wherein several mixture；The bismuth salt is one kind or wherein several mixed in bismuth nitrate, bismuth chloride and bismuth acetate
Compound；The reducing agent is a kind of or wherein several in azanol, morpholine borine, sodium borohydride, glucose and ascorbic acid mixes
Compound；The pH of the Tri(Hydroxymethyl) Amino Methane Hydrochloride solution is 8.0~10；The seralbumin aqueous solution behaviour serum
The mixture of one or both of albumin aqueous solution and Bovine Serum Albumin in Aqueous Solution, the seralbumin aqueous solution
Concentration is 5.0mg/mL.Other are identical with embodiment one.
Embodiment three：A kind of preparation method of multi-functional bismuth selenide nano-complex of present embodiment be according to
What lower step carried out：
First, organic high molecular polymer is dissolved completely in dihydric alcohol, obtains reaction system, added into reaction system
Selenium source and bismuth salt, after ultrasonic mixing is uniform, with the heating rate of 10 DEG C/min~20 DEG C/min by the temperature of reaction system from room
Temperature is warming up to 100 DEG C~250 DEG C, and reducing agent is then added into reaction system, under conditions of temperature is 100 DEG C~250 DEG C
5min~30min postcoolings are reacted to room temperature, are centrifuged and washed 3~5 times using the mixed liquor of acetone and water, collect sample, very
Sky is dry, obtains Bi2Se3Nanometer sheet；The volume ratio of water and acetone is 1 in the mixed liquor of the acetone and water:(1~5)；It is described
The quality of organic high molecular polymer and the volume ratio of dihydric alcohol are 1g:(100~200) mL；The quality and binary of the bismuth salt
The volume ratio of alcohol is 1g:(300~400) mL；The quality of the selenium source and the volume ratio of dihydric alcohol are 1g:(600~700) mL；
The volume ratio of the reducing agent and dihydric alcohol is 1mL:(60~70) mL；
2nd, dopamine hydrochloride is dissolved in Tri(Hydroxymethyl) Amino Methane Hydrochloride solution, after ultrasonic mixing is uniform, Xiang Qi
The middle Bi for adding step 1 and obtaining2Se3Nanometer sheet, after 3~9h of stirring at normal temperature, is centrifuged using deionized water and washs 3~5 times, so
After be scattered in dimethyl sulfoxide (DMSO), obtain Bi2Se3@PDA nanoparticle dispersion liquids；The Bi2Se3@PDA nanoparticle dispersion liquids
Concentration be (2~4) mg/mL；The quality of the dopamine hydrochloride and the volume of Tri(Hydroxymethyl) Amino Methane Hydrochloride solution
Than for 1g:(300~500) mL；The Bi2Se3The quality of nanometer sheet and the volume ratio of Tri(Hydroxymethyl) Amino Methane Hydrochloride solution
For 1g:(1500~2500) mL；The pH of the Tri(Hydroxymethyl) Amino Methane Hydrochloride solution is 8~10；
3rd, doxorubicin hydrochloride is dissolved in dimethyl sulfoxide (DMSO), after ultrasonic mixing is uniform, obtains doxorubicin hydrochloride dispersion liquid；
Doxorubicin hydrochloride dispersion liquid is added to the Bi that step 2 obtains2Se3In@PDA nanoparticle dispersion liquids, stirring at normal temperature 5~
15min, obtains mixed solution, is then added drop-wise in seralbumin aqueous solution mixed solution dropwise under ultrasound condition, adopts
Centrifuged and washed 3~5 times with deionized water, obtained solid, solid is scattered in phosphate buffer, be positioned over temperature as 4
DEG C refrigerator in preserve, obtain multi-functional bismuth selenide nano-complex；The concentration of the doxorubicin hydrochloride dispersion liquid is (5~20)
mg/mL；The doxorubicin hydrochloride dispersion liquid and Bi2Se3The volume ratio of@PDA nanoparticle dispersion liquids is 1:(1~4)；It is described mixed
The volume ratio for closing solution and seralbumin aqueous solution is 1:(2~6)；The quality of the solid and the volume of phosphate buffer
Than for 1g:(1000~2000) mL；The concentration of seralbumin aqueous solution described in step 3 is 5.0mg/mL.Specific embodiment party
Formula four：Present embodiment is unlike embodiment three：Organic high molecular polymer described in step 1 is polyethylene
The mixture of one or both of pyrrolidones and polyacrylic acid.Other are identical with embodiment three.
Embodiment five：Present embodiment is unlike embodiment three or four：Two described in step 1
First alcohol is one kind or wherein several mixtures in ethylene glycol, propane diols and butanediol.Other with embodiment three or
Four is identical.
Embodiment six：Unlike one of present embodiment and embodiment three to five：Institute in step 1
Selenium source is stated as one kind in sodium selenite, potassium selenite and selenium dioxide or wherein several mixtures；Bismuth described in step 1
Salt is one kind or wherein several mixtures in bismuth nitrate, bismuth chloride and bismuth acetate.Other and embodiment three to five
One of it is identical.
Embodiment seven：Unlike one of present embodiment and embodiment three to six：Institute in step 1
Reducing agent is stated as mixture a kind of or wherein several in azanol, morpholine borine, sodium borohydride, glucose and ascorbic acid.Its
He is identical with one of embodiment three to six.
Embodiment eight：Unlike one of present embodiment and embodiment three to seven：Institute in step 3
State the mixed of one or both of seralbumin aqueous solution behaviour seralbumin aqueous solution and Bovine Serum Albumin in Aqueous Solution
Compound.Other are identical with one of embodiment three to seven.
Embodiment nine：A kind of application of multi-functional bismuth selenide nano-complex of present embodiment is by multi-functional selenium
Change the thermochemotherapy that bismuth nano-complex is used for malignant tumour as thermochemotherapy nano material.
Embodiment ten：A kind of application of multi-functional bismuth selenide nano-complex of present embodiment is by multi-functional selenium
Change the CT imaging diagnosis that bismuth nano-complex is used for malignant tumour as CT image-forming contrast mediums.
Beneficial effects of the present invention are verified using following embodiments：
Embodiment one：A kind of preparation method of multi-functional bismuth selenide nano-complex follows the steps below：
First, 0.25g PVP are dissolved completely in 40.0mL dihydric alcohols, obtain reaction system, added into reaction system
60mgNa2SeO3With 112.5mg Bi (NO3)3·5H2O, after ultrasonic mixing is uniform, with the heating rate of 15 DEG C/min by reactant
Then the temperature of system adds 0.6mL hydroxylamine solutions from room temperature to 180 DEG C into reaction system, in the bar that temperature is 180 DEG C
10min postcoolings are reacted under part to room temperature, is centrifuged and washed 3~5 times using the mixed liquor of acetone and water, collect sample, vacuum
It is dry, obtain Bi2Se3Nanometer sheet；The volume ratio of water and acetone is 1 in the mixed liquor of the acetone and water:5；
2nd, by 24.76mg dopamine hydrochlorides be dissolved in 10.0mL pH be 8.5 Tri(Hydroxymethyl) Amino Methane Hydrochloride
Solution, after ultrasonic mixing is uniform, adds the Bi that 5mL step 1 obtains thereto2Se3Nanometer sheet, after stirring at normal temperature 6h, using going
Ionized water is centrifuged and washed 3~5 times, is then dispersed in dimethyl sulfoxide (DMSO), is obtained Bi2Se3@PDA nanoparticle dispersion liquids；
3rd, 10mg doxorubicin hydrochlorides are dissolved in 1mL dimethyl sulfoxide (DMSO)s, after ultrasonic mixing is uniform, obtain doxorubicin hydrochloride
Dispersion liquid；Doxorubicin hydrochloride dispersion liquid is added to the Bi that step 2 obtains2Se3In@PDA nanoparticle dispersion liquids, stirring at normal temperature
10min, obtains mixed solution, and then concentration that mixed solution is added drop-wise to 12.0mL dropwise under ultrasound condition is 5.0mg/mL
Seralbumin aqueous solution in, centrifuged and washed 3~5 times using deionized water, obtained solid, solid is scattered in phosphate
In buffer solution, it is positioned in the refrigerator that temperature is 4 DEG C and preserves, obtain multi-functional bismuth selenide nano-complex.
Fig. 1 is the TEM figures for the multi-functional bismuth selenide nano-complex that one step 3 of embodiment obtains；Fig. 2 is embodiment one
The grain size distribution for the multi-functional bismuth selenide nano-complex that step 3 obtains；Fig. 3 is the Bi that one step 1 of embodiment obtains2Se3
The UV absorption contrast curve for the multi-functional bismuth selenide nano-complex that nanometer sheet and one step 3 of embodiment obtain, wherein 1
It is Bi2Se3Nanometer sheet, 2 be multi-functional bismuth selenide nano-complex；Fig. 4 is the Bi that one step 1 of embodiment obtains2Se3Nanometer sheet
The fluorescence spectrum comparison diagram of the multi-functional bismuth selenide nano-complex dispersion liquid obtained with one step 3 of various concentrations embodiment, its
In 1 be Bi2Se3Nanometer sheet, 2 be the multi-functional bismuth selenide nano-complex dispersion liquid that concentration is 4 μ g/mL, and 3 be that concentration is 8 μ g/
The multi-functional bismuth selenide nano-complex dispersion liquid of mL, 4 be that the multi-functional bismuth selenide nano-complex that concentration is 16 μ g/mL disperses
Liquid；Fig. 5 is the Bi that one step 1 of embodiment obtains2Se3The Bi that nanometer sheet, one step 2 of embodiment obtain2Se3@PDA nanoparticles
Bi in sub- dispersion liquid2Se3In Fu for the multi-functional bismuth selenide nano-complex that@PDA nano-particles and one step 3 of embodiment obtain
Leaf infrared spectrogram, wherein 1 is Bi2Se3Nanometer sheet, 2 be Bi2Se3Bi in@PDA nanoparticle dispersion liquids2Se3@PDA nanoparticles
Son, 3 be multi-functional bismuth selenide nano-complex.The result shows that irregular shape is presented in multi-functional bismuth selenide nano-complex, put down
Equal particle diameter 112nm, particle diameter distribution are homogeneous；There is relatively strong absorption near infrared region, absorption value is higher than Bi2Se3Nanometer sheet；Fluorescence light
Spectrum and FTIR spectrum show that DOX is successfully loaded into multi-functional bismuth selenide nano-complex.
Embodiment two：The vitro drug release experiment of multi-functional bismuth selenide nano-complex：
Drugloading rate is tested and calculated：The multi-functional bismuth selenide nano-complex dispersion liquids of the 1.0mg/mL of 100 μ L are added to
In 3.0mL dilute hydrochloric acid solutions (pH=5), it is placed in darkroom and is stirred overnight, its fluorescence spectrum is tested using sepectrophotofluorometer,
Drugloading rate is calculated using DOX fluorescence standard curves.Result of calculation shows that the drugloading rate of multi-functional bismuth selenide nano-complex is
3.25%.
The drug release experiment of pH sensitivities：The multi-functional selenizing that the PBS of 1.0mL differences pH (4.5,5.5,7.4) is disperseed
Bismuth nano-complex dispersion liquid is fitted into dialysis tubing, is immersed in respectively in the PBS dialysis liquid that 80mL corresponds to pH, is placed in 37 DEG C of darkrooms
Middle gentle agitation, at selected time point, 2.0mL solution is taken out from system, it is tested using sepectrophotofluorometer respectively
Fluorescence spectrum, calculates drug accumulation burst size using DOX fluorescence standard curves, draws drug release patterns.Fig. 6 is pH sensitivities
Drug release patterns, wherein 1 is drug release patterns of the pure DOX under the conditions of pH=7.4,2 be that multi-functional bismuth selenide nanometer is answered
Compound in the drug release patterns under the conditions of pH=7.4,3 is multi-functional bismuth selenide nano-complex under the conditions of pH=5.5
Drug release patterns, 4 are drug release patterns of the multi-functional bismuth selenide nano-complex under the conditions of pH=4.5.Can from figure
To find out, compared with pure DOX, multi-functional bismuth selenide nano-complex can realize medicament slow release, and with the reduction of pH, medicine
Rate of release increase, illustrates that multi-functional bismuth selenide nano-complex has the insoluble drug release property of pH sensitivities.
The drug release experiment of near infrared light sensitivity：More work(that the PBS of 2.0mL differences pH (4.5,5.5,7.4) is disperseed
Energy bismuth selenide nano-complex dispersion liquid irradiates 8min (laser unlatching) under 808nm laser, is placed in dark and is cultivated at 37 DEG C
1h (laser shutdown), so circulation.The medicine fluorescence spectrum discharged in supernatant is measured at selected time point, after Centrifuge A sample,
Drug accumulation burst size is calculated using DOX fluorescence standard curves, draws drug release patterns.The PBS that Fig. 7 is different pH disperses
The insoluble drug release correlation curve of multi-functional bismuth selenide nano-complex dispersion liquid near infrared light sensitivity, wherein 1 is pH=4.5
The multi-functional bismuth selenide nano-complex dispersion liquid that PBS disperses, 2 be that the multi-functional bismuth selenide nanometers disperseed of PBS of pH=5.5 are answered
Compound dispersion liquid, 3 be the multi-functional bismuth selenide nano-complex dispersion liquids that disperse of PBS of pH=7.4, and black arrow meaning is sharp
Light irradiates the period.Experimental result, when opening laser irradiation, the drug release rate of multi-functional bismuth selenide nano-complex is shown
Increase is write, and is closed after laser, rate of release reduces, and pH is lower, and this phenomenon is more obvious, this illustrates multi-functional selenizing
Bismuth nano-complex has the insoluble drug release property of near infrared light sensitivity.
Embodiment three：Multi-functional bismuth selenide nano-complex is to HeLa cell chemotherapy effect test experiments：
HeLa cells are placed in overnight incubation on 96 well culture plates, the multi-functional bismuth selenide for adding various concentrations is nano combined
Thing dispersion liquid (0,2,5,10,20,50,100,150,200 μ g/mL) continue respectively culture 24 and 48 it is small when after, tried with CCK-8
Agent box measures cell viability.Fig. 8 is that the multi-functional bismuth selenide nano-complex dispersion liquid of various concentrations is incubated jointly with HeLa cells
Educate 24 and 48 it is small when after cell viability figure, wherein 1 when being 24 small, 2 when being 48 small.It can be seen from the figure that with multi-functional
The increase of bismuth selenide nano-complex dispersion liquid concentration and incubation time, its fragmentation effect to HeLa cells is better, illustrates more
Function bismuth selenide nano-complex is to the significant chemotherapy effect of tumour cell.
Example IV：Multi-functional bismuth selenide nano-complex light thermal property test：
Photo-thermal temperature rise effect is tested：The multi-functional bismuth selenide nano-complex of various concentrations that cumulative volume is 3.0mL is disperseed
Liquid (0,5,20,50,100, the 200 μ g/mL) laser of 808nm irradiates 10 minutes, is during which measured using thermocouple every 10 seconds
System temperature.Fig. 9 is the photo-thermal heating curve of the multi-functional bismuth selenide nano-complex dispersion liquid of various concentrations, wherein 1 is 0 μ
G/mL, 2 be 5 μ g/mL, and 3 be 20 μ g/mL, and 4 be 50 μ g/mL, and 5 be 100 μ g/mL, and 6 be 200 μ g/mL；Figure 10 is various concentrations
The photo-thermal heating temperature difference figure of multi-functional bismuth selenide nano-complex dispersion liquid.It can be seen from the figure that multi-functional bismuth selenide nanometer
Compound dispersion liquid can be rapidly heated under the irradiation of 808nm lasers, and as the increase of concentration, its temperature rise effect are notable
Increase, this illustrates that multi-functional bismuth selenide nano-complex has excellent photo-thermal heating performance.
Photo and thermal stability is tested：The multi-functional bismuth selenide nano-complex dispersion liquid of the 100 μ g/mL of 3.0mL is utilized
808nm laser irradiates 10 minutes, then naturally cools to room temperature, is again turned on laser illumination afterwards 10 minutes, natural cooling,
So circulation 5 times, thermocouple record temperature change.Figure 11 is that the photo-thermal heating of multi-functional bismuth selenide nano-complex dispersion liquid follows
Ring figure, wherein 1 is that laser is opened, 2 be laser shutdown.The result shows that multi-functional bismuth selenide nano-complex is swashed using 808nm
Its photo-thermal heating performance remains unchanged after light device irradiates repeatedly, this illustrates that multi-functional bismuth selenide nano-complex has excellent light
Heat endurance.
Embodiment five：Multi-functional bismuth selenide nano-complex tests HeLa cell thermochemotherapies measure of merit：
HeLa cells are placed in overnight incubation on 96 well culture plates, the DOX of various concentrations is added, does not carry the Bi of medicine2Se3Receive
Rice compound (Bi2Se3@PDA/HSA), multi-functional bismuth selenide nano-complex dispersion liquid (0,5,10,20,50,100 μ g/mL) incubates
Educate culture 12 it is small when after, discard old culture medium, wash 3 times, add fresh culture, using 808nm laser illumination 5min,
Cell continues after cultivating 24h, with CCK-8 kit measurement cell viabilities.Figure 12 is multi-functional bismuth selenide nano-complex, not
Carry the Bi of medicine2Se3Nano-complex (Bi2Se3@PDA/HSA) and pure DOX to cell viability comparison diagram after HeLa cell thermochemotherapies,
Wherein 1 is multi-functional bismuth selenide nano-complex, and 2 be the Bi for not carrying medicine2Se3Nano-complex (Bi2Se3@PDA/HSA), 3 are
Pure DOX.It can be seen from the figure that under the irradiation of near-infrared laser, multi-functional bismuth selenide nano-complex is to HeLa cells
Fragmentation effect is apparently higher than the Bi for not carrying medicine2Se3Nano-complex (Bi2Se3@PDA/HSA) or pure DOX, illustrate multi-functional selenium
Change bismuth nano-complex and single photo-thermal therapy or chemotherapy effect are better than to the thermochemotherapy significant effect of tumour cell.
Embodiment six：Multi-functional bismuth selenide nano-complex is external, the experiment of internal CT imaging tests：
External CT imaging tests：By multi-functional bismuth selenide nano-complex (the Bi concentration of element of various concentrations：0、4.56、
13.7th, 27.4,36.5,45.6mmol/L) it is placed in 1.5mL centrifuge tubes, it is scanned CT letters using toy CT Image-forming instruments
Number, carry out graphical analysis and the calculating of CT values using supporting CT softwares.Figure 13 is the body of multi-functional bismuth selenide nano-complex
Outer CT images, wherein 1 is that Bi concentration of element is 0mmol/L, 2 is that Bi concentration of element is 4.56mmol/L, 3 is Bi concentration of element
It is that Bi concentration of element is 27.4mmol/L, 5 is that Bi concentration of element is 36.5mmol/L, 6 is that Bi elements are dense for 13.7mmol/L, 4
Spend for 45.6mmol/L；Figure 14 is external CT imaging signals (CT values, HU) the enhancing column of multi-functional bismuth selenide nano-complex
Figure.The result shows that multi-functional bismuth selenide nano-complex can significantly increase system CT signal strengths, and with solution concentration
Increase, CT values (HU) increase, wherein CT values are positively correlated with system concentration.It is computed, multi-functional bismuth selenide nano-complex is to X
Gamma ray absorption coefficient is 7.53HU mmol Bi/L, hence it is evident that higher than the absorption coefficient of clinical common CT contrast agent Iohexol
(3.93HU mmol I/L), illustrates that the CT imaging effects of multi-functional bismuth selenide nano-complex are better than than Iohexol.
Internal CT imaging tests：The 100 multi-functional bismuth selenides of μ L of nude mice intratumor injection for being built with tumor model are nano combined
The PBS dispersion liquids (5.0mg/mL) of thing, mouse is placed on isoflurane anesthesia to carry out CT in toy CT imaging system instruments and sweeps
Retouch, collect data and carry out image reconstruction afterwards.Mouse CT images before Figure 15 is the multi-functional bismuth selenide nano-complex of injection,
Figure 16 is mouse CT images after the multi-functional bismuth selenide nano-complex of injection, wherein 1 is tumour.The result shows that before injection
Mouse tumor region does not find CT signals, and after multi-functional bismuth selenide nano-complex dispersion liquid is injected, mouse tumor area
Domain shows bright CT signals, and the position of tumour and size are all high-visible, illustrates multi-functional bismuth selenide nano-complex energy
Enough significantly increase in vivo CT imaging signals.
Claims (6)
1. a kind of preparation method of multi-functional bismuth selenide nano-complex, it is characterised in that multi-functional bismuth selenide nano-complex
Preparation method follows the steps below：
First, organic high molecular polymer is dissolved completely in dihydric alcohol, obtains reaction system, selenium source is added into reaction system
And bismuth salt, after ultrasonic mixing is uniform, with the heating rate of 10 DEG C/min~20 DEG C/min by the temperature of reaction system from room temperature liter
Then temperature adds reducing agent to 100 DEG C~250 DEG C into reaction system, reacted under conditions of being 100 DEG C~250 DEG C in temperature
5min~30min postcoolings are centrifuged using the mixed liquor of acetone and water and washed 3~5 times, collect sample, vacuum is done to room temperature
It is dry, obtain Bi2Se3Nanometer sheet；The volume ratio of water and acetone is 1 in the mixed liquor of the acetone and water:(1~5)；It is described organic
The quality of high molecular polymer and the volume ratio of dihydric alcohol are 1g:(100~200) mL；The quality of the bismuth salt and dihydric alcohol
Volume ratio is 1g:(300~400) mL；The quality of the selenium source and the volume ratio of dihydric alcohol are 1g:(600~700) mL；It is described
The volume ratio of reducing agent and dihydric alcohol is 1mL:(60~70) mL；
2nd, dopamine hydrochloride is dissolved in Tri(Hydroxymethyl) Amino Methane Hydrochloride solution, after ultrasonic mixing is uniform, added thereto
Enter the Bi that step 1 obtains2Se3Nanometer sheet, after 3~9h of stirring at normal temperature, is centrifuged using deionized water and washs 3~5 times, Ran Houfen
Dissipate in dimethyl sulfoxide (DMSO), obtain Bi2Se3@PDA nanoparticle dispersion liquids；The Bi2Se3@PDA nanoparticle dispersion liquids it is dense
Spend for (2~4) mg/mL；The quality of the dopamine hydrochloride and the volume ratio of Tri(Hydroxymethyl) Amino Methane Hydrochloride solution are
1g:(300~500) mL；The Bi2Se3The quality of nanometer sheet and the volume ratio of Tri(Hydroxymethyl) Amino Methane Hydrochloride solution are
1g:(1500~2500) mL；The pH of the Tri(Hydroxymethyl) Amino Methane Hydrochloride solution is 8~10；
3rd, doxorubicin hydrochloride is dissolved in dimethyl sulfoxide (DMSO), after ultrasonic mixing is uniform, obtains doxorubicin hydrochloride dispersion liquid；By salt
Sour adriamycin dispersion liquid is added to the Bi that step 2 obtains2Se3In@PDA nanoparticle dispersion liquids, 5~15min of stirring at normal temperature, obtains
To mixed solution, then mixed solution is added drop-wise in seralbumin aqueous solution dropwise under ultrasound condition, using deionization
Water is centrifuged and washed 3~5 times, obtains solid, solid is scattered in phosphate buffer, is positioned over the refrigerator that temperature is 4 DEG C
Middle preservation, obtains multi-functional bismuth selenide nano-complex；The concentration of the doxorubicin hydrochloride dispersion liquid is (5~20) mg/mL；Institute
State doxorubicin hydrochloride dispersion liquid and Bi2Se3The volume ratio of@PDA nanoparticle dispersion liquids is 1:(1~4)；The mixed solution with
The volume ratio of seralbumin aqueous solution is 1:(2~6)；The quality of the solid and the volume ratio of phosphate buffer are 1g:
(1000~2000) mL；The concentration of seralbumin aqueous solution described in step 3 is 5.0mg/mL.
A kind of 2. preparation method of multi-functional bismuth selenide nano-complex according to claim 1, it is characterised in that step
Organic high molecular polymer described in one is the mixture of one or both of polyvinylpyrrolidone and polyacrylic acid.
A kind of 3. preparation method of multi-functional bismuth selenide nano-complex according to claim 1, it is characterised in that step
Dihydric alcohol described in one is one kind or wherein several mixtures in ethylene glycol, propane diols and butanediol.
A kind of 4. preparation method of multi-functional bismuth selenide nano-complex according to claim 1, it is characterised in that step
Selenium source described in one is one kind or wherein several mixtures in sodium selenite, potassium selenite and selenium dioxide；In step 1
The bismuth salt is one kind or wherein several mixtures in bismuth nitrate, bismuth chloride and bismuth acetate.
A kind of 5. preparation method of multi-functional bismuth selenide nano-complex according to claim 1, it is characterised in that step
Reducing agent described in one is mixing a kind of or wherein several in azanol, morpholine borine, sodium borohydride, glucose and ascorbic acid
Thing.
A kind of 6. preparation method of multi-functional bismuth selenide nano-complex according to claim 1, it is characterised in that step
The behaviour seralbumin aqueous solution of seralbumin aqueous solution described in three and one kind in Bovine Serum Albumin in Aqueous Solution or two
The mixture of kind.
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| CN107601437B (en) * | 2017-11-03 | 2019-05-07 | 安徽工业大学 | A kind of preparation method of selenic acid bismuth nanobelt |
| CN109985247A (en) * | 2019-04-03 | 2019-07-09 | 河南科技学院 | A kind of preparation method of the hybridized metal organic framework compounds for drug release |
| CN111171805A (en) * | 2020-01-09 | 2020-05-19 | 南方医科大学 | Shell layer bismuth-containing nano material and preparation method and application thereof |
| CN112281183B (en) * | 2020-09-16 | 2022-01-18 | 华南理工大学 | Cluster-shaped bismuth selenide, preparation method thereof and application of cluster-shaped bismuth selenide in electrocatalytic reduction of carbon dioxide |
| CN113082215B (en) * | 2021-04-19 | 2022-01-18 | 苏州大学 | Bismuth selenide hydrogel, bismuth selenide hydrogel bacterial carrier, preparation method and application |
| CN114477113B (en) * | 2021-12-15 | 2023-04-18 | 中国科学院深圳先进技术研究院 | Preparation and application of two-dimensional cuprous phosphide/bismuth selenide heterojunction composite material |
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