CN105434443B - A kind of mysterious fruit tree branch extract and its application in the medicine or health products for the treatment of diabetes or hyperlipemia is prepared - Google Patents
A kind of mysterious fruit tree branch extract and its application in the medicine or health products for the treatment of diabetes or hyperlipemia is prepared Download PDFInfo
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- 239000000284 extract Substances 0.000 title claims abstract description 80
- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 70
- 239000003814 drug Substances 0.000 title claims abstract description 29
- 206010012601 diabetes mellitus Diseases 0.000 title claims abstract description 16
- 201000005577 familial hyperlipidemia Diseases 0.000 title claims abstract description 8
- 239000004480 active ingredient Substances 0.000 claims abstract description 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical group CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 42
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 36
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical group CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 33
- -1 β-amyrin acetic acid esters Chemical class 0.000 claims description 31
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical group OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 30
- FSLPMRQHCOLESF-UHFFFAOYSA-N alpha-amyrenol Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C)CCC(C)C(C)C5C4=CCC3C21C FSLPMRQHCOLESF-UHFFFAOYSA-N 0.000 claims description 29
- QQFMRPIKDLHLKB-UHFFFAOYSA-N beta-amyrin Natural products CC1C2C3=CCC4C5(C)CCC(O)C(C)(C)C5CCC4(C)C3(C)CCC2(C)CCC1(C)C QQFMRPIKDLHLKB-UHFFFAOYSA-N 0.000 claims description 29
- PDNLMONKODEGSE-UHFFFAOYSA-N beta-amyrin acetate Natural products CC(=O)OC1CCC2(C)C(CCC3(C)C4(C)CCC5(C)CCC(C)(C)CC5C4=CCC23C)C1(C)C PDNLMONKODEGSE-UHFFFAOYSA-N 0.000 claims description 29
- 239000003208 petroleum Substances 0.000 claims description 26
- 238000010828 elution Methods 0.000 claims description 25
- 239000012046 mixed solvent Substances 0.000 claims description 25
- 125000004836 hexamethylene group Chemical group [H]C([H])([*:2])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[*:1] 0.000 claims description 23
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 22
- 239000002904 solvent Substances 0.000 claims description 20
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 13
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 claims description 12
- BQFCCCIRTOLPEF-UHFFFAOYSA-N chembl1976978 Chemical compound CC1=CC=CC=C1N=NC1=C(O)C=CC2=CC=CC=C12 BQFCCCIRTOLPEF-UHFFFAOYSA-N 0.000 claims description 11
- 239000012535 impurity Substances 0.000 claims description 11
- 239000003960 organic solvent Substances 0.000 claims description 10
- 239000007864 aqueous solution Substances 0.000 claims description 8
- 238000010898 silica gel chromatography Methods 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 7
- 239000000470 constituent Substances 0.000 claims description 4
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 239000012141 concentrate Substances 0.000 claims 1
- PKGKOZOYXQMJNG-UHFFFAOYSA-N lupeol Natural products CC(=C)C1CC2C(C)(CCC3C4(C)CCC5C(C)(C)C(O)CCC5(C)C4CCC23C)C1 PKGKOZOYXQMJNG-UHFFFAOYSA-N 0.000 claims 1
- 210000004369 blood Anatomy 0.000 abstract description 16
- 239000008280 blood Substances 0.000 abstract description 16
- 230000002218 hypoglycaemic effect Effects 0.000 abstract description 11
- 229940079593 drug Drugs 0.000 abstract description 10
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract description 8
- 102000004877 Insulin Human genes 0.000 abstract description 4
- 108090001061 Insulin Proteins 0.000 abstract description 4
- 229940125396 insulin Drugs 0.000 abstract description 4
- 150000002168 ethanoic acid esters Chemical class 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 description 24
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 19
- 239000000741 silica gel Substances 0.000 description 19
- 229910002027 silica gel Inorganic materials 0.000 description 19
- 229960001866 silicon dioxide Drugs 0.000 description 19
- 239000000469 ethanolic extract Substances 0.000 description 12
- 241000700159 Rattus Species 0.000 description 11
- HEDRZPFGACZZDS-MICDWDOJSA-N Trichloro(2H)methane Chemical compound [2H]C(Cl)(Cl)Cl HEDRZPFGACZZDS-MICDWDOJSA-N 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 239000012259 ether extract Substances 0.000 description 8
- 150000002632 lipids Chemical class 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 238000004128 high performance liquid chromatography Methods 0.000 description 7
- 206010013786 Dry skin Diseases 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 6
- 238000012856 packing Methods 0.000 description 6
- 238000010992 reflux Methods 0.000 description 6
- 238000005303 weighing Methods 0.000 description 6
- 235000011341 Sideroxylon dulcificum Nutrition 0.000 description 5
- 241000220220 Synsepalum dulcificum Species 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 235000005266 Thaumatococcus daniellii Nutrition 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 201000001421 hyperglycemia Diseases 0.000 description 3
- PCZOHLXUXFIOCF-BXMDZJJMSA-N lovastatin Chemical group C([C@H]1[C@@H](C)C=CC2=C[C@H](C)C[C@@H]([C@H]12)OC(=O)[C@@H](C)CC)C[C@@H]1C[C@@H](O)CC(=O)O1 PCZOHLXUXFIOCF-BXMDZJJMSA-N 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000003595 spectral effect Effects 0.000 description 3
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 2
- 238000005160 1H NMR spectroscopy Methods 0.000 description 2
- PCZOHLXUXFIOCF-UHFFFAOYSA-N Monacolin X Natural products C12C(OC(=O)C(C)CC)CC(C)C=C2C=CC(C)C1CCC1CC(O)CC(=O)O1 PCZOHLXUXFIOCF-UHFFFAOYSA-N 0.000 description 2
- ZSJLQEPLLKMAKR-UHFFFAOYSA-N Streptozotocin Natural products O=NN(C)C(=O)NC1C(O)OC(CO)C(O)C1O ZSJLQEPLLKMAKR-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 239000002026 chloroform extract Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000003304 gavage Methods 0.000 description 2
- 238000002114 high-resolution electrospray ionisation mass spectrometry Methods 0.000 description 2
- 229960004844 lovastatin Drugs 0.000 description 2
- QLJODMDSTUBWDW-UHFFFAOYSA-N lovastatin hydroxy acid Natural products C1=CC(C)C(CCC(O)CC(O)CC(O)=O)C2C(OC(=O)C(C)CC)CC(C)C=C21 QLJODMDSTUBWDW-UHFFFAOYSA-N 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 description 2
- 229960001052 streptozocin Drugs 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- KXIZXPRLNNDQKS-UHFFFAOYSA-N 2-[3,4-bis(phenylmethoxy)phenyl]ethanamine;hydron;chloride Chemical compound Cl.C=1C=CC=CC=1COC1=CC(CCN)=CC=C1OCC1=CC=CC=C1 KXIZXPRLNNDQKS-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- ODSSDTBFHAYYMD-FYRRWYBVSA-N Lupeol acetate Natural products O=C(O[C@@H]1C(C)(C)[C@H]2[C@@](C)([C@@H]3[C@](C)([C@@]4(C)[C@@H]([C@@H]5[C@@H](C(=C)C)CC[C@]5(C)CC4)CC3)CC2)CC1)C ODSSDTBFHAYYMD-FYRRWYBVSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 229960002708 antigout preparations Drugs 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 210000001508 eye Anatomy 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 238000007446 glucose tolerance test Methods 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 230000000055 hyoplipidemic effect Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000003914 insulin secretion Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 235000021590 normal diet Nutrition 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Public Health (AREA)
- Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to Chinese medicine and natural medicine technical field, specifically discloses a kind of mysterious fruit tree branch extract and its application in the medicine or health products for the treatment of diabetes or hyperlipemia is prepared.Described mysterious fruit tree branch extract includes active ingredient β amyrins acetic acid esters and/or Thin Layer scanning.Described mysterious fruit tree branch extract has the function that insulin content in hypoglycemic well and lifting blood, can be used as the medicine or health products for the treatment of diabetes；And described extract active ingredient is clear and definite, be advantageous to control drug quality and drug safety.
Description
Technical field
The present invention relates to Chinese medicine and natural medicine technical field, and in particular to a kind of mysterious fruit tree branch extract and its is making
Application in the medicine or health products of standby treatment diabetes or hyperlipemia.
Background technology
Diabetes are one group of metabolic diseases characterized by hyperglycaemia.Hyperglycaemia be due to then defect of insulin secretion or
Its biological agent is damaged, or both have concurrently and cause.Long-standing hyperglycaemia during diabetes, it can cause each
Kind tissue, particularly eye, kidney, heart, blood vessel, the chronic lesion of nerve, dysfunction.Therefore, a kind of sugar of exploitation
Disease medicine tool is urinated to be of great significance.
Miracle fruit, its Classification system are Synsepalum dulcificum Daniell, are typical tropical evergreen wood,
Original producton location is in West Africa, Ghana, the band of the Congo one.In the 1960s, Premier Zhou Enlai to West Africa access when,
Republic of Ghana gives premier Zhou using miracle fruit as state's gift.Hereafter, miracle fruit starts to cultivate in China.
Patent CN102743421A discloses a kind of miracle fruit fruit tree branch leaf extract, it was recently reported that its have it is anti-oxidant and
Antigout acts on.But it there is no and report that mysterious fruit tree branch has hypoglycemic effect, more prepared without using modernization of Chinese medicine technology
Active component and clearly it is main effectively into report.Therefore, a kind of medicine for treating diabetes, tool are developed using mysterious fruit tree branch
It is of great significance.
The content of the invention
The technical problems to be solved by the invention are to treat the medicament categories of diabetes not in the prior art to overcome
A kind of foot, there is provided mysterious fruit tree branch extract.
Above-mentioned technical problem to be solved by this invention, is achieved by the following technical programs：
A kind of mysterious fruit tree branch extract, includes active ingredient β-amyrin acetic acid esters and/or Thin Layer scanning.
Inventor, by substantial amounts of experimental study, attempted to be permitted to develop a kind of medicine for treating diabetes
Several kinds of Chinese medicinal materials, it experienced and many times fail, successfully develop said extracted thing finally.It the experiment proved that, above-mentioned mysterious fruit tree
Branch extract has extraordinary treatment diabetes effect, and active ingredient therein is clear and definite, be advantageous to control drug quality with
And drug safety.
Described β-amyrin acetic acid esters（Entitled β-amyin the acetate of English）And Thin Layer scanning（English
Entitled lupeol acetate）Structural formula is as follows：
Preferably, the weight sum of described β-amyrin acetic acid esters and Thin Layer scanning accounts for extract gross weight
20% ~ 99%.Through embodiment it is demonstrated experimentally that β-amyrin acetic acid esters and Thin Layer scanning monomer have hypoglycemic work
With as long as therefore there is hypoglycemic effect, and its containing β-amyrin acetic acid esters and Thin Layer scanning in extract
Content is higher, and effect is better.As the two content can be selected from 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%.
It is highly preferred that the weight sum of described β-amyrin acetic acid esters and Thin Layer scanning accounts for extract gross weight
The 50% ~ 99% of amount.
Preferably, the weight of β-amyrin acetic acid esters accounts for extract gross weight in described mysterious fruit tree branch extract
5%~99%。
It is highly preferred that the weight of described β-amyrin acetic acid esters accounts for the 30% ~ 99% of extract gross weight.
It is further preferred that the weight of described β-amyrin acetic acid esters accounts for the 40% ~ 99% of extract gross weight.
Preferably, the weight of Thin Layer scanning accounts for the 5% of extract gross weight in described mysterious fruit tree branch extract
~99%。
It is highly preferred that the weight of described Thin Layer scanning accounts for the 30% ~ 99% of extract gross weight.
It is further preferred that the weight of described Thin Layer scanning accounts for the 40% ~ 99% of extract gross weight.
The present invention also provides a kind of preparation method of mysterious fruit tree branch extract, comprises the following steps：
S1. mysterious fruit tree branch is extracted with organic solvent first, obtains extractive with organic solvent；
S2. by extractive with organic solvent through any one solvent in petroleum ether, dichloromethane, chloroform and hexamethylene or
Mixed extractant solvent more than any two obtains extract；
S3. extract is produced through silica gel column chromatography is active constituent-enriched；The condition of described silica gel column chromatography is：It is first
It is first 95 with volume ratio:5~80:20 solvent orange 2 A：The mixed solvent system of solvent B compositions carries out elution removal of impurities；Then body is used again
Product is than being 70:30~50:50 solvent orange 2 A：The mixed solvent system of solvent B compositions is eluted and collects elution position, and concentration is dry
It is dry to produce described mysterious fruit tree branch extract；Described solvent orange 2 A is selected from petroleum ether, dichloromethane, chloroform or hexamethylene, described
Solvent B be selected from ethyl acetate, acetone or methanol.
Preferably, the condition of the silica gel column chromatography described in step S3 is：Volume ratio is 90 first:10~85:15 solvent
A：The mixed solvent system of solvent B compositions carries out elution removal of impurities；Then it is 70 to use volume ratio again:30~60:40 solvent orange 2 A：Solvent
The mixed solvent system of B compositions is eluted and collects elution position, is concentrated and dried and is produced described mysterious fruit tree branch extract.
It is further preferred that described solvent orange 2 A：The mixed solvent system of solvent B compositions is hexamethylene：Acetone composition mixes
Bonding solvent system.
Most preferably, the condition of the silica gel column chromatography described in step S3 is：It is 85 first with volume ratio:15 hexamethylene
Alkane：The mixed solvent system of acetone composition carries out elution removal of impurities；Then it is 70 to use volume ratio again:30 hexamethylene：Acetone forms
Mixed solvent system eluted and collect elution position, be concentrated and dried and produce described mysterious fruit tree branch extract.
Preferably, the organic solvent described in step S1 is methanol or ethanol.
It is highly preferred that it is 70% ~ 100% methanol aqueous solution or ethanol water that the organic solvent described in step S1, which is volume fraction,
Solution.
The present invention also provides above-mentioned mysterious fruit tree branch extract and is preparing medicine or the guarantor for the treatment of diabetes or hyperlipemia
Application in strong product.
After the extract hypoglycemic activity is tested, inventor is also tested for the hypolipidemic activity of the extract simultaneously,
As a result find, the extract simultaneously also there is good reducing blood lipid and norcholesterol to act on, and can be used as treatment hyperlipemia
Medicine or health products use.
Beneficial effect：（1）The invention provides a kind of brand-new mysterious fruit tree branch extraction with treatment diabetes effect
Thing, described extract have the function that insulin content in hypoglycemic well and lifting blood, can be used as treatment sugar
The medicine or health products for urinating disease use；（2）In addition, also confirm that extract of the present invention also has drop well by experiment
Blood fat and norcholesterol effect, it can be used as the medicine for the treatment of hyperlipemia；（3）Described extract specify that wherein
Active ingredient, be advantageous to control drug quality and drug safety.
Embodiment
Be explained further the present invention below in conjunction with embodiment, but embodiment the present invention is not done it is any type of
Limit.
The preparation of 1 β of embodiment-amyrin acetic acid esters and Thin Layer scanning
S1. the mysterious fruit tree branch for weighing 2Kg dryings crushes, and crosses 60 mesh sieves, then with the ethanol that 10L volume fractions are 95%
Aqueous solution heating and refluxing extraction, extraction time are 2 times, each 1h, and concentrated extracting solution obtains mysterious fruit tree branch ethanol extract；
S2. add 2L water to be well mixed into mysterious fruit tree branch ethanol extract, add petroleum ether extraction, extraction 3
Secondary, the dosage of each petroleum ether is 2L, is concentrated under reduced pressure and removes petroleum ether, obtains petroleum ether extract；
S3. petroleum ether extract 40g is taken, is dissolved with ethyl acetate, then sample is mixed with 40g silica gel (100 mesh), separately takes
800g silica gel (300 mesh) dry column-packing, sample is loaded on silicagel column, be first 85 with volume ratio:15 hexamethylene：Acetone mixes
Bonding solvent elution removal of impurities；It is again 70 with volume ratio:30 hexamethylene：Acetone mixed solvent elutes, and collects eluent and is concentrated by
TLC plates, which are examined, finds that elution position contains 2 main compounds；
S4. the elution position containing 2 main compounds obtained through step S3 is further separated, separation method is
Using silicagel column in hexamethylene：Column chromatography for separation is carried out repeatedly in acetone mixed solvent system and obtains obtaining 2 principal monomer chemical combination
Thing；Through further Spectral Identification, it is respectively β-amyrin acetic acid esters and Thin Layer scanning to obtain two compounds；
The spectral data of β-amyrin acetic acid esters is as follows：HRESIMSm/z 469.4053 [M+H]+ (calcd for
C32H53O2, 469.4040). 1H NMR (400 MHz, CDCl3)δ 0.86 (3H, s, H-29), 1.13 (3H, s,
H-28), 0.86 (3H, s, H-27), 0.83 (3H, s, H-26), 0.97 (3H, s, H-25), 0.96 (3H,
s, H-24), 0.87 (3H, s, H-23), 2.05 (3H, s, COCH3), 4.50 (1H, m, H-3), 5.18
(1H, t, J = 7.6, H-12).13C NMR (100 MHz, CDCl3)δ 38.4 (C-1), 23.8 (C-2), 81.1
(C-3), 37.8 (C-4), 55.4 (C-5), 18.4 (C-6), 32.7 (C-7), 39.9 (C-8), 47.7 (C-
9), 37.0 (C-10), 23.6 (C-11), 121.7 (C-12), 145.3 (C-13), 41.8 (C-14), 28.5
(C-15), 26.2 (C-16), 32.6 (C-17), 47.3 (C-18), 46.9 (C-19), 31.2 (C-20), 34.8
(C-21), 37.3 (C-22), 28.1 (C-23), 16.8 (C-24), 15.7 (C-25), 16.9 (C-26),26.1
(C-27), 27.0 (C-28), 33.4 (C-29), 23.7 (C-30), 171.1 (C-1`), 21.4 (C-2`).
The spectral data of Thin Layer scanning is as follows：HRESIMSm/z 469.4029 [M+H]+ (calcd for
C32H53O2, 469.4040).1H NMR (400 MHz, CDCl3)δ 1.66 (3H, s, H-30), 1.02 (3H, s,
H-28), 0.78 (3H, s, H-27), 0.84 (3H, s, H-26), 1.00 (3H, s, H-25), 0.95 (3H,
s, H-24), 0.84 (3H, s, H-23), 2.03 (3H, s, COCH3), 4.46 (1H, m, H-3), 4.68
(1H, dd, J =2.4 Hz, H-29a), 4.56 (1H, dd, J = 2.4 Hz, H-29b).13C NMR (100 MHz,
CDCl3)δ 38.5 (C-1), 23.8 (C-2), 81.1 (C-3), 37.9 (C-4), 55.5 (C-5), 18.3 (C-
6), 34.3 (C-7), 40.9 (C-8), 50.4 (C-9), 37.2 (C-10), 21.1 (C-11), 25.2 (C-
12), 38.1 (C-13), 42.9 (C-14), 27.5 (C-15), 35.7 (C-16), 43.1 (C-17), 48.4
(C-18), 48.1 (C-19), 151.0 (C-20), 29.9 (C-21), 40.1 (C-22), 28.0 (C-23),
16.6 (C-24), 16.3 (C-25), 16.1 (C-26), 14.6 (C-27), 18.1 (C-28), 109.4 (C-
29), 19.4 (C-30), 171.1 (C-1`), 21.4 (C-2`).
The preparation of the mysterious fruit tree branch extract of embodiment 2
S1. the mysterious fruit tree branch for weighing 2Kg dryings crushes, and crosses 60 mesh sieves, then with the ethanol that 10L volume fractions are 95%
Aqueous solution heating and refluxing extraction, extraction time are 2 times, each 1h, and concentrated extracting solution obtains mysterious fruit tree branch ethanol extract；
S2. add 2L water to be well mixed into mysterious fruit tree branch ethanol extract, add petroleum ether extraction, extraction 3
Secondary, the dosage of each petroleum ether is 2L, is concentrated under reduced pressure and removes petroleum ether, obtains petroleum ether extract；
S3. petroleum ether extract 40g is taken, is dissolved with ethyl acetate, then sample is mixed with 40g silica gel (100 mesh), separately takes
800g silica gel (300 mesh) dry column-packing, sample is loaded on silicagel column, be first 85 with volume ratio:15 hexamethylene：Acetone mixes
Bonding solvent elution removal of impurities；It is again 70 with volume ratio:30 hexamethylene：Acetone mixed solvent elutes, and collects elution position, and concentration is dry
It is dry to obtain mysterious fruit tree branch extract.
Analyzed through HPLC, β-amyrin acetic acid esters and Thin Layer scanning always contains in the mysterious fruit tree branch extract
Measure as 78%.The content of wherein β-amyrin acetic acid esters is 41%, the content of Thin Layer scanning is 37%.
The preparation of the mysterious fruit tree branch extract of embodiment 3
S1. the mysterious fruit tree branch for weighing 2Kg dryings crushes, and crosses 60 mesh sieves, then with the ethanol that 10L volume fractions are 95%
Aqueous solution heating and refluxing extraction, extraction time are 2 times, each 1h, and concentrated extracting solution obtains mysterious fruit tree branch ethanol extract；
S2. add 2L water to be well mixed into mysterious fruit tree branch ethanol extract, add petroleum ether extraction, extraction 3
Secondary, the dosage of each petroleum ether is 2L, is concentrated under reduced pressure and removes petroleum ether, obtains petroleum ether extract；
S3. petroleum ether extract 40g is taken, is dissolved with ethyl acetate, then sample is mixed with 40g silica gel (100 mesh), separately takes
800g silica gel (300 mesh) dry column-packing, sample is loaded on silicagel column, be first 90 with volume ratio:10 hexamethylene：Acetone mixes
Bonding solvent elution removal of impurities；It is again 60 with volume ratio:40 hexamethylene：Acetone mixed solvent elutes, and collects elution position, and concentration is dry
It is dry to obtain mysterious fruit tree branch extract.
Analyzed through HPLC, β-amyrin acetic acid esters and Thin Layer scanning always contains in the mysterious fruit tree branch extract
Measure as 62%.The content of wherein β-amyrin acetic acid esters is 39%, the content of Thin Layer scanning is 23%.
The preparation of the mysterious fruit tree branch extract of embodiment 4
S1. the mysterious fruit tree branch for weighing 2Kg dryings crushes, and crosses 60 mesh sieves, then with the ethanol that 10L volume fractions are 95%
Aqueous solution heating and refluxing extraction, extraction time are 2 times, each 1h, and concentrated extracting solution obtains mysterious fruit tree branch ethanol extract；
S2. add 2L water to be well mixed into mysterious fruit tree branch ethanol extract, add chloroform extraction, extract 3 times,
The dosage of each chloroform is 2L, is concentrated under reduced pressure and removes chloroform, obtains chloroform extract；
S3. chloroform extract 40 is taken, is dissolved with methanol, then sample is mixed with 40g silica gel (100 mesh), separately takes 800g silica gel
(300 mesh) dry column-packing, sample is loaded on silicagel column, be first 95 with volume ratio:5 chloroform：Methanol mixed solvent elution removes
It is miscellaneous；It is again 60 with volume ratio:40 chloroform：Methanol mixed solvent elutes, and collects elution position, is concentrated and dried to obtain mysterious fruit tree branch
Extract.
Analyzed through HPLC, β-amyrin acetic acid esters and Thin Layer scanning always contains in the mysterious fruit tree branch extract
Measure as 51%.The content of wherein β-amyrin acetic acid esters is 19%, the content of Thin Layer scanning is 32%.
The preparation of the mysterious fruit tree branch extract of embodiment 5
S1. the mysterious fruit tree branch for weighing 2Kg dryings crushes, and crosses 60 mesh sieves, then with the ethanol that 10L volume fractions are 95%
Aqueous solution heating and refluxing extraction, extraction time are 2 times, each 1h, and concentrated extracting solution obtains mysterious fruit tree branch ethanol extract；
S2. add 2L water to be well mixed into mysterious fruit tree branch ethanol extract, add hexamethylene extraction, extraction 3
Secondary, the dosage of each hexamethylene is 2L, is concentrated under reduced pressure and removes hexamethylene, obtains hexamethylene extract；
S3. hexamethylene extract 40 is taken, is dissolved with ethyl acetate, then sample is mixed with 40g silica gel (100 mesh), separately takes
800g silica gel (300 mesh) dry column-packing, sample is loaded on silicagel column, be first 95 with volume ratio:5 hexamethylene：Ethyl acetate
Mixed solvent elution removal of impurities；It is again 60 with volume ratio:40 hexamethylene：Ethyl acetate mixed solvent elutes, and collects elution position,
It is concentrated and dried to obtain mysterious fruit tree branch extract.
Analyzed through HPLC, β-amyrin acetic acid esters and Thin Layer scanning always contains in the mysterious fruit tree branch extract
Measure as 43%.The content of wherein β-amyrin acetic acid esters is 25%, the content of Thin Layer scanning is 18%.
The preparation of the mysterious fruit tree branch extract of embodiment 6
S1. the mysterious fruit tree branch for weighing 2Kg dryings crushes, and crosses 60 mesh sieves, then with the ethanol that 10L volume fractions are 95%
Aqueous solution heating and refluxing extraction, extraction time are 2 times, each 1h, and concentrated extracting solution obtains mysterious fruit tree branch ethanol extract；
S2. add 2L water to be well mixed into mysterious fruit tree branch ethanol extract, add petroleum ether extraction, extraction 3
Secondary, the dosage of each petroleum ether is 2L, is concentrated under reduced pressure and removes petroleum ether, obtains petroleum ether extract；
S3. petroleum ether extract 40 is taken, is dissolved with ethyl acetate, then sample is mixed with 40g silica gel (100 mesh), separately takes
800g silica gel (300 mesh) dry column-packing, sample is loaded on silicagel column, be first 95 with volume ratio:5 petroleum ether：Ethyl acetate
Mixed solvent elution removal of impurities；It is again 50 with volume ratio:50 petroleum ether：Ethyl acetate mixed solvent elutes, and collects elution position,
It is concentrated and dried to obtain mysterious fruit tree branch extract.
Analyzed through HPLC, β-amyrin acetic acid esters and Thin Layer scanning always contains in the mysterious fruit tree branch extract
Measure as 25%.The content of wherein β-amyrin acetic acid esters is 13%, the content of Thin Layer scanning is 12%.
The content percentage of above-mentioned β-amyrin acetic acid esters and Thin Layer scanning is weight content percentage, content
The method of analysis is HPLC methods, and HPLC analysis condition is：Mobile phase is the volume % water of 90 volume % methanol -10, wavelength 254
Nm, chromatographic column are cosmosil 5C18-MS-II column, and 4.6 x250nm, column temperature is 25 degrees Celsius；Set respectively with β-perfume
Lipidol acetic acid esters and Thin Layer scanning are that standard items draw peak area-concentration standard curve, then with analysis extract
β-amyrin acetic acid esters and Thin Layer scanning peak area, and respective content is calculated according to standard curve.
The mysterious hypoglycemic experiment of fruit tree branch extract of embodiment 7
By 108 male SD rats (4 week old cleaning grades, 150~180g of body weight, by Guangdong Medical Lab Animal Center
There is provided) Normal group 12 and diabetic model group 96 are randomly divided into, normal group gives basal feed, and model group is given
High lipid food (lard 18%, sucrose 20%, yolk 3%, basal feed 59%).After feeding 4 weeks, after fasting 12h,
STZ (Streptozotocin) is made into 2 weight % solution by 0.1mmol/L sodium citrate buffers (pH=4.2), by 35mg/
Kg is injected intraperitoneally, and rat 2h Glucose tolerance tests are surveyed after one week, and (fasting 12h, gavage give 2g/kg glucose, after surveying gavage
2h blood glucose), rat of the screening blood glucose value more than 11.1mmol/L is defined as DM rats.By into the DM rats of mould after stablizing one week
96 are randomly divided into 8 groups（Model group and experimental group）.Experimental group dosage is 2mg/kg, every group each 12.The use of each experimental group
Medicine situation is as follows：Experimental group 1（Individually use β-amyrin acetic acid esters）, experimental group 2（Individually use Thin Layer scanning）, experiment
Group 3（The mysterious fruit tree branch extract that embodiment 2 is prepared）, experimental group 4（The mysterious fruit tree branch that embodiment 3 is prepared carries
Take thing）, experimental group 5（The mysterious fruit tree branch extract that embodiment 4 is prepared）, experimental group 6（The god that embodiment 5 is prepared
Secret fruit tree branch extract）, experimental group 7（The mysterious fruit tree branch extract that embodiment 6 is prepared）.Gastric infusion 1 time daily, even
It is continuous 6 weeks.Weighed in 1 time per weekend, dosage is adjusted according to body weight.Terminate first 1 day in experiment, Rat Fast 12h, take blood
Survey fasting blood-glucose (FBG), FPI (FIN).Result of the test is shown in Table 1：
* p ＜ 005, * * p ＜ 001, model group vs Normal groups；#P ＜ 005,##P ＜ 001, experimental group vs
Model group.
From the results shown in Table 1, compared with Normal group, model group fasting blood glucose level significantly raises (p ＜
0.01), FPI significantly reduces (p ＜ 0.01), illustrates to model successfully.
It can be seen that from the fasting blood-glucose in experimental group 1 and 2 and FPI data and separated from mysterious fruit tree branch
To β-amyrin acetic acid esters and Thin Layer scanning be respectively provided with it is hypoglycemic and lifting blood in insulin content work
With especially β-amyrin acetic acid esters has good hypoglycemic effect.
From experimental group 3 ~ 7 as can be seen that the mysterious fruit tree branch extract that the present invention is prepared all has drop blood well
The effect of insulin content in sugar and lifting blood.This illustrates that extract of the present invention can be as treatment diabetes
Medicine or health products use.It can be seen that active constituent content in extract is higher from its hypoglycemic data, hypoglycemic effect
Effect is better.
The lipid-lowering test of embodiment 8
SD rats 50 are taken, male and female half and half, are divided into normal group (feeding normal diet), high lipid food group, experimental group 1（Implement
The extract that example 2 is prepared）, experimental group 2（The extract that embodiment 6 is prepared）, positive controls（Lovastatin）Often
Group is respectively 10, and high lipid food group, experimental group and positive controls feed high lipid food, and experimental group 1,2 is pressed daily simultaneously
3mg/kg amount feeds the extract that corresponding embodiment is prepared, and positive controls feed Lovastatin by 3mg/kg amount daily.
Each group rat, free water are fed by group, successive administration puts to death animal after 35 days, and abdominal vein takes blood, determines
Blood fat.Serum total cholesterol is determined on automatic clinical chemistry analyzer with enzyme process, the precipitation method（TC）, triglycerides（TG）Contain
Amount.Experimental result is as shown in table 2.
From Table 2, it can be seen that TC the and TG concentration of high lipid food group rat blood serum is all remarkably higher than chow diet group (P<
0.01), illustrate to model successfully.
Experimental group 1, the serum TC of 2 rats, TG concentration are significantly lower than high lipid food group (P<0.05) present invention, is illustrated
Extract, which is prepared, has the function that effect for reducing blood fat and norcholesterol, and therefore, extract of the present invention can be used as treatment high
The medicine of blood fat disease uses.It can also be seen that experimental group 1 is to the serum TC of rat from table 2, TG concentration reduces more bright
It is aobvious and suitable with positive control drug Lovastatin, illustrate that the active constituent content in extract of the present invention is higher, its reducing blood lipid with
And the effect of norcholesterol is better.
Claims (9)
1. a kind of mysterious fruit tree branch extract, it is characterised in that include active ingredient β-amyrin acetic acid esters and lupeol
Acetic acid esters；
The weight sum of described β-amyrin acetic acid esters and Thin Layer scanning accounts for the 20% ~ 99% of extract gross weight.
2. mysterious fruit tree branch extract according to claim 1, it is characterised in that the weight of β-amyrin acetic acid esters accounts for
The 5% ~ 99% of extract gross weight；The weight of the Thin Layer scanning accounts for the 5% ~ 99% of extract gross weight.
3. the preparation method of the mysterious fruit tree branch extract described in claim 1 or 2, it is characterised in that comprise the following steps：
S1. mysterious fruit tree branch is extracted with organic solvent first, obtains extractive with organic solvent；
S2. by extractive with organic solvent through any one solvent in petroleum ether, dichloromethane, chloroform and hexamethylene or any
More than two kinds of mixed extractant solvent obtains extract；
S3. extract is produced through silica gel column chromatography is active constituent-enriched；The condition of described silica gel column chromatography is：Use first
Volume ratio is 95:5~80:20 solvent orange 2 A：The mixed solvent system of solvent B compositions carries out elution removal of impurities；Then volume ratio is used again
For 70:30~50:50 solvent orange 2 A：The mixed solvent system of solvent B compositions is eluted and collects elution position, and concentrate drying is
Obtain described mysterious fruit tree branch extract；Described solvent orange 2 A is selected from petroleum ether, dichloromethane, chloroform or hexamethylene, and described is molten
Agent B is selected from ethyl acetate, acetone or methanol.
4. preparation method according to claim 3, it is characterised in that the condition of the silica gel column chromatography described in step S3
For：It is 90 first with volume ratio:10~85:15 solvent orange 2 A：The mixed solvent system of solvent B compositions carries out elution removal of impurities；Then
It is again 70 with volume ratio:30~60:40 solvent orange 2 A：The mixed solvent system of solvent B compositions is eluted and collects elution position,
It is concentrated and dried and produces described mysterious fruit tree branch extract.
5. preparation method according to claim 3, it is characterised in that described solvent orange 2 A：The mixed solvent of solvent B compositions
System is hexamethylene：The mixed solvent system of acetone composition.
6. preparation method according to claim 3, it is characterised in that the condition of the silica gel column chromatography described in step S3
For：It is 85 first with volume ratio:15 hexamethylene：The mixed solvent system of acetone composition carries out elution removal of impurities；Then body is used again
Product is than being 70:30 hexamethylene：The mixed solvent system of acetone composition is eluted and collects elution position, is concentrated and dried and is produced
Described mysterious fruit tree branch extract.
7. preparation method according to claim 3, it is characterised in that the organic solvent described in step S1 is methanol or second
Alcohol.
8. preparation method according to claim 7, it is characterised in that the organic solvent described in step S1 is volume fraction
For 75% ~ 100% methanol aqueous solution or ethanol water.
9. the mysterious fruit tree branch extract described in claim 1 is preparing the medicine or health products for the treatment of diabetes or hyperlipemia
In application.
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| US20080103201A1 (en) * | 2006-10-26 | 2008-05-01 | Wijayabandara Mirihanage Don J | Novel alpha-Glucosidase inhibitor from Tabernaemontana dichotoma |
| CN104725456A (en) * | 2013-12-24 | 2015-06-24 | 中国药科大学 | Pentacyclic triterpenoid cholesterol ester transfer protein (CETP) inhibitor, pharmaceutical composition thereof and medical application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080103201A1 (en) * | 2006-10-26 | 2008-05-01 | Wijayabandara Mirihanage Don J | Novel alpha-Glucosidase inhibitor from Tabernaemontana dichotoma |
| CN104725456A (en) * | 2013-12-24 | 2015-06-24 | 中国药科大学 | Pentacyclic triterpenoid cholesterol ester transfer protein (CETP) inhibitor, pharmaceutical composition thereof and medical application |
Non-Patent Citations (1)
| Title |
|---|
| 神秘果提取物对链脲佐菌素诱导的糖尿病大鼠血糖及脂代谢的影响;李彦等;《中药药理与临床》;20111231;第27卷(第4期);第64-67页 * |
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