CN105424919B - A kind of method based on GH and the gene blood biochemical markers seed selection Wuding Chickens of IGF 1 - Google Patents
A kind of method based on GH and the gene blood biochemical markers seed selection Wuding Chickens of IGF 1 Download PDFInfo
- Publication number
- CN105424919B CN105424919B CN201511017339.3A CN201511017339A CN105424919B CN 105424919 B CN105424919 B CN 105424919B CN 201511017339 A CN201511017339 A CN 201511017339A CN 105424919 B CN105424919 B CN 105424919B
- Authority
- CN
- China
- Prior art keywords
- wuding
- chicken
- igf
- wuding chicken
- elongated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of method based on GH and the gene blood biochemical markers seed selection Wuding Chickens of IGF 1, belong to poultry blood biochemical markers technical field, it is concretely comprised the following steps：1）The blood of Wuding Chicken to be measured is gathered, serum is prepared, the concentration value of GH, IGF 1 in detection serum；2）By step 1）The concentration value for measuring is contrasted with Wuding Chicken blood biochemical markers, and Wuding Chicken to be measured is distinguished as into fast elongated Wuding Chicken and slow elongated Wuding Chicken.The present invention is by determining change in concentration of the related gene GH and IGF 1 in slow elongated and fast elongated Wuding Chicken on growth axis, with reference to the body weight value of the Wuding Chicken of measurement, set up blood biochemical markers, for the early stage breeding of Wuding Chicken is provided fundamental basis, it is also simultaneously to explore Wuding County's a breed of chicken new method, is that Yunnan local chicken breeds provide new approaches in breeding work.
Description
Technical field
The invention belongs to poultry blood biochemical markers technical field, specifically, it is related to a kind of based on GH and IGF-1 genes
The method of blood biochemical markers seed selection Wuding Chicken.
Background technology
Poultry grow it is affected by various factors, and with the development of domestic fowl farming, to the continuous of chicken increment study
Deeply, it was found that the gene related to growing, GH, GH acceptor, GHBP, IGF-1, IGF-2, IGF acceptor and 6 IGF knots
Hop protein (IGFBP), gene on these growth axis is all to participate in poultry to grow essential part, wherein GH
With the change of IGF-1 concentration levels, the difference of growth speed is determined.
Growth hormone (GH) gene is the important hormone of animal growth regulation, and Ruqian Zhao wait (2003) to study
Show, the stage after early stage hatches, the strain of the chicken for more rapidly increasing shows relatively low GH cyclical levels.In D5, D21, D42
Laying hen and broiler chicken blood plasma in by observing influence of the pulsating nature (content) of GH to body weight, show that broiler chicken starts always in D5
Higher than laying hen, in D21, D42,4 and 5 times of body weight has even more been respectively reached in laying hen.
IGF -1 (IGF-1) gene is the most important gene that the poultry speed of growth is influenceed on growth axis
One of, Yakar S, waiting (2010) to study confirms that the loss of blood plasma IGF-1 causes of short and small stature and bone very thin and crisp, Yi Ji
After increased body weight, there is important meaning to the adaptability of bone.These reports have all convincingly demonstrated dependency basis on growth axis
Because of the critical role occupied in the growing of animal.
Body weight is quantitative character, is in dynamic change, its genetic progress in growing by the control of minor-polygene system
Slowly.Therefore marker assisted selection is to improve the effective way of chicken growth traits genetic progress, by determining growth axis dependency basis
Because of the concentration level in Wuding Chicken body, find and the closely related blood biochemical markers of Wuding Chicken body weight, accelerate educating for early stage
The process of kind.
At present, the blood biochemical markers as chicken growth traits about related gene on Wuding, Yunnan chicken growth axis are there is no,
To accelerate the breeding process of Wuding Chicken early stage, demand of the people to Local chicken breeds is met, it is necessary to inquire into a kind of energy and Wuding Chicken
The closely related blood biochemical markers of body weight, there is provided the new method of the fast elongated Wuding, Yunnan chicken of seed selection.
The content of the invention
Regarding to the issue above, the present invention proposes a kind of based on GH and IGF-1 gene blood biochemical markers seed selection Wuding Chickens
Method, by determining change in concentration of the related gene GH and IGF-1 in slow elongated and fast elongated Wuding Chicken on growth axis, with reference to
The body weight value of the Wuding Chicken of measurement, sets up blood biochemical markers, is that the early stage breeding of Wuding Chicken is provided fundamental basis, while being also
Wuding County's a breed of chicken new method is explored, is that Yunnan local chicken breeds provide new approaches in breeding work.
To achieve the above object, the present invention is achieved through the following technical solutions：
A kind of method based on GH and IGF-1 gene blood biochemical markers seed selection Wuding Chickens, concretely comprises the following steps：
1) blood of Wuding Chicken to be measured is gathered, serum is prepared, the concentration value of GH, IGF-1 in detection serum；
2) by step 1) concentration value and the Wuding Chicken blood biochemical markers that measure are contrasted, Wuding Chicken to be measured are distinguished as
Fast elongated Wuding Chicken and slow elongated Wuding Chicken；The estblishing step of the Wuding Chicken blood biochemical markers is：
A, the slow Wuding Chicken family long of random selection and fast Wuding Chicken family long from Wuding Chicken sources group, measure body respectively
Weight, gathers blood, collects blood plasma；
B, carried out by step from GH, GHBP and IGF-1 enzyme-linked immunoassay kit, determined using double antibody sandwich method
The level of the related gene of chicken in sample, then with ELIASA under 450nm wavelength mensuration absorbance, finally by standard curve
Calculate the content of GH, GHBP and IGF-1 in sample；
C, according to step b the data obtaineds, draw the dense of slow elongated Wuding Chicken and fast each growth related gene of elongated Wuding Chicken
Angle value, further according to the body weight value of selected Wuding Chicken, carries out the correlation analysis of growth related gene and body weight；
D, the blood biochemical markers that Wuding Chicken is determined according to the analysis result obtained by step c.
Further, the blood biochemical markers of described Wuding, Yunnan chicken are the concentration value of GH, IGF-1 gene, in January
During age, when the concentration value of GH genes is in 2.83 ± more than 0.03ug/l, and IGF-1 genes concentration value in 2.51 ± 0.02ug/l
Hereinafter, it is judged to slow elongated Wuding Chicken, otherwise is fast elongated Wuding Chicken；At at two monthly ages, when the concentration value of GH genes is 1.52
The concentration value of ± more than 0.07ug/l, IGF-1 gene is judged to slow elongated Wuding Chicken in 2.37 ± below 0.05ug/l, otherwise
It is fast elongated Wuding Chicken.
Further, at a monthly age, when the concentration value of GH genes is in more than 2.83ug/l, the concentration value of IGF-1 genes
In below 2.51ug/l, it is judged to slow elongated Wuding Chicken, otherwise be fast elongated Wuding Chicken；It is dense when GH genes at two monthly ages
In more than 1.52ug/l, the concentration value of IGF-1 genes is judged to slow elongated Wuding Chicken, otherwise be to angle value in below 2.37ug/l
Fast elongated Wuding Chicken.
Further, the heparin tube from the anti-coagulants containing dipotassium EDTA gathers blood, after mixing, using low temperature
3000rpm is centrifuged 10 minutes, carefully collects supernatant, obtains blood plasma.
Beneficial effects of the present invention：The present invention is long with fast in slow length by determining related gene GH and IGF-1 on growth axis
Change in concentration in type Wuding Chicken, with reference to the body weight value of the Wuding Chicken of measurement, sets up blood biochemical markers, is the early stage of Wuding Chicken
Breeding is provided fundamental basis, and is that Yunnan local chicken breeds are carried in breeding work while being also to explore Wuding County's a breed of chicken new method
For new approaches.
Specific embodiment
Embodiment 1
1. the measured body weight of slow elongated and fast elongated Wuding Chicken
A monthly age and two monthly ages slow elongated and fast elongated Wuding County are randomly selected from Yunnan Prov Agriculture University's Wuding Chicken resource population
Each 90 of chicken (each 45 an of monthly age, each 45 of two monthly ages), measures its body weight value respectively.
2. the collection of blood and the preparation of blood plasma
Heparin tube from the anti-coagulants containing dipotassium EDTA collects blood, after mixing, 10 is centrifuged using low temperature 3000rpm
Minute, carefully collect supernatant, as required blood plasma.
3. Wuding Chicken growth related gene enzyme linked immunological (ELISA) analysis
According to chicken growth factor (GH), chicken growth hormone associated proteins (GHBP) and chicken IGF (IGF-
1) ELISA (ELISA) kit carries out strictly regulating operation, using the phase of chicken in double antibody sandwich method determination sample
The level of correlation gene, then with ELIASA under 450nm wavelength mensuration absorbance (OD values), finally by standard curve calculate sample
Chicken growth factor (GH), chicken growth hormone associated proteins (GHBP) and chicken IGF (IGF-1) content in product.
4. result is counted
The monthly age of table 1 one and two monthly ages Wuding Chicken average weight (g)
Note：Colleague's data, capitalization represents difference extremely significantly (P ＜ 0.01)
The content of 2 one monthly age of table Wuding Chicken growth related gene GH, GHBP and IGF-1
The content of 3 two monthly age of table Wuding Chicken growth related gene GH, GHBP and IGF-1
The monthly age of watch 4 one slow chicken growth related gene and body weight correlation analysis
Note：* represents significantly correlated in 0.01 level (bilateral)；* represent significantly correlated in 0.05 level (bilateral).
The monthly age of watch 5 two slow chicken growth related gene and body weight correlation analysis
Note：* represents significantly correlated in 0.01 level (bilateral)；* represent significantly correlated in 0.05 level (bilateral).
The monthly age of watch 6 one fast chicken growth related gene and body weight correlation analysis
Note：* represents significantly correlated in 0.01 level (bilateral)；* represent significantly correlated in 0.05 level (bilateral).
The monthly age of watch 7 two fast chicken growth related gene and body weight correlation analysis
Note：* represents significantly correlated in 0.01 level (bilateral)；* represent significantly correlated in 0.05 level (bilateral).
8 one monthly age of table speed chicken growth related gene and body weight correlation analysis
Note：* represents significantly correlated in 0.01 level (bilateral)；* represent significantly correlated in 0.05 level (bilateral).
9 two monthly age of table speed chicken growth related gene and body weight correlation analysis
Note：* represents significantly correlated in 0.01 level (bilateral)；* represent significantly correlated in 0.05 level (bilateral).
5. interpretation of result
From table 1,2 and 3, the average weight and growth related gene GH, GHBP of a monthly age and two monthly age Wuding Chickens and
The content of IGF-1, wherein the body weight pole of a monthly age and two monthly ages fast chicken is significantly higher than slow chicken (P ＜ 0.01)；By by January
Age and two monthly age Wuding Chicken growth related genes understand that GH and IGF-1 is related in significant negatively correlated with body weight correlation analysis
Coefficient is -0.300 (table 4)；GH and IGF-1 is -0.490 in extremely significant negative correlation, coefficient correlation；GHBP is manifested with IGF-1
The positive correlation of work, coefficient correlation is 0.340 (table 5)；GH and GHBP is in extremely significant positive correlation, and coefficient correlation is 0.401 (table
6)；Except body weight is related without conspicuousness to GH, GHBP, IGF-1, other each related genes of growth are in extremely significant correlation (table
7)；GH and IGF-1 is -0.338 (table 8) in extremely significant negative correlation, coefficient correlation；Body weight is with GH and GHBP without conspicuousness phase
Close, body weight is in extremely significant positive correlation with IGF-1, GHBP and IGF-1 is in significant positive correlation, and GH and IGF-1 is in extremely significant
Negatively correlated (table 9), note：Speed chicken in watch 8 and watch 9 refers to the summation of fast chicken and slow chicken.
Show that the change in concentration of GH and IGF-1 genes determines the life of early stage Wuding Chicken by above-mentioned correlation data result
Speed long, can be seen that according to a monthly age to the change in concentration trend of two monthly age GH and IGF-1：GH mrna concentrations value is big, Wuding Chicken
It is slow-growing；IGF-1 mrna concentrations value is big, and Wuding Chicken growth is rapid.The concentration value of GH and IGF-1 genes can be selected as early stage
Educate the blood biochemical markers of slow elongated and fast elongated Wuding Chicken.
Embodiment 2
A monthly age Wuding Chicken 100 that is same in Yunnan Wuding Chicken plant lottery body heavy phase, being in a good state of health, respectively
GH, IGF-1 the mrna concentration value in its blood are detected, statistical packet is carried out (wherein according to gained concentration value within the specific limits
GH, IGF-1 the mrna concentration value for having 10 Wuding Chickens fail to meet interval differentiation simultaneously, are not counted in statistics), obtain following
Statistics (is shown in Table 10).
10 1 monthly age of table Wuding Chicken IGF-1 mrna concentration Data-Statistics
By this 5 group of Wuding Chicken at monthly age in strict accordance with immune programme for children, raised under identical rearing conditions to two monthly ages, point
Its body weight is not measured, and each group statistics is following (being shown in Table 11).
The Wuding Chicken body weight measurements at the monthly age of table 11 2
As seen from Table 11, the 3rd group of average weight significantly increases compared with the 3rd group and the 4th group, and the three, the two, one group according to
Secondary to increase, the interval range for reasoning out the 3rd group is a significant break scope, i.e. during a monthly age, GH concentration 2.80-
2.86ug/l and IGF-1 concentration 2.49-2.53ug/l is a significant break scope.
20 chickens in the range of to the break repeat above-mentioned steps, finally give GH concentration 2.83ug/l and IGF-
1 concentration 2.51ug/l is an optimal break.
GH, IGF-1 the mrna concentration value in two monthly age Wuding Chicken serum in above-mentioned each group are detected, as a result (is shown in Table as follows
12)
12 2 monthly age of table Wuding Chicken GH, IGF-1 mrna concentration Data-Statistics
By this 5 group of two Wuding Chicken at monthly age in strict accordance with immune programme for children, raised under identical rearing conditions to three monthly ages, point
Its body weight is not measured, and each group statistics is following (being shown in Table 13).
The Wuding Chicken body weight measurements at the monthly age of table 13 3
As seen from Table 13, second group of average weight increment compared with third and fourth, five groups significantly increase, reason out second group
Interval range is a significant break scope, i.e. during two monthly age, GH concentration 1.45-1.59ug/l and IGF-1 concentration 2.32-
2.42ug/l is a significant break scope.
The chicken combination of Wuding Chicken and adjacent interval overlapping ranges in the range of to the break, then repeats above-mentioned
Step, finally gives GH concentration 1.52ug/l and IGF-1 concentration 2.37ug/l is an optimal break.
In Yunnan, other Wuding Chicken plants are repeated several times experiment, conclusion and above-mentioned experiment knot by above test procedure
By identical, conclusion is：During one monthly age, GH concentration 2.80-2.86ug/l and IGF-1 concentration 2.49-2.53ug/l be one significantly
Break scope, GH concentration 2.83ug/l and IGF-1 concentration 2.51ug/l is an optimal break；During two monthly ages, GH concentration
1.45-1.59ug/l and IGF-1 concentration 2.32-2.42ug/l is a significant break scope, GH concentration 1.52ug/l and
IGF-1 concentration 2.37ug/l is an optimal break.
Embodiment 3
1. experiment material prepares
A monthly age Wuding Chicken 70 (fast elongated and slow length are randomly choosed in the Wuding Chicken sources group of Yunnan Prov Agriculture University
Each 35 of type), two monthly age Wuding Chickens 70 (fast elongated and each 35 of slow elongated) measure its body weight respectively；Selection is with containing
The heparin tube of the anti-coagulants of dipotassium EDTA collects the blood of Wuding Chicken to be measured, after mixing, 10 points is centrifuged using low temperature 3000rpm
Clock, carefully collects supernatant, as required blood plasma.
2. the concentration value of GH, IGF-1 in serum is detected
Carry out strictly regulating operation according to GH and IGF-1 ELISAs (ELISA) kit, using double-antibody sandwich
The level of the related gene of chicken in method determination sample, then with ELIASA under 450nm wavelength mensuration absorbance (OD values), finally
By GH and IGF-1 contents in standard curve calculating sample.
When the concentration value of GH genes is in more than 2.83ug/l, the concentration value of IGF-1 genes is judged in below 2.51ug/l
Slow elongated Wuding Chicken, on the contrary it is fast elongated Wuding Chicken；At at two monthly ages, when the concentration value of GH genes is in more than 1.52ug/l,
The concentration value of IGF-1 genes is judged to slow elongated Wuding Chicken, otherwise be fast elongated Wuding Chicken in below 2.37ug/l.
3. comparative analysis
In one monthly age Wuding Chicken, the concentration value for having 28 GH genes of slow elongated Wuding Chicken in 35 slow elongated Wuding Chickens reaches
To more than 2.83ug/l, IGF-1 mrna concentrations value is accounted for and always determines the 80% of slow elongated Wuding Chicken number of elements in below 2.51ug/l,
And have the body weight of 26 in this 28 slow elongated Wuding Chickens in the range of 213.97 ± 2.63g, rate of accuracy reached to 92.86%；35
Only there are 27 concentration values of the GH genes of fast elongated Wuding Chicken in below 2.83ug/l in fast elongated Wuding Chicken, IGF-1 genes are dense
In more than 2.51ug/l, account for always to determine in the 77.14% of fast elongated Wuding Chicken number of elements, and this 27 fast elongated Wuding Chickens has angle value
The body weight of 24 in the range of 268.15 ± 4.21g, rate of accuracy reached to 88.89%, Average Accuracy reaches 90.875%.
In two monthly age Wuding Chickens, the concentration value for having 29 GH genes of slow elongated Wuding Chicken in 35 slow elongated Wuding Chickens reaches
To more than 1.52ug/l, the concentration value of IGF-1 genes is accounted for and always determines slow elongated Wuding Chicken number of elements in below 2.37ug/l
There is the body weight of 26 in 82.86%, and this 29 slow elongated Wuding Chickens in the range of 615.34 ± 3.65g, rate of accuracy reached is arrived
89.66%；The concentration value for having 33 GH genes of fast elongated Wuding Chicken in 35 fast elongated Wuding Chickens reaches below 1.52ug/l,
The concentration value of IGF-1 genes is accounted for and always determines the 94.29% of fast elongated Wuding Chicken number of elements in more than 2.37ug/l, and this 33 fast
There is the body weight of 31 in elongated Wuding Chicken in the range of 953.14 ± 6.82g, rate of accuracy reached to 93.93%, Average Accuracy reaches
To 91.795%.
Embodiment 4
1. experiment material prepares
Each Wuding Chicken plant in Wuding, Yunnan county samples, and (fast elongated and slow elongated are each for a monthly age Wuding Chicken totally 102
51), two monthly age Wuding Chickens totally 102 (fast elongated and each 51 of slow elongated) measure its body weight respectively；Selection is with containing EDTA
The heparin tube of the anti-coagulants of dipotassium collects the blood of Wuding Chicken to be measured, after mixing, is centrifuged 10 minutes using low temperature 3000rpm, young
It is thin to collect supernatant, as required blood plasma.
2. the concentration value of GH, IGF-1 in serum is detected
Carry out strictly regulating operation according to GH and IGF-1 ELISAs (ELISA) kit, using double-antibody sandwich
The level of the related gene of chicken in method determination sample, then with ELIASA under 450nm wavelength mensuration absorbance (OD values), finally
By chicken growth factor (GH) and chicken IGF (IGF-1) content in standard curve calculating sample.
3. comparative analysis
In one monthly age Wuding Chicken, the concentration value for having 39 GH genes of slow elongated Wuding Chicken in 51 slow elongated Wuding Chickens reaches
To more than 2.83ug/l, IGF-1 mrna concentrations value is accounted for and always determines slow elongated Wuding Chicken number of elements in below 2.51ug/l
There is the body weight of 36 in 76.47%, and this 39 slow elongated Wuding Chickens in the range of 213.97 ± 2.63g, rate of accuracy reached is arrived
92.31%；There are 42 concentration values of the GH genes of fast elongated Wuding Chicken in 51 fast elongated Wuding Chickens in below 2.83ug/l,
IGF-1 mrna concentration values reach more than 2.51ug/l, account for and always determine the 82.35% of fast elongated Wuding Chicken number of elements, and this 42 fast
There is the body weight of 38 in elongated Wuding Chicken in the range of 268.15 ± 4.21g, rate of accuracy reached to 90.48%, Average Accuracy reaches
To 91.395%.
In two monthly age Wuding Chickens, the concentration value for having 40 GH genes of slow elongated Wuding Chicken in 51 slow elongated Wuding Chickens reaches
To more than 1.52ug/l, the concentration value of IGF-1 genes is accounted for and always determines slow elongated Wuding Chicken number of elements in below 2.37ug/l
There is the body weight of 38 in 78.43%, and this 40 slow elongated Wuding Chickens in the range of 615.34 ± 3.65g, rate of accuracy reached is arrived
95%；The concentration value for having 37 GH genes of fast elongated Wuding Chicken in 51 fast elongated Wuding Chickens reaches below 1.52ug/l,
The concentration value of IGF-1 genes is accounted for and always determines the 72.55% of fast elongated Wuding Chicken number of elements in more than 2.37ug/l, and this 37 fast
There is the body weight of 33 in elongated Wuding Chicken in the range of 953.14 ± 6.82g, rate of accuracy reached to 89.19%, Average Accuracy reaches
To 92.095%.
The present invention is by determining concentration of the related gene GH and IGF-1 in slow elongated and fast elongated Wuding Chicken on growth axis
Change, with reference to the body weight value of the Wuding Chicken of measurement, sets up blood biochemical markers, for the early stage breeding of Wuding Chicken provides theoretical base
Plinth, is that Yunnan local chicken breeds provide new approaches in breeding work while being also to explore Wuding County's a breed of chicken new method.
Finally illustrate, preferred embodiment above is merely illustrative of the technical solution of the present invention and unrestricted, although logical
Cross above preferred embodiment to be described in detail the present invention, it is to be understood by those skilled in the art that can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (1)
1. a kind of method based on GH and IGF-1 gene blood biochemical markers seed selection Wuding Chickens, it is characterised in that：Specific steps
For：
1) blood of Wuding Chicken to be measured is gathered, serum is prepared, the concentration value of GH, IGF-1 in detection serum；
2) by step 1) concentration value and the Wuding Chicken blood biochemical markers that measure are contrasted, Wuding Chicken to be measured are distinguished as soon
Elongated Wuding Chicken and slow elongated Wuding Chicken；The estblishing step of the Wuding Chicken blood biochemical markers is：
A, the slow Wuding Chicken family long of random selection and fast Wuding Chicken family long from Wuding Chicken sources group, measure body weight respectively,
Heparin tube from the anti-coagulants containing EDTA dipotassiums gathers blood, and after mixing, 3000rpm is centrifuged 10 minutes, in collection
Clearly, blood plasma is obtained；
B, from GH, GHBP and IGF-1 enzyme-linked immunoassay kit, using the phase of chicken in double antibody sandwich method determination sample
The level of correlation gene, then with ELIASA under 450nm wavelength mensuration absorbance, finally by standard curve calculate sample in
The content of GH, GHBP and IGF-1；
C, according to step b the data obtaineds, draw the concentration of slow elongated Wuding Chicken and fast each growth related gene of elongated Wuding Chicken
Value, further according to the body weight value of selected Wuding Chicken, carries out the correlation analysis of growth related gene and body weight；
D, the blood biochemical markers that Wuding Chicken is determined according to the analysis result obtained by step c；
The blood biochemical markers of described Wuding Chicken for GH, IGF-1 gene concentration value at a monthly age, when the concentration of GH genes
Value in more than 2.83 μ g/l, and IGF-1 genes concentration value in below 2.51 μ g/l, be judged to slow elongated Wuding Chicken, otherwise be
Fast elongated Wuding Chicken；At at two monthly ages, when the concentration value of GH genes is in more than 1.52 μ g/l, the concentration value of IGF-1 genes exists
Below 2.37 μ g/l, are judged to slow elongated Wuding Chicken, otherwise be fast elongated Wuding Chicken.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511017339.3A CN105424919B (en) | 2015-12-29 | 2015-12-29 | A kind of method based on GH and the gene blood biochemical markers seed selection Wuding Chickens of IGF 1 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511017339.3A CN105424919B (en) | 2015-12-29 | 2015-12-29 | A kind of method based on GH and the gene blood biochemical markers seed selection Wuding Chickens of IGF 1 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105424919A CN105424919A (en) | 2016-03-23 |
| CN105424919B true CN105424919B (en) | 2017-06-30 |
Family
ID=55503261
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201511017339.3A Active CN105424919B (en) | 2015-12-29 | 2015-12-29 | A kind of method based on GH and the gene blood biochemical markers seed selection Wuding Chickens of IGF 1 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105424919B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107711685A (en) * | 2016-08-05 | 2018-02-23 | 江苏军曼农业科技有限公司 | High-quality high anti-fast numerous new product chicken genescreen and combined method |
| EP3623810A1 (en) * | 2018-09-14 | 2020-03-18 | Institut National de la Recherche Agronomique | Serum color as biomarker of digestive efficiency of poultry |
| CN109855983A (en) * | 2018-12-21 | 2019-06-07 | 云南农业大学 | A method of based on osteoprotegerin blood biochemical markers breeding Wuding Chicken |
| CN109601477A (en) * | 2018-12-21 | 2019-04-12 | 云南农业大学 | A method of based on vitamin D receptor blood biochemical markers breeding Wuding Chicken |
| CN109540818A (en) * | 2018-12-21 | 2019-03-29 | 云南农业大学 | A method of based on calcium, phosphorus blood biochemical markers breeding Wuding Chicken |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080038742A1 (en) * | 2006-07-14 | 2008-02-14 | Porter Tom E | Genetic polymorphisms associated with body fat |
| CN103276090B (en) * | 2013-05-30 | 2013-12-11 | 扬州大学 | Jinghai yellow chicken 16-week weight molecular genetic marker and application thereof |
| CN104502615A (en) * | 2014-12-24 | 2015-04-08 | 东北农业大学 | Serum biochemical marker for auxiliary screening of low-fat broiler chicken and application of serum biochemical marker |
| CN104642264B (en) * | 2015-02-05 | 2017-02-15 | 河南农业大学 | Fast balance breeding method for native hen varieties |
| CN104894289B (en) * | 2015-07-02 | 2018-02-09 | 南阳师范学院 | For detecting the kit of chicken growth traits and the molecular breeding method of chicken |
-
2015
- 2015-12-29 CN CN201511017339.3A patent/CN105424919B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN105424919A (en) | 2016-03-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105424919B (en) | A kind of method based on GH and the gene blood biochemical markers seed selection Wuding Chickens of IGF 1 | |
| Fenaux et al. | Five tests of food‐limited growth of larvae in coastal waters by comparisons of rates of development and form of echinoplutei | |
| Starkweather | Aspects of the feeding behavior and trophic ecology of suspension-feeding rotifers | |
| Durbin et al. | Length and weight relationships of Acartia clausi from Narragansett Bay, RI 1 | |
| King | Adaptation of rotifers to seasonal variation | |
| Campbell et al. | Growth and development rates of the copepod Calanus finmarchicus reared in the laboratory | |
| Checkley Jr | The egg production of a marine planktonic copepod in relation to its food supply: Laboratory studies 1 | |
| Pawson | Using otolith weight to age fish | |
| Sokolowski | Foraging strategies of Drosophila melanogaster: a chromosomal analysis | |
| Guisande et al. | Effect of total organic content of eggs on hatching success and naupliar survival in the copepod Calanus helgolandicus | |
| Steinwascher et al. | Influence of food quality and quantity on early larval growth of two anurans | |
| Adelman et al. | Population differences in fever and sickness behaviors in a wild passerine: a role for cytokines | |
| CN106408114B (en) | Application of multiple linear regression model in prediction of abdominal fat amount of broiler chickens or breeding of low-fat-line broiler chickens | |
| Lu et al. | A comparative study on three quantitating methods of microalgal biomass | |
| CN105510573B (en) | A kind of method based on growth axis related gene blood biochemical markers seed selection Wuding Chicken | |
| Sozcu et al. | Acute and chronic eggshell temperature manipulations during hatching term influence hatchability, broiler performance, and ascites incidence | |
| Kouame et al. | Effect of guinea fowl egg storage duration on embryonic and physiological parameters, and keet juvenile growth | |
| CN114793963A (en) | Big data controlled fish rapid embrittlement environment control method | |
| Brown et al. | An improved Woodruff buffer for estimation of lime requirements | |
| CN104714038A (en) | Serum biochemical marker for auxiliary screening of low-fat meat chickens and application of serum biochemical marker | |
| CN110646614A (en) | ELISA kit and test method for IBDV antibody, and effective antibody titer determination method | |
| Bougaran et al. | Transient initial phase in continuous culture of Isochrysis galbana affinis Tahiti | |
| Dudycha et al. | Conserved ontogeny and allometric scaling of resource acquisition and allocation in the Daphniidae | |
| Ivanova et al. | Study on Egg Productivity of Guinea-Fowls (Numida meleagris) | |
| Laing et al. | The lipid content, spatfall and subsequent growth of early and late settling hatchery‐reared Pacific oyster, Crassostrea gigas Thunberg, larvae |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Jia Junjing Inventor after: Xu Zhiqiang Inventor after: Gu Dahai Inventor after: Tong Huiquan Inventor after: Cheng Zhibin Inventor after: Dou Tengfei Inventor after: Liu Lixian Inventor after: Ge Changrong Inventor after: Wang Shanrong Inventor after: Li Zhengtian Inventor after: Rong Hua Inventor after: Li Qihua Inventor after: Huang Ying Inventor before: Ge Changrong Inventor before: Liu Lixian Inventor before: Cheng Zhibin Inventor before: Zhao Xiao Inventor before: Wang Shanrong Inventor before: Zhang Yanhua Inventor before: Dou Tengfei Inventor before: Jia Junjing Inventor before: Lin Qiuye Inventor before: Rong Hua Inventor before: Li Qihua Inventor before: Li Zhengtian Inventor before: Xu Zhiqiang Inventor before: Gu Dahai Inventor before: Cao Zhenhui Inventor before: Tong Huiquan |
|
| COR | Change of bibliographic data | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |